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If you are not completely satisfied with any of the products featured in this brochure, return within 45 days for a FULL REFUND.
*This guarantee is not valid in India and China.
Customize your Communication with Phenomenex iSelect
Visit www.phenomenex.com/icatreg to learn more:
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Phenomenex | Web: www.phenomenex.com
TrademarksClarity, Gemini, Jupiter, Luna and Strata are registered trademarks of Phenomenex, Inc. Axia, BioSep, Cool-Lock, EZ:faast, FilterSys, Giga, Impact, Inferno, MercuryMS, MultiResidue, Oligo-RP, Oligo-WAX, Onyx, pH-LC, Phenex, PREPLC, QSP, Rezex, SecurityGuard, Strata-X, Synergi, Triophilic, TWIN, TWIN-NX, WAXPLUS, Z-Guard and Zebron are trademarks of Phenomenex, Inc. Alltima is a trademark of Alltech Associates, Inc. Bio-Sil and Aminex are registered trademarks of Bio-Rad Laboratories. CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Cyrolite is a trademark of CY/Ro Industries. HYPERSIL is a registered trademark of Thermo Hypersil-Keystone. OBD is a trademark of Waters Corp. Pursuit is a registered trademark of Varian, Inc. Sugar-Pak is a trademark of Waters Corporation. Santoprene is a trademark of Exxon Mobil Corporation. Supelcogel is a trademark of Supelco, Inc. Symmetry and XTerra are registered trademarks of Waters Corp. J&W is a registered trademark of Agilent. DB-5ht is a trademark of Agilent Technologies. SOURCE is a trademark of GE Healthcare Companies. Stabilwax is a registered trademark of Restek. GE is a trademark of General Electric Company. Teflon is a registered trademark of E.I. du Pont de Nemours and Co. ZORBAX is a registered trademark of Agilent Technologies. Phenomenex Inc. is not affiliated with the above companies.
DisclaimersGemini-NX is patent pending. Onyx is a product based on monolithic technology under license from Merck KGaA, Darmstadt, Germany. The comparative data presented may not be representative for all applications. Subject to Phenomenex Standard Terms & Conditions which may be viewed at www.phenomenex.com/TermsAndConditions.
© 2008 Phenomenex, Inc. All rights reserved.
1
T a b l e o f C o n T e n T s
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GC
SPE
ACCESSORIES
HPLC
PREP/PROCESS
BIOSEPARAtIOnS
AMInO ACID AnALYSIS
FOOD AnD BEVERAGE AnALYSIS
Phenomenex | Web: www.phenomenex.com
HP
LCS
PE
Strata
®A
ccessoriesB
ioSep
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2
Strata Impact Precipitation PlatesPart No. Description Unit/Box PriceStrata Impact Precipitation PlatesCE0-7565 Strata Impact Protein Precipitation, Square Well, Filter Plate, 2 mL 2 Strata Impact Starter Kit for Protein PrecipitationCE0-8201 Strata Impact Protein Precipitation Plate (2 ea) Collection Plate 2 mL
(2 ea) Sealing Mat, Santoprene™ (AH0-8199) (2 ea)ea
Strata® Impact Protein Precipitation Plates • Direct in-plate precipitation for faster analysis • Compatibility with organic solvents for clean, leak-free analysis • Efficient precipitation with no cross-contamination or clogging
Protein Precipitation using Acetonitrile (4:1 Solvent Sample Ratio)
App ID 15587 Precipitation Conditions
Format: Strata Impact Protein Precipitation Plate, 2 mL Part No.: CE0-7565 Dispense: 400 μL Acetonitrile into empty wells Add: 200 μL sample to each well Mix: Sample and organic for 30 seconds Apply: Vacuum to sample, collect and inject filtrate
Analytical Column
HPLC Column: Onyx® Monolithic C18 Dimensions: 100 x 4.6 mm Part No.: CH0-7643 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile Gradient: 10-65 % B in 4 min Flow Rate: 4.0 mL/min Temperature: Ambient Detection: LC/MS ESI+ Peaks: 1. Pseudoephedrine (m/z = 166) 2. Propranolol (m/z = 260) 3. Diltiazem (m/z = 415) 4. Verapamil (m/z = 455)
Protein Precipitation using Acetonitrile (3:1 Solvent Sample Ratio)
App ID 15712 Precipitation Conditions
Format: Strata Impact Protein Precipitation Plate, 2 mL Part No.: CE0-7565 Dispense: 300 μL Acetonitrile into empty wells Add: 100 μL of porcine plasma to each well Mix: For 30 sec or let sample stand for 2 min Apply: Vacuum to sample and collect the filtrate and blow down to dryness under slow stream of nitrogen @ 40 ºC Reconstitute: With 100 μL of mobile phase containing 10 ng of analyte of interest
Analytical Column
HPLC Column: Gemini® 3 μm C18 110 Å Dimensions: 150 x 3.0 mm Part No.: OOF-4439-Y0 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.08 % Formic acid / Acetonitrile Gradient: A/B = 90/10 to 50/50 in 5 min, hold for 2 min Flow Rate: 0.5 mL/min Temperature: Ambient Detection: LC/MS ESI+ Injection: 10 μL of sample Sample: 1. Pindolol 2. Metoprolol
0 1 2 3 min
1
2
34
15712
Blank Pindolol
Sample Pindolol
Blank Metoprolol
Sample Pindolol
0 1 2 3 4 50 1 2 3 4 5 6 min
1
2
SIMPLE & CLEAn PRECIPItAtIOn
No More Spin Down
No More Transfer Steps
No More Long Waiting
Acrylic ChamberManifold Lid (Top)
Strata ImpactPrecipitation Plate
Manifold Base (Bottom)
Collection Plate(Hidden Inside)
*Square well sealing mats compatible with 2 mL Strata Impact plates, Strata and Strata-X 96-well plates, and 96 square well collection plates.
Sealing Mats*Part No. Description Unit PriceAH0-7195 Sealing Mats, Pierceable, 96 Square Well, Ethylene Vinyl Acetate (EVA) 50/pk AH0-8199 Sealing Mats, Pierceable, 96 Square Well, Santoprene™ 100/pk
Collection Plates* and 96-Well Plate Vacuum ManifoldPart No. Description Unit PriceAH0-7284 96-well plate manifold, acrylic eaAH0-7192 350 µL/well 96 Square Well Conical V-bottom Collection Plate 50/pk AH0-7193 1 mL/well 96 Square Well Conical V-bottom Collection Plate 50/pkAH0-7194 2 mL/well 96 Square Well Conical V-bottom Collection Plate 50/pk AH0-7279 1 mL/well 96 Round Well Round Bottom Collection Plate 50/pk
ORDERING INFORMATION
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SP
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HP
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Sample Preparation – SPEStrata™-X* Polymeric Sorbents • Excellent tube-to-tube and well-to-well reproducibility • Simple and fast method development • High recoveries for acidic, basic, polar, and non-polar analytes
Sorbent Functional Group Mode Analyte
Strata-X
Strata-XLReversed Phase Polar
& Non-Polar
Strata-X-C
Strata-XL-C
Reversed Phase & Strong Cation Exchange
Bases
Strata-X-CWReversed Phase & Weak Cation Exchange
Bases (including Quaternary Amines)
Strata-X-AWReversed Phase & Weak Anion Exchange
Acids
N ..
n )
)
n )
)
n )
)
n )
)
O
OH
H+
O- S
O
O
NH NH2
O
N ..
n )
)
n )
)
n )
)
n )
)
O
OH
H+
O- S
O
O
NH NH2
O N ..
n )
)
n )
)
n )
)
n )
)
O
OH
H+
O- S
O
O
NH NH2
O N ..
n )
)
n )
)
n )
)
n )
)
O
OH
H+
O- S
O
O
NH NH2
O
ORDERING INFORMATION
Traditional Tubes 1 mL (100/box) 3 mL (50/box) 6 mL (30/box)
Phase 30 mg 60 mg 200 mg 500 mg 100 mg 200 mg 500 mg
Strata-X 8B-S100-TAK 8B-S100-UBJ 8B-S100-FBJ 8B-S100-HBJ 8B-S100-ECH 8B-S100-FCH 8B-S100-HCH
Strata-X-C 8B-S029-TAK 8B-S029-UBJ 8B-S029-FBJ 8B-S029-HBJ 8B-S029-ECH 8B-S029-FCH 8B-S029-HCH
Strata-X-CW* 8B-S035-TAK 8B-S035-UBJ 8B-S035-FBJ 8B-S035-HBJ 8B-S035-ECH 8B-S035-FCH 8B-S035-HCH
Strata-X-AW 8B-S038-TAK 8B-S038-UBJ 8B-S038-FBJ 8B-S038-HBJ 8B-S038-ECH 8B-S038-FCH 8B-S038-HCH
*Strata-X-CW in 1 g/6 mL is available. Please call Phenomenex for more information.
Strata-X Giga™ Tubes 12 mL (20/box) 20 mL (20/box) 1
Phase 500 mg 1 g 1 g
Strata-X 8B-S100-HDG 8B-S100-JDG 8B-S100-JEG
Strata-X-C 8B-S029-HDG 8B-S029-JDG ––
Strata-X-CW 8B-S035-HDG 8B-S035-JDG 8B-S035-JEG
Strata-X-AW 8B-S038-HDG 8B-S038-JDG ––
High-Throughput 96-Well Plates (2/box)
Phase 5 mg 10 mg 30 mg 60 mg
Strata-X 8E-S100-NGB 8E-S100-AGB 8E-S100-TGB 8E-S100-UGB
Strata-X-C –– 8E-S029-AGB 8E-S029-TGB 8E-S029-UGB
Strata-X-CW –– 8E-S035-AGB 8E-S035-TGB 8E-S035-UGB
Strata-X-AW –– 8E-S038-AGB 8E-S038-TGB 8E-S038-UGB
POLYMERIC SORBEntS FOR SIMPLIFIED SPE
Strata-X Giga™ Tubes12 mL (20/box) 20 mL (20/box) 60 mL (16/box)
Phase 2 g 2 g 5 g 5 g 10 g
Strata-XL 8B-S043-KDG 8B-S043-KEG 8B-S043-LEG 8B-S043-LFF 8B-S043-MFF
Strata-XL-C 8B-S044-KDG 8B-S044-KEG 8B-S044-LEG 8B-S044-LFF 8B-S044-MFF
*Strata-X is patented by Phenomenex, Inc.
Phenomenex | Web: www.phenomenex.com
GC
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top Solutions for Environmental Laboratories
Zebron™ ZB-5MSi • Temperature limits: -60 to 360/370 ˚C (Isothermal/TPGC) • Highly inert - improved peak shape for acidic/basic compounds • MS certified low-bleed levels provide maximum sensitivity • Great results for pesticides, phenols, nitrosamines, and EPA Methods
QUALItY HIGH PERFORMAnCE GC COLUMnS
Alternative to Any 5 %-Phenyl- 95 %-Dimethylpolysiloxane Phase:DB-5 Rtx-5ms MDN-5S HP-5ms Rtx-5Amine HP-5msi Rxi-5ms
Certified
MSeSCequipped
6 8 10 12 14 16 18 20 22 min
1 23
4,5
67
812
9,10,11
13,14,15
161718 19 20
21
2223
24
25
2627
If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G018-11-B.
ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.
App
ID 1
6410 Phenols
1. Phenol 2. 2-Chlorophenol 3. 2-Methylphenol 4. 4-Methylphenol 5. 3-Methylphenol 6. 2,6-Dimethylphenol 7. 2-Nitrophenol 8. 2-Ethylphenol 9. 2,4-Dimethylphenol 10. 3,5-Dimethylphenol 11. 2,5-Dimethylphenol 12. 4-Ethylphenol 13. 3-Ethylphenol 14. 2,4-Dichlorophenol
15. Benzoic Acid 16. 2,3-Dimethylphenol 17. 3,4-Dimethylphenol 18. 2.6-Dichlorophenol 19. 4-Chloro-3-methylphenol 20. 2,4,6-Trichlorophenol 21. 2,4,5-Trichlorophenol 22. 2,4-Dinitrophenol 23. 4-Nitrophenol 24. 2,3,4,6-Tetrachlorophenol 25. 4,6-Dinitro-2-methylphenol 26. Pentachlorophenol 27. Dinoseb
Column: Zebron ZB-5MSi Dimensions: 30 meter x 0.25 mm x 0.25 μm Part No.: 7HG-G018-11 Injection: Split 5:1@ 240 ˚C, 1 μL Carrier Gas: Helium @ 1.2 mL/min (constant flow) Oven Program: 60 ˚C to 140 ˚C @ 5 ˚C/min to 280 ˚C @ 10 ˚C/min Detector: MSD @ 230 ˚C; 45-450 amu Sample:
ID(mm) df(µm) Temp. Limits °C Part No. Price15-Meter I
I I I 0.25 0.25 -60 to 360/370 7EG-G018-11 30-Meter0.25 0.25 -60 to 360/370 7HG-G018-110.25 0.50 -60 to 360/370 7HG-G018-17 0.32 0.25 -60 to 360/370 7HM-G018-11 0.32 0.50 -60 to 360/370 7HM-G018-17 60-Meter0.25 0.25 -60 to 360/370 7KG-G018-11 Test MixZebron ZB-5MSi AGO-8362
ORDERING INFORMATION
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Zebron™ ZB-5Ht Inferno™ • First non-metal column stable to 430 ˚C • Bake off contaminates at extremely high temperatures • Longer column lifetime for high boiling compounds, long-chained hydrocarbons, and surfactants
QUALItY HIGH PERFORMAnCE GC COLUMnS
16144
6 8 10 12 14 16 18 20 min
1
2
34
6
78
910
111213
14
1516
1718
19
5Zebron Inferno at
350 ºCGreat detection of late eluting peak!
2007 R&D 100 Award Recipient
If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G015-11-B.*0.53 mm ID columns are rated to 400 ˚C max operational
ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.
