Gel Filtration - Sorbent Technologies...filtration columns for protein purification, desalting and buffer exchange. These columns are designed for rapid and efficient removal of small

Sorbent Technologies, Inc. | www.sorbtech.com | 770-936-0323 | 5955 Peachtree Corners E, Norcross, GA 300712

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The driving force for movement through the adsorbent can be:A. Gravity – whether through classic glass columns or Polypropylene columnsB. Pressure (Flash Chromatography and FPLC) orC. Centrifugal force (Spin Columns and 96 Well Plates)

• SEC Intro • SorbaDex™ - For process purification of proteins and nucleic

acids• SorbaRose™ - An agarose-based SEC solid phase available in

4% and 6% concentrations and various surface modifications• SuperSorb™ - An agarose-dextran based SEC solid phase

available in two fractionation ranges• SorbaRes™ - A highly porous, hydrophilic polymethacrylate

matrix™ with a rigid, spherical, uniform particle size, which enables high speed, high performance production efficiency

• SorbaSep™ FPLC Columns - Desalting FPLC Columns that contain Sorbadex-25 Superfine, a beaded composite size-exclusion matrix

• SorbaRose™ FPLC Columns - Designed for desalting, removing small molecules and buffer exchange in FPLC

• Clarion™ P - Hydrated gel filtration columns for protein purification, desalting, and buffer exchange

• Clarion™ N - Hydrated gel filtration columns for nucleic acid purification and desalting

• Clarion™ Mini Spin Columns - Hydrated gel filtration columns for nucleic acid purification and desalting

• Clarion™ Gel Filtration Plates - For desalting, buffer exchange, and removal of free labels

Gel Filtration


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Size Exclusion Chromatography (SEC) is the most commonly used method to characterize the molecular weight distribution of polymer. Sorbtech’s SEC protein and nucleic acid purification products provide greater accuracy and confidence throughout development and manufacturing. This SEC media is ideally suited for numerous uses in pharmaceuticals, cosmetics, food products, and industrial products.

Choose from a range of SEC protein and nucleic acid purification products based on dextran, agarose, and agarose-extran composites, including:

SorbaDex™—(GE Healthcare Sephadex® equivalent) a cross-linked composite dextran matrix for gel filtration, buffer exchange, and desalting biological solutions. Researchers use this product to gain high performance results for proteins and nucleic acids process purification.

SorbaRose™—(Sepharose® GE Healthcare equivalent) a cross-linked beaded agarose matrix for high resolution fractionation of biomolecules, creating more versatility in separating a greater range of molecular weight materials. It’s solid bead form enables it to be poured as a slurry into columns, allowing very long columns to better separate proteins.

SuperSorb™—(Superdex® GE Healthcare equivalent) a covalently linked agarose-dextran matrix for high resolution fractionation of biomolecules. This gel filtration media is ideal for all stages of an industrial scale operation—from research and process development through scale-up and into production.

SorbaRes™—a highly porous, hydrophilic polymethacrylate matrix with a rigid, spherical, uniform particle size, which enables high speed, high performance production efficiency.

SorbaSep™—for desalting, removal of small molecules, and buffer exchange using a syringe, pump, or liquid chromatography systems, and separates larger biomolecules from unwanted smaller molecules.

Clarion™ P Columns—(gravity flow)—are hydrated gel filtration columns for protein purification, desalting and buffer exchange. These columns are designed for rapid and efficient removal of small molecules from antibodies, enzymes and other proteins. Clarion™ N Columns—(gravity flow)—are specifically designed for rapid and efficient removal of small molecules from nucleic acids.

Clarion™ MINI Spin™—the unique conical column design allows purification samples up to 100 µL—completely removing salts, dyes, haptens, and dideoxy terminators in less than five minutes. Clarion™ Gel Filtration Plates—Prehydrated plates, filled with SorbaDex S-25 or S-50, designed for desalting, buffer exchange, and removal of free labels.

Disclaimer: Sephadex®, Superdex® and Sepharose® are registered trademark products of GE Healthcare.

Conduct separation research with speed, precision, and confidence

SECGel Filtration for desalting, buffer exchange, and removal of free labels

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Separate. Purify. Discover.

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SorbaDex is a beaded composite material composed partially of cross linked dextran that exhibits high selectivity, high resolution and chemical stability. SorbaDex is a size exclusion matrix and molecules purified with SorbaDex are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal. The size exclusion cut-off for SorbaDex-25 is set at 5 kD for proteins and 10 bp for nucleic acids. For SorbaDex-50, the cut-offs are 25 kD and 20 bp. Purified biomolecules are not significantly diluted when processed using SorbaDex.

SorbaDex is autoclavable at 121°C, pH 7 for 30 minutes and is stable in all commonly used buffers, including: 0.2M NaOH; 0.2M HCl; 1M acetic acid; 8M urea; 6M guanidine HCI; 1% SOS, 24% Ethanol; 30% Propanol; and 30% Acetonitrile.

SorbaDex bulk powders are available in 100g, 500g, 1kg, 5kg, 10kg, and 100kg package sizes.

Cat No. Grade Dry Bead Size Specification801001 Superfine 20-80 µm (>80%) MWCO (size exclusion): below 5000 Da

Water regain: 2.15-2.25 mL/gSwelling: 4-6 mL/g

801002 Fine 20-80 µm (>80%)801003 Medium 50-150 µm (>80%)Inquire Coarse 100-300 µm (>80%)

Cat No. Grade Dry Bead Size Specification801004 Superfine 20-80 µm (>80%) MWCO (size exclusion): below 25000 Da

Water regain: 2.15-2.25 mL/gSwelling: 9-11 mL/g

(For the agglutination grade, the fractionation range does not apply)

801005 Fine 20-80 µm (>80%)801006 Medium 50-150 µm (>80%)Inquire Coarse 100-300 µm (>80%)Inquire Agglutination Grade 20-50 (>92.5%) (gel card)

