Grant Application BAN04 · 2019-09-04 · Deadline$2017$–$see$isp.uu.se/documents$ $ 5$(31)$ $ credits.$Ph.D$and$M.Phil.$studentsgive$oral$presentation$of$their$research$workin$front$of$faculty

ISP Uppsala Universitet Box 549 SE-‐751 21 Uppsala, Sweden Fax +46 18 471 3495 [email protected]

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Deadline 2017 – see isp.uu.se/documents

Grant application for 2018-‐2020 Research Groups and Scientific Networks

The application should be submitted as email attachment with a scanned/photographed copy of the first signed page. Enclosures have to be attached as well. Read the separate document Guidelines for Enclosures and Budget for more information. The blue underlined words in this document will provide you with specific instructions when you hover your mouse over them. 1. Organization Program Chemistry Mathematics Physics

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Other

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Activity code Research Group code/ Network acronym BAN:04

Applicant (Research group leader/Network coordinator: title, given name, family name) PROFESSOR MOHAMMAD SHOEB Address Department/unit: Department of Chemistry University/institute: University of Dhaka Street (visiting address): Curzon Hall P.O Box number: City: Dhaka

Post/zip code: 1000 Country: Bangladesh

E-‐mail address(es): [email protected]; [email protected] Website: www.du.ac.bd Telephone and telefax Office +88029661920-‐82

Home +8802-‐9124617

Mobile +88801715191988

Fax +88029667222

Name of Research Group/Network: Studies of Organic Pollutants in Food and Environment City: Dhaka Approved by the Department: ………………………………………………………/ Date: 28 August 2017 Signature by Head of Department/Name in printing: Prof Dr. Md. Azizur Rahman

Summary of budget request (SEK) 2018 2019 2020 Total Equipment/spare parts/service 440,000 320,000 300,000 1,060,000 Consumables/literature/field work 135,000 100,000 135,000 370,000 Conferences/workshops 70,000 60,000 70,000 200,000 Exchange visits by cooperating scientists 50,000 50,000 50,000 150,000 Fellowships for training: 10,000 80,000 80,000 170,000 Support to students 80,000 80,000 80,000 240,000 Costs for audit and RG meeting 5,000 5,000 5,000 15,000 Reference group meeting participation costs 15,000 15,000 Network (only) administration costs Total 790,000 695,000 735,000 2,220,000 The following enclosures are submitted

1) Research plan/network program 2) Logical Framework Matrix 3) Application for improving gender balance 4) Fellowship application(s) 5) Applicant’s CV 6) Publications/Theses/Abstracts 7) Other (Specify:…………………………………………..........)

Yes / No Yes Yes Yes No Yes Yes Yes

City: Dhaka Date: 28 August 2017 Applicant’s signature


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2. Summary of proposed research/network activity (2018-‐2020) (Full details should be given in Enclosures 1 and 2. Please, carefully read and follow guidelines)

a) Give an overview of objectives, planned outputs and expected outcomes Provide a summary of objectives, planned outputs and expected outcomes based on what is given in Enclosure 1 (Sections a -‐ c), and formalized in the Logical Framework Matrix in Enclosure 2. Provide your answers under the below headings.

Overall objective (impact) and relevance of the activity:

Fertilizer and pesticides are being frequently used in agricultural fields in Bangladesh in order to increase the bumper production of agricultural crops. A large number of chemicals including antibiotics are also used in fish culture, chicken and beef farming. Thus, food contamination and adulteration is intentionally or unintentionaly increasing in the country. Food safety is a burning issue in Bangladesh now as toxicity of food stuff is increasing in alarming scale due to the use of toxic chemicals during food production, preservation, early fruit ripening and food storage. The situation seems to be more severe as almost every day the leading newspapers are covering a number of news about the adulteration of food, destroying huge amounts of fruits and food stuff from the market by the government officials due to the presence of unauthorized chemicals that have been used to make the food more attractive and colorful. People are very concered to consume food in Bangladesh. So, the overall objective is to improve the status of food safety and quality, and reduce environmental contamination in Bangladesh.

Recently, the Government formed the Bangladesh Food Safety Authority (BFSA) to ensure the people’s right toward access to safe food through appropriate application of scientific processes and state of the art technology. BFSA indentifed several laboratories to form Food Safety Laboratory Network and BAN:04 is one of them. Getting support from ISP and other sources, BAN:04 group members have been trained up to analyze different kinds of chemical contaminants in food and environment and laboratories facilities have been established. Postgradute students have been/will be trained up. They will develop new methods or modify exsiting methods and will analyse samples for screening of any chemical contaminants present in food and environment. Our research activity will help to increase food safety and reduce enviromental pollution by producing relevant scientific data and providing results to Food Safety Authority and other concerned authority. Hence, our objective is very much releavnt to the need of the country.

Specific objectives for 2018-‐2020:

i) to develop/establish analytical methods for identification and quantification of different toxic contaminants like pesticides, organohalogen compounds, antibiotics, aflatoxins, polycyclic aromatic hydrocarbons, microplastic, phthalates etc. in food and environmental samples.

ii) to foster post graduate education plan by giving training to Ph.D. M. Phil. and MS students and also undergraduate students, and produce skilled manpower iii) to create awareness among the farmers and food producers about the short and long term harmful effects of chemical contaminants on human health and environment.


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iv) to actively participate in the ISP supported network Asian Network of Research on Food and Environmental Contaminants (ANFEC) in order to increase the research capacity and staff training. v) to maintain and increase South-‐South and North-‐South collaborations by staff and student exchange programme. vi) to disseminate the research finding by publishing scientific papers in peer reviewed journals and organizing/participating seminar/conferences. vii) to contribute to the Government/society during policy making by giving scientific data and suggestion to improve food safety and reduce environmental pollution.

Expected outcomes of the research activity:

-‐ Scientific Results: Existing methods will be modified and new methods will be devoloped and validated. Samples (fish, soil, water, vegetables, chicken, beef, and processed food etc.) will be analyzed for the presence of residual contaminants and results will be included in the students’ theses.

-‐ Graduations: During last granting period (2015-‐2017), a total of 38 students including Ph.D. (6), M.Phil. (1), MS (15) and BS (4th Year project; 16) completed research work, submitted theses and finally graduated. It is expected that similiar number of students (n=38) will carry out research and produce theses, and will be graduated after their degree programme.

-‐ Dissemination (publications/conference contributions, etc.): Research findings will be published in peer reviewed journals and presented in local, regional and international seminar, symposium or workshops.

-‐ -‐ Scientific exchange: South-‐South and North-‐South exchange visit of staff members

and post-‐graduate students will be arranged. ANFEC Fellow from Laos and Cambodia and Minor Field Study (MFS) students from Sweden or other country will be welcome. Scientists from the North and/or from South will be invited to deliver lectures on current issues of food contaminants and environmental pollutions.

-‐ Interactions with government and society: Scientific results will be shared with

BFSA/governmental officials/policy makers/media by means of meeting/seminar/conference. Meeting and workshop with farmers will be arranged in rural level to create awareness about safe use of chemicals and their toxic effects on human health and environment.

-‐ Other: Female students will be encouraged to participate in training and seminar to

improve gender balance.


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b) Give a summary of the research plan/network program Give a maximum half a page summary of the proposed activity with emphasis on the research plan, sufficiently concrete and informative to give the reader a fair understanding of which research questions are addressed, including the methodology, and the hypotheses to be tested. Please note that this is a summary of Enclosure 1, Sections a -‐ e.

Summary:

Bangladesh is an agricultural country with a population of 160 million in 56,000 square miles. In order to meet the demand of food supply for a huge of number population, a large number of fertilizer and pesticides are being used in agricultural crops. Antibiotics are also being frequently used in fish culture, chicken and beef farming. Thus, food safety is a burning issue in Bangladesh now as toxicity of food stuff is increasing in alarming scale in Bangladesh due to the use of toxic chemicals during food production, preservation, early fruit ripening and food storage. Environmental pollution is another major problem in the country. Pesticides and fertilize usually discharge in the water body from the agricultural fields. So, the research questions may arise: i) Are food safe to consume; ii) What are the level of contamination if any; iii) How to improve the food safety; iv) Do farmers use proper dose of pesticide and maintain pre-‐harvest interval; v) What is the situation of environmental pollution; vi) How to reduce the pollution; vii) How to improve laboratory facility and produce skill manpower to face the problem? Recently, the Government formed the Bangladesh Food Safety Authority (BFSA) to ensure safe food for all and our group wishes to actively help them by scientifc results. So the overall objective is to improve the status of food safety & quality, and reduce environmental pollution in Bangladesh. To achieve this, the specific objectives are: i) to develop/establish analytical methods for identification and quantification of different toxic contaminants; ii) to foster post graduate education plan by giving training; iii) to create awareness among the farmers and food producers; iv) to contribute to the Government/society during policy making by giving scientific data and suggestion to improve food safety and reduce environmental pollution. Post Graduate (PhD, M.Phil and MS) students will develop and validate method under our supervision and collect samples for analysis. New instruments will be purchased and North-‐South and South-‐South collaborations will be strengthened and increased to foster the research activity. Samples will be extracted and cleaned up following the methodologies available or developed if required. The extracted and cleaned up samples will be analyzed by GC, GC-‐MS, HPLC, LC-‐MS. Multiple reaction monitoring (MRM) method will be used for LCMS/MS analysis and selected ion monitoring (SIM) mode will be used for GCMS. Limit of detection, limit of quantification and percentage of recovery will be determined, and quality control and quality assurance will be maintained during each study.

c) Give a summary of the postgraduate education plan Give a maximum half a page summary of the proposed activity with emphasis on the education plan. Please note that this is a summary of Enclosure 1, Section i.

Summary:

The department offers Ph.D., M.Phil., MS degree for 4, 2 and 1 years programme, respectively and students have to submit dissertation to fulfill their degrees. Ph.D and M.Phil students have to attend one year theoretical classes and pass examination. MS students attend theoretical course (38 credit) and research work (10). BS student in their 4th year carry out short project on a specific topic for 2


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credits. Ph.D and M.Phil. students give oral presentation of their research work in front of faculty members and students of the department in the departmental postgraduate seminars. Our plan is to abide by all university and departmental rules and regulation and encourage students to carry out research in the group. Usually, 6 brilliant students either male or female get opportunity to join the group for MS programme each year. Similarly, brilliant BS students (6) also join the group for 4th year short project. Sometimes, both outgoing and incoming students work together in the laboratory. We select PhD students on the basis of their merit, research experience, dedication enthusiasm and ability to work and recommend to the Academic Committee of the Department. All PhD students get opportunity to gain training and attend seminar/workshop to present paper if abstract is accepted. Ph.D and M.Phil. students usually visit abroad as South-‐South collaboration. All students will attend group meeting/seminar/discussion held every two weeks and give presentation according to their slots. Students also visit sampling sites during sample collection and join workshop at rural level.

d) Give a summary of the gender perspective on the research and education plans

Summarize the information given on gender balance as well as strategies and measures to achieve gender balance given in Enclosure 1, section f. Provide your answers under the below headings. NOTE: If you apply for extra gender funds (Enclose 3) you don’t need to fill in this part, just refer to the separate gender application.

Separate gender application to Enclosure 3

Application for Improving Gender Balance

Comment on the current gender balance in perspective of previous years efforts:

Reasons for current gender distribution: Planned strategy to achieve gender balance (if not already the case): Concrete measurements to achieve gender balance:

e) Provide a number of keywords

Keywords: antibiotics, agriculture, environment, food, pesticides, LC-‐MS, organic pollutants


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3. Specifications of costs 2018-‐2020 (Please, carefully read and follow guidelines for Budget, and, if applicable, conditions and instructions for Research Groups’ overhead costs)

3a) Specification of costs in 2018

Specification of Costs in 2018 Costs (SEK) Total (SEK) Equipment/spare parts/service Gas Chromatography Electron Capture Detector (GC-‐ECD) with auto injector

260,000 440,000

Fourier-‐Transform Infrared Spectroscopy (FTIR) 180,000 Consumables/literature/field work Sample Collection 20,000 Pesticide/antibiotic and other standards 20,000 Gases for GC & GC-‐MS 20,000 135,000 Solvent, reagents & chemicals 20,000 Small Apparatus 20,000 Stationary, computer accessories, literature 15,000 Field trip (Interview to farmers, stakeholders, survey etc.) 20,000 Conferences/workshops

a) To be visited Participation at International Pesticide/Environmental/Food Safety Conference/Workshops

50,000 70,000

b) To be arranged National Seminar on food and environment. 20,000 Exchange visits by cooperating scientists Exchange visits by co-‐operating scientists/technician from Sweden or any other EU country

50,000 50,000

Fellowships for training/exchange a) North-‐South

b) South-‐South

One Staff/member to ANFEC Training Programme in Cambodia

10,000 10,000

Support to students Support to students and Research Assistants 80,000 80,000 Activities for improving gender balance Separate application Network administrative costs Audit costs Cost for annual account by external Audit 5000 5,000 TOTAL 790,000

(Insert more rows as needed, or just write below the table)


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3b) Specifications of costs in 2019

Specification of Costs in 2018 Costs (SEK) Total (SEK) Equipment/spare parts/service Total Organic Carbon (TOC) Analyzer 280,000 320,000 LC-‐MS/MS, LC and GC accessories and column 40,000 Consumables/literature/field work Sample Collection 15,000 Pesticide/antibiotic and other standards 5,000 Gases for GC & GC-‐MS 15,000 100,000 Solvent, reagents & chemicals 20,000 Small Apparatus 20,000 Stationary, computer accessories, literature 15,000 Field trip (Interview to farmers, stakeholders, survey etc.) 10,000 Conferences/workshops

a) To be visited Participation at International Pesticide/Environmental/Food Safety Conference/Workshops

50,000 60,000

b) To be arranged National Seminar on food and environment. 10,000 Exchange visits by cooperating scientists Exchange visits by co-‐operating scientists/technician from Sweden or any other EU country

50,000 50,000

Fellowships for training/exchange c) North-‐South

d) South-‐South 70,000 80,000 One Staff/member to ANFEC Training Programme 10,000 Support to students Support to students and Research Assistants 80,000 80,000 Activities for improving gender balance Separate application Network administrative costs Audit costs Cost for annual account by external Audit 5,000 5,000 TOTAL 695,000



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3c) Specifications of costs in 2020

Specification of Costs in 2018 Costs (SEK) Total (SEK) Equipment/spare parts/service High-‐performance liquid chromatography (HPLC) with Auto sampler and PDA detector

300,000 300,000

Consumables/literature/field work Sample Collection 20,000 Pesticide/antibiotic and other standards 20,000 Gases for GC & GC-‐MS 20,000 135,000 Solvent, reagents & chemicals 20,000 Small Apparatus 20,000 Stationary, computer accessories, literature 15,000 Field trip (Interview to farmers, stakeholders, survey etc.) 20,000 Conferences/workshops

e) To be visited Participation at International Pesticide/Environmental/Food Safety Conference/Workshops

50,000

f) To be arranged National Seminar on food and environment. 20,000 70,000

Exchange visits by cooperating scientists Exchange visits by co-‐operating scientists/technician from Sweden or any other EU country

50,000 50,000

Fellowships for training/exchange g) North-‐South h) South-‐South 70,000 80,000

One Staff/member to ANFEC Training Programme 10,000 Support to students Support to students and Research Assistants 80,000 80,000 Activities for improving gender balance Separate application Network administrative costs Audit costs Cost for annual account by external Audit 5,000 5,000 Reference group meeting participation costs 15,000 15,000 TOTAL 735,000



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3d. Justification of budget items

For each budget item, please write a motivation to justify the need and the cost.

Please find elaborate justification in Enclosure 1 (i). Summary is below:

Exsiting GC often gives trouble and becomes out of order. It has been essential for the group to buy a new GC-‐ECD in order to carry out Research work of PhD, MPhil and MS stduents. Therefore, SEK 260,000 is requested to buy a GC-‐ECD with auto injector in 2018 so that organohalogen and organophophorus compounds can be analyzed.

FT-‐IR is very helpful for determining functional groups and thus, identify constituents. The BAN: 04 extended work to study of different constituents in food matrix and also microplastics. Group members of BAN: 04 were trying to buy FT-‐IR for many years but it has not been done. So SEK 180,000 is reserved in the budget of 2018 for FT-‐IR.

We are carrying out research on study of water quality parameter and it has been essential to buy Total Organic Carbon (TOC) Analyzer. TOC is an indirect measurement of organic molecules impurities present in water. SEK 280,000 is requested to buy TOC analyzer in 2019.

Efficiency of old HPLC has been reduced. But research work on different contaminants like aflatoxins, antibiotics, additives have beed increased to analyse by HPLC. In order to get more accurate and precised results SEK 300,000 is requested to purchase HPLC with PDA detector and auto sampler in 2020.

In order to meet the expenses of transportation, buying sample collection kits, incentive to the collectors and other relevant expenses SEK 20,000, 15,000 and 20,000 have been requested in the budget of 2018, 2019 and 2020, respectively.

New standard of pesticides and antibiotics will be requied to modify existing methids and develop new methods and need to be purchased. Therefore, SEK 20,000 in 2018, 5,000 in 2019 and 20,000 in 2020 have been requested in the budget. SEK 20,000 in 2018, 15,000 in 2019 and 20,000 in 2020 have been requested in the budget to purchase gases for GC, GCMS and LCMS/MS.

SEK 20,000 is requested for each year in the budget of 2018-‐2020 to purchase solvents, reagents and chemcials. To buy small apparatus SEK 20,000 is requested for each year in the budget of 2018-‐2020. SEK 15,000 is requested for each year in the budget of 2018-‐2020 for stationery purposes.

SEK 20,000, 10,000 and 20,000 are requested in 2018, 2019 and 2020 to meet the costs of field trips including interview to farmers, stakeholders and survey.

It would be a good opportunity for us to attend any of the event, and represent BAN:04 and meet scientists around the world and look for future collaboration. So, 50,000 SEK is requested for each year for participation at the Conference. It is planned to organize national seminar each year during 2018-‐2020 and SEK 20,000 have been requested for 2018 and 2020, and SEK 10,000 is requested for 2019 for National Seminar on Food and Environment.

The BAN:04 group hope that Swedish scientists and scientists from other countries will visit BAN:04 research laboratory during 2018-‐2020. SEK 50,000 is requested for each year for exchange visit by cooperating scientists. SEK 10,000 is requested for each year in 2019 and 2020 for ANFEC training. To


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support postgraduate students, research assistants and technician SEK 80,000 is requested for each year in the budget of 2018-‐2020. For audit cost SEK 15,000 is requested in the budget of 2018-‐2020. And for participation in the Reference Group Meeting in 2020 SEK 15,000 is requested in the budget of 2020.

4. Staff and students in the group/network

List staff and students planned to be actively involved in the proposed activity.

a) List staff in the group/network Staff members who also are active students are to be listed ONLY under students. Networks list members of Board on Management Committee, node coordinators etc. Note that support staff (Supp.) comprises all non-‐academic and undergraduate staff engaged, e.g. laboratory assistants, technicians, secretaries, etc.

Gender F/M

Given name, family name Position held / Function1

Staff qualifications PhD Other

Ac. Supp.

F Nilufar Nahar (Advisor) Professor √ M Mohammad Shoeb (Leader) Professor √ M Md. Abdul Aziz Professor √ F Abida Sultana Assistant Professor √ M Md. Kamrul Hasan Assistant Professor √ M Md. Akram Hossain Technical Officer √ M Kazi Isteag Lab Assistant √ M Md. Mizanur Rahman Lab Assistant √ F Sanjida Farid Lab Assistant √


b) List students in the group/network (If already known) Students to be included should be those that benefit directly of the ISP support (fellowships) or indirectly by using consumables and equipment provided through the ISP support.

For each student, provide the following information: 1) Gender (female/male; F/M) 2) Full name 3) Target degree (PhD/MPhil/MSc) 4) Starting year on current degree study 5) Tentative title of thesis / subject 6) Expected year of graduation 7) Whether staff member or not 8) a) Local (L) or Sandwich (S) student; b) Direct or Indirect benefit of ISP support (D/I)

1 Indicate deputy leader/coordinator, if applicable


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1 2 3 4 5 6 7 8a 8b F/M Name Target

Degree Start year

Thesis/Subject Grad year

Staff (Y/N)

L/S D/I

M

Md. Shahed Reza Ph.D. 2017 Studies of contaminants (multi pesticide residues) in fruits and vegetable

2021

No Local

Indirect support

F Rafiza Islam Ph.D. 2017 Investigation of Antibiotic Residues in Meat, Milk and Egg Samples

2020

No Local

Direct support

M Md. Mizanur Rahman

Ph.D. 2017 Isolation and Structure Elucidation of Secondary Metabolites from Three Medicinal Plants

2020

No Local

Indirect support

F Farhana Sobnom M.Phil. 2017 Level of organophosphorus insecticides in the diet and blood samples of an adult rural population in relation to their cardiovascular risk

2019

No Local

Indirect support

M Saiful Islam Bhuyian

MS 2017 Method development and validation for determination of Chloramphenicol in Shrimp and Prawn samples by HPLC.

2018

No Local

Indirect support

M Md. Mafizul Islam MS 2017 Analysis of Artificial Preservatives, Sweetening and Stimulating agents in Carbonated Beverages by HPLC

2018

No Local

Indirect support

M Tofael Ahmed MS 2017 Title: Bioactive compounds from Morus alba (Tut)

2018

No Local

Indirect support

F Farhana Sharmin MS 2017 Chemical contaminants in the aquatic environment of the Southern part of Bangladesh

2018

No Local

Indirect support

F Salma Akter Mou MS 2017 Analysis of Sulfadrugs and Chloramphenicol in Poultry Meat and Beef Samples.

2018

No Local

Direct support

M Murshid Hossain MS 2017 Method development and validation for the analysis of artificial sweeteners and preservatives in fruit juice using HPLC.

2018

No Local

Indirect support


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F Shatabdi Roy BS, MS 2016, 2017

Analysis of food dye in commercial sweetmeat

2017,2019

No Local

Indirect support

M Md.Ahsan Habib Khandakar

BS, MS 2016, 2017

Analysis of colouring pigments in watermelon (Japanese variety)

2017,2019

No Local

Indirect support

M Tanhaul Islam BS, MS 2016, 2017

Analysis of colouring pigments in watermelon (Local variety)

2017,2019

No Local

Indirect support

M Forkan Saroar

BS, MS 2016, 2017

Analysis of food dye in commercial sweets

2017,2019

No Local

Indirect support

M Elias Ahmed BS, MS 2016, 2017

Isolation of bioactive compounds from Morus alba (tut) leave

2017,2019

No Local

Indirect support

M Tauhidur Rahman

BS, MS 2016, 2017

Isolation of bioactive compounds from Morus alba (tut) leave (non polar part)

2017,2019

No Local

Indirect support

M Rakibul Islam BS 2017 Study of chemical pollutants in drinking water

2018

No Local

Indirect support

M Md. Hossain Sohid Shoroardy

BS 2017 Study of water quality parameter of the Turag river

2018

No Local

Indirect support

M Md.Reazul Alam Rifat

BS 2017 Analysis of additives in processed food

2018

No Local

Indirect support

M Md. Farhanul Alam

BS 2017 Analysis of additives in processed food

2018

No Local

Indirect support

M Palash Chandra Nandi

BS 2017 Bioactive compounds from natural products

2018

No Local

Indirect support

F Khandoker Tahmina tasnim

BS 2017 Chemical contaminants in food

2018

No Local

Indirect support

F Sunjida Akter BS 2017 Chemical contaminants in food

2018

No Local

Indirect


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support

M Uzzal Shaha BS 2017 Bioactive compounds from natural products

2018

No Local

Indirect support

5. Scientific contacts/cooperation

Indicate which contacts with scientists working in the field of the proposed activities are established or planned (at your own university/institute, nationally, regionally, or internationally). The present and expected benefits of these contacts should be further described in the Research description (Enclosure 1).

a) List already established scientific contacts/collaboration that were engaged in active collaboration with your research group/network in 2015-2017.

For each collaborator, provide the following: a) Gender (female/male; F/M) b) Title c) Full name d) Affiliation e) Country

Gender F/M

Title Name Affiliation Country

M Professor Henrik Kylin Dept. of Thematic Studies, Environmental Changes, Linköping University

Sweden

M Professor Jae-‐ Han Shim Chonnam National University Republic of Korea

F Dr Kesiny Phomkeona

Department of Chemistry, National University of Laos

Lao PDR

M Professor A.M. Abd El-‐Aty

Depatment of Veterinary, College of Veterinary Medicine, Konkuk University, Seoul

Republic of Korea

M Mr Heng Savoeun Department of Chemistry, The Royal University of Phnom Penh

Cambodia

M Dr Syed Nurul Alam

Head (CSO), Entomology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur, Dhaka

Bangladesh

M Dr. Volkan Degirmenci

School of Engineering, University of Warwick

UK

F Professor M. Niamul Naser

Department of Zoology, University of Dhaka

Bangladesh

F Dr Tahmina Akter Department of Entomology, Faculty of Agriculture, Sher-‐e-‐Bangla Agricultural University, Dhaka

Bangladesh


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a) List scientific contacts for collaboration you intend to establish with your research group/network in 2018-‐2020. For each collaborator, provide the following: 1) Gender (female/male; F/M) 2) Title 3) Full name 4) Affiliation 5) Country

Gender F/M

Title Name Affiliation Country

Female Dr. Proum Sorya Lecturer, Chemistry Department, Royal University of Phnom Penh

Kingdom of Cambodia

Male Professor Barry Noller Centre for Mined Land Rehabilitation, Sustainable Minerals Institute , The University of Queensland, Brisbane

Australia

Male Deputy Coordinator

Stewart Jones APFAN (Asia Pacific Food Analysis Network)

Australia

Female Dr. Christine O'Connor

Assistant Head, School of Food Science and Environmental Health, Dublin Institute of Technology

Ireland

Male Dr. Farhan Khan Associate Professor, Department of Science and Environment, RUC Roskilde University

Denmark

Female Dr. Cecilia Berg Associate Professor, Department of Environmental Toxicology, Uppsala University

Sweden

Male Professor Marcel Jaspars Department of Chemistry, University of Aberdeen

UK

Male Professor Kazi Matin U Ahmed

Department of Geology Faculty of Earth and Environmental Sciences University of Dhaka

Bangladesh


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6. Other funding received (besides from ISP) 2015-‐2017, and available and foreseen funding for 2018-‐2020

Specify other funding obtained in 2015-‐2017, and other foreseen sources of funding for 2018-‐2020, from national as well as from other sources. List each source on a separate row, and give the amount for each year on separate rows. Estimate the amount in USD (currency conversion can be done at www.xe.com).

a) Other funding (besides from ISP) obtained in 2015-‐2017 Source Grant period/

mmyy-‐mmyy Total Grant, USD

HEQEP Project, University Grants Commission (UGC) of Bangladesh

Jan 2015-‐August 2017 406,250

University of Dhaka Jan 2015-‐Dec 2017 20,000 Ministry of Education, Government of Bangladesh Jan 2015-‐June 2018 35,000 Ministry of Science and Technology, Govt of Bangladesh July 2015-‐June 2016 3,750 Food and Agricultural Organization (FAO) Jan 2015-‐Dec 2017 3,000 Small project from UGC Jan 2015-‐Dec 2017 3,750


b) Other available or foreseen funding for 2018-‐2020 Source Grant period/

mmyy-‐mmyy Total Grant, USD

Ministry of Science and Technology, Government of Bangladesh

Jan2018-‐June 2020 15,000

University Grants Commission (UGC) of Bangladesh Jan 2018-‐Dec 2020 12,500 University of Dhaka Jan 2018-‐Dec 2020 45,000 Bose Centre for Advanced Research, Science Faculty, DU Jan 2018-‐Dec 2020 5,000



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7. Main equipment available

a) List relevant facilities and infrastructure available to the group. For equipment, give type and name of the manufacture of main items and other articles. Start with your most important equipment. Please, note if the equipment is not functioning properly and why.

Equipment type, model, and name of manufacturer Year acquired Fully functional, yes/no If no, indicate why

Liquid chromatography mass spectrometry (LC-‐MS/MS, Shimadzu LCMS-‐8050)

2015 Functional

Gas Chromatograph with ECD detector (GC-‐ECD, Shimadzu 2010)

2008 Functional

Gas Chromatograph with FID detector (GC-‐FID, Shimadzu 2025) 2011 Functional

Gas Chromatograph with ECD detector (GC-‐ECD, Shimadzu 17A) 2001 Functional

Gas Chromatograph-‐Mass Spectrometer (GC-‐MS; Agilent 6890) 2008 Functional

High Performance Liquid Chromatography (HPLC-‐PDA and RF-‐20A Prominence Fluorescence Detector; Shimadzu CTO 10 ASVP)

2013 Functional

High Performance Liquid Chromatography (HPLC-‐PDA; Shimadzu 10 VP) 2001 Functional

UV-‐Visible spectrophotometer (UV-‐1800, Shimadzu) 2013 Functional

Rotary Evaporator, Heidolph, Germany 2010, 2012, 16 Functional

Mechanical Shaker (IS-‐971R) with temperature controlling system 2010 Functional

Recipro Shaker (RS-‐1) 2010 Functional Portable pH Meter 2017 Functional Centrifuge machine [Sigma, Model-‐2-‐16 P, Heraeus Sepatech (Labofuge A)] 2015 Functional

Ultrasonic bath (Powersonic 610/Hwashin technology, Korea) 2015 Functional

Analytical balance (Mettler Toledo AL 104 and Shimadzu ATY124) 2014, 2016 Functional

Oven [Salvis (G-‐1020), Salvis (G-‐1079), Carbolite] Different year Functional Water purification system (BOECO, Germany) 2014 Functional Power Generator 50KVA 2016 Functional Oven (JSOF-‐250, Korea) 2017 Functional Oven (Binder, ED 115, Germany) 2017 Functional Shaking JSSI-‐100T, Korea 2017 Functional Magnetic stirrer with hotplate, HSD 180, Korea 2017 Functional



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b) List below main equipment needed in the near future, with justification. Specify to the degree possible.

Equipment Justification High performance liquid chromatography (HPLC) with Auto sample and PDA detector

For analytical and preparative purpose of organic compounds (antibiotics, aflatoxins, additives).

Gas chromatography Electron Capture Detector (GC-‐ECD) with auto injector

For organohalogen and organophophorus compounds analysis.

Fourier-‐Transform Infrared Spectroscopy (FTIR) For determining functional groups and thus, identify constituents

Total Organic Carbon (TOC) Analyzer For direct measurement of organic molecules impurities present in water

8. Environmental impact

Information is required about measures taken to reduce environmental impacts following the Environmental Impact Assessment for ISP as submitted to Sida in August 2009 (available on request).

a) Indicate which of the following measures your group/network has already implemented to reduce negative environmental impact.

Does your group/organization: Yes No

A strategy to reduce negative environmental impact caused by travelling and transportation?

√

The use e-‐meeting techniques? √ A strategy to reduce the use of electric power? √ Considering environment impact criteria in procurement? √ Practicing sorting of waste categories for recycling? √ A system for scrapping decommissioned equipment? √ A management system for chemical and hazardous waste? √ Internal discussion of how any negative environmental impact of your activities can be reduced?

√

Engagement in external activities – in research, dissemination and/or society outreach – on how negative environmental impacts may be reduced?

√

b) Comment on the no-‐answers in the previous question, if any.

What are the reasons why some measures have not been implemented? Are there plans to do it in the future? Are there structural obstacles, such as regulations or lack of regulation/technology, etc.? Also comment on the practicing of measures in general to reduce negative environmental impact.


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9. Summary of results for previous agreement period

Provide a summary of the progress so far in the current agreement period (2015-‐2017), or latest years for new applicants.

1) Give a summary of the major achievements in the period, including results of scientific research activities. Place the achievements and results in relation to the objectives in the original proposal (if applicable). Not only major changes are interesting, but also small changes that may lead to larger changes over time.

Abida Sultana and Farzana Saleh got PhD degrees. Zerin Sultana Munia defended her PhD thesis and the committee recommended for degree. Farzana Khalil and Tonima Mustafa submitted their PhD theses and Md. Anower Hossain submitted his MPhil thesis. Mahfuz will submit soon. All PhD will be awarded in 2017. A total of 15 MS students and 16 4th Year BS (Honors) students completed their research work, submitted theses/projects and got relevant degrees.

Two male and one female students enrolled for PhD and one female student enrolled for MPhil program. One of the Ph.D. researcher received Fellowship from University Grants Commission (UGC) of Bangladesh and several MS students also received Fellowship from Ministry of Science and Technology, Government of Bangladesh.

A grant of 406250 USD was received on project Chemical Contaminants on Food and their effects on human health from UGC. LCMS/MS was purchased from the project and it is being routinely used. Fume hoods renovated, laboratory equipments’ room rearranged and refurbished. Post graduate students room renovated with new table, chair, book shelf and WIFI were got from the project also. Centrifuge machine, freeze drye, water purification system, rotavapor, Oven, pH meter and shaker were procured and installed. A research project for analysis of residual pesticides in vegetable samples from Bangladesh Food Safety Laboratory Network (BFSLN) coordinated by Food and Agricultural Organization (FAO) was completed and reports were submitted and finally, accepted by FAO authority. This activity increased the strength and reputation of the group.

Contribution of BAN:04 for ANFEC is continued. BAN:04 organized ANFEC-‐NITUB Regional Workshop on Gas Chromatography, Quantification and Quality Assurance in 2016. Nilufar and Shoeb attended ANFEC Board member meetings in Cambodia and Laos in 2015 and 2017, respectively. Rafiza Islam, a PhD student attended ANFEC workshop in Laos in 2017. BAN:04 keeps always contact with Laos and Cambodia for ANFEC activity.

One MS student received best poster award in 16 Asian Chemical Congress (ACC) and two MS students received travel grant Award to attend SETAC Asia/Pacific Conference 2016 in Singapore. Two students received DEAN’s award for their outstanding performances in undergraduate level.

Twenty papers were published in peer reviewed journals. Fifty three papers in conference/workshop have been published/presented.

In order to create awareness of toxic effects of chemical contaminants and disseminate knowledge one International Seminar and one National Seminar on Chemical Contaminants in Food, and three


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Workshops with Farmers at rural level (Mymensign, Nuritola, Comilla and Nurundi, Jamlapur) were organized. BAN:04 also organized several training program on GC, HPLC and LCMS/MS to train local researchers.

Existing local and international collaborations were strengthened significantly by visit and electronic communications. Nilufar, Shoeb and Kamrul visited collaborators Prof Shim and Prof Ety laboratories in South Korea and they also visited BAN:04 group. Farzana, a female PhD student visited CNU, South Korea for six months. Several other visitors also came to BAN:04 group. Shoeb visited University of Warwick, UK and a new collaboration was established with staffs of Faculty of Engineering Environment, University of Warwick, UK.

An easy, quick and cheap method has been developed to identify the red dye in watermelon. Shimadzu protocol was modified and validated to analyze six sulfa drugs i.e., sulfadiazine, sulfadimethoxine, sulfamethazine, sulfamerazine, sulfamethiazole and sulfamethoxypyridiazine in chicken meat and beef samples by LC-‐MS/MS coupled with ESI and TQ mass analyzer. Reported methods for the analysis of aflatoxin (B1, B2, G1, G2) was validated to analyze them in rice samples. Dissipation patterns of organophosphate pesticides in selected vegetable samples were evaluated. Concentrations of DDTs in twenty two fresh water fish samples were determined. Organochlorine compounds, heavy metals and fatty acid compostion in ninteen marine fish samples were being carried out. Please see students’ abstracts for more details. Also include comments and analysis of:

-‐ How far your group has come in fulfillment of the stated objectives. :The group is recognized nationally as one of the laboratories of the country to analyze organic pollutants in food and environment and also contributed to the region (Laos and Cambodia) and known to International community. Objectives have been partly fulfilled in terms of instruments and human resources. But still need to contribute to the national level for improving food safety and quality for better health and clean environment; hence can be accounted for little more than 50%.

-‐ Whether the results achieved were in line with expected/planned outcomes. Motivate your answer.

: Yes, the results obtained are in line with expectation or planed. Students got their respective degrees. Research grant obtained from HEQEP, UGC and project from Ministry of Science and Technology, GOB and from Ministry of Educations have successfully been completed. Main equipment is functioning well and research work based on the equipments are going well.

-‐ Why, or why not, results have been achieved. Comment on the (limiting or favorable) conditions for reaching the results.

: Three female students had family problem and submission of theses was delayed. Thus hindered publishing their research results in the peer reviewed journals.

-‐ What needs to be adjusted in order to increase the likelihood to reach the objectives.


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Still, we need to go a long way for sustainable development. We are working for food safety and better environment, and contributing to the society. Food safety is burning issues in Bangladesh at present and we are helping the government for it. We need to develop methodology for analysis of many contaminants including pesticides, antibiotic, ripening agents in food matrices and environment. Hence skill manpower needs to be produced and laboratory needs to be improved. Fellowship for researchers, modern sophisticated equipment’s and consumables are required for these purpose.

2) Give a summary of the staff structure, students active and degrees awarding, dissemination, meetings, outreach, and visits to and from your group/network (scientific exchange) for the previous granting period.

Staff PhD or equivalent Other academic Technicians Female Male Female Male Female Male

Number of staff 2017 2 3 0 2 1 6

Students/degrees PhD or equivalent MSc/Mphil/Lic. BSc thesis students Sandw Local Sandw Local

F M F M F M F M F M Number of students 2017 1 2 2 4 2 6 Number of degrees 2015-‐2017 5 1 5 11 4 12

Specification of awarded degrees 2015-‐June 2017 Gender, First name, Family name, Degree, Title of thesis (full abstract in Enclosure 6) 1) F, Abida, Sultana, PhD, Chemical Contaminants in Rice, Spice and Vegetable Samples. 2) F, Farzana, Saleh, PhD, Health Effects of Isoflavones from Bangladeshi Soymilk in Postmenopausal

Women. 3) F, Zerin Sultana Munia, PhD, Studies of Chemical contaminants in different food staff 4) F, Tonima Mustafa, PhD, Organohalogen Residues of Fishes from Different Trophic Levels. 5) F, Farzana Khalil, PhD, Dichlorodiphenyltrichloroethane in Environmental Samples and Human Blood

of Chittagong Chemical Complex Area and Pesticide Residues in Some Vegetable Samples. 6) M, Anowar Hosen, MPhil, Chemical and biological studies of leaves and twigs of Anogeissus rivularis

and some hypoglycemic herbal formulations. (Summited thesis and graduation will be in 2017) 7) M, A. B. M. Mahfuzul Alam, PhD, Method development for quantification of some non-‐pharmacopeial

pharmaceutical drugs (due to submit and finish by 2017). 8) M, Shaurav Sarker, MS, Studies of polyaromatic hydrocarbons in downstream water samples from

Buriganga, Meghna, Turag rivers and Hatirjheel lake. 9) M, Md. Sharear Saon, MS, Evaluation of locally produced Vitamin B1, B2, and B6 by UV-‐VIS

spectrophotometric. 10) M, Md. Nazimuddin, MS, Studies of Polyaromatic Hydrocarbons in Upstream Water Samples from

Buriganga, Meghna, Turag rivers and Gulshan Lake. 11) M, Prithu Mondal, MS, Chemical and Biological Activity Studies of Mushrooms. 12) F, Sharmin Sultana, MS, Assessments of chemical constituent in some pesticide treated and untreated

vegetables. 13) F, Nasrin Jahan, MS, Nutritional and Phytochemical Study of Some Edible Vegetables. 14) M, Md. Amjad Hossain, MS, Organochlorine Pesticides and Fatty Acid Composition in Fresh water Fish

samples. 15) M, Md. Golam Kibria, MS, Fatty acid compositions and bioaccumulation of heavy metals in fishes of

different trophic level. 16) M, Md. Ahasan-‐Ul Hoque Sakib, MS, Investigation of Three Surfactants in Water by

Spectrophotometric Method.