ID(mm) df(µm) Temp. Limits °C Part No. Price15-Meter I
I I I 0.25 0.10 -60 to 400/430 7EG-G015-02 0.32 0.10 -60 to 400/430 7EM-G015-02 0.32 0.25 -60 to 400/430 7EM-G015-11 0.53 0.15 -60 to 400 7EK-G015-05 20-Meter0.18 0.18 -60 to 400/430 7FD-G015-08 30-Meter0.25 0.10 -60 to 400/430 7HG-G015-02 0.25 0.25 -60 to 400/430 7HG-G015-11 0.32 0.10 -60 to 400/430 7HM-G015-02 0.32 0.25 -60 to 400/430 7HM-G015-11 0.53 0.15 -60 to 400 7HK-G015-05 Test MixZebron ZB-5HT AG0-515530-Meter 5 Meter Guardian0.25 0.10 -60 to 400/430 7HG-G015-02-GGA
Alternative to Any 5 %-Phenyl-95 %-Dimethylpolysiloxane High-temperature Phase:DB-5ht VF-5ht HT-5 Stx-5HT XTI-5HT
PolyBrominated Diphenyl Ethers (PBDEs)
Column: Zebron ZB-5HT Inferno
Dimensions: 15 meter x 0.25 mm x 0.10 μm
Part No.: 7EG-G015-02
Injection: On-Column @ 73 ºC, 0.5 μL
Carrier Gas: Helium @ 1.5 mL/min (constant flow)
Oven Program: 70 ºC to 160 ºC @ 25 ºC/min to 350 ºC @ 10 ºC/min for 5 min
Detector: ECD @ 400 ºC
Sample: 1. BDE-25 11. BDE-992. BDE-28 12. BDE-853. BDE-75 13. BDE-1544. BDE-49 14. BDE-1535. BDE-71 15. BDE-1386. BDE-47 16. BDE-1837. BDE-66 17. BDE-1908. BDE-77 18. BDE-2039. BDE-100 19. BDE-209
10. BDE-119
App
ID 1
6144
Note: Sample was 2.5 ppm in iso-octane.
Certified
MSeSCequipped
Outperforms Agilent J&W DB-5htLifetime Comparison†
7.0
7.5
8.0
200 40 60 80
Hours at 400 °C
Ret
entio
n Ti
me
(min
)
Zebron™ ZB-5HT Inferno™
Agilent® J&W® DB-5ht™
Conditions for all columns:
Dimensions: 30 meter x 0.25 mm x 0.10 μm
Injection: 1.0 μL of test mix AG0-7578
Carrier Gas: Constant Flow Helium @ 1.9 mL/min
Oven Program: 120 °C Isothermal
Detector: Flame Ionization Detector (FID) @ 400 °C
Sample: Pentadecane
Longer Lifetime!
Zebron ZB-5HT outperforms Agilent J&W DB-5ht. Columns were held at 400 °C for 2 hours, then the oven was lowered to 120 °C for Pentadecane analysis.† All columns were new, never used, prior to this testing and purchased either directly from the original manufacturer or through an authorized distributor. All testing was carefully controlled to ensure conditions were similar for all columns involved. The comparative data is not representative of every application.
ORDERING INFORMATION
Phenomenex | Web: www.phenomenex.com
GC
Zeb
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SP
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QUALItY HIGH PERFORMAnCE GC COLUMnS
Similar to:MDN-12 DB-XLB Rtx-XLB
top Solutions for Environmental Laboratories
Zebron™ ZB-XLB • Low polarity Si-arylene column • Provides alternate selectivity to standard 5-type phases • Low bleed and sensitivity levels designed for MS detectors • Great for confirmation of pesticides, PCBs, or other environmental samples • Good tool for identifying unknown contaminants
If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G019-11-B.*Thicker films(≥1.0 um df) are rated to 320/340 ˚C (Isothermal/TPGC).
ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.
ID(mm) df(µm) Temp. Limits °C Part No. Price15-Meter I
I I I 0.25 0.25 30 to 340/360 7EG-G019-11 20-Meter0.18 0.18 30 to 340/360 7FD-G019-08 30-Meter0.25 0.25 30 to 340/360 7HG-G019-11 0.25 0.50 30 to 340/360 7HG-G019-17 0.32 0.25 30 to 340/360 7HM-G019-11 0.32 0.50 30 to 340/360 7HM-G019-17 0.53 1.50 30 to 320/340 7HK-G019-28 60-Meter0.25 0.25 30 to 340/360 7KG-G019-11 Test MixZebron ZB-XLB AG0-7578
8 9 10 11 12 13 14 15 16 min
1 2
3
4
56 7
89
10
11
12
13
1415
1617 18
19
20
Certified
MSeSCequipped
App
ID 1
6414 Chlorinated Pesticides: EPA Method 8081A
Column: Zebron ZB-XLB
Dimensions: 30 meter x 0.25 mm x 0.25 μm
Part No.: 7HG-G019-11
Injection: Split 111:1 @ 250 ˚C, 1.5 μL
Carrier Gas: Helium @ 0.9 mL/min (constant flow)
Oven Program: 110 ˚C to 320 ˚C at 15 ˚C and hold until last peak elutes
Detector: ECD @ 350 ˚C
Sample: 1. a-BHC 2. g-BHC 3. b-BHC 4. d-BHC 5. Heptachlor 6. Aldrin 7. Heptachlor epoxide 8. g-Chlordane 9. a-Chlordane 10. Endosulfan I
11. 4,4’-DDE 12. Dieldrin 13. Endrin 14. 4,4’-DDD 15. Endosulfan II 16. Endrin aldehyde 17. 4,4’-DDT 18. Endosulfan sulfate 19. Methoxychlor 20. Endrin ketone
ORDERING INFORMATION
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Zebron™ MultiResidue™ 1 & 2 • Novel phase designed for pesticides, herbicides, and insecticides • Designed for dual-column analysis of pesticide samples • Baseline resolution and confirmation of 20 chlorinated pesticides in < 10 min! • Low bleed performance for pesticide confirmation by MS
QUALItY HIGH PERFORMAnCE GC COLUMnS
43
1
23
4
5
7
6
8 91011
1213
14
15161719
1820
21
2223
6 85 7 9 min
1
2
3
4
5
76
8
9 101112
13 14
151617 19
18
20
21
2223
43 6 85 7 9 10 min
MR-2MR-1
Baseline Resolution in 10 Minutes!
If you need a 5 in. cage, simply add a (-B) after the part number.
ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.
ZB-MR-1ID(mm) df(µm) Temp. Limits °C Part No. Price30-Meter I
I I I 0.25 0.25 -60 to 320/340 7HG-G016-11 0.32 0.50 -60 to 320/340 7HM-G016-17 0.53 0.50 -60 to 320/340 7HK-G016-17
ZB-MR-2ID(mm) df(µm) Temp. Limits °C Part No. Price30-Meter I
I I I 0.25 0.20 -60 to 320/340 7HG-G017-10 0.32 0.25 -60 to 320/340 7HM-G017-11 0.53 0.50 -60 to 320/340 7HK-G017-17
Organochlorine Pesticides - Fast: EPA Method 8081 Part Nos.: 7HM-G016-17 (MR-1) 7HM-G017-11 (MR-2) Injection: Split @ 250 °C, 1 µL Carrier Gas: Helium @ 3.4 mL/min (constant flow) Oven Program: 100 °C for 0.5 min to 220 °C @ 35 °C/min to 340 °C @ 20 °C/min to for 2 min Detector: ECD @ 350 °C Sample: Performed using a 5 m Z-Guard™ Column and a 'Y' splitter.
App
ID 1
6153
, 161
54
1. TCMX (Surr) 2. 1-Bromo-2-Nitrobenzene (IS) 3. a-BHC 4. g-BHC (Lindane) 5. b-BHC 6. d-BHC 7. Heptachlor 8. Aldrin 9. Heptachlor Epoxide 10. trans-Chlordane (gamma) 11. cis-Chlordane (alpha)
12. Endosulfan I 13. 4, 4' -DDE 14. Dieldrin 15. Endrin 16. 4, 4' -DDD 17. Endosulfan II 18. Endrin Aldehyde 19. 4, 4' -DDT 20. Endosulfan Sulfate 21. Methoxychlor 22. Endrin Ketone 23. Decachlorobiphenyl (Surr)
Certified
MSeSCequipped
ORDERING INFORMATION
Phenomenex | Web: www.phenomenex.com
GC
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top Solutions for Pharmaceutical Laboratories
Zebron™ ZB-624 • Great separation of volatile organic flavor and fragrance additives and residual solvents in industrial or pharmaceutical products (OVIs) • Specifically designed for the separation of volatile organic compounds (VOCs) • Formulated for low bleed • Speed up run times and re-equilibration with high temperature limit • Equivalent to USP Phase G43
QUALItY HIGH PERFORMAnCE GC COLUMnS
Alternative to Any 6 %-Cyanopropylphenyl- 94 %-Dimethylpolysiloxane Phase:DB-624 AT-624 DB-VRX Rtx-1301 CP-1301 SPB-624 Rtx-624
BP624 007-502 HP-VOC 007-624 Rtx-VMS DB-1301 SPB-1301
CP-Select 624 CB
ID(mm) df(µm) Temp. Limits °C Part No. Price20-Meter I
I I
I 0.18 1.00 -20 to 260 7FD-G005-22 30-Meter0.25 1.40 -20 to 260 7HG-G005-27 0.32 1.80 -20 to 260 7HM-G005-31 0.53 3.00 -20 to 260 7HK-G005-36 60-Meter0.25 1.40 -20 to 260 7KG-G005-27 0.32 1.80 -20 to 260 7KM-G005-31 0.53 3.00 -20 to 260 7KK-G005-36 75-Meter0.53 3.00 -20 to 260 7LK-G005-36 105-Meter0.53 3.00 -20 to 260 7NK-G005-36 Test MixZebron ZB-624 AG0-5159
Conditions same for all runs
Column: Zebron ZB-624 Dimensions: 30 meter x 0.32 mm x 1.80 µmPart No.: 7HM-G005-31Injection: Split 5:1 @ 140 °C, 1 mLCarrier Gas: Helium @ 35 cm/sec (constant flow)Oven Program: 40 °C for 20 min to 240 °C @ 10 °C/min for 20 minDetector: FID @ 250 °C
USP Method <467> Residual Solvents Procedure A. A) Class 1 for water soluble compounds. B) Class 2 mix A for water soluble compounds. C) Class 2 mixB for water soluble compounds.
A Sample: 1. 1,1-Dichloroethene 2. 1,1,1-Trichloroethane 3. Carbon tetrachloride 4. Benzene 5. 1,2-Dichloroethane
4 6 8 10 12 min
APPID 16348
2
3
1
4
5
B Sample: 1. Methanol 2. Acetonitrile 3. Dichloromethane 4. cis-1,2-Dichloroethene 5. THF 6. Cyclohexane 7. Methylcyclohexane
10 155 20 25 30min
12
3
4
5
6 7
8
9 10
11
12,13
14
APPID 16349
8. 1,4-Dioxane 9. Toluene 10. Chlorobenzene 11. Ethylbenzene 12. m-Xylene 13. p-Xylene 14. o-Xylene
C Sample: 1. Hexane 2. Nitromethane 3. Chloroform 4. 1,2-Dimethoxyethane
5. Trichloroethylene 6. Pyridine 7. Methylbutylketone 8. Tetralin
10 20 30 min
APPID 16350
1
3
2
5
46
7
8
ORDERING INFORMATION
App
ID: 1
6348
App
ID: 1
6349
App
ID: 1
6350
Phenomenex | Web: www.phenomenex.com
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0
1 2 3 4 5 6 7 8 9 10 11 12min
12
3
4
5
6
7
8
9
10
1112 13
16 19
21
22
23
25
26
27
28
29
30, 31
32
3334 36
37
38 39
40
41
42
43
44
4749
50
51
52
53
54
56
57
48
45, 46
3524
17,1814, 15
55
Zebron™ ZB-WAXPLUS™
• 100 % aqueous stable • Extremely inert for acidic compounds • Enhanced selectivity for low boiling solvents • High retention of alcohols and other chlorinated solvents • Solvent rinsible • Equivalent to USP Phase G16
QUALItY HIGH PERFORMAnCE GC COLUMnS
Alternative to Any Polyethylene Glycol Phase:DB-WAX PEG 20M HP-20M Stabilwax®
Supelcowax-10 AT-WAX CP-WAX 52 CB AT-AquaWax
Carbowax-20M Permabond CW 20M CAM BP20
Solvents
Column: Zebron ZB-WAXPLUS Dimensions: 30 meter x 0.25 mm x 0.25 µmPart No.: 7HG-G013-11Injection: Split 50:1 @ 220 °C, 0.2 µLCarrier Gas: Helium @ 1.2 mL/min (constant flow)Oven Program: 30 °C for 1 min to 70 °C @ 14 °C/min to 220 °C @ 25 °C for 3 minDetector: MSD @ 250 °C; 18-350 amuSample: 1. Air
2. Pentane 3. Hexane 4. Ethyl ether 5. 2-Methoxy-2-methyl-2-propane (MTBE) 6. Heptane 7. Cyclohexane 8. 1,1-Dichloroethene 9. Methylcyclohexane 10. Acetone 11. Formic acid ethyl ester (Ethyl formate) 12. Acetic acid methyl ester (Methyl acetate) 13. Tetrahydrofuran (THF) 14. Carbon tetrachloride 15. 1,1,1-Trichloroethane 16. Ethyl acetate 17. Isopropyl acetate 18. 2-Butanone (MEK) 19. 1,2-Dimethoxyethane 20. Dichloromethane 21. Isopropanol 22. Benzene 23. Propyl acetate 24. 1,2-Dichloroethene 25. Trichloroethylene 26. Acetonitrile 27. Methylisobutylketone (MIBK) 28. Isobutyl acetate 29. Chloroform
30. Toluene 31. n-Propanol 32. Water 33. 1,4-Dioxane 34. 1,2-Dichloroethane 35. Butyl acetate 36. 2-Hexanone (MBK) 37. 2-Methylpropanol 38. Ethylbenzene 39. p-Xylene 40. m-Xylene 41. Butanol 42. Nitromethane 43. Cumene 44. 2-Methoxy ethanol 45. Pyridine 46. o-Xylene 47. 3-Methyl butanol 48. Chlorobenzene 49. n-Pentanol 50. DMF 51. Anisole 52. Tetralin 53. DMSO
54. Ethylene glycol (1,2-ethanediol) 55. Methylacetamide 56. n-Methylpyrrolidinone 57. Formamide
ID(mm) df(µm) Temp. Limits °C Part No. Price10-Meter I
I I I 0.10 0.10 20 to 250/260 7CB-G013-02 15-Meter0.25 0.25 20 to 250/260 7EG-G013-11 20-Meter0.18 0.18 20 to 250/260 7FD-G013-08 30-Meter0.25 0.25 20 to 250/260 7HG-G013-11 0.32 0.25 20 to 250/260 7HM-G013-11 0.32 0.50 20 to 250/260 7HM-G013-17 0.53 1.00 20 to 230/240 7HK-G013-22 60-Meter0.25 0.25 20 to 250/260 7KG-G013-11 0.32 0.50 20 to 250/260 7KM-G013-17 0.53 1.00 20 to 230/240 7KK-G013-22 Test MixZebron ZB-WAXPLUS AG0-7869
App
ID 1
5926
If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G018-11-B.
