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Haematology and Blood Transfusion 28----------------------------------Hamatologie und Bluttransfusion
Edited by H. Heimpel, VIm· D. Huhn, Munchen C. Mueller-Eckhardt, GieSen G. Ruhenstroth-Bauer, Munchen
Modern Trends in Human Leukemia V New Results in Clinical and Biological Research Including Pediatric Oncology
Organized on behalf of the Deutsche Gesellschaft flir Hamatologie und Onkologie. Wilsede, June 21-23, 1982
Wilsede Joint Meeting on Pediatric Oncology II Hamburg, June 25126, 1982
Edited by R. N eth, R. C. Gallo, M. F. Greaves, M. A. S. Moore, and K. Winkler
With 192 Figures and 151 Tables
Springer-Verlag Berlin Heidelberg New York Tokyo 1983
Dr. RolfNeth, Universitaets-Krankenhaus Eppendorf, Medizinische Klinik, Abteilung fuer klinische Chemie, MartinistraBe 52, 2000 Hamburg 20, Federal Republic of Germany Dr. Robert C. Gallo, National Cancer Institute, Laboratory of Tumor Cell Biology, Bethesda, MD 20014, USA Dr. Melvyn F. Greaves, Imperial Cancer Research Fund Laboratories, P.O. Box 123, London WC2 A3PK, Great Britain Dr. Malcolm A. S. Moore, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA Dr. Kurt Winkler, Universitaets-Kinderklinik, Abteilung fuer Haematologie und Onkologie, MartinistraBe 52, 2000 Hamburg 20, Federal Republic of Germany
Supplement to BLUT - Journal Experimental and Clinical Hematology Organ of the Deutsche Gesellschaft fUr Hiimatologie und Onkologie der Deutschen Gesellschaft fUr Bluttransfusion und Immunhiimatologie and of the Osterreichische GesellschaftfUr Hiimatologie und Onkologie
ISBN -13: 978-3-540-11858-9 e-ISBN -13: 978-3-642-68761-7 DOl: 10.1007/978-3-642-68761-7
Library of Congress Cataloging in Publication Data. Main entry under title: Modern trends in human leukemia V. (Haematology and blood transfusion = Hlimatologie und Bluttransfusion; 78) "Organized on behalf of the Deutsche Gesellschaft flir Hlimatologie und Onkologie and the Deutsches Krebsforschungszentrum, Wilsede, June 1982." "Supplement to Blut." Bibliography: p. Includes index. I. Leukemia-Congresses. 2. Leukemia in children-Congresses. I. Neth, Rolf. II. Deutsche Gesellschaft flir Hlimatologie und Onkologie. III. Deutsches Krebsforschungszentrum Heidelberg. IV. Series: Haematology and blood transfusion; 78. [DNLM: I. Leukemia-Congresses. WI HAI655 v. 281 WH 250 M689 1982) RC643.M62 1983 616.99'419 83-505 ISBN -13: 978-3-540-11858-9 (U.S.)
This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned specifically those of translation, reprinting, re-use ofillustrations, broadcasting, reproduction by photocopying machine or similar means, and storage in data banks. Under § 54 of the German Copyright Law where copies are made for other than private use a fee is payable to "Verwertungsgesellschaft Wort", Munich.
© Springer-Verlag Berlin Heidelberg 1983
The use of registered names, trademarks etc. in this publication does not imply even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use.
Product Liability: The publisher can give no guarantee for information about drug dosage and application thereof contained in this book. In every individual case the respective user must check its accuracy by consulting" other pharmaceutical literature.
2123/3321/543210
Scientific Organisation of the Sessions
New Strategies in Leukemia Diagnostic and Therapy
Heimpel, Hermann, Zentrum fuer Innere Medizin und Kinderheilkunde der Universitaet Ulm, Steinhoevelstrasse 9, 7900 Ulm, Federal Republic of Germany Hoflbrand, A. Viktor, Dept. of Haematology, Royal Free Hospital, Rond Street, Hampstead, London NW32QG, Great Britain
Mechanism of Malignant Transformation Virology
Gallo, Robert, National Cancer Institute, Bldg. 37, Room 6B04, Bethesda, MA 20014, USA Hausen, Harald zur, Institut fuer Virologie im Zentrum fuer Hygiene, Hermann-Herder-Strasse 11, 7800 Freiburg, Federal Republic of Germany
Cellbiology
Greaves, Melvyn F., Imperial Cancer Research Fund Laboratories, P.O. Box 123, London WC2A3PX, Great Britain Moore, Malcolm A.S., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Immnnological Aspects in Malignancy
Cooper, Max, University of Alabama, School of Medicine, Comprehensive Cancer Center, Birmingham, AL 35294, USA Mitchison, N. Avrion, University College London, Tumour Immunology Unit, Gower Street, London WCIE6BT, Great Britain
Wilsede Joint Meeting on Pediatric Oncology II
Kabisch, Hartmut, Universitaetskinderklinik, Abteilung flir Haematologie und Onkologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany Winkler, Kurt, Universitaetskinderklinik, Abteilung flir Haematologie und Onkologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
v
Local Organisation
Mannweiler, Klaus, Heinrich-Pette-Institut fuer experimentelle Virologie und Immunologie an der Universitaet Hamburg, Martinistrasse 52,2000 Hamburg 20, Federal Republic of Germany Neth, Rolf, Universitaetskrankenhaus Eppendorf, Medizinische Klinik, Abteilung fuer klinische Chemie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
VI
Contents
Participants of the Meeting 0
Wilsede Scholarship Holders Preface 0 0 0 0 0
Acknowledgment 0 0 0 0 0
Frederick Stohlman Jr. Memorial Lecture
XVII XXIII XXV
XXIX
Greaves, Mo Fo: Immunobiology of Lymphoid Malignancy 0 3
Thomas, Eo Do: Bone Marrow Transplantation in Leukemia 1 0 11
New Strategies in Diagnosis and Therapy
Hofibrand, Ao Vo, Ma, Do Do F., Prentice, Ho Go: Nucleotide Metabolism and Enzyme Inhibitors in Thymic Acute Lym-phoblastic Leukaemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 19
McCaffrey, Ro, Bell, Ro, Lillquist, A., Wright, Go, Baril, Eo, Minowada, Jo: Selective Killing of Leukemia Cells by Inhibition ofTdT . 0 • 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 24
