Upload
cenbimo
View
217
Download
2
Tags:
Embed Size (px)
DESCRIPTION
Histosonda Product list and catalog
Citation preview
THE NEW GENERATIONOF DIAGNOSTIC TOOLS
A POWERFUL NEW GENERATION OFDIAGNOSTIC TOOLSThe HistoSondas® are new single stranded DNA probes for chromogenicvisualization of gene expression by in situ hybridization.
The characteristics of these innovative new probes set them apart fromthose already on the market.
The HistoSondas® consist of single stranded DNA fragments whoselengths are between 10 and 30 times greater than commercial oligo probes(the length of a HistoSonda® is between 200 and 1300 nucleotides).
The probe DNA is targeted against RNA expressed by genes of interestand therefore the HistoSondas® are probes for the detection of geneexpression. The HistoSondas® are specifically designed to be used onformalin-fixed and paraffin-embedded tissue sections.
is fasterThe HistoSondas® have an incubation temperature of 62ºC. The elevatedthermal energy generated allows the molecules to vibrate quicker thereforetaking less time to find their target even though they are situated in a veryviscose dextran containing media. The use of 62ºC as the incubationtemperature for these probes therefore permits rapidity. HistoSonda® is theonly commercial probe whose incubation period is just 30-60 minutes.
is more specificDue to their great lengths the HistoSondas® are more specific. It is statisticallyless probable to find shared sequence homology between long sequences.Therefore the chances of the probe annealing with anything other than thetarget sequence are greatly reduced, increasing specificity. The HistoSonda®incubation temperature of 62ºC also permits that only chains withalmost total sequence homology are annealed (an oligo will not anneal at thistemperature).
is more potentThe potency of the HistoSondas® is directly influenced by the number oflabeled uridines in the DNA sequence. As the HistoSondas® have longsequences, the proba-bility of large num-bers of labeleduridines being incor-porated is greatly in-creased, increasingthe potency of thesignal.
IN COMPARISON TO MOSTTRADITIONAL PROBES ...
THE NEW GENERATION OF DIAGNOSTIC TOOLS
is simple to use
The HistoSonda® hybridization procedure has been simplified to themaximum. It only uses one buffer during the entire technique (PBS). The useof formamide is not necessary and post-incubation washes with formamidehave therefore been suppressed. The hybridization procedure can be carriedout without needing to have specific knowledge of Molecular Biology.Complicated apparatus is not necessary and revealing the probe can beperformed automatically using any immunohistochemistry apparatus.
is easy to transport and store:
The Histosondas® are supplied Lyophilized in single use tubes. Transport andstorage can be carried out at room temperature and they have a long storagelife. Freezers, refrigerators or any special type of care are not required.
AII of the fundamental characteristics describedabove make the HISTOSONDAS unique andpowerful diagnostic tools.Visual example:
THE NEW GENERATION OF DIAGNOSTICTOOLS
Reference: CEM-0001
EBER
> For use in In Vitro Diagnosis> Useful in the detection of cells infected by the
Epstein-Barr virus, both benign and malignant> Fundamentally useful in the diagnosis of
infectious mononucleosis, lymphoid andnon-lymphoid tumors associated withEpstein-Barr viral infection.
INTENDED USE
HIGH QUALITY PRODUCT
HISTOSONDA® EberLymph node, Metastasis of an Undiffe-rentiated Nasopharyngeal Carcinoma
HISTOSONDA® EberLymph node, Hodgkin’s Disease,mixed cellularity
Reference: CEM-0002
HEMOGLOBIN ALPHA CHAIN
> For use in In Vitro Diagnosis> Useful for the detection of erythroid
precursors avoiding the diffuse hematitestaining observed when using Glycophorin A
> Particularly useful in the diagnosis ofmyelodysplastic syndromes and in thedetection of extramedullary erythropoiesis
INTENDED USE
HIGH QUALITY PRODUCT