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17) M, Md. Mazharul Islam, MS, Organochlorine Compounds and Arsenic, Cadmium and Lead in Marine Fish Samples of Bangladesh.

18) M, Rabiul Islam, MS, Studies of fatty acid compositions and level of Cr and Hg in marine fish samples. 19) M, Anowar Hossain Khan, MS, Organochlorine Compounds and Heavy Metals in Sediment Samples

from Coastal Area of Bangladesh. 20) F, Jannatun Noor Jeouty, MS, Studies of microplastic, organochlorine compounds and toxic metals in

marine fish samples of Sundarban region. 21) F, Rehnuma Tabassum, MS, Study of Water Quality Parameters and Environmental Contaminants in

the Aquatic Ecosystem of the Bay of Bengal. 22) F, Most. Salma Khatun, MS, Bioactive Secondary Metabolites from Plant Averrhoa bilimbi L. 23) M, Rehnuma Tabassum, BS, Estimation of Anionic Surfactant Sodium Dodecyl Sulphate in water from

Ramna Lake, Hatirjheel Lake and Dharla River of Dhaka city using Spectrophotometric Technique. 24) M, Anowar Hossain Khan, BS, Evaluation of Diclofenac Sodium in Pharmaceutical Preparations by UV-‐

Visible Spectrophotometer. 25) M, Rabiul Islam, BS, Qualitative Estimation of Preservative and Stimulant in soft drink 7UP by UV-‐

Visible spectrophotometer. 26) M, Md. Mazharul Islam, BS, UV-‐Visible Spectrometric studies for Assessment of Sodium Benzoate and

Caffine in Sprite Sample. 27) F, Salma Akter Mou, BS, Studies of Food Preservatives and Color additives in orange Jelly Samples by

UV –visible spectrophotometer. 28) F, Tanzina Akther, BS, Study of physiochemical properties and Analysis of sodium benzoate, caffeine

in jam samples. 29) M, Mafizul Islam, BS, Quantitative estimation of methyl paraben in chilli sauce by UV-‐vis

spectrophotometer. 30) M, Saiful Islam Bhuyian, BS, Studies of physico-‐chemical properties of orange soft drinks and

determination of food additives by UV-‐visible spectrophotometric method.

31) M, Tofael Ahmed, BS, Studies of physiochemical properties of orange juice and determination of yellow 6 by UV-‐visible spectrophotometric method.

32) M, Saiful Islam, BS, Determination of methyl paraben and lycopene in tomato ketchup by UV-‐Visible spectrometry method

33) M, Md. Ahsan Habib Khandakar, BS (Graduation-‐2017), Studies of Presence of Allura Red (Red-‐40) in Watermelon by UV-‐Visible Spectrophotometry

34) Tanhaul Islam, BS, Studies of Presence of Erythrosine (Red 3) in Watermelon by UV –Visible Spectrophotometry

35) Shatabdi Roy, BS, Studies of Synthetic Color Additive in Laddu 36) Forkan Saroar, BS, Studies of Synthetic Colorants in Sweet (Laddu) 37) Elias Ahmed, Phytochemical investigation of Morus alba L. 38) Tauhidur Rahman, Isolation of Secondary metabolites from the DCM extract of the plant Morus alba

L. (Insert more rows as needed, or just write below the table) Publications in scientific journals 2015-‐June 2017

Author(s) (Year) Title. Journal, ISSN-‐number, Vol., Pages, DOI2 Indicate how large a part of the work was performed at home University (<25%, 25-‐50%, >50%)

Was ISP support acknowledged (Yes/No) (Not filled by new applicants)

S. Mandal, N. Khuda, M R Mian, M Moniruzzaman, N Nahar, M I R Mamun and M Shoeb (2015), Analysis of Ground and Surface Water Samples from some area of Dhaka city for Polycyclic Aromatic Hydrocarbons (PAHs),

Yes

2 Digital Object Identifier (www.doi.org) – to be provided if available


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Dhaka University Journal of Science, ISSN:1022-‐2502, 63(1): 59-‐60. (>50%) M.N.U. Al Mahmud, F. Khalil, M.M. Rahman, M.I.R. Mamun, M. Shoeb, A.M. Abd El-‐Aty, Jong-‐Hyouk Park, and Ho-‐Chul Shin, (2015), Analysis of DDT and its metabolites in soil and water samples obtained in the vicinity of a closed-‐down factory in Bangladesh using various extraction methods, Environ Monit Assess, ISSN: 0167-‐6369, 187:743. DOI: 10.1007/s10661-‐015-‐4965-‐9. (25-‐50%)

Yes

N. Nahar, H. M. Sharma, M. Shoeb, M. I. R. Mamun and M. L. Sharker (2015), Dissipation Pattern and Residue of Fenvalerate in Tea of Bangladesh, Dhaka University Journal of Science, ISSN 1022-‐2502, 63(2):73-‐76. (50>%)

Yes

M. Shoeb, M. S. U. Khandaker, and N. Nahar (2015), Oxygenated Secondary metabolites from Endophytic Fungi Isolated From Ocimum sanctum, Journal of Basic & Applied Sciences, ISSN: 1927-‐5129, 11, 434-‐439. (50>%)

Yes

Sultana A, Hossain M R., Mamun M I R, Shoeb M, and Nahar N. (2015) Compositions of Rice Bran Oil in some selected Bangladeshi paddy samples. Journal of Bangladesh Chemical Society, ISSN 1022-‐016X, Vol.27 (1&2) , 33-‐37, (50>%)

Yes

M. Shoeb, A. Mahim, M. I. R. Mamun and N. Nahar (2016). Organochlorine pesticide residues in poultry meats of Bangladesh. Croat. J. Food Sci. Technol, ISSN: 1847-‐3466, 8(1): 30-‐33. DOI: 10.17508/CJFST.2016.8.1.04 (>50%)

Yes

M. A. Hossain, M. Shoeb and N. Nahar (2016). DDT and Its Metabolites in Fresh Water Fish Samples. Journal of Food Science and Engineering, ISSN:2159-‐5828,6: 344-‐350. DOI: 10.17265/2159-‐5828/2016.06.006 (>50%)

Yes

N. Nahar, W. I. Khan and M. Shoeb (2016). Physico-‐Chemical Analysis and Composition of Camel Milk of Bangladesh. Journal of Basic & Applied Sciences, ISSN: 1927-‐5129, 12: 231-‐235. (>50%)

Yes

A. Sultana, R. Islam, M. M. Islam, M. Shoeb and N. Nahar (2016). Study of Preservatives and Stimulants in Commercial Soft Drinks. Bangladesh Pharmaceutical Journal, ISSN: 0301-‐4606, 19(1): 89-‐95. (>50%)

Yes

Z. S. Munia, M. Shoeb, M. Kamruzzaman, M. I. R. Mamun and N. Nahar (2016). Determination of the Toxicity Level of Imidacloprid in Rice sample Produced in Bangladesh. J Bang. Chem. Soc, ISSN 1022-‐016X, 28 (1 & 2): 1-‐4. (>50%)

Yes

A.Sultana, M. A. H.Sakib, M. G. Kibria, M. R. Islam, M. Shoeb and N. Nahar (2016). An easy and cheaper method to assay paracetamol and metronidazole. J. Bang. Chem. Soc, ISSN 1022-‐016X, 28 (1 & 2): 5-‐11. (>50%)

Yes

M. Shoeb, M. Khondker, and N. Nahar (2016). A new steroidal glycoside from Corypha taliera Roxb. a globally endangered species. Nat Prod Res, ISSN : 1478-‐6419, 30(3): 281-‐5. (>50%)

No

Abida Sultana, Md. Sharear Saon, Mohammad Shoeb and Nilufar Nahar (2016). Evaluation of Vitamin B1, B2 and B6 Tablets in Bangladesh by UV-‐Vis Spectrophotometer, Indian Journal of Pharmaceutical and Biological Research, ISSN: 2320-‐9267, 4 (4), 32-‐38. (>50%)

Yes


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Khan S H, Matin M, Sultana N, Mosihuzzaman M, Ali, L Rokeya, B.. Khan A K A, Shoeb M. and Nahar N (2016). Hypoglycemic activity of Scoparia dulcis L. extract and isolation of flavonoid glycosides, J Bang. Chem. Soc, ISSN 1022-‐016X, 28 (1 &2) 1-‐4, (>50%)

Yes

M K Hasan, M A Hossain, A Sultana, M Shoeb and N Nahar (2017) Evaluation of Diclofenac by UV-‐Vis Spectrophotometer in Some Locally Produced Tablets. Dhaka University Journal of Science, ISSN:1022-‐2502, 65(2), 163-‐165, (>50%)

Yes

Mohammad Shoeb, Prithu Mondal, Henrik Kylin and Nilufar Nahar (2017). Chemical and Biological Activity of Mushrooms Naturally Occurring in Bangladesh, The Asian Journal of Pharmacognosy, eISSN: 0128-‐1119 1(3):42-‐51, (>50%)

Yes

Shoeb M, Al Mamun M S, Noor R E, Al Mahmud M I R, and Nahar (2017). Residual DDTs and fatty acid compositions in popular two fish samples, Dhaka Univ. J. of Sci., ISSN:1022-‐2502, 65 (1) 77-‐80. (>50%)

Yes

Mohammad Shoeb, Md. Amjad Hossain, Md. Golam Kibria, Tonima Mustafa and Nilufar Nahar (2017), Bioaccumulation of Mercury in Fish Species from Different Trophic Level, Journal of Food Science and Engineering, ISSN:2159-‐5828, 7, 363-‐367, (>50%)

N. Nahar, M. M. Hossain, M. N. U. A. Mahmud, M. Shoeb, G. A. Latifa and K. H. Kabir (2016). Dissipation of Cypermethrin in Bean and Cauliflower. Dhaka Univ. J. Sci., ISSN:1022-‐2502, 64(1): 89-‐90. (>50%)

Yes

Mou S A, Akther T, Shoeb M, Sultana A and Nahar N (2017). Studies of preservative and coloring agent in jam and jelly, Dhaka Univ. J. of Sci., ISSN:1022-‐2502, 65 (1) 83-‐84. (>50%)

Yes


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List all contributions by your group to conferences/workshops/courses/meetings 2015-June 2017, including invited lectures at external institutions/organizations Presenter, Co-authors, Title of the presentation, Name of event, Venue, Date

Form: oral/poster

Invited yes/no

F. Khalil, M. Shoeb, MIR. Mamun, N. Nahar, Organochlorine pesticides in human blood samples of people living in a close down factory of Chittagong Chemical Complex area 45th IUPAC World Chemistry Congress, 9-14 August 2015, Busan, South Korea

Poster No

M. Shoeb, Halogenated organic pollutants in food and human blood samples of Bangladesh, Group Seminar, Department of Thematic Studies-Environmental Changes, Linkoping University, Sweden, 31 August 2015.

Oral No

N. Nahar, Evaluation of herbal medicines by chromatographic and spectroscopic methods, CHEMRAWN XX, 20th IUPAC Conference On Chemical Research Applied To World Needs, 06-09 November 2015, Dhaka, Bangladesh

Oral Yes

M. Shoeb, N. Nahar, Bioactive secondary metabolites from endophytic fungi of Bangladesh, CHEMRAWN XX, 20th IUPAC Conference On Chemical Research Applied to World Needs, 06-09 November 2015, Dhaka, Bangladesh

Oral No

M. Shoeb, N. Nahar, Bioactive secondary metabolites from antidiabetic plant materials and endophytic fungi, 8th Asian Network of Research on Antidiabetic Plants (ANRAP), 23-25 November 2015, Malaysia

Oral Yes

M. Shoeb, N Nahar, Halogen specific detector for analysis of chlorinated compounds, 2nd International Bose Conference-2015, 3-4 December 2015, Dhaka, Bangladesh

Oral No

M. Shoeb, P. Mondal, N. Nahar, Chemical and biological activity studies of naturally occurring mushroom, 2nd International Bose Conference-2015, 3-4 December 2015, Dhaka, Bangladesh

Oral No

M. Shoeb, Characterization of drinking water disinfection by-product, 6th Regional Public Health Conference , 20-21 December 2015, Dhaka, Bangladesh

Oral No

M. Shoeb, N. Nahar, Fungal endophytes as potential sources of bioactive natural products, Microbiology International Conference-2015 & 29th Annual General Meeting, 26-28 December 2015, Dhaka, Bangladesh

Oral No

M. Shoeb, N. Nahar, Monitoring of endocrine disruptor in the environment, Diabetes and Endocrine Conference. Journal, 17-18, December 2015, Dhaka, Bangladesh

Abstract published but excused due to sickness

N. Nahar, Pesticides and their effects on human health., 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Oral Yes

M. Shoeb and N. Nahar, Monitoring of Endocrine Disruptor in The Environment, 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Oral Yes


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M. Shoeb and N. Nahar, Bioaccumulation of Arsenic and Other Metals in The Biota of The Contaminated River Meghna. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Oral Yes

M. Shoeb, N. Nahar, Chemical contaminants in food stuff of Bangladesh. International Seminar on Chemical Contaminants in Food, 20 March 2016, University of Dhaka, Dhaka, Bangladesh

Oral Yes

M. Shoeb, P. Mondal and N. Nahar, Chemical and Biological Activity Studies of Natural Occurring Mushroom. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

Z. S. Munia, M. Kamruzzaman, M. Shoeb, M. I. R. Mamun and N. Nahar, Residual Imidacloprid and its Toxicity Level in Rice Samples of Bangladesh . 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Oral No

T. Mustafa, M. N. Naser, G. A. Latifa, M. Shoeb and N. Nahar, Organohalogen Pesticide Residues of Fishes From Different Trophic Levels of Meghna River. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Oral No

R. Tabassum, M. R. Islam, M. Shoeb and N. Nahar, Anionic Surfactant in Water Samples from Two Lakes and One River of Dhaka City. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

W. I. Khan, M. Shoeb and N. Nahar, Physico-chemical Analysis and Composition of Camel Milk of Bangladesh. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

R. Islam, M. M. Islam, A. Sultana, M. Shoeb and N. Nahar, Quantitative Estimation of Sodium Benzoate and Caffeine in Popular Soft Drinks. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

A. H. Khan, A. Sultana, M. Shoeb and N. Nahar, Evaluation of Diclofenac Sodium in Pharmaceutical Products by UV-Visible Spectrophotometer.16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

S. Sultana, N. Jahan, M. Shoeb and N. Nahar, Assessment of Chemical Constituent of Pesticides Treated and Untreated Vegetable.16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

M. A. Hossain, M. G. Kibria, M. Shoeb and N. Nahar, Organochlorine Pesticides Residues and Fatty Acid Composition in Fresh Water Fish Sample . 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

M. G. Kibria, M. A. Hossain, M. Shoeb and N. Nahar, Bio-accumulation of Mercury in Fish from Different Tropic Level.16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

N. Jahan, S. Sultana, M. Shoeb and N. Nahar, Nutritional and Phytochemical Study of Some Edible Vegetables. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster

M. Ahasan-ul-Sakib, M. R. Islam, M. Shoeb and N. Nahar, Determination of Three Surfactant in Waste Water by Spectrophotometric Method. 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh

Poster


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M. Shoeb, N. Nahar, Emerging risks from chemical contamination of foods. International Seminar on Chemical Contaminants in Food, 20 March 2016, University of Dhaka, Dhaka, Bangladesh

Oral Yes

A. Sultana, M. Shoeb, N. Nahar, Study of dissipation patterns of diazinon in selected vegetable samples, 20 March 2016, University of Dhaka, Dhaka, Bangladesh

Oral Yes

M. Shoeb, A. Sultana, N. Uddin. S. Shaon, M. I. R. Mamun, N. Nahar, Polycyclic aromatic hydrocarbons in water of Bangladesh. (P), SETAC Europe 26 Annual Meeting, 22-26 May, 2016, Nantes, France

Poster

K. Hasan , Education and Outreach Mechanism and the approach(es) to include the Chemical Weapons Convention in the curriculum at School and University Level in Bangladesh. 14th Regional Meeting of National Authorities of States Parties in Asia, to be preceded by a regional meeting on education and outreach, Tehran, Islamic Republic of Iran, 23–25 May 2016, Iran

Oral No

M. Shoeb, N. Nahar, Chemical contaminants in the environment and in the food chain in Bangladesh. Symposium on Humanitarian Engineering, 4-5 July 2016, The University of Warwick, UK

Oral Yes

A. H. Khan, M. Shoeb, N. Nahar, Organochlorine Compounds and Heavy Metals in Sediment Samples from Coastal area of Bangladesh.SETAC Asia/Pacific 2016 Conference, 16-19 September 2016, National University of Singapore, Singapore

Poster

R. Tabassum, M. Shoeb, N. Nahar, Environmental contaminants in the aquatic ecosystems of the Bay of Bengal. SETAC Asia/Pacific 2016 Conference, 16-19 September 2016, National University of Singapore, Singapore

Poster

J. N. Jeouty, M. Shoeb, N. Nahar, Microplastic and its effect on fish samples of the coastal region surrounding Sundarban area, SETAC Asia/Pacific 2016 Conference, 16-19 September 2016, National University of Singapore, Singapore

Oral No

M. Shoeb, N. Nahar, Microplastic and its effects on the ecosys-tems of Sundarbans. SETAC Asia/Pacific 2016 Conference, 16-19 September 2016, National University of Singapore, Singapore

Oral No

S.I. Bhuyian, T. Ahmed, M. K. Hasan, M. Shoeb, N. Nahar, Spectrophotometric analysis of preservatives and coloring agents in orange soft drink and orange juice samples. 1st Symposium on Chemistry for Global Solidarity, 14 October, 2016, Department of Chemistry, Jagannath University

Poster

S.A. Mou, T. Akther, M. Shoeb, A. Sultana, N. Nahar, Studies of food preservative and coloring agent in jam and jelly samples. 1st Symposium on Chemistry for Global Solidarity, 14 October, 2016, Department of Chemistry, Jagannath University

Poster

M. A. Hossain, M. Shoeb, N. Nahar Organochlorine pesticides and fatty acid compositions in fresh water fish samples. 1st Symposium on Chemistry for Global Solidarity, 14 October, 2016, Department of Chemistry, Jagannath University

Poster

N. Nahar, Drug Discovery Programme from Antidiabetic Plants. One Day Seminar By ANRAP, BUHS, Dhaka

Oral Yes


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List conferences/workshops/courses/meetings organized by you 2015-‐June 2017

Name of event, Venue, Dates No. of participants One day Seminar on Effects of Long-‐Term Exposure of Pesticides on Farmers’ Health, Bangladesh Institute of Nuclear Agriculture, Mymensingh, Bangladesh, 04 April, 2015.

130

One day Seminar on Tandem Mass Spectrometry and its Applications for Analysis of Chemical Contaminants in Food Stuff, Department of Chemistry, University of Dhaka, Bangladesh, 02 June, 2015.

150

Farmers’ Awareness for save use pesticide, Nuritola, Comilla, Bangladesh, 09 Jan. 2016

110

ANFEC-‐NITUB Regional Training Programme on Gas Chromatography, Quantification and Quality Assurance, University of Dhaka, Dhaka, Bangladesh, 11-‐29 Jan. 2016

12

National Seminar on Chemical Contaminants in Food, University of Dhaka, Dhaka, Bangladesh, 04 Feb.2016

90

Farmers’ Awareness for toxicity of pesticide on health and Environment, Nurundi, Jamalpur, Bangladesh, 06 Feb. 2016

120

International Seminar on Chemical Contaminants in Food, University of Dhaka, Dhaka, Bangladesh, 20 Mar. 2016

60

NITUB Training Programme on High Performance Liquid Chromatography, University of Dhaka, Dhaka, Bangladesh, 27-‐31 Dec. 2016

12

Alumni Networking of Swedish Institute in Bangladesh and Promoting Environmental Sustainability, 04 May 2017, Dhaka

120


M. Shoeb, N. Nahar, Drug Discovery from Natural Products. 1st Symposium on Chemistry for Global Solidarity, 14 October, 2016, Department of Chemistry, Jagannath University

Oral Yes

N. Nahar, Chemical Safety and Security Managment.One Day Seminar Orgnazined by Bangladesh Chemical Society, 28 October 2016, BCIC Bhaban, Dhaka

Oral Yes

N. Nahar, Chemistry for Present and Our Future. Scientific Exchange Programme, Chonnam National University, 14-18 November 2016, Gwangju, Republic of Korea

Oral Yes

M. Shoeb, , Chemistry for Human Health. Scientific Exchange Programme, Chonnam National University, 14-18 November 2016, Gwangju, Republic of Korea

Oral Yes

M. Shoeb, N. Nahar, Chemical Safety and Security Management in Laboratories.Workshop on needs assessment and best practices on chemical safety and security management, 13-15 December 2016, Riga, Republic of Latvia

Oral Yes

M. Shoeb, N. Nahar, Natural Products for the Sustainable development of health care in Bangladesh. Natural Products to Combat Problems of the Developing World, 19-20 December 2016, Manchester, UK

Oral Yes

Mohammad Shoeb and Nilufar Nahar, Natural Products for Drug Discovery in Bangladesh, 38 Annual Conference of Bangladesh Chemical Society (BCS) 31 March 2017, Chittagong, Bangladesh

Oral Yes


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Have regular, scientific seminars been held at the institution? If yes, state at which academic level (BSc, MSc, PhD) and how frequent. Name of seminar series Level and Frequency Ph.D/M.Phil Research Progress Seminar at the Department Ph.D/M.Phil, Every Month Ph.D/M.Phil/ MS Students Seminar within Group Ph.D/M.Phil/ MS, Often Ph.D/M.Phil Students Seminar for Mock Viva Ph.D/M.Phil, Before Exam MS Students Seminar for Mock Viva MS, Before Exam


Describe any interaction (meetings, participation in committees, etc.) with government/society/industry/NGOs in the country, in the region or in global conventions, etc. Including unpublished reports to authorities, media exposure and public lectures etc. Were the outreach activities on your initiative or by invitation? Give account for any tangible or expected effects of outreach activities, including possibilities for policy influence. Nilufar worked as a member of Committee for policy making formed by Bangladesh Food Safety Authority and contributed to implement Bangladesh Food Safety Law 2013; invitation from the government. Nilufar gave interviews to media about safe use of pesticides, create awareness for better use and management of pesticides for human health and environment and toxicity of chemical contaminants which has been broadcasted by several popular TV channels of the country; Initiatives from the media The agreement which was made between Bangladesh Food Safety Laboratory Network (BFSLN) (financed by FAO, Dhaka) and the Department of Chemistry, University of Dhaka for analysis of pesticide residues in food stuffs in 2014 was completed in 2016 with final report. Nilufar and Shoeb attended several meeting from BFSLN. Nilufar continued her activity as member of Committee formed by Bangladesh Food Safety Authority. Shoeb was included in a Committee of Bangladesh Institute of Standard and Testing. The group members arranged meeting with farmers’ in Mymensing, Comilla and Jamalpur about safe use and toxicity of pesticides and interaction with farmers continued. Nilufar and Shoeb visited Swedish Ambassador’s Residence on 11 December 2016 after getting invitation from Mr Johan Frisell, Ambassador of Sweden to Bangladesh in honor of Alfred Nobel and Nobel Prize. Shoeb acted as Convener to organize 7th Bangladesh Chemistry Olympiad where 10,000 11 grade students participated. Shoeb Co-ordinated a meeting with all former students and researchers who studied in Sweden and formed Sweden Alumni Network in Bangladesh and acting as a Head of the Network. Mr Johan Frisell. Abnews24 published a news on September 8, 2016 that Professor Dr Mohammad Shoeb with 3 students from the group was going to Singapore to attend SETAC Conference in order to present their research finding on microplastic and its effects on the ecosystems of Sundarbans. Similar news was also published in Dhaka Tribune on September 25, 2016 including interview of Nilufar and Shoeb. There was an incident of death of fish, ducks, frogs, fowls in flash flood affected areas of Sunamgonj and Nilufar was in the fact finding Committee formed by Bangladesh Chemical Society. She visited the area with Committee members and collected samples and investigated. Later, she revealed the


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finding in a press conference on 01 June 2017 and news was published in daily newspaper the next day. An easy, quick and cheap method has been developed to identify the red dye in watermelon by Tanha and Habib, two undergrads students who were supervised by Nilufar and the news was published in the daily newspaper. Interview was taken from Nilufar and female students of the group about women education in research and published in the daily Star newspaper on 18 February 2017.


Number of visits by scientist and fellows from your group 2015-‐June 2017 To countries in the region To Sweden To other countries

<1 month

>1 month <1 month

>1 month <1 month

>1 month

No of visits

No of months

No of visits

No of month

s

No of visits

No of month

s Total 8 0 0 1 1 9 6 0 0 (Partly) on ISP money 0 0 0 0 0 0 0 0 0 Specify all visitors from your group during 2015-‐June 2017

For each visitor, provide the following: 1) Gender (female/male; F/M) 2) Title 3) Full name 4) Visited institute 5) Host of visit 6) Purpose of visit 7) Time period of visit 8) Source of funding for visit (ISP or other)

F, Dr. Nilufar Nahar, Chemistry Department, Royal University of Phnom Penh, Cambodia, to visit for ANFEC meeting to, 3 Jan-‐3 Feb 2015. The visit was funded by ANFEC. F, Dr. Nilufar Nahar, at Department of Chemistry, Mahidol University, Thailand, made a research exchange meeting with Prof Vichai Reutrakul and Associate Prof Chutima Kuhakarm 3-‐5 Feb 2015. The visit was funded by ANFEC. M, Dr. Mohammad Shoeb, Swedish Environmental Research Institute, Stockholm, Sweden, made a research visit to Dr. Mikael Remberger, 16 February 2015. No fund required as he was in Sweden. M, Dr. Mohammad Shoeb, visit to Analytical and Environmental Chemistry, Stockholm University, Sweden, to research, 02 June 2015. No fund required as he was in Sweden. M, Dr. Mohammad Shoeb, International Foundation for Sciences (IFS), Stockholm, a research visit to Dr. Cecilia Oman, Sweden, 02 June 2015. No fund required as he was in Sweden.

M, Dr. Mohammad Shoeb, visit to University of Warwick, UK, a research visit to 4-‐5 July 2016. The visit was funded by University of Warwick. F, Dr. Nilufar Nahar, visit to Chonnam National University, a research visit, 13-‐17 November 2016. The visit was funded by University Grants Commission of Bangladesh. M, Dr. Mohammad Shoeb, visit to Chonnam National University, a research visit, 13-‐17 November 2016. The visit was funded by University Grants Commission of Bangladesh.


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M, Mr. Kamrul Hasan, visit to Chonnam National University, a research visit, 13-‐17 November 2016. The visit was funded by University Grant Commission of Bangladesh. F, Dr. Nilufar Nahar, visit to College of Veterinary Medicine, Konku University, South Korea, a research visit, 18 November 2016. The visit was funded by University Grant Commission of Bangladesh. M, Dr. Mohammad Shoeb, visit to College of Veterinary Medicine, Konku University, South Korea, a research visit, 18 November 2016. The visit was funded by University Grant Commission of Bangladesh. M, Mr. Kamrul Hasan, visit to College of Veterinary Medicine, Konku University, South Korea, a research visit, 18 November 2016. The visit was funded by University Grant Commission of Bangladesh. M, Dr. Mohammad Shoeb, GRINDEX Pharmaceutical, Riga, Latvia, Industry visit, 14-‐17 December 2016. M, Dr. Mohammad Shoeb, visit to University of Manchester, UK, a research visit, 19-‐20 December 2016. M, Robiul Islam, Yunnan University, China, a visit for summer Camp to, 21-‐27 June 2016. The visit was funded by Yunnan University.

M, Mohammad Shoeb, University of Laos, Lao PDR, ANFEC Board meeting, 26 January 2017. The visit was funded by ANFEC.

F, Rafiza Islam, University of Laos, Lao PDR, ANFEC Training Programme on Gas Chromatography, Quantification and Quality Assurance, 9-‐27 January 2017. The visit was funded by ANFEC. F, Nilufar Nahar, GADC, Kyoto, Japan, visited Shimadzu Laboratory, 23-‐26 March 2017. The visit was funded by AQC, Dhaka. M, Mohammad Shoeb, GADC, Kyoto, Japan, visited Shimadzu Laboratory, 23-‐26 March 2017. The visit was funded by AQC, Dhaka.

F, Nilufar Nahar, Workstation, Singapore, visited Shimadzu (Asia Pacific) Pte Ltd., and attended training course on Principles, Operation and Maintenance of LCMS-‐8050 Triple Quadrupole UFMS with LabSolutions, 21-‐22 March 2017. The visit was funded by AQC, Dhaka. M, Mohammad Shoeb, Workstation, Singapore, visited Shimadzu (Asia Pacific) Pte Ltd., and attended training course on Principles, Operation and Maintenance of LCMS-‐8050 Triple Quadrupole UFMS with LabSolutions, 21-‐22 March 2017. The visit was funded by AQC, Dhaka. M, Mohammad Shoeb, Sweden, participated a series of meeting in Stockholm and Kiruna on the topic of the future of industry to discuss the importance of digital transformation, sustainable production and industrial skills enhancement within industry, 7-‐13 May 2017. The visit was funded by Swedish Institute.

Number of visits by scientist and fellows to your group during 2015-‐June 2017 To countries in the

region To Sweden To other countries

<1 month

>1 month <1 month

>1 month <1 month

>1 month

No of visits

No of month

s

No of visits

No of months

No of visits

No of month

s Total 3 0 0 6 8 0 0 (Partly) on ISP money

3 0 0 5 0 0 0


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Specify all visitors to your group 2015-‐June 2017 For each visitor, provide the following:

1) Gender (female/male; F/M) 2) Title 3) Full name 4) Affiliation 5) Purpose of visit 6) Time period of visit 7) Source of funding for visit (ISP or other)

M, Prof Prapin Wilairat, Department of Chemistry, Mahidol University, Bangkok, Thailand, to attend a seminar in Dept. Chemistry, Univ. Dhaka, 02 June 2015. This visit was funded by HEQEP. F, Dr. Duangjai Nacapricha, (from Center for Innovation Chemistry, Mahidol University, Bangkok, Thailand, to attend a seminar in Dept. Chemistry, Univ. Dhaka, 02 June 2015. Funded by HEQEP. M, Mr. Michael Strundell, From ACES, Stockholm University, Sweden, to discuss about ANFEC training program and checked GCMS, Chemistry, Univ. Dhaka, November 2015. The visit was funded by ANFEC. M, Prof Barry Noller, from Sustainable Minerals Institute, The University of Queensland, Australia, Chemistry, Univ. Dhaka to discuss future research collaboration, 24 November 2015. visited Dept. The visit was funded by CHEMRAWN. M, H.E. Mr Johan Frisel, Ambassador of Sweden, visited Dept. Chemistry, Univ. Dhaka, 28 January 2016. to attend Closing Ceremony of ANFEC-‐NITUB Training Programme. M, Michael Strandell, from Dept. of Environmental Science and Analytical Chemistry (ACES), Stockholm University, Sweden, visited Dept. Chemistry, Univ. Dhaka in 06-‐30 January 2016, to conduct ANFEC-‐NITUB Training Programme.The visit was funded by IPICS ANFEC. F, Ulla Eriksson from Dept. of Environmental Science and Analytical Chemistry (ACES), Stockholm University, Sweden, visited Dept. Chemistry, Univ. Dhaka, in 06-‐30 January 2016, to conduct ANFEC-‐NITUB Training Programme.The visit was funded by IPICS ANFEC. M, Dr Shanti Kongsany from National University of Laos, Laos, visited Dept. Chemistry, Univ. Dhaka, in 11-‐30 January 2016, to discuss ANFEC. The visit was funded by IPICS ANFEC. M, Dr Peter Sundin, The Director of ISP, visited Dept. Chemistry, Univ. Dhaka in 24-‐30 January 2016 to discuss ANFEC. F, Dr Cecilia Oman visited Dept. Chemistry, Univ. Dhaka in 24-‐30 January 2016 to discuss ANFEC. F, Dr Kesiny Phomkeona from National University of Laos, Laos, visited Dept. Chemistry, Univ. Dhaka to discuss ANFEC in 28-‐30 January 2016. The visit was funded by IPICS ANFEC. M, Mr. Savoeun Heng from Royal Univ. Phnom Penh, Cambodia, visited Dept. Chemistry, Univ. Dhaka, to discuss ANFEC in 28-‐30 January 2016. The visit was funded by IPICS ANFEC. M, Prof. Henrik Kylin from Linköping University, Sweden, visited Dept. Chemistry, Univ. Dhaka to discuss pesticide and other contaminants in food and environment and to attend a national seminar in 1-‐7 February 2016. The visit was funded by UGC HEQEP. M, Prof. Barry Noller from Univ. Queensland, Australia, visited Dept. Chemistry, Univ. Dhaka to discuss pesticide and other contaminants in food and environment and to attend an International Seminar in 19-‐20 March 2016. The visit was funded by UGC HEQEP. M, Dr. Ross Sadler from School of Medicine and Centre for Env. Population Health, Australia, visited Dept. Chem., Univ. Dhaka to discuss pesticide and other contaminants in food and environ-‐ment and to attend an International Seminar in 19-‐20 March 2016. Funded by UGC HEQEP. M, Prof. Jack Ng from National Res. Cen. Env Tox, Univ. Queensland, Australia, visited Dept. Chemistry, Univ. Dhaka to discuss pesticide and other contaminants in food and environment and to attend an International Seminar on 19-‐20 March 2016. The visit was funded by UGC HEQEP. M, Prof Boubakar Diawara from Paris, France, visited Dept. Chemistry, Univ. Dhaka on to discuss possible collaboration 11 April 2016.The visit was funded by Faculty of Science, DU.

Enclosure 1

Research plan/Network Program

a) Overall research questions/objective (impact) and relevance of the activity

Bangladesh is an agricultural country with a population of 160 million in 56,000 square miles. Although the economy mainly depends on the agriculture small industries for example garments, leather etc are increasing and contributing to the GDP of the country. The country is also facing natural disaster like cyclone, tornado, flood, monsoon and heavy rain, and is vulnerable for climate change. The cultivable land is decreasing day by day due to these natural disasters and urbanization. In order to meet the demand of food supply for a huge of number population, a large number of fertilizer and pesticides are being used in agricultural crops. Due to climatic condition of Bangladesh there is no other alternative than use of pesticides. Rice, pulse, lentils, grains and vegetables grow naturally, and meet the supply of local need. Fish and chicken are main protein supply of the country. But a large number of chemicals including antibiotics are also used in fish culture, chicken and beef farming. The government is ensuring food security at the present time but also giving priority to food safety with quality and nutritious value. Recently, food safety has been a burning issue in Bangladesh as toxicity of food stuff is increasing in alarming scale due to the use of toxic chemicals during food production, preservation, early fruit ripening and food storage. The situation seems to be more severe as almost every day the leading newspapers are covering a number of news about the adulteration of food, destroying huge amounts of fruits and food stuff from the market by the government officials due to the presence of unauthorized chemicals that have been used to make the foods more attractive and colorful. It is also reported that patient are increasing in hospital and doctor’s chamber due to food borne illness which can provide both acute disease like vomiting, diarrhea, dysentery and chronic disease like kidney failure, liver damage, cancer, cardiovascular disease. This may cause a burden to family and national health system and consequently effects the economy of the country. Environmental pollution is another major problem in the country. Pesticides and fertilizers usually discharge in the water body from the agricultural fields. Discharge of industrial effluents, tannery waste, fuels and surfactants in the river, lakes and cannels also contaminate water bodies. Heavy metals, toxic chemicals, and their ions (phosphate, nitrate) reduce the water quality and, thus fresh water fishes and aquatic organisms are in danger. Therefore, the research questions may arise: i) Is food safe to consume; ii) What are the level of contamination if any; iii) How to improve the food safety; iv) Do farmers use proper dose of pesticide and maintain pre-‐harvest interval; v) What is the situation of environmental pollution; vi) How to reduce the pollution; vii) How to improve laboratory facility and produce skill manpower to face the problem?

Recently, the Government has formed the Bangladesh Food Safety Authority (BFSA) in order to ensure the people’s right toward access to safe food through appropriate application of scientific processes and state of the art technology.1 Prof Nilufar Nahar, former BAN:04 Research Group Leader contributed much as a member of the Committee during preparing the Food Safety Act, 2013 followed by Safe Food Law. The group wishes to cooperate BFSA by giving scientific results.

Prior to the Food Safety Law, BAN:04 started Research work on Organic Pollutants in Food and Environment in Bangladesh under the leadership of Prof Nilufar Nahar in 2003 after getting financial supports from ISP and other sources. Initially, the group developed methods to analyze organohalogen compounds including pesticides and PCBs in fish, soil and waters samples, modern pesticide residues and their dissipation pattern in food stuffs, ripening agent, nitrofuran antibiotics, etc.2-‐6 The group members also successfully completed two project works in collaboration with the

Department of Environment (DoE), Government of Bangladesh (GOB) and with the Ministry of Fisheries and Livestock (MOFL), GOB. Later, the group extended research work and developed laboratory infrastructure with GC with ECD and FID detectors, LC with PDA and Fluorescence detector, and GC with mass detector (GC-‐MS) and LC-‐MS/MS. Staffs and students of the group got training at home and abroad and developed skills in residue analysis. The Group is also playing an important role for ANFEC. Due to research capability in terms of scientific knowledge and instrumental facility the Government of Bangladesh needs contribution from BAN:04 Research Group for ensuring Food Safety and reduce of environmental pollution. So the overall objective is to improve the status of food safety and quality, and reduce environmental pollution in Bangladesh. To achieve this, the strategy is to develop the instrumental facilities and to produce skilled manpower in order to work on chemical contaminants/organic pollutants in food and environment. The Government is also promoting students and staffs by introducing fellowship program for PhD students and supporting young researcher by small research grants to produce skill manpower in the analytical field and enhance scientific knowledge. The activity of the project will foster the Government policy to develop scientific knowledge. Thus, the overall objectives of the BAN:04 is very much relevance to the need of the country. b) Specific research questions and objectives

i) to develop/establish analytical methods for identification and quantification of different toxic contaminants like pesticides, organohalogen compounds, antibiotics, aflatoxins, polycyclic aromatic hydrocarbons, microplastic, phthalates etc. in food and environmental samples.

ii) to foster post graduate education plan by giving training to Ph.D. M. Phil. and MS students and also undergraduate students, and produce skilled manpower. iii) to create awareness among the farmers and food producers about the short and long term harmful effects of chemical contaminants on human health and environment. iv) to actively participate in the ISP supported network ANFEC in order to increase the research capacity and staff training. v) to maintain and increase South-‐South and North-‐South collaborations by staff and student exchange program. vi) to disseminate the research finding by publishing scientific papers in peer review journals and organizing/participating seminar/conferences. vii) to contribute to the Government/society during policy making by giving scientific data and suggestion to improve food safety and reduce environmental pollution.

c) Planned outputs and expected outcomes

Post graduate program will be continued to attract MS and PhD students who will learn techniques and develop methods to analyze samples. It is expected that Ph.D. (4), M.Phil. (2), MS (20) and BS (4th Year project; 20) students (n=46) will carry out research and produce theses and will be graduated. Existing methods will be modified, new methods will be devolved and validated. Samples (fish, soil, water, vegetables, chicken, beef, and processed food etc.) will be analyzed for the presence of residual contaminants and results will be included in the students’ theses. Scientific data will be generated which will be provided to BFSA/concern authority to improve the status of food safety and reduce environmental pollution. Research findings will be published in peer reviewed

journals and presented in local, regional and international seminar, symposium or workshops. South-‐South and North-‐South exchange program for students and staff members and advance training of post-‐graduate students will be arranged. ANFEC Fellow and Minor Field Study (MFS) students from Sweden or other country will be welcome to take research training. Scientists from the North and/or from South will be invited to deliver lectures on current issues of food contaminants and environmental pollutions. Scientific results will be shared with BFSA/governmental officials/policy makers/media by means of meeting/seminar/conference. Meeting and workshop with farmers will be arranged in rural level to create awareness about safe use of chemical contaminants and their toxic effects on human health and environment. Female students will be encouraged to do MS/PhD and participate in training and seminar to improve their skills.

d) Background

Farmers in Bangladesh usually use an excessive amount of pesticide in agricultural products. Overuse of pesticides is a risk factor to farmers’ health as well as for the environment.7 BAN:04 project started work with the analysis of residual organochlorine compounds (OCs) in fish (fresh & dry) and vegetable samples. Bioaccumulation of OCs in fatty tissues of fish and fowls and human blood were also analyzed. Later, organophosphorus, pyrethroid and carbamate groups of pesticide and their dissipation patterns were analyzed in several matrices like rice, vegetable and tea samples in collaboration with Governmental organizations. Heavy metals were also analyzed in rice, water, soil and fish samples. Getting supports from ISP and other sources, the group enhanced the instrumental facilities and upgraded the laboratories.