*Thicker films (≥1.0 μm df) are rated to 230/240 ˚C (Isothermal/TPGC).
ProtECt your GC CoLumn. try Z-Guard witH your nExt ZEbron ordEr.
GC/MS analysis of class 1, 2, and 3 solvents
ORDERING INFORMATION
Phenomenex | Web: www.phenomenex.com
GC
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Accesso
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™
10
Phenex™ Syringe Filters • Rapid sample and solvent filtration prior to chromatography • Particulate, PVC, and extractable-free filters • More consistent, reproducible results
Phenex™ SYRInGE FILtERS
Syringe Filter Selection Guide
3. Choose a filter membrane according to the characteristics of your sample and filtering objective Membrane Type Recommended Uses
RC(Regenerated Cellulose)
Hydrophilic Regenerated Cellulose filter membranes are compatible with a very broad range of aqueous and mixed-organic solutions, making them one of the most universal filter materials prior to chromatography. Phenex-RC filters also exhibit fast-flow and ultra-low protein and non-specific binding characteristics. Due to the beneficial material characteristics, Phenex-RC membranes are broadly recommended as an excellent general purpose/high-performance sample filter for most applications.
PTFE Teflon®
(Polytetrafluoroethylene) Phenex-PTFE membranes are inherently hydrophobic and excellent for filtration of 100 % organic or highly acidic or basic samples and solvents. Widely used in chromatography, it is especially well suited for the clarification of non-aqueous samples. Although this membrane is hydrophobic, it can be made hydrophilic by wetting the membrane with alcohol and then flushing with deionized water.
PES(Polyethersulfone)
Polyethersulfone membranes exhibit very fast-flow and ultra-low protein binding characteristics and are ideally suited for use in many life science clarification applications. Phenex-PES membranes typically offer better chemical resistance than cellulose acetate and are broadly recommended for filtering critical biological samples, tissue culture media, additives and buffers.
NY(Nylon)
Nylon has inherent hydrophilic characteristics and works well for filtration of many aqueous and mixed-organic sample types. For applications that require low protein or non-specific binding characteristics, Phenomenex recommends Phenex-RC (Regenerated Cellulose) filters.
CA(Cellulose Acetate)
Cellulose Acetate membranes exhibit ultra-low protein binding and are broadly used in the filtration of biological samples. In combination with a glass pre-filter (Phenex-GF/CA), this membrane is excellent for filtration of tissue culture media, general biological sample filtration and clarification.
GF(Glass Fiber)
Phenex-GF (Glass Fiber) filters are made of inert borosilicate glass and have a nominal 1.2 µm pore size. They are commonly used with highly viscous samples or samples that contain high concentrations of particulate matter (e.g., food analysis, biological samples, soil samples, fermentation broth samples, removal of yeasts, molds, etc.). Glass fiber filters can be used alone or in series with other Phenex filter membranes such as the 0.45 µm pore Phenex-RC filter to reduce clogging of the membrane and optimize flow.
1. Choose filter diameter based on sample volume
2. Choose pore size based on the nature of your sample and chromatographic methodSample Description Recommended Filter Pore Size
General aqueous or mixed organic samples prior to HPLC analysis with column packed with > 3 µm particles.General clarification of GC, SFC, CE, and GPC samples. 0.45 µm
Viscous samples or samples containing high levels of particulate matter.
General aqueous or mixed organic samples prior to HPLC analysis with columns packed with < 3 µm particles. Removal of fine particulate matter prior to GC, SFC, CE, and GPC samples. 0.20 µmGas samples prior to GC. Liquid samples prior to UHPLC or LC/MS. Other particulate-sensitive methods.
Viscous samples such as serum, plasma or other biological matrices. Solutions with high particulate load such as, some environmental or food and beverage applications.
In-line Glass Fiber Pre-Filter or Integrated Glass Fiber Filter Unit
Maximum chemical compatibility 100 % integrity tested Minimum extractables Low hold-up volume Excellent flow rate Low protein adsorption High total throughput Bi-directional use Certified quality
4 mm Diameter I 15 - 17 mm Diameter I 25 - 28 mm Diameter
≤ 2 mL Sample Volume 2 to 10 mL Sample Volume 10 to 100 mL Sample Volume
Phenex Offers:
Phenomenex | Web: www.phenomenex.com Acc
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Phenex™ SYRInGE FILtERS
For high load and particulate-laden samples you may consider placing a Glass Fiber (GF) pre-filter in line with the membrane syringe filter of your choice.
Phenex™ Syringe Filters (cont'd)
Above syringe filters are non-sterile. Housing is made of medical-grade polypropylene (PP). Luer lock inlet/slip outlet connections unless otherwise indicated.
1. 17 mm diameter.2. Glass fiber filters are 28 mm diameter and made of borosilicate. They will remove 90 % of all particles >1.2 µm. 3. Housing material is methacrylate butadiene styrene (MBS) polymerisate. Also known as Cyrolite™.4. Cellulose acetate is surfactant-free.5. 26 mm diameter.6. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA.7. 28 mm diameter.8. Additional dimensions and membrane types are available. Please contact your local Phenomenex technical consultant or distributor for availability or assistance.9. Larger quantity purchases at significant savings are available.
Application / Sample I Recommended Filter I First Alternative I Second Alternative
HPLC and GC Sample Prep RC PTFE PES Aggressive or Pure Organic Solvents PTFE RC NY Protein Analysis/ Biological Samples PES RC GF/CA High Particulate Loads RC NY PTFE Environmental Methods RC PTFE NY Food and Beverage RC PTFE NY Clinical / Toxicology RC PES NY Dissolution Testing RC PTFE NY Ion Chromatography RC PES PTFE Trace Metals (ICP-MS, AAS) RC PES NY Capillary Electrophoresis (CE) RC PES NY Tissue Cultures, Media, Buffers GF/CA PES RC
Syringe Filter Applications and Recommended Membranes
ORDERING INFORMATION
Phenex Syringe Filters 4 mm Diameter 15 mm Diameter 25 mm Diameter for ≤ 2 mL sample volumes for 2 - 10 mL sample volumes for 10 - 100 mL sample volumes Membrane Type/Size Part No. Unit Price Part No. Unit Price Part No. Unit Price 0.20 µm Phenex-RC AF0-3203-12 100/Pk AF0-2203-12 100/Pk AF0-8203-12 5 100/Pk (Regenerated Cellulose) AF0-3203-52 500/Pk AF0-2203-52 500/Pk AF0-8203-52 5 500/Pk Phenex-PES 3 — — — — — — AF0-8208-12 7 100/Pk (Polyethersulfone) — — — — — — AF0-8208-52 7 500/Pk Phenex-PTFE 6 AF0-3202-12 100/Pk AF0-2202-12 100/Pk AF0-1202-12 100/Pk (Polytetrafluoroethylene) AF0-3202-52 500/Pk AF0-2202-52 500/Pk AF0-1202-52 500/Pk Phenex-NY AF3-3207-12 100/Pk AF2-5207-12 1 100/Pk AF0-1207-12 100/Pk (Nylon) AF3-3207-52 500/Pk AF2-5207-52 1 500/Pk AF0-1207-52 500/Pk Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8A09-12 7 100/Pk (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8A09-52 7 500/Pk Outlet connection is luer lock. 0.45 µm Phenex-RC AF0-3103-12 100/Pk AF0-2103-12 100/Pk AF0-8103-12 5 100/Pk (Regenerated Cellulose) AF0-3103-52 500/Pk AF0-2103-52 500/Pk AF0-8103-52 5 500/Pk Phenex-PES 3 — — — AF2-5108-12 1 100/Pk AF0-8108-12 7 100/Pk (Polyethersulfone) — — — — — — AF0-8108-52 7 500/Pk Phenex-PTFE 6 AF0-3102-12 100/Pk AF0-2102-12 100/Pk AF0-1102-12 100/Pk (Polytetrafluoroethylene) AF0-3102-52 500/Pk AF0-2102-52 500/Pk AF0-1102-52 500/Pk Phenex-NY AF3-3107-12 100/Pk AF2-5107-12 1 100/Pk AF0-1107-12 100/Pk (Nylon) AF3-3107-52 500/Pk AF2-5107-52 1 500/Pk AF0-1107-52 500/Pk Phenex-GF/CA 2,3,4 An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a CA membrane. AF0-8B09-12 7 100/Pk (Glass Fiber/Cellulose Acetate) Excellent for filtration of tissue culture media, general biological sample filtration and clarification. AF0-8B09-52 7 500/Pk Outlet connection is luer lock. 1.20 µm Phenex-GF 2,3 Prefiltration of heavily contaminated or highly viscous samples. When used in-line preceding AF0-8515-12 7 100/Pk (Glass Fiber) a membrane filter, clogging of the membrane filter is prevented and sample clean up is optimized. AF0-8515-52 7 500/Pk Outlet connection is luer lock.
Phenomenex | Web: www.phenomenex.com
HP
LC S
ecurityGuard
™
12
SecurityGuard™
• Extends your HPLC column lifetime • Won’t alter your chromatography • Compatible with virtually any HPLC column • Easy to use
SecurityGuard can protect valuable HPLC columns from the damaging effects of chemical contaminants and microparticulates, without altering chromatographic results. This protection can lead to an extension of the column life. Simply put, every chromatographer must have one.
Does Not Alter Your Chromatography
WITH SecurityGuard WITHOUT SecurityGuard
Compare, Virtually No Change!vs.0
0
100
200
300
400
500
mAU
5 20 25 30 min10 15
0
0
100
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300
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500
mAU
5 20 25 30 min10 15
UnIVERSAL GUARD CARtRIDGE SYStEM
0
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100
200
300
400
500
mAU
5 20 25 30 min10 15
0
0
100
200
300
400
500
mAU
5 20 25 30 min10 15
Phenomenex Luna®
Waters Symmetry®
Thermo Fisher HYPERSIL®
Alltech Alltima™
It’s a Universal Fit*
SecurityGuard will fit virtually ANY manufacturer’s HPLC column, making it truly a universal guard system.
*patented
Universal Fit* Floating outlet fits ANY manufacturer’s female/inverted endfitting. Feature applies to analytical-sized guard systemonly, and does not apply to semi-prep or PREPguard cartridges.
Contaminants Trapped
Compounds of InterestCompounds of Interest
ContaminantsContaminants
HP
LC C
olumn
From Injector
Guard
Cartrid
ge System
Cutaway view showingCartridge – can be easily inspected for contaminants
Universal fingertight connection to HPLC column - no wrenches required
One Holder for All
How it Works
Phenomenex | Web: www.phenomenex.com
SP
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SecurityGuard™
UnIVERSAL GUARD CARtRIDGE SYStEM
2.0-3.0
4.0 x 2.0
3.2-8.0
4.0 x 3.0
9.0-16.0
10.0 x 10.0 15.0 x 21.2 15.0 x 30.0
18.0-29.0 30.0-49.0
ANALYTICAL SEMI-PREP PREP
If your HPLC column ID (mm) is:
Use Cartridges (mm):
Material Description pH Stability
Cartridges for General Purpose/Pharmaceutical /10pk /10pk /3pk /ea /ea
C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4286 AJ0-4287 AJ0-7221 AJO-7839 AJO-8301
C12 (Dodecyl) 1.5 - 10 AJ0-6073 AJ0-6074 AJ0-7275 AJO-7842 AJO-8304
C8 (MOS, Octyl) 1.5 - 10 AJ0-4289 AJ0-4290 AJ0-7222 AJO-7840 AJO-8302
C5 (Pentyl) 1.5 - 10 AJ0-4292 AJ0-4293 AJ0-7372 — —
C1 (TMS) 2 - 9 AJ0-4298 AJ0-4299 AJ0-7373 — —
Silica — — AJ0-4347 AJ0-4348 AJ0-7223 AJO-7229 AJO-8312
HILIC HILIC 1.5 - 8 AJ0-8328 AJ0-8329 — — —
NH2 (Amino, Aminopropyl) 1.5 - 11 AJ0-4301 AJ0-4302 AJ0-7364 AJ0-8162 AJO-8309
CN (Cyano, Cyanopropyl) 2 - 7.5 AJ0-4304 AJ0-4305 AJ0-7313 AJ0-8220 AJO-8311
Phenyl (Phenylhexyl) 1.5 - 10 AJ0-4350 AJ0-4351 AJ0-7314 AJO-7841 AJO-8303
PFP(2) (pentafluorophenylpropyl) 1.5 - 8 AJ0-8326 AJ0-8327 AJ0-8376 AJ0-8377 AJ0-8378
SCX (SA, Strong Cation Exchanger) 2.5 - 7.5 AJ0-4307 AJ0-4308 AJ0-7369 — —
SAX (SB, Strong Anion Exchanger) 2.5 - 7.5 AJ0-4310 AJ0-4311 AJ0-7370 — —
RP-1 (Reversed Phase - Polymer) 0 - 14 AJ0-5808 AJ0-5809 AJ0-7368 AJO-8358 —
Polar-RP (Ether-linked Phenyl) 1.5 - 7 AJ0-6075 AJ0-6076 AJ0-7276 AJO-7845 AJO-8307
Fusion-RP (C18 Polar Embedded) 1.5 - 10 AJ0-7556 AJ0-7557 AJ0-7558 AJO-7844 AJO-8306
AQ C18 (Polar Endcapped C18) 1.5 - 7.5 AJ0-7510 AJ0-7511 AJ0-7512 AJO-7843 AJO-8305
Gemini®-NX (C18 TWIN™ Technology) 1 - 12 AJ0-8367 AJ0-8368 AJ0-8369 AJ0-8370 AJ0-8371
Gemini® C18 (C18 TWIN™ Technology) 1 - 12 AJ0-7596 AJ0-7597 AJ0-7598 AJO-7846 AJO-8308
Gemini® C6-Phenyl (C6-Phenyl TWIN Technology) 1 - 12 AJ0-7914 AJ0-7915 — — —
Oligo-RP (C18 TWIN Technology) 1 - 12 AJ0-8134 AJ0-8135 AJ0-8136 AJ0-8210 AJ0-8310
Oligo-WAX (WA, Weak Anion Exchanger) 1.5 - 11 — AJ0-8324 AJ0-8325 — —
Cartridges for Protein and Polypeptide Reversed Phase /10pk /10pk /3pk /ea /ea
For use with silica columns for separation of proteins & peptides, such as Jupiter® (Phenomenex); Vydac® 218TP, 214TP (Alltech Associates, Inc.); SynChropak® 300 C18, C4 (Eprogen, Inc.); Nucleosil® 300 Å C18, C4; HyPErSIL® 300 Å, and other widepore or 300 Å brands.
