General Microbiology Syllabus

7/28/2019 General Microbiology Syllabus

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Republic of the PhilippinesMINDANAO UNIVERSITY OF SCIENCE AND TECHNOLOGY

(Formerly Mindanao Polytechnic State College)C.M. Recto Avenue, Lapasan, 9000 Cagayan de Oro City

Tel. Nos. (088) 856-1738 / 856-1739 Telefax (088) 856-4696

SCHOOL OF ATRS AND SCIENCESDEPARTMENT OF FOOD SCIENCE AND TECHNOLOGY

General MicrobiologyCOURSE SYLLABUS

Course Code: Btech 20Course Title: General MicrobiologyCredit Units: 4 UnitsTime Allotment: 54 hrs.Course Description:

General Microbial Biology consists of both lecture and laboratory. The course will cover eukaryotic andprokaryotic microbes and viruses, but will emphasize bacteria. This course will provide a conceptual andexperimental background in microbiology sufficient to enable students to take more advanced courses in relatedfields. After taking this course, students are provided with understanding and knowledge on the value of microbes inlife rather than as disease agents. It is expected that the students will be motivated to develop their innovation onexploiting microbes for positive purposes in human living.

COURSE OBJECTIVES:

After completion of this course, students are expected to be able to understand the ubiquity ofmicroorganisms and their structure, chemistry, and metabolism. Differentiate the structural and functional

characteristics of prokaryotes and eukaryotes. Understand energy metabolism of chemotrophs and phototrophs.

Understand up-to-date molecular recombinant technology, and understand the potential of microorganisms in

industries and ecosystems.


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Course Content

TOPICS TIMEFRAM

E

SPECIFIC OBJECTIVES KEY WORDS ACTIVITYAND

LEARNINGAPPROACH

VALUES/SKILLS

INTEGRATION

RESOURCES

NEEDED

EVALUATION

MEASURES

Orientationa. VMGOb. Course

RequirementsGrading System

1 hour At the end of the class thestudents are expected to:

a. Familiarize the CollegeVMGO

b. Know the requirement ofthe course

c. Know the grading system

Appreciation andawarenessof thecollegeVMGO andgradingsystem

SyllabusCollegehandbook

Oral/written exam

Brief History of Microbiologya. What is

microbiology?b. Why studymicrobiology?

c. Careers inmicrobiology

d. Firstmicroorganisms onearth

e. Pioneers in thescience ofmicrobiology

4 hrs a. Define microbiologyb. Describe the world-

changing scientificcontributions ofLeeuwenhoek

c. Define microbes in thewords of Leeuwenhoekand as we know themtoday

d. List the four steps in thescientific method ofinvestigation

e. List the contributionsmade by Koch to the fieldof microbiology

f. List the four steps thatmust be taken to provethe cause of an infectiousdisease

g. Describe the contributionof Gram to the field ofmicrobiology

f. Identify the field ofmicrobiology that studiesthe role ofmicroorganisms in theenvironment

Microbiology

Microbes

Interactive CD

PowerpointGroupdynamics,Lecture

Toappreciate

the value ofmicroorganisms in theenvironmentAwarenesson thecontributionof thedifferentscientist inthe field ofmicrobiologyAppreciatethe value ofnaming andclassifyingorganisms

BooksLaptop

CDProjectorLCD

ReportingOral exam

WrittenexamSeatwork,Casepresentation


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Cell structure and functiona. The processes of lifeb. Eukaryotic cell VS

prokaryotic cells

8hrs a. Describe four majorprocesses of livingcells

b. Compare and contrast

prokaryotic andeukaryotic cells

c. Describe the composition,function, and relevance tohuman health ofglycocalyces

d. Distinguish betweencapsules and slime layers

e. Discuss the structure andfunction of prokaryoticflagella

f. Compare and contrast thestructures and functions

of fimbriae, pili, andflagella

g. Compare and contrast thecell walls of Gram positiveand Gram negativebacteria in terms ofstructure and Gramstaining

h. Diagram a phospholipidbilayer, and explain itssignificance in referenceto a cytoplasmicmembrane

i. Explain the fluid mosaicmodel of membranestructure

j. Compare and contrast thepassive and activeprocesses by whichmaterials cross thecytoplasmic membrane

k. Describe prokaryoticcytoplasm and its basiccontents

l. Describe the formation

CellProkaryotesEukaryotesCapsules

FlagellaPiliGrampositiveGramnegativeCytoplasmEndospore

Interactive CDPowerpoint

Groupdynamics,Lecture

To developawarenesson thenature and

function ofthe cell anditscomponent.