The group consists of 6 faculty members with 4 PhD and one skilled technical Officer. All members are competent enough to carry out research activity in the proposed field. Eight PhD and three MPhil students completed their program and got degrees. About 10-‐12 MS and 6-‐10 BS (4th Year) students carry out their research work on food and environmental contaminants each year and submit their theses (and projects) for degrees. They developed their skill and acquired knowledge to utilize at job and thus contribute for sustainable development of the country.

The group has expanded its collaboration in the regional (Cambodia, Lao PDR and Republic of Korea) and international (Sweden, Tanzania) levels. Many foreign students and faculty members visited the group and got training on pesticide residue analysis in various matrices. Undergraduate students from USA and Sweden have carried out their Minor Field Study at the laboratory of BAN:04 group. The group is contributing to ANFEC from its inception and a joint ANFEC NITUB training program on GC, Quality Control and assurance was held in 2016. I am contributing to the group as potential member, later as Deputy Leader and now as a Group Leader. I am experienced in analytical and environmental chemistry, got advanced training at home and abroad, supervised many PhD, M.Phil, MS and BS students. I have dynamic leadership capability for motivating and managing research Team, organizing and managing events, planning for research and applying for research grants. I have strong collaboration with local and International scientific community. Our institute has excellent infrastructure. The programme is suitable under my leadership at our institute. However, we need some more equipments like GC-‐ECD, HPLC-‐PDA, TOC and FT-‐IR, and postgraduate training to carry out activity to meet the future challenges. e) Strategy and plan

The research work will be carried by Post Graduate (PhD, M.Phil and MS), undergraduate (4th Year BS) students and research fellow under our supervision. They will develop and validate new

methods and analyse samples. Other than research work students will also attend seminar/workshop to present paper and get advanced training on relevant fields at home and abroad. It is planned that during 2018-‐2020, four Ph.D.s (Two male and two female), two M. Phil. (one female and male), 20 MS and 20 4th year project students will perform research on different types of contaminants in food and environmental. However, number may increase if new students show their interest and join the group. New equipments/instruments will be purchased and north-‐south and south-‐south collaborations will be strengthened and increased to foster the research activity.

Samples will be collected from different region of Bangladesh following standard protocol and all samples will be stored in -‐20 0C temperature in a freezer. Standards of targeted pesticides/antibiotics/other contaminants will be purchased. Samples will be extracted and cleaned up following the methodologies available in the literature and our earlier studies, and will be developed if necessary. The extracted and cleaned up samples will be analyzed by gas chromatography (GC), gas chromatography mass spectrophotometer (GCMS), high performance liquid chromatography (HPLC) and liquid chromatography mass spectrophotometer (LC-‐MS/MS). Relatively polar compounds will be analyzed by HPLC. LC-‐MS/MS and GC-‐MS will generally be used for confirmation of standards and pesticides/antibiotics. Multiple reaction monitoring (MRM) method will be used for LCMS/MS analysis and SIM mode will be used for GCMS. Limit of detection (LOD), limit of quantification (LOQ) and percentage of recovery will be determined, and quality control and quality assurance will be maintained during each study. Some of planed work and procedures to be used are as follows:

1. Analysis of antibiotics in poultry, beef and fish samples

Antibiotics are frequently used in food producing animals. Residual drugs above the allowable limit reduce Food Safety & Quality. Sulfa drugs (sulfadiazine, sulfadimethoxine, sulfamethazine, sulfamerazine, sulfamethiazole and sulfamethoxypyridiazine), chloramphenicol, tetracyclin, oxytetracyclin, nitrofuran antibiotics will be analyzed in chicken meats, beef, eggs and fish samples by HPLC and LCMS/MS following the methodologies found in the literature or developed methods.8-‐14 Multiple Reaction Monitoring method (MRM) will be used to analyze samples by LCMS/MS.

2. Analysis of aflatoxins in food and food products Aflatoxins are a subclass of mycotoxins mainly produced by the fungi Aspergillus flavus and A. parasiticus. Aflatoxins occur naturally in most of the commodities including grain, soybean and nuts. The climatic condition of Bangladesh favors the growth and possible contamination of foodstuffs by aflatoxins. Due to their high toxicity and carcinogenicity, aflatoxins are of major concern for food producers, food processing industries, and consumers. Aflatoxins (B1, B2, G1 and G2) will be analyzed in stored rice grain and nuts. Samples will be extracted with aqueous methanol and purified by immunoaffinity column by following the officially recognized ISO 16050 method.15 Identification and quantification of aflatoxins; B1, B2, G1 and G2 samples will be done by reverse-‐phase high performance liquid chromatography where KOBRA Cell is attached after column for post-‐column bromo derivatisation (PCD) which gave fluorescence.16-‐19

3. Studies of pesticide residue and their dissipation patterns in local vegetables

Pesticides (diazinon, chlorpyrifos, cypermethrin, fenvalerate, emamectin /evermactin) are used in vegetable (bean, cauliflower, tomato, egg plants etc) cultivation. During the last granting period dissipation pattern of diazinon, quinalphos, chlorpyrifos, cypermethrin were studied in several vegetables under a collaborative program with Bangladesh Agricultural Research Institute. Collaborations will be continued and dissipation pattern of several other pesticides will be carried out. Vegetable will be grown in the experimental fields (treated and control) and pesticides will be

sprayed. Both control and treated samples will be harvested from the experimental fields according to WHO guidelines. The vegetable samples will be analyzed by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) extraction method and cleaned-‐up by dispersive solid phase extraction method using PSA and by column chromatography using alumina, florisil & charcoal.20-‐24 The pesticides will be analyzed by GC-‐ECD, LC-‐PDA and LC-‐fluorescence detector. Vegetable samples will also be collected from farmers’ field and markets, and will be analyzed.

4. Analysis of emerging contaminants in marine environment of the Bay of Bengal

The Bay of Bengal is located at the northeastern end of the Indian Ocean and has an area of 2,172,000 km². The coastal areas of Bangladesh include 60% of the Sundarbans, the mangrove forest of the Ganges/Brahmaputra delta in the west. These areas are economically important because of fishing and biodiversity due to rich source of flora and fauna. Recently, marine plastic debris and microplastics have risen as one of the most important environmental issues globally. Several studies have covered the situation in the Pacific and Atlantic Oceans, but very little is known about the situation in Bay of Bengal. We will study emerging contamination including microplastics, phthalates, organochlorine compounds, PAHs, heavy metals etc. on the sensitive ecosystems of the Sundarbans. For microplastic in fish samples, gastrointestinal tracts of fish species will be digested in 10% KOH at 60 0C for 24 hours followed by filtration on Millipore membranes (0.45 μm) and finally dried materials were analyzed by FT-‐IR.25 Organochlorine compounds (DDTs and PCBs) and heavy metals (Cd, Cr, Hg As and Pb) will be analyzed by GC-‐ECD and AAS, respectively. Methods will be developed or followed from the literature for analysis of phthalates in samples.26-‐29 5. Analysis of other contaminants in foodstuff Additives such as coloring agents, preservatives, artificial sweeteners, and simulants are often intentionally used in processed food and other food stuff. Most of these chemicals have toxic effects on human health especially children. Analysis of benzoic acid, sodium benzoate, saccharin, aspartame, acesulfame-‐K and caffeine carbonated beverages, fruit juices will be carried out by UV-‐Vis spectrophotometer and HPLC following AOAC and other standard procedures standard procedures.30-‐33 6. Study of water quality and chemical contaminants in aquatic environment of Bangladesh In recent years, aquatic environment pollution with various types of chemical contaminants such as marine debris, micro plastics, heavy metals, persistent organic pollutants (POPs, such as DDT, dioxins, etc.), phthalates, and polycyclic aromatic hydrocarbons has become a worldwide problem and can deteriorate water quality and harmful for biota. Considering this issue, common water quality parameters such as pH, conductance, total organic carbon, ions (phosphate, nitrate, sulphate) will be examined in addition to chemical contaminants to discuss the quality of water.34-‐37 Phthalates, PCBs, DDTs will be analyzed by GC-‐ECD and PAH by GC-‐FID. The presence and concentration of heavy metals such as Pb, Cr, Cd, As, Hg will also be analyzed by AAS.38 f) Gender balance Application is submitted for extra funds. Please see Enclosure 3. g) Expected funding

The salary of the staff members and other logistic supports are available from the university and thus, in-‐kind contribution is approximately 68,000 USD per year. The university is paying Tax and Vat for customs clearance at the airport when equipment is purchased from abroad. Small glass ware and few consumable

items can also be obtained from the university. Thus, contribution from the university may be 15,000 USD per year. It is expected that some funding will come from the Ministry of Science and Technology, Govt. of Bangladesh (15,000 USD), University Grants Commission (UGC; 12,500 USD) and Bose Centre for Advance Studies (5000 USD) during the project work. During the last granting period (2015-‐2017) a research of 400,000 USD was received from HEQEP, UGC project. We can apply for extra fund for sustainability of our project. We have plan to apply to OPCW, the Netherlands, Royal Society of Chemistry, UK and European Union for Research Grants. Ph.D. and MS students apply to Ministry of Science and Technology for Fellowship.

h) Collaboration with other scientists

The group has strong collaborations with the scientists from Cambodia, Laos and South Korea. Scientists from Cambodia, Laos and South Korea visited Bangladesh and staffs from group also visited there. Two Ph.D. and one M.Phil students visited Prof Shim Laboratory in Korea earlier. To strengthen the regional collaboration, Asian Network of Research on Food and Environmental Contaminants is working smoothly. Shoeb visited Linkoping University for Post Doc under Professor Henrik Kylin and also University of Warwick and collaborations were extended to Linkoping University and Warwick University. New collaboration will make with Sorya (Cambodia) as she is New Group Leader, with Barry and Stewart (Australia) as they are also running Food Network named APFAN and common issues and staff/students exchange will be increased, with Christine (Ireland) as she is working in the same field and likes to collaborate with student exchange, with Farhan (Denmark) to work for microplastic, with Cecilia (Sweden) for to work on ecotoxicology, with Marcel (UK) as he has excellent instrumental facility and with Matin (Bangladesh) to work on heavy metals.

i) Postgraduate students

Please see the Enclosure 1 (i)

j) Justification of budget items

Please find in the form and elaborate justification in Enclosure 1 (j).

References

1. Bangladesh Food Safety Authority (2015), http://www.bfsa.gov.bd/ 2. Shoeb, M., Md. Amjad Hossain, Md. Golam Kibria, Tonima Mustafa and Nilufar Nahar, (2017)

, Bioaccumulation of Mercury in Fish Species from Different Tropic Level, Journal of Food Science and Engineering 17-‐28.

3. Shoeb M, Al Mamun M S, Noor R E, Al Mahmud M I R, and Nahar N, (2017), Residual DDTs and fatty acid compositions in popular two fish samples, Dhaka Univ. J. of Sci., 65 (1) 77-‐80.

4. Hossain M. A, Shoeb M. and Nahar N., (2016), DDT and Its Metabolites in Fresh Water Fish Samples J. Food Sci. and Engineering 6, 344-‐350.

5. Nahar N, Hossain M M, Uddin-‐Al-‐Mahmud, M N, Shoeb M, Latifa G A and Kabir, K H, (2016) Dissipation of Cypermethrin in Bean and Cauliflower, Dhaka Univ. J. Sci, 64(1):89-‐90

6. Shoeb, M., Nahar, N., Mandal, S, Sarma, H., M. and Seraji, M., S., I., (2009), Nitrofuran antibiotics and organochlorine pesticides in Fish and Poultry Fish, Feed ingredients, and water samples collected from shrimp cultivation area, Journal of Bangladesh Chemical Society, , 22 (1), 1-‐8.

7. Nahar, N. (2006). Survey and research on DDT and PCBs in food items and environmental

samples, Department of Environment, Ministry of Environment and Forest, Government of Bangladesh.

8. Vora V.R. and Raikwar, M.K. (2013) "Determination of Chloramphenicol and Thiamphenicol

Residues in Fish, Shrimp and Milk by ESI-‐LCMSMS" International Journal of Agriculture and Food Science Technology 4.8: 823-‐ 828

9. Neuhaus B.K., Hurlbut. J.A. and Hammack W., September (2002), LC/MS/MS Analysis of Chloramphenicol in Shrimp, U.S. FDA Laboratory Information Bulletin No. 4290, Volume 18, No. 9,

10. Evangelos Gikas et al, (2004), Development of a Rapid and Sensitive SPE-‐LC-‐ESI MS/MS Method for the Determination of Chloramphenicol in Seafood, J. Agric. Food Chem., 52 (5): 1025–1030

11. Cavaliere, C., Curini, R., Corcia, A. D., Nazzari, M., & Samperi, R. (2003). A simple and sensitive liquid chromatography-‐mass spectrometry confirmatory method for analyzing sulfonamide antibacterials in milk and egg. Journal of Agricultural and Food Chemistry, 51, 538-‐566.

12. Forti A.F. and Scortichini, G., (2009). Determination of ten sulphonamides in egg by liquid chromatography-‐tandem mass spectrometry. Analytica Chimica Acta, 637, 214–219.

13. Thompson, T.S., and Noot, D.K., (2005). Determination of sulfonamides in honey by liquid

chromatography–tandem mass spectrometry. Analytica Chimica Acta, 551, 168–176.

14. Van Rhijn, J.A., Lasaroms, J.J.P., Berendsen, B.J.A., and. Brinkman, U.A.Th., (2001).Liquid chromatographic–tandem mass spectrometric determination of selected sulphonamides in milk. Journal of Chromatography A, 960, 121-‐133.

15. ISO 16050, 2003 (E). Foodstuffs -‐ determination of aflatoxin B1, and the total content of aflatoxins B1, B2, G1 and G2 in cereals, nuts and derived products -‐ High-‐performance liquid chromatographic method. First edition.

16. Aycicek H., Aksoy, A. & Saygi, S. (2005). Determination of aflatoxin levels in some dairy and food products which consumed in Ankara, Turkey, Food Control, 16:263-‐266.

17. Herrman T. (2002), Mycotosins in feed grains and ingredients. Department of Grain Science

and Industry, Kansas State University Agricultural Experiment Station and Cooperative Extention, MF-‐206.

18. Tam J., Mankotia M., Mably M., Pantazopoulos P., Neil R. J., Calway P., and Scott P. M.,

(2006), Survey of breakfast and infant cereals for Aflatoxins B1, B2, G1 and G2, Food Additives and contaminants, 23(7): 693-‐699.

19. Bonnet E. S., Carvajal M., Ramirez I. M., Urueta P. C., Eslava J. C., Arroya S. G. and Vara J. M. M. (2013), Aflatoxin (B1, B2, G1 and G2) Contamination in Rice Contamination in Rice of Mexico and Spain from Local Sources or Imported, Journal of Food Science, 78: 11

20. Anastassiades M, Lehotay S J, Stajnbaher D, Schenck F J (2003) Fast and easy multiresidue method employing acetonitrile extraction/ partitioning and “dispersive solid-‐phase extraction” for the determination of pesticide residues in produce. J. AOAC Int. 86: 412–431

21. Akerblom, M. (1995). Environmental monitoring of pesticide residues—guidelines for the SADC region. Uppsala: Department of Environmental Assessment, Swedish University of Agricultural Sciences

22. Xie X., Wang X., and Zhao L., (2011), Food Analytical Methods, 4(2):203-‐211. 23. Erzen N. K. and Flajs V. C., (2003), Simultaneous determination of abamectin and doramectin

in soil from a grazed pasture, Acta Chim. Slov. 50:301-‐308.

24. Mahmud M. N. U. A., Rahman M., Tae-‐Woong, N., Park, J. H. , Yang A., Park, K. H., Abd El-‐Aty A. M., Nahar N. and Shim, J. H., (2013), A QuEChERS-‐based extraction method for the residual analysis of pyraclofos and tebufenpyrad in perilla leaves using gas chromatography: application to dissipation pattern. Biomed. Chromatogr. 27: 156-‐163.

25. Fares John Biginagwa, Bahati Sosthenes Mayoma, Yvonne Shashoua, Kristian Syberg, Farhan R. Khan, (2016) First evidence of microplastics in the African Great Lakes : Recovery from Lake Victoria Nile perch and Nile tilapia", Journal of Great Lakes Research 42146-‐149.

26. La Fleur AD, Schug KA, (2011). A review of separation methods for the determination of estrogens and plastics-‐derived estrogen mimics from aqueous systems. Anal Chim Acta, 696: 6–26.

27. Guo Y, Zhang ZF, Liu LY, Li YF, Ren NQ, Kannan K, (2012). Occurrence and profiles of phthalates in foodstuffs from China and their implications for human. J Agric Food Chem, 60: 6913–9.

28. Wu X, Hong H, Liu X, Guan W, Meng L, Ye Y, (2013). Graphene-‐dispersive solid-‐phase extraction of phthalate acid esters from environmental water. Sci Total Environ, 444: 224–30.

29. Kambia K, Dine T, Gressier B, Germe AF, Luyckx M, Brunet C, et al., (2001). High-‐performance liquid chromatographic method for the determination of di(2-‐ethylhexyl) phthalate in total parenteral nutrition and in plasma. J Chromatogr,755:297–303.

30. Knezevic, Z., Serder, M.(2011), Determination of artificial sweeteners in beverages and special nutritional product using high performance liquid chromatography. Arh. Hig Rada Toksikol, 62:169-‐173

31. Fruit and vegetable products-‐ Determination of benzoic acid and sorbic acid concentrations-‐ High Performance liquid chromatography method, ISO 22855:2008(E)

32. AOAC official Method 979.08, (2005). 33. Knezevic, Z., Serder, M. (2011), Determination of artificial sweeteners in beverages and

special nutritional product using high performance liquid chromatography. Arh. Hig Rada Toksikol, 62:169-‐173

34. Diana Florescu, Andreea Maria Iordache, Diana Costinel1, Elena Horj, Roxana Elena Ionete, Monica Culea, (2013) Validation Procedure For Assessing The Total Organic Carbon In Water Samples,Rom. Journ. Phys., Vol. 58, Nos. 1–2, P. 211–219, Bucharest,

35. ISO 8245:2000, Water quality – Guidelines for the determination of total organic carbon (TOC) and dissolved organic carbon (DOC), 2000.

36. D. Chapman and V. Kimstach, (1999), Water Quality Assessments -‐ A Guide to Use of Biota, Sediments and Water in Environmental Monitoring, 2nd Edition, UNESCO/WHO/UNEP

37. APHA. Standard methods for the examination of water and wastewater. 19th ed. Published by the American Public Health Association. Washington: DC; (1995).

38. American Public Health Association (APHA) (2005) Standard method for examination of water and wastewater, 21st edn. APHA, AWWA, WPCF, Washington

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Enclosure 1 (h)

Postgraduate Students

Ph.D. Students

1. Md. Shahed Reza (Male; E-mail: [email protected])

Title: Studies on Contaminants (Multi Pesticide Residue) in Fruits and Vegetable

Year of Registration: 2017

Expected Year of submission: 2021

Summary of work

Md. Shahed Reza joined the group in 2016 and registrared for PhD program in 2017. Objective of his research work is to determine the dissipation pattern and residue level of some commonly used pesticides in fruit and vegetable samples which will be collected from different region of Bangladesh for evaluation of residue levels and from experimental field of Bangladesh Agriculture Research Institute (BARI) for determination of dissipation patterns. He will also work to modify Quick, Easy, Chief, Effective, Rugged and Safe method (QuEChERS) method for analysis for multi residues to make the analysis easy and effective. He learnt how to use sophisticated analytical instruments like HPLC, GC, GC-MS and LC-MS/MS and will use for his own research as well. He already validated a multi residue QuEChERS method utilizing GC-MS (EI) consisting sixteen(16) organophosphorus (OPPs) pesticides which includes Methamidophos, Acephate, Ethoprophos, Dimethoate, Diazinon, Methyl Parathion, Metalaxyl, Fenitrothion, Malathion, Fenthion, Chlorpyrifos, Quinalphos, Methidathion, Fenamiphos , Ethion and Propiconazole. At the moment he does not need any train abroad and support during PhD program.

2. Rafiza Islam (Female; E-mail: [email protected])

Title: Investigation of Commonly Used Antibiotics in Meat, Egg and Milk Samples



Summary of work

Rafiza Islam joined the group in 2016 and registrared for PhD program in 2017. Objective of her research is to investigate of Antibiotic Residues in Meat, Milk, and Egg Samples which will be collected from poultry farm and slaughter house in different local markets of different

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area in Bangladesh. Extraction of the antibiotics in three different matrices (egg, milk and meat) will be carried out by solid phase extraction method and QuEChERS method. Moreover, she will work on method development and validation of analyzing of antibiotics if required. Identification and quantification of antibiotics will be done with the help of HPLC and LC-MS/MS. She attended ANFEC Training programme on Chromatography for her own research. She needs training in abroad and fellowship to support herself. So ISP support is required for her during PhD program.

3. Md. Mizanur Rahman (Male; E-mail: [email protected])

Title: Isolation and Structure Elucidation of Secondary Metabolites from Three Medicinal Plants



Summary of work

Md. Mizanur Rhaman joined the group in 2016 and registered for PhD program in 2017. He is investigating Morus alba, Averrhoa bilimbi and Peperomia pellucida plants which are abundantly distributed in Bangladesh. But still now the phytochemical information of these plants is not properly investigated. Therefore isolation and characterization of the different compounds from these plants are very important. Isolation of the secondary metabolites from these plants will be carried out by chromatographic technique and compounds will be characterized by modern spectroscopic technique i.e. UV, IR, NMR (1D & 2D) and mass spectroscopic studies. Antidiabetic, antioxidant activity, antimicrobial activities, cytotoxicity assay to evaluate biological properties of these plants will also be carried out. He has participated the 62nd Training Programme of NITUB, on the use maintenance and trouble-shooting of common Medical Instruments held on 26 November-01 December 2016, BUHS, Dhaka, Bangladesh. It is too early to write about his progress. He got fellowship from University Grants Commission of Bangladesh. At the moment he is getting indirect support but does not need any direct support from ISP other than participation at training program during PhD program.

M. Phil Student

4. Farhana Sobnom (Female; E-mail: [email protected] )

Title: Level of organophosphorus insecticides in the diet and blood samples of an adult rural population in relation to their cardiovascular risk


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Summary of work

Cardiovascular diseases (CVDs) are now important medical and public health issues and those constitute one of the leading causes of death throughout the world. In developing countries like Bangladesh both the incidence and prevalence of CVDs are increasing rapidly. Recent data indicate CVD prevalence between 1.85% and 3.4% among rural and 19.6% among urban working professionals in Bangladesh. A link between pesticide exposure and cardiovascular risk has been postulated for a long time. Over 100 organophosphorus compounds are being used for both agricultural and landscape pest control. The general objective of the study is to explore the association of cardiovascular risk with organophosphorus insecticide exposure among an adult rural Bangladeshi population. Farhana just started her M.Phil. program and will have to attend theoretical courses. Once she has passed exam she will be eligible to submit dissertation. She is doing literature review now. At the moment she is getting indirect support but does not need any direct support from ISP other than participation at training program during MPhil program.

M.S. Students

5. Mofizul Islam (Male; E-mail: [email protected])

Title: Analysis of Artificial Preservatives, Sweetening and Stimulating agents in Carbonated Beverages by HPLC



Summary of work

Carbonated beverages are popular drink all over the world including Bangladeshi. These beverages contain many types of food additives, for example: artificial preservatives, sweetening and stimulating agents. Use of many preservatives, sweetening and stimulating agents above to a certain level is harmful for health. The consumption of artificial sweetened beverages has been linked to the risks of obesity, diabetes and heart diseases etc. So it is very essential to know the level of these preservatives, sweetening and stimulating agents in carbonated beverages available in the market to avoid the adverse health effect. In the present study in different brands of drinks (Pepsi cola, RC cola, RC Lemon, Coca-Cola, 7up, Mountain Dew, Speed, Tiger, Black horse, Mirinda, Funta, Urocola, and Upper10) will be screened for the presence Benzoic acid, Sodium benzoate, Saccharin, Aspartame, Acesulfame-K and Caffeine by HPLC following standard procedures. He has developed methods and is analyzing samples from markets. He has to sit for theoretical examination in September but will be able to submit dissertation on time. At the moment he is getting

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indirect support but does not need any direct support from ISP other than participation at training program.

6. Salma Akter Mou (Female; E-mail: [email protected])

Title: Analysis of Sulfadrugs and Chloramphenicol in Poultry Meat and Beef Samples.



Summary of work

Sulfa drugs and chloramphenicol are fed to broiler chicken and livestock animals mixed with food for rapid growth. Residual drugs above MRL are health hazardous and reduce food safety. Six sulfa drugs namely, sulfadiazine, sulfadimethoxime, sulfamethazine, sulfamerazine, sulfamethiazole, sulfamethoxypyradiazine and chloramphenicol will be analyzed by Liquid Chromatography coupled with tandem Mass Spectrometer (LC-MS/MS). Shimadzu protocol will be modified and validated in terms of MS parameters and mobile phase composition. Extraction will be done by QuEChERS method and cleaned up with C-18 cartridge & PSA. Separations were carried out in C18 and column oven temperature and flow rate of mobile phase were set at 40 0C and 1 mL/min, respectively. Mobile phase is an isocratic elution of methanol -water (40:60 ratio). The analysis mode is positive and ionization process was electronspray ionization. Nebulizing, Drying and Heating gas flow were set at 3, 10 and 10 L/min, respectively. Interface, DL and Heat block temperature were set at 300, 250 and 4000C. Validation of the method will be carrying out for linearity, accuracy, precision (repeatability and reproducibility) and sensitivity. LOQ and LOD will also be estimated. A major portion of her work has been finished. She has to sit for theoretical examination in September and will submit dissertation after examination. She is getting fellowship from ISP supported project BAN:04 and needs to continue.

7. Murshid Hossain (Male; E-mail: [email protected])

Title: Method development and validation for the analysis of artificial sweeteners and preservatives in fruit juice using HPLC



Summary of work

Fruit juice is a beverage made from the extraction or pressing out of the natural liquid contained in fruit. Artificial color and synthetic sweetener are added in the commercial

5

juice in addition to natural fruit juice to make the juice more attractive, tasty and this helps the commercial producer to make more profit. The aim of this study is the quantitative determination of concentration of various additives presence in commercial fruit juice available in Dhaka city by HPLC. He has developed method following literature and analysing samples. He has to sit for theoretical examination in September but will be able to submit dissertation on time. He does not need any support from ISP now. 8. Saiful Islam Bhuyian (Male; E-mail: [email protected])

Title: Method development and validation for determination of Chloramphenicol in Shrimp and Prawn samples by HPLC



Summary of work

Freshwater prawn (Macrobrachium rosenbergii) and marine shrimp (Penaeus mondon) together represent the second largest exportable item contributing about 5% to Gross Domestic Production (GDP) in 2016 in Bangladesh. Targeting mainly for export, prawn and shrimp aquaculture has been expanded very fast over last decades.The antibiotics used in aquaculture to prevent or control diseases can directly impact the environment when they are improperly used. Chloramphenicol (CAP) is commonly used in aquaculture as a prophylactic or disinfectant to prevent diseases, or as a chemotherapeutic agent to control diseases. Although CAP is extensively metabolised by aquatic animals, residues left in the body and direct contamination of the environment may still be a concern. For this reason, the objectives of the proposed research project are: 1.To determine the presence of CAP in shrimp and prawn in the southwest coastal region of Bangladesh 2. To determine the quantity of CAP in shrimp and prawn 3.Statistical analysis to evaluate the health risk based on consumption and cancer limit risk 4.Validation of an established method of CAP by using HPLC. A simple, accurate, and precise stability-indicating HPLC analytical method has been developed and is trying to validate. He has to sit for theoretical examination in September but will be able to submit dissertation on time. He does not need any support from ISP now.

9. Tofael Ahmed (Male; E-mail: [email protected])

Title: Bioactive compounds from Morus alba (Tut) Year of Registration: 2017 Expected Year of submission: 2018

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Summary of work

Morus alba belongs to the family of Moraceae. It is medium sized tree, 3-6 m high with whitish or dark purple fleshy fruits. M. alba is available in Bangladesh. The plant is used in folk medicine for the treatment of diarrhea, asthma, cough, dyspespsia, eye problems, and intestinal ulcers. So this plant has great importance in the field of medicine. Therefore, the objectives of the present research work are to: Separate, purify and isolation of active compounds from extracts of M. alba, identify and elucidate structures of isolated compounds by different spectroscopic techniques including one dimensional and two dimensional NMR and performing antidiabetic, antioxidant and antibacterial activities of the extracts and pure compounds. Dried materials were extracted with aqueous 80% ethanol and then partitioned in between water and dichloromethane (DCM), and both extracts were separated. Water part was partitioned again with n-butanol and butanol, DCM part were dried and were partitioned in between aqueous 90% methanol and hexane. The aqueous 90% methanol was adjusted to methanol: water (50:50) and partitioned with DCM. Finally, methanol and DCM extract were obtained. Different chromatographic techniques, e.g. thin layer chromatography, vacuum liquid chromatography, column chromatography and high performance liquid chromatography (HPLC) will be applied for separation, purification and isolation of pure and active compounds from these extracts. Modern spectroscopic techniques i.e. UV, IR one and two dimensional NMR and mass spectrometry will be used for structure elucidation of compounds. He has to sit for theoretical examination in September but will be able to submit dissertation on time. He does not need any support from ISP now.

10. Farhana Sharmin (Female; E-mail: [email protected])

Title: Chemical contaminants in the aquatic environment of the Southern part of Bangladesh Year of Registration: 2017


Summary of work

In recent years, aquatic environment pollution with various types of chemical contaminants such as marine debris, micro plastics, heavy metals, persistent organic pollutants (POPs, such as DDT, dioxins, etc.), phthalates, and polycyclic aromatic hydrocarbons has become a worldwide problem. Considering this issue, common water quality parameters such as pH, conductance, total organic carbon will be examine to discuss the quality of water and measure the pollution. To determine the polychlorinated biphenyl (PCB) or persistent organic pollutants such as DDT in water matrices, samples will be collected from the southern part of Bangladesh and studied using GC-ECD. Concentration of polycyclic aromatic hydrocarbons in water samples will be determined using GC-FID. The presence and concentration of heavy metals such as Pb, Cr, Cd, As, Hg & the concentration of phthalates in the environment will be studied using atomic absorption spectroscopy (AAS) instrument. Constant monitoring of water quality of the southern part with the mangrove forest & the Bengal coast is

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immediately needed to record with a view to minimize the risk of health of the population and the detrimental impacts on the aquatic environment. She has to sit for theoretical examination in September but will be able to submit dissertation on time. She does not need any support from ISP now. But after getting MS degree she likes to join the group for PhD programme in 2018 and ISP support for fellowship and training will be required. B.S. Students (4th years project) 11. Rakibul Islam (Male; E-mail:[email protected])

Title: Study of chemical pollutants in drinking water



Summary of work

Rakibul Islam is a 4th year project student and recently joined the group. He is now attending his theoretical class as well as project work on the above title. It is too early to write about his progress. He does not need any financial support. At the moment he is getting indirect support but does not need any direct support from ISP.

12. Md. Hossan Sohid Shoraoardy (Male; E-mail: [email protected])

Title: Study of water quality parameter



Summary of work

Md. Hossan Sohid is a 4th year project student of the group. He is now attending his theoretical class as well as project work. At the moment he is getting indirect support but does not need any direct support from ISP.

13. Md. Rezaul Alam Rifat (Male; E-mail: [email protected])

Title: Analysis of additives in processed food



Summary of work

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Md. Rezaul Alam Rifat is a 4th year project student of the group. He is now attending his theoretical class as well as project work. At the moment he is getting indirect support but does not need any direct support from ISP.

14. Md. Farhanul Alam (Male; E-mail: [email protected])

Title: Analysis of additives in processed food



Summary of work

MD. Farhanul Alam is a 4th year project student of the group. He is now attending his theoretical class as well as project work. At the moment he is getting indirect support but does not need any direct support from ISP.

15. Palash Chandra Nandi (Male; E-mail: [email protected])

Title: Bioactive compounds from natural products



Summary of work

Palash Chandra Nandi is a 4th year project student of the group. He is now attending his theoretical class as well as project work. At the moment he is getting indirect support but does not need any direct support from ISP.

16. Khandokar Tahmina Tasnim (Female; E-mail: [email protected])

Title: Chemical contaminants in food



Summary of work

Khandokar Tahmina Tasnim is a 4th year project student of the group. She is now attending her theoretical class as well as project work. At the moment she is getting indirect support but does not need any direct support from ISP.

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17. Sunjida Akter (Female; E-mail: [email protected])

Title: Chemical contaminants in food



Summary of work

Sunjida Akter is a 4th year project student of the group. She is now attending her theoretical class as well as project work. At the moment she is getting indirect support but does not need any direct support from ISP.

18. Uzzal Shaha (Male; E-mail: [email protected])

Title: Study of chemical pollutants in drinking water



Summary of work

Uzzal Shaha is a 4th year project student of the group. He is now attending his theoretical class as well as project work. At the moment he is getting indirect support but does not need any direct support from ISP.

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New M.S. students (Graduation in 2017)

Following six students completed B.S. Honours recently and have been promoted to MS. They will continue their theses work within few days. Their titles have yet to be confirmed. At the moment they are getting indirect support but do not need any direct support from ISP.

19. Shatabdi Roy (Female; E-mail: [email protected])



20. Md.Ahsan Habib Khandakar (Male; E-mail: [email protected])



21. Tanhaul Islam (Male; E-mail: [email protected])



22. Forkan Saroar (Male; E-mail: [email protected])



23. Elias Ahmed (Male; E-mail: [email protected])



24. Tauhidur Rahman (Male; E-mail: [email protected])



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Enclosure 1 (i)

Justification of Budget items

Equipment/ Spare parts/Service

There are two old GCs. One was bought in 2008 (GC-‐ECD, Shimadzu, 2010) and another one in 2001 (Shimadzu,-‐17A). GC-‐ECD, 2010 was more efficient and extensive worked was carried out by it. Over the last 10 years eight Ph.D., two M.Phil., 77 MS and 65 BS students worked with these GCs and organochlorine, organophosphorus and other chlorinated compounds were analyzed in different food and environmental matrices. In addition to our research work, one project from BFSLN, FAO with more than 400 samples was also completed and samples were analyzed by GC-‐ECD. Due to extensive use, this GC-‐ECD gives often trouble including unstable base line, impurities in chromatograms, fan motor changed. So the efficiency of this GC has been reduced and sometimes it becomes out of order. However, the research work of BAN: 04 is ongoing with Ph.D., M.Phil., MS and 4th year students. Other than our own research work, we have to give service to the nation and organize NITUB and ANFEC training program on GC. So it has been essential for the group to buy a new GC-‐ECD. Therefore, SEK 260,000 is requested to buy a new GC-‐ECD with auto injector in 2018 so that organohalogen and organophophorus compounds can be analyzed.

FT-‐IR is very helpful for determining functional groups and thus, identify constituents. The BAN: 04 extended work to study of different constituents in food matrix. For this FT-‐IR is a sophisticated instruments to identify different functional groups in sample. Again, it will also be used to identify microplastic. Group members of BAN: 04 were trying to buy FT-‐IR for many years but it has not been done. So SEK 180,000 is reserved in the budget of 2018 for FT-‐IR.

We are carrying out research on study of water quality parameter and it has been essential to buy Total Organic Carbon (TOC) Analyzer. TOC is an indirect measurement of organic molecules impurities present in water. SEK 280,000 is requested to buy TOC analyzer in 2019.

The group has two old HPLC which were purchased in 2001 (HPLC-‐PDA, Class VP) and 2012 (HPLC-‐PDA/Fluorescence Detector, Prominence) used for analytical and preparative purpose. Efficiency of class VP HPLC is very low as it was very old. Prominence HPLC was also connected to KOBRA cell for analysis of Aflatoxin. Ph.D., M.Phil. and MS students carried out work for analysis of chemical compounds in different types of food and environmental samples and its efficiency has been decreased. Our group is playing active role in NITUB’s HPLC training program. So a new HPLC with PDA detector and auto sampler is required to buy. Therefore, SEK 300,000 is requested to purchase HPLC with PDA detector and auto sampler in 2020.

Consumables/literature/field work

Sample collection

BAN:04 extended research work and various types of samples such as vegetables, fruits, rice, maize, wheat, fish, meats, soil/sediments, water etc. are being collected (or will be) from different parts of Bangladesh to complete the research plan of 2018-‐2020. In order to meet the expenses of transportation, buying sample collection kits, incentive to the collectors and other relevant expenses SEK 20,000, 15,000 and 20,000 have been requested in the budget of 2018, 2019 and 2020, respectively.

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Pesticide/antibiotic Standards

Three Ph.D. (male and female), one M. Phil. (female), twelve MS and eight 4th year project students are currently working in the group for analysis of contaminants in different food and environmental samples. BAN: 04 Research group also host ANFEC fellow from Laos and Cambodia as North-‐South and South-‐South collaboration. For method development & validation, different level of recovery experiments, calibration curves with different concentration of standards will be used. For this new standard of pesticides and antibiotics will be needed to purchase. Therefore, SEK 20,000 in 2018, 5,000 in 2019 and 20,000 in 2020 have been requested in the budget.

Gases for GC and GC-‐MS

Three GCs and one GC-‐MS are being used for the analysis of contaminants in food stuff and environmental samples. Helium is necessary for running the GC-‐MS and helium/nitrogen are needed for GCs. Argon gas is required as collision gas for LC-‐MS/MS. DU is giving some support for gases but that is not sufficient to run smoothly all GCs of the BAN: 04. Therefore, SEK 20,000 in 2018, 15,000 in 2019 and 20,000 in 2020 have been requested in the budget to purchase gases.

Solvents, Reagent and chemicals

To carry out research work on chemical contaminants extra pure solvents and reagents will be used. For extraction and cleanup process, anhydrous magnesium sulphate, florisil, PSA (primary-‐secondary amine), SPE Cartridges, solvent & sample filter are required to clean up of extracts. LCMS grade acetonitrile and methanol are required for analysis of samples by LCMS/MS. Some of required solvents and chemicals are being purchased from other sources but not enough. So, SEK 20,000 is requested for each year in the budget of 2018-‐2020 to purchase these items. Small apparatus

Small apparatus like micro pipettes, separating funnels, ground joint round bottomed and peer-‐shaped flasks, screw cap test tubes, bottle for solvent reservoir (LC and LCMS), vials for auto-‐sampler (GC & GC-‐MS) and common glass wares, conical flask, measuring cylinder, funnel, glass column etc. are usually used in the laboratory. Most of them are fragile and have to replace after substantial uses. To buy small apparatus SEK 20,000 is requested for each year in the budget of 2018-‐2020.

