ikeken@phoenix.kobe u.ac.jp

virocontrol

(Rosellina necatrix)

dsRNA

RD

RD

87

W779 vs. W370T1 (+ ZnCl2:0.75mM)

W779 (GFP ) W370T1 (GFP )

1. Ikeda K, Inoue K, Nakamura H, Hamanaka T, Ohta T, Kitazawa H, Kida C, Kanematsu S, Park P(2011) Genetic analysis of barrage line formation during mycelial incompatibility in Rosellinia necatrix.Fungal Biology 115: 80-86.

2. Inoue K, Kanematsu S, Park P, Ikeda K (2011) Cytological analysis of mycelial incompatibility inRosellinia necatrix. Fungal Biology 115: 87-95.

3. Inoue K, Kanematsu S, Park P, Ikeda K (2011) Cytological analysis of mycelial incompatibility inHelicobasidium mompa. FEMS Microbiology Letters 315: 94-100.

4. Inoue K, Ikeda K, Kanematsu S, Park P (2010) Ultrastructural analysis of mycelial incompatibility inRosellinia necatrix. Journal of Electron Microscopy Technology for Medicine and Biology 24: 83.

5. (2012)66: 55-57.

6. Ikeda K, Inoue K, Kida C, Uwamori T, Sasaki A, Kanematsu S, Park P (2013) Potentiation ofmycovirus transmission by zinc compounds via attenuation of heterogenic incompatibility inRosellinia necatrix. Applied and Environmental Microbiology 79: 3684-3691.

7. (2013) 50:120-126.

8. 2013 JATAFF1: 9-13.

9. Uwamori T, Inoue K, Kida C, Morita Y, Park P, Nakayashiki H, Kanematsu S, Ikeda K (2015) Self- andnonself recognition during hyphal interactions in Rosellinia necatrix. Journal of General PlantPathology 81: 420-428.

10. Ohkita S, Lee Y, Nguyen Q, Ikeda K, Suzuki N, Nakayashiki H (2019) Three ourmia-like viruses andtheir associated RNAs in Pyricularia oryzae. Virology 534: 25-35.

1.2010-248960, 2011-181140

2.2016-026010

ikeken@phoenix.kobe u.ac.jp

ECM

10 m

Pyricularia oryzae

ECM

10 m

ECM(ExtracellularMatrix)

( )( )

Chryseobacterium sp.Pseudomonas sp.Sphingomonas sp.Acidovorax sp.

16S rDNA

48 h

CBB

NA

4

2468

0

log 1

0cfu

/g

0.3%+Ch

0%+Ch

0.3%ChCh

Ch0.3% 0% 0.3%

+ + -

1. Shimoi, S., Inoue, K., Kitagawa, H., Yamasaki, M., Tsushima, S., Park, P., and Ikeda, K. (2010)Biological control for rice blast disease by employing detachment action with gelatinolytic bacteria.Biological Control 55: 85-91.

2.Inoue, K., Onoe, T., Park, P., and Ikeda, K. (2011) Enzymatic detachment of spore germlings inMaganporthe oryzae. FEMS Microbiology Letters 323: 13-19.

3. (2012)In p. 67-75.

4. (2012) 57: 61-66.

5. Ikeda, K., Inoue, K., Kitagawa, H., Meguro, H., Shimoi, S., and Park, P. (2012): The role of theextracellular matrix (ECM) in phytopathogenic fungi: a potential target for disease control. In PlantPathology. Ed. by Cumagun, C. J. R., INTECH Open Access Publisher, pp. 131-150.

6. Kitagawa, H., Shimoi, S., Inoue, K., Park, P., and Ikeda, K. (2014) Durable and broad-spectrumdisease protection measure against airborne phytopathogenic fungi by using the detachment actionof gelatinolytic bacteria. Biological Control 71: 1-6.

7. Inoue, K., Kitaoka, H., Park, P., Ikeda, K. (2016) Novel aspects of hydrophobins in wheat isolate ofMagnaporthe oryzae: Mpg1, but not Mhp1, is essential for adhesion and pathogenicity. Journal ofGeneral Plant Pathology 82: 18-28. JGPP

20082008 p.74

Kitagawa, H., Inoue, K., Shimoi, S., Kitaoka, H., Park, P., and Ikeda, K. (2012) Detachment control: importance of extracellular matrix (ECM) for pathogenicity and potential target for disease protection. The 2nd Korea-Japan Joint Symposium for Plant Pathology (Fukuoka International Congress Center, Fukuoka, March) Poster award

Ikeda, K., Kitagawa, H., Inoue, K., Shimoi, S., Osaki, Y., Park, P., and Nakayashiki, H. (2013) Multiple host adhesion factors in Magnaporthe oryzae –potential target for disease control-. 6th InternationalRice Blast Conference 2013 (Jeju, Korea, August) P84.

Ikeda, K., Kitagawa, H., Shimoi, S., Inoue, K., Osaki, Y., Nakayashiki, H., Park, P. (2013) Attachment of airborne pathogens to their host; a potential target for disease control. 10th International Congress of Plant Pathology (Beijing, China, August) P18.

ikeken@phoenix.kobe u.ac.jp

Quetol

Spurr ’sd

a

g

Que

tol-8

12A

rald

ite

e

b

h

f

c

i

Spu

rr’s

Wax

Pb

Sm SmPb

U UPb

US

1. Ikeda, K., Shimoi, S., Kitaoka, H., Morita, Y., Hyon, G.-S., Meguro, H., Inoue, K., and Park, P. (2010)The occurrence of splits between plant surfaces and ambient epoxy resin is prevented by theoxidation of the epidermal cuticles with sodium perioxidate. Journal of Electron MicroscopyTechnology for Medicine and Biology 24: 19-24.