HISTOSONDA® Hemoglobin ACBone Marrow, Erythroid series
HISTOSONDA® Hemoglobin ACBone Marrow, Myelodysplasticsyndrome
INCUBATES IN 60 MINUTES
Reference: CEM-0003
SERUM ALBUMIN
> For use in In Vitro Diagnosis> Anti-hepatic antibodies are not very specific> The detection of serum albumin RNA identifies
a cell as a hepatocyte whether it is tumoral orbegnin. This probe is particularly useful in thediagnosis of hepatocarcinomas and theirmetastases
INTENDED USE
UNIQUE PRODUCT IN THE MARKET
HISTOSONDA® SERUM ALBUMINHepatocarcinoma
HISTOSONDA® Serum AlbuminGum. Gingival metastasis of ahepatocarcinoma
Reference: CEM-0020
c-erbB2
> For use in In Vitro Diagnosis> The status of c-erbB2 in breast tumors has
been studied until now through the mediumof antibodies, or FISH to visualize geneticamplification. HistoSonda c-erbB2 directlydetects the RNA of this oncoproteinchromogenically, rapidly and simply, offeringgreatly superior results to the aforementionedtechniques
INTENDED USE
UNIQUE PRODUCT IN THE MARKET
HISTOSONDA® c-erbB2c-erbB2 Infiltrating Ductal Carcinomaof the breast
HISTOSONDA® c-erbB2c-erbB2 Infiltrating Ductal Carcinomaof the breast
USES ONLY ONE BUFFER
Reference: CEM-0004
IMMUNOGLOBULIN LIGHT CHAINSKAPPA L5CHAIN
> For use in In Vitro Diagnosis> The Histosondas of the Immunoglobulin
Light Chains are useful for the study ofmonoclonality in lymphoid tumors,lymphoproliferative syndromes, myelomasand for the study of immunodeficiencyassociated lymphoproliferative syndromes
INTENDED USE
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT HISTOSONDA® Kappa L-ChainReactive lymph node
HISTOSONDA® Lambda L-ChainReactive lymph node
Reference: CEM-0005
LAMBDA L5CHAIN
DUAL KAPPA-LAMBDA
> For use in In Vitro Diagnosis> Histosonda Dual Kappa-Lambda is useful
for the study of monoclonality in lymphoidtumors, lymphoproliferative syndromes,myelomas and for the study ofimmunodeficiency associatedlymphoproliferative syndromes
> This probe has the advantage of showingthe two light chains in only one histologicalsection
Reference: CEM-0027
INTENDED USE
HIGH QUALITY PRODUCT
HISTOSONDA® Kappa-LambdaReactive lymph node
HISTOSONDA® Kappa-LambdaReactive lymph node
CAN BE STORED AT ROOMTEMPERATURE
Reference: CEM-0011
INSULIN
> For use in In Vitro Diagnosis> Histosonda Insulin is useful for the study and
classification of neuroendocrine tumors of thepancreas and digestive apparatus and theirmetastasis even in an absence of a clinicallyevident Endocrine Syndrome
INTENDED USE
HISTOSONDA® InsulinNon-tumoral Pancreas
HISTOSONDA® InsulinNon-tumoral Pancreas
Reference: CEM-0018
CYTOMEGALOVIRUS (CMV)
> Research only RUO> Histosonda Cytomegalovirus is intended for
the detection of cells infected by CMV in anylocation found including: lymph node, centralnervous system, retina, lung and intestine.This detection is carried out by in situhybridization in paraffin-embedded,formalin-fixed histological sections
INTENDED USE
HIGH QUALITY PRODUCT
HISTOSONDA® CMVLung, CMV Interstitial Pneumonitis
HISTOSONDA® CMVLung, CMV Interstitial Pneumonitis
HIGH QUALITY PRODUCT
NO FORMAMIDE WASHES
HISTOSONDA® Alpha HCNormal Appendix
Reference: CEM-0006ALPHA Heavy Chain
HISTOSONDA® Delta HCTonsil
Reference: CEM-0007DELTA Heavy Chain
HISTOSONDA® Epsilon HCReactive lymph node
Reference: CEM-0008EPSILON Heavy Chain
HISTOSONDA® Gamma HCTonsil
Reference: CEM-0009GAMMA Heavy Chain
HISTOSONDA® Mu HCTonsil
Reference: CEM-0010MU Heavy Chain
UNIQUE PRODUCTIN THE MARKET
UNIQUE PRODUCTIN THE MARKET
UNIQUE PRODUCTIN THE MARKET
UNIQUE PRODUCTIN THE MARKET
UNIQUE PRODUCTIN THE MARKET
For use in In Vitro DiagnosisThe Histosondas of the ImmunoglobulinHeavy Chains are useful for the study ofmonoclonality in lymphoid tumours,lymphoproliferative syndromes, myelomasand for the study of immunodeficiencysyndromes where the chain-specificplasmatic cells are absent or diminished(supposedly one in every 350 Europeanssuffer from selective deficit of IgA).