Widepore C18 (ODS, Octadecyl) 1.5 - 10 AJ0-4320 AJ0-4321 AJ0-7224 AJO-7230 AJO-8313
Widepore C5 (Pentyl) 1.5 - 10 AJ0-4326 AJ0-4327 AJ0-7371 — —
Widepore C4 (Butyl) 1.5 - 10 AJ0-4329 AJ0-4330 AJ0-7225 AJO-7231 AJO-8314
Cartridges for Silica GFC — /10pk /3pk — —(Aqueous SEC) For use with silica GFC columns, such as BioSep™ (Phenomenex); ZorBAx® GF-Series; Bio-Sil® (Bio-rad).
GFC-2000 – 2 - 7.5 — AJ0-4487 AJ0-7365 — —
GFC-3000 – 2 - 7.5 — AJ0-4488 AJ0-7366 — —
GFC-4000 – 2 - 7.5 — AJ0-4489 AJ0-7367 — —
Cartridges for Chiral /10pk /10pk /3pk /ea /ea
For use with chiral columns, such as Lux™ (Cellulose-1 & Cellulose-2 (Phenomenex); CHIrALCEL® oD-H® (DAICEL Chemical industries LTD).
Lux™ Cellulose-1
(Cellulose tris (3, 5-dimethylphenyl-carbamate)
2 - 8 AJ0-8402 AJ0-8403 AJ0-8404 AJ0-8405 AJ0-8406
Lux™ Cellulose-2
(Cellulose tris (3-chloro-4-methyl-phenylcarbamate)
2 - 8 AJ0-8398 AJ0-8366 AJ0-8399 AJ0-8400 AJ0-8401
Cartridges for Carbohydrate/Organic Acid /10pk
For organic acid and carbohydrate analysis, such as rezex™ (Phenomenex); Aminex® (Bio-rad); Interaction; Sugar-Pak™ (Waters).
Carbo-H+ – 1.0 - 7.5 — AJ0-4490 — — —
Carbo-Ag+* – Neutral — AJ0-4491 — — —
Carbo-Pb+2 – Neutral — AJ0-4492 — — —
Carbo-Ca+2 – Neutral — AJ0-4493 — — —
* For use with saccharide and oligosaccharide columns in Ag+ form.
Guard Cartridge Holders (one-time purchase only) /kit /holder /kit /kit
KJ0-4282 AJ0-7220 AJO-8223 AJO-8277
ORDERING INFORMATION
Phenomenex | Web: www.phenomenex.com
Accessories
BioS
eparations
PR
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GC
SP
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Gem
ini ®
14
Gemini®-nX C18 • Control sensitivity and peak purity • Robust method transfers • Decrease purification time
HIGH PERFORMAnCE pH StABLE HPLC COLUMnS
APP
ID 1
6601
pH 7.0 pH 10.5
APP
ID 1
6600 Column: Gemini-NX 5 µm C18
Dimensions: 150 x 4.6 mmPart No.: 00F-4454-E0
Mobile Phase: A: 20 mM Potassium Phosphate pH 7.0B: Acetonitrile
Gradient: A/B (95:5) to (5:95) in 10 min, Hold for 2 min
Flow Rate: 1.5 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: 1.
2.3.
Amitriptyline Naproxen Toluene
Column: Gemini-NX 5 µm C18Dimensions: 150 x 4.6 mm
Part No.: 00F-4454-E0Mobile Phase: A: 10 mM Ammonium
Bicarbonate pH 10.5B: Acetonitrile
Gradient: A/B (95:5) to (5:95) in 10 min, Hold for 2 min
Flow Rate: 1.5 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: 1.
2.3.
Amitriptyline NaproxenToluene
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3
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2
3
1
pH 2.7
APP
ID 1
6593 Column: Gemini-NX 5 µm C18
Dimensions: 150 x 4.6 mmPart No.: 00F-4454-E0
Mobile Phase: A: 0.1 % Formic Acid in WaterB: 0.1 % Formic Acid in Acetonitrile
Gradient: A/B (95:5) to (5:95) in 10 min, Hold for 2 min
Flow Rate: 1.5 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: 1.
2.3.
Amitriptyline NaproxenToluene
pH 4.8
APP
ID 1
6598 Column: Gemini-NX 5 µm C18
Dimensions: 150 x 4.6 mmPart No.: 00F-4454-E0
Mobile Phase: A: 10 mM Ammonium Acetate pH 4.8B: Acetonitrile
Gradient: A/B (95:5) to (5:95) in 10 min, Hold for 2 min
Flow Rate: 1.5 mL/minTemperature: Ambient
Detection: UV @ 254 nmSample: 1.
2.3.
Amitriptyline NaproxenToluene
0 2 4 6
1
2
3
8 10 12 min
0
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800
mAU
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1 2
3
O
SiCH2
CH2
O
O
O
Si S
O
O
Si
O O
O
O
Si
O
O Si Si
O
O
Si
O
O
O
Si Si
O
Si SiOOCH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
C
C
O
SiCH2
CH2
O
O
O
Si Si
O
O
Si
O O
O
O
Si
O
O Si Si
O
O
Si
O
O
O
Si Si
O
Si SiOOCH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
CH2
C
Second-Generation TWIN-NX™ TechnologyGemini-NX uses an improved patent pending organo-silica grafting process which incorporates highly stabilizing ethane cross-linking. These organic groups are evenly incorporated into the grafted layers on the silica surface while maintaining a pure silica core. This not only provides resistance to high pH attack, but also maintains the high efficiency and mechanical strength of a silica particle.
The uniform TWIN-NX grafting technology presents an ordered surface silane distribution. This provides homogeneously active bonding sites ideal for low pH resistant, multi-point ligand attachments.
Create Compound Optimized Methods Using pH
new
Gemini-NX is Patent Pending.
Phenomenex | Web: www.phenomenex.com
SP
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Gem
ini®
GC
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HIGH PERFORMAnCE pH StABLE HPLC COLUMnS
For more information about SecurityGuard see pp. 12-13
Gemini®-nX C18 (cont’d)
Excellent Performance in Volatile Buffers!
APP
ID 1
6618 Gemini®-NX 5 µm C18
0 2 4 6 8 10 min
1
2
3
4
5
6
7
8
9
10Excellent Performance in
Non-Volatile Buffers!
APP
ID 1
6616 Gemini®-NX 5 µm C18
0 2 4 6 8 10 12 min
1
2
3
4
5
6
78
9
10
Mixtures of Acids, Neutrals, and Bases in Formic Acid pH 2.7
Column: Gemini-NX 5 µm C18Dimensions: 150 x 4.6 mm
Part No.: 00F-4454-E0Mobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in AcetonitrileGradient: A/B (95:5) to (20:80) in 8 min,
Hold for 2 minFlow Rate: 1.5 mL/min
Temperature: AmbientDetection: UV @ 254 nm
Sample: 1.2.3.4.5.6.7.8.9.
10.
PyridineQuinidineSulfathiazoleTriprolidineBenzyl alcoholNortriptylinePhenol3-Methyl-4-nitrobenzoic acidMethylsalicylaldehydeHexanophenone
Mixtures of Acids, Neutrals, and Bases in Potassium Phosphate pH 2.5
Column: Gemini-NX 5 µm C18Dimensions: 150 x 4.6 mm
Part No.: 00F-4454-E0Mobile Phase: A: 20 mM Potassium Phosphate pH 2.5
B: AcetonitrileGradient: A/B (95:5) to (20:80) in 8 min,
Hold for 2 minFlow Rate: 1.5 mL/min
Temperature: AmbientDetection: UV @ 254 nm
Sample: 1.2.3.4.5.6.7.8.9.
10.
PyridineQuinidineSulfathiazoleTriprolidineBenzyl alcoholPhenol Nortriptyline3-Methyl-4-nitrobenzoic acidMethylsalicylaldehydeHexanophenone
Robust Method Transfers
10 μm Axia™ Packed Preparative Columns (mm) continued SecurityGuard™Cartridges (mm)Phases 250 x 30 50 x 50 100 x 50 150 x 50 250 x 50 15 x 21.2** 15 x 30.0
/ea /ea
C18 00G-4455-U0-AX 00B-4455-V0-AX 00D-4455-V0-AX 00F-4455-V0-AX 00G-4455-V0-AX AJ0-8370 AJ0-8371
for ID: 18-29 mm 30-49 mm
5 μm Axia™ Packed Preparative Columns (mm) continued SecurityGuard™ Cartridges (mm)Phases 75 x 30 100 x 30 150 x 30 250 x 30 50 x 50 15 x 21.2** 15 x 30.0
/ea /ea
C18 00C-4454-U0-AX 00D-4454-U0-AX 00F-4454-U0-AX 00G-4454-U0-AX 00B-4454-V0-AX AJ0-8370 AJ0-8371
for ID: 18.0-29 mm 30-49 mm
10 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™Cartridges (mm)Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 100 x 30 150 x 30 15 x 21.2** 15 x 30.0
/ea /ea
C18 00B-4455-P0-AX 00D-4455-P0-AX 00F-4455-P0-AX 00G-4455-P0-AX 00D-4455-U0-AX 00F-4455-U0-AX AJ0-8370 AJ0-8371
for ID: 18-29 mm 30-49 mm
3 μm Minibore Columns (mm) SecurityGuard™ Cartridges (mm)Phases 20 x 2.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0*
/10pkC18 00M-4453-B0 00A-4453-B0 00B-4453-B0 00D-4453-B0 00F-4453-B0 AJ0-8367
for ID: 2.0-3.0 mm
5 μm Minibore Columns (mm) SecurityGuard™ Cartridges (mm)Phases 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0*
/10pk
C18 00A-4454-B0 00B-4454-B0 00D-4454-B0 00F-4454-B0 AJ0-8367
for ID: 2.0-3.0 mm
3 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)Phases 30 x 3.0 50 x 3.0 100 x 3.0 150 x 3.0 50 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*
/10pk /10pk
C18 00A-4453-Y0 00B-4453-Y0 00D-4453-Y0 00F-4453-Y0 00B-4453-E0 00D-4453-E0 00F-4453-E0 00G-4453-E0 AJ0-8367 AJ0-8368
for ID: 2.0-3.0 mm 3.2-8.0 mm
5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phases 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 50 x 30 15 x 21.2** 15 x 30.0
/ea /ea
C18 00B-4454-P0-AX 00D-4454-P0-AX 00F-4454-P0-AX 00G-4454-P0-AX 00B-4454-U0-AX AJ0-8370 AJ0-8371
for ID: 18.0-29 mm 30-49 mm
5 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)Phases 50 x 3.0 100 x 3.0 150 x 3.0 250 x 3.0 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*
/10pk /10pk
C18 00B-4454-Y0 00D-4454-Y0 00F-4454-Y0 00G-4454-Y0 00B-4454-E0 00C-4454-E0 00D-4454-E0 00F-4454-E0 00G-4454-E0 AJ0-8367 AJ0-8368
for ID: 2.0-3.0 mm 3.2-8.0 mm
*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277
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TM
Onyx™ Analytical & Semi-Prep Monolithic Columns • 2 mm ID column for improved sensitivity and less solvent usage • Reduce run times by more than 50 % • Eliminate time consuming sample preparation by using “dilute-and-shoot” methods • Extremely low backpressures preserves column and system lifetime
Traditional Silica “Particle-Based” Column
• Individual silica particles
• High flow resistance: Limits ability to shorten run times
• High backpressure: Reduces life of pumps, seals, and column
• Reduced throughput: Long run times
• Bed splitting possible: Shortens column life and lessens reproducibility
“Monolithic” Column
• Monolithic porous silica rod
• High flow rates: Due to high porosity
• Low backpressure: Less stress on system and column
• Increased throughput: Significantly shorter run times
• No inlet bed settling: Increased reliability, reproducibility, and lifetime
ORDERING INFORMATION
Monolithic Silica HPLC ColumnsAnalytical ColumnsPart No. Description Size (mm) Price
CH0-8373 Onyx Monolithic C18 50 x 2.0
CH0-8158 Onyx Monolithic C18 100 x 3.0
CH0-7643 Onyx Monolithic C18 100 x 4.6
CH0-7644 Onyx Monolithic C18 50 x 4.6
CH0-7645 Onyx Monolithic C18 25 x 4.6
CH0-7647 Onyx Monolithic C8 100 x 4.6
CH0-7648 Onyx Monolithic Si 100 x 4.6
SemiPrep ColumnsPart No. Description Size (mm) Price
CH0-7878 Onyx SemiPrep Monolithic C18 100 x 10.0
FASt HIGH-tHROUGHPUt SEPARAtIOnS
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App ID: 15585
mAU
Onyx™ Capillary Columns • Ideal for peptide mapping • Load samples at µL/min flow rates to significantly decrease cycle time • Trap loading unnecessary due to minimal carryover • Long lifetime when working with biological samples
TM
Tryptic Digest of b-Amylase
b-Amylase is digested by Trypsin to generate a complex peptide map. No desalting or trap column is used, demonstrating Onyx’s resistance to matrix contaminants.
• The monolithic phase of Onyx delivers high peak efficiency and good peak shape even in a capillary format.
• Onyx monolithic silica allows for wide flow rate flexibility resulting in loading at 10 µL/min flow rates. A 20 µL sample can be directly loaded in less than 2 minutes without the use of a trap column.