2. Ikeda, K., Inoue, K., Meguro, H., Shimoi, S., Kitaoka, H., Morita, Y., Hyon, G.-S., Kanematsu, S., andPark, P. (2010) More than 0.2% water involved in unpolymerized Spurr’s resin mixture causessectioning defects in ultrathin sections of plant and fungal cells. Journal of Electron MicroscopyTechnology for Medicine and Biology 24: 35-39.

3. Ikeda, K., Inoue, K., Kanematsu, S., Horiuchi, Y., and Park, P. (2011) Enhanced Effects of nonisotopichafnium chloride in methanol as a substitute for uranyl acetate in TEM contrast of ultrastructure offungal and plant cells. Microscopy Research and Technique 74: 825-830.3.

4. Inoue, K., Morita, Y., Kanematsu, S., Park, P., and Ikeda, K. (2012) Comparative study of ultrathinsection shrinkage in various epoxy resin blocks: sectioning from Spurr’s block causes shrinkage in thelongitudinal direction. Journal of Electron Microscopy Technology for Medicine and Biology 26: 8-11.

5. Kaku, H., Inoue, K., Muranaka, Y., Park, P., Ikeda, K. (2015) Rapid contrast evaluation method basedon affinity beads and backscattered electron imaging for the screening of electron stains. Microscopy64: 361-368.

1. Onoe, T., Horiuchi, Y., Inoue, K., Ikeda, K., and Park, P. (2009) A novel staining method for thinsections and en bloc tissues of rat kidney fixed with glutaraldehyde and osmium tetroxide usingmethanolic hafnium chloride. 6th International Symposium on Electron Microscopy in Medicine andBiology 2009 (Kobe University)

2. Shimoi, S., Ikeda, K., Kitaoka, H., Inoue, K., Hyon, G.-S., Hosogi, N., Kanematsu, S., and Park, P.(2009) Treatment of periodic acid may enhance the attachment ability between specimen surfacesand the ambient resin during ultrathin sectioning. 6th International Symposium on ElectronMicroscopy in Medicine and Biology 2009 (Kobe University)

3. Horiuchi, Y., Onoe, T., Inoue, K., Ikeda, K., and Park, P. (2009) A novel staining method ofmethanolic hafnium chloride (MHC) for uranyl acetate; staining effect of MHC for thin sections ofbacteria, fungi, human biopsies, rat tissues and plants. 6th International Symposium on ElectronMicroscopy in Medicine and Biology 2009 (Kobe University)

4. (2011)27 5 p. 41.

ikeken@phoenix.kobe u.ac.jp

pH

pH

pH

pH

pHpH

pH

Mirafiori lettuce big-vein virus

Olpidium virulentus

0.5 g

pH5.0 pH6.0pH7.0 pH8.0

ISOIL for Beads Beating

100 xg 5 min

Olpidium virulentus

5 6 7 8 pH

100 xg

pH

pH

1. Iwamoto, Y., Inoue, K., Nishiguchi, S., Matsuura, K., Aino, M., Nakayashiki, H., Ikeda, K. (2017) Acidicsoil conditions suppress zoospore release from zoosporangia in Olpidium virulentus. Journal ofGeneral Plant Pathology 83: 240-243.

2. 2017 pHOlpidium virulentus

59: 51-53.

1. 2017 Olpidiumvirulentus pH 29 2017.4.26-28.

2. 2017qRT-PCR 29 2017.4.26-28.

3. 2019 Olpidium virulentus2019.3.18-19.

pH

ikeken@phoenix.kobe u.ac.jp

-factor Beef extractYeast extract

ConAWGA

weakweak

(root)hyphopodium

MAPcAMP-PKA

Hyphopodium

NADPH oxidase (Nox)

WTWT noxAnoxA noxBnoxB noxAnoxBnoxAnoxB

NoxB

1. Park, P., and Ikeda, K. (2008): Ultrastructural analysis of responses of host and fungal cells duringplant infection. Journal of General Plant Pathology 74: 2-14.

2. Hyon, G.-S., Ikeda, K., Hosogi, N., Shinogi, T., and Park, P. (2010) Inhibitory effects of antioxidantreagent in reactive oxygen species generation and penetration of appressoria of Alternaria alternataJapanese pear pathotype. Phytopathology 100: 840-847.

3. Morita, Y., Hyon, G.-S., Hosogi, N., Miyata, N., Nakayashiki, H., Muranaka, Y., Inada, N., Park, P., andIkeda, K. (2013) Appressorium-localized NADPH oxidase B is essential for aggressiveness andpathogenicity in the host-specific toxin-producing fungus Alternaria alternata Japanese pearpathotype. Molecular Plant Pathology 14: 365-378.

4. (2013): in ed. by pp.263-266.

5. Pham, K. T. M., Inoue, Y., Vu B. V., Nguyen, H. H., Nakayashiki, T., Ikeda, K., Nakayashiki, H. (2015)MoSET1 (histone H3K4 methyltransferase in Magnaporthe oryzae) regulates global gene expressionduring infection-related morphogenesis. PLoS Genetics 11: e1005385.

6. Pham Thi Minh Kieu Vu Van Ba 201550: 113-121.4.

7. Talbot JNicholas 2016

50 51: 83-94.

1. Inoue, K et al., (2013) Macroautophagy during appressorium morphogenesis in Magnaportheoryzae. 6th International Rice Blast Conference 2013 (Jeju)

2. Inoue, K et al., (2013) Autophagy-inducing condition during infection process of Magnaportheoryzae. 10th International Congress of Plant Pathology (Beijing)

3. Kieu Pham Thi Minh (2014) MoSET1, ,,

4 2018 30