18
Operating Manual Serial No. MicroCentrifuge Purchase Date Place of purchase M icro 12

Hanil Scientific Inc. - Hanil Scientific Inc. - Micro 2 1...14. Repairs must be carried out by a technologist authorized by Hanil scientific Inc. 15. According to IEC61010-2-020 maintain

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  • Operating Manual

    Serial No.

    Micro Centrifuge

    Purchase Date

    Place of purchase

    Micro 12

  • www.ihanil.com2/20

    Copyright ⓒ 2017 Hanil Scientific Inc. All rights reserved.

    Contact Us

    If you have any questions, contact Hanil Scientific Inc. or place of purchase.

    [email protected] / [email protected]

  • [email protected] / [email protected] 3

    CONTENTS

    2 . Product description --------------------------6 2.1 Appearance -6

    3 . Installation -------------------------------------8 3.2 Installation -8

    4 .

    Operation

    --------------------------------------12

    4.1 Controller

    -12 4.2

    Setting RPM

    -13

    4.3

    Setting run time -13

    4.4

    Starting/End the centrifugaion -13

    4.5

    Pulse function -13

    5 .

    Maintenance ----------------------------------14

    6.

    Troubleshooting ---------------------------15

    7. Rotor

    and

    accessories ----------------------17

    3.1 Packing inspection -8

    3.3 Connect power -9

    1. General Considerations -----------------------4

    1.1 Safety -41.2 Transport & Storage -41.3 Safety labe -51.4 Electric safety information -5

    2.2 Delivery package -6 2.3 Specifications -7

    3.4 Opening/closing the lid -93.5 Loading and removal of the rotor -103.6 Loading tubes -11

    5.1 Care instructions -14 5.2 Cleaning -14

    6-1 Possible problems -15,16

  • 4 www.ihanil.com

    1. General Considerations

    1. The centrifuge should be installed on flat surface to maintain level.

    2. Check the voltage to be used, before connecting the centrifuge to the power source.

    4. Do not exceed the maximum rated speed of the rotor or buckets or accessories in use.

    6. If the centrifuge is contaminated by toxic or radioactive samples or infectious blood samples, remove contaminants completely

    7. Substances that may generate volatile or explosive vapor can not be centrifuged.

    8. The balancing work of samples shoud be done in advance before operation.

    11. Only centrifuge with rotor and rotor lid firmly tightened.

    12. Do not block vents.

    Do not insert foreign objects into the holes of the device.

    14. Repairs must be carried out by a technologist authorized by Hanil scientific Inc .

    15. According to IEC61010-2-020 maintain a 30cm “clearance envelope” around the centrifuge while the rotor is spinning.

    16. Please contact the place of purchase or Hanil Scientific Inc. ([email protected]/[email protected])

    for

    product

    repairs.

    1.2 Transport & Storage

    1.1 Safety

    Ambient temperature 5℃ ~ 35℃Maximum relative humidity 30% ~ 85%

    Air pressure 500 ~ 1060 hpa

    Follow precautions and all the safety requirements described on this user manual to prevent any damages and failure of equipment and loss of lives.

    Abnormal vibration is caused when the device is installed on an inclined surface.

    3. Only use rotors, parts, and accessories provided by Hanil Scientific Inc.

    Hanil

    Scientific

    Inc.

    is

    not

    responsible

    for

    damages of the device and accidents caused by using parts and accessories not recommended.

    5. Make sure to prepare necessary safety measures before using samples that are toxic or radioactive samples or pathogenic or samples or infectious blood.

    and take needful actions such as ventilation or isolation of centrifuge.

    9. Before operation, rotor and chamber should be dry.

    10. Do not attempt to slow or stop the spinning rotor by hand.

    13.

    Storage TransportAmbient temperature -10℃ ~ 40℃Maximum relative humidity 10% ~ 90%

    Air pressure 500 ~ 1060 hpa

  • [email protected] / [email protected] 5

    1. General Considerations

    1.3 Safety label attached to products

    1.4 Electric safety information

    Electric shock

    1. Use a power cord only provided with the device.

    2. Use sockets with a protective earth conductor and suitable power cord.

    3. Do not use an extension cord.

    4. Do not place anything on the power cable

    5. Do not block vents.

    6. If you have the following emergencies, shut off the power supply and unplug the power cord from outlet

    and

    Do not open lid until rotor stops completely.

