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Reagents

Histology Reagents Product Catalog

Single Source For Your Histology Reagents. Protecting Every Life Story In Your Lab.

Histology Innovation for a NEW Generation

Each tissue specimen your lab processes has a life story behind it. Your

mission is to ensure all your specimens receive optimal processing in

order to deliver the best possible care. Our product line of histological

reagents, dyes and ready-to-use stains enable your lab to deliver increased

productivity, advanced specimen safety and provide highly accurate

processing and staining results.

2 geNeRal data HealtHCaRe | histology reagents product guide

Mounting Media

tMd™ Tissue Marking DyesSuperior adherence to tissue that will not fade in solutions & no fixative required

Cat. # Description

tMd-7 For marking margins & multiple biopsies, orange (O), BlacK (BK), Blue (BL), yelloW (Y), red (R), purple (P), green (G) dyes; includes: 2oz. of each dye/ea; 4ea/cs

tMd-(color) individual Bottles of Marking dyes; orange (O), BlacK (BK), Blue (BL), yelloW (Y), red (R), purple (P), green (G); 8oz/ea; 4ea/cs

tFM™ Tissue Freezing MediumReduced curling affords easy specimen pick up & low water content minimizes freeze fractures

Cat. # Description

tFM-5 embedding matrix for frozen sections; clear, red, yelloW, green & Blue colors; includes: 4oz. of each color/ea; 4 ea/cs

tFM-(color) individual Bottles of Freezing Medium; clear (C), red (R), yelloW (Y), green (G), Blue (B) colors; 4oz/ea; 12ea/cs

shurMount™ Mounting MediumCat. # Description

lc shurMount™ liquid cover glass for manual coverslipping, toluene-based, twist spout disposable bottle; 4oz (118ml)/ea, 12ea/cs ea/cs.

lc-a shurMount™ low Viscosity Mounting medium, Xylene based for automated coverslippers; 16oz (471ml)/ea, 4 ea/cs

lc-W shurMount™ Mounting medium, Water based for immuno procedures; 1oz (20ml)/ea, 4 ea/cs

gRossiNg & FRozeN seCtioNiNg

Differential Quik Stain Kit (Modified Giemsa)High quality, rapid turnaround staining kit. Useful for differential assessment of blood smears, detecting H. pylori microorganisms and immediate interpretation of fine needle aspiration biopsies. Kit contains a fixative for air-dried cell suspensions and/or touch preparation slides. Results in as little as 15 seconds!

Cat. # Description

sps-Mg Differential Quik Stain Kit (Modified Giemsa); 500ml/ea

Histology ReageNts PRoduCt guide | geNeRal data HealtHCaRe 3

Cat. # Description

lc shurMount™ liquid cover glass for manual coverslipping, toluene-based, twist spout disposable bottle; 4oz (118ml)/ea, 12ea/cs ea/cs.

lc-a shurMount™ low Viscosity Mounting medium, Xylene based for automated coverslippers; 16oz (471ml)/ea, 4 ea/cs

lc-W shurMount™ Mounting medium, Water based for immuno procedures; 1oz (20ml)/ea, 4 ea/cs

Embedding Paraffin

Cat. # Description

pg paragard™, liquid paraffin repellent for countertops and equipment; 4 oz. pump bottle/ea, 6 ea/cs.

pg-16 paragard™, liquid paraffin repellent for countertops and equipment; 16 oz. spray bottle/ea, 4 ea/cs.

paragard™ Paraffin RepellentYou’ll be amazed at how well paragard™ stops paraffin from sticking to countertops and equipment, while keeping everything looking brand new. Pleasant smelling, non-toxic spray is safe for use on metal, wood and plastic surfaces. Daily cleaning chores will be reduced to a minimum.

shurFreeze™ Cryogen Spray Tissue blocks can now be cooled with environmentally friendly refrigerant that meets federal specifications for reduced ozone depletion

Cat. # Description

FrZ-1 shurFreeze™ Cryogen spray with nozzle, 12 oz. can w/10 oz. fill; Environmentally safe, does not contain dichlorodifluoromethane; 12 ea/cs See MSDS for HAZ-MAT handling requirements

polyFin™ Paraffin WaxA mixture of highly refined paraffins and co-polymers in a convenient wax pellet.

This unique formulation provides optimal tissue support while maintaining exceptional clarity. polyFin™ has been institutionally tested and found to function flawlessly in pressure/vacuum fluid flow processors. Low (55° C) melting point eliminates any tissue distortion caused by excessive heat during processing.