Cat No. Description Specifications801008 S-25 Medium Hydrated in phosphate buffered saline, pH 7.4, with 0.02% NaN3Inquire S-25 Medium Hydrated in deionized water with 0.15% ProClineInquire S-50 Fine Hydrated in phosphate buffered saline, pH 7.4, with 0.02% NaN3Inquire S-50 Fine Hydrated in deionized water with 0.15% ProCline

Cat No. Description Dry Bead Size Exclusion Limit Specifications

801009 SorbaDex 20-LH 50 µm 4-5 kD Most characteristics arehighly solvent dependent801010 SorbaDex 60-LH 50 µm 20-25 kD

SorbaDex™ | SorbaDex-25 (1-5 kDa)

SorbaDex™ | SorbaDex-50 (1-30kDa)

SorbaDex™ | Pre-Hydrated Media

SorbaDex™ | Pre-Hydrated Media

SorbaDex™Gel Filtration Matrix For process purification of proteins and nucleic acids


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Grade SorbaDex-25 (Fractionation Range: 1-5kDa) SorbaDex-50 (Fractionation range: 1-30 kDa)

Super fine(Dry bread size: 20-50 µm (>80%))

Cat No. 801001 Cat No. 801004Water regain: 2.15-2.25 mL/g Swelling: 4-6 mL/g MWCO (size exclusion): below 5000 Da

Water regain: 4.80-5.20 mL/g Swelling: 9-11 mL/g MWCO (size exclusion): below 25000 Da

Fine(Dry bread size: 20-80 µm (>80%))



Medium(Dry bread size: 50-150 µm (>80%))



Coarse(Dry bread size: 100-300 µm (>80%))


Water regain: 4.80-5.20 mL/g Swelling: 9-11 mL/g MWCO (size exclusion): below 25000 D

Agglutination grade (gel card)(Dry bread size: 20-50 µm (>80%))

Cat No. Inquire

Not applicable Water regain: 4.80-5.20 mL/g Swelling: 9-11 mL/g Fractionation range does not apply

Grade SorbaDex-25 medium SorbaDex-50 fine

Pre-hydrated andready-to-use

Cat No. 801008 Cat No. InquireHydrated in phosphate buffered saline Ph 7.4, with 0.02% NaN3

Hydrated in phosphate buffered saline Ph 7.4, with 0.02% NaN3

Cat No. Inquire Cat No. Inquire

Hydrated in deionized water with 0.15% ProCline

Hydrated in deionized water with 0.15% ProCline

Grade SorbaDex 20-LH SorbaDex 60-LH

Standard(50 µm)

Cat No. 801009 Cat No. 801010Water regain: 1.9-2.1mL/g MWCO (size exclusion): 4000-5000 Da Characteristics highly solvent dependent

Water regain: MWCO (size exclusion): 20,000-25,000 Da Characteristics highly solvent dependent

For laboratory use only; not for drug, household, or other use.

SorbaDex™

High Performance Results

• Sample: Mouse IgG in PBS• Conc.: 12.5 mg/ml• Volume: 4500 ml• Total IgG: 56.25 grams• Buffer: 100 mM Glycine-HCl, pH 2.0 neutralised with 1

M Tris-HCl pH 8.0• System: ÄktaPilot (GE Healthcare)• Column: Sorbadex-25 M in BPG 300/500• Diameter: 30 cm• Gel Volume: 25 Liters• Pressure: 1.0 bar (0.1 mPa or 13 psi)• Flow rate during sample loading: 400 ml• Flow rate during equilibration and elution: 500 ml• Total run time: 63 min

Gel Filtration Matrix For process purification of proteins and nucleic acids

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Protein Desalting from 0.8M NaClIgG (280nm): dark blue lineNaCl (µS): gold line1 mg IgG anti-Rabbit in 1 mL 0.8 M sodium chlorideWater elution

Removal of FITC from IgGIgG (280nm): dark blue lineFITC (550nm): gold line1 mg IgG anti-Rabbit and 0.1 µmol FITC in 1mL DMSO/NaHCO3 PBS Elution

Oligo Desalting from 0.8M NaClOligo (260nm): dark blue lineNaCl (µS): gold line1 mg oligonucleotide (18-mer) in 1 mL 0.8 M NaCl

Removal of Rhodamine after labelingOligo (260nm): dark blue lineTAMRA (550nm): gold line1 mg oligonucleotide (18-mer) and 0.5 µmol TAMRA in 1 mL DMSO/NaHCO3

Removal of Ammonia (33%)Dextran Blue (260nm): dark blue lineNH3 (pH): gold line0.5 mg Dextran Blue (Mr 2,000,000) in 1 mL ammonia (33%). Water elution.

Removal of Ammonia (33%) from OligoOligo (260nm): dark blue lineNH3 (pH): gold line1 mg oligonucleotide (18-mer) in 1 mL ammonia (33%) after cleavage.

SorbaDex™


Gel Filtration Matrix For process purification of proteins and nucleic acids


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For more selections and sizes, visit our website or contact your personal Product Specialist.

Researchers across all industries are consistently asking for products that allows them to spend less and discover more. Sorbtech helps them achieve this with our enhanced line of Size Exclusion Chromatography products.

SorbaRose enables labs to test within a broader range of solid phases, reduce budgets, and test with confidence. Developed for high resolution fractionation of biomolecules, SorbaRose is a beaded cross-linked agarose solid phase that has varying concentrations of agarose—either 4 or 6% (w/w). Sorbtech offers unmodified and chemically modified SorbaRose solid phases suitable for all applications.

