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Higher Content Yeast Imaging and Data Processing
Richard Alexander
Stowers Microscopy Center
May 19, 2009
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
Control Plate Experimental Plate
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
High Content Imaging Candidates
• Reproducible
• Segmentable
• Automatable– Acquisition– Analysis
Use VBA Code to…
• Group Colonies
• Classify Colonies
• Compare between control and experimental plates
Use VBA Code to…
• Group Colonies
• Classify Colonies
• Compare between control and experimental plates
Use VBA Code to…
• Group Colonies
• Classify Colonies
• Compare between control and experimental plates
A B C123
Summary
• Yeast Robot– Pin yeast plate
• Flatbed Scanner– Image yeast plate
• Axiovision– Automate Segmentation– Automate Data Acquisition
• Excel– Automate Data Processing
Acknowledgements
• Rong Li– Das Arupratan– Brian Slaughter
• Molecular Biology– Dan Bradford– Brian Sanderson
