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COSMOSIL πNAP is a reversed phase HPLC column with Naphtylethyl group bonded silica packing material. The naphthylethyl group is composed of two fused aromatic rings and forms strong π-π interactions with unsaturated compounds. This column offers improved separation of compounds such as positional isomers that are difficult to analyze with alkyl group bonded materi-als.
HPLC Column for Structural Isomers
α(Nitrobenzene/Benzene)1.00 1.20 1.40 1.60
Ph
PE-MS
πNAP
π-π Interaction
Ph
SiCH3H3C
πNAP
SiCH3H3C
Comparison with Phenyl Columns COSMOSIL πNAP with two aromatic rings has stronger π-π interactions than phenyl column which has one aromatic ring.
The Interaction between Benzene and Nitrobenzene
SiCH3H3C
PE-MS
Stationary Phase
Ph, PE-MSWeak π-π Interaction
C C
C C
C CH H
HH
H H
πNAPStrong π-π Interaction
C C
C C
C CH
H
H H
C
C CC H
HH
H
Sample
Benzene
Weak
Nitrobenzene
NO2
Strong
Naphtylethyl Group
● Naphthalene bonded stationary phase● Enhanced π-π interactions ● Improved selectivity for structural isomers
High Purity Porous Spherical Silica
5 μmapprox. 120Å
Naphtylethyl Group
Hydrophobic Interaction,
π-π Interactionapprox. 11%
Silica Gel
Average Particle Size
Average Pore Size
Stationary Phase
Main Interaction
Carbon Content
SiH3C CH3
5C18-MS-II(95% Methanol)
πNAP(90% Methanol)
5PE-MS(90% Methanol)
5PE-MS(40% Methanol)
πNAP(50% Methanol)
o- m-
p-
5C18-MS-II(40% Methanol)
5PE-MS(40% Methanol)
o- m-
p-
πNAP(50% Methanol)
N
CH3
C
o-
CH3
CN
m-
CH3
CNp-
● Position Isomers of Phthalonitriles
● Tocopherols
5C18-MS-II(30% Methanol)
Application Data
Column SizeFlow RateTemperatureDetection
4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm
Column SizeFlow RateTemperatureDetection
4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm
CN
CN
CN
CN
CN
CN
O
HOR1
R2
R3
R1
R2
R3
α
Me
Me
Me
β
Me
H
Me
γ
H
Me
Me
δ
H
H
Me
Column SizeFlow RateTemperatureDetectionSample
4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 295 nmTocopherol (α, β, γ, δ)(1.25 μg each)
● Position Isomers of Tolunitriles
Sample o-Tolunitrilem-Tolunitrilep-Tolunitrile
(2.0 μg) (2.0 μg) (1.0 μg)
Sample o-, Phthalonitrilem-, Isophthalonitrilep-, Terephthalonitrile
(0.3 μg) (3.0 μg) (0.15 μg)
o- m- p-
● Vitamin E
5C18-MS-II πNAP Column SizeMobile Phase
Flow RateTemperatureDetectionSample
4.6 mm I.D. x 250 mm5C18-MS-IIπNAP1.0 ml/min30℃UV 295 nm1. δ-Tocotrienol2. γ-Tocotrienol3. β-Tocotrienol4. α-Tocotrienol5. δ-Tocopherol6. γ-Tocopherol7. β-Tocopherol8. α-Tocopherol
: Methanol: Methanol : H2O = 90 : 10
5C18-MS-II πNAP 5PE-MS
5C18-MS-II 5PE-MS πNAP
5C18-MS-II(50% Methanol)
5PE-MS(50% Methanol)
πNAP(60% Methanol)
● Adrenal Cortical Hormones
● Isoflavones
● Berberines
HO
O
OOH
HOHO
O
OOH
HO OHO
O
OOH
1.Prednisolone 2.Hydrocortisone 3.Cortisone
N+MeO
OMe
OMe
OMe
N+MeO
OMe
O
O
Cl- Cl-
1. Palmatine Chloride 2. Berberine Hydrochloride