Activated solid phases use covalent bonding to selectively bind the desired complex. These phases use both the 4 and 6% concentrations of agarose and contain seven distinct modifiers to suit your needs, which bond to:

Amine • Alcohol • Carboxylic Acid • Ketones • Aldehyde • Thiol

Cat No. Description Agarose Concentration

Max Flow (mL/min) Binds to

801013 SorbaRose Aldehyde-activated FF4 4% ≤5 (-NH2)Inquire SorbaRose Aldehyde-activated FF6 6% ≤8 (-NH2)801014 SorbaRose Amine-activated FF6 6% ≤8 (-COOH, -CHO)Inquire SorbaRose Amine-activated FF4 4% ≤5 (-COOH, -CHO)801015 SorbaRose Carboxyl activated FF6 6% ≤8 (-NH2)801016 SorbaRose Carboxyl-activated FF4 4% ≤5 (-NH2)801017 SorbaRose Epoxy-activated FF4 4% ≤5 (-NH2, -SH, -OH)801018 SorbaRose Epoxy-activated FF6 6% ≤8 (-NH2, -SH, -OH)Inquire SorbaRose Hydrazide-activated FF4 4% ≤5 (-HC=O, C=O)801019 SorbaRose NHS-activated FF4 4% ≤5 (-NH2)801020 SorbaRose NHS-activated FF6 6% ≤8 (-NH2)Inquire SorbaRose Thiol-activated FF4 4% ≤5 (-SH)Inquire SorbaRose Thiol-activated FF6 6% ≤8 (-SH)

SorbaRose™

• Available for numerous applications—modified and unmodified

• Tolerant of most Clean In Place (CIP) procedures based on ligand

• Available from pilot size up to a process scale• Suitable for most solvents

• Bulk solid phases• FPLC columns• MINI Spin columns• GraviPure columns• Microplates• Column arrays• Packed SNAP columns

Advantages

Available Formats

• Antibody purification• High resolution fractionation of biomolecules• Purification of human IgG• Immobilize enzymes, antibodies, and other proteins

Recommended Applications

SorbaRose™ Activated Solid Phases

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Max Flow (mL/min) Target Ligand Density Ionic Capacity

(mmol/mL)801021 SorbaRose Heparin FF6 6% ≤8 Heparin Binding Molecules ProprietaryInquire SorbaRose Heparin HC 6% ≤4 Heparin Binding Molecules Proprietary801022 SorbaRose Protien A Endure FF4 4% ≤5 Antibody 6 mg/mL <30801023 SorbaRose Protien A FF4 4% ≤5 Antibody 6 mg/mL <30Inquire SorbaRose Protien A HC 6% ≤4 Antibody Proprietary <75801024 SorbaRose Protien G FF4 4% ≤5 Antibody Proprietary <20Inquire SorbaRose Protien L CL4 4% ≤5 Antibody 3 mg/mL <10Inquire SorbaRose Biotin CL4 4% ≤5 Avidin Streptavidin Proprietary801044 SorbaRose Co-NTA FF 6% ≤8 His-tag <15 µmol M2+ /mLInquire SorbaRose Cu-NTA FF 6% ≤8 His-tag <15 µmol M2+ /mL801045 SorbaRose Glutathione FF 4% ≤5 GST- tag <20 µmol M2+ /mLInquire SorbaRose IDA 6% ≤8 His-tag <30 µmol M2+ /mL801046 SorbaRose Ni-NTA FF 6% ≤8 His-tag <15 µmol M2+ /mL801047 SorbaRose NTA (metal-free) FF 6% ≤8 His-tag <15 µmol M2+ /mLInquire SorbaRose Streptavidin HC 6% ≤4 Biotin Proprietary801048 SorbaRose Zn-NTA FF 6% ≤8 His-tag <15 µmol M2+ /mL


Max Flow (mL/min) Functional Group Ionic Capacity

(mmol/mL)801025 SorbaRose Butyl FF4 4% ≤5 (-C4H9)801026 SorbaRose Butyl FF6 6% ≤8 (-C4H9)Inquire SorbaRose Pentyl FF4 4% ≤5 (-C5H11) <30Inquire SorbaRose Pentyl FF6 6% ≤8 (-C5H11) <30Inquire SorbaRose Hexyl FF4 4% ≤5 (-C6H13) <75Inquire SorbaRose Hexyl FF6 6% ≤8 (-C6H13) <20Inquire SorbaRose Heptyl FF4 4% ≤5 (-C7H15) <10Inquire SorbaRose Heptyl FF6 6% ≤8 (-C7H15)801027 SorbaRose Octyl FF4 4% ≤5 (-C8H17)801028 SorbaRose Octyl FF6 6% ≤8 (-C8H17)801029 SorbaRose Phenyl (High sub.) FF6 6% ≤8 (-C6H5)801030 SorbaRose Phenyl FF4 4% ≤5 (-C6H5)801031 SorbaRose Phenyl FF6 6% ≤8 (-C6H5)801032 SorbaRose Phenyl HC 6% ≤4 (-C6H5)


Max Flow (mL/min) Exchange Process Particle

Size (µm)Ionic Capacity

(mmol/mL)801033 SorbaRose CM FF6 6% ≤8 Weak Cation 100 .12801034 SorbaRose CM HC 4% ≤4 Weak Cation 35 .11801035 SorbaRose DEAE FF6 6% ≤8 Weak Anion 100 .12801036 SorbaRose DEAE HC 4% ≤4 Weak Anion 35 .11801037 SorbaRose Q FF6 6% ≤8 Strong Anion 100 .12801038 SorbaRose Q HC 4% ≤4 Strong Anion 35 .11801039 SorbaRose SP FF6 6% ≤8 Strong Cation 100 .12801040 SorbaRose SP HC 4% ≤4 Strong Cation 35 .11

Allows for rapid separation of biomolecules based on compound specific binding and interactions. Typically, these purifications result in a single pure fraction. For affinity chromatography these phases are organized by the desired target:• Antibody – For purifying antibodies and fragments• Tag – For purifying biomolecules based on linker tag interaction• General – For purifying via specific interactions not specified above

It relies on the interaction of hydrophobic surface groups of SorbaRose with the target compound. For hydrophobic interaction chromatography, the hydrophobicity typically increases based on the chain length of the modifier:

Butyl < Pentyl < Hexyl < Heptyl < Octyl < Phenyl

Similar to affinity chromatography—relies on anion and cation exchange. Sorbtech IEC solid phases are offered in 35 and 100 µm sizes with varying strengths of both anion and cation exchangers.