Papamichail, Mo, Trangas, T., Courtis, No, Ioannides, c., Cosmidou, Ho, Pangalis, Go A., Tsiapalis, C. Mo: Poly(A)-poly-merase Levels in Leukemia 0 0 0 • 0 0 0 0 0 0 • 0 0 0 0 28
Lister, T. A., Rohatiner, A. C. So, Slevin, Mo L., Dhaliwal, Ho So, Bell, Ro, Henry, Go, Thomas, Ho, Amess, Jo: Short-term Therapy for Acute Myelogenous Leukaemia in Younger Pa-tients 2
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 30
Hoelzer, Do, Thiel, Eo, Loffier, Ho, Bodenstein, Ho, BUchner, To, Messerer, Do, for the German Multicentre ALL! AUL Study Group: Multicentre Pilot Study for Therapy of Acute Lymphoblastic and Acute Undifferentiated Leukaemia in Adults 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 36
Weinstein, Ho Jo, Mayer, Ro Jo, Rosenthal, Do So, Coral, Fo So, Camitta, B. Mo, Gelber, R. Do, Nathan, Do Go, Frei, Eo III: The Treatment of Acute Myelogenous Leukemia in Children andAduIts:VAPAUpdate 2
00000000000000 41
VII
Creutzig, u., Schellong, G., Ritter, J., Sutor, A H., Riehm, H., Langermann, H. J., Jobke, A, Kabisch, H.: Improved Results in Treatment of Acute Myelogenous Leukemia in Children -Report of the German Cooperative AML Study BFM-78 2 ., 46
Chesseis, J. M., Sieff, C. A, Rankin, A: Acute Myeloid Leu-kaemia in Childhood: Treatment in the United Kingdom 2 •• 51
Rohatiner, A Z. S., Balkwill, F., Lister, T. A: Interferon in Acute Myelogenous Leukaemia: A Preliminary Report . .. 56
Siegert, W., Huhn, D., Theml, H., Fink, U., Kaudewitz, P., Riethmuller, G., Wilmanns, W.: Human Interferon-Beta in the Treatment of Non-Hodgkin Lymphoma ....... 59
Zintl, F., Herman, J., Katenkamp, D., Malke, H., Plenert, W.: Results of LSA2-L2 Therapy in Children with High-Risk Acute Lymphoblastic Leukemia and Non-Hodgkin's Lym-phoma . . . . . . . . . . . . . . . . . . . . . . .. 62
Ramot, B., Ben-Bassat, I., Many, A, Kende, G., Neuman, Y., Brok-Simoni, F., Rosenthal, E.: Clinical and Epidemiological Observations on Acute Lymphoblastic Leukemia Subtypes at the Sheba Medical Center, Israel .......... 67
Slevin, M. L., Piall, E. M., Aherne, G. W., Johnston, A., Lister, T. A: The Clinical Pharmacology of Cytosine Arabi-noside . . . . . . . . . . . . . . . . . . . . . . .. 70
Erhardt, J., Emmerich, B., Theml, H., Riethmuller, G., Ziegler-Heitbrock, H. W. L.: Enhancement of the Cell-Mediated Lysis of Fresh Human Leukemia Cells by Cytostatic Drugs.. 76
Wilson, E. L., Jacobs, P., Dowdle, E. B.: Secretion of Plasminogen Activators by Human Myeloid Leukemic Cells: Modulation and Therapeutic Correlations ........ 78
Wilms, K., Schuch, K., Muller, C., Link, H., Meyer, P., Ostendorf, P., Waller, H. D., Wernet, P., Niethammer, D., Dopfer, H., Schneider, W., Schunter, F., Breitling, G., Frommhold, W., Seeger, G., Voss, A C., Rodt, H.: Bone Marrow Trans-plantation in West Germany in Patients with Leukemia 3. •• 81
Powles, R. L., Morgenstern, G. R., Leigh, M., Filshie, J., Platt, J., Lumley, H.: Cyc1osporinA Following Matched and Mis-matched Family Allogeneic Bone Marrow Transplants 2 • •• 87
Kaizer, H., Tutschka, P., Stuart, R., Korbling, M., Braine, H., Saral, R., Colvin, M., Santos, G.: Autologous Bone Marrow Transplantation in Acute Leukemia and Non-Hodgkin's Lymphoma: A Phase I Study of 4-Hydroperoxycyc1ophosphamide (4HC) Incubation of Marrow prior to Cryopreser-vation 2. • . • • . • . . . . . . • • . . . . . • • •. 90
VIII
Rodt, Ho, Thierfelder, So, Bender-Gotze, Co, Dopfer, Ro, Haas, Fo Jo, Janka, Go, Kolb, Ho Jo, Link, Ho, Netzel, Bo, Niethammer, Do, Schiich, K., Wilms, K.: Serological Inhibition of Graft Versus Host Disease: Recent Results in 28 Patients with Leukemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Tutschka, Po Jo: Graft-Versus-Host Disease: Immunobiologi-cal Aspects 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Stiinkel, K., Bender-Gotze, c., Netzel, B., Thiel, Eo: T-Cell Phenotypes in Mixed Leukocyte Reactions and After Bone Marrow Transplantation: Are la-Antigen on T-Cells a Marker for GvH Reactions? 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Thorpe, Po Eo, Detre, So I., Mason, Do Woo Cumber, A Jo, Ross, Wo c.: Monoclonal Antibody Therapy: "Model" Experiments with Toxin-Conjugated Antibodies in Mice and Rats
LeBien, To Wo, Ash, R c., Zanjani, Eo Do, Kersey, Jo Ho: In Vitro Cytodestruction of Leukemic Cells in Human Bone Marrow Using a Cocktail of Monoclonal Antibodies
Ritz, Jo, Sallan, So Eo, Bast, R Co Jr., Lipton, Jo Mo, Nathan, Do Go, Schlossman, So Fo: In Vitro Treatment with Monoclonal Antibody Prior to Autologous Bone Marrow Transplantation in Acute Lymphoblastic Leukemia 2 0 0 0 0 0 0 0 0
Chen, Po-Mo, Ho, C.-Ko: Immunological Classification of Acute Lymphatic Leukemia 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Thoene, I., Kabisch, Ho: Multimarker Analysis of Childhood Acute· Lymphoblastic Leukemia (ALL): Heterogeneity of Cellular Phenotypes and Clinical Relevance of Immunologi-cal Defined ALL Subclasses 0 0 0 0 0 0 0
Crockard, A Do, O'Brien, Mo, Robinson, Do, Tavares de Castro, Jo, Matutes, Eo, Catovsky, Do: Morphological and C~tochemical Features of Adult T-Cell Lymphoma-Leukae-mIa 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Augener, Wo, Brittinger, Go, Schiphorst, Wo Eo C. Mo, van den Eijnden, Do Ho: Activities and Specificities of N-Acetylneuraminyltransferases in Leukemic Cell 0 0 0 0 0 0 0 0 0 0 0
Tax, Wo Jo Mo, Greaves, Mo Fo, Willems, Ho Mo, Leeuwenberg, Ho Fo Mo, Capel, Po Jo A, Koene, R A Po: WTl: A Monoclonal Antibody Reactive with T -ALL but not with Other Leukemias 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bell, Ro, Lillquist, A, Cotter, So, Sallan, So, McCaffrey, R: Dysfunctional Glucocorticoid Receptors in Acute Leukemia 0