ORDERING INFORMATION
Monolithic Silica HPLC Columns
Capillary Columns
Part No. Description Size (mm) Price
CH0-8388 Onyx Monolithic C18 150 x 0.05
CH0-7646 Onyx Monolithic C18 150 x 0.1
CH0-8389 Onyx Monolithic HD-C18 150 x 0.1
CH0-8390 Onyx Monolithic C18 150 x 0.2
CH0-8391 Onyx Monolithic HD-C18 150 x 0.2
CH0-8392 Onyx Monolithic C18 Trapping Column 150 x 0.2
CH0-8393 Onyx Monolithic C8 150 x 0.1
Conditions: Column: Onyx Monolithic C18 Dimensions: 150 x 0.1 mm Part No.: CH0-7646 Mobile Phase: A: 0.1 % TFA in Water B: 0.085 % TFA in Water / 95 % Acetonitrile Gradient: 5-65 % B in 60 minutes Flow Rate: 3.0 µL/min Temperature: Ambient Detection: UV @ 210 nm Sample: b-Amylase Tryptic Digest
FASt HIGH-tHROUGHPUt SEPARAtIOnS
Ap
p ID
: 155
85
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BioS
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Accessories
18
™
Synergi™ 4 µm Method Development Columns • Simplified column selection • High efficiency at low backpressures • Hydrophobic/philic and aromatic retention
Four Unique Phases to Advance Your LC Selectivity
ORDERING INFORMATION 4 µm Microbore Columns (mm)Phase 50 x 1.0 150 x 1.0 250 x 1.0
Max-RP 00B-4337-A0 00F-4337-A0 —
Hydro-RP 00B-4375-A0 00F-4375-A0 00G-4375-A0
Polar-RP 00B-4336-A0 00F-4336-A0 —
Fusion-RP 00B-4424-A0 00F-4424-A0 —SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282
4 µm Minibore and Narrow Bore Columns (mm) SecurityGuard™ CartridgesPhase 50 x 2.0 150 x 2.0 50 x 3.0 150 x 3.0 250 x 3.0 4 x 2.0 mm
/10pk
Max-RP 00B-4337-B0 00F-4337-B0 00B-4337-Y0 00F-4337-Y0 00G-4337-Y0 AJ0-6073
Hydro-RP 00B-4375-B0 00F-4375-B0 00B-4375-Y0 00F-4375-Y0 00G-4375-Y0 AJ0-7510
Polar-RP 00B-4336-B0 00F-4336-B0 00B-4336-Y0 00F-4336-Y0 00G-4336-Y0 AJ0-6075
Fusion-RP 00B-4424-B0 00F-4424-B0 00B-4424-Y0 00F-4424-Y0 00G-4424-Y0 AJ0-7556
for ID: 2.0-3.0 mm
4 µm Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 4.6 50 x 4.6 75 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0 mm
/10pk
Max-RP 00A-4337-E0 00B-4337-E0 00C-4337-E0 00F-4337-E0 00G-4337-E0 AJ0-6074
Hydro-RP 00A-4375-E0 00B-4375-E0 00C-4375-E0 00F-4375-E0 00G-4375-E0 AJ0-7511
Polar-RP 00A-4336-E0 00B-4336-E0 00C-4336-E0 00F-4336-E0 00G-4336-E0 AJ0-6076
Fusion-RP 00A-4424-E0 00B-4424-E0 00C-4424-E0 00F-4424-E0 00G-4424-E0 AJ0-7557
for ID: 3.2-8.0 mm
4 µm Axia™ Packed Columns (mm) SecurityGuard™ CartridgesPhase 50 x 21.2 100 x 21.2 150 x 21.2 250 x 21.2 100 x 30 15 x 21.2** mm 15 x 30.0♦ mm
Max-RP 00B-4337-P0-AX 00D-4337-P0-AX 00F-4337-P0-AX 00G-4337-P0-AX 00D-4337-U0-AX AJ0-7842 AJ0-8304
Hydro-RP 00B-4375-P0-AX 00D-4375-P0-AX 00F-4375-P0-AX 00G-4375-P0-AX 00D-4375-U0-AX AJ0-7843 AJ0-8305
Polar-RP 00B-4336-P0-AX 00D-4336-P0-AX 00F-4336-P0-AX 00G-4336-P0-AX 00D-4336-U0-AX AJ0-7845 AJ0-8307
Fusion-RP 00B-4424-P0-AX 00D-4424-P0-AX 00F-4424-P0-AX 00G-4424-P0-AX 00D-4424-U0-AX AJ0-7844 AJ0-8306
for ID: 18-29 mm 30-49 mm
SIMPLIFIED HPLC MEtHOD DEVELOPMEnt
Advancing LC Selectivity
Hydrogen Bonding
Silanol ActivityMultiplied by 2
MethyleneSelectivityMultiplied by 3
Polar SelectivityHydrophobicityDivided by 5
0.800
0.700
0.600
0.500
0.400
0.300
0.200
0.100
0.000
Columns: Synergi 4 µm Fusion-RP Synergi 4 µm Max-RP Synergi 4 µm Hydro-RP Synergi 4 µm Polar-RPDimensions: 150 x 4.6 mm Mobile Phase: 1. Water / Methanol (20:80) for k Amylbenzene
2. Water / Methanol (20:80) for a (CH2) 3. Water / Methanol (70:30) for a C/P 4. Methanol / 20 mM Potassium phosphate pH 2.7 for a B/P, pH 2.75. 5. Methanol /20 mM Potassium phosphate pH 7.6 for a B/P, pH 7.6
Flow Rate: 1.0 mL/minDetection: UV @ 254 nmInjection: 5 mL Temperature: 30 °CSample: 1. Amylbenzene
2. Amylbenzene and Butylbenzene 3. Caffeine and phenol (C/P) 4. Benzylamine and phenol (B/P) @ pH 2.75 5. Benzylamine and phenol (B/P) @ pH 7.6
Synergi Fusion-RPSynergi Max-RPSynergi Hydro-RPSynergi Polar-RP
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277
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*4 μm Synergi materials available upon request. ** Requires MercuryMS Cartridge Holder
0.5 1.0 1.5 2.0 min0
50
100
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250
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400
Intensity,cps
0.2 0.4 0.6 0.8 1.0 1.2 1.4 min0
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Intensity,cps
0.5 1.0 1.5 2.0 min0
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Intensity,cps
0.2 0.4 0.6 0.8 1.0 1.2 1.4 min0
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Intensity,cps
Synergi™ 2.5 µm MercuryMS™
• Perform ultra-fast separations on your HPLC • High efficiency in small format • Achieve 2x increase in sensitivity
™
Synergi Polar-RP 4 μm 50 x 2.0 mm
1 mL/min, 100 bar 2.1 min run time
Synergi Polar-RP 2.5 μm 20 x 2.0 mm
1 mL/min, 220 bar 1.3 min run time
Data courtesy of GSK 2005
SIMPLIFIED HPLC MEtHOD DEVELOPMEnt
Same LC/MS
Advancing LC Selectivity
ORDERING INFORMATION
• 100 % Sensitivity gain • 30 % Reduction in run time
Cartridges (mm)** Columns (mm)Phase 10 x 2.0 10 x 4.0 20 x 20 20 x 4.0 20 x 2.0 20 x 4.0
2.5 μm PhaseSynergi Max-RP* 00N-4372-B0-CE 00N-4372-D0-E0 00M-4372-B0-CE 00M-4372-D0-CE 00M-4372-B0 00M-4372-D0
Synergi Hydro-RP* 00N-4387-B0-CE 00N-4387-D0-E0 00M-4387-B0-CE 00M-4387-D0-CE 00M-4387-B0 00M-4387-D0
Synergi Polar-RP* — 00N-4371-D0-CE 00M-4371-B0-CE 00M-4371-D0-CE 00M-4371-B0 00M-4371-D0
Synergi Fusion-RP* 00N-4423-B0-CE 00N-4423-D0-CE 00M-4423-B0-CE 00M-4423-D0-CE 00M-4423-B0 00M-4423-D0
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HP
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ST (H
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HSt: High Speed technology • Ultra-fast results on your current HPLC system • Easy method transfer
HIGH SPEED tECHnOLOGY
High Speed Technology
Synergi 4 µm Polar-RP Synergi 2.5 µm Polar-RP-HST
1
0 1.0 min
0 10 20 min
0 10 20 30 min
23
4
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7 8 9
12 3
4 56
7 8 9
1
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0 10 20 30 min
23
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7 8 9
12 3
4 56
7 8 9
Column: Synergi 2.5 µm Polar-RP-HSTDimensions: 50 x 2.0 mmPart No.: 00B-4371-B0 Mobile Phase: A: Water
B: AcetonitrileGradient: 10 % B hold for 0.5 min, to 95 % B
in 1.5 min, hold for 1 min, then to 10 % B in 0.1 min (or 1 sec.)
Flow Rate: 1.2 mL/minDetection: UV @ 270 nm; 1.9 min
re-equilibrationInjection: 1 µL Temperature: 60 °CSample: 20 mg/mL in Acetonitrile
1. Acetone 2. 2-Butanone 3. 2-Pentanone 4. 2-Hexanone 5. 2-Heptanone 6. 2-Octanone 7. 2-Decanone 8. 2-Tridecanone 9. 2-Hexadecanone
10 x Increase in speed
!
Column: Synergi 4 µm Polar-RPDimensions: 250 x 4.6 mmPart No.: 00G-4336-E0 Mobile Phase: A: Water
B: AcetonitrileGradient: 10 % B hold for 1 min, to 95 % B
in 25 min, hold for 10 min, then to 10 % B in 0.1 min
Flow Rate: 1.5 mL/minDetection: UV @ 270 nm; 9.9 min
re-equilibrationInjection: 5 µLTemperature: 60 °CSample: 20 mg/mL in Acetonitrile
1. Acetone 2. 2-Butanone 3. 2-Pentanone 4. 2-Hexanone 5. 2-Heptanone 6. 2-Octanone 7. 2-Decanone 8. 2- Tridecanone 9. 2-Hexadecanone
Synergi 4 µm Polar-RP250 x 4.6 mm
App ID 16263
App ID 16262
Synergi 2.5 µm Polar-RP-HST50 x 2.0 mm
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ORDERInG InFORMAtIOn
High Speed technology (HSt) Columns (mm)
30 x 2.0 50 x 2.0 100 x 2.0 50 x 3.0 100 x 3.0 50 x 4.6
Phases
Luna 2.5 µm C18(2)-HST 00A-4446-B0 00B-4446-B0 00D-4446-B0 00B-4446-Y0 00D-4446-Y0 ––
Alternative Selectivities
Synergi 2.5 µm Max-RP 00A-4372-B0 00B-4372-B0 00D-4372-B0 00B-4372-Y0 00D-4372-Y0 00B-4372-E0
Synergi 2.5 µm Polar-RP 00A-4371-B0 00B-4371-B0 00D-4371-B0 00B-4371-Y0 00D-4371-Y0 00B-4371-E0
Synergi 2.5 µm Hydro-RP 00A-4387-B0 00B-4387-B0 00D-4387-B0 00B-4387-Y0 00D-4387-Y0 00B-4387-E0
Synergi 2.5 µm Fusion-RP 00A-4423-B0 00B-4423-B0 00D-4423-B0 00B-4423-Y0 00D-4423-Y0 00B-4423-E0
HIGH SPEED tECHnOLOGY
High Speed Technology
HSt Columns• High performance efficiencies comparable to sub-2 µm particle size columns at roughly half the backpressure
0
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Flow Rate: 0.55 mL/min
0
1
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5
0.4 0.8 min
Luna 2.5 µm C18(2)-HST
254 bar
Competitor A 1.7 µm C18
Luna 2.5 µm C18(2)-HST
Competitor A 1.7 µm C18Flow Rate: 0.55 mL/min
0
1
23 4
5
0.4 0.8 min
400 bar
Linear Velocity (mm/s)
Flow Rate: 1.00 mL/min
400 bar
0.20
1
23 4
5
0.4 0.6 min
Luna 2.5 µm C18(2)-HST
Competitor A 1.7 µm C18Flow Rate: 1.00 mL/min
0
2
1
3 45
0.2 0.4 0.6 min
850 bar
Conditions: (same for both columns) Columns: Luna 2.5 µm C18(2)-HST Competitor A 1.7 µm C18 Dimension: 50 x 2.0 mm (Luna) 50 x 2.1 mm (Competitor A) Mobile Phase: Acetonitrile / Water (65:35) Flow Rate: As noted Detection: UV @ 254 nm Temperature: 30 ºC Sample: 1. Uracil 2. Acetophenone 3. Benzene 4. Toluene 5. Naphthalene
To learn more about Synergi phases, see page 18 of this guide or visit www.phenomenex.com
APP
ID 1
6271
APP
ID 1
6272
APP
ID 1
6269
APP
ID 1
6270
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OUR tOP SELLInG HPLC COLUMn
Luna®
• Easy method scalability with 2.5, 3, 5, 10, 10-PREP, and 15 µm medias • Extensive batch traceability and reproducibility data supplied with every column • Exceptionally rugged USP phases
ORDERING INFORMATION
3 µm Microbore and Minibore Columns (mm) SecurityGuard™ CartridgesPhase 30 x 1.0 50 x 1.0 150 x 1.0 30 x 2.0 50 x 2.0 100 x 2.0 150 x 2.0 4 x 2.0 mm SecurityGuard
Cartridges require universal holder
Part No.: KJO-4282
/10pk
Silica(2) — — — 00A-4162-B0 00B-4162-B0 00D-4162-B0 00F-4162-B0 AJ0-4347
C8(2) 00A-4248-A0 00B-4248-A0 00F-4248-A0 00A-4248-B0 00B-4248-B0 00D-4248-B0 00F-4248-B0 AJ0-4289
C18(2) 00A-4251-A0 00B-4251-A0 00F-4251-A0 00A-4251-B0 00B-4251-B0 00D-4251-B0 00F-4251-B0 AJ0-4286
CN — — — 00A-4254-B0 00B-4254-B0 00D-4254-B0 00F-4254-B0 AJ0-4304
Phenyl-Hexyl 00A-4256-A0 00B-4256-A0 — 00A-4256-B0 00B-4256-B0 00D-4256-B0 00F-4256-B0 AJ0-4350
NH2 — — 00F-4377-A0 00A-4377-B0 00B-4377-B0 00D-4377-B0 00F-4377-B0 AJ0-4301
for ID: 2.0-3.0 mm
3 µm Narrow Bore and Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 3.0 50 x 3.0 150 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 100 x 4.6 150 x 4.6 4 x 2.0 mm 4 x 3.0 mm
/10pk /10pk
Silica(2) — — 00F-4162-Y0 — 00B-4162-E0 — 00D-4162-E0 00F-4162-E0 AJ0-4347 AJ0-4348
C8(2) 00A-4248-Y0 00B-4248-Y0 00F-4248-Y0 00A-4248-E0 00B-4248-E0 00C-4248-E0 00D-4248-E0 00F-4248-E0 AJ0-4289 AJ0-4290
C18(2) 00A-4251-Y0 00B-4251-Y0 00F-4251-Y0 00A-4251-E0 00B-4251-E0 00C-4251-E0 00D-4251-E0 00F-4251-E0 AJ0-4286 AJ0-4287
CN — 00B-4254-Y0 00F-4254-Y0 00A-4254-E0 00B-4254-E0 00C-4254-E0 00D-4254-E0 00F-4254-E0 AJ0-4304 AJ0-4305
Phenyl-Hexyl — 00B-4256-Y0 00F-4256-Y0 00A-4256-E0 00B-4256-E0 00C-4256-E0 00D-4256-E0 00F-4256-E0 AJ0-4350 AJ0-4351
NH2 — 00B-4377-Y0 00F-4377-Y0 00A-4377-E0 00B-4377-E0 — 00D-4377-E0 00F-4377-E0 AJ0-4301 AJ0-4302
for ID: 2.0-3.0 mm for ID: 3.2-8.0 mm
5 µm Microbore and Minibore Columns (mm) SecurityGuard™ CartridgesPhase 50 x 1.0 150 x 1.0 250 x 1.0 30 x 2.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0 mm SecurityGuard
Cartridges require universal holder
Part No.: KJO-4282
/10pk
Silica(2) — — — — 00B-4274-B0 00F-4274-B0 00G-4274-B0 AJ0-4347
C5 — — — 00A-4043-B0 00B-4043-B0 00F-4043-B0 — AJ0-4292
C8 — — — 00A-4040-B0 00B-4040-B0 00F-4040-B0 — AJ0-4289
C8(2) 00B-4249-A0 00F-4249-A0 00G-4249-A0 00A-4249-B0 00B-4249-B0 00F-4249-B0 00G-4249-B0 AJ0-4289
C18 — — — 00A-4041-B0 00B-4041-B0 00F-4041-B0 00G-4041-B0 AJ0-4286
C18(2) 00B-4252-A0 00F-4252-A0 00G-4252-A0 00A-4252-B0 00B-4252-B0 00F-4252-B0 00G-4252-B0 AJ0-4286
CN — — — 00A-4255-B0 00B-4255-B0 00F-4255-B0 00G-4255-B0 AJ0-4304
Phenyl-Hexyl 00B-4257-A0 00F-4257-A0 00G-4257-A0 00A-4257-B0 00B-4257-B0 00F-4257-B0 00G-4257-B0 AJ0-4350
NH2 — 00F-4378-A0 — 00A-4378-B0 00B-4378-B0 00F-4378-B0 00G-4378-B0 AJ0-4301
for ID: 2.0-3.0 mm
5 µm Narrow Bore and Analytical Columns (mm) SecurityGuard™ CartridgesPhase 30 x 3.0 50 x 3.0 150 x 3.0 250 x 3.0 30 x 4.6 50 x 4.6 75 x 4.6 4 x 2.0 mm 4 x 3.0 mm SecurityGuard
Cartridges require universal holder.