Column SizeFlow RateTemperatureDetectionSample
Column SizeMobile Phase
Flow RateTemperatureDetection
4.6 mm I.D. x 150 mmA: Acetonitrile : 20 mmol/l Phosphate Buffer(pH2.5) = 10 : 90B: Acetonitrile : 20 mmol/l Phosphate Buffer(pH2.5) = 50 : 50B 0→100%/20 min Linear Gradient1.0 ml/min30℃UV 260 nm
(0.05 μg)(0.05 μg)(0.05 μg)(0.05 μg)(0.075 μg)(0.025 μg)
Column SizeMobile Phase
Flow RateTemperatureDetection
4.6 mm I.D. x 150 mm5C18-MS-II : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 40 : 605PE-MS : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 60 : 40πNAP : Methanol : 20 mmol/l Phosphate Buffer(pH2.5) = 80 : 201.0 ml/min30℃UV 254 nm
Sample1. Daidzin2. Glycitin3. Genistin4. Daidzein5. Glycitein6. Genistein
Sample 1. Palmatine Chloride 2. Berberine Hydrochloride (0.2 μg each)
4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 254 nm1. Prednisolone2. Hydrocortisone3. Cortisone
(0.33 μg)(0.33 μg)(0.33 μg)
● Adrenal Cortical Hormones
5C18-MS-II πNAP
Column SizeMobile Phase
Flow Rate
4.6 mm I.D. x 150 mm5C18-MS-IIπNAP1.0 ml/min
TemperatureDetection
30℃UV 254 nm: Methanol : H2O = 50 : 50
: Methanol : H2O = 60 : 40
Sample 1. Triamcinolone2. Cortisone3. Prednisolone4. Hydrocortisone5. Betamethasone6. Dexamethasone7. Triamcinolone Acetonide8. Cortisone Acetate9. Prednisolone Acetate10. Fluocinolone Acetonide11. Fluorometholone(0.23 μg each)
NACALAI TESQUE, INC.Nijo Karasuma, Nakagyo-kuKyoto 604-0855, JAPANTELFAXWebsiteE-mail
For research use only, not intended for diagnostic or drug use.
1104
: +81-(0)75-251-1730: +81-(0)75-251-1763: http://www.nacalai.com: [email protected]
Further Separation
5C18-MS-II(98% Methanol)
πNAP(90% Methanol)
HO
R R =
2.Stigmasterol
1.Cholesterol
3.Campesterol
4.Sitosterol
COSMOSIL PYE is a packed column with pyrene bonded silica packing material. It has stronger π-π interaction than πNAP since pyrene has four aromatic rings.
5PYE(95% Methanol)
● Sterols
Column SizeFlow RateTemperatureDetectionSample
4.6 mm I.D. x 150 mm1.0 ml/min30℃UV 210 nm1. Cholesterol2. Stigmasterol3. Campesterol4. Sitosterol
Ordering Information
Product Number
08082-71
08087-21
08090-61
08091-51
08094-21
Product Name
COSMOSIL πNAP Guard Column
Column Size
4.6 mm I.D. x 10 mm
10.0 mm I.D. x 20 mm
20.0 mm I.D. x 20 mm
20.0 mm I.D. x 50 mm
28.0 mm I.D. x 50 mm
Other size may be available. Please enquire.
Product Name
COSMOSIL πNAP Packed Column
Column Size
1.0 mm I.D. x 150 mm
1.0 mm I.D. x 250 mm
2.0 mm I.D. x 30 mm
2.0 mm I.D. x 50 mm
2.0 mm I.D. x 100 mm
2.0 mm I.D. x 150 mm
2.0 mm I.D. x 250 mm
3.0 mm I.D. x 150 mm
3.0 mm I.D. x 250 mm
4.6 mm I.D. x 150 mm
4.6 mm I.D. x 250 mm
10.0 mm I.D. x 150 mm
10.0 mm I.D. x 250 mm
20.0 mm I.D. x 150 mm
20.0 mm I.D. x 250 mm
28.0 mm I.D. x 250 mm
Product Number
08076-61
08077-51
08566-41
08567-31
08299-51
08078-41
08079-31
08080-91
08081-81
08085-41
08086-31
08088-11
08089-01
08092-41
08093-31
08095-11
CH3Si
H3C
SiH3C CH3