FrequentlyAskedQuestionsabout DIM-PRO PLUS - · PDF fileIndole-3-carbinol undergoes extensive and rapid self-condensation reactions ... metabolism, evidenced by an increase in the

Frequently Asked Questions about DIM-PRO PLUSWhat is Diindolylmethane?Diindolylmethane , or DIM, is a phytonutrient, found in broccoli and other cruciferousvegetables such as Brussel’s sprouts, cabbage and cauliflower.

What does DIM do in the body?DIM influences the metabolism of estrogens in the body. This is an important healthissue because estrogen has many effects on cell growth, health and function ofestrogen responsive tissues (breast, uterus, ovaries, cervix, prostate, testes, and bone).

There are three forms of naturally occurring estrogen compounds made in the body – estradiol, estrone, and estriol.There are also xenoestrogens; synthetic estrogen-like compounds that enter the body through the ingestion and/orinhalation of pesticides, drugs (hormone replacement therapy), fuels, and plastics. Combined, these estrogensrepresent the body’s systemic estrogen pool.

Estrogen, whether synthetically derived or naturally formed, is broken down through detoxification pathways in theliver and eliminated from the body. This is a crucial step in estrogen metabolism as excessive exposure to circulatingestrogens in the blood can trigger aberrant cell growth and function.

There are two different detoxification pathways in the liver by which estrogen can be metabolized. One pathwayyields estrogen derivatives known as 2-hydroxyestrone. The other pathway yields a 16-hydroxyestrone derivative.The 16-hydroxyestrones have been associated with breast and uterine cancers, cervical dysplasia, uterine fibroids,PMS, endometriosis, and obesity. These conditions are often the result of estrogen over-stimulation or dominance inthe body.

DIM works by positively influencing the production of 2-hydroxyestrone metabolites. As DIM increases these“good” estrogen metabolites, there is a simultaneous reduction in the levels of the 16-hydroxyestrone form. Thisshift in estrogen metabolism, evidenced by an increase in the 2:16 hydroxyestrone ratio, reduces the undue influenceof the 16-hydroxyestrone metabolites on cell growth and function.

What is BioResponse-DIM?BioResponse-DIM is a patented “Phytosorb” delivery system that is the only clinically proven method known toprovide predictable absorption of DIM. In a recent human study as much as five times more diindolylmethane wasabsorbed from BioResponse-DIM, than from crystalline diindolylmethane.

Could I obtain the same healthful benefits from DIM by simply eating more broccoli?Consuming a diet rich in cruciferous vegetables is beneficial form many reasons. These vegetables are rich invitamins, minerals and fiber which promotes a healthy bowel. However, active therapeutic levels of DIM found in2 capsules of BioResponse-DIM is equivalent to consuming over two pounds of raw broccoli. Consuming thisamount of broccoli a day may be problematic for some.

Why is glycine added to DIM-PRO PLUS?Glycine is an amino acid that is utilized in Phase II detoxification pathways in the liver. Through glycination,glycine forms a conjugate with a metabolic byproduct or drug rendering the newly formed compound water solubleand ready for elimination from the body through the urine and feces. Glycine is also a precursor amino acid thatcombines with glutamic acid and cysteine to form glutathione. Glutathione conjugation is a major Phase IIdetoxification pathway that aids in the removal of toxic intermediates.

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Supplement FactsServing Size: 1 Capsule

Amount Per Capsule: % Daily ValueBioResponse DIM® - 75 mg **(A patented, enhanced bioavailability complex containing min. 25%diindolylmethane) Starch, diindolylmethane, vitamin E (d-alphatocopheryl succinate, [tocophersolan]), soy phosphatidylcholine, silica

** Daily Value not established for this ingredient.

Glycine - 425 mg **

DIM-PRO PLUSDim-Pro Plus nutritionally supports the activity of enzymes that metabolize estrogen and aspirin. Current research demonstrates that BioResponse diindolylmethane increases levels of good estrogens (2-hydroxyestrogens/2-OHE1) which tend to inhibit abnormal cell replication while reducing levels of “bad” estrogens (16-a-hydroxyestrone/16-a-OHE1 and 4-OH estrone).*

What is Dim?