Stationaries, Computer accessories, literature etc.

Students working in the group use computer, printer and photocopy machine for data processing and storage, literature survey, and thesis, progress report & project report writing. Students use computer but most of them are old. New computer may need to buy for PhD students. A significant amount of papers are needed to support students. Moreover, GC’s and HPLC are connected to printer. Toner, virus guard and computer upgrading are also needed for students and instrument’s computer. So, SEK 15,000 is requested for each year in the budget of 2018-‐2020 for the above purposes.

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Field Trip

Last couple of years, the group members visited rural area of Bangladesh to meet real farmers at field levels and discussed about the toxic and harmful effects of pesticides and other contaminants in food and environment, managements of pesticides, their uses, dissipation etc. All these visits give extra benefit to the farmers and increase outreach activity of the project. The group felt that such kind of workshop is very fruitful and needs to be continued, and planned to arrange workshop in rural level in 2018-‐2020. Therefore, SEK 20,000, 10,000 and 20,000 are requested in 2018, 2019 and 2020 to meet the costs of field trips including interview to farmers, stockholders and survey.

Conference/Workshop

In order to disseminate the research findings of BAN:04 research group and to exchange views and ideas with other scientists it is necessary to attend international/regional seminar/symposium/conferences/workshops which are relevant to the project works. Students usually apply for travel grants to the organizer and two MS students got full support to attend SETAC Conference in Singapore in 2016. There are several international conferences/workshop on pesticides and environmental chemistry including Belfast Summit on Global Food Integrity (29-‐31 May, 2018 Belfast, UK), 12th European Pesticide Residue Workshop (22-‐25 May 2018, Munich, Germany), 47th IUPAC World Chemistry Congress (7-‐12 July 2019, Paris, France), SETAC Europe 28th Annual Meeting (13-‐17 May, 2018, Rome, Italy). SETAC Asia Pacific 2018 Conference, 16-‐19 September, Daegu, South Korea), 18th Asian Chemical Congress (2019). It would be a good opportunity for us to attend any of the event, and represent BAN:04 and meet scientists around the world and look for future collaboration. So, 50,000 SEK is requested for each year for participation at the Conference.

BAN: 04 group usually organize National Seminar on Food and Environment to disseminate research finding and exchange ideas with scientists within the country. Usually researcher from different Universities, research organization, government officials and stakeholders attend the seminar. It is planned to organize national seminar each year during 2018-‐2020 and SEK 20,000 have been requested for 2018 and 2020, and SEK 10,000 is requested for 2019 for National Seminar on Food and Environment.

Exchange visit by co-‐operating scientists

Group members of BAN: 04 are happy that Scientists from Sweden, Europe and Asia visited their laboratory. Such kinds of visit are important for developing new knowledge and renew collaborations. With our ongoing contacts, new collaborations are also established. The BAN:04 group hope that Swedish scientists and scientists from other countries will visit BAN:04 research laboratory during 2018-‐2020. SEK 50,000 is requested for each year this purpose.

Fellowship for Training/Exchange

Recently, three PhDs. and one M .Phil students joined the group. They need training to develop their skills. Students from the group visited Sweden and Korea for collaborative research and advanced training during their PhD programme earlier. New collaborations are being extended to Sweden, UK, Denmark and some other countries. Christine from Ireland and Farhan from Denmark already offered PhD students to visit their laboratories for collaborative research. We do hope that in 2019

4

and 2020 two PhD students can visit these laboratories and SEK 70,000 is requesting for each year in 2019 and 2020. Next ANFEC Training Programme on Chromatographic Techniques and Quality Assurance will be in Cambodia in 2018 and SEK 10,000 is requested for the training. Similar way, SEK 10,000 is requested for each year in 2019 and 2020 for ANFEC training. Moreover, a new collaboration is established with Asia Pacific Food Analysis Network (APFAN) who also likes to work with ANFEC. APFAN usually organize high standard Workshop on food analysis in Thailand/Philippines/Indonesia. If there will be an opportunity student can attend in 2018/2019/2020.

Support to students

Three PhDs (Shahed, Rafiza, Mizanur) and one M.Phil (Farzana), 12 MS and 14 4th Year BS project students are working in the group. New students (PhD and M.Phil) will also apply to join the group as process will start at the end of this year. PhD students usually need fellowship and Rafiza is getting fulltime fellowship. Shahed is getting salary from his job and Mizanur from other sources. MS and 4th Year BS students usually do not get financial supports or fellowship from the group but Salma Mou, one MS student getting support from the project as she is looking for LC-‐MS/MS in addition to her own research work. Students also expect some incentive after good achievement. BAN:04 also offers sometimes post doc fellow to promising students. As six Ph.D. students completed/are completing PhD they may request to carry over their work as Post Doc Fellow. One full time Research Assistant (Isteag), One part time Research Assistant (Mizan), One part-‐time Lab Assistants (Sanzida) and one part time laboratory technician (Md. Akram Hussain) have been working. Research Assistants are working for sample collection, preparation and helping students when required. Laboratory technician looks after all the instruments including GC's, GC-‐MS, HPLC and LCMS. One full time Research Assistants, one part-‐time Lab Assistants and one Technician and a cleaner (looks after sophisticated instrument rooms) need to be supported from project BAN:04. To support postgraduate students, research assistants and technician SEK 80,000 is requested for each year in the budget of 2018-‐2020.

Other costs

For audit cost SEK 15,000 is requested in the budget of 2018-‐2020. And for participation in the Reference Group Meeting in 2020 SEK 15,000 is requested in the budget of 2020.

Logical Framew

ork Matrix

In th

e matrix

you

insert th

e long-‐term overall ob

jective of you

research activity

as w

ell as y

our spe

cific objectiv

es, as s

tated in Enclosure 1. You

also

fill ou

t ou

tputs a

nd outcomes fo

r each specific ob

jective with

indicators m

easurin

g the prog

ress and

perform

ance, as w

ell as a

ssum

ptions, sou

rces of d

ata an

d the

strategy to

collect it. The

template is partly

pre-‐filled

at the

Outcome an

d Pe

rforman

ce in

dicator level, to prov

ide yo

u with

an exam

ple. This can be

chan

ged or sub

stitu

ted as you

see

fit.

Overall ob

jective (Im

pact): To

improve the status of foo

d safety and

qua

lity, and

redu

ce enviro

nmen

tal con

tamination in Ban

glad

esh by th

e grou

p stud

ies

of organ

ic pollutants in food

and

enviro

nmen

t

Type

s of Outpu

ts O

utcomes

Pe

rforman

ce In

dicator o

f Outcome

Data So

urce D

ata Co

llection

Strategy

Assumptions

Specific Objectiv

e 1: To increase existing labo

ratory fa

cilities a

nd th

e prod

uctio

n, qua

lity an

d relevance of sc

ientific results

Offe

ring po

st

grad

uate program

Master

and

PhD

stud

ents

grad

uate from

the prog

rams

Num

bers of m

aster’s and

doctoral the

ses d

efen

ded,

numbe

r of p

ublications

Registratio

n,

Subm

ission

of

dissertatio

n,

Presen

tatio

n

Repo

rt from

the

depa

rtmen

t, supe

rviso

r, External

exam

iner

Degree

awarde

d an

d nu

mbe

r of

publication

Procurem

ent

of

new equ

ipmen

ts Pu

rcha

se and

installatio

n of

new equ

ipmen

ts like FTIR,

GC-‐EC

D, AAS

, LC-‐MS/MS, LC

and GC

Num

ber o

f instrum

ent Installed an

d users training,

Performan

ce of the

instrumen

t,

Mainten

ance of stock re

gistrar, vouche

r, Ph

ysical

investigation

From

labo

ratory,

quotation,

Challan,

Delivery,

Installatio

n certificate

Physical

verification.

Quo

tatio

n, Bills,

Paym

ent,

Cheq

ue,

Installatio

n certificate,

Ope

ratio

n certificate

Supp

ort from

UGC,

University

, ISP,

EU and

Governm

ent

Establish

ing

exchan

ge visit

Collabo

ratio

n with

scientists

region

ally and

in th

e North

Num

ber a

nd duration of excha

nge visits, num

ber o

f stud

ents traine

d with

partners, and

num

ber o

f co-‐

publications

Visa,

Immigratio

n stam

ped,

Ticket,

Boarding

pa

ss

Photo, Host

institu

te re

port, Establish

men

t of

collabo

rativ

e research,

Journa

l during

joined

pu

blication

Dissem

ination of

research re

sults

Use of research results from

supp

orted activ

ities fo

r de

velopm

ent

Num

ber o

f paten

ts, n

umbe

r of p

ublications, n

umbe

r of

meetin

g with

policy makers, num

ber o

f interview

to th

e med

ia.

Publication

lists, m

eetin

g minutes,

Publish

ers

record

BFSA

Offe

ring

instrumen

tal

training

Participation of M

aster, Ph

D stud

ents and

you

ng fa

culty

staff.

Num

ber o

f Master, Ph

D stud

ents ,fem

ale pa

rticipan

ts and

youn

g faculty

staff a

tten

ded an

d nu

mbe

r of certificate

distrib

ution

Registratio

n,

certificate,

etc.

Certificate from

Training

prog

ram

Participation at

the training

Prop

er

man

agem

ent o

f fund

Fund

ing is well m

anaged

, used

and

repo

rted

, scientifically and

econ

omically

Fulfilm

ent o

f bud

get, tran

sparen

cy and

correctne

ss of

local accou

nt, com

pleten

ess o

f scien

tific re

porting

Cheq

ue boo

k,

bank

statem

ent,

audit

Repo

rt from

the

audit

Specific Objectiv

e 2: To Iden

tify an

d qu

antify chem

ical con

taminan

ts in fo

od stuff a

nd enviro

nmen

t

Carry ou

t research

work

Mod

ified

existing metho

ds,

Develop an

d validate of new

metho

ds, Produ

ction an

d pu

blication of

Research re

sults re

levant to

research activity

Num

ber o

f sam

ple collection

Num

ber o

f sam

ple an

alyzed

,

Logb

ook

maintain,

Maintaining

records b

y an

alyzer and

grou

p lead

er/dep

uty lead

er

Activ

ities

with

the

Logb

ook

maintain,

Data collection

and record,

Exchan

ge visits

of sc

ientists,

Verification/cr

oss c

heck by

the second

pe

rson

of the

grou

p

collabo

rativ

e scientists

stud

ents, etc.

Sp

ecific Objectiv

e 3: To create awaren

ess a

bout sa

fe use of che

mical com

poun

ds

Worksho

p with

farm

ers a

t rural

area

Upg

rade

of kno

wledg

e of

farm

ers, increase of the

ir confiden

ce, interview

to

farm

ers, stakeh

olde

rs, survey

etc.

Involvem

ent o

f local autho

rity

Interview fa

rmers, su

rvey

Prog

ram list

Survey list

Documen

tatio

n,

Photog

raph

y Supp

ort from

local peo

ple,

University

, ISP

Seminar/w

orksho

p in urban

area

Recommen

datio

n for

man

agem

ents and

policy

making grou

ps

Num

ber o

f participan

ts

Num

ber o

f lecture delivered

Prog

ram list

participan

t attend

ance

sheet

Documen

tatio

n,

Photog

raph

y Supp

ort from

UGC,

University

, ISP

Specific Objectiv

e 4: To attract m

ore female stud

ents and

improve the pa

rticipation of w

omen

in th

e research program

Enrolm

ent o

f female stud

ents

Female stud

ents enrol fo

r MSc/PhD

in th

e grou

p Num

ber a

nd sh

are of fe

male stud

ents enrolling at M

Sc,

PhD level in the grou

p Ad

miss

ion in

the

university,

participation

in th

e seminar/w

orksho

p

Stud

ents’ record

of th

e un

iversity,

Attend

ance

sheet o

f seminar/w

orksh

op

University

, ISP

1

Enclosure 3 Application for Improving Gender Balance

1. Contact info. Program (Physics/ Chemistry/ Mathematics) CHEMISTRY

Research Group code BAN:04

1) Leader (Research Group leader /Network coordinator: title,given name, family name) PROFESSOR MOHAMMAD SHOEB

Address: Department/ unit :Department of Chemistry, University/institute: University of Dhaka Street (visiting address): Curzon Hall, Post/ Zip code: 1000 City: Dhaka Country: Bangladesh E-mail address (es): [email protected];[email protected] Website: www.du.ac.bd Telephone Office +88029661920-82

Home +8802-9124617

Mobile +8801715191988

Office +88029667222

2. Introduction

Women constitute around 50% of the world population with men and are anticipated to participate proportionally to their share of the population in a consequence of gender balance. In most countries around the world, however, women participate less than what would be expected based on the gender distribution in the population. Iceland, Norway, Finland, Sweden and Ireland are the highest ranked countries about gender equality while Sweden is number one in in EU. Bangladesh has topped the South Asian countries in gender equality & ranked 72nd among 144 countries. We are now changing our minds slowly but surely & unlike earlier days when they stayed at home only and do only kitchen stuffs, nowadays, they roam outside and also earns money like the male members of the society which also leads to more economic benefits not to the individuals but to the society as well. It has been found that around 15.5% of girls gets no education, only 10.4% completes primary education and overall 20% manages to complete secondary educations. Our mothers are our teachers for every passage of life. It is a mother who has the power of not only rising her child but also the power to correct the wrong in her child. So, if we educate the mother, we educate the whole generation. Moreover, as women are the half of the population it is very important to educate them for the social, economic developments of the country. Recently women are participating in every sector in Bangladesh, such as- politics, education, socio-economic, science, and technology. So women need to be encouraged for higher study for development of country.

3. Project/ activity description and time frame of the activities.

During 2015-2017, among eleven staffs, nine were male and two female were in the group while during 2018-2020 these are 6 male and 3 female. The group always plan to keep balance but sometimes change if anybody goes for leave or deputation. In case of students, out of 30, 12 were female students last time. In future granting period it may be more balance between male and female students.

Gender distribution has powerful potential to improve our economy, security and the overall well-being of the population. Studies have found that countries with less gender inequality

2

are more secure, and peace agreements last longer when women are at the negotiating table. Women need to come forward to every sector in Bangladesh and the situation is improving. Women are in different positions in Bangladesh now and they are contributing for the development. We strongly feel that gender distribution should be equal and contributions of women are important factor for the development of a country. Gender equality sometimes looks like an impossible task—a pursuit without an end. But we can make progress, and that progress is worth making. Little by little, discussion by discussion, step by step, we can improve the lives of women and girls, men and boys with little help and support. And in doing so, we can reach our shared goals of peace, prosperity, and security. In this issue, our strategy is to maintain a balance between male & female students in giving training & in other opportunities. Therefore, we always encourage our female students to develop their skill and learn new techniques and attend training programs for their better career. To reach our desired goal ISP could be our incalculable support by offering more fellowships and training programs.

University of Dhaka believes in gender equity in every aspects. Students are admitted to the University absolutely based only on their merit rather than considering their gender. For undergaraduate studies, marks obtained in entrance examination and their school & high levels results are the criteria for admission. Male and female students have equal oppotunity for higher studies in the university. Same rule is applicable for recruitment of faculties in all departments of Dhaka University. University has 6 hall of residence for women stduents accommodation. Both male and female students and staffs have swimming, gymnesium facilities. So all policy are documented.

a) Expected results from the activity

Our main objective is to attract post graduate students for their degree and develop their skills by providing adequate training at home and abroad. As women has lack opportunity in Bangladesh we wish to provide monthly fellowship each year of their PhD program and arrange training or allow to participate Conference. By this activity, women will be educated and contribute for the development of the society and country.

BUDGET

Specification of costs in 2018-2020 Specification of costs 2018 (SEK) 2019 (SEK) 2020 (SEK) S for PhD candidate

SEK 2,000 per month for 12 months 24,000 24,000 24,000

Instrumental Training or participation at Conference/ Seminar (International/ Local)

6,000 6,000

6,000

Total Costs (SEK)

30,000 30,000 30,000

Grant Total (2018-2020) 90,000

Justification of budget

One PhD (Rafiza) and One M.Phil (Farzana), two MS (Salma, Farhana) and 3 BS (Shatabdi, Tahmina, Sunjida) female students are working in the group now. The number will also increase in the following years. MS and BS students do not need fellowship but -PhD and

3

M.Phil students are matured students and they need fellowship. Without fellowship it would be impossible for them to support themselves. In the gender balance improving programme we are applying for Scholarship to PhD students with fellowship for an amount of SEK 20, 000 per month for 3 years and 6,000 per year for participation of female students at conference/seminar or training programme abroad.

1

Curriculum Vitae Name: Dr Mohammad Shoeb Position: Professor Address: Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh. Phone: 880-2-9661920 Ext. 7143 (office), 88-02-8051390 (Home) Mobile: 880-1715191988, E-mail: [email protected]; Web : www.du.ac.bd Duties/Activities: Teaching, and Research on Studies of Pesticides & Persistent Organic Pollutants (POPs) and Antibiotics in Food and Environmental samples and Bioactive Natural Products. Previous Employment Associate Professor:2009-2013 Assistant Professor: 2006-2009 Lecturer: 2000-2006 Scientific Officer at Bangladesh Council of Scientific and Industrial Research (BCSIR) (1998-2000). Academic qualifications

• PhD in Chemistry, The School of Pharmacy, The Robert Gordon University, UK (15 Dec 2005)

• MSc in Chemistry (Natural Products Chemistry), University of Aberdeen, UK ( 2003)

• MSc in Organic Chemistry (Natural Products Chemistry), University of Dhaka, Bangladesh (1997)

• BSc (Hons) in Chemistry, University of Dhaka, Bangladesh (1995) Post Doc: Linkoping Univ, Sweden on Environmental Chemistry and Sustainable Development (October 2014) University of Aberdeen on marine bio-diversity (October –December 2012). Visiting researcher: Department of Environmental Chemistry, Stockholm University,

Sweden (01 Oct-18 Dec 2008). Areas of Expertise: Organic Chemistry, Natural products chemistry, Analytical

Chemistry, Food and Environmental Chemistry Research Leadership I am a group leader of Research group consisting of 35 members including faculty members, Ph.D, M.Phil. MS. BS (4th year projects students), Research assistants, Research Fellow and visiting fellow. I have dynamic leadership capability for motivating and managing research team, organizing and managing events, planning for research and applying for research grants. I have strong collaboration with local and International scientific community. I keep records of all our activities and improve for sustainability.

2

Some of involved projects are below: 1) Acting as a Leader of IPICS supported research project in Bangladesh, “Studies of Organic Pollutants in Food and Envirn., BAN:04 since 2015 and worked as Deputy Leader from 2003 and potential member from 20036; 2) Acting as a Deputy Leader of World Bank supported and University Grant Commission, Bangladesh coordinated HEQEP project “Chemical Contaminants in Food and their Effect on Human Health, 3) Acted as Project Leader of IFS supported research project “Isolation of biologically active secondary metabolites from endophytic fungus”, 4) Project Leader of research project “Anticancer drugs from endophytic fungus” supported by Ministry of Education, Government of Bangladesh 5) Project Leader of research project “Marine microplastic in the Sundarban” supported by Ministry of Science and Technology, Government of Bangladesh 6) Co-applicant of International Project from Swedish Research Link (SRL) “Marine debris and microplastic in the Sundarban” 7) Completed several small projects as Principal Investigator supported from University. Networking activity Working as a Head of Swedish Institute Regional Network “Sweden Alumni Network in Bangladesh” Working as Convener of Chemistry Olympiad in Bangladesh where 10,000 students participants Working as a member from inception of IPICS supported Network ANFEC (Asian Network of Research on Food and Environment Contaminants) and playing active role for different activities Member of IPICS supported Network NITUB (Network of Instrument Technical Personnel and User scientists in Bangladesh) and playing active role in different events Member of IPICS supported Network ANRAP (Asian Network of Research on Anti-diabetic Plants) Participated OPCW supported meeting of Networking in Qatar and China.

3

Membership: Affiliated Member of IUPAC, The Royal Australian Chemical Institute, The Phytochemical Society of Europe, Bangladesh Chemical Society (LF-1074)-National Adhering organization of IUPAC, Member NITUB. Award received

• Swedish Institute Guest Researcher Award 2014 for Post Doctoral work • Common Wealth Academic Staff Fellowship UK Award 2012 for Post

Doctoral work • Young Scholar Award 2010 from American Chemical Society to attend

Pacifichem congress 2010, 15-20 Honolulu, Hawaii, US • IUPAC Young Chemist Award 2009 to attend 42nd IUPAC Congress,

August, Glasgow, UK • IUPAC Young Chemist Award 2007 to attend 41st IUPAC Congress, August,

Italy • Phytochemical Society of Europe travel grant to attend a conference in Poland,

2004 • The Robert Gordon University Award for PhD Research, UK (2000-2005) • Science and Technology HRD Award, Ministry of Science & Technology,

Government of Bangladesh, 2001 • UNESCO travel grant to visit Karachi, Pakistan, 2001

Courses/workshop attended:

• General Awareness on Hazardous Chemicals, Bangladesh National Authority for chemical Weapons Convention (BNACWC), Dhaka, Bangladesh, 27 July, 2011.

• OPCW Workshop on Article X1, The Hague, The Netherlands 24-25 November 2010.

• Analytical Skill Development Course, DELFT, The Netherlands, July, 2007 organized by OPCW

• Course of the enhancement of laboratory skills in using LCMS to analyse Chemicals related to the chemicals weapons convention, Finland, March, 2007 organized by OPCW

• BAS-INASP Author AID Research Writing Skills Workshop, Bangladesh, May, 2009

• Workshop on Chemical Hazards and Safety organized by Bangladesh Chemical Society, Bangladesh, August, 2008

• Attended Pesticides workshop in Saskatoon, Canada, June, 2007 • Off-Licence Certificate (ServeWisePlus) Course by ServeWise and Scottish

Qualification Authority, UK, December, 2005 • Research Method Course, Teaching and Demonstrating, RGU, UK, April,

2004 • HEJ-COMSAT Workshop on the use of Spectroscopy Techniques in

Structural Organic Chemistry, Karachi, Pakistan, Feb. 14-21, 2000 • Regional Training Program on Spectroscopy , ITT Madras, India, Jan. 01-13,

2001.

4

Research Activities: i) Experiences:

Major skills are described below:

• Well trained in extraction of plant materials, marine organisms and

cyanobacteria, endophytci fungi and environmental samples • Separation of extract using different types of chromatographic techniques e.g.

normal and reversed phase column, TLC, VLC prep-TLC and HPLC • Trained in spectroscopy e.g. 1D and 2D NMR, UV, IR and mass spectroscopy • Trained in LC, LC-MS, LC-MS/MS, GC, GC-MS and other analytical

techniques Country visited: Australia, Austria, Belgium, Canada, China, Greece, France, Finland, Germany, Laos, Malaysia, Nepal, India, Italy, Pakistan, Poland, Qatar, South Korea, Singapore, Switzerland, Tanzania, Sweden, Thailand, The Netherlands, Turkey, UK, United Arab Emirates, USA, Latvia. Publications: 76 (1) Mohammad Shoeb, Md. Amjad Hossain, Md. Golam Kibria, Tonima Mustafa and Nilufar Nahar, Department of Chemistry, University of Dhaka, Dhaka Bangladesh 1000. Bioaccumulation of Mercury in Fish Species from Different Tropic Level, Journal of Food Science and Engineering (2017) 17-28 (2) Hasan M K, Khan A H, Sultana A, Shoeb M and Nahar N. Evaluation of Diclofenac by UV-Vis Spectrophotometer in some locally produced Tablets. Dhaka University Journal 2017 65(2),163-16. (3) Mohammad Shoeb, Prithu Mondal, Henrik Kylin and Nilufar Nahar, Chemical and Biological Activity of Mushrooms Naturally Occurring in Bangladesh, The Asian Journal of Pharmacognosy, 2017, 1 (3):42-51. (4) Shoeb M, Al Mamun M S, Noor R E, Al Mahmud M I R, and Nahar N, Residual DDTs and fatty acid compositions in popular two fish samples, Dhaka Univ. J. of Sci., 2017, 65 (1) 77-80.

(5) Munia, Z. S, Shoeb M, Kamruzzaman M, Mamun M I R and Nahar N, Determination of the Toxicity Level of Imidacloprid in Rice sample Produced in Bangladesh J Bang. Chem. Soc 2016, 28 (1 & 2) 1-4.

(6) A. Sultana, M. A. H.Sakib, M. G. Kibria, M. R. Islam, Shoeb M and N. Nahar, An easy and cheaper method to assay paracetamol and metronidazole. J. Bang. Chem. Soc, 2016, 28 (1 & 2): 5-11.

(7) Mou S A, Akther T, Shoeb M, Sultana A and Nahar N, Studies of preservative and coloring agent in jam and jelly, Dhaka Univ. J. of Sci., 2017, 65 (1) 83-84.

5

(8) Khan S H, Matin M, Sultana N, Mosihuzzaman M, Ali, L Rokeya, B.. Khan A K A, Shoeb M. and Nahar N. Hypoglycemic activity of Scoparia dulcis L. extract and isolation of flavonoid glycosides, J Bang. Chem. Soc, 2016, 28 (1 &2) 1-4

(9) Hossain M. A, Shoeb M. and Nahar N., DDT and Its Metabolites in Fresh Water Fish Samples J. Food Sci. and Engineering 2016, 6, 344-350. (10) Shoeb M, Mahim A, Mamun MIR, Nahar N, Organochlorine pesticide residues in poultry meats of Bangladesh Croat. J. Food Sci. Technol. 2016, (8 (1) : 10.17508/CJFST.2016.8.1.04 (11) Nahar, N Khan WI and Shoeb M, Physico-Chemical Analysis and Composition of Camel Milk of Bangladesh, J. of Basic & Appl. Sci., 2016, 12, 231-235 (12) Nahar N, Hossain M M, Uddin-Al-Mahmud, M N, Shoeb M, Latifa G A and Kabir, K H Dissipation of Cypermethrin in Bean and Cauliflower, Dhaka Univ. J. Sci, 2016, 64(1):89-90 (13) Sultana, A Islam R, Islam M M, Shoeb M and Nahar N, Study of Preservatives and Stimulants in Commercial Soft Drinks, J. Bangladesh Pharm.Soc. 2016, 19 (1): 89-95. (14) Shoeb M, M Khondker, N Nahar, A new steroidal glycoside from Corypha taliera Roxb., a globally endangered species, Nat Prod Res. 2016, 30(3); 281-5. (15) Abida Sultana, Md. Sharear Saon, Mohammad Shoeb and Nilufar Nahar, Evaluation of Vitamin B1, B2 and B6 Tablets in Bangladesh by UV-Vis Spectrophotometer, Indian Journal of Pharmaceutical and Biological Research, 2016, 4 (4), 32-38. (16) M N U Al Mahmud, F Khalil, M M Rahman, M I R Mamun, Shoeb M, A M Abd El-Aty, Jong-Hyouk Park, and Ho-Chul Shin, Analysis of DDT and its metabolites in soil and water samples obtained in the vicinity of a closed-down factory in Bangladesh using various extraction methods, Environ Monit Assess, 2015,187:743 (17) Mandal S, Khuda N, Mia M R, Moniruzzaman M, Nahar N, Shoeb M and Mamun, MIR 2014, Analysis ofground and surface water samples from some areas of Dhaka city for Poly Aromatic Hydrocarbons (PAH), Dhaka Univ. J of Sci., 2015, 63(2):73-76 (18) Sultana A, Hossain M R., Mamun M I R, Shoeb M,and Nahar N. Compositions of Rice Bran Oil in some selected Bangladeshi paddy samples.Journal of Bangladesh Chemical Society, Vol.27 (1&2), 33-37, 2015.

(19) Shoeb M, M. S. U. Khandaker, and N. Nahar, Oxygenated Secondary metabolites from Endophytic Fungi Isolated From Ocimium sanctum, Journal of Basic & Applied Sciences, 2015,11, 434-439.

6

(20) Nahar N, Sharma H M, Shoeb M, Mamun M I R and Sarker M L, Dissipation Pattern and Residue of Fenvelerate in Tea of Bangladesh. 2015, Dhaka Univ. J. Sci, 2014, 63 (2), 73-76

(21) Shoeb M, Chemical and Biological Studies of Cyanobacteria., Dhaka Uni. J. Pharm. Sci, 2014, 13(2), 119-124.

(22) Shoeb M, A steroid from the brown algae Platythalia angustifolia, Journal of Bangladesh Chemical Society, J Bang. Chem. Soc. 2014, 27 (1&2), 38-41.

(23) Mozammel, H, Shoeb M and Nahar N, Studies on Chemical Constituents and Biological Activities of an Endophytic Fungi from Magnifera indica L., Dhaka Uni. J. Pharm. Sci, 2014, 13(1), 63-67.

(24) Shoeb M, Mozammel, H, and Nahar N, Bioactive compounds from endophytic fungus Penicillium thiomii isolated from Terminalia chebula Retz, J. Nat. Prod. Plant. Resour. 2014, 4(3) 65-70. (25) Sultana A, Hossain MR, Mamun MIR, Shoeb M and Nahar N, Composition of rice bran oil in some Bangladeshi paddy samples. J Bang. Chem. Soc. 2014, 27 (1&2), 32-37

(26) Shoeb M, Hoque M E, Thoo-Lin P K and Nahar N, Anti-pancreatic cancer potential of secalonic acid derivatives from endophytic fungi isolated from Ocimum basilicum, Dhaka Uni. J. of Pharm. Sci., 2013, 12 (2), 91-95.

(27) Shoeb M, Nusrat S E and Khondker M, Chemical investigation of Corypha taliera Roxb, Bangladesh Journal of Botany, 2013, 42 (1): 51-53.

(28) Shoeb M, Hasan Z, Saha N K, Karim MM, Nahar N,Antimicrobial activity of carbazole alkaloids from Murraya koenigii(L) Spreng leave Int. J. Med. Arom. Plants, 2013, (ISSN 2249-4340) 3 (2), 131-135 (29) Sultana A, Haque M S, Shoeb M, Islam S M I, Mamun M I R and Nahar N, Presence of Yellow 6, an Artificial Color Additive in Orange JuiceJ Bang. Chem. Soc., 2012, 25 (1), 80-86

(30) Hossain M S, Sokeng S, Shoeb M, Hasan K, Mosihuzzaman M, Nahar N, Ali L, Khan A Azad, Rokeya B, Hypoglycemic effect of Irvingiagabonensis (Aubry-Lacomate Ex. Ororke) Bailon in Type 2 diabetic Long-Evans rats; Dhaka Univ. J. Pharm. Sci., 2012, 11 (1), 19-24

(31) Nahar N, Shoeb M, Mamun M I R, Ahmed S, Hasan M M and Kabir A, Studies of dissipation pattern of cypermethrin in tomato, J Bangladesh Chem. Soc., 2012, 25 (2), 200-203

(32) Mandal S, Rahman A M H, Mamun MIR, Shoeb M and Nahar N, Method Development and validation for estimation of Commercially produced sex pheromones in Lure, J. Bangladesh Chem. Soc., 2012, 25 (2), 180-185

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(33) Shoeb M, Thoo-Lin P K, Nahar N, Anti-colon cancer activity of endophytic fungal strains from Terminalia chebula Rezt, Bangladesh J Pharmacol., 2012, 7(1), 47-49

(34) Zamir R,Hossain M, Shoeb M, Mosihuzzaman M and Nahar N, Organochlorine pesticides in three fish samples, The Dhaka Univ. J. Sci., 2013, 61(2) 216-16

(35) Linderholm L, Jakobsson K, Lundh T, Zamir R, Shoeb M, Nahar N, and Bergman Å,Environmental exposure to POPs and heavy metals in urban children from Dhaka, Bangladesh J. Environ Monit, 2011, 13 (10), 2728-34 (36) Shoeb, M., Nahar N, Ahmed F, Mosihuzzaman M and Nahar N, , Studies on locally available three anti-diabetic herbal medicines, Bangladesh J Pharmacol, 2011, 6, 124-127.

(37) Shoeb, M., Begum, S., Mamun, M.I.R. and Nahar, N., Studies on chemical and Biological activity of endophytic fungus from Terminalia chebula Retz, Journal of Bangladesh Chemical Society, 2010, 23 (1 & 2), 98-104.

(38) Shoeb, M., Begum S and Nahar N, Study of an endophytic fungus from Aquilaria malaccensis Lamk, Bangladesh J Pharmacol, 2010, 5, 21-24

(39) Shoeb, M., Nahar, N., Mandal, S, Sarma, H., M. and Seraji, M., S., I., Nitrofuran antibiotics and organochlorine pesticides in Fish and Poultry Fish, Feed ingredients, and water samples collected from shrimp cultivation area, Journal of Bangladesh Chemical Society, 2009, 22 (1), 1-8. (40) Shoeb, M., MacManus, S. M., Kong-Thoo-Lin, Celik S. Jaspars M., Nahar, L. and Sarker, S. D., Bioactivity of the extracts and isolation of lignans and a sesquiterpene from the aerial parts of Centaurea pamphylica (Asteraceae), DARU, 2007 15, 3, 118-122. (41) Shoeb, M., MacManus, S. M., Jaspars, M., Kong-Thoo-Lin, P., Nahar, L., Celik, S. and Sarker, S. D. Bioactivity of two Turkish endemic Centaurea species, and their major constituents, Brazilian Journal of Pharmacognosy, 2007 17 (2), 155-159. (42) Shoeb, M., Celik, S., Nahar, L., MacManus, S. M., Kong-Thoo-Lin, P., Jaspars, M., and Sarker, S. D. Two salonitenolide derivatives from the aerial parts of Centaurea gigantea inhibit the growth of colorectal cancer cells in vitro, Natural Products Communications, 2007, 2 (2), 121-125. (43) Uddin, S. J., Nahar, L., Shilpi, J. A., Shoeb, M., Borkowski, T., Gibbons, S., Middleton, M., Byres, M. and Sarker, S. D. Gedunin, a limonoid from Xylocarpus granatum inhibits the growth of CaCo2 colon cancer cell line in vitro, Phytotherapy Research, 2007, 21, 757-761.

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(44) Uddin, S. J., Shilpi, J. A., Byres, M., Middleton, M., Shoeb, M., Nahar, L. and Sarker, S. D. Swarnalin and cis-swarnalin, two new tetrahydrofuran derivatives with free radical scavenging activity, from the aerial parts of Cuscuta reflexa, Natural Products Research, 2007, 21 (7), 663-668. (45) Sarker S. D, Shoeb M, Celik S, Jaspars M, Nahar L, Extracts of Centaurea bornmuelleri and Centaurea huber-morathii inhibit the growth of colon cancer cells in vitro. Oriental Pharmacy and Experimental Medicine 2007 7(4), 336-340. (46) Nazemiyeh, H., Maleki N. Mehmani F, Kumarasamy Y, Shoeb M., Garjani A. Sarker S. D. Assessment of anti-inflammatory properties of ethyl acetate extract of Stachys schtschegleevii Sosn., DARU 2007, 15, 4. (47) Shoeb, M., MacManus, S. M., Jaspars, M., Nahar, L., Kong-Thoo-Lin, P., Celik, S. and Sarker, S. D., Lignans and flavonoids from the seeds of Centaurea bornmuelleri Hausskn. Ex. Bornm. and Centaurea huber-morathii Wagenitz., Polish J. Chemistry, 2007, 39-44. (48) Shoeb, M., Jaspars, M., MacManus, S. M., Celik, S., Nahar, L., Kong-Thoo-Lin, P., and Sarker, S. D., Anti-colon cancer potential of phenolic compounds from the aerial parts of Centaurea gigantea (Asteraceae), Journal of Natural Medicine, 2007 61:164-169. (49) Shoeb, M., Anticancer agents from medicinal plants, Bangladesh Journal of Pharmacology, 2006, 35-41.

(50) Shoeb, M., Jaspar, M., MacManus, S. M., Celik, S., Kong-Thoo-Lin, P. and Sarker S. D., Bioactivity of the extracts and the isolation of lignans from Centaurea dealbata, ARS Pharmaceutica, 2006, 47 (4), 417-424. (51) Shoeb, M., MacManus, S. M., Kumarasamy, Y., Jaspar, M., Nahar, L., Kong-Thoo-Lin, P., Nazemiyeh, H. and Sarker, S. D. Americanin, a bioactive dibenzylbutyrolactone lignan, from the seeds of Centaurea americana, Phytochemistry, 2006, 67, 2370-2375.5 (52) Shoeb, M., MacManus, S. M., Jaspars, M., Trevidadu, J., Nahar, L., Kong-Thoo-Lin, P., and Sarker, S. D., Montamine, a unique dimeric indole alkaloid, from the seeds of Centaurea montana (Asteraceae), and its in vitro cytotoxic activity against the CaCo2 colon cancer cells, Tetrahedron, 2006, 62, 11172-11177. (53) Delazar, A., Modarresi, M., Shoeb, M., Nahar, L., Reid R. G., Majinda, R. R. T. and Sarker, S. D., Eremostachiin: A new furanolabdane diterpene glycoside from Eromostachys glabra, Natural Product Research, 2006, 20, 167-172.

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(54) Nazemiyeh, H., Shoeb, M., Movahhedin, N., Kumarasamy, Y., Talebpour, M., Delazar, A., Nahar, L. and Sarker S. D., Phenolic compounds and their glycosides from Stachys schtschegleevii (Lamiaceae), Biochem. Syst. Ecol., 2006, 34, 721-31 (55) Shoeb, M., Celik, S., Jaspars, M., Kumarasamy, Y., MacManus, S., Nahar, L., Kong, T. L. P., Sarker, S. D., Isolation, structure elucidation and bioactivity of schischkiniin, a unique indole alkaloid from the seeds of Centaurea schischkinii. Tetrahedron, 2005, 61, 9001-9006. (56) Dey, S K, Shoeb, M., Rob, T., Nahar. N., Mosihuzzaman, M. and Sultana, N, Biological and Chemical Studies on Calycopteris floribunda Lamk leaves The Dhaka University Journal of Pharmaceutical Sciences. 2005, 4 (2), 103-106. (57)Shoeb, M., Mamun, M. I. R, Nahar. N and Mosihuzzaman, M., Biological screening of Zizyphus rugosa and Zizyphus oenoplia extractives. The Dhaka University Journal of Pharmaceutical Sciences, 2005, 4(2), 131-134. (58) Rahman, M. R., Ara, S., Shoeb, M., Nahar. N and Mosihuzzaman, M., Isolation free radical scavenging agents from the leaves of Zizyphus rugosa Linn. Journal of the Bangladesh Chemical Society, 2005, 18(2), 1-6. (59) Nahar, L., Russell, W. R., Middleton, M., Shoeb, M. and Sarker, S. D., Antioxidant phenylacetic acid derivatives from the seeds of Ilex aquifolium, Acta Pharmaceutica, 2005, 55, 187-193. (60) Middleton, P., Stewart, F., Al-Qahtani, S., Egan, P., O’Rourke, C., Abdulrahman, A., Byres, M., Middleton, M., Nahar, L., Shoeb, M., Delazar, A., Kumarasamy, Y. and Sarker, S. D., Antioxidant, antibacterial activity and general toxicity of Alnus glutinosa, Fraxinus excelsior and Papaver rhoeas, Iranian Journal of Pharmaceutical Research, 2005,2, 81-86. (61) Delazar, A., Gibbons, S., Kumarasamy, Y., Nahar, L., Shoeb, M., Sarker, S. D., Antioxidant phenylethanoid glycosides from the rhizomes of Eremostachys glabra ( Lamiaceae). Biochemical and Systematic Ecology, 2005, 33, 87-90. (62) Sarker, S. D., Kumarasamy, Y., Shoeb, M., Celik, S., Yucel, E., Middleton, M. and Nahar, L., Antibacterial and antioxidant activities of three Turkish species of the genus Centaurea, Oriental Pharmacy and Experimental Medicine, 2005, 5, 246-250. (63) Shoeb, M., Jaspars, M., MacManus, M. S., Majinda, R. T. R., Sarker, S. D., Epoxylignans from the seeds of Centaurea cyanus (Asteraceae). Biochemical Systematic and Ecology, 2004, 32, 1201-1204.