    Rotation direction of the rotor.

    contact your place of purchase or Hani l Scientific Inc.

    - Unusual noises or smell from the equipment.- Damage or wear of a power cord.- Breakdown of circuit breaker, fuse or safety device.- If you spill liquid on the equipment.- If the equipment is exposed to water.- If Some of equipment has been damaged.

  • 6 www.ihanil.com

    2. Product description

    2.1 Appearance

    ① Lid : Lid protects the inside of the chamber.②

    Rotor

    locking

    screw:

    Connect

    the

    rotor

    to

    the

    motor shaft.

    Rotor :

    Connected

    to

    the

    motor

    shaft

    for

    centrifugal

    separation

    ④Lid

    sensor

    :

    Detect

    whether

    lid

    is

    cloesd

    Lid

    open

    switch: Press

    the

    button

    to

    open

    to

    the

    lid.

    ⑥Controller

    :

    Keys

    and

    knobs

    for

    operating

    the

    centrifuge.

    ⑦Power

    socket

    :

    Connection

    for

    power

    cord.

    ⑧Power

    switch

    :

    Switch

    for

    switching

    the

    centrifuge

    on/off.

    ⑨Absorbent

    rubber

    :

    Fix

    the

    device

    on

    the

    laboratory

    bench.

    ①Standard offering

    -

    - Micro12 main body (Rotor included : A2.0-12)

    - Power cable (300/500V, 6A)

    - User manual

    ②Optional products [see 7. Rotor and accessories]

    -

    PCR

    strip

    rotor

    (A0.2-32)

    ⑥ ⑤ ⑦

    ⑧ ⑨

    ③④

    2.2 Delivery package

  • [email protected] / [email protected] 7

    2. Product description

    2.3 Specifcations

    * RPM, RCF value is for A2.0-12.

    * This device does not provide automatic recognition of the rotor. So do not exceed 6,000 rpm when using the PCR rotor.

    Max. RPM 13,500 rpm

    Max. RCF 12,032 xg

    Time ≤ 15 min, Pulse

    Acc. time to max. speed ≤18 sec

    Dec. time from max. speed ≤8 sec

    Tube Capacity 1.5/2.0ml x 12

    Power supply AC110/220V, 50/60Hz

    Dimension (W x D x H, mm) 190 x 202 x 129

    Weight (kg) 1.5

    ※ RCF Table

    * Rcf value was calculated based on the bottom of the rotor hole.

    RPMA2.0-12

    RPMA0.2-32

    0.2ml 0.5ml 1.5/2.0ml 0.2 ml Inner line0.2 ml

    Outer line

    3,000 443 545 594 3,000 313 ~ 443 464 ~ 560 6,000 1,231 1,515 1,651 5,000 870 ~ 1,231 1,289 ~ 1,5568,000 3,151 3,877 4,226

    6,000[ Max.]

    1,253 ~ 1,773

    1,857 ~ 2,241 10,000 4,924 6,058 6,603 12,000 7,090 8,724 9,508 13,500 8,973 11,042 12,032

  • 8 www.ihanil.com

    3. Installation

    3.1 Packing inspection

    3.2 Installation

    ▶ Check the delivery for completeness.

    · Check packing conditions very carefully before unpacking. · Contact Hanil Scientific immediately if damages found.· Check the delivery for completeness.· You can get contact details on packing box or back of the manual.

    ▶ Check all parts for damage.

    Installation on hard and flat ground.• Centrifuge should be installed on hard and flat place.• If the centrifuge is installed in an inclined place, the shaft may be bent due to the weight of the rotor.

    Good ventailation.• For air circulation and safety, maintain a 30cm“clearance envelope”around the centrifuge while the rotor is spinning.

    Constant temperature/humidity• Centrifuge equipped with the sensitive electronic software which is fragile with humidity and temperature.• Must avoid direct ray or heater and be put in the ambience of controlled temp. and air.

    Avoid the corrosive gas• Install the centrifuge in a place where corrosive gas is not generated.• Sulfur dioxide gas and chlorine gas may cause corrosion.

    Leveling• The shaft should be put exactly vertical on the hotizontaliy flat ground by the leveling tool.

  • 3. Installation

    3.3 Connect power

    3.4 Opening/ Closing the lid

    · Connect the device to voltage sources which correspond to the electrical requirements on

    When closing the lid, close it completely until it clicks.