- Minimal compression & cell disruption - Can safely be use in P/V processors - Ideal handling characteristics - Cut < 2 microns without icing

Cat. # Description

pF polyFin™ tissue infiltrating and embedding media consists of pellets of fine paraffin waxes and co-polymer alloys; 8x1 kg/cs (17.6 lbs)

MiCRotoMy / seCtioNiNg

4 geNeRal data HealtHCaRe | histology reagents product guide

Formalin, 10% neutral buffer (phosphate buffer)A widely used fixative for processing tissues in routine histology laboratories.

The Formalin, 10% neutral buffered is for IHC in clinical laboratories for paraffin embedded tissue.

Zinc Formalin Fixative, pH 6.25Excellent morphological preservation of nuclear & cytoplasmic components.

Zinc Formalin Fixative can replace neutral buffered formalin for routine tissue and immunohistochemical procedures. Best used as a primary fixative, minimizing denaturation.

AcetoneFor hardening and dehydrating tissues.

The EM grade Acetone can also be used for the extraction of various principles from animal and plant substances.

Cat. # Description

a-eM16 acetone, eM grade, 99%, 1l/ea; see Msds for haZ-Mat handling requirements under transportation on air shipments; store at 25°c

Cat. # Description

F-10nB1g Formalin, 10% Neutral Buffered, 1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on air shipments; store at 25°c

F-10nB5g Formalin, 10% Neutral Buffered, 5gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on air shipments; store at 25°c

Cat. # Description

F-Z1g Zinc Formalin,1gal/ea. see Msds for haZ-Mat handling requirements under transportation on air shipments; store at 25°c

FixatioN

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Reagent AlcoholsPerfect for virtually all preparing and staining strategies in the lab.

paraClear™ - Xylene SubstituteRemoves alcohol during the final steps of routine tissue processing

paraClear™ Xylene Substitute is miscible with paraffin for infiltration, and it has a higher flash point than xylene, reducing the flammability danger to the laboratory.

Cat. # Description

a-100ra reagent alcohol, 100% – sda 3a, 95%, isopropyl 5%; acs/usp grade; 1gal/ea, 4ea/cs

a-95ra reagent alcohol, 95% – sda 3a, 95%, isopropyl 5%; acs/usp grade; 1gal/ea, 4ea/cs

a-5050ra shurBlend™ reagent alcohol/isopropyl alcohol Blend, 1gal/ea, 4ea/cs

c-X1g Xylene clearing solvent; acs grade; 1gal/ea, 4ea/cs; see Msds for haZ-Mat handling requirements on air shipments

a-i1g isopropanol - 99.99%, isopropyl alcohol; acs/usp grande; 1gal/ea, 4ea/cs; see Msds for haZ-Mat handling requirements on air shipments

Cat. # Description

c-1g paraClear™ , 1gal/ea; see Msds for haZ-Mat handling requirements under transportation on air shipments; store at 25°c

tissue PRoCessiNg

SS-3030-120shurstain™ H&E Slide Stainer

SS-2030-120shurstain™ Linear Stainer

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Gill’s #2, double strength for Histology & CytologyUsed when a stronger or darker nuclear stain is required for cytology or immunohistochemistry (IHC) counterstaining. Gill’s #2 formulation is a double strength mixture, stains darker and more quickly than Gill’s #1. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

Gill’s #3, triple strength for HistologyAppropriate for routine histology staining where a darker nuclear stain is desired. This is a triple strength formula requiring shorter staining times. Formula is very stable and does not require filtering prior to each use. General purpose nuclear stain, progressive type. Used with hematoxylin and eosin staining.

Harris Hematoxylin, Acidified (mercury-free)Used with hematoxylin and eosin staining. Use Harris Hematoxylin for routine histology and cytology. Similar results can be achieved to those of Gill’s Hematoxylin #1, #2 and #3 formulations by varying staining times. General purpose nuclear stain, regressive type.

Scott’s Bluing ReagentGentle bluing reagent formulation for those specimens that may be affected by more harsh agents. Our premixed and ready-to-use Scott’s Bluing Reagent provides rapid bluing and crisp nuclear detail obtained with routine hematoxylin and eosin stains. For use in both cytology and histology.

Lithium BlueLithium Blue is a gentle yet rapid formulation for bluing hematoxylins in only 5 minutes. Lithium Blue is buffered at an alkalinity of pH 8.0. Our premixed and ready-to-use Lithium Blue provides crisp nuclear detail obtained with Gills, Mayer’s and Harris’ Hematoxylin stains. For use in both cytology and histology.

