General Facts About New Research of Bacteria in 12

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    Smoking harm most of ourorgan

    Ex.

    -lung cancer

    -COPD(chromic obstructivepulmonary disease)

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    HARVEST

    DRYING

    HUMIDIFYING AND TEARING

    SORTING AND TRANSPORTATION

    DRYING AND HUMIDIFYING

    AGING, FERMENTING

    HUMIDIFYING.REMOVING STALKS

    MIXING.

    GRINDING AND ADDITIONS

    ROLLING

    CUTTING

    INSERT FILTER

    PACKING

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    Its well known that

    cigarette smoke harbourshundreds of toxic chemicals

    that are bad for your health.

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    But a University of Maryland environmental

    health researcher says that is not the only

    danger because:

    DNA examination of four cigarette

    brands shows, for the 1st time, that

    cigarettes are widely contaminated with

    hundreds of different types of bacteria.

    In fact, there appears to be as manybacteria in cigarettes as there are chemicals.

    (quantity of) chemical = bacteria

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    "Now that we've shown that apack of cigarettes is loaded

    with bacteria, we will conduct

    follow-up research todetermine the possible roles

    of these organisms in tobacco-related diseases." Sapkotasays.

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    The commercially available cigarettes that we tested were chock

    full of bacteria, as we had hypothesized, but we didnt think wed

    find so many that are infectious in humans, says researcher Amy

    R. Sapkota, an assistant professor in the University of Marylands

    School of Public Health.

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    PreviousPrevious researchersresearchers havehave takentaken smallsmall samplessamples

    ofof cigarettecigarette tobaccotobacco andand placedplaced themthem inin culturescultures

    toto seesee whetherwhether bacteriabacteria wouldwould growgrow..

    ButBut Sapkota'sSapkota's teamteam tooktook aa moremore holisticholistic approachapproach

    usingusing DN

    ADN

    A microarraymicroarray analysisanalysis toto estimateestimatebacterialbacterial metagenomemetagenome,, thethe totalitytotality ofof bacterialbacterial

    geneticgenetic materialmaterial presentpresent inin thethe testedtested cigarettescigarettes..

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    But,But, SapkotaSapkota andand microbialmicrobial examinedexamined thethe

    bacteriabacteria contentcontent inin 44 majormajor cigarettecigarette brandsbrands

    andand foundfound similarsimilar typestypes ofof bacteriabacteria inin eacheach

    oneone:: CamelCamel

    KoolKool Filter KingsFilter Kings

    Lucky Strike Original RedLucky Strike Original Red

    Marlboro RedMarlboro Red

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    study's findingsstudy's findings

    Commercially available cigarettes show a broad array of bacterial

    diversity, ranging from soil microorganisms to potential human

    pathogens;

    The is the first study to provide evidence that the numbers of

    microorganisms in a cigarette may be as "vast as the number of

    chemical constituents;"

    Hundreds of bacterial species were present in each cigarette, and

    additional testing is likely to increase that number significantly;

    No significant variability in bacterial diversity was observed across

    the four different cigarette brands examined.

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    NAME OF BACTERIA EFFECT OF

    Acinetobacter

    Bacillus

    Clostridium

    Klebsiella

    Pseudomonas aeruginosa

    Burkholderia

    associated with certain blood and lung infections.

    some types are associated with anthrax and food

    poisoning.

    some strains can cause respiratory infections.

    associated with many kinds of lung, blood, and

    other infections.

    linked to food poisoning-related illnesses and lung

    infections.

    a specific type of bacteria that is responsible for

    10% of hospital- acquired infections

    Bacteria of medical significance to humans were identified in all of

    the tested cigarettes and included :

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    Bacillus

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    InflammationI.Abscess on the skin, showing the redness andswellingII.Caused by endotoxinIII. structural component in the Gram negativebacteriaIV.Pseudomonas aeruginosa, Erwinia herbicolaV.Found in half of the cured tobacco leafsampleVI.Lead to atherosclerosis and rheumatoidarthritis

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    Bacteria present in tobacco leaves, but fewstudies looked into contamination

    No research has attempted to catalog the

    bacteria

    Dead bacteria produce endotoxin that canactivate cell causes inflammation

    Inflammation + chemical + bacteria = cancercells growing rapidly

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