INTENDED USE
IMMUNOGLOBULIN HEAVY CHAINS
NO BACKGROUND
Reference: CEM-0013
SOMATOSTATIN
> For use in In Vitro Diagnosis> Histosonda Somatostatin is useful for the
study and classification of neuroendocrinetumors of the pancreas and digestiveapparatus and their metastasis even in anabsence of a clinically evident EndocrineSyndrome
INTENDED USE
HISTOSONDA® SomatostatinNon-tumoral pancreas
HISTOSONDA® SomatostatinNon-tumoral pancreas
Reference: CEM-00014
GASTRIN
> For use in In Vitro Diagnosis> Histosonda Gastrin is useful for the diagnosis
of neuroendocrine tumors producing gastrinand validation of G cell hyperplasias in thestomach
INTENDED USE
HISTOSONDA® GastrinGastric body mucosa
HISTOSONDA® GastrinG-Cell Hyperplasia
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
BRINGS VERY CLEAR IMAGES
Reference: CEM-0015
VASOACTIVE INTESTINAL PEPTIDE (VIP)
> For use in In Vitro Diagnosis> Histosonda Vasoactive Intestinal Peptide is
useful for the study and classification ofneuroendocrine tumors of the pancreas anddigestive apparatus and their metastaseseven in an absence of a clinically evidentEndocrine Syndrome
INTENDED USE
HISTOSONDA® VIPGastric parietal neurons
HISTOSONDA® VIPGastric parietal neurons
Reference: CEM-0016
THYROGLOBULIN
> For use in In Vitro Diagnosis> Histosonda Thyroglobulin is useful for the
study and diagnosis of thyroid tumors andtheir metastases
> Diagnosis of follicular cell thyroid tumors andtheir metastases
INTENDED USE
HISTOSONDA® T-GlobulinNon-tumoral thyroid
HISTOSONDA® T-GlobulinNon-tumoral thyroid
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
TARGETS MESSENGER RNA
Reference: CEM-0017
CALCITONIN
> For use in In Vitro Diagnosis> Histosonda Calcitonin is useful for the
diagnosis of Thyroid Medullary Carcinomasand C cell Hyperplasia
INTENDED USE
HISTOSONDA® CalcitoninThyroid, Medullary carcinoma
HISTOSONDA® CalcitoninThyroid, Medullary carcinoma
Reference: CEM-0012
GLUCAGON
> For use in In Vitro Diagnosis> Histosonda Glucagon is useful for the study
and classification of neuroendocrine tumorsof the pancreas and digestive apparatus andtheir metastasis even in an absence of aclinically evident Endocrine Syndrome
INTENDED USE
HISTOSONDA® GlucagonNon-tumoral pancreas
HISTOSONDA® GlucagonNon-tumoral pancreas
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
MUCH LONGER THAN OLIGOS
Reference: CEM-0019
PANCREATIC PEPTIDE (PP)
> For use in In Vitro Diagnosis> Histosonda Pancreatic Peptide is useful for
the study of pancreatic and extrapancreaticneuroendocrine tumors and their metastases
INTENDED USE
HISTOSONDA® PPNon-tumoral pancreas
HISTOSONDA® PPNon-tumoral pancreas
Reference: CEM-0026
NEUROTENSIN
> For use in In Vitro Diagnosis> Useful in the study of mainly intestinal
neuroendocrine tumors
INTENDED USE
HISTOSONDA® NTSNon-Tumoral Intestinal Mucosa of thesmall intestine
HISTOSONDA® NTSNon-Tumoral Intestinal Mucosa of thesmall intestine.
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
IS FASTER THAN ANY PROBE IN THE MARKET
Reference: CEM-0020
GHRL (Ghrelin/Obestatin)
> For use in In Vitro Diagnosis> Histosonda GHRL is useful for the detection
of cells producing this hormone and theirtumors
INTENDED USE
HISTOSONDA® GHRLGastric body mucosaGHRL-cell hyperplasia
HISTOSONDA® GHRLGastric body mucosaGHRL-cell hyperplasia
Reference: CEM-0022
LYSOZYME
> For use in In Vitro Diagnosis> Histosonda Lysozyme is useful for the
typification and study of histiocytic cells(especially when they are activated andfunctioning) and the detection of monocytesin the bone marrow.
INTENDED USE
HISTOSONDA® LysozymeNormal Bone Marrow
HISTOSONDA® LysozymeBone Marrow: Chronic MyelomonocyticLeukemia
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
IS ALMOST 100 % SPECIFIC
Reference: CEM-0023
ALPHA FETAL PROTEIN (AFP)
> For use in In Vitro Diagnosis> Histosonda AFP is useful for the diagnosis of
primitive liver tumors and embryonic tumorsof the gonads
INTENDED USE
HISTOSONDA® AFPTesticle. Embryonic testicular tumor
Reference: CEM-0025
CHORIONIC GONADOTROPIN SUBUNIT β (CGB)
> For use in In Vitro Diagnosis> Histosonda CGB is useful for the detection and
localization of cells that produce this hormoneas much in choriocarcinomas as in germinalgonad and midline tumors
INTENDED USE
HISTOSONDA® GonadotropinNormal placenta
Reference: CEM-0024
HUMAN 18s RIBOSOMAL RNA (18s RNA)
> For use in In Vitro Diagnosis> Histosonda 18s RNA is useful to asses the
suitability of tissues for hybridization and toasses the quality of RNA that remains in thetissues.
INTENDED USE
HISTOSONDA® RNATonsil
HIGH QUALITY PRODUCT
HIGH QUALITY PRODUCT
Cenbimo S.L.Centro de Biología MolecularC/ Dr. Iglesias Otero s/n27004 Lugo (Spain)
Tel. +34 - 982 23 08 10Or +34 - 696 26 10 65
Revised
9/09-
VERSIO
NCEN
-V-E2.1
www.histosonda.com