Lab 1 – Measurement and Microscopy

Welcome to Lab!

Be sure to familiarize yourself with safety procedures

○ Where does broken glass go?○ What goes in the biohazard?○ Etc.

Be sure to wear closed-toed shoes to lab everytime!

Properly reading Graduated Cylinder Always use graduated cylinder to

measure volumeDo not use a beaker!

Metric System Conversions are in units of ten Universal – used in all of the

sciences

Metric System

Used in measurements of:Length – MeterWeight – GramVolume – LiterTemperature – Celsius°C = (°F – 32°)/1.8

Important Equations to Know

Area of a block = Length x width

Volume of a block = Length x width x height○ 1cm3 (cc) = 1ml

Density = Mass/Volume

Displacement volume =Total volume (after object) – Original volume (before

object)

Temperature conversions:°C = (°F – 32°)/1.8

Practice Problems

1. 51 cm = ______mm

2. 92 cm and 1 mm = ______mm

3. 72 mm = _____cm

4. 317 m = _____cm

5. 1.35 km = _____m

6. 4156 cm = _____m

7. 8000 m = ______km

8. 83 g = _____mg

1. 976 g = _____mg

2. 4000 mg = _____g

3. 3000 g = _____kg

4. 8 L = _____ml

5. 1.75 L = _____ml

6. 985 ml = _____L

7. 7428 ml = _____L

Practice Problem Answers1. 510 mm

2. 921 mm

3. 7.2 cm

4. 31700 cm

5. 1350 m

6. 41.56 m

7. 8 km

8. 83000 mg

1. 976,000 mg

2. 4 g

3. 3 kg

4. 8000 ml

5. 1750 ml

6. 0.985 L

7. 7.428 L

Microscopy Light Microscope

Binocular dissecting microscopeCompound light microscope

Electron MicroscopeTransmission Electron Microscope

○ Thin slices of specimen

Scanning Electron Microscope○ Image of surface of specimen

The Objective Lenses Scanning Objective (4x)

Red color band, smallest one Low-power objective (10x)

Yellow color band High-power objective (40x)

Blue color band Oil Immersion objective (100x)

Gray color bandOnly use with oil! Have to carefully clean

oil off afterwards!!!!!

Magnification

Magnification = ocular x objectiveOcular lense is usually 10

Examples:○ Under 4x objective – magnification of specimen

is 40 x○ Under 10x objective – magnification of

specimen is 100x○ Under 40x objective – magnification of

specimen is 400x

Microscopy Using the Microscope

Find the microscope # assigned to you in the cabinet Carry the microscope with BOTH hands back to the lab bench Plug it in, turn on light Place slide on stage Always view specimen on lowest power first!

○ Focus and center Switch to higher power

○ Focus and center, etc. When done:

Switch lens to lowest power!! Take slide off Clean lens with only lens paper Turn light off Unplug wrap cord around base Place back in cabinet

Things to Remember

Be careful with Microscope Never turn head around! Always clean lenses with lense

paperIn your drawer

You can clean slides with Kimwipes Always put slides back in slide box

in numerical order!!!!!

Learn parts of the compound microscope that we will use

Learn how to make a wet mountRefer to Figure 2.9

Metric SystemBe comfortable with measurements and

know how to do conversions!