Dim or 3,3’-diindolylmethane is a natural product produced during the autolytic breakdown of glucobrassicin, one of over 100 sulfur containing glucose conjugates, known as glucosinolates that occur exclusively in cruciferous vegetables such as broccoli, Brussels sprouts, cabbage, kale and mustard greens.

The immediate precursor of DIM in the plant is I3C or indole-3-carbinol, which is liberated from glucobrassicin by the action of myrosinase, an enzyme found in the cell wall of cruciferous vegetables, following disruption of the sub-cellular compartmentalization of enzyme and substrate via mastication.

Indole-3-carbinol undergoes extensive and rapid self-condensation reactions under the acidic conditions of the stomach to form several derivatives with distinctive biological activities.*

DIM is a major condensation product of I3C in vitro. DIM is also produced in vivo since it has been readily detected in the livers and feces of rodents administered I3C whereas I3C was not detected.* Biological Actions of DIM on Estrogen Metabolism

1. DIM alters estrogen metabolism in humans by up-regulating a speci�c cytochrome P450 enzyme (CYP1A1), which speeds up the conversion of estrone to 2-OH estrone (a "good" estrogen) at the expense of the conversion to 4-OH estrone (a "bad" estrogen) and 16-alpha OH estrone, also a cancer causing estrogen metabolite. Breast and prostate cancer risk increases if the ratio between 2-OH and 16-alpha OH estrone is skewed in favor of 16-alpha OH estrone production by the respective cytochrome P450 enzymes.*

2. Up-regulating CYP1A1 using DIM is especially desirable if the woman has a single nucleotide polymorphism on CYP1B1, which preferentially converts estrone into 4-OH estrone, another estrogen metabolite which increases a woman's risk for breast cancer by creating DNA adducts.*

In summary, DIM enhances the production of 2-OH estrone. As DIM increases these "good" estrogen metabolites, there is a simultaneous reduction in the levels of 4-OH estrone especially if a woman has a SNP on CYP1B1 (roughly 1 in 3 women do) and 16-alpha-OH estrone. This shift in estrogen metabolism, evidenced by an increase in the 2-OH :16 alpha OH estrone ratio and reduction in 4-OH estrone levels, reduces the risk of unregulated cell proliferation and growth and DNA adducts, respectively.*

Di�erence between DIM and BioResponse-DIM (DIM-Pro Plus by Functional Integrative Therapies)

Crystalline diindolymethane is hydrophobic. It does not go into solution easily and therefore is poorly absorbed by the human body. BioResponse-DIM, on the other hand, is a patented delivery system wherein DIM is wrapped in phosphatidyl choline (lecithin) boosting absorption as much as �ve times compared to crystalline DIM. DIM-Pro Plus by Functional Integrative Therapies contains not only BioResponse-DIM (75 mg per capsule) but also the amino acid glycine. Glycine is utilized in a Phase II detoxi�cation process called glycination, where it forms a conjugate with various naturally occurring metabolic byproducts or pharmaceutical drugs to form water soluble compounds ready for elimination from the body through the urine and feces. In addition, glycine combines with glutamic acid and cysteine to form glutathione, an important tri-peptide in the removal of toxic heavy metals like mercury, lead and cadmium, pesticides, herbicides and insecticides via glutathione conjugation, another Phase II detoxi�cation pathway.*

Who can bene�t from BioResponse DIM?

