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Splice into Cloning Vehicle
• Plasmids• Viruses• Use Restriction
Endonuclease to create –
• Sticking Ends• Rejoin Molecule with• Ligase
Cloning Vector
• Plasmid Cloning Vectors
• Single Cleavage Site
• High copy Numbers• Insertion Inactivation
Other Vectors
• Expression Vectors• Secretion Vectors• Shuttle Vectors• Bacteriophage M13• DNA Sequencing• Yeast Artificial
Chromosome• Human Genome Project
Production of Recombinant Protein
• Transcription of Cloned Gene
• Promoter
• Translation of Cloned Gene
• Ribosome Binding Site
Vaccines
• Subunit Vaccines• Hepatitis Vaccine• Recombinant Vaccines• Polyvalent Vaccines• DNA Vaccines• Edible Vaccines
Other Products
• Tissue Plasminogen Activator
• Rennin• Ti Plasmid• Bacillus
thuringiensis – BT• Roundup Resistant• Favosavr Tomato
More Possibilities
• Ice Minus Bacteria• PCB Degrading
Bacteria• Luminescent Reporter
Gene• Transgenic Animal• Human Gene Therapy
Genetic Eng. Word List
Biotechnology
Molecular Cloning
Recombinant DNA
Isolate Gene
mRNA,
Reverse Transcriptase
Splice into Cloning Vehicle -
Plasmids, Viruses
Restriction Endonucleases
Sticky Ends
Ligase
Insert into Host
Detect Successful Clone
DNA Library
Shotgun Cloning
Plasmid Cloning Vectors
Single Cleavage Sites
Genetic Eng. Word List 2
High Copy numbers
Insertion Inactivation
Lambda Phage Vector
Stable Packaging
Cosmids
Other Vectors
Expression Vectors
Secretion Vectors Shuttle Vectors
Bacteriophage M 13
DNA Sequencing
Yeast Artificial Chromosomes
Human Genome Project
Cloning Vector Hosts
E. coli
Yeast
Detection of Clones
Antibody
Genetic Eng. Word List 3Nucleic Acid Probes
Transcription of Cloned Gene
Promoter
Translation of Cloned Gene
Ribosome Binding Site
Synthetic DNA
Probes
Polymerase chain Reaction –
PCR
DNA Amplification
TAQ Enzyme
Primers
Site Directed Mutagenesis
Knockout Mutations
Applications of Genetic
Engineering
Human Insulin
Interferon
Hepatitis Vaccine
Recombinant Vaccine
Subunit Vaccines
Genetic Eng. Word List 4
Polyvalent Vaccines
DNA Vaccines
Tissue Plasminogen Activator
Rennin
Ti Plasmid
Bacillus thuringiensis
Roundup Resistance
Favosavr Tomato
Ice Minus Bacteria
PCB Degrading Bacteria
Luminescent Reporter Gene
Transgenic Animals
Edible Vaccines
Human Gene Therapy
DNA Chips – Microarrays