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Restriction Enzymes Cut DNA
sequences at specific repeating patterns.
EcoRI cuts at GAATTC which will appear at different points for different people.
Gene Therapy Desired genes are
inserted into viruses Organisms are then
“infected” by the viruses that pass the “cure” for the genetic problem onto the host cells
Transgenic Organisms with foreign DNA inserted into
their own genomes. DNA is cut with restriction enzymes, and
fragments containing desired genes are spliced into the original DNA
Gel Electrophoresis After DNA is cut with
restriction enzymes it will be run on a “gel” to separate the fragments by length.
Longer fragments move more slowly through the gel than short ones
DNA Fingerprinting
Technique of DNA comparison developed by Alec Jeffreys in Leicester England 20 years ago.
% chance of two people having identical DNA Fingerprints is 1 in 9,390,000,000, which is twice the world population
Paternity Testing The child’s DNA
fragments must be a combination of both parents.
Fragments belonging to the child will be either from mom, dad, or both.
No fragments should simply “appear” in the child
Disease Testing—Sickle Cell Anemia Hb A is an allele
that contributes “normal” red blood cells
Hb S is an allele for sickle-shaped red blood cells.
Crime Scene Investigation A woman and her fiancé were sleeping in
their car Woman was raped, both murdered and the
car stolen. The man caught with the stolen car said a
friend had given him the vehicle. Both Chris Reesh and Randall Jones
lacked alibis for the night of the murders.
Crime Scene Investigation The first case in history decided by DNA
fingerprinting. Semen from the victim
Was cut with a restriction
Enzyme and compared
to blood samples from
both suspects