Cat. # Description

h-g21l gills 2 hematoxylin, 1l/ea. see Msds for haZ-Mat handling requirements under transportation on air shipments; store at room temp.

Cat. # Description

r-sB1g scotts Bluing reagent, 1gal/ea. see Msds for haZ-Mat handling requirements under transportation on air shipments; store at room temp.

Cat. # Description

h-haMF-1p Harris Hematoxylin,Acidified,Mercury free, 500ml/ea. See MSDS for HAZ-MAT handling requirements under transportation on air shipments; store at 25°c

Cat. # Description

h-g31-l gills-3 hematoxylin, 1l/ea. see Msds for haZ-Mat handling requirements under transportation on air shipments; store at 25°c

Cat. # Description

l-B1g Lithium Blue,Buffered,8.0ph, 1gal/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air shipments; store at room temp.

RoutiNe staiNs

Eosin Y, 1.0% alcoholic solution, Non-AcidicCytoplasmic counterstain useful in immuno-histochemistry or treatment of tissue sections with hematoxylin. Contains no acetic acid. Ready-to-use with any automated stainer.

Cat. # Description

e-1y1p eosin y, 1%, 16 oz/ea; see Msds for haZ-Mat handling requirements under transportation on air shipments; store at 25° c

e-yp50gr Eosin Y Certified Powder, 50gm/ea. See MSDS for HAZ-MAT handling requirements under transportation on Air shipments; store at 25°c

histology reagents product guide | geNeRal data HealtHCaRe 7

sPeCial staiNs

AFB Ziehl Neelson Kit (Hot Method)Used to identify acid-fast organisms, mainly Mycobacteria. Helpful in diagnosing Mycobacterium tuberculosis since its lipid rich cell wall makes it resistant to Gram stain. Also stains other bacteria like Nocardia. Acid-fast bacilli stain bright red, non-acid fast organisms stain blue or green.

Cat. # Description

sps-Zn aFB Ziehl neelson Kit (hot Method). size: 1 kit (8oz.)

Results: Acid-fast Bacilli: Bright redBackground: Blue

Alcian Blue Pas Stain KitUsed for staining the acetic mucins and acid musoussubstances.

Cat. # Description

sps-aBp alcian Blue pas stain Kit. size: 500ml/ea

Results:Acidic Mucins: BlueNeutral Mucins: MagentaMixtures of above: Blue/purple

Colloidal Iron Stain KitA colloidal feric oxide treatment for the demostration ofacid polysaccharides.

Cat. # Description

sps-ci colloidal iron stain Kit. size: 500ml/ea

Results:Acid Mucopolysaccharides and Sialomucins: deep BlueNuclei: pink-redCytoplasm: pink

Differential Quik Stain Kit (Modified Giemsa)High quality, rapid turnaround staining kit. Useful for differential assessment of blood smears, detecting H. pylori microorganisms and immediate interpretation of fine needle aspiration biopsies. Kit contains a fixative for air-dried cell suspensions and/or touch preparation slides. Results in as little as 10-15 seconds!

Cat. # Description

sps-Mg Differential Quik Stain Kit (Modified Giemsa). Size: 500ml/ea

Results:Erythrocytes: pink/yellowish red; Platelets: dark Blue/Violet/purple granules; Neutrophils: Blue nucleus, pink cytoplasm, purple granules; Eosinophils: Blue nucleus, Blue cytoplasm, red granules; Basophils: dark Blue purple nucleus, purple granules; Monocytes: purple nucleus, light Blue cytoplasm; Bacteria/Microorganisms: deep Blue, vary in shape; Collagen, Cytoplasm & Muscle: Various shades of pink, Blue, orange & yellow

Fontana Masson Stain KitUse in the histological visualiztion of Argentaffin cells. Also meant for use in the visualization of Melanin in paraffin or frozen sections.

Cat. # Description

sps-F Fontana Massons stain Kit. Kit includes: 100ml/ea. ammonium hydroxide and 500ml/ea. of sliver nitrate, gold chloride, and nuclear Fast red in nalgen bottles.

Results:Argentaffin Cells: Black, Melanin: Black, Nuclei: red, Cytoplasm: light pink

8 geNeRal data HealtHCaRe | histology reagents product guide

Gram’s Stain Kit (with Iodine)Used to stain microorganisms from cultures or specimens by the differential Gram method. Separates bacterial species into two large groups, those that retain the primary dye (gram-positive) and those that lose the primary dye and take the color of the secondary dye (gram-negative). The mechanism of gram stain is based on the distinctive chemistry and physical properties of the cell wall.