SorbaRose™

SorbaRose™

SorbaRose™

Affinity Chromatography

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography


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SuperSorb 753-70 kD

SuperSorb 2006-600 kD

SuperSorb 75 SuperSorb 200Exclusion Limit (Proteins, kD) 3 6Fractionation Range (Proteins, kD) 3-70 6-600Particle Size (µm) 35Flow Pressure (bar) 3-4Flow Rate (mL/min) 8-40Chemical Stability (Aqueous Buffers)

1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride,8 M urea, 30% isopropanol,

70% ethanolpH Stability 2-14 (Short term, cleaning)

3-12 (Long term, process)Autoclavable Yes

Labs are searching for cost-effective, high-performance alternatives to market-leading products to reduce budgets and improve separation resolution. Numerous researchers have turned to Sorbtech SuperSorb to enhance size exclusion and gel filtration chromatography outcomes.

SuperSorb allows labs to cut expenses without sacrificing the quality of their critical work. Developed for high resolution separation of molecules based on size, SuperSorb is a covalently-linked agarose-dextran matrix for high resolution fractionation of biomolecules and is available at a broader range than competing products.

For applications that require higher resolution, we recommened SuperSorb over SorbaDex. It delivers a much larger fractionation range designed to meet any size exclusion needs.

SuperSorb™

SuperSorb™

• Higher resolution for difficult separations• Large fractionation range to suit all applications• Unrivaled reproducibility• Perfect as a polishing step for your process• Available in Sorbtech pre-packed SNAP columns

• Bulk solid phases• FPLC columns• Packed SNAP columns

Advantages

Available Formats

• Protein desalting and buffer exchange• Antibody purification• Oligonucleotide buffer exchange• Blood fractionation• Homogeneity analysis and screening


Improve size exclusion chromatography budgets, performance, and improve results

SuperSorb 75 and SuperSorb 200

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SorbaRes Size Exclusion resins are based on a cross linked polymethacrylate resin that has been treated with a hydrophillic layer. These resins offer increased strength and flow rate. No salts are required; results are achieved with water only.

Application Data for SEC SeriesFor more selections and sizes, visit our website or contact your personal Product Specialist.

Cat No. Product Size Available Exchange Process Particle Size (µm)701001 SorbaRes SEC 120 10g, 25g, 50g Size Exclusion 10701002 SorbaRes SEC 200 10g, 25g, 50g Size Exclusion 10701003 SorbaRes SEC 600 10g, 25g, 50g Size Exclusion 10701004 SorbaRes SEC 600 Prep 100 mL Size Exclusion 30

SorbaRes resins are specially designed for the chromatographic separation of biomolecules. Based on a highly porous hydrophilic polymethacrylate matrix, the rigid, spherical, uniform particle size enables high speed chromatographic operation with high production efficiency.

SorbaRes™Gel Filtration Matrix For process purification of proteins and nucleic acids

SorbaRes SEC


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SpecificationsColumn bed volume 5ml

Size of eluted proteins >5 kD

System compatibility • Automated liquid chromatography systems (MPLC, FPLC™, AKTA™, ect.) • Peristalic Pump • Syringe

Column dimensions 1.6 cm inner diameter x 2.5 cm height

Column body material PolypropyleneColumn portsl Inlet 10-32 (1/16') Female

Outlet 10-32 (1/16') male

Support Sorbadex™-25 Superfine

Bead size 40-110 µm (hydrated)

Maximum back pressure 3 bar (0.3 MPa)

Recommended flow rate 1 to 5 ml/min

Maximum recommended flow rate 10 ml/min

Storage temperature Ambient

Storage solution 20% (v/v) ethanol/water

• Sample: 1 ml of 2 mg/ml BSA & 100 µm of in PBS pH 7.4 (0.05% NaN3)

• Flow rate: 2 ml/min.• Eluent: PBS pH 7.4 (0.05% NaN3)• Detection: Abs. at 280 nm and 490 nm


Abs

oprt

ion

Time (Minutes)

2ml/minFAM*

2ml/minBSA˚

02 48 12 1461 0

Cat No. Description Pack Size803029-5 SorbaSep FPLC Desalting 5 x 5 ml Columns

803030-100 SorbaSep FPLC Desalting 100 x 5 ml Columns

FPLC Desalting Columns | For desalting, removal of small molecules, and buffer exchange using liquid chromatography systems

The fractionation range for globular proteins is between 1 and 5 kD. The molecular weight cut-off (MWCO) is approximately 5 kD, which ensures efficient separation of proteins / peptides / biomolecules larger than 5 kD from lower molecular weight of less than 1 kD. SorbaSep Desalting FPLC Columns contain SorbaDex-25 superfine, a beaded composite size exclusion matrix. It exhibits high flow rates, excellent resolution and chemical stability. Buffer and pH effects on resolution are minimal.

SorbaRose™

• Separating larger biomolecules (i.e. proteins such as antibodies, enzymes, or larger nucleic acids) from unwanted smaller molecules

• Buffer exchange, desalting, removal of low molecular weight contaminants, and reaction terminations

• Simple, rapid and reproducible separation using a syringe pump or liquid chromatography system

Applications

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Researchers across all industries are consistently asking for products that allows them to spend less and discover more. Sorbtech helps them achieve this with our enhanced line of Size Exclusion Chromatography products.

SorbaRose enables labs to test within a broader range of solid phases, reduce budgets, and test with confidence. Developed for high resolution fractionation of biomolecules, SorbaRose is a beaded cross-linked agarose solid phase that has varying concentrations of agarose—either 4 or 6% (w/w). Sorbtech offers unmodified and chemically modified SorbaRose solid phases suitable for all applications.