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(64) Shoeb, M., Rahman, M. M., Nahar, L., Delazar, A., Jaspars, M., MacManus, S., Sarker, S. D., Bioactive lignans from the seeds of Centaurea macrocephala, 2004, DARU 12, 87-93. (65) Delazar, A., Byres, M., Gibbons, S., Kumarasamy, Y., Modarresi, M., Nahar, L., Shoeb, M., Sarker, S. D., Iridoid Glycosides from Eremostachys glabra. Journal of Natural Products, 2004, 67, 1584-1587. (66) Egan, P., Middleton, P., Shoeb, M., Byres, M., Kumarasamy, Y., Middleton, M., Nahar, L., Delazar, A and Sarker, S. D., G15, a dimer of oleoside, from Fraxinus. Biochemical and Systematic Ecology, 2004, 32, 1069-1071. (67) Kumarasamy, Y., Byres, M., Cox, P. J., Delazar, A, Jaspars, M., Nahar, L., Shoeb, M., and Sarker, S. D., Isolation, structure elucidation, and biological activity of flavone C-glycosides Alliaria petiolata. Chemistry of Natural Compounds, 2004, 40, 122-128. (68) Muller, M., Byres, M., Jaspars, M., Kumarasamy, Y., Middleton, M., Nahar, L., Shoeb, M., and Sarker, S. D., 2D NMR spectroscopic analyses of archangelicin from the seeds of Angelica archangelica, Acta Pharmaceutica, 2004, 54, 277-285. (69) Delazar, A., Shoeb, M., Kumarasamy, Y., Byres, M., Nahar, L, Modarresi, M. and Sarker, S. D. Two bioactive ferulic acid derivatives from Eremostachys glabra, DARU, 2004, 12, 49-53. (70) Murphy, E. M., Nahar, L., M., Byres, Shoeb, M., Siakalima, M., Rahman, M. M., Gray, A. I. and Sarker, S. D., Coumarins from the seeds of Angelica sylvestris (Apiaceae) and their distribution within the genus Angelica, Biochem. Syst. Ecol., 2004, 32, 203-207. (71) Shoeb, M. and Jaspars, M., Chlorinated C12 Fatty Acid Metabolites from the Red Alga Gracilaria verrucosa, J. Nat. Prod. 2003, 66, 1509-1511. (72) Cox, P. J., Kumarasamy, Y., Nahar, L., Sarker, S., Shoeb, M., Luteolin. Acta Cyrstallographica, 2003, E 59, o975-o977. (73) Cox, P. J., Jaspars, M., Kumarasamy, Y., Nahar, L., Sarker, S and Shoeb, M., A mixed crystal of imperatorin and phellopterin, with C-H...O, C-H...π and π-π interactions. Acta Cyrstallographica, 2003, C59, 0520-0522. (74) Nahar, N., Hossain, M. A., Aziz, M. A., Das, R. N., Khan, S. H., Shoeb, M. and Mosihuzzaman, M. Studies of Oxalis corniculata Linn, J. Bangladesh Chem. Soc. 2001, 14, 213-218.

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(75) Rahman, M., Shoeb, M., Nandi, N. C., Alamgir, M. and Husain, M., Introduction of Patchouli, Pogistemon cablin (Blanco) Benth: An Essential Oil Bearing Plant in Bangladesh. Bangladesh Journal of Scientific and Industrial Research. 2001, 36 (1-4), 14-18. (76) Nahar, N., Das, R. N., Shoeb, M., Marma, M. S., Aziz, M. A. and Mosihuzzaman, M. Four Triterpenoids from the bark of Zizyphus rugosa and Z. oenoplia, J. Bangladesh Academy of Science, 1997, 21, 151-156. (77) Contributor of IUPAC Technical Reports: Protocols on Safety, Efficacy, Standardization, and Documentation of Herbal Medicine, Pure Appl. Chem., Vol. 80, No. 10, pp. 2195-2230, 2008. Presentation in Conference/symposium/seminar/workshop (selected) 1.Mohammad Shoeb and Nilufar Nahar, Natural Products for Drug Discovery in Bangladesh,38 Annual Conference of Bangladesh Chemical Society (BCS) 31 March 2017, Chittagong, Bangladesh 2.Mohammad Shoeb, Emerging Contaminants in Marine Environment of the Bay of Bengal, 17th Asian Chemical Congress, 23-28 July 2017, Melbourne, Australia 3.Mohammad Shoeb, Dissipation of Pesticides in Vegetable Samples for Consumer Safety, 17th Asian Chemical Congress, 23-28 July 2017 Melbourne, Australia 4.Mohammad Shoeb and Nilufar Nahar, Natural Products for Drug Discovery in Bangladesh,17th Asian Chemical Congress, 23-28 July 2017 Melbourne, Australia 5.Mohammad Shoeb, ANFEC Board meeting, 26 January 2017, University of Laos, Lao PDR. Cost ANFEC 6.Mohammad Shoeb participated a series of meeting in Stockholm and Kiruna on the topic of the future of industry to discuss the importance of digital transformation, sustainable production andindustrial skills enhancement within industry, 7-13 July 201, Sweden. Cost Swedish Institute. 7.Mohammad Shoeb visited Shimadzu Laboratory GADC, Kyoto, Japan, 23-26 March 2017. Cost. AQC, Dhaka. 8.Mohammad Shoeb visited Shimadzu (Asia Pacific) Pte Ltd., and attended training course on Principles, Operation and Maintenance of LCMS-8050 Triple Quadrupole UFMS with LabSolutions Workstation. 21-22 March 2017, Singapore. Training. Cost. AQC, Dhaka. Cost. AQC, Dhaka. 9.Mohammad Shoeb participatedUnderstandingTraining Course on ISO/IEC 17025:2005(Testing & Calibration Laboratories)organized by Bangladesh Accreditation Board, Dhaka, 11-13, April 2017.

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10.Mohammad Shoeb attended 6th Outcome Mapping Review Workshop of BFSLN20 June 2017, Dhaka, Bangladesh. 11.Mohammad Shoeb attended 17th Asian Chemical Congress, 23-28 July 2017, Melbourne, Australia. 12.Professor Mohammad Shoeb; Strategy to Improve Food Safety and Quality in Bangladesh,One Day Symposium on Food Safety and Quality for Better Health, MHK Biggan Bhaban, Room No. 300 (Physics Auditorium), 07 August, 2017. 13.M. Shoeb, P. Mondal and N. Nahar, Chemical and Biological Activity Natural Occurring Mushroom. (Poster), 16th Asian Chemical Congress, 16-19 March 2016, Dhaka, Bangladesh 14. M. Shoeb, N. Nahar, Drug Discovery from Natural Products. (Invited Lecture), 1st Symposium on Chemistry for Global Solidarity, 14 October, 2016, Department of Chemistry, Jagannath University, Dhaka, Bangladesh 15. M. Shoeb, N. Nahar, Natural Products for the Sustainable development of health care in Bangladesh. (Invited Lecture), Natural Products to Combat Problems of the Developing World, 19-20 December 2016, Manchester, UK 16. M. Shoeb, N. Nahar, Bioactive secondary metabolites from antidiabetic plant materials and endophytic fungi (Invited Lecture), 8th Asian Network of Research on Antidiabetic Plants (ANRAP), 23-25 November 2015, Malaysia 17. M. Shoeb, P. Mondal, N. Nahar, Chemical and biological activity studies of naturally occurring mushroom (Invited Lecture), 2nd International Bose Conference-2015, 3-4 December 2015, Dhaka, Bangladesh 18. M. Shoeb, N. Nahar, Fungal endophytes as potential sources of bioactive natural products (Oral), Microbiology International Conference-2015 & 29th Annual General Meeting, 26-28 December 2015, Dhaka, Bangladesh 19. Mohammad Shoeb, Endophytic fungus are sources of novel pharmaceuticals (Poster), 19th Conference of Islamic World Academy of Sciences (IWAS), Dhaka, 5-9 May, 2013 20. Mohammad Shoeb, Natural Products from Endophytic Fungi (Oral), Bangladesh Sciences, Challenges of 21 Centuries, Asiatic Society, Dhaka, Bangladesh, 02 November 2013 21. Mohammad Shoeb, Isolation and structure elucidation of bioactive compounds from endophytic fungi (Oral), 3rd NRCT-IFS Workshop, Bangkok, Thailand, 28 Nov-04 Dec, 2013 22. Mohammad Shoeb, Bioactive compounds from endophytic fungi of Bangladesh (Invited Lecture), Asian Symposium on Medicinal Plants, Species and Other Natural Products (ASOMPS XVI), Karachi, Pakistan 09-13, December 2013

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23. Shoeb, M., Hoque, E., Ebel, R., Jaspars, M. and Nahar, N., Secalonic acid derivatives from endophytic fungi isolated Ocimum basilicum, a medicinal plant of Bangladesh, 41st Scottish Regional Meeting of the RSC Organic Division, University of St Andrews, 12th December 2012 (abstract published only) 24. Shoeb, M. Studies of endophytic fungi from medicinal plants of Bangladesh, 12th Eurasia Conference on Chemical Sciences, 15-21 April 2012, Corfu, Greece (Oral). 25. Mohammad Shoeb, Herbal Medicine for the Sustainable Development of Health Care (Invited Lecture), The First International Convention of Society of Pharmacognosy, Belgaum, India, February 18-20, 2011. 26. Mohammad Shoeb, Phytochemical and bioactivity studies of some medicinal plants of Bangladesh (Poster), 42nd IUPAC Congress: Chemistry Solutions, Glasgow, UK, 02-07 August 2009. 27. Shoeb, M., Natural products from endophytic fungi, 6th International on Antidiabetic Plant Materials on Separation Techniques and Biological Testing (Oral), Dhaka, Bangladesh, 15-17 January 2010. 28. Shoeb, M., Begum, S., Nahar, N., Bioactive Compounds from Endophytic Fungi (Oral), Bangladesh Chemical Congress 2008, Dhaka, Bangladesh, 31 Janaury-03 February 2009 29. Mohammad Shoeb, Chemical Contaminants in Food stuff of Bangladesh, 38th International Symposium on Environmental Analytical Chemistry-ISEAC 38, June 17-20, 2014, Lausanne, Switzerland. 30.Mohammad Shoeb, Food Safety and Challanges for Developing Country, University of Dar es Salaam, Dar es Salaam, Tanzania, 16 November 2013. 31. Mohammad Shoeb, Chemical contaminants in food and human blood samples of Bangladesh, ICBBCS, Online presentation, Virtual Education Project Pakistan, ICCBS, Karachi, Pakistan, 01 November 2013. 32. Mohammad Shoeb, Chemical contaminants in agricultural food stuff of Bangladesh, 12th International & 24th National Chemistry Conference, Bahauddin Zakariya University, Multan, Pakistan, October 28-30, 2013. 33. Mohammad Shoeb. Chemical safety and security managements for better life, Seminar on the Chemical Weapons Convention and Chemical Safety Management for Member States of the OPCW in the Asian Region, Doha, Qatar 11-12 December 2012 (Oral). 34. Mohammad Shoeb. Regional collaboration for chemical safety managements, Workshop on article XI of the chemical weapons convention for the Asia Regions, Beijing, China, 3-5 September 2012 (Oral).

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35. Mohammad Shoeb, Halogenated organic pollutants in food and human blood samples of Bangladesh (Invited speaker), Trends and Application of Biocontrol and Monitoring Techniques for Sustainable Agriculture, Korea, October 04, 2011. 36. Mohammad Shoeb, Environmental contaminants in some stuffs in Bangladesh, The 2nd Workshop on Organic Pollutant in Environment, National University of Laos, Vientiane, Laos, 28 December 2010. 37. Mohammad Shoeb, Organic Pollutants in food, human blood and environment of Bangladesh, Pacifichem Congress, Honolulu, Hawaii, USA, December 15-20, 2010. 38. Mohammad Shoeb, International Cooperation and exchange, OPCW Workshop on Article X1, The Hague, The Netherlands 24-25 November 2010. 39. Mohammad Shoeb, Shoeb, Dissipation of pesticide in tomato, tea and rice in Bangladesh, 3rd EuCheMS Chemistry Congress, Nurnberg, Germany, 29 August-02 September 2010. 40. Mohammad Shoeb, Environmental Contaminants in Food of Bangladesh, Invited Seminar at the Department of Pharmacy, University of Wolverhampton, UK 10 August 2009. 41. Mohammad Shoeb, Screening of Environmental Contaminants in Food of Bangladesh, Invited Seminar at the Department of Chemistry, University of Aberdeen, UK, 29 July 2009. 42. Mohammad Shoeb, Investigation of nitrofuran antibiotics and organochlorine pesticides in shrimp cultivation in Bangladesh, EuCheMS International Conference on Chemistry and the Environment, Stockholm, Sweden 14-17 June 2009. 43. Mohammad Shoeb, Organic pollutants in food and environmental samples of Bangladesh, EuCheMS International Conference on Chemistry and the Environment, Stockholm, Sweden 14-17 June 2009. 44. Mohammad Shoeb, Investigation of Organic Pollutants in Food and Environmental Samples in Bangladesh, Departmental Seminar at the Department of Environmental Chemistry, University of Stockholm, 18 November 2008. 45. Mohammad Shoeb, Phytochemical and antidiabetic activity studies of Crataeva religiosa Hook leaves, 41st IUPAC Congress, Turino, Italy, 05-11 August 2007. 46. Mohammad Shoeb, Investigation of Hazardous Chemicals Used in Shrimp Cultivation, Saskatoon International Validation Workshop for Regulatory Analysis of Residues in Foods, Saskatoon, Canada, June 10-14, 2007. 47. Mohammad Shoeb, MacManus, S., Jaspars, M., Sarker; S., Bioactive Compounds from the Seeds of Centaurea montana, The Fifth International ANRAP (International Network on Antidiabetic Plants) Seminar, Kuala Lumpur, Malaysia, 08-10 November 2006.

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48. Mohammad Shoeb, Phytochemical and Biological Activity Studies on Centaurea Schiskinii Seeds, The International Conference on Saponin, Phytochemistry and Application of Plant Saponins, Pulawy, Poland, 08-10 September, 2004. 49. Mohammad Shoeb, Phytochemcial and Biological Activity Studies on three Endemic Turkish Species of the Genus Centaurea, Future Trends in Phytochemistry: A Young Scientists Symposium, Garda Lake, Italy, 04-06 May, 2004. 50. Thirty Second Scottish Regional Meeting, RSC, University of Edinburgh, UK, 17 December 2003. 51. Thirty First Scottish Regional Meeting, RSC, University of Dundee, UK, 18 December 2002. 52. Mohammad Shoeb, Third IUPAC International Conference on Biodiversity, Antalya, Turkey, 3-8 November 2001.

Student Supervisions (Joint)

Ph.D. awarded: 6 M.Phil. Awarded: 3

Current Ph.D: 3 M.Phil. 1

MS students: 77 (Joint supervision) BS (4th Year project): 63 (Joint supervision)

PhD degree awarded

Gender Name Year Title Female Abida Sultana 2017 Chemical Contaminants in Rice, Spice and Vegetable Samples Female Zerin Sultana

Munia 2017 Determination of the Toxicity Level of Imidacloprid in Rice

Sample Produced in Bangladesh Male Md. Nasir

Uddin Al Mahmud

2014 Studies of Dissipation Pattern and Residue Analysis of Pesticides in Paddy Cultivation System, Vegetables and Soil

Male Rausan Zamir 2009 Studies on halogenated organic pollutants in human blood and biota in the environment, Department of Chemistry

Female Farzana Khalil 2017 (submitted)

Dichlorodiphenyltrichloroethane in Environmental Samples and Human Blood of Chittagong Chemical Complex Area and Pesticide Residues in Some Vegetable Samples

Male A. B. M. Mahfuzul Alam

Due in 2017

Method development for quantification of some non-pharmacopeial pharmaceutical drugs

M.Phil. degree awarded

Female Shahanara Begum 2009 Studies of Secondary Metabolites from endophytic fungi of Terminalia chebula Retz and Aquilaria malaccensis Lamk

Male Hiron Moy Sarma 2011 Studies of antibiotics in fish, poultry and poultry feeds and contaminants in food stuffs

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Male Md. Mozammel Haque 2013 Isolation and structure elucidation of biologically active secondary metabolites from endophytic fungi

Current Ph.D/M.Phil students

Male Md. Shahed Reza; [email protected]

PhD Studies of contaminants (multi pesticide residues) in fruits and vegetable

Female Rafiza Islam; [email protected]

Ph.D Investigation of Antibiotic Residues in Meat, Milk and Egg Samples

Male Md. Mizanur Rahman; [email protected]

Ph.D Isolation and Structure Elucidation of Secondary Metabolites from Three Medicinal Plants

Male Farhana Sobnom; [email protected]

M.Phil. Level of organophosphorus insecticides in the diet and blood samples of an adult rural population in relation to their cardiovascular risk

Student Supervision (Joint supervision)

PhD degree awarded: 06

Gender

Name Year Title

Female

Abida Sultana

2017 Chemical Contaminants in Rice, Spice and Vegetable Samples

Female

Zerin Sultana Munia

2017 Determination of the Toxicity Level of Imidacloprid in Rice Sample Produced in Bangladesh

Male Md. Nasir Uddin Al Mahmud

2014 Studies of Dissipation Pattern and Residue Analysis of Pesticides in Paddy Cultivation System, Vegetables and Soil

Male Rausan Zamir

2009 Studies on halogenated organic pollutants in human blood and biota in the environment, Department of Chemistry

Female

Farzana Khalil

2017 (submitted)

Dichlorodiphenyltrichloroethane in Environmental Samples and Human Blood of Chittagong Chemical Complex Area and Pesticide Residues in Some Vegetable Samples

Male A. B. M. Mahfuzul Alam

Due in 2017

Method development for quantification of some non-pharmacopeial pharmaceutical drugs

M.Phil. degree awarded: 03

Female

Shahanara Begum 2009 Studies of Secondary Metabolites from endophytic fungi of Terminalia chebula Retz and Aquilaria malaccensis Lamk

Male Hiron Moy Sarma 2011 Studies of antibiotics in fish, poultry and poultry feeds and contaminants in food stuffs

Male Md. Mozammel Haque

2013 Isolation and structure elucidation of biologically active secondary metabolites from endophytic fungi

17

Current Ph.D/M.Phil students: 04

Male Md. Shahed Reza; [email protected]

PhD Studies of contaminants (multi pesticide residues) in fruits and vegetable

Female Rafisa Islam; [email protected]

Ph.D. Investigation of Commonly Used Antibiotics in Meat, Egg and Milk Samples

Male Md. Mizanur Rahman; [email protected]

Ph.D. Isolation and Structure Elucidation of Secondary Metabolites from Three Medicinal Plants

Male Farhana Sobnom; [email protected]

M.Phil. Level of organophosphorus insecticides in the diet and blood samples of an adult rural population in relation to their cardiovascular risk

MS degree awarded

Gender Full Name & e-mail Address Year Title of thesis Male Md. Shakhawat Hossian 2006 Chemical and biological Studies of Momardica

charantia and Irrivingia gabonensis Male Md. Farouq Ahmed 2006 Isolation of secondary metabolites from Herbal medicine

(Ziabetes) Male Md. Golam Kibria 2006 Isolation of secondary metabolites from Endophytic

fungi of Memordica charantia Male Ariful Islam 2007 Analysis of Organochlorine pesticide residues in some

fish, Dry fish, vegetables and fruit samples Male S.M Azizul Haque 2007 Analysis of Organochlorine pesticide residues in some

fish and dry fish samples Male Mosharof Hossain 2007 Analysis of Organochlorine pesticide residues in Rui,

Katla and Pangus fish samples Female Sharmin Nishat 2007 Isolation of secondary metabolites from Myristica

fragrans seeds Female Naznin Nahar 2007 Evaluation of two antidiabetic herbal medicines-

Jambadayrist and handmade bori Male Sankar Mandal 2008 Investigation of Antibiotics and Organochlorine

Pesticides in Poultry Feed, Feed Ingredients, Poultry Meat and water Samples

Female Sabyasachy Mistry 2008 Isolation of Neolignans and phenolic acids from the seeds of Myristica fragrance (Nutmeg)

Male Nirob Kumar Saha 2008 Chemical and biological studies on ‘Murraya koenigii’ (Curry leaves).

Male Mohammad Saiful Islam Seraji

2008 Investigation of Antibiotics and Organochlorine Pesticides in Fish, Fish Feed, Feed Ingredients and Water samples

Male Mahbubur Rahman 2008 Isolation and structure elucidation of secondary metabolites from Endophytic Fungi of Leucus india and Agar Agar

Male Rabiul Hoque Bin Kashem 2008 Investigation of DDTs (DDT, DDD & DDE) in soil and sediments from Chittagong Chemical Complex.

Male Mushfiqur Rahman 2009 Analysis of organohalogen compounds in human blood samples

Male Mohammad Rezaul Karim 2009 Analysis of organochlorine pesticide residue in poultry meat collected from Dhaka

Male Ahmedul Kabir 2009 Study of Dissipation of Cypermenthin in Tomato Samples

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Female Afsana Alam Mahim 2009 Analysis of organochlorione pesticide residue in poultry meat collected from Joydevpur

Male Mir Mahadi Hassan [email protected]

2010 Study of the Purity of formulated Cypermethrin and its Dissipation pattern in Tomato

Female Rifat Farhana [email protected]

2010 Analysis of residual organohalogeneted compounds (DDT and its metabolites) in different parts of Mola fish sample

Male Abdullah-Al- Mamun [email protected]

2010 Analysis of organohalogeneted compounds and its metabolites in small fish sample

Male Mahfuzur Rahman Jony [email protected]

2010 Isolation of secondary metabolites from Penicilium thiommi, an endophytic fungus from Terminalia Chebula Retz

Male Kazi Kaium Hossain [email protected]

2010 Analysis of essential oils of local plants by HPLC and Gas Chromatography

Female Sipra Rani Shutra Dhar 2011 Studies of dissipation pattern of chlorpyrifos in water sample collected from experimental rice field of BRRI

Female Zerin Sultana Munia 2011 Studies of bioaccumulation of organohalogen compounds (DDTs) in Kachki (Corica soborna) Fish

Male Jeeshan Ahmed 2011 Development of clean up method for diazinon from soil Matrix

Male Hiron Moy Sarma 2011 Studies of antibiotics in fish, poultry and poultry feeds and contaminants in Food Stuffs

Male Mahmuda Fardous 2011 Analysis of DDTs in different parts of Mola fish sample Male Rayhan Hossain Rubel 2011 Development of clean up method for chlorpyrifos from

soil Matrix Male Saifur Rahman Azad 2011 Analysis of heavy metals in soil and rice seedings (Cd,

Cr and As) Male Md. Shahidul Islam Khan 2011 Analysis of heavy metals (Pd, Ni and As) in soil and rice

seedings Male Mohammad Suman Haque 2011 A study of the quality control parameters in some orange

juice- drink of Bangladesh Male Md. Mafizul Islam 2011 Determination of purity of turmeric in powder available

in local market of Bangladesh Male Md. Robiul Islam 2012 Analysis of DDTs in soil and water samples Female Tahmina Afroz 2012 Analysis of residual DDTs in different parts of Katla fish

sample of Chalanbeel area and fatty acid composition of the fish oil

Female Farzana Hossain 2012 Analysis of organochlorine pesticide (DDTs) residues and fatty acid compositions in different parts of rui fish samples from Chalanbeel area

Female Farzana Yasmin 2012 Analysis of residual DDTs in different parts of Boal fish sample of Chalanbeel area and the fatty acid composition of the fish oil

Male Kamal Kanti Roy 2012 Determination of fatty acid compositions of some cooking oil in Bangladesh

Female Rahela Akhter 2012 Study of Polar Secondary 1-Butanol Extract of Corypha taliera Roxb

Female Tamanna Azam 2012 Study of secondary metabolites from dichloromethane (DCM) extract of Corypha taliera Roxb

Male Sultan Ahmed 2012 Study of Fatty acid composition of Mola Fish (Amblypharyngodon mola) samples

Female Nabera Nahrin 2012 Determination of residual amount of diazinon in rice straw and rice seedling samples

19

Female Siffat –E Nusrat 2012 Chemical investigation of Corypha taliera Roxb Male Md. Ripon Hossain 2012 Study of fatty acid composition and of heavy metals in

some rice samples of Sonargoan area Male Lenin Sourav 2012 Method development and validation for the analysis of

fenvalerate in water by HPLC Male Muhammad Shamim Al

Mamun; [email protected]

2013 Analysis of residual amount of DDTs in Rupchanda fish (pomfret) and fatty acid composition of fish oil

Male Radwan Ebna Noor; [email protected]

2013 Study of residual amount of DDTs in Koral (Lates calcarifer) fish and it’s fatty acid composition

Male Abdullah Al Imran; [email protected]

2013 Analysis of residual amount of DDTs in different parts of Ruhi and Catla fish samples from Chalan Beel area and fatty acid composition of the fish oil

Male Md. Tushan Shahdat 2013 Analysis of Free Sugar and Dietary Fiber of two different varieties of mango (Himsagar and Ashwinibhog)

Male Md. Shamim; [email protected]

2013 Analysis of Free Sugar and Dietary Fiber of two different varieties of mango (Langra and Amrupali)

Male Hanifur Rahman; [email protected]

2013 Studies of fatty acids composition change in mustard oil heated with (vanillic and protocatechuic acid)

Male Topu Kumar Bhoumik; [email protected]

2013 Studies of fatty acids composition change in soya bean oil heated with protocatechuic and vanilic acid

Female Niamat-e-Khuda 2014 Study of pesticide residues in Bitter gourd (Momordica charantia)

Male Mohammad Moniruzzaman 2014 Study of pesticide residues in Momordica dioica (Kakrol) collected from different area of Bangaldesh

Male Md. Salauddin Kader 2014 Study of chemical composition of chickpea (Cicer arietinum L.)

Male Abdullah Al Anid 2014 Study of chemical composition of fieldpea (Pisum sativum)

Female Lotefa Binta Tuli 2014 Identification and quantification of pesticide in packet rice samples of Pran and Aarong brands

Female Waziha Farah 2014 Investigation and determination of pesticide residues in Abelmoschus esculentus (LadiesFinger) collected from different areas of Bangladesh

Male Waliul Islam Khan 2014 Determination of composition of camel milk Female Leudmila Haque 2014 Analysis of residual amount of DDTs in fish samples

and fatty acid composition of fish oil Male Shaurav Sarker 2015 Studies of polyaromatic hydrocarbons in downstream

water samples from Buriganga, Meghna, Turag rivers and Hatirjheel lake.

Male Md. Sharear Saon 2015 Evaluation of locally produced Vitamin B1, B2, and B6 by UV-VIS spectrophotometric.

Male Prithu Mondal 2015 Chemical and Biological Activity Studies of Mushrooms.

Male Md. Nazimuddin 2015 Studies of Polyaromatic Hydrocarbons in Upstream Water Samples from Buriganga, Meghna, Turag rivers and Gulshan Lake.

Male Md. Ahasan Ul Hoque Sakib 2016 Investigation of Three Surfactants in Water by Spectrophotometric Method.

Male Md. Golam Kibria 2016 Fatty acid compositions and bioaccumulation of heavy metals in fishes of different trophic level.

20

Male Md. Amjad Hossain 2016 Organochlorine Pesticides and Fatty Acid Composition in Fresh water Fish samples.

Female Nasrin Jahan 2016 Nutritional and Phytochemical Study of Some Edible Vegetables.

Female Sharmin Sultana 2016 Assessments of chemical constituent in some pesticide treated and untreated vegetables.

Male Rabiul Islam 2017 Studies of fatty acid compositions and level of Cr and Hg in marine fish samples.

Male Md. Mazharul Islam 2017 Organochlorine Compounds and Arsenic, Cadmium and Lead in Marine Fish Samples of Bangladesh.

Male Anowar Hossain Khan 2017 Organochlorine Compounds and Heavy Metals in Sediment Samples from Coastal Area of Bangladesh.

Female Jannatun Noor Jeouty 2017 Studies of microplastic, organochlorine compounds and toxic metals in marine fish samples of Sundarban region.

Female Rehnuma Tabassum 2017 Study of Water Quality Parameters and Environmental Contaminants in the Aquatic Ecosystem of the Bay of Bengal.

Female Most. Salma Khatun 2017 Bioactive Secondary Metabolites from Plant Averrho abilimbi L.

BS (4TH YEAR RESEARCH PROJECT)

Gender Full Name & e-‐mail Address Year Title of thesis

Male Md. Mahbubur Rahman 2006 Secondary metabolites from Entophytic fungus

Male Nirob Kumar Saha 2006 Chemical and biological Studies of Iviringa gabomensis

Male Md. Anamul Haque 2006 Chemical and biological Studies of root bark of Oroxylam indicum, a traditional medicinal plant of Bangladesh

Male Mohammad Saiful Islam Seraji

2006 Analysis of fatty acid composition of some fish samples

Male Sankar Mandol 2006 Identification and quantification of ripening agents (ethephon) in tomatoes

Male Sabyasachy Mistry 2006 Analysis of PCBs in three different river water samples of Bangladesh

Male Mohammad Sharifur Rahman

2007 Analysis of Organochlorine pesticide residues in some dry and fresh fishes

Male Md. Omar Faruk 2007 Organochlorine pesticide residues analysis in some dry and fresh fishes

Male Khorshada Jahan 2007 Isolation of bioactive compounds from endophytic fungus of vinca rosea

Male Md. Monarul Islam 2007 Isolation of secondary metabolites from Herbal medicine Dolabi

Male Mosharruf Hossain 2008 Chemical and biological Studies of the DCM extracts of the leaves of Murraya koeinigii

Male Md. Alauddin 2008 Isolation of compounds Barun Pata

Female Shaila Alam 2008 Analysis of water extract of Lin seed

21

Male Md. Rezaul Karim 2008 Analysis of DDTs in water samples of Barabkundu (DDT factory area)

Male Masud Prvez 2008 Analysis of Organochlorine pesticide in poultry Feeds

Male Habibur Rahman 2008 Analysis of Organochlorine pesticide in poultry Feeds

Female Subrina Sultana 2009 Studies of bioaccumulation of organochlorine compounds in the meat of broiler chicken purchased from Hatir Pool Bazar of Dhaka City

Female Shipra Rani Shutra Dhar 2009 Studies of bioaccumulation of organochlorine compounds in the meat of broiler chicken purchased from Dhaka New Market Kacha Bazar of Dhaka City

Female Zerin Sultana Munia 2009 Studies of bioaccumulation of organochlorine compounds in the meat of broiler chicken purchased from Kaptan Bazar of Dhaka City

Female Farzana Yasmin 2010 Determination of purity cypermethrin in commercial grade samples

Female Leudmila Haque 2010 Determination of DDT in Poultry Feed

Male D. H. Asraful Habib 2010 Determination of DDTs in Poultry Feed

Male Samiul Azam 2010 Determination of fatty acid composition in poultry feed

Female Farzana Hussain 2010 Determination of DDTs in Poultry Feed

Male Robiul Islam 2010 Determination of purity of fenvelerate in commercial grade samples

Male Sultan Ahmed 2010 Organo Chlorine Pesticide and its Residue in Poultry Feed Samples

Female Nabera Nahrin 2010 Determination of residual pesticide in water samples collected from the rice fields after the first application of double of the recommended dose of chlorpyrifos

Female Shefat –E Nusrat 2010 Determination of residual pesticide in water samples collected from the rice fields after the first application of double of the recommended dose of diazinon

Male Md. Ripon Hossain 2010 Determination of residual pesticide in water samples collected from the rice fields after the first application of double of the recommended dose of diazinon

Male Lenin Sourav 2010 Recovery of diazinon in water

Male Md. Korban Ali 2010 Determination of residual pesticide in water samples collected from the rice fields after the first application of double of the recommended dose of chlorpyrifos

Male Md. Ariful Hoque 2010 Recovery of diazinon in water

22

Male Saimul Azam 2010 Studies of fatty acid composition in some poultry feed samples

Male Muhammad Shamim Al Mamun; [email protected]

2011 Recovery of chloropyrifos in water samples (analysis by GC-‐ECD)

Male Radwan Ebna Noor; [email protected]

2011 Recovery of diazinon in water samples (analysis by GC-‐ECD)

Male Abdullah Al Imran; [email protected]

2011 Recovery of chloropyrifos in water samples (analysis by LC-‐PDA)

Male Md. Hanifur Rahman; [email protected]

2011 Recovery of diazinon in water samples (analysis by LC-‐PDA)

Male Topu Kumar Bhoumik; [email protected]

2011 Recovery of chloropyrifos and diazinon in water samples (analysis by GC-‐ECD)

Female Lotefa Binte Tuli 2012 Determination of DDTs and fatty acid composition in boal fish sample

Female Farhana Sharmin 2012 Determination of percent fat and its composition of Lexus biscuit (Imported)

Male Mohammad moniruzzaman 2012 Method development for analysis of polyaromatic hydrocarbon (phenanthrene) in water matrix by GC-‐FID

Male Mohammad Rasel Mian 2012 Method development for analysis of polyaromatic hydrocarbon (Naphthalene) in water matrix by GC-‐FID

Female Niamat-‐E-‐Khuda 2012 Method development for analysis of polyaromatic hydrocarbon (Anthracene) in water matrix by GC-‐FID

Male Md. Salauddin Kader 2012 Determination of percent fat and its composition of Tips biscuit (locally made)

Male Abdullah Al Anid 2012 Determination of percent fat and its composition of Energy Plus biscuit (locally made)

Female Mst. Nazmin Nahar; [email protected]

2013 Studies of Polycyclic Aromatic Hydrocarbons (PAHs) in Tap water samples in Dhaka City Area

Male Anam Chandra Paul; [email protected]

2013 Studies of Polycyclic Aromatic Hydrocarbons (PAHs) in lake water samples of Dhaka city area

Male Md. Nazimuddin; [email protected]

2013 Studies of Polycyclic Aromatic Hydrocarbons (PAHs) in pond water samples of Dhaka city area

Male Prithu Mondal; [email protected]

2013 Analysis of fatty acid in ‘Tip’ Biscuit samples of Dhaka city area

Female Tahni Jahrina; [email protected]

2013 Determination of fatty acid contents in Biscuit samples of Dhaka city area

Female Nasifa Akter; [email protected]

2013 Determination of total fat content & fatty acid composition in Energy Plus biscuit samples of Dhaka city area

23

Male Md. Sakib Ahasanul Hoque 2014 Determination of metronidazole in pharmaceutical dosage forms

Male Md. Golam Kibria 2014 Estimation of paracetamol by Spectrophotometric analysis

Male Robiul Islam 2015 Qualitative Estimation of Preservative and Stimulant in soft drink 7UP by UV-‐Visible spectrophotometer.

Male Md. Mazharul Islam 2015 UV-‐Visible Spectrometric studies for Assessment of Sodium Benzoate and Caffeine in Sprite Sample.

Male Anowar Hossain Khan 2015 Evaluation of Diclofenac Sodium in Pharmaceutical Preparations by UV-‐Visible Spectrophotometer.

Female Rehnuma Tabassum 2015 Estimation of Anionic Surfactant Sodium Dodecyl Sulphate in water from Ramna Lake, Hatirjheel Lake and Dharla River of Dhaka city using Spectrophotometric Technique.

female Salma Akter Mou 2016 Studies of Food Preservatives and Color additives in orange Jelly Samples by UV –visible spectrophotometer.

Female Tanzina Akther 2016 Study of physiochemical properties and Analysis of sodium benzoate, caffeine in jam samples.

Male Mafizul Islam 2016 Quantitative estimation of methyl paraben in chilli sauce by UV-‐vis spectrophotometer.

Male Saiful Islam Bhuyian 2016 Studies of physico-‐chemical properties of orange soft drinks and determination of food additives by UV-‐visible spectrophotometric method.

Male Tofael Ahmed 2016 Studies of physiochemical properties of orange juice and determination of yellow 6 by UV-‐visible spectrophotometric method.

Male Saiful Islam 2016 Determination of methyl paraben and lycopene in tomato ketchup by UV-‐Visible spectrometry method

1

Enclosure 6

Theses /Abstracts

1. Abida Sultana, PhD (Graduation: 2017) Chemical Contaminants in Rice, Spice and Vegetable Samples

Abstract The amount of food produced is very important as the human population increases. Over the last 40 years, food production has been increased 20-‐50 % where pesticides were played an important role. Proper use of pesticides can protect storage food and vegetable from damage without causing any obvious toxic effects, and final residues of pesticides in edible parts are under recommended maximum residue levels (MRLs). In developing countries, including Bangladesh, the cultivation of crops is mainly carried out by small farmers. To get more products, they use pesticides in overdose than needed in many cases. These are creating serious health problems in Bangladesh. As a part of PhD work, some survey about the present pesticides used in field and storage level have done. A total of 94 pesticides, with 299 trade names, of different groups and formulations, have been registered for use in agriculture. From the observation of the most recent government figures available, the total pesticides imported in Bangladesh are increases gradually. However, our field survey revealed that a large number of unregistered pesticides are being used for storage food and vegetable samples in the country. Therefore, the present research project has been undertaken to determine the residual pesticides/natural toxins in stored food and vegetable samples. In all the analyses certified standard reference samples (91-‐99 % purity) were used. Rice is cultivated in three seasons in Bangladesh. As Bangladesh is a hot and humid country moisture content is increased even the crops dried properly. For this climate of Bangladesh, aflatoxins (natural mycotoxins that are produced by certain molds) can be grown in rice. The aim of the study was to assess the level of aflatoxins (if any) in some rice samples. Rice samples were collected from three districts of Bangladesh (Dhaka, Noakhali and Kurigram). The samples were extracted with aqueous methanol and the extract was purified by immunoaffinity column. The analytes were identified and quantified by reverse-‐phase high performance liquid chromatography where KOBRA Cell was attached after column for post-‐column bromo derivatisation (PCD) which gave fluorescence. Calibration curves were linear with coefficient of determinant r2 ≥ 0.9998, 09997, 0.9956 and 0.9969 for B1, B2, G1 and G2, respectively. The limit of detection (LOD) and quantification (LOQ) were 0.009 and 0.025 µg kg-‐1 for B1, 0.006 and 0.018 µg kg-‐1 for B2, 0.039 and 0.116 µg kg-‐1 for G1 and 0.025 and 0.075 µg kg-‐1 for G2, respectively. The total aflatoxins (B1, B2, G1 and G2) in the rice samples were found to be in the range of trace to 3.54 µg kg-‐1. Aflatoxin B1, B2, G1 and G2 were present in 70, 60, 40 and 10 % of rice samples, respectively. The results revealed that 18 out of 20 samples contained detectable amount of aflatoxins. Aflatoxin B1 (in the range of 0.04 to 0.70 µg kg-‐1), B2 (in the range of trace to 0.20 µg kg-‐1), G1 (in the range of 0.22 to 1.82 µg kg-‐1) and G2 (in the range of 0.12 to 1.56 µg kg-‐1) were quantified in 17, 16, 6 and 4 samples, respectively. Recoveries (n = 4) were carried out at two different spiking concentrations (1.39 and 2.77 µg kg-‐1 for B1, 0.49 and 0.98 µg kg-‐1 for B2, 1.56 and 3.12 µg kg-‐1 for G1 and 0.51 and 1.01 µg kg-‐1 for G2) and were ranged from 56.71 ± 1.60 to 70.37 ± 5.59 % for B1, 57.71 ± 0.58 to 75.36 ± 6.77 % for B2, 65.53 ± 0.73 to 72.85 ± 5.93 % for G1 and 65.83 ± 2.92 to 99.20 ± 3.16 % for G2, respectively.