BooksLaptopCDProjector

LCDMicroscopePreparedslides

ReportingOral examWrittenexam

Seatwork,CasepresentationLaboratoryactivities


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and function of endosporem. Compare and contrast

prokaryotic andeukaryotic cell walls andcytoplasmic membranes

Microscopy, Staining, andClassification

a. Microscopyb. Stainingc. Classification

4hrs a. Define microscopyb. Contrast simple and

compound microscopec. Discuss the purposes of

classification andidentification oforganisms

d. Define binomialnomenclature

e. Describe a fewmodification of theLinnean system oftaxonomy

MicroscopeLinneansystem

Interactive CDPowerpointGroupdynamics,Lecture

Provideawarenessandappreciationof the use ofmicroscopeinidentifyingmicroorganisms

BooksLaptopCDProjectorLCDMicroscopePreparedslides

ReportingOral examWrittenexamSeatwork,CasepresentationLaboratoryactivities

Microbial Metabolisma. Basic chemical

reactions underlyingmetabolism

b. Carbohydratecatabolism

c. Photosynthesis

7hrs a. Distinguish amongmetabolism, anabolism,and catabolism

b. Contrast reduction andoxidation reaction

c. Draw a table listing thesix basic types ofenzymes, their activities,and an example of each

d. Describe howtemperature, pH,substrate concentration,and competitive and non-

competitive inhibitionaffect enzyme activitye. In general terms, describe

the three stages ofaerobic glucosecatabolism (glycolysis,the kryb cycle, and theelectron transport chain),including their substrates,products, and net energyproduction

f. Discuss the roles of acetyl

MetabolismAnabolismCatabolismOxidationReductionEnzymesGlycolysisKrebs cycleElectrontransportchainPhotosynthesis

DarkreactionLightreaction


Appreciation on thebasicmechanisms ofbacterialmetabolism

BooksLaptopCDProjectorLCD



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CoA, the krebs cycle, andelectron transport incarbohydrate catabolism

g. Contrast electrontransport in aerobic andanaerobic respiration

h. Describe the role ofchemiosmosis in oxidativephosphorelation of ATP

i. Define photosynthesisj. Compare and contrast the

basic chemicals andstructures involved inphotosynthesis inprokaryotes andeukaryotes

Microbial Nutrition andGrowth

a. Growthrequirements

b. CulturingMicroorganisms

c. Growth andMicrobial Population

6hrs a. Describe the roles of carbon, hydrogen,

oxygen, trace elements,and vitamins in microbialgrowth and reproduction

b. Distinguish amonganaerobes, aerobes,earotolerant anaerobes,facultative anaerobes,and microearophiles

c. Explain how extremes oftemperature, pH, andosmotic and hydrostaticpressure limit microbialgrowth

d. Describe the two mostcommon methods bywhich microorganismscan be isolated for culture

e. Describe six types ofgeneral culture mediaavailable for bacterialculture

f. Describe logarithmicgrowth

g. Explain what is meant bythe generation time of

AerobicAnaerobic

FacultativePure cultureCulturemediaLag phaseLog phasegenerationtime

Interactive CD


Developpatience in

culturingbacteriaAwarenesson theprocess ofthe growthof thebacteria

BooksLaptop

CDProjectorLCD

ReportingOral exam

WrittenexamSeatwork,CasepresentationLaboratoryactivities


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bacteriah. Draw and label a bacterial

growth curvei. Describe what occurs at

each phase ofpopulations growth

j. Contrast direct andindirect methods ofmeasuring bacterialgrowth

Controlling Microbial Growtha. Basic Principles of

Microbial Controlb. The Selection of

Microbial ControlMethods

c. Physical Methods ofMicrobial control

d. Chemical Methodsof Microbial Control

6hrs a. Contrast sterilization,disinfection,, andantisepsis, and describetheir practical uses

b. Contrast the termsdegerming, sanitization,and pasteurization

c. Compare the effects of -

static versus-cidalcontrol agents onmicrobial growth

d. Define microbial deathrate, and describe itssignificance in microbialcontrol

e. Describe howantimicrobial agents actagainst cell walls,cytoplasmic membranes,proteins, and nucleicacids

f. Identify the three most-resistant groups ofmicrobes, and explainwhy they are resistant tomany antimicrobialagents

