Hort Sci-sample paper.pdf

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101

F -

- ( q) , - .T - -70, - . G-, ,- - , - (Rathore . 2004).

S in vitro -

, (Morini . 1990; Fortuna . 1996;Nacheva . 2002; Vujovi . 2007), (Rui, Cerovi 1987; Muna . 1999; Rui . 2003; Sedlk . 2008) (Yeo, Reed 1995; De Paoli . 2002; Rui . 2008). W , - , / , - -

In vitro sht mltplcatn as nfncd by patd

sbcltng shts cntmpay t tstcks

T. Vujovi1, Dj. Rui1, R. Cerovi2

1Fruit Research Institute, aak, Serbia2Maize Research Institute, Zemun Polje, Belgrade, Serbia

Abstact

Vujovi ., Rui Dj., Cerovi R., 2012. In vitro sht mltplcatn as nfncd by patd sbcltng shts cntmpay t tstcks. H. S. (P), 39: 101107.

In vitro (G 5 G 6), (F J) (P) 10 M S -. S . T x G 6 F J, G 5 , . A G 6 F J, x , .A P , . T f . H, - . Q , q ( ). A , z . T (100%) F J, P G 6 ( 90% ) G 5 (70%).R z .

Kywds: ; ; ; ;

S M E S S, P N. R-20013A, R-31064.

Hort. Sci. (Prague) Vol. 39, 2012, No. 3: 101107


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102

(Grant, Hammat 1999).

G, f in vitro . A - - - x R (Norton, Norton 1986), Potentilla fruticosa (Remphrey . 1993), (Varjda, Var-jda 2001), (Hamad, aha 2008) .H, ( , .). N, (Rubus pubescens R.), Debnath

(2004) x , . S, x , in vitro (Grant, Hammat 1999).

G in vitro ,

f f - .

MATERIAL AND METHODS

Plant matal. F -, .. G 5 G 6(Prunus cerasus Prunus canescens), - F J (Prunus salicina L M-

Prunus spinosa L.) P- (Pyrus communis L.), G(L-U, L., D) S I - ( - ), in vitro .

Establshmnt asptc clt. I - - -

. S z x

2 , z 70% (1 20 ), 12 - 10% (/) (0.4%, /, ), . B 0.30.8 - MS (Murashige, Skoog 1962) 2 / N6-z (BA), 0.5 /

-3- (IBA) 0.1 / (GA

3). T -

: , x x .

Sht mltplcatn as nfncd by pat-

d sbcltng. U -, MS

. S (G 5, G 6 F J) - 1 / BA,0.1 / IBA 0.1 / GA

3. F

P MS 0.5 / BA, 0.1 / - (NAA) 0.1 / GA

3. A

30 / 8 / . TH 5.7 121C, 150 P 20 . S

- (Marino . 1985; Yeo, Reed1995). M , .. - x x . T - x (> 0.5 ) - .

S 100 - 50 , 23 1C 16 - ( ,8.83 W/2).

Rtng and acclmatzatn.S - MS - x. R -: (1) 1 / IBA 0.1 / GA

3; (2)

1 / NAA 0.1 / GA3; (3) 1- -

NAA (500 /) - (HF) .T 28

, . R ,

Vol. 39, 2012, No. 3: 101107 Hort. Sci. (Prague)


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103

, z

.Statstcal analyss. A z

ANOVA, D . D .

RESULTS AND DISCUSSION

T in vitro . T

- z - x (Norton, Norton 1986; Grant,Hammatt 1999; Debnath 2004). I -, , ,

f x - ,

. C x - z 48.1% (FJ) 88.3% (G 6) (F. 1). C , - Hartman Kester (1983), -- - , . A,Muna . (1999) - f - .