Galili, No, Galili, Uo: Glucocorticoid-Induced Lysis of Various Subsets of Acute Lymphoblastic Leukemia 0 0 0 0 0 0 0 0
92
97
102
107
112
117
124
126
131
135
139
142
146
IX
Blattner, W. A, Blayney, D. W., Jaffe, E. S., Robert-Guroff, M., Kalyanaraman, V. S., Gallo, R. c.: Epidemiology of HTL V -Associated Leukemia . . . ~. . . . . . . . . . . 148
Henderson, E. S.: Current Prospects for Clinical Care of Acute Leukemia. . . . . . . . . . . . . . . . . . . . 156
Mechanism of Malignant Transformation
Duesberg, P., Nunn, M., Biehl, T., Phares, W., Lee, W.-H.: Viral Oncogenes and Cellular Prototypes . . . . . . . . . 163
Bister, K., Enrietto, P., Graf, T., Hayman, M.: The Transform-ing Gene of Avian Acute Leukemia Virus MC29 ..... l73
Wong-Staal, F., Josephs, S., Dalla Favera, R., Westin, E., Gelmann, E., Franchini, G., Gallo, R. c.: Cellular one Genes: Their Role as Progenitors of Viral one Genes and Their Expression in Human Cells . . . . . . . . . . . . . . . . 178
Anders, F.: The Biology of an Oncogene, Based Upon Studies on Neoplasia in Xiphophorus . . . . . . . . . . . . . . 186
Papas, T. S., Rushlow, K. E., Watson, D. K., Bader, 1. P., Ray, D., Reddy, E. P.: The Transforming Gene of Avian Myeloblastosis Virus (AMV): Nucleotide Sequence Analysis and Identification ofIts Translational Product ........ 207
Moelling, K., Greiser-Wilke, 1., Owada, M. K., Donner, P., Bunte, T.: DNA-Binding Ability of Transforming Proteins from Avian Erythroblastosis Virus and Mutant Avian Myelocytomatosis Virus, MC29, in Comparison with MC29 Wild Type ........................ 214
Kettmann, R., Westin, E. H., Marbaix, G., Deschamps, J., Wong-Staal, F., Gallo, R. c., Bumy, A: Lack of Expression of Cellular Homologues of Retroviral one Genes in Bovine Tumors .. . . . . . . . . . . . . . . . . . . . . . 218
Bruck, c., Portetelle, D., Zavada, J., Bumy, A: Molecular Dissection of the Bovine Leukemia Virus Envelope Glycopro-tein (BL V gp5l) by a Monoclonal Antibody Study 222
Chen, A P., Essex, M., de Noronha, F.: Detection and Localization of a Phosphotyrosine-Containing one Gene Product in Feline Tumor Cells . . . . . . . . . . . . . . . . . . 227
Scott, M. R. D., Brickell, P. M., Latchman, D. S., Murphy, D., Westphal, K.-H., Rigby, P. W. J.: The Use of cDNA Cloning Techniques to Isolate Genes Activated in Tumour Cells 236
Lane, M. A, Sainten, A., Neary, D., Becker, D., Cooper, G. M.: Cellular Transforming Genes in Cancer ....... 241
x
Dalla Favera, R., Westin, E., Gelmann, E. P., Martinotti, S., Bregni, M., Wong-Staal, F., Gallo, R. c.: The Human one Gene e-mye: Structure, Expression, and Amplification in the Human Promyelocytic Leukemia Cell Line HL-60 247
Haseltine, W. A., Lenz, J., Crowther, R.: Determination of Virulence Properties of Leukemia Viruses ........ 255
Fischinger, P. J., Dunlop, N. M., Poore, C. M., Robey, W. G.: Generation of Recombinant Murine Leukemia Viruses De Novo: An Alternative Model for Leukemogenesis ..... 261
Jaenisch, R., Harbers, K., Jahner, D., Stewart, C., Stuhlmann, H.: Expression of Retroviruses During Early Mouse Embryogenesis . . . . . . . . . . . . . . . . . . . . . . . . 270
Chieco-Bianchi, L., D'Andrea, E. D., De Rossi, A., Zanovello, P., Ronchese, F., Collavo, D.: Natural History of M-MSV Tumors in Mice Carrying Endogenized Moloney Leukemia Virus. . . . . . . . . . . . . . . . . . . . . . . . . 275
Nobis, P., L6hler, J.: Induction of Histiocytomas by Pristane Treatment of Mice Chronically Infected with Moloney Mu-rine Leukemia Virus . . . . . . . . . . . . . . . . . . 280
Jaquemin, P. c.: Purification of a Reverse Transcriptase-like Protein from the Plasma of a Patient with Chronic Myeloge-nous Leukemia and Production of Monoclonal Antibodies 282
Schetters, H., ErfIe, V., Hehlmann, R.: Detection of Group and Interspecies Reactivities in Mammalian C-Type Virus p30 Proteins and Corresponding Human Antigens 284
Hausen, H. zur: Papovaviruses and Human Tumors 289
Modrow, S., Schmidt, H., Wolf, H.: Characterization of Pro-teins Induced by Herpesvirus saimiri: Comparative Immunoprecipitation and Analysis of Glycosylation . . . . . . . . 293
Bornkamm, G. W., Freese, U. K., Laux, G., Hudewentz, J., Delius, H.: Structural Organization and Expression of the Epstein-Barr Virus Genome . . . . . . . . . . . . . . . . 295
Bauer, G., Hausen, H. zur: Induction of Latent Epstein-Barr Virus Information by a Serum Factor .......... 297
Boniver, J., Houben-Defresne, M. P.: Thymic Nurse Cells and Radiation Leukemia Virus Induced Thymic Lymphomas in C57BL Mice . . . . . . . . . . . . . . . . . . . . . 299
Young, B. D., Jeanpierre, M., Goyns, M. H., Stewart, G. D., Elliot, T., Krumlauf, R.: Construction and Characterization of Chromosomal DNA Libraries . . . . . . . . . . . . . . 301
XI
Gallo, R. c., Popovic, M., Sarin, P., Reitz, M. S. Jr., Kalyanaraman, V. S., Aoki, T., Sarngadharan, M. G., Wong-Staal, F.: Human T-Cell Leukemia-Lymphoma Virus (HTLV): A Pro-gress Report . . . . . . . . . . . . . . . . . . . . . 311
Metcalf, D.: Early Events in the Suppression of Myeloid Leu-kemic Cells by Biological Regulators .......... 320
Moore, M. AS., Gabrilove, J., Sheridan, A P.: Myeloid Leukemic Cell Differentiation Induced by Human Postendotoxin Serum and Vitamin Analogues ............. 327
Burgess, A W., Cooper, P. c., Stanley, I. J., Nicola, N. A: Ef-fect of Colony-stimulating Factors on the Proteins Synthe-sized by Normal and Leukemic Myeloid Progenitor Cells 338