Commercial grade turmeric powder samples were analyzed for the presence of carbofuran residues by high performance liquid chromatography (HPLC) coupled with photodiode array (PDA) detector. A total 46 turmeric powder samples (37 were packet and 9 were loose samples) were extracted with ethyl acetate by following QuEChERS (quick, easy, cheap, effective, rugged and safe) method. The extract was cleaned up using an open column packed with mixture of florisil, alumina and charcoal

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(5:5:1 ratio). Calibration curves were linear with coefficient of determinant r2 ≥ 0.9996, 09973 and 0.9958. The limit of detection (LOD) and quantification (LOQ) were 0.01 and 0.03 mg kg-‐1 carbofuran, respectively. No residue was found when the sample was heated in a water bath for 30 min. The amount of carbofuran residues were found to be in the range of 2.5 ± 0.07 to 23.1 ± 0.30 and 2.06 ± 0.14 to 7.8 ± 0.32 mg kg-‐-‐1 in the packet and loose samples, respectively. Recoveries (n = 7) were carried out at three different spiking concentrations (0.4, 0.8, 1.0 and 20 mg kg-‐1) and were ranged from 92.52 ± 0.01 to 103.14 ± 2.41 %. For the study of post-‐harvest intervals of diazinon and carbosulfan in cauliflower, bean, eggplant and tomato samples, the samples were collected from BARI (Bangladesh Agricultural Research Institute) experimental field. Three replicate treated samples of cauliflower, bean, eggplant and tomato and one control sample were collected from BARI at 0 (2 hours after spraying), 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 and 15 days after application of diazinon(2 mL L-‐1) and carbosulfan (1.5 mL L-‐1). Quantification of residue of diazinon was done on a gas chromatograph (GC) with an electron capture detector (ECD). Nitrogen was used as carrier and make up gas. Separations were performed on Non-‐polar (HP-‐5 MS) capillary column of 30 m long x 250 µm i.d. x 0.25 µm film thicknesses from Agilent, USA. A QuEChERS method was used for extraction using ethyl acetate as an extraction solvent, and cleanup was carried out using primary secondary amine. The linearity was excellent (r2 ˃ 0.9976, 0.9967, 0.9922 and 0.9905) in calibrations. The recoveries at three spiking levels were 99 to 105 % for tomato, 97 to 104 % for cauliflower, 89 to 108 % for bean and 93 to 104 % for eggplant with relative standard deviations in the range of 1.68 to10.64 %. The limit of quantification (LOQ) of this method was found to be 0.003 mg kg−1 whereas limit of detection (LOD) being 0.001 mg kg−1. The results revealed that the dissipation pattern of diazinon was followed first-‐order kinetic. The residues of diazinon in tomato, bean, cauliflower and eggplant were found to be in the range of 0.02 ± 0.01 to 1.66 ± 0.24, 0.005 ± 0.001 to 0.152 ± 0.007, 0.03 ± 0.01 to 4.02 ± 0.37, and 0.02 ± 0.01 to 1.29 ± 0.09 mg kg-‐1, respectively. The maximum residue limit (MRL) of diazinon on cauliflower, tomato, eggplant and bean has been fixed by CODEX is 0.5 mg kg-‐1. The diazinon residues declined to a level below the maximum residue limits within 3, 3 and 10 days for eggplant, tomato and cauliflower, respectively. The residue of diazinon was below the maximum residue limit even at 0 day (two hours after spraying) for bean. The estimated dissipation half-‐life (t1/2) of diazinon was found to be 2.63, 2.23, 1.12 and 0.90 days in cauliflower, tomato, bean and eggplant, respectively. The analysis of residue of carbofuran in tomato was done by using gas chromatography (GC) equipped with a flame ionization detector (FID). Nitrogen was used as carrier and makeup gas. Hydrogen and air were used for flame. Separations were performed on HP-‐5 (30 m long & 0.25 inner diameter) capillary WCOT quartz column. The tomato samples were extracted and cleaned up by QuEChERS method. The limit of detection (LOD) and limit of quantification (LOQ) were 0.1 and 0.3 mg kg-‐1, respectively. Calibration curves were linear over the calibration ranges with coefficient of determinants 0.9978 and 0.9967 for carbosulfan. The half-‐life (t1/2) of carbosulfan was found to be 5.25 days in tomato. According to Europion Union, the MRL value of carbosulfan in tomato is 0.05 mg kg-‐1. The residue of carbosulfan in tomato was found to be above the MRL value (0.05 mg kg-‐1 ) up to 14 days (9.43 ± 0.16 to 1.19 ± 0.06 mg kg-‐1).

Forty five vegetable samples namely bean, eggplant, cauliflower and tomato were purchased from different markets of Dhaka city and Noakhali and Kurigram districts. Cypermethrin, chlorpyrifos, diazinon, fenvalerate and quinalphos were detected in some of them. Quantification of residues was done on a gas chromatograph (GC) with an electron capture detector (ECD). Calibration curves were linear with coefficient of determinant r2 ≥ 0.9912, 09962, 0.9929, 0.9947 and 0.9907 for chlorpyrifos, cypermethrin, diazinon, fenvalerate and quinalphos, respectively. The LOD was found to be determined 0.50 µg L-‐1 for chlorpyrifos, 2.50 µg L-‐1 for diazinon and quinalphos and 5.0 µg L-‐1 for cypermethrin and fenvalerate, respectively. LOQ was found to be determined 1.65 µg L-‐1 for chlorpyrifos, 8.25 µg L-‐1 for diazinon and quinalphos and 16.5 µg L-‐1 for cypermethrin and fenvalerate, respectively. Out of 10 bean samples, the residue of chlorpyriphos was detected in 3

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samples (0.01 ± 0.01 mg kg-‐1; MRL 0.01 mg kg-‐1), cypermethrin was detected in 5 samples in the range of 0.05 ± 0.01 to 0.74 ± 0.09 mg kg-‐1 (MRL 0.05 mg kg-‐1) and fenvalerate was detected in 3 samples in the range of 0.39 ± 0.05 to 0.55 ± 0.04 mg kg-‐1 (MRL 1.0 mg kg-‐1). Among the 12 eggplant samples, the residue of chlorpyriphos was detected in 4 samples 0.02 ± 0.01 and 0.05 ± 0.01 mg kg-‐1 (MRL 0.5 mg kg-‐1) and cypermethrin was detected in 2 samples in the range of 0.04 ± 0.01 to 0.13 ± 0.01 mg kg-‐1 (MRL 0.2 mg kg-‐1). Out of 11 cauliflower samples, the residue of chlorpyriphos was detected in 10 samples in the range of 0.01 ± 0.01 to 0.79 ± 0.02 mg kg-‐1 (MRL 0.05 mg kg-‐1), cypermethrin was detected in 3 samples in the range of 0.09 ± 0.01 to 0.74 ± 0.16 mg kg-‐1 (MRL 1.0 mg kg-‐1) and quinalphos was detected in 4 samples in the range of 0.07 ± 0.01 to 0.49 ± 0.08 mg kg-‐1 (MRL 0.2 mg kg-‐1). Among the 12 tomato samples, the residue of chlorpyriphos was detected in 9 samples in the range of 0.01 ± 0.01 to 0.33 ± 0.02 mg kg-‐1 (MRL 0.2 mg kg-‐1) and cypermethrin was detected in 3 samples in the range of 0.05 ± 0.01 to 0.32 ± 0.04 mg kg-‐1 ( MRL 0.5 mg kg-‐1). The average recovery of chlorpyrifos in tomato (n = 5) was 98.48 ± 2.73% and in eggplant (n = 6) was 99.57 ± 6.98 % at spiking level of 0.05 mg kg-‐1. In bean (n = 3), the average recovery of chlorpyrifos was 88.51 ± 2.64 % at spiking level of 0.15 mg kg-‐1. The average recovery of cypermethrin in tomato (n = 5) was 79.65 ± 5.56 %, in eggplant (n = 6) was 86.29 ± 7.33 % and in bean (n = 3) was 97.43 ± 8.52 % at spiking level of 0.10 mg kg-‐1. The average recovery of diazinon in tomato (n = 5) was 109.92 ± 2.33 % and in eggplant (n = 6) was 101.41 ± 4.72 % at spiking level of 0.10 mg kg-‐1. In bean (n = 3), the average recovery of chlorpyrifos was 106.78 ± 3.55 % at spiking level of 0.15 mg kg-‐1. For fenvelarate, the average recovery in tomato (n = 5) was 90.88 ± 2.15 % and in eggplant (n = 6) was 84.10 ± 8.91 % at spiking level of 0.10 mg kg-‐1. In bean (n = 3), the average recovery of fenvelarate was 90.04 ± 9.29 % at spiking level of 0.15 mg kg-‐1. For quinalphos the average recovery in tomato (n = 5), eggplant (n = 6) and bean (n = 3) were 78.28 ± 4.85 , 85.15 ± 7.72 and 85.28 ± 2.32 % at the spiking level of 0.10,0.15 and 0.20 mg kg-‐1, respectively.

2. Farzana Saleh, PhD (Graduation: 2017), Health Effects of Isoflavones from Bangladeshi Soymilk in Postmenopausal Women

Abstract

Soy isoflavones, among other phytoestrogens, are used as the alternative to estrogen hormone replacement therapy in menopause. Concerns about potential side effects of hormone therapy have resulted in the increased interest for the usage of soy-‐based dietary supplements. Total isoflavones content and amount of individual isoflavone in the primary raw material are very important for their biological effects. The present study is aimed to determine total and individual isoflavone contents in the soy beans, soy milk and bioavailability of the isoflavones in human blood. Soybean seeds were collected from Jessore, the Southern agricultural area of Bangladesh. Oil of the bean powder was removed by extraction with n-‐hexane and isoflavones were isolated by extraction with ethyl acetate. The ethyl acetate extract was dried completely and re-‐dissolved in definite amount of acetonitrile (ACN) and analyzed by HPLC-‐PDA on C18 column using mobile phase, ACN-‐H2O (75:25; Flow rate: 0.5 mL/min; wavelength: 268 nm, loop size 20 µL). Genistein and daidzein were identified in the oil free bean extract with respect to retention time of certified standard genistein and daidzein, purchased from Sigma-‐Aldritch. Quantification of the isoflavones was done using external calibration curve of the two certified samples which were linear. LOD (S/N ratio; 3:1) and LOQ (S/N ratio; 10:1) were found to be 0.0045 & 0.0135 ppm and 0.25 & 0.75ppm for genistein daidzein, respectively. To achieve better resolution a new method was developed and validated by acetylation of the certified isoflavones and the isoflavones isolated from the bean extract in basic media. To determine bioavailability of the isoflavones in human blood, soy milk was prepared. Freeze-‐dried milk sample was extracted with n-‐hexane and isoflavones were isolated from the freeze-‐dried milk and analyzed following same procedure as it was done for bean powder. Genistein and diadzein in the milk were found to be 21.57 μg/100 mL and 14.56μg/100 mL, respectively and total isoflavone content of the

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milk was found to be 126.45µg/350 mL soy milk. In human blood, the amount of genistein and daidzein was found to be 0.19 μg/g and 0.43 μg/g, respectively. During advising the Bangladeshi post-‐menopausal women to take soy milk amount of isoflavones present in milk should be considered.

3. Zerin Sultana Munia, PhD (Defended to be graduated in 2017), Studies of Chemical contaminants in different food staff

Abstract

Imidacloprid, a neonicotinoid pesticide is allowed to use in food storage. A total of 30 rice samples and 15 wheat flour samples were analyzed to determine the residaul amount of imidacloprid. Among 30 rice samples, 9 (fragrant) packed samples were found to contain imidacloprid residues in the range 1.59 – 4.51 µg g-‐1which was greater than the MRL value (1.5 µg g-‐1 in rice, EPA 2010) and 9 (fragrant) unpacked samples were found to contain imidaclopridresidues in the range 0.06 – 1.10 µg g-‐1which was lower than the MRL value. The imidacloprid residue in non-‐fragrant rice samples were found below detection limit. In cooked rice samples, no trace of imidacloprid was observed. Among 15 wheat flour samples, imidacloprid residues were observed only in 8 samples, ranging from 0.03 – 0.44 µg g-‐1 which were lower than the MRL value (1.5 µg g-‐1 in wheat, EPA 2010). The toxic effect of imidacloprid in rice was evaluated against adults Sitophylus oryzae. The average mortality of adult weevils was 57% at 72 hours while spiking the control rice with a concentration level 1.25 µg g-‐1, therefore, a concentration lower than this value would be enough to control the growth of weevils during storage. Therefore, the results of residual analysis in rice indicate indiscriminate use of imidacloprid in the market samples during storage.

The dissipation pattern of quinalphos was studied in tomato, bean and cauliflower which were grown in the experimental field of Bangladesh Agricultural Research Institute (BARI). Quinalphos treated samples were collected from 0 (2 hours after application) to 15 successive days, transferred to the laboratory and stored in freezer ( at - 20 0C ). The amount of quinalphos residue in tomato, bean and cauliflower were found to be in the range of 0.05 – 6.31 ,0.05 – 3.10 and 0.06 – 6.5 µg g-‐1 and dissipated below MRL (0.20, 0.20 and 0.50 µg g-‐1in cauliflower, tomato and bean, EPA 2011) value within 6, 4 and 7 days after application, respectively.

The safe period of consumption and the dissipation pattern of five different pesticides; Vitaban 48 EC (chlorpyrifos), Double 50 EC (mix formulation of imidacloprid and cypermethrin), Nitro 505 EC (mix formulation of chlorpyrifos and cypermethrin), Asataf 75 SP (acephate) and Reeva 2.5 EC (lambda-‐cyhalothrin) in eight different vegetables (cabbage, cucumber, bottle gourd, sweet gourd, sponce gourd, green chili, cauliflower and tomato) were studied. The pesticides were applied in the agricultural field of Nuritola, Comilla, Bangladesh, collected, brought to the laboratory and analysed for successive 10 days keeping them at ambient temperature. The residual amount of chlorpyrifos, in tomato, bottle gourd, sweet gourd and green chili, went below the MRL after 5 days and in cabbage, cauliflower, sponce gourd and cucumber, after 8 days of application. The average half life of chlorpyrifos in the vegetables was found to be 1.14 days.

Pesticides of Nitro 505 EC; chlorpyrifos residues, in bottle gourd, tomato and sweet gourd, went below the MRL value after 3 days, in sponce gourd, green chili and cucumber,it was after 5 days and in cabbage and cauliflower, it was after 8 days of application whereas cypermethrin residues, in tomato, cauliflower, sweet gourd, sponce gourd, green chili and cucumber went below MRL value after 1 day, in case of cabbage and sponce gourd, it was after 3days of application. The average half-‐lives of chlorpyrifos and cyperethrin in Nitro 505 EC were found to be 0.92 and 0.58 days, respectively.

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Pesticides of Double 50 EC; imidacloprid residues went below the MRL value after 1day of application for tomato and green chili, after 3 days of application for cabbage, cauliflower, sweet gourd, bottle gourd and cucumber and after 5days of application for sponce gourdwhereas cypermethrin residues went below the MRL values after 1 day of application for tomato, cauliflower, green chili and after 3 day of application for cabbage, bottle gourd, sweet gourd and sponce gourd. The average half-‐lives were found to be 0.62 and 0.54 day for imidacloprid and cymermethrin, respectively in Double 50 EC. The initial concentration of lambda-‐cyhalothrin residue declined very sharply and went below the MRL value after 1 day of application. The residual amount of acephate went below the MRL value after 5 days of application for cabbage and cauliflower and after 3 days of application for the rest of the samples. The average half-‐life of acephate was 1.1 days.

Storage stability of the five pesticides in freezer (-‐200C) were done by fortifying the control vegetable samples with the five pesticides and the samples were then stored in a freezer (-‐20 0C) for about 30 days. The average recoveries of the pesticides were found in the range of 84% -‐ 93% which indicates that the pesticides were quiet persistent at freezing condition and not degraded during storage.

4. Tonima Mustafa, PhD (Submitted to be Graduated in 2017), Organohalogen Residues of Fishes from Different Trophic Levels

Abstract

Global contexts on human health hazards through pesticide residues become a serious focus and environmental issues today. Fishes are used extensively for environmental monitoring because they uptake contaminants directly from water and food. Generally the ability of the fish to metabolize organohalogen is moderate, thus contaminants load in fish are well reflective of the state of pollution in surrounding environments. The present study was conducted to assess the concentrations and patterns of organohalogen pesticide residues i.e. DDT and its metabolites (4,4-‐DDT, 2,4-‐ DDT, DDD and DDE) in fishes and prawn species of different trophic levels of four seasons from Sonargaon Upazila of the Meghna River. The samples were collected between the periods of 2015-‐2016. The number of species available in the seasons varied from twenty-‐two to twenty-‐four i.e. rainy season (twenty-‐four), autumn (twenty-‐two), winter (twenty-‐three) and summer (twenty-‐two). Analysis of the samples for DDTs residues were carried out using Gas Chromatograph with Electron Capture Detector (GC-‐ECD). The samples were extracted by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method and the sample extracts were cleaned-‐up by using H2SO4. Linearities (r2) were > 0.9950 for calibrations. The recoveries were 88.67% -‐ 104.89% (20 ng g-‐1), 70.10% -‐ 101.32% (10 ng g-‐1) and 71.64% -‐ 113.83% (5 ng g-‐1). The limit of detection was found 0.0625 ng g-‐1 in fish samples. The concentrations of total DDTs residue in fish and prawn tissues of rainy-‐season, autumn, winter and summer varied between 2.64 ± 0.35 ng g-‐1 to 191.14 ± 31.18 ng g-‐1, 16.42 ± 1.90 ng g-‐1 to 271.50 ± 6.17 ng g-‐1, 3.88 ± 0.60 ng g-‐1 to 141.57 ± 10.24 ng g-‐1 , 157.58 ± 1.15 ng g-‐1 to 1660.89 ± 157.9 ng g-‐1 wet weight (ww) respectively. The year round highest concentrations were observed in Bacha (Eutropiichthys vacha). However the lowest levels of total DDTs were observed in Kachki (Corica soborna) in rainy-‐season and summer, Khalisha (Trychogaster fasciata) in autumn and Goldachingri (Macrobrachium rosenbergii) in winter. Considering the average concentrations of total DDTs residue of four seasons, the twenty fish and prawn species that analysed in all seasons showed the chronology of Kachki (Corica soborna) < Chanda (Parambassis ranga) < Shing (Heteropnuestes fossilis) < Ghainna (Labeo gonius) < Rui (Labeo rohita) < Systomus sarana (Sharpunti) < Bata (Cirrhinus reba) < Jatpunti (Puntius sophore) < Goldachingri (Macrobrachium rosenbergii) < Foli (Notopterus notopterus) < Boal (Wallago attu) < Gulsha (Mystus cavasius) < Bele (Glossogobius giuris) < Tengra (Mystus vittatus) < Bajari-‐tengra (Mystus tengra) < Chewa (Pseudapocrypter elongates) < Meni (Nandus nandus) < Borobaim (Mastacembelus armatus) < Poa (Otolithoides pama) < Bacha (Eutropiichthys vacha). The other fishes that analysed in one or two seasons showed the chronology, Kaikka (Xenentodon cancila) < Khalisha (Trychogaster fasciata)

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<Gutum (Lepidocephalus guntea) < Magur (Clarius batrachus) < Tarabaim (Macrognatus aculiatus) < Shole (Channa striata) < Gojar (Channa marulius). In each season, the variation of DDT contents varied with fish species, when the concentrations in a fish varied between seasons. This might be attributed to the combine influence of the trophic position, feeding habits, lipid contents, physiological activities (metabolism rate, excretion rate and maturation stage etc.) of fishes and meteorological parameters i.e. temperature, humidity and rainfall to the accumulation of DDTs in fish tissues. Overall, the rank orders of average DDTs of different fish and prawn species of different seasons were carnivore > omnivore > herbivore and summer > autumn > rainy-‐season > winter. From the present study, it could be said that lower residues may be found in herbivores, lean and plant based omnivores and lean and zooplankton based carnivores; medium or higher residues may be found in fatty and animal based omnivores, lean and lower carnivores while much higher residues may be found in the fishes with bottom feeder carnivores, predators and fatty top carnivores. Analysis of total DDTs residues by one way ANOVA with LSD and Tukey HSD tests showed that significant differences in total DDTs between herbivore, omnivore and carnivore (p < 0.05). Highly significant differences between seasons (p < 0.001) were also observed. Pearson correlations analysis showed the positive relationships between DDE and DDD with lipid contents (p < 0.05). Besides, highly significant positive relationships between total DDTs with temperature with (p < 0.001) and negative relationship between humidity with total DDTs (p < 0.01) were observed. DDD (55.54%) was the major contributor to fish and prawn samples in rainy-‐season and followed by 4,4 -‐DDT (16.10%), DDE (14.35%) and 2,4-‐DDT(14.00%). In autumn season, the major contributor was DDE (53.11%) and followed by the DDD (31.79%), 2,4 -‐DDT(8.52%) and 4,4 -‐DDT(6.58%). In winter the major contributor was DDE (68.80%) and followed by DDD (18.23%), 2, 4 -‐DDT (7.63%) and 4,4 -‐DDT (5.33%). In summer, the major contributor was 4,4 -‐DDT(40.23%) and followed by 2,4 -‐DDT(27.59%), DDE (18.60%) and DDD (13.58%). Compositional distribution of DDTs and the ratios of (DDE+DDD)/DDTs contributing to the values indicating both recent and past use of DDT in the region. The concentrations of total DDTs in all the samples were within the permissible Maximum Residue Level (MRL) i.e. for human consumption recommended by FAO-‐WHO. But 20.83% species of rainy-‐season, 68.18% of autumn, 13.04% in winter and 100% in summer exceeded the maximum admissible limit recommended by European Union. However, 4.55% species of autumn and 36.36% of summer were above concentrations associated with reproductive toxicity in several species of fish. To assess human health risks, Health risk Indexes (HI) of fish and prawn samples were calculated. HIs < 1 in all fish and prawn samples indicating that the fishes are safe to consume but the daily consumption of the fishes together with other contamination in food may cause human health hazard. As DDT is a long persistent and bioaccumulative substance in the environment, intake of significant amount of these poisonous elements with our diet is a matter of great health concern.

5. Farzana Khalil, PhD (Submitted to be graduated in 2017), Dichlorodiphenyltrichloroethane in Environmental Samples and Human Blood of Chittagong Chemical Complex Area and Pesticide Residues in Some Vegetable Samples

Abstract

Pesticide is one of the most used components in current agricultural practices for protecting the crops from different kinds of pests. The widespread use of pesticides contaminates soil, water, air and crops. The result of mishandling or overusing of pesticides on rice, vegetables, fruits etc. are the foremost concern in many countries including Bangladesh. Organochlorine pesticides (OCPs), especially DDT was used in Bangladesh for crop manufacture and to abolish vector diseases from early sixties. The only DDT production factory within Chittagong Chemical Complex (CCC) area started in 1966 and soon supply started in the local area. However, due to long persistence in the environment, bioaccumulation, bio magnifications and accumulation to the fatty tissues of human over food chain, the consumption and manufacture of OCPs became restricted worldwide from

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nineties. The Stockholm Convention identified 12 persistent organic pollutants (POPs) and recently included 13 more, including DDT which are harmful for wildlife and human health and formulated a treaty in 2001 to stop production, usage and elimination of OCs pesticides where Bangladesh is a part of it and has been paying the fees regularly to the secretariat and actively participating in biannual conference (COP). DDT had been emitted in Bangladesh and the factory at CCC area was shut down as a signatory of Stockholm convention. Bangladesh closed down the DDT factory in 1995 without deciding what should happen to the stored DDT in the factory of the CCC area.

This study was shown to monitor the range of dichlorodiphenyltrichloroethane (DDT) and its metabolites (DDE &DDD)in environmental samples (soil,sediment, water and fish) and human blood from areas nearby a closed DDT factory in Bangladesh. Soil, sediment and water samples were collected on 13 July, 2011 from the CCC area in the southern, south western and eastern directions. Fifteen different fish (n=15) samples were collected from a pond in the factory area during June 2016. Thirty human blood (n=30) samples were randomly collected from people (men and women) living inside and near the factory on June 2014 to determine the level of exposure. DDTs (DDT and its metabolites) from soil and sediment samples were extracted using solvent extraction (SE), water samples by liquid-‐liquid partitioning, fish samples by solid dispersion method and finally human blood samples by Hovander and coworkers method with slight modification with a mixture of n-‐hexane: MTBE (1:1) followed by cleaned up using silica gel impregnated with conc. sulphuric acid (2:1 w/w, 1 g).Allsamples were analyzed by Gas Chromatograph equipped with an Electron Capture Detector (GC-‐ECD). Linearity’s expressed as coefficients (R2) were ≥0.995. The recoveries were 72–120% and 83–110%, with <15% RSD in soil and water, respectivelyat two concentration levels.The limit of quantification (LOQ) was 0.0165 mg kg-‐1 in soil and 0.132 μg L-‐1 in water. Higher amounts of DDTs were revealed in the southern (2.2–936 × 102 mg kg-‐1) or southwestern (86.3–2067 × 102 mg kg-‐1) track from the factory than in the eastern track (1.0–48.6 × 102 mg kg-‐1). An exemption was the soil sample collected 50 ft (15.24 m) east (2904 × 102 mg kg-‐1) of the factory. The range of DDTs in the water bodies (0.59–3.01 μg L-‐1) was approximately equal in all directions. The recovery for fish samples were conducted (n=3) at three concentrations (0.05, 0.1 & 0.2 mg kg-‐1). The recoveries were 70–105 %, with<16 % RSD. LOD & LOQ was found 0.063 µg kg-‐1& 0.206 µg kg-‐1 respectively in fish sample. The highest amount of DDT and its metabolites (8.9 μg kg-‐1) were found in the Shing fish. Boalfish showed small amount of DDTs. By using internal standard, the recoveries of human blood were 73−108 % (0.05 µg L-‐1) and 75−98 % (0.025 µg L-‐1) for CB-‐53. LOD & LOQ was found 0.025 µg kg-‐1 & 0.083 µg kg-‐1respectively, in blood sample. The concentration of ∑DDT was in the range (0−1686µg kg-‐1) of human blood samples. We established that DDTs might have been discarded randomly around the warehouse after the closing of the factory.

Vegetables are being consumed by the local people of Bangladesh almost every day. Pesticides are being used to protect the crops and there is no guide line about the safe harvesting period of the crops and MRL values for any pesticides in Bangladesh. Studies of dissipation pattern of pesticides in growing crops is necessary which will give a safe harvesting period as well as MRL value after final application. Dissipation pattern of cypermethrin in five different vegetables (tomato, bitter gourd, pumpkin, eggplant & green chili) were collected February 2016 from the farmer’s fields Norundi near Jamalpur district of Bangladesh. For these studies the samples were kept at ambient temperature. Twenty four vegetable samples (snake gourd, ridge gourd, wild ridge gourd & pointed gourd) were also collected from different locations of Bangladesh to analyze the presence of chloropyrifos, cypermethrin, diazinon and fenvelarate residues. All vegetable samples were extracted by QuEChERS method, cleaned-‐up by adsorption chromatography technique and analyzed by GC-‐ECD. Linearity’s (R2) ≥ 0.995 for matrix-‐matched standard, LOD and LOQ was 0.01 µg kg-‐1 and 0.033 µg kg-‐1 in cypermethrin,respectivly. The recoveries were 82−106 % (RSD ≤ 17 %) at two concentrations (0.25 & 1 mg kg-‐1) and storage stability was 83% (RSD ≤ 9 %). The MRL of cypermethrinin all vegetables were identifiedon 0 day samples (2 h after spray). The residue levels

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went down progressively with days and 74−88% dissipations was observed within 10 days. It was established that cypermethrin residues went lower the MRL value after 1 day of spraying in tomato (143 µg kg-‐1) & in eggplant (106 µg kg-‐1) and at 0 day(134 µg kg-‐1) in bitter gourd (Codex, 2013, 2009). The half-‐life of cypermethrinwascalculated. The most of the vegetable samples were not detrimental to health as all samples hadlower the MRL of cypermethrin.

The LOD and LOQ were found 0.8µg kg-‐1&2.64µg kg-‐1for diazinon, 0.002 µg kg-‐1and 0.007µg kg-‐1for chlorpyrifos, 0.01 µg kg-‐1& 0.033 µg kg-‐1for cypermethrin, and 0.002 µg kg-‐1& 0.007µg kg-‐1for fenvalerate, respectivly. The recovery experiments were conducted (n=3) at two concentration levels (0.25 and 0.5 mg kg-‐1).The average recoveries of the four pesticides in the four vegetables (73 – 115%) with RSD ≤8% Pesticide residues were detected in 40% of the market samples but all were below the MRL values.

Fluxapyroxad is a second-‐generation carboxamide fungicide that inhibits succinate dehydrogenase of mitochondrial respiratory chain. This study was carried out to assure the safety of fluxapyroxad residues in butter bar (moie) by developing a method and the dissipation pattern was observed under greenhouse conditions from two different treatments (T2 and T3). This experiment was carried out in the laboratory in Republic of Korea. The leaves which were grown in greenhouse at Naengcheon-‐ri, Masan-‐myeon, Gurye-‐gun, Jeollanam-‐do, Republic of Korea, from the last week of February until the first week of April, 2015. The method was developed and validated using high performance liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). The extraction was carried out by the QuEChERS, and then purified with silica solid phase extraction (SPE) cartridge. Correlation coefficient (R2) of matrix-‐matched standard was 0.998,LOD was 0.01 µg kg-‐1 and recoveries were 88% & 93% at both concentration 0.5 & 2.5 mg kg-‐1, respectively with RSD ≤ 10% and storage stability 95±7.04. The method was successfully applied to the experimental field samples, which were collected randomly at 0 to 14 days’ post application. In this study, fluxapyroxad was dissipated below the MRL value after 10 days at triple of recommended dose. The rate of disappearance was described to 1st order kinetics with half-‐life of 2.6 days. The initial residues after application were 11 and 20µg kg-‐1 on the zero day for T2 and T3 respectively. After 14 days the residues declined to 0.42 and 0.36 mg kg-‐1 for T2 and T3 respectively.

6. Anowar Hossain (Submitted to be graduated in 2017), Chemical and Biological Studies of Leaves and Twigs of Anogeissus rivularis and Some Hypoglycemic Herbal Formulations

Abstract

Hexane and Ethyl acetate extracts of leaves and twigs of Anogeissus rivularis and their sub-‐fractions were tested for anticancer activities on the six common human cancer cell lines; P-‐388, KB, HT-‐29, MCF-‐7, A549 and one normal human kidney cell line ASK Hek293. Sub-‐fractions, 1F-‐3, 1F-‐5, Et-‐1F-‐2, Et-‐1F-‐3, and Et-‐1F-‐4 were found to possess significant anticancer activities (ED50 <4, 3.40, 4.50, 4.60 and 6.37 µg/mL respectively) on P-‐388 cell lines. The same extracts and their sub-‐fractions were also tested for anti-‐HIV-‐1 RT. The two extracts showed moderate activity but the two sub-‐fractions, 1F-‐5 & Et-‐1F-‐7 were found to be very active (growth inhibition 97 % & 92%, respectively). From the active extracts four new compounds namely, [2-‐(3,4-‐Dimethoxy-‐phenyl)-‐3-‐methyl-‐5-‐propenyl-‐2,3-‐dihydro-‐benzofuran] (20), Dimer of 4,6-‐dihydroxy-‐2-‐(4´-‐hydroxy-‐benzyl)-‐benzofuran-‐3-‐one (21), 2-‐[(3´,4´-‐Dihydroxy-‐phenyl)-‐hydroxy-‐methyl]-‐4,6-‐dihydroxy-‐benzofuran-‐3-‐one (22), 2,5-‐Bis-‐(4-‐hydroxy-‐phenyl)-‐3,4-‐dimethyl-‐tetrahydro-‐furan-‐3-‐ol (23), together with thirteen known compounds namely, betulinic acid (3β-‐Hydroxy-‐19β-‐hydrogen-‐lup-‐20-‐(29)-‐en-‐28-‐oic acid) (3), 3β-‐hydroxy-‐20(29)-‐en-‐lupan-‐30-‐al (6), 29-‐Nor-‐20-‐oxolupeol (7), 3β,6β-‐Dihydroxylup-‐20(29)-‐ene (8), 3,3´,4´-‐tri-‐O-‐methylellagic acid (9), 3,3´-‐di-‐O-‐methylellagic acid (12), (s)-‐naringenin (4ʹ′,5,7-‐trihydroxyflavanone) (13), p-‐cumaric acid [(E)-‐3-‐(4-‐hydroxyphenyl)-‐2-‐propenoic acid] (14), 2,3-‐dihydro-‐p-‐cumaric acid [3-‐

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(4-‐hydroxyphenyl)-‐propanoic acid] (15), protocatechuic acid (3,4-‐Dihydroxybenzoic acid) (16), Stigmast-‐5-‐en-‐3-‐O-‐β-‐glucoside (17), loliolide IUPAC name (18), vanillic acid (3-‐methoxy 4-‐hydroxybenzoic acid) (19) were isolated by chromatographic techniques. The structure of this compounds were elucidated by spectroscopic methods using UV, FTIR, high resolution 1H & 13C NMR and Mass spectroscopy (HRMS-‐ESI-‐TOP) and Single Crystal X-‐ray Diffraction. A few local herbal formulations (13) were evaluated for antimicrobial and heavy metal contaminants (Cu, Cd, Cr, Mn, Pb and Zn). All of the herbal formulations showed the presence of different bacteria & fungi and their level were also slightly higher the safe level for consumption. Lead content in almost all of the samples (except two) exceeded the permissible limit according to WHO and US FDA. 7. A. B. M. Mahfuzul Alam (will submit soon to be graduated in 2017), Method development for

quantification of some non-‐pharmacopeial pharmaceutical drugs.

Abstract is not included here. Missing.

8. Shaurav Sarker, MS (Graduation: 2015); Studies of Polyaromatic Hydrocarbons in Downstream Water Samples from Buriganga, Meghna, Turag Rivers and Hatirjheel Lake

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are one of the environmental contaminants. A high performance liquid chromatography (HPLC) with Fluorescence detection (FD) method was established for the determination of PAHs in water samples of Lake and Rivers collected from different areas of Bangladesh. PAHs are ubiquitous in terrestrial, atmospheric, and particularly aquatic environments throughout the world and have been detected in lakes, ground waters, and rivers. Solid phase extraction (SPE) technique was successfully applied for PAHs extraction from water samples using HPLC grade acetonitrile solvent. The extraction of water samples were carried out by validated C-‐18 SPE cartridges. The water sample extracts were analyzed by HPLC-‐FD. Linearity was evaluated by constructing calibration curves of the standards. The linearities were excellent with correlation coefficients of r2 ≥ 0.994 for anthracene, r2 ≥ 0.997 fluoranthene and r2 ≥ 0.998 for benzo[a]pyrene. Five replicate recovery experiments were done for each of the anthracene, fluoranthene and benzo[a]pyrene. The average percent recoveries of anthracene, fluoranthene and benzo[a]pyrene were found to be 91.38%, 85.49% and 95.72%, respectively, and the relative standard deviation (RSD) (%) values of anthracene, fluoranthene and benzo[a]pyrene were found to be ≤5.27 %, ≤2.84 % and ≤17.55%, respectively. The method was successfully applied to determine the amount of targeted anthracene, fluoranthene and benzo[a]pyrene PAH compounds in Lake and River water samples of Bangladesh.

9. Md. Sharear Saon, MS (Graduation: 2015); Evaluation of locally produced Vitamin B1, B2 and B6 by UV-‐Vis spectrophotometer

Abstract

Simple, inexpensive, rapid and highly accurate methods were modified and validated to assay thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), and pyridoxine hydrochloride (vitamin B6), in pharmaceutical dosages. Thiamine hydrochloride of 100 mg dose of six different companies, riboflavin of 5 mg dose of five different companies and pyridoxine hydrochloride 20 and 25 mg dosages of two different companies of Bangladesh were extracted from the tablets, cleaned up and their active ingredients were evaluated by UV-‐Vis spectrophotometer at 432 445 and 292 nm,

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respectively. Evaluation was carried out with respect to calibration curves of their standard reference samples. Amount of thiamine hydrochloride in 100 mg tablets of six different companies were found to be in the range of 37.62±1.11 mg to 79.03±0.42 mg. For riboflavin in 5 mg tablets of five different companies, active ingredient was found in the range of 6.78±0.19 mg to 8.23±0.15 mg. In case of pyridoxine hydrochloride tablets, it was found that 20 mg tablet of one company contained 21.75±0.59 mg of active ingredient. Recovery experiments were done by spiking excipients of the respective medicines at three different concentration levels with 5 replicate studies. Correlation coefficients (r2) were found to be 0.99 for three vitamins and recoveries were 82.26±0.10, 99.61±0.06 and 109.91±0.12 for thiamine hydrochloride (vitamin B1), riboflavin (vitamin

B2), and pyridoxine hydrochloride (vitamin B6), respectively.

10. Md. Nazimuddin, MS (Graduation: 2015); Studies of Polyaromatic Hydrocarbons in Upstream Water Samples from Buriganga, Meghna, Turag Rivers and Gulshan Lake

Abstract

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in nature and widely found in plant, air, soil and water. Anthropogenic activities e.g. motor vehicles, industrial processes, domestic heating, waste incineration etc. are major sources of PAHs to the environment. Due to their carcinogenicity, the water pollution caused by PAHs is a great concern worldwide. Dhaka, the capital of Bangladesh with an inhabitant of 20 million people is vulnerable for the anthropogenic activities. High performance liquid chromatography coupled with fluorescence detector (HPLC-‐FLD) was used for the identification and quantification of anthracene, benzo[a]pyrene and fluoranthene in rivers (Buriganga, Meghna, Turag) and Gulshan lake water samples. Solid phase extraction technique using C-‐18 cartridge was used for the pre-‐concentration as well as extraction of PAHs from water. The linear correlation coefficient (r2) for anthracene, benzo[a]pyrene and fluoranthene were r2 ≥0.9943, r2 ≥0.9983 and r2 ≥0.9975, respectively. The limit of detection (LOD) for anthracene, benzo[a]pyrene and fluoranthene was found to be 200 ng/L, 0.63 ng/L, and 0.63 ng/L, respectively, whereas the limit of quantification (LOQ) was found to be 660 ng/L, 2.08 ng/L, and 2.08 ng/L, respectively. Five replicate recovery experiments for each of anthracene, benzo[a]pyrene and fluoranthene at a spiking level of 20 ng/L were done for the establishment and validation of SPE and HPLC-‐FLD method. The average percent recoveries of anthracene, benzo[a]pyrene and fluoranthene were found to be 91.38%, 95.72%, and 85.49%, respectively, and the RSD (%) values of anthracene, benzo[a]pyrene and fluoranthene were found to be ≤ 5.27 %, ≤ 17.55 %, ≤ 2.84 %, respectively. A total of twenty three water samples from the rivers (Buriganga, Meghna, Turag) and Gulshan Lake were analyzed and the results showed that, most of the samples contain significant amount of fluoranthene and the highest concentration of fluoranthene was found in the sample collected from Meghna River. None of the samples contained benzo[a]pyrene above the limit of quantification (LOQ) values. A number of samples were found to contain trace amounts of anthracene but the highest concentration of anthracene was found in the sample collected from Gulshan Lake.

