GS Duc - Saponin DL Chua Saponin

8/13/2019 GS Duc - Saponin DL Chua Saponin

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Panax notoginseng (Burk.) F.H. Chen;Ho Ngu Gia B (Araliaceae)

TAM THATNhan sam Tam that,Kim bat hoanRe cu a che bien cua

cay TAM THAT

Trc y Tam tht c xc nh l Panax ps eudoginseng,nhng sau c ngh l P. no tog ins eng[Nguyn Minh c, Nguyn Thi Nhm v on T Tuyt Trinh,Tp ch Dc liu, Tp 4, s 3, trang 68 (1999).]


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- Cay, la tng t Nhan sam, nhng la kep co 5-7 la chet.- Gan la chnh co gai, hoa xanh nhat.- Cum hoa: tan n- Qua mong hnh cau, chn o, hat hnh cau, - -Cu u nan + soc doc.Nguon goc, trong trot : nguon goc Van nam ,Trung quoc. Trc ay co trong mot so tnhbien gii pha Bac nh Lao cai, Cao bang, Hagiang nhng hien nay viec trong trot khong phattrien.

I- AC IEM THC VAT


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Tam thatPanax notoginseng(Burk.) F.H. Chen;Ho Ngu Gia B(Araliaceae)

I- AC IEM THC VAT


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Tam thatPanax notoginseng(Burk.) F.H. Chen;Ho Ngu Gia B (Araliaceae)

I- AC IEM THC VATRe cu Tam that


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Re cu a phi say kho hoac che bien cua cay tamthat,Che bien: La cu rieng, re con rieng. Phi nang heocu roi lan, vo, lam nhieu lan cho en khi cu chac lai,phi kho roi xoc vi sap, trong bao cho vo ngoaibong ep. Khi dung th hap mem, thai phien, co thetan bot e dung dan di dang tra, dang ru thuochoac nau chung vi ga Phan khong tao phien cth say kho, xay bot.Dc lieu sau khi che bien co hnh dang thay oi,hnh con quay (con vu) hay hnh cu ca rot dai 2-6 cm.Mat ngoai nau xam hoac vang xam, co nhieu vet seocon laicua re nhanh. The chat cng chac, v luc auhi ang, sau hi ngot.

II- BO PHAN DUNG VA CHE BIEN


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Re cu chu yeu la cac saponin dammaran co cau tao:- 20(S)-Protopanaxadiol: chu yeu G-Rb1 (1,8 ), G-Rb2, G-Rd, N-R4, N-Fa, Gypenosid-XVII . . .- 20(S)-Protopanaxatriol: chu yeu G-Rg1 (1,9 ), G-Re, G-Rg2, G-Rh1, N-R1, N-R2, N-R3, N-R6 . . .N= notoginsenosidCac bo phan khac cua cay nh re con, la hoa eu cocha saponin dammaran

Saponin: ch gom nhom dammaran.III- THANH PHAN HOA HOC


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1. Soi vi phau va soi botIV. KIEM NGHIEM1. Bn

2. M mm v

3. ng tit trong vng m mm v4. ng tit trn b libe

5. Tia ty

6. Libe

7. Tng pht sinh libe-g8. Mch g

9. M mm ty

Vi phu


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1. Soi vi phau va soi botIV. KIEM NGHIEM1. Mnh bn & m mm

2. M mm v cha tinh bt

3. Mnh mch

4. Tinh bt

Bt

2

1

3

34


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2. Phn ng mu- Phan ng Liebermann Burchardt: can dch chietMeOH/Ac2O + H2SO4 vang o tm xanh luc.- Dch chiet MeOH/UV 365 nm huynh quangxanh nhat (+ acid boric/aceton + acid citric) huynh quang vang luc di UV 365 nm.3. SKLM+ Chat hap phu: silica gel F 254+ Dch cham: can saponin/EtOH, dungmoiCHCl3:MeOH:H2O (65:35:10, lp di),+ Phat hien bang UV 254, H2SO4 20 /con 50 .+ So sanh vi G-Rb1 va G-Rg1 chuan .4. HPLC

IV KIEM NGHIEM


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- Theo ong y, tam that la thuoc tr huyet, codung cam mau, giam viem, giam au.- Dung cha: ho ra mau, non ra mau, chay maucam, ai tien ra mau, t cung xuat huyet, chanthng.- La v thuoc bo nh nhan sam, ac biet tot chophu n sau khi sinh n (ham ga . . .).- Phu n co thai khong nen dung.- Lieu: 6-12 g/ngay.