H, x x (G 5 P) (G 6 F J), - ,

71.7 b

0b

28.3b

88.3a

0.6b

11c

54.2c

0b

45.8a48.1c

4.9a

46.9a

0

10

20

30

40

50

60

70

80

90

100

Rate of contamination Rate of explant necrosis Rosette initiation

%

Gisela 5 Gisela 6

Pyrodwarf Fereley Jaspi

F. 1. E f M

f

-

D

(P< 0.05)

1. Ef G 5 G 6 MS BA 1, IBA 0.1 GA

30.1 /

S-

G 5 G 6

x

x ()

()

x

x ()

()

1. 2.18* 1.05 0.59 3.00 0.80 0.532. 2.10 0.95 0.57 2.13 1.03 0.59

3. 1.57 0.92 0.60 1.82 0.82 0.63

4. 1.60 1.00 0.60 2.48 0.93 0.64

5. 1.56 1.32 0.61 1.48 0.89 0.62

6. 1.59 1.07 0.59 1.74 1.00 0.67

7. 1.59 1.06 0.58 1.39 1.08 0.83

8. 1.24 1.00 0.65 1.73 1.11 0.61

9. 1.29 0.82 0.54 1.65 1.02 0.78

10. 1.50 1.03 0.58 1.70 0.91 0.65

* D (P< 0.05)

(%)



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x- (F. 1).

D , . T - x G 6 F J, G 5 ( 1 2). A G 6 F J, x , - -. D , - G 6 , F J x - x,

. L, - in vitro - Potentilla fruticosa L. x - x (Rem-phrey . 1993). M ( ) , (Remphrey . 1993). H, x-, x

/ ,

f ( ). A- Hussain . (2007), Sterculia urens Rx. ( ) - (z) , . A,Vardja Varjda (2001) Gerbera - HF .H, Hamad aha (2008) x 30 75 . T x 75 -

. H - 30 15 75 - - (Hamad,aha 2008).

A x P- MS BA 0.5, NAA 0.1 GA

30.1 /, -

( 3). Norton Norton (1986) 6 R.

3. Ef P MS BA 0.5, NAA 0.1 GA

30.1 /

S-

Mx

L x ()

L ()

1. 1.23* 1.51 0.88

2. 1.40 1.13 0.68

3. 1.50 1.31 0.77

4. 1.21 1.55 0.72

5. 1.27 1.63 0.85

6. 1.12 1.32 0.57

7. 1.00 1.81

8. 1.00 1.22

9. 1.05 1.41 0.50

10. 1.14 1.40 0.57

*

D (P< 0.05)

2. Ef F J MS BA 1, IBA 0.1 GA

30.1 /

S-

M

x

L x

()

L

()1. 3.24* 0.89 0.59

2. 1.95 0.90 0.57

3. 1.83 0.91 0.59

4. 2.05 1.02 0.66

5. 1.26 1.15 0.70

6. 2.17 0.97 0.60

7. 1.46 1.05 0.71

8. 2.33 1.13 0.57

9. 1.67 1.03 0.56

10. 1.95 0.94 0.60

* D (P< 0.05)



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105

A , - x , , -

(Norton, Norton 1986).N -

- (F. 2). A q x ,

- ( 1-3). Hamad aha(2008) - (30 45 ). I , Norton Norton (1986)

z - , f x , .

A , F J MS -, IBA 1 GA

30.1 /

( 4, F. 3). G 5 - 1 / IBA NAA(65% 70% ),

IBA ( 4, F. 3).

C /, IBA f NAA

Prunus (Koubouris, Vasilakakis2006). I G 5,

G 6 - IBA ( 4). T (90%) 1- - NAA HF (F. 3). P x (90%, F. 3) 1 / IBA NAA (40%, 4). SPyrus in vitro Reed (1995) IBA IBA -

Pyrus communis .Az - ( 90%) , x- G 5 x (61.8%) z (F. 4). A- Yepes Adwinckle (1994),

in vitro . S, in vitro z - z G 5,

B (Bommineni . 2001).

F. 2. In vitro f : () G 5; () G 6; () F J;() P

F. 3. R : () G 5 IBA 1 GA3

0.1 /; () G 6 HF

1- NAA (500 /); () F J IBA 1 GA3 0.1 /;() P IBA 1 GA3

0.1 /



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106

CONCLUSION

A in vitro -

. I , , - in vitro / . G x , -

, - .

R n c s

Bommineni V.R., Mathews H., Samuel S.B., Kramer

M., Wagner D.R., 2001. A in vitro

. HS,36: 11021106.

De Paoli G., Subir E., Battistini A., 2002.In vitro -

P C, ,

. A H,

596: 463467.