    (O) (X)

    · Use sockets with a protective earth conductor and provided power cord.

    the label attached to the device.

    1. Connect the power cable to the power socket on the right side of the product and plug the cord into the outlet.

    2. Switch the centrifuge on using the power switch on the right side of the device.

    ▶ It is normal product to hear a beep when power is connected.

    the lid-1. Press the 'lid open' switch.-Opening -Closing the lid-

    1. Put the lid on and press the edge of the lid

    If the lid is not completely closed, the device will not operate.

    [email protected] / [email protected] 9

  • 10 www.ihanil.com

    3. Installation

    3.5 Loading and removal of the rotor

    Motor Hub(Motor Shaft)

    · This device is equipped with a rotor .· Only use rotors, parts, and accessories provided by Hanil Scientific Inc . · Do not use a defective rotor .

    1 .

    Using

    a

    flat

    head

    screwdriver,

    turn

    the

    rotor

    locking

    screw

    counterclockwise

    and

    pull

    it

    completely

    out

    with

    the

    washer

    under

    the

    screw.

    2.

    Lift

    up

    the

    rotor

    from

    the

    motor

    shaft .

    3.Load

    the

    rotor

    into

    the

    motor

    shaft .

    4.

    Align

    the

    washer

    with

    the

    hole

    at

    the

    top

    of

    the

    rotor .

    5.

    Insert

    the

    rotor

    lock

    screw

    into

    the

    screw

    hole

    in

    the

    center

    of

    the

    rotor

    and

    turn

    it

    clockwise

    using

    a

    flat

    head

    screwdriver

    to

    completely

    lock

    it

    6.

    Check

    that

    the

    rotor

    is

    completely

    locked .

    Flat head screwdriver

    Rotor lock screw

    Washer

    Rotor

    Follow the steps below to remove or replace the rotor.

  • [email protected] / [email protected] 11

    3. Installaion

    3.6 Loading tubes

    Caution

    Wrong arrangement

    · Only use tubes provided or approved by Hanil Scientific Inc . · Always use the same type of tube .· Tubes should be loaded symmetrically .· Do not exceed the maximum rated speed of the tube . · Same volume of sample should be put on tubes . · Check symmetric loading by balncing tubes with scales .

    1. Check the inside of the rotor hole for moisture or foreign matter.

    2. Tubes must be loaded symmetrically.

    Correct arrangement

  • 12 www.ihanil.com

    4.1 Operation panel

    4. Operation

    버튼 설명

    ① RPM setting knob

    ② Time setting knobRotate clockwise or counterclockwise to set the run time.(To use pulse function, set the time to '0' and press the start key.)

    ③ Start key

    ④ Stop key

    ※ When using a A0.2-32(PCR strip rotor) do not exceed 6,000rpm.

    Rotate clockwise or counterclockwise to set the rpm value.

    Start key

    Stop key

  • [email protected] / [email protected] 13

    4.2 Setting RPM

    4.3 Setting run time

    4.4 Starting/End the centrifugation

    4.5 Pulse function

    4. Operation

    Rotate the 'RPM setting knob' clockwise or counterclockwise to set the rpm value. ▶When using a A0.2-32 (PCR strip rotor) do not exceed 6,000 rpm.

    Rotate 'Time setting knob' clockwise or counterclockwise to set the run time.

    ▶0~15 min.

    1. Check the all parameters are correct and the lid is closed.2. Press the 'Start' key (Operation begins with a beep.)

    ▶ If the lid is not completely closed, it will not operate. ▶ When the set time is over, the device is decelerated and stops with beep sound.▶ Press 'Stop' key to abort centrifugation.▶ Set the 'Rpm setting knob' or 'Time setting knob' to '0' to abort centrifugation. ▶ When the lid is opened during operation, it decelerates and stops.

    1. Set the 'Time setting knob' to '0'2. Press the Start key.3. The device decelerates and stops when the set rpm is reached.