V. TAC DUNG VA CONG DUNG


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Panax vietnamensis Ha et Grushv.;Ho Ngu Gia B (Araliaceae)

SAM VIET NAMSam Ngoc Linh, Sam K5,Sam ot truc, Cu Ngai rm con

Than re va re cu a che bien cuacay Sam Viet namRhizoma et Radix Panacis vietnamensis)


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I- VAI DONG LCH S Sam Viet nam, con goi SamNgoc linh, Sam K5, la loaiPanax moc hoang da, cphat hien tai nh Ngoc Linhthuoc tnh Gia lai-Kon tumvao nam 1973. ay la loaiPanax au tien c tmthay trong vung thuoc khhau nhiet i va gan xchao nhat.Theo ieu tra, ay la caythuoc dau cua dan tocngi Se-ang song trenday Trng sn, dung etang lc va cha nhieubenh tat.


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I- VAI DONG LCH S

Sam Viet nam(Panax vietnamesis Ha etGrushv. Araliaceae)


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I- VAI DONG LCH S

Nhieu cong trnh nghien cu a e ngh cac ten khoahoc khac nhau, nhng sau cung Sam VN c xacnhan va cong bo la mot loai Panax mi cua the gii viten la: Panax vietnamensis Ha et Grushv. Ho Ngu giab (Araliaceace)


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II- AC IEM THC VAT- Cay, la, hoa tng t cac cay thuoc chi Panax.-iem khac biet so vi Nhan sam:+ Qua co om en.+ a so (85 ) ch co 1 hat (Nhan sam: 2 hat),ch co mot t qua 2 hoac oi khi 3 hat.+ Bo phan di mat at cua cay sam moc hoanggom mot than re rat phat trien, gom nhieu otnen c goi la Sam ot truc va tan cungbang mot re cu nho.Tuy nhien, cay Sam VN trong co khuynh hngcho bo phan di at la cac re cu, thanh tngchum.


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II- AC IEM THC VAT

Vung nui Tra Linh, nh Ngoc Linh, tnh Quang Namni co vung trong Sam VN t nhien


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II- AC IEM THC VATCay Sam Viet nam trongdi tan rng t nhien


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II- AC IEM THC VATTan qua Sam Viet nam


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II- AC IEM THC VATQua ac trng cua Sam Viet nam vi cham en au va a so (85 ) ch co 1 hat.


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II- AC IEM THC VATBo phan di mat at cua Sam Viet nam


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II- AC IEM THC VAT

Cay Sam Viet nam trong


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II- AC IEM THC VAT

Bo phan di at Sam Viet nam trong 2-6 tuoi


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1. Re va than re cha ham lng saponin rat cao(15-20 ) chu yeu thuoc nhom dammaran, ch rat tsaponin nhom oleanIII. THANH PHAN HOA HOC

T bo phan di mat at cua Sam VN, Nguyen Minhc va CS. a chiet xuat phan lap va xac nh hn50 saponin, trong o co 24 saponin dammaran miat ten vina-ginsenosid-R1-24.1. N. M. Duc et al.,Chem. Pharm. Bull., 41, 2010 (1993);2. N. M. Duc et al., Chem . Pharm. Bull., 42, 115 (1994);3. N. M. Duc et al., Chem . Pharm Bull., 42, 634 (1994);4. N. M. Duc et al. Saponins Used in Modern and TraditionalMedicine Plenum Press NewYork and London pp. 129-149(1996).


