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“Germ Presentation why they are SO BAD!!!!” PHEOCS Investigation By, Zach W, Zach O, Jason H, Samuel W.

H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

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Page 1: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

“Germ Presentation why

they are SO BAD!!!!”

PHEOCS Investigation

By, Zach W, Zach O, Jason

H, Samuel W.

Page 2: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Background Information

We are doing this to investigate germs. the different kinds, where they can be found,

where more germs are then other germs. Here are some facts that we have learned

through our research.

1. what are germs?

Its a very small microorganism that can spread throughout the world. they are hard to see

which makes it more dangerous.

2. What do Germs do?

Germs can Cause Diseases and sickness after they get in you and infect your body with

germs.

3. Where do Germs Live?

Germs live in or on dirt, water, and countertops. Germs live anywhere where an

livingbeen lives and then they can spread then keep spreading then next thing you know

they are all over the world infecting the world.

4. How do Germs Spread?

Germs need to get into your body to spread throughout your body they also can travel

through the air from the wind and by sporing more of itself.

5. How can you Prevent the Spread of Germs?

You can prevent the spread of Germs by Washing your hands after you go to the

bathroom or before you eat everyday or clean your body.

Page 3: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Expert Questions

Questions for our Expert Dr. Lawrence Donatelle

1.Where do you think germs are found most in school? 2.What are some tips you could give us about staying germ free? 3.Can germs spread on their own? 4.How long do most germs live? 5.What is a germ? 6.In your opinion which has more germs, a bathroom or a classroom?

Page 4: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Expert Answers

Questions for our Expert Dr. Lawrence Donatelle

1. Desktops or Mice. 2. Wash hands often, use hand sanitizer, clean areas with alcohol wipes. 3. No but if you touch an infected area the germs are on your hands and will spread to where ever you touch. 4. Most live for one to two days 5.A germ can be a bacteria, virus or mold/fungi;. Because viruses arent living they can be living things or not. 6. Classroom

Page 5: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Hypothesis

The bacteria from the different rooms is going to

grow up to about the size that a human eye can

see.

A class room chair will have the most bacteria

on it because it is the most used room in the

school.

the chair has a lot of Germs in that area of the

chair will be very dirty

Tables/desk will have the least amount of germs

Page 6: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Designing experiment

1. 6 Petri Dishes (4 inch size)

2. Agar Nutrient (5 Grams)

3. Hand Sanitizer

4. 6 Cotton Swabs

5. Beaker to Boil Water

6. Water

7.Zipper-Lock Bag

Page 7: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Designing experiment

Step 1 Wash each petri dish in hot, soapy water before you use it.

Step 2 Do not place the petri dish in boiling water or dishwasher with

heated dry turned on.

Step 3 Make sure the dish is sterile before you get started. And to

make sure nobody touches it.

Step 4 Next grab the bag of nutrient agar (awger).

Step 5 You will need a clean microwave safe container to mix and heat

the agar with water.

Step 6 Mix the entire bag of agar (about 5 grams) with one cup

(240mL) of hot water and stir. then put it in microwave and bring it to a

boil.

Step 7 Bring this mixture to a boil for 1 min to completely dissolve the

agar.

Step 8 If you are using the microwave oven to boil the mixture be

careful not to let the solution boil over.

Page 8: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Designing experiment

Step 9 The mixture should be clear, no particles should be floating around

the solution.

Step 10 Allow the mixture to cool for 3 to 5 minutes before moving onto the

next step.

Step 11 Fill the bottom half of the petri dish one half full with the warm

nutrient solution.

Step 12 Cover each petri dish with the lid and allow the solution to cool and

harden for at least an hour.

Step 13 Choose the items that you will be taking a bacteria sample of with a

cotton swab.

Step 14 Clean the cotton swab by rinsing it with water.

Step 15 Collect some bacteria on the end of a cotton swab from your

selected item or location.

Step 16 Remove lid from petri dish and draw a squiggly line on it with agar.

Step 17 Label the petri dish with what was swabbed.

Step 18 Keep track of the results.

Step 19 Repeat steps 13 through 18 for different items or locations

Page 9: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Data Table

Location Bathroom

Stall Toilet

Seat

8th Grade

Keyboard

CellPhone

7th Grade

Chair

Door Handle

Table

Images from

your room that

you chose

Date and Time

of project

monday jan

28 10:15

monday jan

28 10:16

monday jan

28 10:17

monday

jan 28

10:18

monday jan

28 10:19

monday

jan 28

10:20

Description of

the chosen room

A toilet seat

in the 8th

grade wing

A keyboard

in the 8th

grade

computer lab

Sam Ws

phone

A chair in

the 7

grade

wing

A door

handle by

the

guidance

room.

A desk by

the

guidance

room

How much

Bacteria

Day 6

Page 10: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Data Table

Location Bathroom

Stall Toilet

Seat

8th Grade

Keyboard

CellPhone

7th Grade

Chair

Door Handle

Table

Images from

your room that

you chose

Date and Time

of project

monday jan

28 10:15

monday jan

28 10:16

monday jan

28 10:17

monday

jan 28

10:18

monday jan

28 10:19

monday

jan 28

10:20

Description of

the chosen room

A toilet seat

in the 8th

grade wing

A keyboard

in the 8th

grade

computer lab

Sam Ws

phone

A chair in

the 7

grade

wing

A door

handle by

the

guidance

room.

A desk by

the

guidance

room

How much

Bacteria

Day 8

Page 11: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Conclusions

Bacteria can grow that your eye can see. A chair is have the more bacteria than a toilet. The dirtiest part of a chair is the back.

Page 12: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Observations

We could of worked together better. And got more work done

Page 13: H, Samuel W PHEOCS Investigation W...PHEOCS Designing experiment Step 1 Wash each petri dish in hot, soapy water before you use it. Step 2 Do not place the petri dish in boiling water

PHEOCS Cite resources

http://beediverse.com/products-page/accessories/933-petri-dishes-three/

http://www.ebay.com/itm/DEHYDRATED-NUTRIENT-AGAR-10-g-

/330327365964

msugradwellness.wordpress.com

http://.forums.gametrailers.com

http://www.wisegeek.com/what-is-purified-water.htm#slideshow

http://www.webstaurantstore.com/plastic-bag-8-x-8-zip-lock-100-pack-

heavy-weight-freezer/130F40808%20%20%20100.html

Websites