    4.2 Setting RPM

  • 14 www.ihanil.com

    5.Maintenance

    5.1 Care instructions

    • The following procedures should be performed regulary.1. Regularly inspect the rotor chamber for check the motor shaft is nomal.2. Rotate the shaft with your hand to make sure it turns smoothly.3. Use the stopwatch to check that the time setting is correct

    5.2 Cleaning

    • Oustside of the device

    1. Clean the outside of equipment with a soft and dry cloth. ▶ If the device is contaminated, use a mild cleaning fluid to clean.2. Do not use aggressive chemicals on the device such as alchol, benzene, acetone or phenol. 3. Use the stopwatch to check that the time setting is correct.4. Make sure do not scratch the surface of equipment when cleaning it. ▶ Do not use a metal sponge. ▶ If the device is rusted, remove it with a mild detergent and wipe it with a dry cloth.

    • Chamber

    1. If the rotor chamber is not dry, wipe moisture from the chamber with a dry cloth 2. If the rotor chamber is dirty, wipe moisture from the chamber with a dry cloth

    • Rotor

    1. To prevent corrosion, take out the rotor from the rotor chamber.2. If any sample is spilt inside the rotor, wash and dry the rotor well

    • Disposal

    In case of product is to be disposed of, the local wastes laws and regulations ard to be observed.

  • [email protected] / [email protected] 15

    증상 확인 사항

    전원이 들어오지 않아요. [3.3 전원연결]을 참조하여 전원플러그가 빠져 있는지 확인해주십시오.

    동작되지 않아요.Lid가 닫혀지지 않을 경우 동작되지 않습니다. [3.4 Lid 열기]를 참조하여 lid상태를 확인하고 lid를 잘 닫아주십시오.

    Lid가 열리지 않아요.Lid 개방 스위치를 눌러 lid를 열 수 있습니다. Lid 개방 스위치가 파손되었다면 라벡스 기술지원팀으로 문의하여 주십시오.

    Lid가 닫히지 않아요.Lid 걸쇠 부위가 파손되었는 지 확인하십시오. 파손되었다면 라벡스 기술지원팀으로 문의해 주십시오.

    작동 중에 진동과 소음이 나타나요.

    본체 설치 위치가 불안정하면 본체의 수평과 고정여부를 확인하고, 평평한 곳에 수평을 맞춰 다시 설치하십시오.

    로터 장착상태가 불량한 경우 로터 탈착 후 로터 외관을 확인하고 파손된 부위가 있다면 로터 사용을 즉시 중지하십시오.또한 장착 방법이 잘못되었다면 [3.5 로터 장착과 분리]를 참조하여 로터를 정확하게 장착하십시오.

    튜브 삽입이 비대칭이거나 무게가 맞지 않는 경우에는 [3.6 튜브 장착]을 참조하여 튜브 무게를 확인하고 대칭적으로 삽입하십시오.

    6.Troubleshooting

    6.1 Possible problems

    Please check the following information, if the device is faulty.

  • 16 www.ihanil.com

    Lid sensor replacement may be required.

    Need to repair the dial or knob.

    Main body or lid repair may be required.

    6. Troubleshooting

    6.2 Possible problems

    1. The device does not operate.2. The device stops during opertaion.3. There is no beep sound when power is connected.

    Need to repair the controller.Please contact your dealer.

    1. It operates when the lid is open.2. When press the 'start' key, only beeps .

    1. 'Rpm/Time knob' dose not rotate.2. 'Start/stop' key does not press.

    1. The lid does not close.2. The lid does not open.

    Problem Recommended Action

  • [email protected] / [email protected] 17

    7. Rotor and accessories

    12 x 1.5/2.0 ml 45o

    Hole diameter (mm) : 11.5Max. height for tube fit (mm) : 48

    Fixed Angle Rotor, A2.0-12

    Tube

    Tube capacity 0.2 ml 0.5 ml 1.5/2.0 ml

    Adaptor None

    Cat. No. -Adaptor bore (Фx L, mm) 6 x 21 8 x 37 -

    Radius (mm) 44 59 59Max. RPM 13,500Max. RCF 8,965 12,032

    Tube

    Tube capacity (ml) 0.2 8-tube PCR strip

    Radius (mm) Inner line : 31.13 ~ 44.06Outer line : 46.13 ~ 55.68Max. RPM 6,000Max. RCF (g-force) Inner line : 1,773 / Outer line : 2,241

    4 x 8-tube PCR strips, 32 x 0.2 ml 45o

    Hole diameter (mm) : 6.5

    PCR Rotor, A0.2-32

    TR0.2 TR0.5

    12,032

  • Hanil Scientific Inc.16 Arayukro, Gimpo 10136, Rep. of KOREAT. [email protected]