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1) Re va than re:III. THANH PHAN HOA HOCCac saponin a biet gom:- Nhom protopanaxadiol:G-Rb1 (2.0 ),G-Rb2, G-Rb3 (0.1 ), G-Rd (0.89 ),pseudo-G-Rc1, gypenosid-IX, gypenosid-XVII,quinquenosid-R1, noto-ginsenosid-Fa, vamajorosid-F1.- Nhom protopanaxatriol:G-Re (0.17 ), 20-gluco-G-Rf, G-Rg1 (1.37 ), G-Rh1, 20R)-G-Rh1, pseudo-G-Rs1, notoginsenosid-R1; (0,36 ), va notoginsenosid-R6.


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III- THANH PHAN HOA HOC

OH

O-Glc2-Xyl

HO

O

OH

- Cac Ocotillol saponin: pseudo-G-RT4, 24(S)-pseudo-G-F11, majonosid-R1 (0.14 ), vamajonosid-R2 (5.3 ), saponin chu yeu cua SamVN.

- Saponin co aglycon la acid oleanolic: G-Ro andhemslosid-Ma3.

Majonosid-R2


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III- THANH PHAN HOA HOCCac saponin miNhom ocotillol

OH

O-R1

HO

O

OHR2 R1 R2

V-R1 -Glc2-Rha -CH36 AcV-R2 -Glc2-Xyl -CH36 AcV-R5 -Glc2-Xyl4-Glc -CH3

V-R6 -Glc2-Xyl -CH36 GlcV-R14 -Glc2-Xyl -CH2OH


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III- THANH PHAN HOA HOCCac saponin miNhom ppd va ppt R3-OOHR1-O

R1 R2 R3V-R4 -Glc2-Glc -OH -GlcV-R7 -Glc2-Glc2-Xyl -H -GlcV-R16 -Glc2-Xyl -H -GlcV-R17 -Glc2-Glc -H -AraV-R18 -Glc2-Glc -H -Xyl

R2


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III- THANH PHAN HOA HOCCac saponin miNhom mach nhanh bien oiGlc-O

OH

R1-O

R1 R2 R3V-R8 -Glc2-Glc -OH (A)V-R15 -H -O-Glc (A)V-R19 -Glc2-Glc -H (B)V-R9 -Glc2-Glc -H (C)V-R12 -H -O-Glc (D)V-R13 -Glc2-Glc -H (E)VR-21 24S -H -O-Glc (E)VR-22 24R -H -O-Glc (E)

R2

R3

OHGlc-O O2HGlc-OGlc-O OHGlc-O OH

HO

(A)

(B)

(C)

(D)

(E)

HO

HO


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II- THANH PHAN HOA HOC

RV-R10 -GlcV-R11 -Glc2-Xyl

Glc-O

Glc2-Glc-O

HO

HO OR

O

OH

V-R3


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III- THANH PHAN HOA HOCGlc-O

Glc2-Glc-O V-R20

HOO

Glc-O

Glc2-Glc-O V-R23

HO

OHGlc-O

V-R24

HO

Glc2-Glc-OCH2OH


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Cac saponin a biet:Nhom protopanaxadiol: G-Rb2, G-Rb3, G-Rd, G-F2,notoginsenosid-Fe, notoginsenosid-Fc, gypenosid-IX.Nhom protopanaxatriol: G-Re, G-Rg1, pseudo-G-Rs1.Cac saponin occotilol: 24 (S)- pseudo-G-F11, VG-R1.Vo Duy Huan, Nguyen Minh Duc, R. Kasai and K. Yamasaki,P r o c e e d i n g o f t h e T h i r d I n d o c h i n a C o n f e r e n c e o n P h a r m a c e u t i c a l

Sciences, 20-23 May, 2003, Bangkok, Thailand, pp. OP65-OP75.

2. T la, Vo Duy Huan, Nguyen Minh c va CS achiet xuat, phan lap va xac nh cau truc cua 19saponin, trong o co 8 saponin dammaran mi cat ten la vina-ginsenosid-L1-L8.

