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HPLC analysis
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Author Topic: HPLC analysis (Read 4551 times)
HPLC analysis on: November 03,2007, 07:18:43 AM
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christinaRegular Member
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Posts: 49
How do I analyze a HPLC result?
The lab tech injected the sample into the
machine and I have the result paper for the
sample injected which was caraway oil. However
I'm not sure what to do with this now. It was
recorded in volts per minutes.
Thank You
Re: HPLC analysis Reply #1 on:November 03, 2007,
10:07:21 AM
invisiblegsRegular Member
Mole Snacks: +0/-1
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Posts: 27Analysis of chromatographic results can depend
on what you want or need to know. At its most
basic, c hromatography is a method used to
separate different chemical species from each
other. The resulting chromatographic trace can
thus indicate how pure your sample is by the
number and height of peaks present. Pure
samples will generally only have one peak.
Without integration of peak areas, most of the
information you can glean from a trace will be
qualitat ive. Another bit of information that you
can take from your sample trace will be the
retention time of your product and the relative
chromatographic parameters such as column type
and size, solvent composition, flow rate, and
even temperature. If you have a chromatogram
of a standardized sample of your product, you
could compare retent ion times. With a set of
standard chromatograms at different
concentrations, you could generate a calibration
curve with the integrated peak heights, and
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