SorbaRose™

• Available for numerous applications—modified and unmodified

• Tolerant of most Clean In Place (CIP) procedures based on ligand

• Available from pilot size up to a process scale• Suitable for most solvents

• Bulk solid phases• FPLC columns• MINI Spin columns• GraviPure columns• Microplates• Column arrays• Packed SNAP columns

Advantages

Available Formats

• Antibody purification• High resolution fractionation of biomolecules• Purification of human IgG• Immobilize enzymes, antibodies, and other proteins


FPLC Columns | For desalting, removal of small molecules, and buffer exchange using liquid chromatography systems

SorbaRose™ Immune-Affinity

SorbaSep FPLC Columns are designed for desalting, removing small molecules, and buffer exchange in standalone or automated HPLC systems as well as separation of larger biomolecules from unwanted smaller molecules.Cat No. Description Solid Phase Size Available Exclusion Limit803028 FPLC Desalting columns S-25SF 1mL, 5mL 5000 kD/10 BP

Cat No. Description Solid Phase Size Available Binding Capacity803029 Protein A FF SorbaRose 1mL, 5mL 25mg hlgG/mL803030 Protein A Endure SorbaRose 1mL, 5mL 25mg hlgG/mL803031 Protein A HC SorbaRose 1mL, 5mL 75mg hlgG/mL803032 Protein G FF SorbaRose 1mL, 5mL 20mg hlgG/mL803033 Protein L CL SorbaRose 1mL, 5mL 10mg hlgG/mL

SorbaRose™ FPLC Columns


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Cat No. Description Solid Phase Size Available Target803045 Q (Quaternary Ammonium) FF6 SorbaRose 1mL, 5mL Strong Anion803046 Q (Quaternary Ammonium) HC SorbaRose 1mL, 5mL Strong Anion803047 DEAE (Diethylaminoethyl) FF6 SorbaRose 1mL, 5mL Weak Anion803048 DEAE (Diethylaminoethyl) HC SorbaRose 1mL, 5mL Weak Anion803049 SP (Sulphopropyl) FF6 SorbaRose 1mL, 5mL Strong Cation803050 SP (Sulphopropyl) HC SorbaRose 1mL, 5mL Strong Cation803051 CM (Carboxymethyl) FF6 SorbaRose 1mL, 5mL Weak Cation803052 CM (Carboxymethyl) HC SorbaRose 1mL, 5mL Weak Cation

These columns are modified with different ions to have an anion or cation exchange process with the protein or compound.

These columns use covalent bonding to selectively bind the desired target to the modified SorbaRose substrate.

These columns rely on the interaction of hydrophobic surface groups of the SorbaRose substrate with the target compound.

Cat No. Description Solid Phase Size Available Target803038 IDA SorbaRose 1mL, 5mL His-Tag803039 NTA (Metal-free) FF6 SorbaRose 1mL, 5mL His-Tag803040 NTA (Nickel) FF6 SorbaRose 1mL, 5mL His-Tag803041 NTA (Cobalt) FF6 SorbaRose 1mL, 5mL His-Tag803042 NTA (Copper) FF6 SorbaRose 1mL, 5mL His-Tag803043 NTA (Zinc) FF6 SorbaRose 1mL, 5mL His-Tag803044 Glutathione SorbaRose 1mL, 5mL GST-Tag

These columns are specifically designed to target biomolecules based on linker tag interaction.

Cat No. Description Solid Phase Size Available Target803067 Adleyde-activated FF4 SorbaRose 1mL, 5mL (-NH2)803068 Adleyde-activated FF6 SorbaRose 1mL, 5mL (-NH2)803069 Amine-activated FF4 SorbaRose 1mL, 5mL (-COOH, -CHO)803070 Amine-activated FF6 SorbaRose 1mL, 5mL (-COOH, -CHO)803071 Carboxyl-actiavted FF4 SorbaRose 1mL, 5mL (-NH2)803072 Carboxyl-activated FF6 SorbaRose 1mL, 5mL (-NH2)803073 Epoxy-activated FF4 SorbaRose 1mL, 5mL (-NH2, -SH, -OH)803074 Epoxy-activated FF6 SorbaRose 1mL, 5mL (-NH2, -SH, -OH)

Cat No Description Solid Phase Size Available Functional Group803053 Phenyl (High sub.) FF6 SorbaRose 1mL, 5mL (-C6H5)803054 Phenyl FF4 SorbaRose 1mL, 5mL (-C6H5)803055 Phenyl FF6 SorbaRose 1mL, 5mL (-C6H5)803056 Phenyl HC SorbaRose 1mL, 5mL (-C6H5)803057 Butyl FF4 SorbaRose 1mL, 5mL (-C4H9)803058 Butyl FF6 SorbaRose 1mL, 5mL (-C4H9)803059 Octyl FF4 SorbaRose 1mL, 5mL (-C8H17)803060 Octyl FF6 SorbaRose 1mL, 5mL (-C8H17)803061 Pentyl FF4 SorbaRose 1mL, 5mL (-C5H11)803062 Pentyl FF6 SorbaRose 1mL, 5mL (-C5H11)803063 Hexyl FF4 SorbaRose 1mL, 5mL (-C6H13))803064 Hexyl FF6 SorbaRose 1mL, 5mL (-C6H13)

803065 Heptyl FF4 SorbaRose 1mL, 5mL (-C7H15)803066 Heptyl FF6 SorbaRose 1mL, 5mL (-C7H15)

SorbaRose™ Ion Exchange

SorbaRose™ Hydrophobic Interactions

SorbaRose™ Activated Bindings

SorbaRose™ Tagged Proteins

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Column Part Aqueous Buffer Version Solvent Resistant VersionBody Precision bore glass Precision bore glass

Pistons Acetyl PEEK

O-Rings EPDM or Viton Viton or Kalrez®

Frit Polyethylene (5 µm or 10 µm) Stainless Steel or Teflon (2 µm or 10 µm)

Temperature range 4 - 40° C 4 - 40° C

Height adjustment Short/Short, short/long, long/long pistons Short/short, short/long, long/long pistons

Connections 1/4'-28 female screw thread 1/4'-28 female screw thread

Column ID (mm)

Pressure (bars)

Pressure (PSI)

10 40 58015 35 50825 24 34835 18 26150 13 188

Maximum Pressure Rating

SNAP® | Laboratory Glass Columns FPLC Columns | For removal of small molecules, and buffer exchange using liquid chromatography systems

Liquid preparative chromatography is a widely used purification technique for a broad range of compounds, and for FPLC the common targeted molecules include proteins, peptides and nucleic acids. With the emergence of smaller particle, higher performance chromatography media, there has been an identified need for higher pressure column hardware that can handle the increased back pressure loading in a safe column configuration. Traditionally this has been addressed with stainless steel hardware, which does not allow visibility of the column contents. SNAP Laboratory Glass Columns are designed to address these evolving demands.