Part No.: KJO-4282
/10pk /10pk
C8 — — 00F-4040-Y0 00G-4040-Y0 00A-4040-E0 00B-4040-E0 00C-4040-E0 AJ0-4289 AJ0-4290
C8(2) — 00B-4249-Y0 00F-4249-Y0 00G-4249-Y0 00A-4249-E0 00B-4249-E0 00C-4249-E0 AJ0-4289 AJ0-4290
C18 — — 00F-4041-Y0 00G-4041-Y0 00A-4041-E0 00B-4041-E0 00C-4041-E0 AJ0-4286 AJ0-4287
C18(2) 00A-4252-Y0 00B-4252-Y0 00F-4252-Y0 00G-4252-Y0 00A-4252-E0 00B-4252-E0 00C-4252-E0 AJ0-4286 AJ0-4287
CN — 00B-4255-Y0 00F-4255-Y0 00G-4255-Y0 00A-4255-E0 00B-4255-E0 00C-4255-E0 AJ0-4304 AJ0-4305
Phenyl-Hexyl 00A-4257-Y0 00B-4257-Y0 00F-4257-Y0 00G-4257-Y0 00A-4257-E0 00B-4257-E0 00C-4257-E0 AJ0-4350 AJ0-4351
NH2 — 00B-4378-Y0 00F-4378-Y0 00G-4378-Y0 00A-4378-E0 00B-4378-E0 00C-4378-E0 AJ0-4301 AJ0-4302
SCX — — — — — 00B-4398-E0 — AJ0-4307 AJ0-4308
for ID: 2.0-3.0 mm for ID: 3.2-8.0 mm
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Luna®
Method Validation Document
over 25 individual Chemical & Physical
tests!
Luna®
OUR tOP SELLInG HPLC COLUMn
For bulk media pricing and availability, please contact the Phenomenex Prep
Team at [email protected]
15 µm Preparative and Pilot Scale Columns (mm)Column Guards
Phase 250 x 4.6 250 x 21.2 250 x 30 250 x 50 50 x 50
Silica(2) 00G-4271-E0 00G-4271-P0 00G-4271-U0 00G-4271-V0 03B-4271-V0
C8(2) 00G-4272-E0 00G-4272-P0 00G-4272-U0 00G-4272-V0 03B-4272-V0
C18(2) 00G-4273-E0 00G-4273-P0 00G-4273-U0 00G-4273-V0 03B-4273-V0
Phenyl-Hexyl 00G-4286-E0 00G-4286-P0 00G-4286-U0 00G-4286-V0 03B-4286-V0
ORDERING INFORMATION
5 µm Analytical, Semi-Prep and Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phase 100 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 4 x 3.0* 10 x 10‡
/10pk /3pk
Silica(2) 00D-4274-E0 00F-4274-E0 00G-4274-E0 00G-4274-N0 — 00G-4274-P0-AX 00G-4274-U0-AX AJ0-4348 AJ0-7223
C5 00D-4043-E0 00F-4043-E0 00G-4043-E0 — — — — AJ0-4293 AJO-7372
C8 00D-4040-E0 00F-4040-E0 00G-4040-E0 00G-4040-N0 — — — AJ0-4290 AJ0-7222
C8(2) 00D-4249-E0 00F-4249-E0 00G-4249-E0 00G-4249-N0 00G-4249-AK 00G-4249-P0-AX 00G-4249-UO-AX AJ0-4290 AJ0-7222
C18 00D-4041-E0 00F-4041-E0 00G-4041-E0 00G-4041-N0 — — — AJ0-4287 AJ0-7221
C18(2) 00D-4252-E0 00F-4252-E0 00G-4252-E0 00G-4252-N0 00G-4252-AK 00G-4252-P0-AX 00G-4252-U0-AX AJ0-4287 AJ0-7221
CN 00D-4255-E0 00F-4255-E0 00G-4255-E0 00G-4255-N0 — 00G-4255-P0-AX — AJ0-4305 AJ0-7313
Phenyl-Hexyl 00D-4257-E0 00F-4257-E0 00G-4257-E0 00G-4257-N0 — 00G-4257-P0-AX 00G-4257-U0-AX AJ0-4351 AJ0-7314
NH2 00D-4378-E0 00F-4378-E0 00G-4378-E0 00G-4378-N0 — 00G-4378-P0-AX 00G-4378-U0-AX AJ0-4302 AJ0-7364
SCX 00D-4398-E0 00F-4398-E0 00G-4398-E0 00G-4398-N0 — 00G-4398-P0-AX — AJ0-4308 AJ0-7369
for ID: 3.2-8.0 mm for ID: 9-16 mm
10 µm Semi-Prep and Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)Phase 250 x 4.6 250 x 10 250 x 15 250 x 21.2 250 x 30 250 x 50 10 x 10‡ 15 x 21.2** 15 x 30.0♦
/3pk /ea /ea
Silica(2) 00G-4091-E0 00G-4091-N0 — 00G-4091-P0-AX 00G-4091-U0-AX — AJO-7223 AJ0-7229 AJ0-8312
C5 00G-4092-E0 00G-4092-N0 — 00G-4092-P0-AX — — AJO-7372 — —
C8 00G-4093-E0 00G-4093-N0 — 00G-4093-P0-AX — — AJO-7222 AJ0-7840 AJ0-8302
C8(2) 00G-4250-E0 00G-4250-N0 — 00G-4250-P0-AX 00G-4250-U0-AX 00G-4250-V0-AX AJO-7222 AJO-7840 AJ0-8302
C18 00G-4094-E0 00G-4094-N0 — 00G-4094-P0-AX — — AJO-7221 AJ0-7839 AJ0-8301
C18(2) 00G-4253-E0 00G-4253-N0 00G-4253-AK 00G-4253-P0-AX 00G-4253-U0-AX 00G-4253-V0-AX AJO-7221 AJO-7839 AJ0-8301
CN 00G-4300-E0 00G-4300-N0 — 00G-4300-P0-AX — — AJO-7313 AJO-8220 AJ0-8311
Phenyl-Hexyl 00G-4285-E0 00G-4285-N0 — 00G-4285-P0-AX 00G-4285-U0-AX — AJ0-7314 AJO-7841 AJ0-8303
NH2 00G-4379-E0 00G-4379-N0 — 00G-4379-P0-AX — — AJ0-7364 AJO-8162 AJ0-8309
SCX 00G-4401-E0 00G-4401-N0 — 00G-4401-P0-AX — — AJ0-7369 — —
for ID: 9-16 mm 18-29 mm 30-49 mm
For Luna HILIC, see p. 24For Luna PFP(2), see p. 25
*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282‡SemiPrep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277
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SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282
HPLC - SMALL MOLECULE
Luna® HILIC • Retains polar compounds • Elute in high organic mobile phase for optimized mass spectrometry results • Reproducible performance
0.50 1.0 1.5 2.0
1
1
2
2
3
3
2.5 3.0 3.5 4.0 4.5 min0
1000
2000
3000
4000
5000
6000
7000
0.50 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min0
1000
2000
3000
4000
5000
6000
7000
Ap
p ID
163
34
Ap
p ID
163
44
0.50 1.0 1.5 2.0
1
1
2
2
3
3
2.5 3.0 3.5 4.0 4.5 min0
1000
2000
3000
4000
5000
6000
7000
0.50 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 min0
1000
2000
3000
4000
5000
6000
7000
Ap
p ID
163
34
Ap
p ID
163
44
Column: Luna 5 µm HILICDimensions: 100 x 2.0 mmMobile Phase:
A: 90:10 Acetonitrile / 100 mM NH Formate, pH 3.2B: 50:50 Acetonitrile / 20 mM NH Formate, pH 3.2
Gradient: 100:0 A/B for 1 min100:0 A/B to 0:100 A/B in 2 min 0:100 A/B for 1.5 min100:0 A/B for 5 min
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: MS
Sample: 1. Morphine-3-b-Glucuronide 2. Normorphine 3. Morphine
Column: Gemini 5 µm C18Dimensions: 100 x 2.0 mmMobile Phase:
A: Water with 0.1 % Formic acidB: Acetonitrile with 0.1 % Formic acid
Gradient: 97:3 A/B for 1 min97:3 A/B to 70:30 A/B in 2 min97:3 A/B for 2.49 min
Reversed Phase LC
HILIC
HILIC Mode LC
O
C
O
O
N
CH3
OH
OH
HO
HO O
OH
Morphine-3-b-Glucuronide
O
NH
HO
HO
H
Normorphine
O
NCH3
H
HO
HO
Morphine
Reversal of Elution Order. Longer Retention for Glucuronide!
Interested in exploring HILIC chromatography? Request the HILIC Method Screening Approach paper from your authorized Phenomenex distributor.
Polar Retention & Complimentary Selectivity to Reversed Phase
Luna 3 µm HILIC SecurityGuard™ Cartridges (mm)
Part No. Dimensions (mm) /10pk
00B-4449-B0 50 x 2.0 AJ0-8328
00D-4449-B0 100 x 2.0 AJ0-8328
00F-4449-B0 150 x 2.0 AJ0-8328
00B-4449-Y0 50 x 3.0 AJ0-8328
00D-4449-Y0 100 x 3.0 AJ0-8328
00F-4449-Y0 150 x 3.0 AJ0-8328
00D-4449-E0 100 x 4.6 AJ0-8329
00F-4449-E0 150 x 4.6 AJ0-8329
Luna 5 µm HILICPart No. Dimensions (mm) /10pk
00F-4450-Y0 150 x 3.0 AJ0-8328
00D-4450-E0 100 x 4.6 AJ0-8329
00F-4450-E0 150 x 4.6 AJ0-8329
00G-4450-E0 250 x 4.6 AJ0-8329
ORDERING INFORMATION
Flow Rate: 0.4 mL/minTemperature: Ambient
Detection: MS
Sample: 1. Morphine-3-b-Glucuronide 2. Normorphine 3. Morphine
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HPLC - SMALL MOLECULE
Luna® PFP(2) • Multiple retention mechanisms for alternate selectivity • Orthogonal selectivity (to traditional C18 phases) for more success in your method screening • Unique polar interactions for trace impurity identification
Positional Isomers of Halogenated Phenols on Luna 3 µm PFP(2)
Column: Luna 3 µm PFP(2) Dimension: 150 x 4.6 mm Part No.: 00F-4447-E0 Mobile Phase: A: 0.1 % Formic acid in Water B: 0.1 % Formic acid in Acetonitrile Gradient: A/B (60:40) to (50:50) in 10 min Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. 2,3-Dimethylphenol 6. 2,5-Dichlorophenol 2. 2,5-Dimethylphenol 7. 2,6-Dichlorophenol 3. 2,6-Dimethylphenol 8. 3,4-Dichlorophenol 4. 3,4-Dimethylphenol 9. 3,5-Dichlorophenol 5. 3,5-Dimethylphenol 10. 2,4-Dibromophenol
12
34
5
6
7
8
9
10
0 2 4 6 8 10 12 14 min
baseline ! separation
App
ID: 1
6296
16297
0 2 4 6 8 10 12 14 min
12
34
5
67
8
9 & 10
Positional Isomers of Halogenated Phenols on Luna 3 µm C18(2)
Column: Luna 3 µm C18(2) Dimension: 150 x 4.6 mm ID Part No.: 00F-4251-E0 Mobile Phase: A: 0.1% Formic acid in Water B: 0.1% Formic acid in Acetonitrile Gradient: A/B (60:40) to (50:50) in 10 min Flow Rate: 1 mL/min Temperature: 22 °C Detection: UV @ 254 nm Sample: 1. 2,3-Dimethylphenol 6. 2,5-Dichlorophenol 2. 2,5-Dimethylphenol 7. 2,6-Dichlorophenol 3. 2,6-Dimethylphenol 8. 3,4-Dichlorophenol 4. 3,4-Dimethylphenol 9. 3,5-Dichlorophenol 5. 3,5-Dimethylphenol 10. 2,4-Dibromophenol
App
ID: 1
6297
Luna 3 µm PFP(2) SecurityGuard™ Cartridges (mm)
Part No. Dimensions (mm) /10pk
00B-4447-B0 50 x 2.0 AJ0-8326
00F-4447-B0 150 x 2.0 AJ0-8326
00B-4447-Y0 50 x 3.0 AJ0-8326
00F-4447-Y0 150 x 3.0 AJ0-8326
00B-4447-E0 50 x 4.6 AJ0-8327
00D-4447-E0 100 x 4.6 AJ0-8327
00F-4447-E0 150 x 4.6 AJ0-8327
00G-4447-E0 250 x 4.6 AJ0-8327
Luna 5 µm PFP(2) SecurityGuard™ Cartridges (mm)
Part No. Dimensions (mm) /10pk
00B-4448-B0 50 x 2.0 AJ0-8326
00F-4448-B0 150 x 2.0 AJ0-8326
00B-4448-Y0 50 x 3.0 AJ0-8326
00F-4448-Y0 150 x 3.0 AJ0-8326
00B-4448-E0 50 x 4.6 AJ0-8327
! co-elution
Luna 5 µm PFP(2) SecurityGuard™ Cartridges (mm)
Part No. Dimensions (mm) /10pk
00B-4448-P0-AX 50 x 21.2 AJ0-8377**
00D-4448-P0-AX 100 x 21.2 AJ0-8377**
00F-4448-P0-AX 150 x 21.2 AJ0-8377**
00G-4448-P0-AX 250 x 21.2 AJ0-8377**
00B-4448-U0-AX 50 x 30 AJ0-8378♦
00D-4448-U0-AX 100 x 30 AJ0-8378♦
00F-4448-U0-AX 150 x 30 AJ0-8378♦
00G-4448-U0-AX 250 x 30 AJ0-8378♦
for ID: 18.0 - 49.0 mm
PFP(2)
C18(2)
ORDERING INFORMATION SecurityGuard™ Cartridges require universal holder Part No.: KJ0-4282
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223♦PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8277
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CHIRAL StAtIOnARY PHASES
LUX™ Cellulose-2 • Unique enantioselectivity • Excellent for chiral screening • Complimentary selectivity with Lux™ Cellulose-1 • Pressure rated to 300 bar
Phenyl rings contribute to π- π interactions.