Nicola, N. A, Matsumoto, M., Metcalf, D., Johnson, G. R.: Molecular Properties of a Factor Inducing Differentiation in Murine Myelomonocytic Leukemic Cells . . . . . . . . . 345
Minowada, J., Minato, K., Tatsumi, E., Sugimoto, T., Nakaza-wa, S., Ohnuma, T., Kubonishi, I., Miyoshi, I., Frankel, A, Gallo, R. c.: A Model Scheme for Human Hematopoietic Cell Differentiation as Determined by Multiple Markers of Leukemia-Lymphomas ................ 348
Singer, J. W., Keating, A.: Studies on the In Vitro Microenvironment in Man . . . . . . . . . . . . . . . . . . . . 351
Francis, G. E., Guimaraes, J. E., Berney, J. J., Granger, S., Hoflbrand, A V.: Granulocyte-Macrophage Progenitor Cell Proliferation and Differentiation . . . . . . . . . . . . . 355
Elstner, E., Schulze, E., Ihle, R., Schlitt, M., Stobbe, H.: Proliferation and Maturation of Hemopoietic Cells from Patients with Preleukemia and Aplastic Anemia in Agar and Diffusion Chamber Cultures . . . . . . . . . . . . . . . . . . . 358
Rabe, K., Rehpenning, W., Winkler, K., Heinisch, B., Krause, U., Soltau, H., Neth, R.: Persistent Deficiency of Myeloperoxidase and Lactoferrin in Granulopoietic Cells of Patients with Acute Leukemia ................. 362
Izaguirre, C. A, Greaves, M. F.: Studies on Normal B-Cells and Common Acute Lymphoblastic Leu~emia Blast Cells Using a Colony Assay . . . . . . . . . . . . . . . . . 366
Welte, K., Venuta, S., Wang, C. Y., Feldman, S. P., Ciobanu, N., Kruger, G., Feickert, H. J., Merluzzi, V. J., Flomenberg, N., Moore, M. AS., Mertelsmann, R.: Human Interleukin 2: Physiology, Biochemistry, and Pathophysiology in Lympho-blastic Leukemias and Immunodeficiency Syndromes 369
Brown, G., Fisher, A G., Bunce, C. M., Stone, P. C. W., Tok-soz, D.: Studies of Myelopoiesis Using Monoclonal Antibodies and Variant Lines from the Pro myeloid Cell Line HL60 . . . . . . . . . . . . . . . . . . . . . . . . 380
XII
Olsson, I. L., Breitman, T. R., Sarngadharan, M. G., Gallo, R. c.: Mechanisms for Induction of Differentiation in the Hu-man Promyelocytic Cell Line HL-60' . . . . . . . . . . 384
Uchailska-Ziegler, B., Wernet, P., Ziegler, A: Differentiation of a Human Myeloid Cell Line (HL-60) Toward Granulocyteand Macrophage-like Cells: Comparison of Cell Surface Anti-gen Expression . . . . . . . . . . . . . . . . . . . . 386
Sarin, P. S., Popovic, M., Salahuddin, S. Z., Richardson, E., Lange Wantzin, G., Karmarsky, B., Gallo, R. c.: Transmission of Human T-Cell Leukemia Virus (HTLV) into Human Cord Blood T Cells .................. 389
Lau, B., Jager, G., Korge, E., Dormer, P.: K 562 Cell Line in Plasma Clot Diffusion Chambers: Changes in Cell Surface Phenotype in Relationship to Culture Conditions ..... 394
Sieff, c., Bicknell, D., Caine, G., Edwards, P. A W., Greaves, M.: Antigen Expression on Normal and Leukaemic Erythroid Precursors. . . . . . . . . . . . . . . . . . . . . . . 397
Peschle, c., Rossi, G. B., Covelli, A, Migliaccio, G., Migliaccio, A. R., Mastroberardino, G.: The Early Stage of Friend Virus Erythroleukemias: Mechanisms Underlying BPA-"indepen-dent" In Vitro Growth ofBFU-E ............ 403
Stein, H., Gerdes, J., Schwab, U., Lemke, H., Diehl, V.: Evidence for the Origin of Hodgkin and Sternberg-Reed Cells from a Newly Detected Small Cell Population 407
Diehl, V., Burrichter, H., Schaadt, M., Kirchner, H. H., Fonatsch, c., Stein, H., Gerdes, J., Heit, W., Ziegler, A: Hodgkin's Disease Cell Lines: Characteristics and Biological Activities . . . . . . . . . . . . . . . . . . . . . . . 411
Lennert, K., Schmid, u.: Prelymphoma, Early Lymphoma, and Manifest Lymphoma in Immunosialadenitis (Sjogren's Syndrome) - A Model of Lymphomagenesis ....... 418
Immunological Aspects in Malignancy
Cooper, M. D., Kubagawa, H.: B-Cell Malignancies: Origin and Extent of Clonal Involvement ........... 425
Fortunato, A, James, R. F. L., Mellor, A, Mitchison, N. A: Transfection as an Approach to Understanding Membrane Glycoproteins .................... 434
Terhorst, c., Borst, J., Lerch, P., van de Rijn, M., Snow, P., Spits, H., de Vries, J.: Structural and Functional Aspects of the T-Cell Differentiation Antigens T3, T6, and T8 440
Ploegh, H. L.: Human Histocompatibility Antigens 447
XIII
Weiss, E. H., Mellor, A L., Golden, L., Bud, H., Hurst, J., Flavell, R. A., Simpson, E., James, R., Townsend, A. R. M., Festenstein, H.: Structure and Expression of Class I Genes of the Mouse Major Histocompatibility Complex ...... 454
Reitz, M. S., Mann, D., Clarke, M. F., Kalyanaraman, V. S., Robert-Guroff, M., Popovic, M., Gallo, R. c.: Presence of HTL V in a Subset of T Cells from an Infected Patient: Some Immunochemical Properties of the Infected Cells 459
Ziegler, A, Uchanska-Ziegler, B., Wernet, P., Zeuthen, J.: Hybrids Between Human Cell Lines Belonging to Different Hematopoietic Pathways: Analysis of HLA and Myeloid Sur-face Antigens . . . . . . . . . . . . . . . . . . . . . 462
Gidlund, M., Nilsson, K., Totterman, T., Wigzell, H.: Natural Killer Cells and Their Targets: Impact of Differentiation on Target Cell Susceptibility . . . . . . . . . . . . . . . . 466
Ehrlich, R., Efrati, M., Gonen, B., Shochat, L., Witz, I. P.: Natural Cellular Defense Activities Against Tumors - Cytostasis and NK Activity . . . . . . . . . . . . . . . . 470
Vodinelich, L., Schneider, c., Sutherland, D. R., Newman, R. A, Greaves, M. F.: Structure and Function of the Transferrin Receptor - A Possible Role in the Recognition of Natural Killer Cells . . . . . . . . . . . . . . . . . . 472
Milleck, J., Jantscheff, P., Irro, F., Tkatscheva, N., Le Dinh Hoe: NK and K Cells in Malignant Lymph Nodes 475
Green, D. R., Gershon, R. K.: Contrasuppression, Class I An-tigens, and Cancer Immunity . . . . . . . . . . . 479