The column technology exceeds what is currently available. Careful choice in materials of construction, combined with customer feedback, has driven the design so that biocompatibility can be achieved in virtually any application. Simple, yet innovative design ensures frustration-free results.

"Next Generation" technology forhigh-performance preparative chromatography


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Because most columns will be wet or slurry packed, a packing adaptor should be used. SNAP column packing adaptors have a number of advantages, the first is that they are easy to install and remove. Secondly, the packing adaptors have a sufficient pressure rating, appropriate for the columns they are mounted on, allowing for adequate flow conditions. Third, they are the same diameter as the column they are mounted on avoiding issues with turbulent flow at the interface or joining point. All this adds up to ease of use, safe operations and good results!

These consist of a coupling unit and glass body of same column ID as column to be packed.Packing adaptor consists of:• SNAP coupling unit (cast resin) with seal ring insert (PTFE)• AB-Version with two Viton O-Ring• SR-Version with two Kalrez® or Viton O-Rings

Higher pressure ratingsincorporating glass construction• Pressures to 40 bars (580 psig)• Full view of bed unlike stainless steel • Rugged construction for hard lab use

Linear motion PistonDue to the true linear motion of piston there is no scraping of the bed surface or induced torsional load imposed on the packed bed assuring true linear compression.

Calibrated tubing glassSuperior glass tolerance eliminates seal adjustment. True bore tubing reduces wall effect

Robust inlet/outlet connectionsConnections are made externally and are visible if any leakage should occur.

Robust inlet/outlet connections Quick release endsConnections are made externally and are visible if any leakage should occur.

ELS (patented) column end closure design allows easy assembly and disassembly of columns even on large diameters. Inherently self-locking design ensures user safety.

Graduated glassAll columns are supplied with column graduations on the glass exterior to make bed measurements quick and simple.

ConfigurableColumns can be custom configured based on mobile phase conditions and meet the exact needs of the chromatographer.

Fine thread adjustmentColumns are provided with fine thread adjustment allowing better and more precise piston control.

True FritsColumns are supplied with true sintered material frits assuring good flow distribution and minimize dead volume.

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Advantages

SNAP® Packing Adaptor

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• Custom column lengths• Customized material configurations• Heating and cooling jackets• Custom end connections• Any unique specifications upon request

Col. ID

(mm)

Bed L.

(mm)

Press. Short/Fixed (SF) Bed

Length min-max

(mm)

Short/Fixed (SF)

Volume min-max

(mm)

Long/Fixed (LF)Bed

Length min-max

(mm)

Long/Fixed (LF)

Volume min-max

(mm)

Short/Short (SS) Bed

Length min-max

(mm)

Short/Short (SS)

Volume min-max

(mm)

Long/Short (LS)Bed

Length min-max

(mm)

Long/Short (LS)

Volume min-max

(mm)

Long/Long (LL)Bed

Length min-max

(mm)

Long/Long (LL) Volume min-max

(mm)

10

125 40 62.94-125 4.941-9.813 20.44-125 1.604-9.813 0-125 0-9.813 0-125 0-9.813 0-125 0-9.813

250 40 187.9-250 14.75-19.63 145.4-250 11.42-19.63 125.9-250 9.881-19.63 83.38-250 6.545-19.63 40.88-250 3.209-19.63

500 40 437.9-500 34.38-39.25 395.4-500 31.04-39.25 375.9-500 29.51-39.25 333.4-500 26.17-39.25 290.9-500 22.83-39.25

750 40 687.9-750 54-58.88 645.4-750 50.67-58.88 625.9-750 49.13-58.88 583.4-750 45.8-58.88 540.9-750 42.46-58.88

1000 40 937.9-1000 73.63-78.5 895.3-1000 70.28-78.5 875.9-1000 68.76-78.5 833.4-1000 65.42-78.5 790.9-1000 62.08-78.5

15

125 35 47.63-125 8.413-22.08 5.13-125 0.906-221 0-125 0-22.08 0-125 0-22.08 0-125 0-22.08

250 35 172.6-250 30.49-44.16 130.1-250 22.98-44.16 95.26-250 16.83-44.15 52.76-250 9.319-44.16 10.26-250 1.812-44.16

500 35 422.6-500 74.65-88.31 380.1-500 67.14-88.31 345.5-500 60.98-88.31 302.6-500 53.47-88.31 273-500 45.97-88.31

750 35 672.6-750 118.8-132.5 630.1-750 111.3-132.5 595.5-750 105.1-132.5 552.8-750 97.63-132.5 523-750 90.12-132.5

1000 35 922.6-1000 163-176.6 880.1-1000 155.5-176.6 845.3-1000 149.3-176.6 802.8-1000 141.8-176.6 773-1000 134.3-176.6

25

125 24 53.98-125 26.48-61.33 11.48-125 5.632-61.33 0-125 0-61.33 0-125 0-61.33 0-125 0-61.33

250 24 179-250 87.81-122.7 136.5-250 66.96-122.7 108-250 52.97-122.7 65.56-250 32.121-122.7 22.96-250 11.26-122.7

500 24 429-500 210.5-245.3 386.5-500 189.6-245.3 358-500 175.6-245.3 315.5-500 154.8-245.3 273-500 133.9-245.3