Masson’s Trichrome Stain KitThis method is used for the detection of collagen fibers in tissues such as skin, heart, etc. on formalin-fixed, paraffin-embedded sections and may be used for frozen sections as well. Collagen fibers stained blue and nuclei stained black.

Periodic Acid Schiff’s (PAS) Stain KitUsed to demonstrate polysaccharides, neutral mucosubstances and basement membranes primarily in tis-sue. The demonstration of glycogen is best represented by a section of liver with a digestion step used as a negative control in the staining. Kit is supplied with Harris Hematoxylin Acidified as the counterstain.

Verhoeff Van Gleson Elastin Stain KitUseful in demonstrating atrophy of elastic tissue in cases of emphysema, thinning and loss of elastic fibers in arteriosclerosis and other vascular diseases. With aging, changes such as splitting and fragmentation occur, these changes are most obvious in the skin which becomes wrinkled and rather loose-fitting.

Prussian Blue Iron Stain Kit (Reaction for the Demostration of Iron)Histochemical reaction used to demonstrate ferric iron and ferritin. This is not a true staining technique. Protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue. 10% NBF or alcohol can be used. Deparaffinize slides through xylene or substitute to remove paraffin from the section and descending grades of alcohol to water just prior to staining.

Cat. # Description

sps-ge Verhoeff Van Gleson Elastin Stain Kit. Size: 1 kit (500ml.)

Results:Elastic Fibers & Nuclei: BlackCollagen: RedOther Tissue Elements: yellow

Cat. # Description

sps-pas Periodic Acid Schiff’s (PAS) Stain Kit. Size: 1 kit (250ml.)

Results:Nuclei: BlackCytoplasm, Muscle, Erythrocytes: RedCollagen: Blue

Cat. # Description

sps-Mts Masson’s Trichrome Stain Kit. Size: 1 kit (250/500ml.)

Results: Glycogen: Red Nuclei - Blue

Cat. # Description

sps-gsi Gram’s Stain Kit (with Iodine). Size: 1 kit (1 gal.)

Results: Gram-positive cells will appear Blue to Purple, while Gram-negative cells will appear Pink to Red.

Cat. # Description

sps-pBi prussian Blue iron stain Kit (reaction for the demostration of iron. size: 1 kit (250ml.)

Results: Iron (hemosiderin): BlueNuclei: RedBackground: Pink

sPeCial staiNs

histology reagents product guide | geNeRal data HealtHCaRe 9

RoutiNe sPeCial staiNs

Depicted below are routine stained tissue, (Bx) processed on the RTPH-360 45 minutes on the RHE (Rapid Heat Exchange) reducing processing by 2.5 - 3 hours.

AFB: Mycobacterium tuberculosis bacteria revealed using acid-fast Ziehl-Neelson stain; Magnified 1000X. Image courtesy of CDC Publich Health Library/Dr. George P. Kubica.

E-329 Modification of Mowrey’s 1958 Colloidal Iron stain (Small Instestine) for Acid Mucopolysaccharides using Newcomers control slide and Nuclear fast red counterstain. Done by Ruth Chabot 4/22/2010

skin 40x LMC skin HE 10x skin HE 40x stomach HE 10x

stomach HE 20x

placenta HE 40x prostate LMC 10x prostate LMC 40x

skin 10x LMC

Prussian Blue Ironplacenta HE 10x

muscle H&E 10x muscleHE 40x PASMasson’s Trichrome

adipose 10xLMC adipose 40xLMC bladderHE 10xAFB Ziehl Neelsen bladderHE 40x

breast 40xLMC breast 40xLMC-2 Colloidal Iron Stain Fontana Masson

Gram10x

breast 10xLMC

stomach HE 40x VVg 10x skinthyroid HE 10x thyroid HE 40x

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Bottles can be purchased pre-filled, or lab personnel can easily refill bottles from bulk reagent supply. Ask your local dealer for availability in your country.

RTP™-360Rapid Tissue Processor

RTPH™-360 (shown here)Rapid Tissue Processor with Rapid Heat Exchange(located behind unit)

RTP™-300Rapid Tissue Processor

RTPH™-300 (shown here)Rapid Tissue Processor with Rapid Heat Exchange

tissue PRoCessoRs

SP-120Spinning Tissue Processor

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