III- THANH PHAN HOA HOC


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III- THANH PHAN HOA HOCR2-OOH

R1-OR1 R2

20(S)PPD -Glc2-Glc -OHV-R7 -Glc2-Glc2-Xyl -GlcV-L1 -Glc2-Glc2-Xyl -HV-L2 -Glc2-Glc2-Xyl -Glc6-Ara(f)

Saponin dammaran mi trong la


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III- THANH PHAN HOA HOCSaponin dammaran mi trong laR2-OOH

R1-OR1 R2

V-L3 -Glc2-Glc -Glc6-XylV-L4 -Glc2-Glc2-Xyl -Glc6-XylV-L8 -Glc2-Glc -Glc

OH


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III- THANH PHAN HOA HOCSaponin dammaran mi trong laR2-OOH

R1-OR1 R2

V-L3 -Glc2-Glc -Glc6-XylV-L4 -Glc2-Glc2-Xyl -Glc6-XylV-L8 -Glc2-Glc -Glc

OH


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R2-OOH

R1-O

HO

R1 R2V-L5 -Glc -Glc -Ara(f)V-L6 -Glc -Glc6-XylV-L7 -Glc2-Glc -Glc6-Xyl

Saponin dammaran mi trong laIII- THANH PHAN HOA HOC


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Saponin dammaran mi trong laIII. THANH PHAN HOA HOC

HO

HO OR

O

OH24(S)

R20(S),25 epoxydammaran -H-3,6,12, 24(S) tetrolV-L8 -Glc2-Rha


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Cc thnh phn khc:

- Tinh du: 0.05 - 0.01 trong thn r ti.- Glucid: 6.19 (dng tdo) v 26.77 (sau khithy phn)

- Acid bo: acid palmitic, acid stearic, acid oleic, acidlinoleic, acid linolenic ... Trong hm lng acidlinoleic v linolenic chim n 43 .- Amino acid: 17 amino acid gm nhiuamino acidthit yu nh Tryp, Lys, His, Arg, Threo, Ser, Pro, Gly,Ala, Val , Leu, Isoleu - Nguyn tvi lng: Fe, Sr, Ti, B, Rb, Mn, Zn, Br,Ni, Cu, Co, Cr, Se

III. THANH PHAN HOA HOC


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III. THANH PHAN HOA HOC

CH2 CH CHOH C C) CH C C CH CH)5 CH P v)H H

H

HCH2 CH CHOH C C) C C CH CH)5 CH Pv)

OA c

OHCH2 CH CHOH C C) C C CH CH)5 CH Pv)

H

HCH2 CH CH C C) CH CH CH CH)n CH Pv)

R1 R2R1: OH orOAc; R: OH orOAc; n = 6 ?)

OHCH2 CH CHOH C C) C C CH CH)5 CH Pv)

H

H


OH

H


OH

Trn Cng Lun vCS (1990) phn lp v xc nh7hp chtpolyacetylen, trong c2 hp cht mi l

Pv2 and Pv7. (falcarinol)


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1. Soi vi phau va soi bot.III. KIEM NGHIEM1. Bn2. ng tit vng m mm v3. Tia ty4. B libe5. Tng pht sinh libe-g6. Mch g7. ng tit vng ty8. M mm ty.


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1. Soi vi phau va soi bot.III. KIEM NGHIEM

1 2

3 4

5 6

1. Mnh nha mu nu2. Mnh mch3. M mm cha tinh th

Ca oxalat4. ng tit5. Mnh m mm mang

mnh nha

6. Tinh bt


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2. Cc phn ng- Phan ng Liebermann Burchardt: can dch chietMeOH/Ac2O + H2SO4 vang o tm xanh luc.- DVN IV: + Phn ng to bt.+ CnMeOH + dd SbCl

3 Hunh

quang xanh 365 nm3. SKLM+ Chat hap phu: silica gel F 254+ Dch cham: can saponin/EtOH,+ H dung moi: CHCl3:MeOH:H2O(65:35:10, lp di),+ Phat hien: H2SO4 20 /con 50 .+ So vi MR2, G-Rb1 va G-Rg1

