Custom Cosmeceutical Formulations Utilizing Medical Image Analysis and

Biometric Markers

Robert P. ManzoSkinprint

1997 Rt 17MGoshen, New York 10921

845-294-8501www.skinprint.com

� Mass Customization and Personalization is now common� Automobiles� Clothing� Shoes

� Cosmeceutical Technology & Diagnostics Have Converged� Skin diagnostics have become more user friendly� Medical imaging has become more accessible� Cosmetic active ingredients are efficacious� Consumers are more educated� Demographics are creating demand

Background

3 Types of Pigmentation

.

A specific combination of biometric

measurements and imaging will yield

optimal results in most cases

Imaging

� High resolution macro photography � Color ultraviolet reflectance

photography

Epidermal Biometrics

� Hydration� pH� Elasticity & Firmness� TEWL (Barrier)� Sebum� Melanin and redness

Customized/Personalized Cosmeceutical Formulation

� Wrinkles� Color tone� Stratum corneum texture� Hyper pigmentation root cause

� Oxidative damage� Melasma� Post-inflammatory hyper pigmentation� Melanin banding patterns

� Pore size� Bacterial presence (UV)� Determination of epidermal vs. dermal Melasma (UV)� Product occlusion� Lesion count

Imaging Quantitative Markers

Confidential

Subject with Melasma and minor oxidative damage (sun damage)

Confidential

Subject with Melasma and minor oxidative damage (sun damage) and melanin banding

Bacterial presence

Bacterial presence

Confidential

Product Occlusion

� Hydration� Dose and type of moisturizing ingredient

� pH� Bacterial proliferation (acne root cause)� Too many chemical peels (barrier function impairment)

� Elasticity & Firmness� Type and dose of collagen and elastin initiators� Dose of MMP suppressors

� TEWL (Barrier)� Humectant or occlusive moisturizer� Acne initiator

� Sebum� Acne root cause

� Melanin and redness� Baseline for in office procedures (lasers, peels, etc.)� Rosacea quantification� Healing rates

Epidermal Biometrics

Case Study

� 46 year old subject

� Self described issues� “spots” on face� Under eye darkness and puffiness� Chronic dryness� Skin laxicity

� Medical History� Healthy, non-remarkable� Pre-menopausal� 12 year use of Retin-A

Visible and UVCR Analysis

Subject Biometrics Before Treatment

•Hydration 38.0 >62•pH 5.9 4.5 - 5.5•Elasticity & Firmness 47.0 >60•TEWL (Barrier) 9.0 <3•Sebum 3.0 15 - 25•Melanin 15 (spots = 36) 7 (spots = 22)•redness 46 17

Reading Optimal Range

� Cleanser: anionic surfactant base for thorough cleansing

� 3 dimensional bio-peptide approach� Collagen I & III initiator� Fibril bundling strengthener� Matrix Metalloproteinase inhibitor

� Two phase eye treatment approach� Capillary seal� Chelant for removal of iron (hemoglobin)

� Three phase lightening approach� MSH suppressor� Tyramine competition� Tyrosinase inhibitor

� Treatment Lotion� Liposomal ursolic acid (barrier repair)� Occlusive and humectant moisturizers

Treatment Course

Confidential

Results

� Hydration 38.0 67.0� pH 5.9 5.3� Elasticity & Firmness 47.0 56.0� TEWL (Barrier) 9.0 2.0� Sebum 3.0 4*� Melanin 15 (spots = 36) 8 (spots = 25)� redness 46 17

Before After

Results

Results

1997 RT 17M

Goshen, New York 10924

845-294-8501

www.skinprint.com

Robert Manzo