11. Prithu Mondal, MS (Graduation: 2015); Chemical and Biological Activity Studies of Mushrooms

Abstract

Mushrooms are macrofungi belonging to the class Agaricomycetes with unique and visible fruiting bodies that usually grow above ground. Mycorrhiza-‐interactions between the fungal mycelia and plants roots are essentials for the functions in many terrestrial ecosystems. Mushrooms are rich in protein, minerals, vitamins and essential amino acids, and have been utilized for human

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consumption since ancient times as a normal part of the diet due to their desirable taste and aroma. Fresh naturally growing fruiting bodies of mushroom species namely Agaricus bisporus, Coprinus micaceus, Pleurotus ostreatus, Termitomyces clypeatus and T. heimii were collected from Dhaka University Campus in June 2014. Freeze-‐dried species were extracted separately with dichloromethane:methanol (1:1) and dried which was partitioned using hexane, dichloromethane and methanol. Silica gel column chromatography of hexane extract of T. heimii and dichloromethane extract of P. ostreatus afforded 2,4-‐di-‐hydroxy-‐9-‐octadecenoic acid and 2-‐hydroxy-‐9-‐octadecenoic acid, respectively. Water insoluble components are present in minor amounts in mushrooms and keep active roles by providing essential fatty acids and potential biological activities. The n-‐hexane extracts of mushrooms were analyzed for the presence of n-‐alkanes and fatty acid compositions. n-‐Alkanes were found in T. heimii only. Different extracts of mushrooms were evaluated for antimicrobial activity against a wide range of Gram-‐positive and Gram-‐negative bacteria and fungi, general toxicity and antioxidant activity.

12. Sharmin Sultana, MS (Graduation: 2016); Assessment of Chemical Constituents in Some Pesticides Treated and Untreated Vegetable Samples

Abstract

Pesticides are being widely used in Bangladesh in order to increase the production of crops to meet the high demand for a population of about 160 million. Among them organophosphorus, pyrethroids and carbamates, groups of pesticides are mostly used in vegetable fields. Of these three groups cypermethrin, emamectin benzoate, fenvalerate, diazinon and chlorpyrifos are randomly used in vegetable field. Excessive use of these pesticides has adverse effect on human health and environment in Bangladeh. The use of pesticides on crops, vegetables and fruits may change major (protein, starch, dietary fibers etc.) and minor (vitamins, minerals, carotinoids etc.) constituents which are very important for a healthy life. The present study is aimed to assess the chemical constituents of pesticide treated and untreated three vegetables i.e., pumpkin (Cucurbita pepo L.), eggplant (Solanum melongena L.) and bottle gourd (Lagearia sicreraria L.). Proclaim (Emamectin benzoate), ridomil (Mancozeb), sobicron (cypermethrin) were sprayed in the vegetable fields. All samples were collected from one of the largest vegetable growing area Nurundi, Jamalpur on 4th February 2016. Some samples which were collected before the application of pesticides and termed as untreated and in the same place pesticides (cypermethrin, avermactin benzoate and mancozeb) were applied to these vegetables and were collected 2 hours after application which are termed as treated samples. Except bottle gourd all three pesticides were applied individually for each sample. In bottle gourd, combination of three pesticides (cypermethrin, avermactin benzoate and mancozeb) was applied. Water content of the three pesticides (cypermethrin, avermactin benzoate and mancozeb) treated vegetables (pumpkin, eggplant and bottle gourd) and untreated samples were very similar i. e. , 91-‐94% whereas ash content of treated samples decreased in the case of cypermethrin and emamectin benzoate but increased in the case of mancozeb and mixed pesticide except pumpkin. Tannins, proteins, carbohydrates and saponins were found in all untreated and treated samples. All three untreated vegetable samples showed glycosides but not in treated except cypermethrin treated pumpkin. The protein contents in eggplant, pumpkin and bottle gourd were found 13.31%, 8.71% and 8.24%, respectively. All treated eggplant gave lower amount (10.12-‐13.17%) of proteins. Except emamectin benzoate treated pumpkin all treated pumpkin showed decreased level of proteins. Mixed bottle gourd sample showed increased percentage of proteins. On the other hand, the percentages of carbohydrate in untreated eggplant, pumpkin and bottle gourd had 0.83%, 0.45% and 1.1%, respectively. Percentage carbohydrate in the pesticide treated and untreated samples did not significantly change. Results showed that the amounts of protein were increased in the corresponding treated samples whose carbohydrates had decreased but these

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changes were not significant. Amount of elements in treated and untreated samples were more or less same except potassium. Zn, Mg, Mn, Cu, Fe, Ca and K contents were found in the range of 0.02-‐0.034, 0.10-‐6.20, 0.01-‐0.02, 0.01-‐2.50, 0.04-‐2.53, 2.03-‐8.70 and 10.27-‐41.29 mg/g, respectively. Trace amount of Pb, Ni and Co were not found to be present the vegetable samples.

13. Jahan Jenny, MS (Graduation: 2016); Nutritional and Phytochemical Study of Some Edible Vegetables

Abstract

Bangladesh is an agro-‐based middle-‐income country. Rice, pulses, maize, wheat, jute, potato, sugarcane, vegetable and tea are the major agricultural crops. In-‐spite of being eighth populous country of the world, Bangladesh is ensuring food security. Vegetables contain mineral, vitamins and other beneficiary constituents for a balanced diet. The nutritional value of vegetables may be affected by the application of pesticides (such as organophosphorus, pyrethroid, carbamates groups of pesticide etc.) which are indiscriminately used in the country to increase the crop productions. In order to study the effect of pesticides on the nutritional values of vegetables three vegetables i.e., bitter gourd (Momordica charantia L.), tomato (Solanum lycopersicum L.) and chili (Capsicum annum L.) were collected from vegetable growing field of Nurundi, Jamalpur. Two insecticides, sobicron (cypermethrin) and proclaim (emamectin benzoate) and one fungicide ridomil (mancozeb) were sprayed in the vegetable field. Some vegetables which were collected before application of pesticide (untreated) and two hours after spraying with pesticides. All samples were collected in Zip-‐lock bag and transported to the laboratory immediately. Water content of the three pesticides (cypermethrin, ridomil and emamectin benzoate) treated of the three vegetables (bitter gourd, chili, tomato) and untreated samples were very similar i.e., 84-‐93% whereas ash content of treated samples decreased in the case of cypermethrin and emamectin benzoate but increased in the case of mancozeb. The vegetable samples were screened for minor and major chemical constituents. Tannins, glycosides, saponins, proteins and carbohydrates were found to be present in untreated and pesticide treated vegetables except glycosides which were not found in treated vegetables. Percent carbohydrate in the pesticide treated and untreated samples were not significantly changed. In case of bitter gourd and tomato the protein content was slightly decreased whereas carbohydrate content was increased. Amount of K was the highest among all the elements measured in all the treated and untreated samples. Other elements such as Ca, Cu, Fe, Zn, Mg and Mn were same or similar in amount. The level of Ca, Cu, Fe, Zn, Mg, Mn and K were in the range of 0.95-‐4.1 mg/g, 0.009-‐0.79 mg/g, 0.05-‐1.14 mg/g, 0.01-‐0.13 mg/g, 0.10-‐3.40 mg/g, 0.003-‐0.15 mg/g and 15.67-‐26.49 mg/g. Number of sample size needs to be increased to verify the results. The results obtained is an affirmation of the use of these vegetables (treated with pesticide) and thus these vegetables may serve as sources of safe food for consumption.

14. Md. Amjad Hossain, MS (Graduation, 2016); Organochlorine pesticides and fatty acid composition in fresh water fish sample

Abstract

The aim of the present study was to determine the residual amounts of DDT and its metabolites and the fatty acid compositions of fish samples. Nineteen fish species were collected from Kangsha & Titas rivers and three cultured fish samples from Brahmanbaria. The fish samples were extracted by QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method and cleaned up with conc. H2SO4treatment, and cleaned extracts were analyzed by GC-‐ECD. For the analysis of fatty acid compositions oil was extracted then saponified with alcoholic NaOH and esterification with BF3-‐

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MeOH and were analyzed by GC-‐FID. Small size cultured Rui fish sample which did show presence detectable amount of DDT and its metabolites was used for the recovery experiments. Percent recovery was found in the range of 70-‐113. The residual amount of DDTs in twenty two fish samples were found to be in the range of 4.71-‐78.81ng/g. Amount of DDTs were found 54.34, 48.81, 62.09, 54.72,78.81, 60.07, 47.0, 42.7, 26.31,10.36, 25.32, 12.96, 20.10, 12.78, 17.65, and 4.71ng/g in gulsha, pabdha, baila, bacha, baim, small puti, tengra, chanda, kachki, boal, taki, chingri, mola, shole, shinh, and koi respectively in river fish samples and 8.58, 11.3 & 19.01incultured swarputi, karp and tilapia, respectively. However, the residual DDTs in all the fish samples were below maximum residue limit (MRL of DDTs in fish 5.0 mg/kg) according Codex Alimentarius Commission. The ratios of 4,4-‐DDT/DDTs were in the range of 0.03-‐0.44did not show fresh use of DDT in the country. Fatty acid compositions of fishes were determined in order to see the lipid profile. Varying amounts of saturated and unsaturated fatty acids were found in the analyzed 22 fish samples. Among the saturated fatty acids, Palmitic acid (7-‐50%), Stearic acid (6-‐72%), Myristic acid (0.50-‐50%) and Arachidic acid (1-‐18%) are predominantly found. Most of the analyzed fish contained slightly higher amount of saturated fatty acids than that of unsaturated. But in Boal (dorsal), Shole and Puti fish the amount of unsaturated fatty acids are higher than that of saturated. Among the unsaturated fatty acids monounsaturated Oleic acid (up to 56%), Palmotelic acid (up to 21%) and Elaidic acid (up to 38%) are higher than the other polyunsaturated fatty acids. 15. Md. Golam Kibria, MS (Graduation, 2016); Fatty acid compositions and bioaccumulation of

heavy metals in fishes of different trophic level

Abstract

Fish which is easily available and consumed every day in Bangladesh is one of the protein sources for a population of 160 million. It gives high-‐value amino acids, vitamins and minerals, and is an excellent sources of essential omega-‐3 fatty acids associated with many health benefits. Twenty four fish samples of different tropic level i.e., Omnivore, Herbivore and Carnivore were collected from two contaminated rivers, the Buriganga and the Meghna surrounding the Dhaka city. Among them Palmitic acid (10-‐52%), Stearic acid (2-‐59%), Myristic acid (2-‐20%) and Arachidic acid (up to 16%) are predominant. The amount of unsaturated fatty acid content is lower than saturated fatty acid. Among the unsaturated fatty acids mono-‐unsaturated Oleic acid (1-‐45%), Palmotelic acid (1-‐ 24%) and Elaidic acid (up to 32%) are higher than the other poly-‐unsaturated fatty acids. However, high consumption of some fishes can have adverse effects on human health due to bioaccumulation of heavy metals like Hg, Cd, Cr, As and Cd in fish muscles from the surrounding aquatic environment. In order to assess the accumulation of these metals were also studied. Samples were freeze-‐dried and digested by Microwave Digestion (HNO3-‐H2O2 ; 6:1 mixture) and finally analyzed by Atomic Absorption Spectroscopy (AAS) and Inductively Coupled Plasma Mass Spectrometry (ICP-‐MS). Heavy metals were detected in most of the samples in varying amount. The range of heavy metals concentration were found to be in the range of 0.14-‐3.18 mg/kg for Cr, 0.07-‐1.73 mg/kg for Hg, 0.01-‐0.50 mg/kg for Cd, 0.06-‐5.72 mg/kg for As and 0.04-‐0.67 mg/kg for Pb. Among the five heavy metals, bioaccumulation of As is predominantly higher in herbivorous Hilsha and Punti fishes than that of other metals.

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16. Md. Ahasan Ul Hoque Sakib, MS (Graduation, 2016); Investigation of Three Surfactants in Water by Spectrophotometric Method

Abstract

Surfactants are among the most commonly used cleaning agents in household and industrial purposes. Excessive use of surfactants generates some questionable environmental issues, such as surface water pollution, depletion of dissolve oxygen, several adverse effects on fishes, as well as human beings etc. In many developed countries, surfactants are considered as one of the pollutants for their less biodegradability in the environment and carefully monitored in the surface water. In Bangladesh, use of surfactants is increasing day by day and there is no monitoring system. In the present work, UV-‐Vis spectrophotometric methods were used for the quantification of three different surfactants, namely Sodium dodecyl sulfate (an anionic surfactant), Triton X-‐100 (a nonionic surfactant) and Cetrimonium bromide (a cationic surfactant). The anionic and cationic surfactants form colored complexes with cationic dye acridine orange (ACO) and anionic dye methyl orange (MO) respectively. These complexes were extracted with toluene and their absorbances were measured at 499 nm and 415 nm, respectively. Triton X-‐100 has λmax at 275 nm and can be measured directly by UV-‐Vis spectrophotometer. Identification was done making colored complexes for SDS and CTAB and quantification was carried out with respect to the standard calibration curve of the standards. The calibration curves were linear in the range of 0-‐2.5 ppm, 6.25-‐100 ppm and 3.12-‐25 ppm for Sodium dodecyl sulfate (SDS), Triton X-‐100 and Cetrimonium bromide (CTAB), respectively. The correlation coefficients were found to be 0.994, 0.992 and 0.976 for SDS, Triton X-‐100 and CTAB, respectively. Samples were collected from twenty different locations. Out of these twenty samples, SDS was found in all the samples in the range from 0.018-‐1.499 ppm. Triton X-‐100 and CTAB were not detected in any of the collected samples. The present methods are much easier, less time consuming and requires less toxic chemicals which are applicable to analyse surfactants in water both qualitatively and quantitatively.

17. .Md. Mazharul Islam, MS (Graduation: 2017); Organochlorine Compounds and Arsenic, Cadmium and Lead in Marine Fish Samples of Bangladesh

Abstract

Bioaccumulation of organochlorine compounds and heavy metals in marine fishes occurs as a result of environmental pollution which causes toxicity not only in human beings but also to the plants and animals. Generally, the presence of contaminants in fish is a result of human activities such as industrial and agricultural wastes. The objective of the present study is to analyze different marine fish samples (n=25) of Bangladesh. The collected fish samples are Eleutheronematetradactylum (Lakkha), Metapenaeusmonoceros (Chingri),Latescalcarifer (Koral), Harpodon nehereus (Loitta), Pampusargenteus (Shadachanda), Setipinnaphasa (Faissa), Leiognathusequulus (Tekchanda), Tenualosailisha(illish), Megalapsiscordyla (Surma), Parastromateusniger (Kalochanda), Coiliaramcarati (Ulua), Otolithoidespama (Poa), Ariusmaculatus (Riksha), Paraplagusiabilineata (Pata), Strongyluraleiura (Tuitta), Platycephalusindicus(Undurabailla). These samples arecollected from three different local markets for the presence of organochlorine compounds (such as 4, 4/-‐DDE, 4, 4/-‐DDD, 2, 4/-‐DDT, 4, 4/-‐DDT) using solid phase extraction (SPE) method andQuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method through gas chromatograph equipped with electron capture detector (GC-‐ECD) and the level of DDTs in fish sample was found at safe level. Heavy metals such as arsenic (As), cadmium (Cd) and lead (Pb) were analyzed quantitatively using atomic absorption spectrophotometer (AAS). Organochlorine pesticides are responsible for different environmental consequences. In this study, the concentration level of organochlorine compounds is

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investigated in fish samples of Bay of Bengal. This also indicates the pesticides accumulation and contamination of Bay of Bengal. Muscle of fish samples were dried, digested by Microwave Digestion (HNO3-‐H2O2; 4:1 mixture, 10 mL each) maintaining temperature between 140-‐180 0C for 30 min and finally analyzed by AAS. The levels of heavy metals varied significantly among fish species and showed that arsenic (As), cadmium (Cd) and lead (Pb) levels of mean values 0.12, 0.22 and 0.99 mg/kg, respectively. The range of arsenic (As), cadmium (Cd) and lead (Pb) in dried samples were <0.10 to 0.18, < 0.10 to 0.97 and 0.25 to 1.68 mg/kg respectively. Most part of the values was below the permitted limit set by FAO/WHO 2004 as well as Food Act 1983 and Food Regulations 1985, except cadmium (Cd) in Chingri (M. monoceros) that is present in excess amount permitted by FAO/WHO 2004. This may be due to textile industries, refineries, automobiles, leather industries that discharge their untreated waste products. Cadmium is associated with nephrotoxic effects particularly long term exposure may cause bone damage. The more we consume fish that shows the type of results above the more exposed to bioaccumulation and its consequences. Although heavy metal levels are below the recommended value for most of the samples, excess intake of contaminated fishes may impose a health risk factor, especially fishers and coastal communities who may eat fishes every day.

Robiul Islam, MS (Graduation: 2017); Studies of Fatty Acid Compositions and Level of Cr and Hg in Marine Fish Samples

Abstract

Marine fishes provide a healthful source of dietary protein and are usually high in nutrients and fatty acids specially Omga-‐3 and omega-‐6.There are a lot of evidences of beneficial effects of fish consumption in coronary heart diseases, stroke, growth-‐development etc. This study was designed to provide a comparable data of both saturated and unsaturated fatty acids available in the marine fish samples. A total of 25 marine fish samples of 16 different species were collected from three different local markets of Chittagong. Fish oil was extracted by Solid phase dispersion method. Saponification and esterification was carried out by AOAC reference procedure with some simple modifications. The fatty acid methyl esters(FAMEs) was then analyzed by Gas chromatography equipped with Flame ionization detector (GC-‐FID).Fatty acids were identified using the retention time of the standards. Among the 16 species of marine fishes saturated fatty acids i.e;Palmitic acid (1.41-‐39.27%), Stearic acid (0.47-‐18.89%), Myristic acid (2.67-‐82.77%) and Arachidic acid (3.98-‐43.28%) were predominant. Among the unsatrurated fatty acids (MUFAs and PUFAs) Oleic acid (5.03-‐46.27%), Palmitolic acid (2.9-‐ 14.69%), Elaidic acid (0.61-‐6.28%),Eruic acid (1.03-‐28.94%) and Linoleic acid(1.3-‐36.83%) were found to be predominant. In this study Faissa(Setipinnaphasa) was found the most fatty fish and Pata(Paraplagusia bilineata) was the least one.These fishes are the significant source of protein, vitamines, minerals and have nutritious value. But currently they are affected by industrialization,Shipping, Coastal activity and pollution which results increased concentrations of heavy metals. In this study,concentration of chromium (Cr) and mercury(Hg) in the muscle of 19 marine fish samples were determined by using Atomic Absorption Spectrometer (AAS).The level of heavy metals(Cr and Hg) was varied significantly among the species.The analysis of fish samples for heavy metals shows Cr and Hg level of mean values 2.97 and 0.93mg/kg respectively. For the dried fish samples the range of Cr was 1.75 to 5.41 mg/kg and Hg was < 0.10 to 3.17 mg/kg. Most part of the values was below the permitted limit set by FAO/WHO 2004 as well as Food Act 1983 and Food Regulations 1985. Although heavy metal levels are below the recommended value for most of the samples, excess intake of contaminated fishes may impose a health risk factor, especially fishers and coastal communities who may eat fishes up to daily basis.

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18. Anowar Hossain Khan, MS (Graduation: 2017); Organochlorine Compounds and Heavy Metals in Sediment Samples from Coastal Area of Bangladesh

Abstract

Sediment is a very important part of our environment. If it gets polluted than contamination can threaten creatures in the benthic environment, exposing worms. Some kinds of toxic sediments kill benthic organisms, reducing the food available to larger animals such as fish. Some contaminants in the sediment are taken up by benthic organisms in a process called bioaccumulation, finally biomagnification takes place. In order to evalute the level of contaminates such as heavy metals and DDTs, fifteen sediment sampls from Karamjal point, Mongla Port, Rupsha river, surrounding Sundarban were collected on 14 May 2016. Another eighteen sediment samples were collected from Bhairab River, Mayur River and Rupsa River on 31 March 2017 and studied. All samples were stored in a refrigerator maintaining temperature -‐20˚C until analysis. Extraction was done by QuEChER’s method using acetonitrile as solvent. Anhydrous of magnesium sulphate and sodium chloride were also added and finally clean up by PSA and anhydrous magnesium sulphate, re-‐constituents in n-‐hexane for analysis of residual amounts of DDTs and its metabolite (4,4-‐DDE and 4,4-‐DDD) by GC-‐ECD (Gas chromatohgraphy-‐electron capture detector). The linearity of the method was well demonstrated over concentration range of 0.05-‐1000 μg/L with an r2 (regression coefficient) value in the range of 0.9922-‐ 0.9984 which is higher than the value recommended by the Codex guideline (r2 =0.95). The percentage of recovery was found in the range of 73-‐114.3% which were in the range 70-‐120 and acceptable. The residual amounts of DDTs in analyzed thirty two samples were found to be in the range of 0.18-‐19.16 μg/L.

For heavy metals analysis sediment samples were freeze dried and analyzed by atomic absorption spectrophotometer (AAS). Five different metals (Pb, Cr, Cd, Sn and As) were studied. Only arsenic was found in all samples with the range of 4.08-‐32.15 mg/kg, other metals (Pb, Cr, Cd, Sn) were found below detection limit.

19. Jannatun Noor Jeouty, MS (Graduation: 2017); Studies of Microplastic, Organochlorine Compounds and Toxic Metals in Marine Fish Samples of Sundarban Region

ABSTRACT

Sundarban, the largest mangrove forest of the world lies in the vast delta on the Bay of Bengal and as different chemical contaminants float in the world’s oceans so there is huge possibility for the aquatic fish samples of Sundarbans to be exposed to those contamination. In this study, amounts of toxic metals, DDT and its metabolites and method development for determination of the presence of microplastic in the aquatic fish samples of the coastal area near the Sundarbans have been studied. Eighteen species of marine fish samples were collected from a local bazaar near Poshur river of Mongla, Khulna for this study. Nowadays there is growing concern over the ecological risk of microplastics among regulators, the scientific community and the public. The use of plastics has gradually increased since the middle of the last century. The Gastrointestinal tracts of the fishes were digested in 10% KOH at 60º for 12 hours followed by filtration and drying for finding the presence of microplastic which was analyzed by FT-‐IR and analyzing the data no plastic contamination was found in the samples. Fillet of fish samples were also extracted with ethyl acetate followed by clean up with silica-‐sulphuric acid and analyzed by gas chromatography (GC) equipped with electron capture detector (GC-‐ECD) for the presence of organochlorine pesticides. The limit of detection of 2,4´-‐DDT, 4,4´-‐ DDT, 4,4´-‐ DDD, 4,4´-‐DDE were 0.025 ng/g and linear correlation coefficient (r2) were 0.998. The residual amount of DDTs in analyzed eighteen fish samples were found to be in the range of 1.01-‐25.50 ng/g. The highest value was found in Datone

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fish (25.50 ng/g) and lowest value was found in Ranibou (1.01 ng/g). However, the residual DDTs in all the fish samples were below maximum residue limit (MRL of DDTs in fish 5.0 mg/kg), Codex Alimentarius, 1993. Statistical analysis was carried out to evaluate risk based consumption limit for the DDTs in contaminated fishes. High consumption of fishes can have adverse effects on human health due to bioaccumulation of toxic metals like Hg, Cd, Cr, As and Pb in fish muscles from the surrounding aquatic environment. In order to assess the accumulation of these toxic heavy metals fish samples were studied. Samples were digested after freeze-‐drying and finally analyzed by Atomic Absorption Spectroscopy (AAS). Heavy metals were detected in all the samples in varying amounts. The range of toxic metals concentration was found to be in the range of 1.09 to 1.21 mg/L for Cd, 0.72-‐5.00 mg/L for Cr, 0.72-‐5.00 mg/L for As, 0.01-‐0.93 mg/L for Hg and 0.14-‐5.24 mg/L for Pb. Among the five heavy metals, As is predominantly highest in one sample (Mochontengra) and the amount is 13.5 mg/L.

20. Rehnuma Tabassum, MS (Graduation: 2017); Study of Water Quality Parameters and 9999Environmental Contaminants in the Aquatic Ecosystem of the Bay of Bengal

Abstract

Bangladesh has a land area of 144,054 km² and being land-‐lockedon three sides, it is in the south alone that it has a coastline, the Bay of Bengal.The coastal areas of Bangladesh include 60% of the Sundarbans. The Sundarban mangrove forest in thesouthwestern part is the largest single compact mangrove resource in the world and has a population of 35.1 million. A large amounts of contaminants pass through the sensitive environment of the Sundarban. Currently marine debris are among the main environmental challenges globally.Heavy metals, Persistent organic pollutants (POPs) such as organochlorine pesticidesetc.may enter the ecosystem.The objective of present study is to gather preliminary data about the environmental contaminants which may affect the ecosystems of the Bay of Bengal. About 52 water samples were collected from Karamjal point of Sundarban coastal region, Poshurriver, Mongla port including Rupsa river, Bhairab river and Moyur river in Khulna city.The common water quality parameters such as pH, electrical conductivity, TOC, DO, BOD, nutrients ions were examined to discuss the quality of water andmeasure the pollution. The value of pH and conductivity vary within 7.70-‐9.02 and 9.14-‐21.3mScm-‐1respectively. The TOC, DO and BOD levels were found in the range of 2.243-‐8.172 ppm, 1.39-‐7.52 ppm, and 0.66-‐35.3 ppm respectively. The water samples wereanalyzed for 11toxic metals e.g., Hg, As, Pb, Cr, Cd,Cu, Zn, Mn, Fe, Se and Ni using atomic absorption spectroscopy (AAS) instrument. The contamination due to heavy metal trace elements was found in very insignificant level ( As < 0.005 mg/L, Hg <0.001mg/L, Cr < 0.005 mg/L, Cd < 0.002 mg/L, Pb<0.01 mg/L), Cu (BDL), Zn (BDL), Mn (0.015 ppm), Fe (0.039 ppm), Se (0.0017 ppb) and Ni (0.0004 ppb).The PCB analysis was carried out by extraction using dichloromethane and sodium chloride followed by reconstitution in n-‐hexane and then analyzed bygas chromatography equipped with electron capture detector(GC-‐ECD).The reason of low contaminants might be attributed that the current of the river does not let the contaminants to accumulate or concentrate in watercourse. Since the tidal flow of the river gets mixed with sea, the pollutants become diluted. Therefore,constant monitoring of water quality of the Bengal coast is needed to record with aview to minimize the risk of health of the population and the detrimental impactson the aquatic environment.

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21. Most. Salma Khatun, MS (Graduation: 2017); Bioactive Secondary Metabolites from Plant Averrhoa bilimbi L.

Abstract

Averrhoabilimbi L., belonging to the Oxalidaceae family is native to Bangladesh and locally known as Bilombo. The plant was reported to have medicinal properties like antidiabetic, antioxidant, cytotoxic etc. The present study was aimed to screen phytochemical properties including isolation of secondary metabolites, and performing brine shrimp lethality bioassay and antimicrobial activity of the leaves of A. bilimbi. The leaves of A. bilimbi (700 g) was extracted with 80% ethanol and 104 g of crude extract was found. The crude ethanolic extract was dissolved in water and partitioned successively with dichloromethane (DCM), ethyl acetate and n-‐butanol. The phytochemical screening of the extracts of leaves of A. bilimbi revealed the presence of phytochemicals such as tannins, saponins, flavonoids, steroids, terpenoid and cardiac glycosides. Among all extracts, only DCM (20 g) extract was fractionated by normal phase silica gel column chromatography for isolation of compounds. By repeated column chromatography two compounds (labeled as B-‐1 and B-‐2) which gave single spots on thin layer column chromatography were isolated as pure compounds. Compounds B-‐1 and B-‐2 were characterized as a fatty alcohol (1-‐Eicosanol) and a phytosterol (β-‐sitosterol), respectively by FT-‐IR, 1H-‐NMR and 13C-‐NMR studies. Brine shrimp lethality bioassay of n-‐hexane extract and DCM extract was carried out. The LC50 values of n-‐hexane and DCM extracts were found to be 6.84 and 9.51 µg/mL, respectively. Both the extracts showed moderate activity. The antimicrobial activities of n-‐hexane extract and DCM extracts were evaluated by disk-‐diffusion method against different gram positive and gram negative bacteria and fungi. But both of the extracts exhibited no inhibitory activity against tested bacteria and fungi.

22. Rehnuma Tabassum, BS Honours (Graduation: 2015); Estimation of Anionic Surfactant Sodium Dodecyl Sulphate in water from Ramna Lake, Hatirjheel Lake and Dharla River of Dhaka city using Spectrophotometric Technique.

Abstract

Surfactants are compounds that lower the surface tension or interfacial tension between two liquid phases or between a solid phase and a liquid phase. Chemically there are four types of surfactants and anionic surfactant is one of them. Anionic surfactants are surface active compounds being composed of hydrophobic alkyl chain which is connected to one or two hydrophilic groups. Most of the surfactants are used in the form of detergents powder and is directed into the aquatic environment. They are generally considered as emerging pollutants due to causing toxic effects on many different organisms in ecosystem. In the present work, we followed a simplified spectrophotometric method for the determination of anionic surfactant-‐Sodium Dodecyl Sulfate (SDS) based on the formation of a yellow colored Sodium Dodecyl Sulfate-‐Acridine Orange (SDS–ACO) complex which is extracted in toluene. Four water samples from two lakes and one river were analyzed. The absorbance of the complex in toluene layer is measured at wavelength of 499 nm. The calibration curve was linear in the range of 0-‐2.5 ppm of SDS concentration and the correlation coefficient (r2) was 0.994. The presence of SDS was found in varying amounts ranging from 0.17 to 0.5 µg/mL. The water collected from Ramna Lake and Hatir Jheel lake (west) contained average amount of 0.175 µg/mL and 0.203 µg/mL surfactant respectively and the water samples collected from Hatir Jheel lake (central) and Dharala river contained average amount of 0.326 ppm & 0.425 ppm surfactant respectively. The maximum limit for anionic detergent is standard 0.5 mg/L for drinking water and can be up to 1.0 mg/L for other purposes.

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23. Anowar Hossain Khan, BS Honours (Graduation: 2015); Evaluation of Diclofenac Sodium in Pharmaceutical Products by UV-‐Visible Spectrophotometer.

Abstract

Diclofenac sodium, a nonsteroidal anti-‐inflammatory drug (NSAID) is widely prescribed for the treatment of rheumatoid arthritis, osteoarthritis, musculoskeletal injuries and post surgery analgesia. A rapid and simple UV-‐Visible spectrophotometric method is developed for the determination of diclofenac sodium in tablets produced by different pharmaceutical companies of Bangladesh. Tables from seven different companies of three different batches (n=21) were collected. Five tablets of each batch were ground together and extracted with methanol followed by centrifugation and filtration using 0.25 μm syringe filter and evaluated by UV-‐Visible spectrophotometer. The wavelength of maximum absorption of diclofenac sodium (methanol) was 282 nm. The correlation coefficient (r2) of standard calibration curve was 0.997. Amount of diclofenac sodium in 50 mg tablets of seven different companies were found in the range of 47.91 ±0.90 mg to 58.52 ± 0.41 mg. Described method for determination of diclofenac sodium is satisfactory for wide range of concentration (2-‐50 μg/mL). Recovery experiment was carried by spiking the standard diclofenac sodium in the excipients used in tablets at two different concentration levels (10 and 20 μg/mL ) with eight different replicates and were 103.3% and 102.5% for 10 and 20 μg/mL, respectively. 24. Robiul Islam, BS Honours (Graduation: 2015); Quantitative estimation of sodium benzoate and

caffeine in popular soft drink: 7UP by UV-‐Visible Spectrophotometer

Abstract Sodium benzoate and caffeine are extensively used as preservative and stimulant, respectively in food processing for maintaining food quality and its characteristics. However, these substances are subject to regulation, since their excessive or inappropriate use may cause food safety problems and introduce a risk factor, especially for children and pregnant women. A rapid and simple UV-‐Visible spectrophotometric method is used for the determination of sodium benzoate and caffeine in popular soft drink i.e., 7UP (lemon). The standard reference samples of sodium benzoate and caffeine were used for identification and quantification of the preservative and the stimulant, respectively. The soft drink samples were degassed, extracted with HPLC grade water and cleaned up to study their contents. The wavelength of absorption maxima (λmax) of sodium benzoate was 224 nm while 272 nm for caffeine. The correlation coefficients (r2) of the standard calibration curves were 0.997 and 0.986 for sodium benzoate and caffeine, respectively. The average quantities of caffeine in the analyzed beverages were found in the range of 20-‐30 mg/L. Sodium benzoate contents were 140-‐160 mg/L. The repeatability of the method was very satisfactory with value of RSD ≤ 0.05% for caffeine and RSD ≤ 2.88% for sodium benzoate. The maximum amount of caffeine in carbonated beverages is limited to 0.02% (US FDA, 2006) and Codex Alimentarius limits the amount of sodium benzoate to 0.05 to 0.1%. However, the content of sodium benzoate and caffeine were found below the above maximum limits. The method is sufficiently sensitive and reproducible for routine analysis of sodium benzoate and caffeine in soft drinks.

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25. Md. Mazharul Islam, BS Honours (Graduation: 2015); UV-‐Visible Spectrophotometric Studies for Assessment of Sodium Benzoate and Caffeine Content in Sprite Sample

Abstract

The main aim of this work is to determine the preservative and central nervous system (CNS) stimulant contents of commercially available soft drinks in Bangladesh. Quantitative analysis of sodium benzoate and caffeine were performed by a double beam UV-‐Visible spectrophotometer. Analytical standard for reference, sodium benzoate and caffeine was collected from Drug Administration Bangladesh and stored in a vial with wrapping at 00C in a refrigerator. The wavelength of absorption maxima (λmax) of sodium benzoate was 224 nm while 272 nm for caffeine. The correlation coefficients (r2) of the standard calibration curves were 0.997 and 0.986 for sodium benzoate and caffeine, respectively. The carbonated soft drinks (sprite) showed Sodium Benzoate levels in the range of 181ppm to 191ppm, whereas the caffeine levels in the range of 22ppm to 30ppm. The maximum amount of caffeine in carbonated beverages is limited to 0.02% (US FDA, 2006) and Codex Alimentarius limits the amount of sodium benzoate to 0.05 to 0.1%. The sodium benzoate and caffeine contents in sprite samples analyzed in this study are above the maximum levels. The method is sufficiently sensitive and reproducible for routine analysis of sodium benzoate and caffeine in soft drinks. 26. Salma Akter Mou, BS Honours (Graduation: 2016); Studies of Food Preservatives and Color

additives in orange Jelly Samples by UV –visible spectrophotometer

Abstract

Sodium benzoate and yellow 6 contents in different orange jelly samples were determined using UV-‐visible spectroscopic method. Commercially available orange jelly contain a range of sodium benzoate and yellow 6 which are harmful for us above allowed limit. Objective of present work is to find out the amount of sodium benzoate and yellow 6 content in commercial orange jelly. For these purposes fifteen samples of 3 different brands of different batches were collected from different market of Dhaka city. The calibration curves for standard sodium benzoate and yellow 6 were prepared using water as a solvent. Calibration curve for sodium benzoate and yellow 6 were linear and the correlation coefficients of the calibration curve were found to be 0.999 and 0.991 for sodium benzoate and yellow 6 respectively. All orange jelly samples contain sodium benzoate in a range of 160 mg/L to 289 mg/L. Yellow 6 content is 0.011% to 0.014%.Out of 15 samples 10 samples contain yellow 6.Physiochemical characterization of jelly samples e.g. pH, acid value, iodine value were also studied. pH of the orange jelly of different brand were found below 3.00, ash content and water were found in the range of 0.19-‐0.99% and 23.76-‐50.50%, respectively. Iodine value and acid value were determined using titrimetric method. Iodine value and acid value were found to be in the range is 0.115-‐0.268 and 3.190-‐3.995 respectively. Total carbohydrate content was determined by modified molisch’s method. Total carbohydrate content of orange jelly samples is from 50.72-‐83.89%. The total solid in commercial brand samples were found to be in the range of 70-‐90 g/L, respectively. Deionized water was used as control Low RSD value, sensitivity and reproducibility of this method show that these methods can be used for routine analysis of sodium benzoate and yellow 6 content in orange jelly.

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27. Tanzina Akther, BS Honours (Graduation: 2016); Study of Physiochemical Properties and Analysis of Sodium Benzoate, Caffeine in Jam Samples

Abstract

Artificial color and synthetic sweetener are added in the commercial jam in addition to natural fruit juice to make the jam more attractive, tasty and this helps the commercial producer to make more profit. Some of the additives are beneficial for health and some are hazardous. The objective of this present study was to determine the presence of additives and preservatives in mixed fruit jam samples which were collected from different places of Dhaka city. Analyses of sodium benzoate and caffeine were carried out in twelve samples by UV-‐visible Spectrophotometric method. The calibration curves for standard sodium benzoate and caffiene were prepared using water as a solvent. The correlation coefficient for sodium benzoate and caffeine were found to be 0.999 and 0.986, respectively. Among 12 samples, five samples contain sodium benzoate in the range of 254-‐400 mg/L and four samples contain caffeine in the range of 43.7-‐111.8 mg/L. The highest amount of sodium benzoate and caffeine were found in sample J2, Pran (400.4 mg/L) and J11, Ahmed (111.7 mg/L), respectively. Iodine value and acid value were determined in three samples by titrimetric method. Iodine value and acid value were found to be in the range is 0.137-‐3.144 and 2.92-‐3.89 respectively. Some physiochemical properties like ash content, water content and total carbohydrate were determined. Carbohydrate was determined by phenol-‐sulfuric acid method. Water content and ash content are in the range of 29.25-‐46.22% and 0.20-‐0.49% respectively.

28. Mafizul islam, BS Honours (Graduation: 2016); Quantitative Estimation of Methyl Paraben in

Chilli Sauce by UV-‐Vis Spectrophotometer

Abstract

A rapid and simple UV spectrophotometric method was applied for the determination of the content levels of some food additives like methyl paraben. For this purposes 7 different batches of commercially available chill sauce were collected from Dhaka city markets. The analytical measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives with the permissible levels .The UV-‐Visible absorption maxima for Methyl paraben is 255nm. The standard sample of Methyl paraben was used to make calibration curve and overlain curve. The results obtained from this study indicated that the quantity levels of Methyl paraben in the analyzed Chilli sauce were found in the range of 39.89mg/L-‐118.06mg/L. The highest amount of methyl paraben was e found in the samples shezan and Ahmed respectively. The total carbohydrate of the seven samples also determined by phenol -‐sulfuric acid method and it is found to be in the range of 42.19mg/L-‐22-‐45mg/L. This is an easy and cost effective method to determine the presence of Methyl paraben in Chilli sauce and this method can be used for other food stuffs.

29. Saiful Islam Bhuyian, BS Honours (Graduation: 2016); Studies of physico-‐chemical properties of orange soft drinks and determination of food additives by UV-‐visible spectrophotometric method

Abstract

A rapid and simple UV spectrophotometric method is used for the determination of the content levels of sodium benzoate and yellow 6 in 14 different batches of orange soft drink samples

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commercially available in Dhaka local markets in Bangladesh. The standard samples of sodium benzoate and yellow 6 were used to make calibration curves and the correlation coefficient of the calibration curves were found to be 0.9993 and 0.9953 for sodium benzoate and yellow 6 respectively. These measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives with the permissible levels. The wavelength of absorption maxima was 225 nm for Sodium benzoate and 482 nm for yellow 6. Beer’s law is obeyed in a concentration range of 1 -‐ 100 μg / mL and 10-‐70 μg / mL for Sodium benzoate and yellow 6 respectively. The results obtained from this study indicated that the average quantity levels of yellow 6 is and sodium benzoate in the analyzed beverages were found in a range of 2.90 mg/L to 162 mg/L and 94 mg/L to 387 mg/L respectively. The reproducibility, repeatability of the method were very satisfactory with low value of RSD in a range of .05 to 4.17% and 0.05 to 4.17%. The present method has been successfully applied to the determination of Sodium benzoate and Yellow 6 in various batches of commercial brand of orange soft drinks

30. Tofael Ahmed, BS Honours (Graduation: 2016); Studies of physiochemical properties of orange juice and determination of yellow 6 by UV-‐visible spectrophotometric method

Abstract

Orange juices are very popular all over the world including Bangladesh. Yellow 6 (Disodium 6-‐hydroxy-‐5-‐(4-‐sulfophenyl) azo-‐2-‐naphthalenesulfonate) was used in food and drinks as color additives earlier but due to its adverse effects on human health, yellow 6 has been banned in many countries. However, this harmful color additive yellow 6 is being used in commercial orange juices in Bangladesh. Seven commercial brand yellow colored orange juices were analyzed to find out the presence of yellow 6. Juice prepared from fresh fruits was used as control. The UV-‐visible absorption maxima of yellow 6 is 429 nm. The amount of yellow 6 in commercial brands orange juices was determined by UV-‐visible spectrophotometer. Out of seven samples, six juice samples were found to contain yellow 6 in the range of 0.028-‐0.100 mg/L.