Flood, P. M., DeLeo, A B., Old, L. J., Gershon, R. K.: Inhibi-tion of the Induction of Contrasuppression by Antisera Against Tumor-Associated Surface Antigens on Methylcholanthrene-induced Sarcomas . . . . . . . . . . . . . 486
Wortzel, R. D., Philipps, c., Urban, J. L., Schreiber, H.: Dissection of a Unique Tumor-Specific Transplantation Antigen into Multiple Unique Independent Epitopes using Syngeneic T -Cell Lines . . . . . . . . . . . . . . . . 489
Rohmer, H., Schetters, H., Luz, A., Hehlmann, R., Erfie, V.: Detection of Antigen-(AKR MuL V gp70)-Specific Circulating Immune Complexes (CIC) in Mice with Lymphomas 493
Sarngadharan, M. G., Schuepbach, J., Kalyanaraman, V. S., Robert-Guroff, M., Oroszlan, S., Gallo, R. c.: Immunological Characterization of the Natural Antibodies to Human T-Cell Leukemia Virus in Human Sera ............ 498
XIV
General Summary
Levy, 10 Po: General Summary of the Meeting 507
Subject Index 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 515
1 Also special Lecture for the Wilsede Joint Meeting on Pediatric Oncology II
2 Were also presented in the Wilsede Joint Meeting on Pediatric Oncology II 3 Presented in the Wilsede Joint Meeting on Pediatric Oncology II
xv
Participants of the Meeting
Anders, Fritz, Genetisches Institut der Universitaet, Heinrich-ButT-Ring 58-62,6300 Giessen, Federal Republic of Germany
Bauer, Georg, Institut fuer Virologie im Zentrum fUr Hygiene, HermannHerder-Strasse 11,7800 Freiburg, Federal Republic of Germany
Bell, Richard, Medical Oncology University Hospital, 75 East Newton Street, Boston, MA 02062, USA
Bernhard, Silke, Dahlem-Konferenzen, Wallotstrasse 19, 1000 Berlin 33, Federal Republic of Germany
Bister, Klaus, Max-Planck-Institut fUr Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33, Federal Republic of Germany
Blattner, William A., Family Studies Section, Environmental Epidemiology Branch, National Cancer Institute, Landow Building, Rm. 4C18, 7910 Woodmont Avenue, Bethesda, MD 20205, USA
Boiron, Michel, Institut de Recherches sur les Leucemies et les Maladies du Sang, Universite Paris VII, Hopital Saint-Louis, 2 Place du Docteur-Fournier, 75475 Paris Cedex 10, France
Boniver, Jacques, Institut de Pathologie B 23, Laboratoire d'Anatomie, Pathologique, 4000 Liege, Belgium
Bornkamm, Georg W., Institut fuer Virologie im Zentrum fuer Hygiene, Hermann-Herder-Strasse 11,7800 Freiburg, Federal Republic of Germany
Burgess, Antony W., Ludwid Institute for Cancer Research, Melbourne Tumour Biology Unit, Royal Melbourne Hospital, Victoria 3050, Australia
Burny, Arsene, Faculte des Sciences, Laboratoire de Chimie Biologique, 67 Rue des Chevauc, 1640 Rhode-St-Genese, Belgium
Chen, Po-min, Veterans General Hospital, Dept. of Hematology, Taipei, Taiwan 112, Republic of China
Chessels, Judith M., The Hospital for Sick Children, Dept. of Haematology and Oncology, Great Ormond Street, London WCIN3JH, Great Britain
Chieco-Bianchi, Luigi, University of Padova, Dept. of Oncology, Via A. Gabelli 61, 351000 Padova, Italy
Cooper, Max D., UAB, Comprehensive Cancer Center, Cellular Immunobiology Unit, Dept. of Pediatrics and Microbiology, Birmingham, AL 35294, USA
Creutzig, Ursula, Universitaets-Kinderklinik, Robert-Koch-Strasse 31, 4400 Muenster, Federal Republic of Germany
Dalla Favera, Riccardo, National Cancer Institute, Building 37, Rm. 6 C04, Bethesda, MD 20014, USA
Dautry, Fran<,:ois, MIT, Center for Cancer Research, 77 Massachusetts Avenue, Boston, MA 02139, USA
Deinhardt, Friedrich, Max-v.-Pettenkofer-Institut, Pettenkoferstrasse 9a, 8000 Muenchen 2, Federal Republic of Germany
Diehl, Volker, Medizinische Hochschule Hannover, Zentrum Innere Medizin und Dermatologie, Karl-Wiechert-Allee 9, 3000 Hannover 61, Federal Republic of Germany
XVII
Duesberg, Peter, Dept. of Molecular Biology, University of California, Wendell M. Stanley Hall, Berkeley, CA 94720, USA
Ehrlich, Rachel, George S. Wise Faculty of Life Sciences, Dept. of Microbiology, Tel Aviv, Israel
Elstner, Elena, Universitaetsklinik fuer Innere Medizin, Charite, Schumannstrasse 20121, 1040 Berlin, German Democratic Republic
Erfie, Volker, Gesellschaft fuer Strahlen- und Umweltschaeden, Institut flir Biologie, 8042 Neuherberg, Federal Republic of Germany
Essex, Max, Harvard School of Public Health, Dept. of Microbiology, 665 Huntington Avenue, Boston, MA 02115, USA
Ferrari, Sergio, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy
Fischinger, Peter J., National Cancer Institute, Building 31, Rm. llA56, Bethesda, MD 20014, USA
Freudenstein, Christa, Institut fuer Virusforschung, DKFZ, 1m Neuenheimer Feld 280,6900 Heidelberg, Federal Republic of Germany
Galili, Naomi, Hadassah Medical School, Dept. of Virology, Jerusalem, Israel
Gallo, Robert C., National Cancer Institute, Building 37, Rm. 6B04, Bethesda, MD20014, USA
Galton, David A. G., MRC, Leukaemia Unit, Hammersmith Hospital, Ducane Road, London EI20HS, Great Britain
Gershon, Richard K., Yale University, Dept. of Pathology, 310 Cedar Street, New Haven, CT 06510, USA
Gianni, Alessandro M., Instituto di Clinica Medica I, Padiglione Granelli, Via Francesso Sforza 35, 20122 Milano, Italy
Goldblum, Natan, Hadassah Medical School, Dept. of Virology, Jerusalem, Israel
Grandori, Carla, Instituto Suoeriore di Sanita, Laboratorio Pathologia non Infettiva, Viale Regina Elena 299, 00161 Rome, Italy
Greaves, Melvyn F., Imperial Cancer Research Fund Laboratories, Membrane Immunology Laboratory, Lincoln's Inn Fields, London WC2A3PX, Great Britain
Haseltine, William A., Sidney Farber Cancer Institute, The Jimmy Fund, 45 Binney Street, Boston, MA 02115, USA
