GSM; A SUSTAINABLE BIOACTIVES RESOURCE

MATT MILLER

AAOCS 2017 SEPT 2017

GREENSHELL MUSSELS AND CAWTHRON

Seafood safety/Marine toxins

Selective breeding and culturing

Bioactives hub

Energetics and physiology

RE-VALUING THE MUSSEL WITH HVN

• The aim to increase the value of Greenshell

Mussel™ (GSM) based food export products.

This will be achieved by determining the

effect of these GSM products (and bioactives

within) on improving joint and bone health

while reducing inflammation

Cawthron Institute and Sanford Limited

business led research

We aim to develop the evidence dossier to

make a general health claim for GSM food

products

Efficacy trials (in vivo, in vitro and clinical)

GREENSHELL MUSSEL INDUSTRY EXPORTS

2016

Value

(NZD$ -

FOB)

2016

Volume (Kg)

Price

($/KG)

Greenshell

Mussels$311.0m 33,305,1935 ~$7-9

Freeze Dried

Mussel Powder$12.1m 214,937 ~$50

Mussel Oil $43.5m 15,321 $2843

For perspective – Fish oil $2-3/kg, Tuna oil $20-25/kg,

Krill 30-50/kg

WHAT IS IN A GSM?

Lean marine protein

~15 g/100g

Peptides (bioactivity?)

Bioactive lipids

1-2 g/100g

40-45% omega3

EPA 20-25%

DHA10-18%

Other functional bioactive lipids

Functional carbohydrates

~6 g/100g

Glycosaminoglycans

Chondroitin sulphate

Hyaluronic acid

Minerals (vitamins)

selenium, iron,

Vitamin B12 and

iodine

Sustainable source – Algae bioaccumulators

OTHER GSM BIOACTIVE LIPIDS

1) Phytosterols

eg. Fucosterol 2) Non methylene interrupted (NMI) fatty acids

3) Plasmolagens (fatty aldehydes)

4) Fucoxanthin

5) Interesting polar lipids (eg CAEP)

IS A MUSSEL A MUSSEL?

Why

Understand lipid composition of the Mussel

What role gender or season plays

Understand what and when bioactives are present

What

Samples market ready mussels monthly

From Marlborough Sounds

Analyzed them by gender

Developed novel NIR techniques

FAT CONTENT OF GSM OVER 12 MONTHS (G/100G)

OMEGA 3 FATTY ACIDS

Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar

2016 2017

mg

/10

0g

Female

Apr May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar

2016 2017

Male

LIPID CLASS

*

TA

G g

/10

0P

ola

r lip

id g

/10

0

TA

G g

/10

0 o

f lip

idP

ola

r lip

id g

/10

0 o

f lip

id

Female Male

INTERESTING BIOACTIVE LIPIDS

NM

I-F

A g

/10

0 o

f F

A

Pla

sm

alo

ge

n(F

AL

D)g

/10

0 o

f F

A

NIR – NEAR INFRARED SPECTROSCOPY

PROXIMATE ANALYSIS (I.E. PROTEIN, FAT, GLYCOGEN,

MOISTURE PLUS OILS)

Standard (chemical) lab techniques: Food lab

Well established methods

Precise and reproducible

However

Costly (~$200 per sample)

Slow turnaround (eg. week)

Rapid NIR analytical techniques:

Fast (10 seconds), cheap, high throughput

Method development ($$)

Doesn’t need precision of lab methods

Can determine – Fat, protein, carbohydrate,

moisture, ash? EPA? DHA?

BACKGROUND TO NIRS

NIR absorption: associated with molecular vibration

NIR (700 to 2500 nm)

NIR absorption of cookie dough

100 (reference)samples

MODEL DEVELOPMENT (CALIBRATION)

Sample

code%

Fat

%

Protein

%

Glycogen

%

Moisture

A 1.4 13.8 0.8 80.3

B 1.9 7.9 3.0 83.7

C 2.5 12.1 4.0 76.5

D 4.7 12.5 1.2 80.6

…..etc …..etc ….etc ….etc ….etc

Spectral data + Chemical data

Optimising the model :

- define scan region

- mathematical processing

- define # of factors

- outlier removal

Chemometric modelling Develop model

C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-001_F_20160419.0 HVN-001;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-002_F_20160419.0 HVN-002;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-003_F_20160419.0 HVN-003;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-004_M_20160419.0 HVN-004;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-005_M_20160419.0 HVN-005;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-006_M_20160419.0 HVN-006;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-007_F_20160419.0 HVN-007;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-008_F_20160419.0 HVN-008;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-009_F_20160419.0 HVN-009;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-010_M_20160419.0 HVN-010;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-011_M_20160419.0 HVN-011;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-012_M_20160419.0 HVN-012;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-013_MIX_20160419.0 HVN-013;MIX;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-014_MIX_20160419.0 HVN-014;MIX;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN 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HVN-050;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-051_F_20160518.0 HVN-051;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-052_F_20160518.0 HVN-052;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-053_F_20160518.0 HVN-053;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-054_M_20160518.0 HVN-054;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-055_M_20160518.0 HVN-055;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-056_M_20160518.0 HVN-056;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-057_F_20160616.0 HVN 057;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-058_F_20160616.0 HVN 058;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-059_F_20160616.0 HVN 059;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-060_M_20160616.0 HVN 060;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-061_M_20160616.0 HVN 061;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-062_M_20160616.0 HVN 062;M;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-063_F_20160616.0 HVN 063;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration validation\Raw Data Files\HVN Mussels_HVN-064_F_20160616.0 HVN 064;F;;HVN Mussels;Solids C:\Users\Mattm\Desktop\Temp folder\Calibration 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MOISTURE

NIR MODELS OF CHINOOK SALMON

MUSSELING UP PROJECT

In vitro and In vivo trials

Clinical trial (starting 2018)

GSM – worlds most expensive marine oil

Cawthron bioactives hub - delivering tailored services for NZ

biotech companies.