Women who are taking hormone-replacement therapy.*Women who are taking bio-identical hormones.*Women diagnosed with ER+ breast cancer and worried about recurrence.*Women considering or who have had IVF therapy.*Women who are taking birth control pills.*Premenopausal women who have severe menstrual signs and symptoms.*

Case Study 1: E�ect of Dim-Pro Plus on 2-OH estrone (2-OHE1) and 16-alpha OH estrone (16-alpha OH E1) and the 2-OH:16-alpha OH E1 ratio in a premenopause woman.Age: 47History: breast cancer in maternal grandmotherGenomic Test Result: SNP on CYP1B1Present status: no breast cancer, not on HRT or bio-identical hormones and in good healthExercise Regimen: lap swimming for 1 hour, 2 to 3 times per weekBMI: 18

Dim-Pro Plus Urinary Estrogen Metabolites (pg/ml) 2-OH:16-alpha OH E1 ratioSupplementation 2-OHE1 16-alpha-OHE1None 210 435 0.48 Dim-Pro Plus (1 cap/day) 210 274 0.77Ref range 0.40 to 1.40

Case Study 2: E�ect of Dim-Pro Plus on 2-OH estrone (2-OHE1) and 16-alpha OH estrone (16-alpha OHE1) and the 2-OH:16-alpha OH E1 ratio in a peri-menopausal woman taking topical bio-identical hormones (progesterone, estradiol, estriol).Age 52History: breast cancer in father's brother, maternal grandmother and motherGenomic Test Result: SNP on CYP1B1Present status: no breast cancer, on bio-identical hormones (progesterone and estradiol)


Case Study 3: E�ect of Dim-Pro Plus on 2-OH estrone (2-OHE1), and 16-alpha OH estrone (16-alpha-OHE1) and 2-OH:16-alpha OH E1 ratio in a postmenopausal woman taking topical bio-identical hormones (progesterone and estradiol).Age 54History: no breast cancer in family treePresent status: no breast cancer, on topical bio-identicals hormones (progesterone, estradiol and testosterone)

Dim-Pro Plus Urinary Estrogen Metabolites (pg/ml) 2-OH:16-alpha OH E1 ratioSupplementation 2-OHE1 16-alpha-OHE1None 780 307 2.31Dim-Pro Plus (1 cap/day) 378 458 0.83Ref range 0.40 to 1.40

Case Study 4: E�ect of Dim-Pro Plus on 2-OH estrone (2-OHE1) and 16-alpha OH estrone (16-alpha-OHE1) and 2-OH:16-alpha OH E1 ratio in a young premenopausal woman taking birth control pills. Age 23Present status: no breast cancer, on birth control for 4 years

Supplementation Urinary Estrogen Metabolites (pg/ml) 2-OH:16-alpha OH E1 ratio 2-OHE1 16-alpha-OHE1None 92 342 0.27 I-3-Carbinol 139 525 0.26Dim-Pro Plus (1 cap per day) 258 283 0.91Ref range 0.40 to 1.40

Toll Free: 1 (800) 753-4145 Phone: (253) 875-0413 Fax: 1 (888) 878-6193 www.medifood.info

*These statements have not been evaluated by the Food and Drug Administration.This product is not intended to diagnose, treat, cure, or prevent any disease.

Dim-Pro Plus nutritionally supports the activity of enzymes that metabolize estrogen and aspirin. Current research demonstrates that BioResponse diindolylmethane increases levels of good estrogens (2-hydroxyestrogens/2-OHE1) which tend to inhibit abnormal cell replication while reducing levels of “bad” estrogens (16-a-hydroxyestrone/16-a-OHE1 and 4-OH estrone).*

What is Dim?






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DIM-PRO PLUS
















*These statements have not been evaluated by the Food and Drug Administration.This product is not intended to diagnose, treat, cure, or prevent any disease.


What is Dim?












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DIM-PRO PLUS











What is Dim?












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Documents

FrequentlyAskedQuestionsabout DIM-PRO PLUS - · PDF fileIndole-3-carbinol undergoes extensive and rapid self-condensation reactions ... metabolism, evidenced by an increase in the