Hausen, Harald zur, Institut fuer Virologie im Zentrum fuer Hygiene, Hermann-Herder-Strasse 11,7800 Freiburg, Federal Republic of Germany
Hehlmann, Riidiger, Medizinische Poliklinik der Universitaet Muenchen, Pettenkoferstrasse 8 a, 8000 Muenchen 2, Federal Republic of Germany
Heimpel, Hermann, Zentrum fuer Innere Medizin und Kinderheilkunde der Universitaet Ulm, Steinhoevelstrasse 9, 7900 UIm, Federal Republic of Germany
Helm, Klaus von der, Max-v.-Pettenkofer-Institut, Pettenkoferstrasse 9 a, 8000 Muenchen 2, Federal Republic of Germany
Henderson, Edward S., Roswell Park Memorial Institute, Medicine A, 666 EIm Street, Buffalo, NY 14263, USA
Hoelzer, Dieter, Zentrum fuer Innere Medizin und Kinderheilkunde der Universitaet, Steinhoevelstrasse 9, 7900 Ulm, Federal Republic of Germany
HofIbrand, A. Victor, The Royal Free Hospital, Dept. of Haematology, Pond Street, Hampstead, London NW32QG, Great Britain
Hofschneider, Peter-Hans, Max-Planck-Institut fuer Biochemie, 8033 Martinsried, Federal Republic of Germany
Hossfeld, Dieter Kurt, Medizinische Universitaetsklinik, Abteilung Onkologie/Haematologie, Martinistrasse 52, 2000 Hamburg 20
Izaguirre, Carlos, Imperial Cancer Research Fund Latoratories, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain
Jacquemin, Paul, Institut Ludwig de Recherches sur Ie Cancer, Avenue Hippocrate 74, UCL 7459, 1200 Brussels, Belgium
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Jaenisch, Rudolf, Heinrich Pette-Institut fuer experimentelle Virologie und Immunologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
Kaplan, Henry S., Stanford University Medical Center, Dept. of Radiology, Cancer Biology Research Laboratory, Stanford, CA 94305, USA
Kersey, John H., Dept. of Laboratory Medicine, 420 Delaware Street S.E., Box 609 - Mayo, Minneapolis, MN 55455, USA
Kisseljov, Fjodor L., Cancer Research Center, Kashirskoie Shosse 6, Moscow 115478, USSR
Konze-Thomas, Beate, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 5300 Bonn-Bad Godesberg, Federal Republic of Germany
Kramer, Gisela, Clayton Foundation Biochemical Institute, Dept. ofChemistry, Austin, TX 78712, USA
Kurth, Reinhard, Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Spemannstrasse 37-39, 7400 Tuebingen, Federal Republic of Germany
L'Age-Stehr, Johanna, Robert-Koch-Institut des Bundesgesundheitsamtes, Abteilung Virologie, Nordufer 20, 1000 Berlin 65, Federal Republic of Germany
Lane, Mary-Ann, DANA 940A, Laboratory of Molecular Carcinogenesis, Sidney Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
Lau, Barbara, Institut fuer Haematologie, Abteilung Experimentelle Haematologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany
Lennert, Karl, Institut fuer Pathologie, Abteilung Allgemeine Pathologie und Pathologische Anatomie, Hospitalstrasse 42, 2300 Kiel, Federal Republic of Germany
Levy, Jean-Paul, Laboratoire Immunologie et Virologie des Tumeurs, Hopital Cochin, Groupe INSERM U152, 27 Rue du Foubourg Saint-Jacques, 75674 Paris Cedex 14, France
Lister, T. Andrew, Imperial Cancer Research Fund Laboratories, Dept. of Medical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain
Mannweiler, Klaus, Heinrich-Pette-Institut fuer experimentelle Virologie und Immunologie, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
Mertelsmann, Roland, Memorial Sloan-Kettering, Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Metcalf, Donald, The Walter and Eliza Hall Institute of Medical Research, Cance Research Unit, Royal Melbourne Hospital, Victoria 3050, Australia
Milleck, Jiirgen, Zentralinstitut flir Krebsforschung, Lindenberger Weg 80, 1115 Berlin-Buch, German Democratic Republic
Miller, Denis R., Memorial Sloan-Kettering, Cancer Center, 1275 York Avenue,NewYork,NY 10021, USA
Minowada, Jun, Roswell Park Memorial Institute, 666 Elm Street, Buffalo, NY 14263, USA
Mitchison, N. Avrion, University College London, Tumour Immunology Unit, Gower Street, London WC1E6BT, Great Britain
MoIling, Karin, Max-Planck-Institut fuer Molekulare Genetik, Ihnestrasse 63-73, 1000 Berlin 33, Federal Republic of Germany
Moore, Malcolm A. S., Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Munk, Klaus, Institut fuer Virusforschung, DKFZ, 1m Neuenheimer Feld 280,6900 Heidelberg, Federal Republic of Germany
Myers, Richard M., Harvard University, Cambridge, MA 02248, USA Neth, Rolf-Dietmar, Abteilung fuer Klinische Chemie der Medizinischen
Klinik, Universitaetskrankenhaus Eppendorf, Martinistrasse 62, 2000 Hamburg 20, Federal Republic of Germany
Nicola, Nicos A., The Walter and Eliza Hall Institute of Medical Research, Cancer Research Unit, Royal Melbourne Hospital, Victoria 3050, Australia
XIX
Olsson, Inge L., Research Department 2, E-blocket, University Hospital, 221 85 Lund, Sweden
Papamichail, Michai!, Hellenic Anticancer Institut, 171, Alexandras Ave, Athen, Greece
Papas, Takis S., Carcinogenesis Regulation Section, Laboratory of Molecular Oncology, NIH, Building 41, Suite 400, Bethesda, MD 20014, USA
Peschle, Cesare, Instituto Superiore di Sanita, Laboratorio Patologia non Infettiva, Viale Regina Elena 299, 00161 Rome, Italy
Pimentel, Enrique, Instituto de Medicina Experimentas, Apartado Postal 50587, Caracas 1050-A, Venezuela
Pinkel, Donald, Sunny and Isadore Familian Children's Hospital, City of Hope National Medical Center, 1500 East Duarte Road, Duarte, CA 91010, USA
Ploegh, Hidde L., Institut fuer Genetik der Universitaet zu Koeln, Weyertal 121,5000 Koeln 41, Federal Republic of Germany
Pragnell, Ian B., The Beatson Institute for Cancer Research, Wolfson Laboratory for Molecular Pathology, Garscube Estate, Switchback Road, Bearsden, Glasgow G6llBD, Great Britain