g. Describe five types ofphysical methods ofmicrobial growth

DisinfectionSterilizationAntisepsisPasteurizationSanitizationantimicrobial


Developskills incontrollingmicrobialgrowthAppreciatethe differentmethods of

controllingmicrobialgrowth


ReportingOral examWrittenexamSeatwork,Casepresentation

Characterizing andClassifying Prokaryotes

2hrs a. Identify six basic shapesof prokaryotic cells

Interactive CD

Appreciation on the

BooksLaptop

ReportingOral exam


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a. Generalcharacteristics ofProkaryoticOrganisms

b. Modern ProkaryoticClassification

b. List three common typesof reproduction inprokaryotes

c. Describe the formationand function of bacterialendospores


value ofnaming andclassifyingmicroorganisms

CDProjectorLCD

WrittenexamSeatwork,Casepresentation

Characterizing andClassifying Eukaryotes

a. GeneralCharacteristics ofEukaryoticOrganisms

b. Fungi

3hrs a. State four reasons whyeukaryotic reproduction ismore complex thanprokaryotic reproduction

b. Cite at least threecharacteristics thatdistinguish fungi fromother groups ofeukaryotes

c. List five ways in whichfungi are beneficial

d. Distinguish among

septate hyphae, aseptatehyphae, and mycelia

e. Compare and contrast thethree divisions of fungiwith respect to theformation of sexualspores

f. List several beneficialroles of lichens

FungiHyphaeAseptateseptateMycelialichens


Value theimportanceof fungi inindustrialmicrobiology


Food Microbiologya. The Roles of

Microorganisms inFood Production

b. The Causes andPrevention of FoodSpoilage

c. Food borne Illnesses

5hrs a. Describe how microbialmetabolism can bemanipulated for foodproduction

b. Describe how foodcharacteristics and thepresence ofmicroorganisms in foodcan lead to food spoilage

c. List several methods forpreventing food spoilage

d. Discuss the basic types ofillnesses caused by foodspoilage or foodcontamination, anddescribe how they can be

SpoilageIllnesscontamination

Interactive CDPowerpoint

Groupdynamics,Lecture

Developskills inpreservingfood

BooksLaptopCDProjector

LCD

ReportingOral examWrittenexam

Seatwork,CasepresentationLaboratoryactivities


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avoided

Industrial Microbiologya. The Roles of

Microbes inIndustrialFermentatations

b. Industrial Productsof Microorganisms

c. Water andWastewater

Treatmentd. Bioremediation

7hrs a. Describe the role of genetically manipulatedmicroorganisms inindustrial and agriculturalprocesses and the basicsof industrial-scalefermentation

b. List some of the variouscommercial productsproduced bymicroorganisms

c. Explain how water andwaste water are treatedto make them safe andusable

d. Describe the process ofbioremediation

FermentationWastewaterBioremediation


Appreciation andawarenesson the roleplayed bymicrobes intheproductionofantibiotics,beer,wine,cheese,yogurt,etc.Developskills onwatertreatment



Course Requirements: Projects

Assignments

Attendance

Oral and Written Exams

Research Work

Reporting Laboratory Activities

Text Books:

Michael J. Waites et al.,2001. Introduction to Industrial Microbiology, Blackwell Science Ltd.

Nester, Anderson, Roberts, Pearsall, Nester. 2004. Microbiology: A Human Perspective, McGraw-Hill, 4 th

Edition

Burton, Engelkirk. 2004. Microbiology for the Health Sciences, Lippincott and Wilkins, 7 th Edition

Tortora, Funke, Case. 2002. Microbiology: an Introduction, Benjamin Cummings, 7th Edition


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Grading System:

Quizzes ----------------30% Final Grade:

Attendance ---------- 10% Midterm Grade ------ 30%

Participation ---------15% Final Term Grade ----70%

Assignment /Proj ---15% 100%

Term Exam -----------30%

100%

Laboratory Lecture ------------70%

Practical Exam ----20% Laboratory --------30%

Experiment --------30% 100%

Attendance --------10%

Term Exam --------40%

100%

Prepared by: Checked by: Approved by:

_______________________ _________________________

_______________________


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CORDULO P. ASCAO II, M.S. PHOEBE L. GALLONVIMA SOCORRO J. TANDOG

Course Lecturer Chairman, Food Science and Technology Dean,

College of Arts and Sciences

Documents

General Microbiology Syllabus