Lux™ Cellulose-2 uses the chiral selector cellulose tris(3-chloro-4-methylphenylcarbamate)
Electronegative atoms, such as oxygen, nitrogen, and chlorine are responsible for hydrogen bonding.
Chlorine atoms have strong electronegativity, contributing to dipole interactions.
Cellulose backbones form linear chains, creating various grooves and cavities which provide steric and inclusion interactions.
17349
0 2
2
1
4 6 8 min
0
200
400
mAU
17350
0 2
2
1
4 6 8 min
0
200
400
mAU
Mephenytoin
N
HN
O
CH3
H 3C
O
Lux™ Cellulose-2a = 1.83
*CHIRALCEL® OD-H®
a = 1.19
Ap
p ID
173
49A
pp
ID 1
7350
Column: Lux™ 5 µm Cellulose-2Dimensions: 250 x 4.6 mm ID
Part No.: 00G-4457-E0Mobile Phase: 0.1 % Diethylamine in Hexane /
0.1 % Diethylamine in Isopropanol (80:20)Flow Rate: 1 mL/minDetection: UV @ 220 nm
Temperature: AmbientSample: Mephenytoin
Column: *CHIRALCEL® 5 µm OD-H®
Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Hexane /
0.1 % Diethylamine in Isopropanol (80:20)Flow Rate: 1 mL/minDetection: UV @ 220 nm
Temperature: AmbientSample: Mephenytoin
*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.
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CHIRAL StAtIOnARY PHASES
LUX™ Cellulose-1 • Cost-effective replacement to *CHIRALCEL® OD-H®
• Broad enantioselectivity • Complimentary selectivity with Lux™ Cellulose-2 • Pressure rated to 300 bar
Lux™ Cellulose-1 uses the chiral selector cellulose tris(3,5-dimethylphenylcarbamate)
Phenyl rings contribute to π- π interactions.
Electronegative atoms, such as oxygen and nitrogen, are responsible for hydrogen bonding.
Cellulose backbones form linear chains, creating various grooves and cavities which provide steric interactions.
Homatropine
0 1 2 3 4 5 min
1
2
0
200
400
600
800
mAU
17378
0 1 2 3 4 5 min
1
2
0
200
400
600
800
mAU
17379
Lux™ Cellulose-1a = 1.82
*CHIRALCEL® OD-H®
a = 1.67
Ap
p ID
173
78A
pp
ID 1
7379
N+
O
O
O H
CH3
CH3
Column: Lux™ 5 µm Cellulose-1Dimensions: 250 x 4.6 mm ID
Part No.: 00G-4459-E0Mobile Phase: 0.1 % Diethylamine in Methanol /
0.1 % Diethylamine in Isopropanol (90:10)Flow Rate: 1 mL/minDetection: UV @ 220 nm
Temperature: AmbientSample: Homatropine
Column: *CHIRALCEL® 5 µm OD-H®
Dimensions: 250 x 4.6 mm IDMobile Phase: 0.1 % Diethylamine in Methanol /
0.1 % Diethylamine in Isopropanol (90:10)Flow Rate: 1 mL/minDetection: UV @ 220 nm
Temperature: AmbientSample: Homatropine
3 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)150 x 2.0 100 x 4.6 150 x 4.6 250 x 4.6 4 x 2.0* 4 x 3.0*
Phases /10pk /10pk
Lux Cellulose-1 00F-4458-B0 00D-4458-E0 00F-4458-E0 00G-4458-E0 AJ0-8402 AJ0-8403
Lux Cellulose-2 00F-4456-B0 00D-4456-E0 00F-4456-E0 00G-4456-E0 AJ0-8398 AJ0-8366
for ID: 2.0–3.0 mm 3.2–8.0 mm
5 μm Analytical Columns (mm) SecurityGuard™ Cartridges (mm)100 x 4.6 150 x 4.6 250 x 4.6 4 x 3.0*
Phases /10pk
Lux Cellulose-1 00D-4459-E0 00F-4459-E0 00G-4459-E0 AJ0-8403
Lux Cellulose-2 00D-4457-E0 00F-4457-E0 00G-4457-E0 AJ0-8366
for ID: 3.2–8.0 mm
5 μm Axia™ Packed Preparative Columns (mm) SecurityGuard™ Cartridges (mm)150 x 21.2 250 x 21.2 250 x 30 250 x 50 15 x 21.2** 15 x 30.0+
Phases /ea /ea
Lux Cellulose-1 00F-4459-P0-AX 00G-4459-P0-AX 00G-4459-U0-AX 00G-4459-V0-AX AJ0-8405 AJ0-8406
Lux Cellulose-2 00F-4457-P0-AX 00G-4457-P0-AX 00G-4457-U0-AX 00G-4457-V0-AX AJ0-8400 AJ0-8401
for ID: 18–29 mm 30–49 mm
Column Performance Check StandardPart No. Description Unit Price
AL0-8412 Chiral Test Mix No. 5 (Lux) ea
ORDERING INFORMATION
*CHIRALCEL and OD-H are registered trademarks of DAICEL Chemical Industries, Ltd. Comparative separations may not be representative of all applications.
*SecurityGuard Analytical Cartridges require holder, Part No. : KJ0-4282**PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8223+PREP SecurityGuard Cartridges require holder, Part No. : AJ0-8277
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Now Available in 150 & 250 mm Lengths - 21.2, 30, and 50 mm ID’s
tHE FUtURE OF PREPARAtIVE CHROMAtOGRAPHY
Compare LifetimeAn Axial Compression System in a High-Throughput Preparative Column!
Axia Packed Leading Competitive Preparative Column
Luna® 5 µm C18(2) Axia Packed Waters® XTerra® 5 µm Prep MS C18 OBD™
First Run 800th run First Run 120th run** Six columns tested. Best lifetime chosen for comparison.
Axia™
• Award winning Prep LC column technology • Significantly longer lifetime with patent-pending technology • Improved column-to-column reproducibility • Recover higher compound purity with analytical-like efficiency • Axia columns packed with high surface area media ideal for preparative chromatography
3
0
500
2 4 min
mAu
3
3
0
500
2
21 321
4 min
mAu
vs. Conditions: (same for both columns except where noted) Columns: Luna 5 µm C18(2) Axia Packed Waters XTerra 5 µm Prep MS C18 OBD Dimension: 50 x 21.2 mm (Luna) 50 x 19 mm (XTerra) Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 500 μL Flow Rate: 30 mL/min (Luna) 24 mL/min (XTerra)‡
Temperature: Ambient Detection: UV @ 254 nm Sample: 1. Triprolidine 1.6 mg 2. Methacycline 16 mg 3. Amitriptyline 5.25 mg ‡ Same linear velocity
Reproducible Column-to-Column Efficiency
Reproducible Column-to-Column Peak Asymmetry
Average Efficiency (N) with Synergi 4 µm Hydro-RP 100 x 21.2 mm
Average Peak Asymmetry with Gemini 5 µm C18 50 x 21.2 mm
27 % Improved Avg. Efficiency 13 % Improved Avg. Peak Shape
Expect an Excellent Axia Column. Every Time. Consistent Quality. Column-to-Column. Batch-to-Batch.
: Visit www.axiaprep.com to view the Award winning technology in action.Conventional
Slurry PackingAxia Packed
Hydraulic Piston Compression Packing
ConventionalSlurry Packing
Axia PackedHydraulic Piston
Compression Packing
Effic
ienc
y (N
)
Peak
Asy
mm
etry
1
1.1
1.2
1.3
1.4
40000
60000
80000
100000
120000
11.8
2.5
5.2
9.2
% RSD % RSDAxia Hydraulic Piston Compression (Patent Pending)
With the Axia—hydraulic piston compression technology—the piston compresses the bed and pressure is never released (no chance of media loss). The optimum slurry amount and packing pressure are automated for reproducible packing control. And in the final product, the piston remains in place, yielding an excellent column with extended lifetime and consistent peak shape. Every time.
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tHE FUtURE OF PREPARAtIVE CHROMAtOGRAPHY
For more information about SecurityGuard see pp. 12-13
Conditions: (same for both columns except where noted) Columns: Luna 5 µm C18(2) Axia Packed Waters XTerra 5 µm Prep MS C18 OBD Dimension: 50 x 21.2 mm (Luna) 50 x 19 mm (XTerra) Mobile Phase: A: 0.5 % TFA in Water B: 0.5 % TFA in Acetonitrile Gradient: Linear 95:5 (A/B) to 5:95 (A/B) over 7 min, hold 3 min Injection Volume: 500 μL Flow Rate: 30 mL/min (Luna) 24 mL/min (XTerra)‡
Temperature: Ambient Detection: UV @ 254 nm Sample: 1. Triprolidine 1.6 mg 2. Methacycline 16 mg 3. Amitriptyline 5.25 mg ‡ Same linear velocity
50 x 21.2 mm 100 x 21.2 mm 150 x 21.2 mm 250 x 21.2 mm 50 x 30 mm 100 x 30 mmGemini 5 µm C18 00B-4435-P0-AX 00D-4435-P0-AX 00F-4435-P0-AX 00G-4435-P0-AX 00B-4435-U0-AX 00D-4435-U0-AXGemini 10 µm C18 — — — 00G-4436-P0-AX — —Gemini 5 µm C6-Phenyl 00B-4444-P0-AX 00D-4444-P0-AX 00F-4444-P0-AX 00G-4444-P0-AX 00B-4444-U0-AX 00D-4444-U0-AXGemini-NX 5 µm C18 00B-4454-P0-AX 00D-4454-P0-AX 00F-4454-P0-AX 00G-4454-P0-AX 00B-4454-U0-AX 00D-4454-U0-AXGemini-NX 10 µm C18 — — — 00G-4455-P0-AX — —
Luna 5 µm C18(2) 00B-4252-P0-AX 00D-4252-P0-AX 00F-4252-P0-AX 00G-4252-P0-AX 00B-4252-U0-AX 00D-4252-U0-AXLuna 10 µm C18(2) — — — 00G-4253-P0-AX — —Luna 5 µm C8(2) 00B-4249-P0-AX 00D-4249-P0-AX 00F-4249-P0-AX 00G-4249-P0-AX 00B-4249-U0-AX 00D-4249-U0-AXLuna 10 µm C8(2) — — — 00G-4250-P0-AX — —Luna 5 µm CN 00B-4255-P0-AX — 00F-4255-P0-AX 00G-4255-P0-AX — 00D-4255-U0-AXLuna 5 µm NH2 — 00D-4378-P0-AX 00F-4378-P0-AX 00G-4378-P0-AX — 00D-4378-U0-AXLuna 5 µm PFP(2) 00B-4448-P0-AX 00D-4448-P0-AX 00F-4448-P0-AX 00G-4448-P0-AX 00B-4448-U0-AX 00D-4448-U0-AXLuna 5 µm Phenyl-Hexyl 00B-4257-P0-AX 00D-4257-P0-AX 00F-4257-P0-AX 00G-4257-P0-AX 00B-4257-U0-AX 00D-4257-U0-AXLuna 10 µm Phenyl-Hexyl — — — 00G-4285-P0-AX — —Luna 5 µm Silica(2) 00B-4274-P0-AX 00D-4274-P0-AX 00F-4274-P0-AX 00G-4274-P0-AX 00B-4274-U0-AX 00D-4274-U0-AXLuna 10 µm Silica(2) — — — 00G-4091-P0-AX — —
Lux 5 µm Cellulose-1 — 00D-4459-P0-AX — 00G-4459-P0-AX — —Lux 5 µm Cellulose-2 — 00D-4457-P0-AX — 00G-4457-P0-AX — —
Synergi 4 µm Max-RP 00B-4337-P0-AX 00D-4337-P0-AX 00F-4337-P0-AX 00G-4337-P0-AX 00B-4337-U0-AX 00D-4337-U0-AXSynergi 10 µm Max-RP — — — 00G-4350-P0-AX — —Synergi 4 µm Hydro-RP 00B-4375-P0-AX 00D-4375-P0-AX 00F-4375-P0-AX 00G-4375-P0-AX 00B-4375-U0-AX 00D-4375-U0-AXSynergi 10 µm Hydro-RP — — — 00G-4376-P0-AX — —Synergi 4 µm Polar-RP 00B-4336-P0-AX 00D-4336-P0-AX 00F-4336-P0-AX 00G-4336-P0-AX 00B-4336-U0-AX 00D-4336-U0-AXSynergi 10 µm Polar-RP — — — 00G-4351-P0-AX — —Synergi 4 µm Fusion-RP 00B-4424-P0-AX 00D-4424-P0-AX — — — —
150 x 30 mm 250 x 30 mm 50 x 50 mm 100 x 50 mm 150 x 50 mm 250 x 50 mm 21.2* 30.0**Gemini 5 µm C18 00F-4435-U0-AX 00G-4435-U0-AX 00B-4435-V0-AX — — — AJ0-7839 AJ0-8301Gemini 10 µm C18 — 00G-4436-U0-AX — 00D-4436-V0-AX 00F-4436-V0-AX 00G-4436-V0-AX AJ0-7839 AJ0-8301Gemini 5 µm C6-Phenyl 00F-4444-U0-AX 00G-4444-U0-AX — — — — AJ0-7841 AJ0-8303Gemini-NX 5 µm C18 00F-4454-U0-AX 00G-4454-U0-AX 00B-4454-V0-AX — — — AJ0-8370 AJ0-8371Gemini-NX 10 µm C18 — 00G-4455-U0-AX — 00D-4455-V0-AX 00F-4455-V0-AX 00G-4455-V0-AX AJ0-8370 AJ0-8371
Luna 5 µm C18(2) 00F-4252-U0-AX 00G-4252-U0-AX 00B-4252-V0-AX — — — AJ0-7839 AJ0-8301Luna 10 µm C18(2) — 00G-4253-U0-AX — 00D-4253-V0-AX — 00G-4253-V0-AX AJ0-7839 AJ0-8301Luna 5 µm C8(2) 00F-4249-U0-AX 00G-4249-U0-AX 00B-4249-V0-AX — — — AJ0-7840 AJ0-8302Luna 10 µm C8(2) — 00G-4250-U0-AX — — — 00G-4250-V0-AX AJ0-7840 AJ0-8302Luna 5 µm CN 00F-4255-U0-AX 00G-4255-U0-AX — — — — AJ0-7840 AJ0-8302Luna 5 µm NH2 00F-4378-U0-AX 00G-4378-U0-AX — — — — AJ0-8162 AJ0-8309Luna 5 µm PFP(2) 00F-4448-U0-AX 00G-4448-U0-AX — — — — AJ0-8162 AJ0-8309Luna 5 µm Phenyl-Hexyl 00F-4257-U0-AX 00G-4257-U0-AX — — — — AJ0-7841 AJ0-8303Luna 5 µm Silica(2) 00F-4274-U0-AX 00G-4274-U0-AX — — — — AJ0-7229 AJ0-8312Luna 10 µm Silica(2) — 00G-4091-U0-AX — — — 00G-4091-V0-AX AJ0-7229 AJ0-8312
Lux 5 µm Cellulose-1 — 00G-4459-U0-AX — — — 00G-4459-V0-AX AJ0-8405 AJ0-8406Lux 5 µm Cellulose-2 — 00G-4457-U0-AX — — — 00G-4457-V0-AX AJ0-8400 AJ0-8401
*PREP SecurityGuard™ Cartridges require holder, Part No.: AJO-8223**PREP SecurityGuard™ Cartridges require holder, Part No.: AJO-8277
ORDERING INFORMATION
21.2* 30.0**AJ0-7839 AJ0-8301AJ0-7839 AJ0-8301AJ0-7841 AJ0-8303AJ0-8370 AJ0-8371AJ0-8370 AJ0-8371
AJ0-7839 AJ0-8301AJ0-7839 AJ0-8301AJ0-7840 AJ0-8302AJ0-7840 AJ0-8302AJ0-7840 AJ0-8302AJ0-8162 AJ0-8309AJ0-8162 AJ0-8309AJ0-7841 AJ0-8303AJ0-7841 AJ0-8303AJ0-7229 AJ0-8312AJ0-7229 AJ0-8312
AJ0-8405 AJ0-8406AJ0-8400 AJ0-8401
AJ0-7842 AJ0-8304AJ0-7842 AJ0-8304AJ0-7843 AJ0-8305AJ0-7843 AJ0-8305AJ0-7845 AJ0-8307AJ0-7845 AJ0-8307
AJ0-7844 AJ0-8306
SecurityGuard™ Cartridges (mm)
Additional Axia dimensions available-please contact your authorized Phenomenex distributor.
