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Generating Extracellular Matrix (ECM)for Tissue Culture Applications
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ADIPOSE-DERIVED STEM CELLS AS A SOURCE FOR TISSUE ENGINEERING
Possible Sources:ASCs or ADSCsNomenclature: International Fat Applied Technology Society advocates use of the term, “ASCs” to refer to either “adipose-derived stem cells” or “adipose-derived stromal cells”
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ADIPOSE TISSUE STEM CELL RECOVERY
Lipoaspirate Liposuction surgeries can yield anywhere from
100 mL to >3 L of lipoaspirate tissue Independent studies have determined that
liposuction aspiration alone does not significantly alter the viability of SVF cells
When ultrasound-assisted liposuction is performed, a reduction is seen in the number of cells recovered as well as their proliferative capacity
Lipoaspirate may be a superior source of ADSCs, since it already contains finely minced fragments (generated by cannulation)
Alternatively, cadaveric adipose tissue can also be considered as a possible source.
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DECELLULARIZATION
ECMStem cells, when cultured, will secrete extracellular matrix (ECM) which, once decellularized, can be used for tissue engineering purposes and other applications."
Reference: Sun Y, et al. (2011) “Rescuing replication and osteogenesis of aged mesenchymal stem cells by exposure to a young extracellular matrix.” FASEB J 25: 1474-85.