GalliProBacillus based probiotic

Bacillus spore ideal as probiotic for chicken

GalliPro Comprise live Bacillus spore – natural encapsulated bacteria

Survive feed pelleting process and in feed storage

Has compatibility with all EU approved coccidiostats

Transform from spores to active cells in the intestine of chicken

Reduce FCR

Increase weight gain

Increase nutrient utilization by excretion of enzymes

Improves intestinal health and absorption capacity

Modulate intestinal microflora resulting in more Lactic Acid Bacteria

Reduce Salmonella and Campylobacter

Bacillus sp.

Definition of BacillusGram positive organismsSpore formersBacillus is facultative aerobe but at present of nitrate/nitrite it can grow anaerobic

Bacillus is found in soil and marine sediments

Bacillus as probiotic is in the form of Bacillus spore – a dormant resistant stage that can transform to a vegetative cells

Bacillus spores are pH stable at pH 2-3Trypsin stableThermo-tolerantIn-Feed stable

Bacillus spores the ideal probiotic for chicken?

Result: Viable cell count in average CFU/g feed and the recover of viable

Sample IDCalculate

dCFU/g

Meal feed

CFU/g

Pellet feed

CFU/g

Recover of cells after pelleting, %

Controltotal aerobic NA 2.6E+06 3.6E+04 1

L.farcimis NA 3.1E+04 < 10 <1

P.acidilactici 4.0E+06 3.6E+06 2.7E+04 1

E.faecium 4.0E+06 8.5E+06 1.5E+06 18

S.cereviciae 8.0E+06 8.0E+06 2.7E+04 <1

B.licheniformis 1.3E+06 1.2E+06 1.2E+06 100

GalliPro 1.3E+06 1.2E+06 1.2E+06 100

•Pelletizing at 85C 30 sec.: External facility, Bioteknologisk Institut, Denmark•Microbiological analysis: External lab, LUFA –ITL-GmbH, Germany

Pelletizing study on selected probiotic strains on the market

Bacillus spores the ideal probiotic for chicken?

Bacillus spore extremely resistant – easy to apply into feed

Bacillus spore germination occurred rapidly in the GI tracts of chicken (Cartman et al 2008)

Vegetative cells can be detected throughout the GI tract of chicks (Cartman et al 2008)

Feeding Bacillus subtilis to chicken resulted in greater growth performance and intestinal histologies such as increasing villus height. Villus tip showed no damage (Samanya & Yamauchi 2002)

Indicating improved nutrient utilization capacity

Bacillus spores the ideal probiotic for chicken?

Feeding GalliPro

More diverse and complex bacteria composition in the gut

Expectedly provides more robust microbiota less susceptible towards diseases and infections (Lan et al 2005)

High numbers of Lactic Acid BacteriaConsidered beneficial due to their putative health properties – protect against pathogens colonization and immune stimulation of the host

Lactobacillus salivarius Strong probiotic characteristics reducing Salmonella and E. coli (Pascual et al 1999, Brashears et al 2003)

Lactobacillus paracaseiStimulate the immune system of the host (Herich et al 2002, Morelli et al 2003)

GalliPro reduces the level of Campylobacter in broiler chickens

The result shows a significant reduction of Campylobacter by 52% in the GalliPro treated group.

The results on ileal samples showed a 40% reduction of Campylobacter.

Our finding of Campylobacter is accordance with literature. Corry and Atabay (2001) reported the commonly observed level in bird intestinal to be 105 to 107 CFU/g.

Quantification of campylobacter in ileum samples

gallipro group control group0,00E+00

1,00E+03

2,00E+03

3,00E+03

4,00E+03

5,00E+03

6,00E+03

7,00E+03

8,00E+03

9,00E+03

1,00E+04

CF

U/g

Quantification of Campylobacter on caecal samples

Gallipro group Control group0,00E+00

5,00E+06

1,00E+07

1,50E+07

2,00E+07

2,50E+07

3,00E+07

3,50E+07

4,00E+07

4,50E+07

cfu

/g

GalliPro significantly reduce Salmonella in chicken

Salmonella challenge pen floor study (SPR, US) with and without GalliPro showed a significantly reduction of Salmonella in the GalliPro treated group

All samples were found positive at day 42 in the control group whereas only 43% of the samples in the GalliPro group were positive with regards to Salmonella

% Salmonella positive

0

20

40

60

80

100

120

Control GalliPro

a

b

Different letters indication significant difference P< 0.05

GalliPro produce enzymesAlcalin phosphatase

Esterase

Esterase lipase

Lipase

Leucine amino-peptidase

Valine amino–peptidase

Acid phosphatase

Glucosidase

Bacillus spores the ideal probiotic for chicken?

Bacillus shows amylolitic (starch), lipolytic (fat) and proteolytic (protein) activities

Summary of 17 trialsGalliPro vs. Negative Control

Relative efficacy of GalliPro in broiler trials at independent research institutes

Avg. improvement: Final bodyweight +2,8% & FCR -3,8%

+2,8%

-3,8%

Big increases in in-vivo nutrient utilisation

ME 100% diet

Control GalliPro

Diff

Dry matter ileal digestibility (%) 74,1 78,0 3,9

Crude protein ileal digestibility (%) 82,6 85,6 3,0

Gross energy ileal digestibility (%) 77,0 81,0 4,0

N retention (%) 66,6 68,8 2,2

ME (MJ/kg DM) 13,88 14,19 0,3

ME (MJ/kg DM, N corrected) 13,46 13,76 0,3

GalliPro increased in-vivo dry matter, crude protein and gross energy digestibilities, N retention and metabolisable energy.

Study 2 at University Federal de Viçosa, Brazil

Increased in-vivo DM, CP & GE digestibilities

Increased weight gain, improved FCR and lower mortality

Canadian & EU Registration

Safe, stable and easy to use

Consistent performance

GalliPro Works!

Bacillus spores the ideal probiotic for chicken?

Bacillus subtilis (DSM17299) compatibility with Coccidiostats:

Amprolium/ethopabateDiclazurilHalofuginoneLasalocid sodiumMaduramicin ammoniumMethylbenzoquate/meticlorpindolMonensin sodiumNarasin NicarbazinNifursolRobenidineSalinomycin sodiumSemduramicin