Ramot, Bracha, Chaim Sheba Medical Center, Institute of Hematology, Tel Hashomer, Israel
Reitz, Marvin, National Cancer Institute, Building 37, Rm. 6C09, Bethesda, MD20014, USA
Riethmiiller, Gert, Institut fuer Immunologie, Schillerstrasse 42, 8000 Muenchen 2, Federal Republic of Germany
Rigby, Peter W. J., Imperial College of Science and Technology, Dept. of Biochemistry, London SW72AZ, Great Britain
Rodt, Hans, Institut fuer Haematologie, Abteilung Immunologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany
Rohatiner, Ama, Imperial Cancer Research Fund Laboratories, Dept. of Medical Oncology, St. Bartholomew's Hospital, West Smithfield, London EC1A7BE, Great Britain
Sarin, Prem S., National Cancer Institute, Building 37, Rm. 6A09, Bethesda, MD 20014, USA
Sarngadharan, Mangallasserie, National Cancer Institute, Building 37, Rm. 6A09, Bethesda, MD 20014, USA
Schreiber, Hans, La Rabida Children's Hospital and Research Center, Dept. of Pathology, East 65 Street at Lake Michigan, Chicago, IL 60649, USA
Schubert, Johannes, St.-Joseph-Hospital, Wiener Strasse 1, 2050 Bremerhaven, Federal Republic of Germany
Simon, Markus, Max-Planck-Institut fuer Immunbiologie, Stuebeweg 51, 7800 Freiburg-Zaehringen, Federal Republic of Germany
Singer, Jack W., Veterans Administration Medical Center, 4435 Beacon Avenue South, Seattle, W A 98108, USA
Slevin, Maurice, Imperial Cancer Research Fund Laboratories Dept. of Medical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain
Stein, Harald, Institut fuer Pathologie, Abteilung Allgemeine Pathologie und Pathologische Anatomie, Hospitalstrasse 42, 2300 Kiel, Federal Republic of Germany
Tax, Wi!, St. Radboudziekenhuis, Klinik voor Inwendige Ziekten, Afdeling Nephrologie, Geert Grooteplein zuid 8, 6500 HB Nijmegen, The Netherlands
Terhorst, Cox, Sidney Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA
Thomas, E. Donnall, The Fred Hutchinson Cancer Research Center, Division of Oncology, Dept. of Medicine, 1124 Columbia Street, Seattle, W A 98104, USA
Thorpe, Philip E., Imperial Cancer Research Fund Laboratories, Holburn, London WC2A3PX, Great Britain
Torelli, Giuseppe, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy
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Torelli, Umberto, Instituto di Clinica Medica, Policlinico, Via del Pozzo 71, 41100 Modena, Italy
Uchanska-Ziegler, Barbara, Medizinische Klinik, Abteilung Innere Medizin II, Immunologie-Labor, Otfried Mueller-Strasse, 7400 Tuebingen
Vande Woude, George F., NIH, Building 41, Rm. 100, Laboratory of Molecular Oncology, Bethesda, MD 20205, USA
Vondelich, Laida, Imperial Cancer Research Fund Laboratories, Lincoln's Inn Fields, London WC2A3PX, Great Britain
Watt, Suzanne, Dept. of Pathology, Division ofImmunology, Addenbrooke's Hospital, Hills Road, Cambridge CB22QQ, Great Britain
Weinstein, Howard l., Sidney Farber Cancer Institute, Dept. of Pediatric Oncology, 44 Binney Street, Boston, MA 02115, USA
Weiss, Elisabeth, National Institute for Medical Research, The Ridgeway, London NW7IAA, Great Britain
Weiss, Robin A., Institute of Cancer Research, Royal Cancer Center, Chester Beatty Research Institute, Fulham Road, London SW36JB, Great Britain
Wigzell, Hans, Biomedicinska Centrum, Avdelingen foer Immunologi, 75123 Uppsala, Sweden
Wilson, Elaine L., University of Cape Town, Dept. of Clinical Science and Immunology, Observatory, 7925 Cape, South Africa
Wolf, Hans, Max-v.-Pettenkofer-Institut, Arbeitsgruppe Molekulare Virologie, Pettenkoferstrasse 9 a, 8000 Muenchen 2, Federal Republic of Germany
Wong-Staal, Flossie, National Cancer Institute, Building 37, Bethesda, MD 20014, USA
Young, Brian, The Beatson Institute for Cancer Research, Garscube Estate Switchback Road, Bearsden, Glasgow G6IIBD, Great Britain
Zintl, Felix, Universitaets-Kinderklinik, Kochstrasse 2, 6900 lena, German Democratic Republic
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Wilsede Scholarship Holders
(Granted by the Deutsche Krebshilfe, Leukemia Research Fund Great Britain, and the Leukemia Society of America)
Andreesen, Reinhard, Medizinische Universitaetsklinik, Abteilung Innere Medizin I, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany
Augener, Wiprecht, Medizinische Klinik, Haematologische Abteilung, Hufelandstrasse 55, 4300 Essen, Federal Republic of Germany
Becker, Thomas, Kinder-Poliklinik, Feulgenstrasse 12,6300 Giessen, Federal Republic of Germany
Geoffrey, Brown, Dept. of Immunology, Vincent Drive, Birmingham B 1 52TJ, Great Britain
Crockard, Alistair D., Dept. of Haematology, Royal Victoria Hospital, Belfast BTl26BA, Northern Ireland
Dolken, Gottfried, Medizinische Universitaetsklinik, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany
Drexler, H. Gunter, Zentrum fuer Kinderheilkunde der Universitaet Ulm, Prittwitzstrasse 43, 7900 Ulm, Federal Republic of Germany
Erhardt, Johannes, Institut fUr Immunologie, Schillerstrasse 42, 8000 Muenchen 2, Federal Republic of Germany
Flood, Patrick M., Yale University, Dept. of Pathology, Brady Memorial Laboratory, FMB 202,210 Cedar Street, New Haven, CT 06510, USA
Francis, Gillian, The Royal Free Hospital, Dept. of Haematology, Pond Street, London NW32QG, Great Britain
Ganser, Arnold, Abteilung fuer Klinische Physiologie und Arbeitsmedizin, Oberer Eselsberg, 7900 Ulm, Federal Republic of Germany
Gassmann, Winfried, II. Medizinische Klinik, Metzstrasse 53/57, 2300 Kiel, Federal Republic of Germany
Haas, Wolfgang, Pathologisches Institut, Joseph-S.telzmann-Strasse 9, 5000 Koeln 41, Federal Republic of Germany
Haworth, Catherine, Paterson Laboratories, Christie Hospital, Holt Radium Institute, Withington, Manchester M209BX, Great Britain