III. KIEM NGHIEM

1.Cao MeOH TP 4. G-Rd2. Saponin TP 5. G-Rg13. G-Rb1 6. M-R2

1 2 3 4 5 6


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III KIEM NGHIEM

Sac o HPLC Sam VNtrc (tren cung) va saukhi che bien (hap 2, 4,6, 8h)

4. HPLC


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Tac ong dc lyIV- TAC DUNG VA CONG DUNGCao Sam Viet namTang lc, chong met moi, kch thch TKTW, tangtrong lng c quan sinh duc thu th nghiem,tang hong cau, hemoglobin va hematocrit, hang huyet, cai thien s co bop cua tim, tacdung adaptogen, khang khuan va long mMajonoside-R2Chong stress (N.T.T.Huong va CS;1996,1997, 1998).Chong khoi u (Konoshima va CS; 1998,1999).


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Cong dung: nh Nhan sam. Ngoai ra, co tacdung khang sinh nen c dung bao che cacche pham tr viem hong. Hien nay gia Sam Viet nam at gap nhieulan Nhan sam va cac cay tng can (> 300trieu ong/kg SmVN kh, 2012). Cay thuoc quy a vao sach o can co bienphap bao ve va ay manh trong trot.

IV- TAC DUNG VA CONG DUNG


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Schefflera octophylla (Lour.) Harms.Ho Ngu Gia B (Araliaceae)DVN III ghi loai Schefflera heptaphylla (L.) Frodin

NGU GIA B CHAN CHIMSam Nam, Cay angVo than va vo re cay Ngu gia b chan

chim (Cortex Schefflerae octophyllae)


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I- AC IEM THC VAT

Cay xanh quanh nammoc o cao 800 1.000 m.Cay cao en 10m.La kep chan vt gomchu yeu 8 la chet.Hoa: tanQua hnh cau

Ngu gia b chan chimSchefflera octophylla (Lour.)Harms. -Araliaceae)


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Tan hoa va qua

Ngu gia b chan chimSchefflera octophylla (Lour.)Harms. -Araliaceae)

I- AC IEM THC VAT


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II. BO PHAN DUNGVo than va vo re phi say kho.La cung c dungIII. THANH PHAN HOA HOC1. Vo than- Tinh dau.- Saponin triterpen 5 vong, thuoc 2 nhom olean vaursan.Maeda, Ohtani, Kasai, Nguyen Minh c, Yamasaki vaCS. (1993) a chiet xuat, phan lap va xac nh 12saponin triterpen trong o co 9 saponin cau truc mi. 12saponin triterpen nay tao thanh 6 cap ong phansaponin olean va ursan.

(Maeda et al, Phytochemistry, 37, 1137 (1994)


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III- THANH PHAN HOA HOCSaponin nhom olean

Scheffoleosid A-FR1 R2 R3 R4

A OH -OH CH2OH CH3B OH -OH CHO CH3C OH -O-Ara CH2OH CH3 CaudosidD H -OH COOH CH3E H -O-GlcA2-Gal2-Glc CH2OH CH3F OH -OH CH3 CH2OH

R2

R1

R4 R3

COO-Glc-Glc4-Rha


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III- THANH PHAN HOA HOCSaponin nhom ursan

Scheffursosid A-FR1 R2 R3 R4

A OH -OH CH2OH CH3 AsiaticosidB OH -OH CHO CH3C OH -O-Ara CH2OH CH3D H -OH COOH CH3 chat a bietE H -O-GlcA2-Gal2-Glc CH2OH CH3F OH -OH CH3 CH2OH

R2

R1

R4 R3

COO-Glc-Glc4-Rha


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III- THANH PHAN HOA HOCSaponin nhom olean & ursan 6 cap ong phan

Scheffursosid A-F Scheffoleosid A-FR1 R2 R3 R4

A OH -OH CH2OH CH3 AB OH -OH CHO CH3 BC OH -O-Ara CH2OH CH3 CD H -OH COOH CH3 DE H -O-GlcA2-Gal2-Glc CH2OH CH3 EF OH -OH CH3 CH2OH F

R2R1

R4 R3

C=OOGlc6Glc4Rha

R2R1

R4 R3

C=OOGlc6Glc4Rha


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III- THANH PHAN HOA HOC2) LaT la Ngu gia b chan chim, Tran van Sung va CS.a phan lap va xac nh cac saponin triterpen nhomlupan.