750 24 679-750 331.1-368 636.5-750 312.3-368 608-750 298.3-368 565.5-750 277.4-368 523-750 256.6-368

1000 24 929-1000 456-490.6 886.5-1000 434.9-490.6 858-1000 421-490.6 815.5-1000 400-490.6 773-1000 379-490.6

35

125 18 55.05-125 52.94-120.2 12.58-125 12.1-120.2 0-125 0-120.2 0-125 0-120.2 0-125 0-120.2

250 18 180.1-250 173.1-240.4 137.6-250 132.3-240.0 110.1-250 105.9-240.4 57.6-250 55.39-240.4 25.1-250 24.14-240.4

500 18 430.1-500 413.5-480.8 387.6-500 372.7-480.8 360.1-500 346.3-480.8 307.6-500 295.8-480.8 275.1-500 264.5-480.8

750 18 680.1-750 654-721.2 637.6-750 613.1-721.2 610.1-750 536.7-721.2 557.6-750 536.2-721.2 525.1-750 504.9-721.2

1000 18 930.1-1000 894.4-961.6 887.6-1000 853.5-961.6 860.1-1000 827.1-961.6 807.6-1000 776.6-961.6 775.1-1000 745.4-961.6

125 13 52.56-125 103.1-245.3 10.06-125 19.74-245.3 0-125 0-245.3 0-125 0-245.3 0-125 0-245.3

250 13 177.6-250 348.5-491 135.1-250 265.1-490.6 105.1-250 206.3-491 62.62-250 122.9-490.6 20.12-250 39.5-490.6

50500 13 427.6-500 839.1-981.3 385.1-500 755.5-981.3 355.1-500 696.9-981.3 312.6-500 613.7-981.3 270.1-500 530.1-981.3

750 13 605.1-750 1246-1472 562.6-750 1104-1472 520.1-750 1021-1472

1000 13 855.1-1000 1678-1963 812.6-1000 1595-1963 770.1-1000 1511-1963

Reduce overall cost by purchasing the next columns in cartridge form. Also, columns

can be stored without rotating clamps.

With the option of customized solutions

SNAP® Standard available configurations


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7.0

Con

duct

ivity

01 .0 2.04 .0 6.03.05 .0

Elution volume (ml)

Abs

oprt

ion

Elution volume (ml)

05 10 20 4015 25 30 35

Abs

oprt

ion

Removal of fluorescent dyeOval bumin (280 nm): dark blue lineFAM (490 nm): gold lineElution profile overlay of albumin (5 mg OvA) andfree dye (2.5 µmol FAM) in DMSO/NaHCO3 ,elution with water (5.0 ml sample volume).

Desalting of protein solution (1 mg anti-rabbit IgG in 1 ml 0.8 M NaCI), elution with water (dark blue line: protein-280nm; gold line: salt-µS/cm).

Clarion P gel filtration columns are specifically designed for rapid and efficient removal of small molecultes (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes, and other proteins.

Ultrapure gel and specially treated sinter frits ensure outstanding resolution and high selectivity. The gel matrix of Clarion is SorbaDex-25, a beaded composite material coprised of ultrapure cross-linked dextran. It exhibits high selectivity, high resolution and chemical stability.

Molecule puridies with SorbaDex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules.

Buffer and pH effects on resolution are minimal. The molecular weight cut-off (MWCO) for SorbaDex-25 is 5 kD for proteins. Proteins larger than 5 kD are typically purified with a 1.5-fold elution volume.

Cat. No. Description Exclusion Limit (kD) Sample Volume Bed Volume Equilibration

VolumeTypical Elution

Volume803019 Clarion™ P2 5000 50 – 300 μl 1 mL 2 mL 75 – 450 μl803020 Clarion™ P5 5000 0.5 mL 2 mL 5 mL 150 – 750 μl803021 Clarion™ P10 5000 1.0 mL 3.5 mL 10 mL 0.75-1.5 mL803022 Clarion™ P25 5000 up to 2.5 mL 8.5 mL 25 mL 2.7-3.5 mL803023 Clarion™ P50 5000 up to 5.0 mL 17.5 mL 50 mL 6-8 mL803025 Clarion™ P100 5000 up to 10.0 mL 35 mL 100 mL 12-15 mL

Hydrated gel filtration columns for protein purification, desalting, and buffer exchangeClarion™ P

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Clarion N gel filtration columns are specifically designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from nucleic acids.Ultrapure gel and specially treated sinter frits ensure outstanding resolution and high selectivity.

The gel matrix of Clarion is SorbaDex-25, a beaded composite material comprised of ultrapure cross-linked dextran. It exhibits high selectivity, resolution, and chemical stability. Molecules purified with SorbaDex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules.

Buffer and pH effects on resolution are minimal. The molecular weight cut-off (MWCO) for SorbaDex-25 is 10 bases for nucleic acids. Oligonucleotides larger than 10 bases are typically purified with 1.5-fold elution volume.

Separation of 10 M ammonia and olugonucleotide after cleavage from solid support and removal of protecting groups (18-mer, Scale: 0.2 µmol, 1 ml sample volume). Elution with water.

Elution profile overlay of 2.5 µmol 5-TAMRA and 0.25 µmol oligonucleotide (2.5 ml sample volume).