31. Saiful Islam, BS Honours (Graduation: 2016); Determination of methyl paraben and lycopene in tomato ketchup by UV-‐Visible spectrophotometric method

Abstract

A rapid and simple UV spectrophotometric method was applied for the determination ofsome food additives like methyl paraben and lycopene. For this purposes 10 different batches of commercially available tomato ketchup were collected from Dhaka city markets. The analytical measurements were undertaken primarily to assess the compliance of content levels of the investigated food additives with the permissible levels .The UV-‐ Visible absorption maxima for methyl paraben is 255nm and for lycopene is 472nm and 502nm in hexane. The standard sample of methyl paraben was used to make calibrationcurve and overlain curve. The results obtained from this study indicated that the quantity levels of Methyl paraben and lycopene in the analyzed tomato ketchup were found in the range of 39.89 -‐118.06mg/L and 10.56 -‐ 20.12mg/100 g, respectively . The highest amount of methyl paraben and lycopene were found in the samples shezan and ahmedrespectively. The total carbohydrate of the ten samples also determined by phenol –sulfuric acid method and it is found to be in the range of 7.77 -‐ 30.61 g/L . This is an easy and cost effective method to determine the presence of methylparaben and lycopene in tomato ketchup.

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32. Md. Ahsan Habib Khandakar, BS (Graduation-‐2017), Studies of Presence of Allura Red (Red-‐40) in Watermelon by UV-‐Visible Spectrophotometry

Abstract

Artificial food colors (AFCs) are used to color many beverages, foods, and sweets throughout the world. Some of these are beneficial for health and some are hazardous. In watermelon lycopene is responsible for red colour. Recently it has been reported in newspaper that an artificial dye like allura red (red-‐40) is injected to watermelon to make it more attractive which is very much toxic for our health. Allura red is a red azo dye that comes from petroleum distillates or coal tars. It is a derivative of naphthalene. According to the Center for Science in the Public Interest, Red 40 and other AFCs can cause allergic reactions in some people. It has been shown to cause hyperactivity in children. Experts also suspect it to be cancer causing. To ensure this whether the red-‐40 is injected or not the samples (controlled, spiked, market) were studied by UV-‐Vis spectrophotometry. Allura red is a water soluble dye which has absorption maxima at the wavelength about 507 nm. By taking the UV spectrum of the aqueous part of the watermelon extract the presence of allura red can be identified. The samples that were collected from the agricultural field was free from Allura red, hence they had no absorption maxima near 507 nm wavelength in the aqueous portion .Then the field samples that were spiked with Allura red. It resulted in absorption maxima near 507 nm wavelength in the aqueous portion. All the market samples collected from the different markets of Dhaka city were analyzed by UV spectrophotometry. They did not result in any absorption maxima near 507 nm wavelength in the aqueous part. Hence, it can be concluded that the market watermelons are free from Allura red. This method is easy, less time consuming and less expensive. The method can be used in future for any suspected watermelon is adulterated with Allura red.

33. Tanhaul Islam, BS (Graduation-‐2017) Studies of Presence of Erythrosine (Red 3) in Watermelon by UV –Visible Spectrophotometry

Abstract

Watermelons are one of the most treated summer fruits in our country. In the recent years, alarming news has been spread saying that watermelons are being adulterated with food dyes like Erythrosine (Red 3). The samples (controlled, spiked, market) were studied by UV-‐Vis spectrophotometry to find out the presence of Erythrosine (Red 3). Erythrosine is a water soluble dye which has absorption maxima at the wavelength about 530 nm. By taking the UV spectrum of the aqueous part of the watermelon extract the presence of Erythrosine can be identified. The samples that were collected from the agricultural field was free from Erythrosine, hence they had no absorption maxima near 530 nm wavelength in the aqueous portion .Then the field samples that were spiked with Erythrosine. It resulted in absorption maxima near 530 nm wavelength in the aqueous portion. All the market samples collected from the different markets of Dhaka city were analyzed by UV spectrophotometry. They did not result in any absorption maxima near 530 nm wavelength in the aqueous part. Hence, it can be concluded that the market watermelons are free from Erythrosine. This method is easy, less time consuming and less expensive. The method can be used in future for any suspected watermelon is adulterated with erythrosine.

34. Shatabdi Roy, BS (Graduation-‐2017), Studies of Synthetic Color Additive in Laddu

Abstract

Yellow 6 and yellow 5 contents in different sweet samples (laddu) were determined using UV-‐

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Visible spectroscopic method. Commercially available sweets (laddu) contain a range of yellow 6 and yellow 5 which are harmful for us above limit. Objective of present work is to find out the amount of yellow 6 and yellow 5 content in commercial laddus. For these purposes thirteen samples of 11 different brands of different batches were collected from different shops in Dhaka city. The calibration curve for standard yellow 6 and yellow 5 were prepared using water as a solvent. Calibration curve for yellow 6 and yellow 5 were linear. The correlation coefficients of the calibration curve were found to be 0.998 and 0.999 for yellow 6 and yellow 5 respectively. Out of thirteen samples 4 samples contain yellow 6 in the range of 4.462 mg/L to 86.308 mg/L and 5 samples contain yellow 5 in a range of 18.333 mg/L to 115.667 mg/L. Four samples did not contain any yellow 6 as well as yellow 5. We used distilled water because of its sensitivity and is helped to give a clear solution of samples. By the help of UV-‐Visible spectroscopic method we easily find out the absorbance of that clear solution using distilled water as a reference. The abosorbance detected the yellow 6 and yellow 5 in different samples and shows that the methods can be used for routine analysis of yellow 6 and yellow 5 content in laddus. 35. Forkan Saroar, BS (Graduation-‐2017), Studies of Synthetic Colorants in Sweet (Laddu)

Abstract

Sweet laddus are very popular all over the South-‐Asia including Bangladesh. Yellow-‐6 (Disodium 6-‐hydroxy-‐5-‐(4-‐sulfophenyl) azo-‐2-‐ naphthalenesulfonate) Yellow-‐5 (trisodium 1-‐(4-‐sulfonatophenyl)-‐4-‐(4-‐sulfonatophenylazo)-‐5-‐pyrazolone-‐3-‐carboxylate) were used in food and drinks as color additives earlier but due to its adverse effects on human health, Yellow-‐6, Yellow-‐5 have been banned in many countries. However, these harmful color additives Yellow-‐6 and Yellow-‐5 are being used in commercial drinks and sweets in Bangladesh. To identify and quantify Yellow-‐6 and Yellow-‐5 in locally made laddu, Twelve yellow colored brand sweets were analysed by thin layer chromatography (TLC) and UV-‐Visible Spectrophotometer. Absorption maxima of the two colorants were measured at 480 nm and 423 nm, respectively. Out of 13 samples, 8 samples were found to contain whether Yellow-‐5 in the rank of 18-‐136 ppm or Yellow-‐6 in the rank of 4-‐74 ppm 36. Elias Ahmed, BS (Graduation-‐2017), Phytochemical investigation of Morus alba L.

Abstract

Bangladesh is blessed with various plants, herbs and creepers by the nature and most of them are bioactive having medicinal importance and therapeutic value. Morus alba L. belongs to the Moraceae family (Locally known as tut tree) is generally distributed almost all over the world. It is widely used in folk medicine in Bangladesh as well as in Indian subcontinent. M. alba has been collected from Modhupur,Tangia l district of Bangladesh. The collected leaves of the plant was cleaned thoroughly and dried in open air and then below 40°C in an oven. The dried plants were grinded to powder by a grinder and the powder was stored in an air tight bottle before investigations. The powdered leaves of the plant was extracted with aqueous 80% ethanol. The ethanol extract was concentrated to dry mass using rotary evaporator below 40 °C. The dry mass of ethanol extract was dissolved in water and it was partitioned successively with DCM, ethyl acetate and finally with n-‐butanol using separatory funnel. All the extracts were separately concentrated to dry mass using rotary evaporator at 40°C under reduced pressure. The dry mass of DCM (~20.6 g) extract was used for phytochemical investigation. The DCM extract of M. alba was subjected to column chromatography then three fractions (F1 to F3). Fraction F2 was subjected to sub column for purification and five fractions (J1 to J5) were obtained. White amorphous solid fraction J1 yielded and the fraction J4 crystalline white solid was obtained. The solid crystals obtained from the fractions J1

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and J4 were further purified by pipette column and marked as A and B respectively. From UV, IR, 1H and 13C NMR studies, we obtained two compounds assigned as 3 methylnon-‐8-‐en-‐2one and β-‐sitosterol.

37. Tauhidur Rahman Tomal, BS (Graduation-‐2017), Isolation of Secondary metabolites from the DCM extract of the plant Morus alba L.

Abstract

Morus alba L. is a medicinal plant and widely used in folk medicine. It is available almost all over the world. The aim of our objective is to isolate secondary metabolites from M. alba. For this purpose, plant has been collected from Modhupur, Tangia l district of Bangladesh. After cleaning and drying procedure, dried plants were grinded to powder by a grinder and the powder was stored in an air tight bottle before investigations. The powdered leaves of the plant was extracted with 80% ethanol. The ethanol extract was concentrated to dry mass using rotary evaporator below 40 °C. The dry mass of ethanol extract was dissolved in water and it was partitioned successively with hexane, ethyl acetate and finally with n-‐butanol using separatory funnel. All the extracts were separately concentrated to dry mass using rotary evaporator at 40°C under reduced pressure. The dry mass of DCM (~20.6 g) extract was used for phytochemical investigation. The DCM extract of M. alba was subjected to column chromatography then three fractions (F1 to F3). Fraction F3 was subjected to sub column for purification and ten fractions (J1 to J10) were obtained. The solid crystals obtained from the fractions J8 was further purified by pipette column. From UV, IR, 1H and 13C NMR studies the compound was assigned as 2,6-‐Dimethylhept-‐6-‐en-‐2-‐ol.

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Publications

1. Analysis of DDT and its metabolites in soil and water samples obtained in the vicinity of a closed-‐down factory in Bangladesh using various extraction methods M. N. U. Al Mahmud, Farzana Khalil, Md. Musfiqur Rahman, M. I. R. Mamun, Mohammad Shoeb, A. M. Abd El-‐Aty, Jong-‐Hyouk Park, Ho-‐Chul Shin, Nilufar Nahar, Jae-‐Han Shim Environ Monit Assess. 187: 743, 2015.

Abstract This study was conducted to monitor the spread of dichlorodiphenyltrichloroethane (DDT) and its metabolites (dichlorodiphenyldichloroethylene (DDE), dichlorodiphenyldichloro-‐ ethane (DDD)) in soil and water to regions surrounding a closed DDT factory in Bangladesh. This fulfilment was accomplished using inter-‐method and inter-‐laboratory validation studies. DDTs (DDT and its metabolites) from soil samples were extracted using microwave-‐assisted extraction (MAE), supercritical fluid extraction (SFE), and solvent extraction (SE). Inter-‐laboratory calibration was assessed by SE, and all methods were validated by intra-‐ and inter-‐day accuracy (expressed as recovery %) and precision (expressed as relative standard deviation (RSD)) in the same laboratory, at three fortified concentrations (n=4). DDTs extracted from water samples by liquid-‐liquid partitioning and all samples were analyzed by gas chromatography (GC)-‐electron capture detector (ECD) and confirmed by GC/mass spectrometry (GC/MS). Linearities expressed as determination coefficients (R2) were ≥0.995 for matrix-‐matched calibrations. The recovery rate was in the range of 72–120 and 83–110 %, with <15 % RSD in soil and water, respectively. The limit of quantification (LOQ) was 0.0165 mg kg−1 in soil and 0.132 μg L−1 in water. Greater quantities of DDTs were extracted from soil using the MAE and SE techniques than with the SFE method. Higher amounts of DDTs were discovered in the southern (2.2–936×102 mg kg−1). 2. Dissipation Pattern and Residue of Fenvalerate in Tea of Bangladesh Nilufar Nahar, Hiron Moy Sharma, Mohammad Shoeb, M Iqbal Rouf Mamun, and Mihir Lal Sarker, Dhaka University Journal of Science, 63(2): 73-‐76, 2015 (July)

Abstract

Fenvalerate, a non-‐systematic insecticide is extensively used for protection of tea leaves in Bangladesh. Excessive use of insecticides with improper pre-‐harvest intervals may cause the tea unsuitable for consumption and trade. The study was designed to determine the safe per-‐harvest interval after the application of fenvalerate on tea trees at two different doses. Fenvalerate was applied on tea plants in experimental plots at the full and half of the recommended doses (0.1 kg a.i /ha and 0.05 kg a.i /ha, respectively). Tea leaves were collected at 0 (2 h after application), 1, 3, 5, 7, 10 and 14 days after application of the insecticide and made into black tea which was infused with hot water. Both brew and brew free residue were extracted, cleaned up and analyzed by GC-‐ECD. The residue levels in the brew were 0.189, 0.033 and 0.007 mg/kg at zero, 7 and 10 days, respectively, when it was applied at half of the recommended dose. In case of the recommended dose, residue levels were 0.644 and 0.010 mg/kg at 0 and 10 day, respectively. Residues were below the maximum residue level (MRL: 0.1 mg/kg) on 5 day at half of the recommended dose and on 7 day at recommended dose. Dissipation of fenvalerate followed first order kinetics at both doses with half lives of 2.6 days in brew part and 4.6 days in brew free part. Recoveries were 6.56±0.003% and 90.6±0.033% in brew part and brew free residue part, respectively, giving a total recovery of 96.6±0.036%. LOD and LOQ were 0.002 and 0.006 mg/kg, respectively.

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3. Oxygenated Secondary Metabolites from Endophytic Fungi Isolated From Ocimum sanctum Mohammad Shoeb, Mohammad Shaheen Uddin Khandaker, and Nilufar Nahar, Journal of Basic & Applied Sciences, 11, 434-‐439, 2015.

Abstract

Ocimum sanctum Linn (Tulsi), an important medicinal plant of Bangladesh is used for antimicrobial, antiinflammatory and antiulcer activities. Eleven endophytic fungi were isolated from O. sanctum. The fungal strain, labeled as OS-‐005, was cultivated at large scale on the potato dextrose agar semi solid medium and was extracted with ethyl acetate. Repeated column chromatography of the parent extract by normal phase silica gel and one of its fraction by Sephadex LH-‐20 afforded linoleic acid, R (-‐)-‐glycerol monolinoleate, 9,10,11-‐trihydroxy-‐(12Z)-‐12-‐octadecenoic acid, ergosterol, ergosterol peroxide, 1,8-‐O-‐dimethylaverantin, coriloxin, and a new natural product named sanctumol. The structures of these compounds were elucidated unequivocally by UV, IR, MS and a series of 1D & 2D NMR analyses

4. Compositions of Rice Bran Oil in some selected Bangladeshi paddy samples. Abida Sultana, Md Ripon Hossain, M I R Mamun, Mohammad Shoeb and Nilufar Nahar, Journal of Bangladesh Chemical Society, Vol. 27 (1 & 2), 33-‐37 , 2015.

Abstract

Rice bran oil was extracted from three different varieties of paddy; traditional varieties (TV,n =10), high yielding varieties (HYV, n = 7) and scented varieties (TCV, n = 3) rice samples. Percent of oil content in the rice sample varied 1.37-‐2.70. The major composition of the rice bran oils was found to be oleic (35.55-‐44.33 %), linoleic (34.17-‐42.45 %) and palmitic acids (17.66-‐19.89 %) along with small amount of stearic (1.19-‐1.50 %), arachidic (0.46-‐0.68 %), behenic (0.18-‐0.31 %) and myristic (0.20-‐0.29 %) acids.

5. Organochlorine Pesticide Residues in Poultry Meats of Bangladesh Mohammad Shoeb, Afsana Mahim, M. I. R. Mamun, Nilufar Nahar, Croatian Journal of Food Science and Technology, 8 (1) 30-‐33, (2016).

Abstract Forty poultry meat samples from four large companies were analyzed for the presence of organochlorine pesticides. Homogenized samples were mixed with silica sand and anhydrous sodium sulfate, and extracted with ethyl acetate followed by n-‐hexane: MTBE. Clean up of samples were done by silica sulphuric acid and analyzed by GC-‐ECD, and confirmed by GC-‐MS. The limit of detection (LOD) and limit of quantification (LOQ) were 0.5, 0.5 and 0.7, and 2.5, 2.5 and 3.5 ng/g, respectively. The calibration curves were linear and the correlation coefficients (r2) were 0.991, 0.992 and 0.997 for DDE, DDD and DDT, respectively and the recoveries for them were 76%, 78% and 80%. DDT and its metabolites, DDE and DDD were detected in 28 (70%), out of 40 samples and ranged 0.039-‐0.769 mg/kg.

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6. DDT and Its Metabolites in Fresh Water Fish Samples, Md. Amjad Hossain, Mohammad Shoeb and Nilufar Nahar, Journal of Food Science and Engineering, 6, 344-‐350, (2016)

Abstract

Twenty two fresh water fish samples namely Puntius sarana (Shawrputi), Cyprinus carpio (Karp), Oreochromis niloticos (Telapia), Channa punctatus (Taki), Wallogonia attu (Boal), Eutropiichthys vacha (Bacha), Macrognathus aculiatus (Baim), Ailia coila (Kajoli), Mystus cavasisus(Gulsa), Ompok pabda (Pabdha), Corica soborna (Kachki), Mystus vittatus (Tengra), Glossogobius giuris (Baila), Macrobrachium malcolmsli (Chingri), Amblypharyngodon microlepis (Mola), Anabas testudineus (Koi), Macrognathus aculiatus (Baim), Channa striatus (Shole), Heteropnueste fossilis (Shing), Puntius sophore (Small Puti) and Pseudambassis ranga (Telapia) were collected from two rivers and one cultured fish pond. The samples were extracted by QuEChERS method, cleaned up with conc. H2SO4 treatment and cleaned extracts were analyzed by GC-‐ECD. Small size cultured rui fish sample which did show detectable amount of DDT and its metabolites was used for the recovery experiments. Percent recovery was found to be in the range of 70%-‐113%. Amount of total DDTs were found to be 54.34, 48.81, 62.09, 54.72,78.81, 60.07, 47.0, 42.7, 26.31, 10.36, 25.32, 12.96, 20.10, 12.78, 17.65, and 4.71, 8.58, 11.3 and 19.01 ng/g in gulsa, pabhda, baila, bacha, baim, small puti, tengra, chanda, kachki, boal, taki, chingri, mola, shole, shing, koi, swarputi, karp and telapia fish samples, respectively. However, the residual amounts of DDTs in all the fish samples were below maximum residue limit (MRL of DDTs in fish 5.0 mg/kg) according to Codex Alimentarius Commission. The ratios of 4,4´-‐DDT/DDTs were in the range of 0.03-‐0.44 which indicated that exposure to DDT is not due to recent use.

7. Physico-‐Chemical Analysis and Composition of Camel Milk of Bangladesh, Nilufar Nahar, Waliul Islam Khan and Mohammad Shoeb, Journal of Basic & Applied Sciences, 12, 231-‐235, 2016

Abstract

Camel farming is increasing in Bangladesh but the nutritious value of the produced milk has not been studied in this geological location. The milk was coagulated by citric acid and the coagulated solid i.e. the casein (7%) and pure serum (14%) were obtained. Fat content was determined by extracting casein and the aqueous serum, separately with n-‐hexane and found 2.59% and 5.79%, respectively. The fatty acids in the fat from casein and serum were made into their methyl ester by saponification followed by esterification and analyzed by GC-‐FID. Palmitoleic, oleic and linoleic acids were predominant fatty acids found in the analyzed samples while stearic, arachidic, behenic and myristic acids were present as minor acids. Water, ash, nitrogen and lactose contents in the milk were 84%, 0.88%, 1.62% and 9.32%, respectively. The presence of vitamin B1, B2 and B6 were estimated by UV-‐VIS spectrophotometer and found 388, 64 and 116 ppm, respectively.

8. Study of Preservatives and Stimulants in Commercial Soft Drinks Abida Sultana, Robiul Islam, Md. Mazharul Islam, Mohammad Shoeb and Nilufar Nahar, Bangladesh Pharmaceutical Journal 19 (1): 68-‐74, 2016

Abstract

Commercially available soft drinks i.e., Sprite and 7up (lemon) samples of 10 different batches were studied by UV-‐Vis spectrophotometry to find out the presence of preservative and stimulant. The wavelength of absorption maxima (λmax) was 224 nm for sodium benzoate and 272 nm for caffeine. The soft drink samples were degassed, extracted with HPLC grade water and cleaned up to study sodium benzoate and caffeine content. The average quantity of caffeine was in the range of 22 to 30 μg/ml in Sprite and 20 to 30 μg/ml in 7up (lemon), whereas the average quantity of sodium

29

benzoate was in the range of 181 to 191 μg/ml in Sprite and 140 to 160 μg/ml in 7up (lemon) samples. The correlation coefficients of the calibration curves of sodium benzoate and caffeine were found to be 0.9972 and 0.9862, respectively. Recovery experiment was done by spiking sodium benzoate at 20 μg/ml level with 10 replicate studies. The mean recovery of sodium benzoate was 89.14 ± 2.14 %. The reproducibility and repeatability of the method was very satisfactory with low value of RSD. The present method can be successfully applied for the study of sodium benzoate and caffeine in commercial soft drinks.

9. Determination of the Toxicity Level of Imidacloprid in Rice sample Produced in Bangladesh Z. S. Munia, M. Shoeb, M. Kamruzzaman, M. I. R. Mamun and N. Nahar, Journal of Bangladesh Chemical Society, Vol. 28 (1 & 2), 1-‐4, 2016.

Abstract

Imidacloprid, a systemic chloro-‐nicotinyl insecticide is used to control sucking insects in stored food grains. It acts on several types of post-‐synaptic nicotinic acetylcholine receptors in the nervous system followed by failure of the neuron to propagate any signal. Relative to human health, exposure to high doses may be associated with degenerative changes in the testes, thymus, bone marrow and pancreas. The primary effects of longer term, lower-‐dose exposure to imidacloprid are on the liver, thyroid, and body weight (reduction). Low-‐ to mid-‐dose oral exposures have been associated with reproductive toxicity. The current study presents an improved method for analyzing the toxicity of imidacloprid in rice samples which we consume everyday. The toxic effect in rice was evaluated against adults Sitophilusoryzae. The percentage of insect mortality at 24, 48 and 72 hours after treatment were 30%, 54%, 61% and 74% at 1.25, 2.5, 5 and 10 µg g-‐1 respectively indicated that imidacloprid possessed the highest toxic (74%) effect at 10.0 µg g-‐1 level. Mortality percentage was found directly proportional to the level of concentrations and the time after treatment.

10. An easy and cheaper method to assay paracetamol and metronidazole, Abida Sultana, Md. Ahasanul Hoque Sakib, Md. Golam Kibria, Md. Robiul Islam, Mohammad Shoeb and Nilufar Nahar, Journal of Bangladesh Chemical Society, Vol. 28(1 & 2), 1-‐11, 2016

Abstract

An easy and cheaper method was developed to assay paracetamol and metronidazole in pharmaceutical products by UV-‐Vis spectrophotometry. The selectivity was good with their overlain spectra (λmaxat 243 and 310 nm), linearity was excellent with correlation of coefficients (r2) of 0.9993 and 0.9996, limit of detection were 0.05 and 1 µg/mL and recoveries were 99.88 ± 0.04 to 109.19 ± 0.03 and 99.66 ± 0.11 to 103.59 ± 0.09 % for paracetamoland metronidazole, respectively. The amount of paracetamol in 500 mg tablets were found to be in the range of 401.01 ± 1.75 to 553.50 ± 2.50 mg whereas 400 mg of metronidazole tablets in the range of 404.12 ± 3.38 to 497.18 ± 2.39 mg.

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11. A new steroidal glycoside from Corypha taliera Roxb. a globally endangered species M. Shoeb, M. Khondker, and N. Nahar , Nat Prod Res, 30(3): 281-‐5, 2016

Abstract

A new steroidal glycoside, β-‐sitosterol-‐3-‐O-‐a-‐L-‐rhamnopyranosyl-‐(1→4)-‐β-‐Dxylopyranosyl-‐(1→4)-‐b-‐D-‐glucopyranosyl-‐(1→4)-‐b-‐D-‐glucopyranoside was isolated from the methanol extract of the pericarp of Corypha taliera Roxb. The reversed-‐phased HPLC analysis of the methanol extract of the pericarp of C. taliera Roxb. (Talipalm), a rare species of Arecaceae family, afforded a new steroidal glycoside, b-‐sitosterol-‐3-‐O-‐α-‐L-‐rhamnopyranosyl-‐(1→4)-‐βD-‐xylopyranosyl-‐( 1→4)-‐β-‐D-‐glucopyranosyl-‐(1→4)-‐b-‐D-‐glucopyranoside (1). The structure of the compound was elucidated unequivocally by UV, IR, HR-‐ESI-‐MS, 1H and 13C NMR spectroscopic studies.

12. Evaluation of Vitamin B1, B2 and B6 Tablets in Bangladesh by UV-‐Vis Spectrophotometer, Abida Sultana, Md. Sharear Saon, Mohammad Shoeb and Nilufar Nahar (2016). Indian Journal of Pharmaceutical and Biological Research (IJPBR), 4(4):32-‐38

ABSTRACT Thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) were assayed in pharmaceutical dosages form. Thiamine hydrochloride of 100 mg dose of six different companies, riboflavin of 5 mg dose of five different companies and pyridoxine hydrochloride 20 and 25 mg dosages of two different companies of Bangladesh were extracted from the tablets, cleaned up and their active ingredients were evaluated by UV-‐Vis spectrophotometer at 432, 445 and 292 nm, respectively. Evaluation was carried out with respect to calibration curves of their standard reference samples. Amount of thiamine hydrochloride in 100 mg tablets of six different companies were found to be in the range of 37.62±1.11 mg to 79.03±0.42 mg. For riboflavin in 5 mg tablets of five different companies, active ingredient was found in the range of 6.78±0.19 mg to 8.23±0.15 mg. In case of pyridoxine hydrochloride tablets, it was found that 20 mg tablet of one company contained 21.75±0.41 mg and 25 mg tablet of another company contained 29.72±0.59 mg of active ingredient. Recovery experiments were done by spiking excipients of the respective medicines at three different concentration levels with 5 replicate studies. Correlation coefficients (r2) were found to be 0.99 for three vitamins and recoveries were 82.26± 0.10, 99.61± 0.06 and 109.91±0.12 for thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2) and pyridoxine hydrochloride (vitamin B6) respectively.

13. Hypoglycemic activity of Scoparia dulcis L. extract and isolation of flavonoid glycosides, Khan S H, Matin M, Sultana N, Moshiuzzaman M, Ali, L Rokeya, B, Khan A K A, Shoeb M and Nahar N (2016), J. Bang. Chem. Soc, 28 (1&2) 1-‐4

Abstract Methanol extract of Scoparia dulcis Linn was tested on normal, Type 1 and Type 2 diabetic Long Evans rat model rats. The extract showed significant hypoglycaemic effect on Type 1 model rats at fasting (control 28 ± 5.34 vs 23.57 ± 2.82 ; 60 min time duration ), simultaneously with glucose 36.29± 4.52 vs 32.58 ± 6.79 ; 75 min time duration) and 30 min before (17.06 ± 3.56 vs 10.97 ± 5.58; cumulative effect) glucose load. From the hypoglycaemic extract six flavonoid glycosides; quercetin-‐3-‐O-‐β-‐D-‐glucopyranosyl-‐(1→2)-‐β-‐D-‐glucopyranoside (I), quercetin-‐3-‐O-‐β-‐D-‐xylopyranosyl-‐(1→2)-‐β-‐D-‐glucopyranoside (II), quercetin-‐3-‐O-‐α-‐L-‐rhamnopyranosyl-‐(1→6)-‐β-‐D-‐galactopyranoside (III), quercetin-‐3-‐O-‐rutinoside (IV), kaempferol-‐3-‐O-‐rutinoside (V) and isorhamnetine-‐3-‐O-‐rutinoside (VI) were isolated and characterized by UV, IR and 1 & 2D NMR spectroscopy. Compound II, III, V and VI were tested on four bacteria namely, Bacillus subtilis, Streptococcus pyogenes, Proteus mirabilis and

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Klebsiella pneumonia. All the four compounds showed moderate antibacterial effect against Streptococcus pyogenes.

14. Evaluation of Diclofenac by UV-‐Vis Spectrophotometer in Some Locally Produced Tablets, M K Hasan, M A Hossain, A Sultana, M Shoeb and N Nahar, Dhaka Univ. J. Sci. 65(2): 163-‐165, 2017 (July)

Abstract Diclofenac is a common and randomly used pharmaceutical product, was evaluated for its active ingredient by UV-‐Vis spectrophotometer at 282 nm. Diclofenac sodium of 50 mg dose of seven different companies of Bangladesh was extracted from the tablets, cleaned up and the active ingredients was evaluated. Evalution was carried out with respect to calibration curve of standard diclofenac sodium. Amount of diclofenac in 50 mg tablets of seven different companies were found to be in the range of 47.91 ± 0.90 to 58.52 ± 0.41 mg. Recovery experiments were done by spiking excipient of the medicine at two different concentration levels with 5-‐7 replicate studies. Correlation coefficient (r2) was found to be 0.9974 and the recovery was 103.39 ± 3.93 to 107.96 ± 3.56 % for the drug

15. Chemical and biological activity of mushrooms naturally occurring in Bangladesh, Mohammad

Shoeb, Prithu Mondal, Henrik Kylin and Nilufar Nahar (2017), Asian Journal of Pharmacognosy, 1(3):42-‐51

Abstract Five freeze-‐dried mushroom species namely Agaricus bisporus, Coprinus micaceus, Pleurotus. ostreatus, Termitomyces clypeatus and T. heimii were extracted separately with dichloromethane:methanol followed by partitioned using hexane, dichloromethane and methanol. Silica gel column chromatography of hexane extract of T. heimii and dichloromethane extract of P. ostreatus afforded 2,4-‐di-‐hydroxy-‐9-‐octadecenoic acid and 2-‐hydroxy-‐9-‐octadecenoic acid, respectively. The nhexane extracts of mushrooms were analyzed for the presence of n-‐alkanes and fatty acid compositions. n-‐Alkanes were found in T. heimii only. Different extracts of mushrooms were evaluated for antimicrobial activity against a wide range of Gram-‐positive and Gram-‐negative bacteria and fungi, general toxicity and antioxidant activity. 16. Residual DDTs and Fatty Acid Compositions in Popular Two Sea Fish Samples, Mohammad

Shoeb, Muhammad Shamim Al Mamun1, Radwan Ebna Noor, Md. Nashir Uddin AL Mahmud, M. I. R. Mamun and Nilufar Nahar, Dhaka Univ. J. Sci. 65(1) 77-‐80, 2017

Abstract

Fatty acid compositions, DDT and its metabolites of popular two sea fishes i.e., Pampus argenteus (Pomfret) and Lates calcarifer (Vernacular) were determined. Lipid was extracted by ethylacetate, saponified and converted to methyl ester using BF3-‐MeOH to determine fatty acid compositions by GC-‐FID.For DDTs,edible parts of the fish samples were extracted by solid phase dispersion method, cleaned up with concentrated H2SO4 and finally analyzed by GC-‐ECD. The LOD and LOQ for DDE, DDD, 2, 4´-‐DDT and 4, 4´-‐DDT were found to be 0.39, 0.39, 1.56, 1.56 and 1.36, 1.36, 4.89 and 4.89 μg/kg respectively. Internal Standard CB53 (0.25mg/g) was used to determine the recovery of the experiment and was within range of 77.48-‐92.35 %. The residual amount of DDT (2, 4´-‐DDT+4, 4´-‐

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DDT), DDE and DDD in pomfret and vernacular were found to be in the range of 13.56-‐81.05, 3.86-‐21.08, 4.32-‐35.4, and 23.44-‐85.59, 2.90-‐5.81 and 6.33-‐12.44 μg/kg respectively. The ratios of DDT/ΣDDTs in pomfret and vernacular were 0.675 and 0.773 respectively. Among the saturated fatty acids, palmitic acid was predominant (62.84% for pomfret and 41.81% for vernacular) whereas among the unsaturated fatty acids oleic acidwas predominate and higher in pomfret (15.11 %). 17. Bioaccumulation of Mercury in Fish Species from Different Trophic Level Mohammad Shoeb,

Md. Amjad Hossain, Md. Golam Kibria, Tonima Mustafa and Nilufar Nahar, Journal of Food Science and Engineering 7, 363-‐367, 2017

Abstract

Mercury (Hg) is an environmental pollutant, and can bioaccumulate and biomagnify in the food web. Twenty four fish samples of different tropic level i.e., Herbivore, Omnivore and Carnivore were collected from two contaminated rivers, the Megna and the Burganga which surround the Dhaka city for the assessment of toxic Hg. Fourteen samples were found to contain Hg in the range of 0.01-‐0.09 mg/kg. The highest Hg (0.09 mg/kg) was found in the Carnivore, Bele (Glossogobius giuris). However, all fish species had lower amount of Hg than the maximum Hg limit (0.5 mg/kg for fish) set by WHO. Among all the fish species, the order of bioaccumulation were carnivore> omnivore> herbivore. Kajoli (Ailia coila), Shing (Heteropnuestes fossilis), Rui (Labeo rohita), Chao (Tryauchen vagine), Rita (Rita rita), Hilsha (Tenualosa ilisha), Small Puti (Puntius sophore), Bacha (Eutropiichthys vacha) and Chingri (small prawn) were not found to contain any Hg which indicated that fish species from these rivers are safe for human consumption.

Enclosure 7 Others Manuscript A total of 20 papers have been published in peer reviewed journals and reported in the form. We were also working hard to complete HEQEP project from the UGC within time. Moreover, PhD students were working hard for writing and submitting theses. However, we are concentrating publications of more papers now. The following manuscript are in line for submission/preparation/under review process.

1. Nilufar Nahar, Mohammad Shoeb, Salma Akter Mou, Rafiza Islam, Sulfur Drugs in Poultry and Beef Samples of Bangladesh, Food Control, 2017 (ready to submit).

2. Al Mahmud, Md. Nashir Uddin, Nahar Nilufar, Mohammad Shoeb, Mamun, M Iqbal Rouf; Sutra Dhar, Sepra Rani, Distribution and dissipation of diazinon and chlorpyrifos in the paddy cultivation system of Bangladesh, Journal of Agricultural and Food Chemistry, 2017 (under review).

3. Zerin Sultana Munia, Mohammad Shoeb, M. I. R. Mamun1, Nilufar Nahar, Dissipation Pattern of Quinalphos in Cauliflower, Tomato and Bean, Journal of Consumer Protection and Food Safety, 2017 (under review).

4. Md. Ahasan Ul Hoque Sakib, Rehnuma Tabassum, Mohammad Shoeb, Robiul Islam, Nilufar Nahar, Investigation of three surfactants in surface water by spectrophotometric method, 2017 (Manuscript final).

5. Mohammad Shoeb, M, Khan, AH, Jeouty, JN, Rehenuma Tabassum, Nilufar Nahar, Emerging Contaminants in Marine Environment of the Bay of Bengal, 2017 (Manuscript preparation).

6. Md. Nashir Uddin Al Mahmud, Mohammad Shoeb, M. I. R. Mamun, Mohammad Moniruzzaman, Niamat-‐E Khuda, Nilufar Nahar, Analysis of some pesticides in some local vegetables of Bangladesh, Food Security, 2017 (under review).

7. Abida Sultana, Mohammad Shoeb, Iqbal Rouf Mamun and Nilufar Nahar. Analysis of Aflatoxins; B1, B2, G1 and G2 in Some Rice Samples of Bangladesh. World Mycotoxin Journal (Manuscript final).

8. Abida Sultana, Mohammad Shoeb, Iqbal Rouf Mamun and Nilufar Nahar. Carbofuran Residues in Commercial Turmeric Powder. Journal of the Science of Food and Agriculture 2017 (Manuscript final).

9. Abida Sultana, Mohammad Shoeb, Iqbal Rouf Mamun and Nilufar Nahar. Studies of Dissipation Patterns of Diazinon in Bean, Cauliflower, Eggplant and Tomato 2017 (Manuscript preparation).

10. Abida Sultana, Mohammad Shoeb, Iqbal Rouf Mamun and Nilufar Nahar. Post-‐Harvest Intervals of Carbosulfan in Tomato 2017 (Manuscript preparation).

11. Abida Sultana, Mohammad Shoeb, Iqbal Rouf Mamun and Nilufar Nahar. Analysis of Pesticide Residues in Four Different Vegetable Samples from Market 2017 (Manuscript preparation).

12. Tonima Mustafa, Mohammad Shoeb, M. N. Naser, Gulshan Ara Latifa and Nilufar Nahar. Organohalogen pesticide residues of fishes from different trophic levels of Meghna River, Bangladesh. Bul. Environ. Contam. Toxicol. 2017 (Manuscript final)

13. Tonima Mustafa, Mohammad Shoeb, M. N. Naser, Gulshan Ara Latifa and Nilufar Nahar. Organochlorine pesticide residues of selected fishes of Buriganga River, Bangladesh. Environ. Contam. Toxicol. 2017 (Manuscript preparation)

14. Tonima Mustafa, Mohammad Shoeb, M. N. Naser, Gulshan Ara Latifa and Nilufar Nahar. Human health Risk assessment of DDT residues in fishes collected from Meghna River, Bangladesh, Springer Plus. 2017

15. Zerin Sultana Munia, Mohammad Shoeb, M. I. R. Mamun, Nilufar Nahar, Identification and Quantification of Imidacloprid in Rice and Wheat Flour in Bangladesh, 2017 (will be revised for submission to another journal).

16. Farzana Khalil, Nilufar Nahar, Mohammad Shoeb and M. I. R. Mamun. DDTs in fish samples of Chittagong Chemical Complex area in Bangladesh (Manuscript).

17. Farzana Khalil, Nilufar Nahar, Mohammad Shoeb and M. I. R. Mamun. DDTs in human blood of former DDT factory in Bangladesh: Chittagong Chemical Complex area. (Manuscript).

18. Farzana Khalil, Nilufar Nahar, Mohammad Shoeb and M. I. R. Mamun. Dissipation pattern of cypermethrin in five different vegetable samples of Bangladesh. (Manuscript).

19. Farzana Khalil, Mohammad Shoeb, Nilufar Nahar, M. I. R. Mamun, Analysis of pesticide residues in summer vegetables of Bangladesh. (Manuscript).

Documents

Grant Application BAN04 · 2019-09-04 · Deadline$2017$–$see$isp.uu.se/documents$ $ 5$(31)$ $ credits.$Ph.D$and$M.Phil.$studentsgive$oral$presentation$of$their$research$workin$front$of$faculty