R1 R2 R3 R4

A -H -COOH -H -COOH

B -H -Glc6-Glc4-Rha -H -COOH

C -H -Glc6-Glc4-Rha -OH -COOH

D -H -Glc6-Glc4-Rha -H -CH3

E -HSO3 -Glc6

-Glc4

-Rha -H -CH3

F -Glc -Glc6-Glc4-Rha -H -CH3

G -6AcGlc -Glc6-Glc4-Rha -H -CH3


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IV. KIEM NGHIEM1) Soi vi phau va soi bot:- Manh ban va manh te bao mo mem- Nhieu te bao mo cng hnh ch nhat hoac hnhnhieu canh, mang day, ong trao oi ro.- Tinh the calci oxalat hnh hop, hnh lap phng.- Hat tinh bot (4-16 mm)2) nh tnh (DVN III)- Phan ng Fehling tua o gach.- Phan ng Liebermann-Burchardt: vongo nau.- Phan ng tao bot.- Bot dc lieu + dd ong acetat 10 mau lucsau 4 gi.- SKLM: Silicagel G; He dung moi: n-BuOH-EtOH-NH4OH (7:2:5); phat hien: TT vanilin sulfuric cacvet saponin mau tm o.


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V. TAC DUNG VA CONG DUNGY hoc co truyen dung lam thuoc thong tieu,cha phu thung, phong thap.Ngoai ra con co cong dung:- Bo, tang lc, chong met moi.- Tr tieu hoa.- Chong viem, tr au nhc, thap khp.Lieu 12-20 g/ngay


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Dioscorea tokoro MakinoHo Cu nau (Dioscoreaceae)

TY GIAIXuyen Ty giai, Phan Ty giai, Tat giaThan re cua cay Ty giai


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I- AC IEM THC VAT- Cay leo bang than quan.- Re phnh thanh cu.- La moc so le, hnh tim, gan hnh chan vt,cuong dai.- Cum hoa: bong. Hoa nho, mau xanh, n tnhkhac goc.- Qua nang co canh.Cay moc cac tnh Trung quoc giap bien giimien Bac nc ta.Nhieu loai Dioscorea khac cung co chadiosgenin va cung c dung.
http://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02692.JPG


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T gii (Dioscorea tokoro Makino)

Cy c
http://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02690.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02692.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02689.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02692.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02690.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02688.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02680.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02676.JPG


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T gii (Dioscorea toko ro Makino)Cy ci
http://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02680.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02683.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02676.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02692.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02689.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02692.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02689.JPGhttp://flowers.la.coocan.jp/Dioscoreaceae/Dioscorea%20tokoro/DSC02690.JPG


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II- BO PHAN DUNGThan re Ty giai phi say kho.III- THANH PHAN HOA HOCCha saponin steroid. Sapogenin toan phan 1-1,5 , quan trong nhat la diosgenin.

OCH3

HO

O

H

Ngoai ra, con co yonogenin, tokorogenin,kogagenin Diosgenin


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V- CONG DUNGY hoc co truyen: Ty giai c dung lam thuoc li tieu,cha viem bang quang, viem nieu ao, thap khp,phu.Lieu: 12-18 g/ngay, dang thuoc sacDung lam nguyen lieu e chiet diosgenin e tong hpthuoc steroid:- Trc khi chiet xuat, xay dc lieu, u vi nc cothem chat kch thch sinh trng nh acid gibberellic,acid 3-indol acetic e tang ham lng diosgenin.- Loai tap bang CHCl3. Chiet bang etanol. Thu hoietanol.- Thuy phan tiep bang HCl 2N (un cach thuy/5h)- Loc tua, ra tua bang NaHCO3. Say Bot kho.- Ket tinh trong cyclohexan nong tinh the.