Elution volume (ml)

Abs

oprt

ion

02 61 01 41 848 12 16

Elution volume (ml)

Abs

oprt

ion

pH

00 .5 1.02 .0 3.04 .07

8

9

10

11

12

14

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1.52 .5 3.5

Cat. No. Product Exclusion Limit (kD) Sample Volume Bed Volume Equilibration

Volume Typical Elution Volume

803010 Clarion™ N2 5000 50 – 300 μl 1 mL 2 mL 75 – 450 μl803011 Clarion™ N5 5000 0.5 mL 2 mL 5 mL 150 – 750 μl803012 Clarion™ N10 5000 1.0 mL 3.5 mL 10 mL 0.75-1.5 mL803013 Clarion™ NF10 25000 1.0 mL 3.5 mL 10 mL 0.75-1.5 mL803014 Clarion™ N25 5000 up to 2.5 mL 8.5 mL 25 mL 2.7-3.5 mL803015 Clarion™ N50 5000 up to 5.0 mL 17.5 mL 50 mL 6-8 mL803017 Clarion™ N100 5000 up to 10.0 mL 35 mL 100 mL 12-15 mL803018 Clarion™ N500 5000 up to 50.0 mL 175 mL 400 mL 60-75 mL

Hydrated gel filtration columnsfor nucleic acid purification and desaltingClarion™ N


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Clarion™ MINI Spin ColumnsCentrifuge columns for desalting, buffer exchange, and removal of free labels

Cat No. Description Solvent Exclusion Limit (kD)

Exclusion Limit (BP)

Bed Volume (mL)

Optimal Sample Volume (µL)

803001 Desalt S-25 diH2O 5000 10 0.5 50803005 Desalt AZ S-25 diH2O with 0.2% sodium azide 5000 10 0.35 50803006 Desalt S-50 diH2O 25000 20 0.5 50

These columns are used for quick and efficient desalting, buffer exchange and/or removal of dyes and small molecules from proteins greater than 5 kD. Purified proteins are eluted into pure, deionized water (Caution! Some proteins may precipitate in pure water with low ionic strength!). The desalt AZ S-25 columns are stabilized with 0.02% sodium azide.

These columns are designed specifically for cleanup and sequencing reactions, with a buffer specific to your reaction need.

Cat No. Description Solvent Exclusion Limit (kD)

Exclusion Limit (BP) Bed Volume (mL) Optimal Sample

Volume (µL)803003 Tris S-25 1mM Tris buffer, pH 8 5000 10 0.5 50803004 PBS S-25 PBS buffer, pH 7 5000 10 0.5 50803007 SEQ S-50 diH2O 25000 20 0.5 50803008 Tris S-50 1mM Tris buffer, pH 8 25000 20 0.5 50803009 PBS S-50 PBS buffer, pH 7 25000 20 0.5 50Inquire BOR S-25 50mM NaB(OH)4, pH 8 5000 10 0.5 50Inquire BOR S-50 50mM NaB(OH)4, pH 8 25000 20 0.5 50Inquire TE S-50 TE buffer, pH 8 25000 20 0.5 50Inquire STE S-50 STE buffer, pH 8 25000 20 0.5 50Inquire SSC S-25 SSC Buffer, pH 7 5000 10 0.5 50Inquire SSC S-50 SSC buffer, pH 7 25000 20 0.5 50

Clarion MINI Spin columns are pre-packed with either SorbaDex-25 or SorbaDex-50 and are hydrated with various buffers for your optimal research conditions. These quick and easy to use columns are ideal for rapid processing of your PCR, DNA, and/or nucleic acid needs. All MINI Spin columns are designed for desalting compounds up to 100µL and are pre-swollen and ready to use.

Centrifuge columns for desalting,buffer exchange, and removal of free labels

Desalting Applications

Purification and Sequencing Applications

Clarion™ MINI Spin Columns

Clarion™

Clarion™

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Clarion N gel filtration plates are precision filled with SorbaDex, a beaded composite material comprised of ultrapure cross-linked dextran. They are specifically designed for desalting, buffer exchange and rapid removal of compounds, such as dye terminators, dNTPs, salts, nucleic acid fragments, biotin and other low molecular weight haptens.

SorbaDex exhibits high selectivity, high resolution and chemical stability. The molecular weight cut-off (MWCO) for SorbaDex-25 is 5 kD for proteins and 10 bases for nucleic acids. For SorbaDex-50, the cut-offs are 25kD and 20 bases respectively. Standard Clarion plates are hydrated with sterile purified water without preservatives, salts or buffers.

The filtration plate is made from sterile medical-grade polypropylene. Each gel bed is supported on an individual ultra high molecular weight PE filter membrane with pore size of 25 microns. Products may be collected into standard 96 or 384 well format collection plates (not supplied) for subsequent processing.

The purification protocol consists of a simple two to three minute spin to remove excess fluid from the wells. The samples are then loaded to the plate and spun again for two to three minutes to purify without significant dilution. Total hands on time is under seven minutes.

Cat No. Description No. of Wells Well Vol. Plate

Height

Max Sample

Vol.

Gel Bed Vol.

MatrixDrip

Director length

Proteins / NucleicAcids >

Mode of Operation

802009 Clarion 384-125LD50Gel Filtration Plate

384 140µL 15mm 10µL 100µl S-50SF Long 25 kD/20 bases Centrifuge 3 minutes

at 1000xG802002 Clarion 96-400SD50

Gel Filtration Plate96 400µL 20mm 20µL 320µl S-50SF Short 25 kD/20 bases Centrifuge 3

minutesat 1000xG

802003 Clarion 96-400LD50Gel Filtration Plate

96 400µL 20mm 20µL 320µl S-50SF Long 25 kD/20 bases Centrifuge 3 minutes

at 1000xG802004 Clarion 96-800SD50

Gel Filtration Plate96 800µL 31mm 30µL 400µl S-50SF Short 25 kD/20 bases Centrifuge 3

minutesat 1000xG

802005 Clarion 96-800SD25Gel Filtration Plate

96 800µL 31mm 30µL 400µl S-50SF Short 5 kD/10 bases Centrifuge 3 minutes

at 1000xG802007 Clarion 96

Gel Filtration Plate96 1000µL 38mm 40µL 650µl S-50SF Long 25 kD/20 bases Centrifuge 3

minutesat 1000xG

802001 Clarion 96Gel Filtration Plate

96 2000µL 44mm 80µL 1300µl S-50SF Long 25 kD/20 bases Light Vacuum

For desalting, buffer exchange,and removal of free labelsClarion™ Gel Filtration Plates

Documents

Gel Filtration - Sorbent Technologies...filtration columns for protein purification, desalting and buffer exchange. These columns are designed for rapid and efficient removal of small