EHT = 15.00 K V3 µm

WD = 9 mmPhoto No. = 89

Mag = 1.84 KXDetecter = SE1

0.200.18

0.150.13

50.00

75.00100.00

125.00

80

75.75

71.5

67.25

63

(EE

)

[Y1]

B: EAP(50,100 and 150 mL) C: Tween 80

(0.1, 0.15 and 0.2% v/v)

80.62

Actual factorA: EM = 20.00

Design-expert® software

Design points above predicted valueDesign points below predicted value

49.02

X1 = B: EAPX2 = C: Tween 80

EE

0.20

0.130.15

C: Tween 80(0.1, 0.15 and 0.2% v/v)

0.18

0.10

18.3

16.25

14.2

12.15

10.1

0.20

(MD

)

[Y2]

50.0075.00

100.00B: EAP(50,100 and 150 mL)

125.00

150.00

18.02

Actual factorA: EM = 20.00

Design-expert® software

Design points above predicted valueDesign points below predicted value

9.32X1 = B: EAPX2 = C: Tween 18

MD

(EE

)

[Y1]

150.00

125.00100.00

75.0050.00

10.00.

15.0020.00

30.00A: EM(10, 20 and 30 mg) B: EAP

(50,100 and 150 mL)

77

69.5

62

54.5

47

10.00

80.62

Actual factorC: Tween 80 = 0.20

Design-expert® software

49.02

X1 = A: EMX2 = B: EAP

EE

18.2

16.2

14.2

12.2

10.2

10.0015.00

20.0025.00

30.00

150.000

125.00

100.00

75.00

50.00

B: EAP(50,100 and 150 mL)

A: EM(10, 20 and 30 mg)

[Y2]

(MD

)

MD 18.02

9.32X1 = A: EMX2 = B: EAP

Actual factorC: Tween 80 = 0.20

Design-expert® software

Time (h)

120

100

80

60

40

20

0

EM1EM2EM3EM4EM5EM6EM7EM8

0 2 4 6 8 10 12 14 16 18 20

% C

umul

ativ

e re

leas

e

0 2 4 6 8 10 12 14 16 18 20

120

100

80

60

40

20

0

Time (h)

EM9EM10EM11EM12EM13

EM16

% C

umul

ativ

e re

leas

e

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