Debnath S.C., 2004. C

(Rubus pubescens R.) in vitro x -

. P G R, 43: 179186.

Fortuna F., Citernesi A.S., Morini S., Vitagliano C.,

Giovannetti M., 1996. I -

z z

.

P, 16: 757763.

Grant N.J., Hammat N., 1999. I

: f q. P-

, 19: 899903.

Hamad A.M., aha R.M., 2008. Ef q -

in vitro - (Ananas comosus L. M.)

f . S H, 117:

329334.

Hartman H.., Kester D.E., 1983. P P,

P P. N J, P-H I.

Hussain .M., Chandrasekhar ., Gopal G.R., 2007.

H q Sterculia urens Rx.

. A J B, 6: 16431649.

Koubouris G.,Vasilakakis M., 2006. I in

vitro B. P C

O C, 85: 173180.

4. P f

G/ % N. R () H ()

G 5/1 65* 3.9 1.82 1.54

G 5/2 70 2.8 3.08 1.31

G 5/3 50 2.2 2.13 1.15

G 6/1 0

G 6/2 35 2.4 1.76 1.47

G 6/3 90 8.1 3.02 1.59

P/1 90 4.4 2.08 1.89

P/2 40 1.1 1.48 1.26

F J/1 100 4.7 3.00 1.45

* - D (P< 0.05); 1 - IBA 1 GA

30.1 /; 2 -

NAA 1 GA3 0.1 /; 3-

1- NAA (500 /) HF

61.8b

100a93.2a 90.9a

0

20

40

60

80

100

Gisela 5 Gisela 6 Fereley Pyrodwarf

Aclimatisation(%)

F. 4. T z f . V f D- (P< 0.05)

J



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107

Marino G., Rosati P, Sagrati F., 1985. S in vitro

Prunus . P C O

C, 5: 7378.

Morini S., Loreti F., Sciutti R., 1990. R

M. S. in vitro . A H-

, 283: 207212.

Muna A.S., Ahmad A.K., Mahmoud K., Abdul-Rahman

K., 1999. In vitro -

. P C O C, 59:

203208.

Murashige ., Skoog F., 1962. A

. P-

P, 15: 473497.

Nacheva L., Ivanova K., Manolov P., Zlatev Z., 2002.

P -

Dz 4 (Prunus cerasifera E.)

. A H, 577: 199205.Norton M.E., Norton C.R., 1986. C -

in vitro

R. P C O

C, 5: 187197.

Rathore J.S., Rathore V., Shekhawat N.S., Singh R.P.,

Liler G., Phulwaria M., Dagla H.R., 2004. M-

. I: Srivastava P.S., Narula A.,

Srivastava S. (), P B M

M. N D, A P: 195205.

Reed B., 1995. SPyrus in vitro

. HS, 30: 12921294.

Remphrey W.R., Palmer C.E., Blouw M.J., 1993. In vitro

-

Potentilla fruticosa. P C O

C, 32: 235240.

Rui ., Cerovi R., 1987. Rz C -

in vitro in vivo (In vitro in vivo

C.). R

F, 12: 7375.

Rui ., Sari M., Cerovi R., ulai Lj., 2003. C-

-

in vitro. B P, 47: 463465.

Rui ., Vujovi ., Milenkovi S., Cerovi R ., Mileti

R., 2008. T z -

in vitro P . A

J C S, 1: 6368.

Sedlk J., Paprtein F., Erbenov M., 2008.In vitro -

P-HL . A

H, 795: 395400.

Varjda R., Varjda ., 2001. T f

-

. P E A S, B E,

50: 2232.

Vujovi ., Rui ., Milenkovi S., 2007. I

, F J,

in vitro .

J P, 41: 7177.

Yeo D.Y., Reed B., 1995. M Pyrus

. HS, 30: 620623.

Yepes L.M., Adwinckle H.S., 1994. M

M , f

. P G R, 15:

5567.

R O 14, 2011

A D 9, 2011

Corresponding author:

atjana Vujovi, P.D., F R I, D F P,K P I N. 9, 32000 , S: + 381 32 221 375, x: + 381 32 221 391, -: @...


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