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Gel pictures of cloning lab
Section1 Gel1
3 kb
5 kb
1.6 kb
M 1 2Group1 Group2 Group3
M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)
Section1 Gel2M 1 2
Group1 Group2
M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)
5 Kb
3 Kb
1.6 Kb
Section1 Gel3
3 kb
5 kb
1.6 kb
M 1 2Group1 Group2 Group3
M: DNA 1Kb ladder; 1: Purified cut vector; 2: Purified insert (CAT cassette)
Section1 “control gel”M 1 2 3 4 5 6
M: DNA 1Kb ladder; 1: Purified cut vector (it did not work. But the band should be in the position of red bar); 2:Cut vector (not purified); 3:uncut vector (labeled as PBS); 4: Purified insert (CAT cassette); 5: Cut insert plasmid (not purified); 6: uncut insert plasmid (labeled as CAT)
5 kb3 kb
1.6 kb
Section2 Gel1M 1 2 Group1 Group2 Group3
M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)
5 Kb
3 Kb
1.6 Kb
Section2 Gel2M 1 2 Group1 Group2 Group3
M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)
5 Kb
3 Kb
1.6 Kb
Section2 Gel3M 1 2 Group1
M: DNA 1Kb ladder; 1: cut vector (labeled as PBS); 2: Cut insert plasmid (labeled as CAT)
5 Kb
3 Kb
1.6 Kb