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Costus speciosus (Koenig) SmithHo Ma do (Costaceae)

MA DOCat loi, ot ang, Se vong, Cay chotThan re cua cay Ma doBup va canh non cung c dung


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I- AC IEM THC VAT- Cay thao, song lau.- Than re to, nac, moc bo.- La moc so le, hnh trngthuon, co be.-Cum hoa: bong chuy ngon than, la bac mau o,trang mau trang, hongvang.- Qua nang hnh trng haybau duc.Cay a am, moc hoangnhieu ni tren nc ta.


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II- BO PHAN DUNGThan re, la non, canh non.III- THANH PHAN HOA HOCThan re cha:- Saponin steroid, trong o diosgenin chiem hamlng cao (1,5-2 ).Ngoai ra, con co cac furostanol saponin, di tacdung cua enzym (-glucosidase) se chuyen thanhnhom spirostanol saponin.- Saponin triterpen norcycloartanon (I), cycloartanol,cycloartenon va cycloandenol.- T than re ti a phan lap c curcumin.- Nam 1988, phan lap c bis(2 etyl hexyl) phtalatco tac dung sinh hoc (c che s phat trien cua lua


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IV- TAC DUNG VA CONG DUNG- Tac dung chong viem, giam au tren thc nghiem.Hon hp saponin t Ma do co tac dung chong viemro ret, tng ng -methason.- Tac dung gay thu teo tuyen c tren chuot thnghiem.Than re Ma do dung cha sot, tieu buot, tieu vang,viem bang quang, viem than, x gan co chng. Lieu5-10 g/ngay dang thuoc sac.Than re gia ap cha ran can.Ma do co the dung chiet xuat diosgenin. Trc khithuy phan acid, u dc lieu vi enzym -glucosidasee chuyen furostanol saponin thanh spirostanol elam giau diosgenin.


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Cac loai quan trong nhat:Agave sisalana Perr.va Agave fourcroydes Lem. VN co loai A. americana moc hoangHo Thua (Agavaceae)

DA MYDa ba, Cay ThuaSisalLa cua nhieu loai Agave


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I- AC IEM THC VAT- Cay mang hang chuc la moct goc.- Mep la co gai cng, bong vacong, ngon la tan cung bang1 gai nhon.- Khi ra hoa truc hoa mocthang t gia vong la mangnhieu hoa ep.Cay phan bo nhieu ni trenthe gii Mexico, ong Phi, Ano Cay nhap lam canh, nay mochoang dai nhieu ni. Mocc tren at kho can.

Da MAgave sisalana Perr.Ho Thua (Agavaceae)
http://en.wikipedia.org/wiki/Image:Plantsisal.jpg


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I- AC IEM THC VAT

Da MAgave sisalana Perr.Ho Thua (Agavaceae)

Cum hoa
http://www.hear.org/starr/hiplants/images/hires/starr_080210_2760_agave_sisalana.jpghttp://www.hear.org/starr/hiplants/images/hires/starr_070124_3984_agave_sisalana.jpghttp://upload.wikimedia.org/wikipedia/commons/3/3f/Fibresisal.jpg


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II- BO PHAN DUNGLa dung e khai thac lay si va chiet hecogeninIII- THANH PHAN HOA HOCLa cha saponin steroid, trong o hecogenin chiemham lng cao. T loai Thua Viet nam, BM Dclieu Trng H Dc Ha noi a chiet checogenin vi ham lng 0,03 tnh tren la ti.

22 O

CH3O

HOH

O

Hecogenin


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IV- CONG DUNGLa c dung san xuat si lam day thng, detbao b.Chiet hecogenin e tong hp thuoc steroid:thng dung dch la d pham sau khi che biensi. Len men dch la, thu lay chat lang ong nhbun giau sapogenin (5-10 ). Thuy phan tiepbang acid vo c (HCl, H2SO4). Chiet aglyconbang dung moi hu c (heptan, hexan, CH2Cl2 .. .) nong. Tinh che va ket tinh e thu checogenin

Documents

GS Duc - Saponin DL Chua Saponin