Heckmayr, Marlene, II. Medizinische Klinik, Abteilung Onkologie und Haematologie, Universitaetskrankenhaus Eppendorf, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
Hiddemann, Wolfgang, Medizinische Klinik Abteilung Innere MedizinA, Westring 3, 4400 Muenster, Federal Republic of Germany
Hirsch, Friedrich, Medizinische Universitaetsklinik, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany
Hofer, Paul, Institut fuer Immunologie, Schillerstrasse 42, 8000 Muenchen 2, Federal Republic of Germany
Jager, Gundula, Institut fuer Haematologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany
Jendis, Jorg, Max-Planck-Institut fuer Biochemie, Abteilung Hofschneider, 8033 Martinsried, Federal Republic of Germany
Kanz, Lothar, Medizinische Universitaetsklinik, Abteilung Haematologie und Onkologie, Hugstetter Strasse 55, 7800 Freiburg, Federal Republic of Germany
XXIII
Mills, K., University College London, Dept. of Haematology, Gower Street, London WCIE6AU, Great Britain
Miiller, Dorith, Institut und Poliklinik fuer Klinische Immunologie und Rheumatologie, Krankenhausstrasse 12, 8520 Erlangen, Federal Republic of Germany
Mullins, Jim, Harward School of Public Health, Dept. of Microbiology, 66 Huntington Avenue, Boston, MA 02115, USA
Murray, Lesley, Imperial Cancer Research Fund Laboratory, Dept. ofMedical Oncology, St. Bartholomew's Hospital, West Smithfield, London ECIA7BE, Great Britain
Nobis, Peter, Abteilung Molekularbiologie, Physiologisch-Chemisches Institut, Grindelallee 117,2000 Hamburg 13, Federal Republic of Germany
Porzsolt, Franz, Zentrum fuer Innere Medizin der Universitaet UIm, Steinhoevelstrasse 9, 7900 VIm, Federal Republic of Germany
Ritter, Jorg, Kinderklinik, Abteilung Haematologie und Onkologie, RobertKoch-Strasse, 4400 Muenster, Federal Republic of Germany
Riibsamen, Helga, Paul-Ehrlich-Institut, Bundesamt fuer Sera und Impfstoffe, Paul-Ehrlich-Strasse 42-44, 6000 Frankfurt, Federal Republic of Germany
Schlimok, Giinter, II. Medizinische Klinik, Langemarckstrasse 11, 8900 Augsburg, Federal Republic of Germany
Scott, Martin, Stanford University Medical Center, Dept. of Radiology, Cancer Biology Research Laboratory, Stanford, CA 94305, USA
Sieber, Gerhard, Universitaetsklinikum Steglitz, Abteilung Innere Medizin, Haematologie und Onkologie, Hindenburgdamm 30, 1000 Berlin 45, Federal Republic of Germany
Sieff, Colin, The Hospital for Sick Children, Dept. of Haematology, Great Ormond Street, London WCIN3JH, Great Britain
Siegert, Wolfgang, Medizinische Klinik III, Klinikum GroBhadern, 8000 Muenchen 70, Federal Republic of Germany
Strobel, Eva-Susanne, Husserlstrasse 4, 7800 Freiburg, Federal Republic of Germany
Stiinkel, Klaus, Institut fliT Haematologie, Abteilung Immunologie, Landwehrstrasse 61, 8000 Muenchen 2, Federal Republic of Germany
Thone, Ingo, Universitaets-Kinderklinik, Martinistrasse 52, 2000 Hamburg 20, Federal Republic of Germany
Welte, Karl, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021, USA
Wilkie, N., The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G6IIBD, Great Britain
Ziegler, Andreas, Medizinische Universitaetsklinik, Labor fuer Immunologe, 7400 Tuebingen, Federal Republic of Germany
Zigler, Hans-Werner L., Institut fUr Immunologie, Schillerstrasse 42, 8000 Muenchen 2, Federal Republic of Germany
Ziemiecki, Andrew, Institut fuer Virologie, Frankfurter Strasse 107, 6300 Giessen, Federal Republic of Germany
XXIV
Preface
Wir fohlen, daB selbst, wenn aile moglichen wissenschaftlichen Fragen beantwortet sind, unsere Lebensprobleme noch garnicht berilhrt sind.
Ludwig Wittgenstein, Tractus logico-philosophicus (1921), Annalen der Naturphilosophie
In June 1982, physicans and scientists met in Wilsede for the fifth time to discuss origin and treatment ofleukemia.
Lectures and discussions of the meeting closely demonstrated that leukemia can be considered a perfect model for the studies of biology and treatment of cancer in general. The Wilsede meeting has confirmed that cancer is a multifactorial disease in which oncogenes, regulator proteins, the immunsystem, viruses and chemical noxes play important roles. The development of new revolutionary techniques in biological research yielded important insights into the causes of leukemia which are also promising to develop new ways for the treatment of cancer patients.
I am indepted to the chairmen organizing a stimulating and exciting program which included all the present and future aspects of human leukemia.
I am grateful to all participating scientists, the supporting stuff and the Wilsede people for making this meeting both scientifically excellent as well as humanly warm and unforgettable. Last but not least, it must be emphazised that the "Verein Naturschutzpark Liineburger Heide" and Dr. h.c. Alfred Toepfer are responsible for saving this peaceful place for us.
Hamburg, February 1982 Rolf Neth
xxv
Coffee break and lectures in "De Emmenhoir'. Middle left, Wellcome of Dr. h.c. Alfred Toepfer Down left, Melvin Greaves starting his Stohlman lecture Down right, Donall Thomas giving his Stohlman lecture
XXVI
Acknowledgment
We should to thank all who made this workshop possible:
Deutsche Forschungsgemeinschaft Deutsche Krebshilfe Freie und Hansestadt Hamburg Hamburgische Wissenschaftliche Stiftung Leukemia Research Fund, Great Britain Leukemia Society of America Paul Martini Stiftung N iedersachsisches Sozialministeri urn Stifterverband fUr die Deutsche Wissenschaft Universitat Hamburg (Hertha-Grober-Stiftung)
For generous hospitality we thank the Stiftung F. V. S. zu Hamburg, the Amerikahaus in Hamburg, the Freie und Hansestadt Hamburg, and the Hamburger Staatsoper
XXIX