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Clarity® BioSeparations • Extensive product portfolio for synthetic DNA/RNA purification & characterization • Purify several types of synthetic oligonucleotides, including DNA, RNA/RNAi, longmers, dye-labeled, & modified • Recoveries and purities ranging from 70 – 95 % depending on solution selected • High-throughput, high purity solution available with R&D 100 Award winning Clarity QSP™
YOUR PARtnER In OLIGOnUCLEOtIDE PURIFICAtIOn
Clarity Desalting • Quick, simple removal of salt and excess reagent • Reversed phase desalting method results in low conductivity • 80 % typical recovery of target synthetic DNA/RNA sequence • Economical & disposable format
Clarity Desalting TubesPhase 200 mg/3 mL* 500 mg/3 mL**
50/box 50/box
C18 8B-S041-FBJ 8B-S041-HBJ
* For 200 µmole synthesis** For 1 µmole synthesis
Clarity QSP Clarity Oligo-RP Clarity Oligo-WAX Clarity Desalting
Format(s) 50 mg/ 96-well plate
150 mg/ 3 mL cartridges 5 g/ 60 mL cartridges
Analytical, semi-prep, & preparative HPLC columns
Analytical, semi-prep, & preparative HPLC columns
200 mg/ 3 mL tubes 500 mg/ 3 mL tubes
Equipment Required VVacuum manifold or automated liquid handling system HPLC system HPLC OR FPLC systems Vacuum manifold
Purification Time ~8 minutes per cartridge
~45 minutes per well platee15 – 30 minutes 3 20 – 60 minutes 3 15 minutes
Oligo Length ≤ 100 nt ≤ 60 nt ≤ 80 nt ≤ 60 nt
Synthesis Scale Load 1 nmole – 50 µmole 1 nmole – 50 µmole 1 nmole – 50 µmole ≤ 1 µmole
Typical Purity 90 – 95 % 1 90 – 95 % 4 ≥ 90 % ~70 % 4
Typical Recovery Yield ~90 % 2 ~70 % ≥ 90 % ~80 %
1. Purity value based on ion-exchange chromatography and capillary electrophoresis 2. OD260 used for quantitation 3. Time range is dependent on dimension of HPLC column and flow rate4. Purity value based on reversed phase chromatography
30
ORDERING INFORMATION
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Clarity® QSP™ Cartridges and Well Plates • High-throughput, high purity cartridge & 96-well plate solutions for synthetic RNA & DNA • 90 % typical purities and yields • Simple 3-step, economical process delivers highly purified DNA/RNA in minutes • Formats easily amenable to parallel processing equipment • No toxic ion pairing reagents to interfere with downstream applications
YOUR PARtnER In OLIGOnUCLEOtIDE PURIFICAtIOn
Detritylated Failure SequencesTrityl GroupFull Length Target Oligo
Full Length Trityl - On OligoImpurityN-1 Sequence
STEP 3
Elution of Target Oligo
Organic M
ixture
Elute target oligopH buffered solutions used to
maintain safe pH for oligo;select elution buffer based on
downstream requirements
STEP 2
Detritylate
DC
A
DetritylateLess than 2 %
depurination observed.A faint orange band
will appear at top halfof cartridge indicating
DMT retention
STEP 1
Loading of Crude Oligo
Load crude oligo cocktailAll trityl-off impurities flowdirectly through; no wash
required
Part No. Description Unit PriceFormats
8E-S102-DGB Clarity QSP Well Plate 50 mg/well 1/box8B-S102-SBJ Clarity QSP Cartridge 150 mg/3 mL 50/box8B-S042-LFF Clarity QSP Cartridge 5 g/60 mL 16/box
Buffers*
AL0-8279 Clarity QSP DNA Loading Buffer 100 mL eaAL0-8280 Clarity QSP DNA Loading Buffer 1 L eaAL0-8281 Clarity QSP RNA-TDBMS Loading Buffer 100 mL eaAL0-8282 Clarity QSP RNA-TDBMS Loading Buffer 1 L eaAH0-7858 Clarity Nuclease Free Water 1 L ea
Accessories
AH0-6024 24-Position Vacuum Manifold Complete Set eaAH0-7284 96-Well Plate Manifold Acrylic eaAH0-7194 96 Square Well Collection Plate, Conical V-bottom 2 mL/well (Polypropylene) 50/pkAH0-7408 Solvent Waste Reservoir Tray for Well Plate Manifolds 25/pkAH0-7195 96-Well Pierceable Sealing Mat Square Well 50/pk
ORDERING INFORMATION
* RNA-TOM loading buffer available upon request.
2008 R&D 100 Award Recipient
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Material Characteristics
Clarity® Oligo-RP™
• Easily purify N-1 failure sequences from target oligo with > 90 % purities • MS-friendly protocol for characterization of synthetic DNA and RNA • Analytical columns through bulk material available to support various loading capacities • Excellent column for reversed phase HPLC quality control testing
YOUR PARtnER In OLIGOnUCLEOtIDE PURIFICAtIOn
Excellent selectivity characteristics of Clarity oligo-rP allow baseline separation of failure N-1 sequences from target N sequences.
min 0 5 10 15 20 25 30 35 40
mAU
0
10
20
30
40
50
21 nt20 nt
15935
App
ID 1
5935
Column: Clarity 3 μm Oligo-RP C18Dimensions: 50 x 4.6 mm
Part No.: 00B-4441-E0Mobile Phase: A: 10 mM TPAC
B: MethanolGradient: A/B (75:25) to A/B (55:45) in 80 min (50
min run time)Flow Rate: 1 mL/minDetection: UV @ 260 nm
Sample: 1. 20 nt RNA with sequence CUGUAAUCUCUUGUCUATT (2.5 μg) 2. 21 nt RNA with sequence UCUGUAAUCUCUUGUCUATT (2.5 μg)
Clarity® Oligo-RP™
Particle Support TWIN™ (silica organic composite)Bonded Phase C18Particle Size 3, 5, and 10 µmPore Size 110 ÅSurface Area 375 m2/gpH Stability 1.0 – 12.0 pH Effective Range 1.0 – 12.0
RNA Purification of Failure N-1 Sequence from Target N Sequence
3 µm Columns (mm)Phases 50 x 1.0 100 x 1.0 50 x 2.0 100 x 2.0 150 x 2.0Oligo-RP 00B-4441-A0 00D-4441-A0 00B-4441-B0 00D-4441-B0 00F-4441-B0
Phases 50 x 4.6 100 x 4.6 150 x 4.6 50 x 10.0Oligo-RP 00B-4441-E0 00D-4441-E0 00F-4441-E0 00B-4441-N0
5 µm Columns (mm)Phases 50 x 1.0 150 x 2.0 50 x 4.6 150 x 4.6 250 x 4.6Oligo-RP 00B-4442-A0 00F-4442-B0 00B-4442-E0 00F-4442-E0 00G-4442-E0
Phases 50 x 10.0 100 x 10.0 250 x 10.0 250 x 21.2 250 x 30.0
Oligo-RP 00B-4442-N0 00D-4442-N0 00G-4442-N0 00G-4442-P0-AX 00G-4442-U0
10 µm Columns (mm)Phases 150 x 4.6 150 x 10.0 150 x 30.0 150 x 50.0Oligo-RP 00F-4445-E0 00F-4445-N0 00F-4445-U0 00F-4445-V0
Phases 250 x 10.0 250 x 21.2 250 x 50.0
Oligo-RP 00G-4445-N0 00G-4445-P0-AX 00G-4445-V0
*SecurityGuard Analytical Cartidges require holder, Part No.: KJ0-4282 ‡SecurityGuard Analytical Cartidges require holder, Part No.: AJ0-7220 **SecurityGuard Analytical Cartidges require holder, Part No.: AJ0-8223
AJ0-8134 SecurityGuard™ Cartridges Clarity Oligo-RP 4 x 2.0 mm* 10/Pk 2.0 - 3.0 mmAJ0-8135 SecurityGuard Cartridges Clarity Oligo-RP 4 x 3.0 mm* 10/Pk 3.2 - 8.0 mmAJ0-8136 SecurityGuard Semi-Prep Cartridges Clarity Oligo-RP 10 x 10 mm‡ 3/Pk 9 - 16 mmAJ0-8210 SecurityGuard PREP Cartridge Clarity Oligo-RP 15 x 21.2 mm ID** Ea 18 - 29 mmAJ0-8310 SecurityGuard PREP Cartridge Clarity Oligo-RP 15 x 30 mm ID** Ea 30 - 49 mm
Part No. Description Unit For ID
GUARD CARTRIDGE SYSTEM
ORDERING INFORMATION
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For more information about SecurityGuard see pp. 12-13
YOUR PARtnER In OLIGOnUCLEOtIDE PURIFICAtIOn
Clarity® Oligo-WAX™
• Higher loading capacity ion-exchange, silica material for prep/process purifications • Better fractionation of closely eluting compounds resulting in higher purity • Increase productivity by running at higher flow rates and pressures
Two purification runs were performed on each column with fractional QC being taken after each run. Passing fractions from the two purification runs were combined into one pooled lot for each column. That pooled lot was then divided equally and run through a Clarity desalting tube. Final OD’s and QC were taken after desalting, including ESI, CE, and conductivity. The purity and resolution of Clarity Oligo-WAX was considerably better than SOURCE 15Q. Though SOURCE had a slightly higher recovery of full length oligo, it was not a wide enough margin to offset the purity advantage.
GE™ Healthcare SOURCE™ 15Q 150 x 10 mm (self-packed) Clarity Oligo-WAX 10 µm 150 x 10 mm (pre-packed)
• Final Purity = 88.8 %, N-1 = 2.0 % • Final amount = 205.9 OD’s• Recovery of full-length product = 28.9 % • Conductivity = 200 µS/cm
• Final Purity = 95.1 %, N-1 = 1.3 % • Final amount = 188.9 OD’s• Recovery of full-length product = 28.4 % • Conductivity = 151 µS/cm
Data courtesy of a large, Iowa-based oligo manufacturer. Comparative separations may not be representative of all applications.
* SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJ0-4282 ‡ Semi-prep SecurityGuard™ require universal holder Part No.: AJ0-7220 ** Prep SecirutyGuard™ require universal holder Part No.: AJ0-8223 u Prep SecirutyGuard™ require universal holder Part No.: AJ0-8277
CE Purity Analysis of Ion-Exchange Purification
Clarity Oligo-WAX™ HPLC Columns SecurityGuard™ Cartridges (mm)
10 µm Minibore, Analytical, Semi-Prep & Prep Columns (mm)
Phase 100 x 4.6 150 x 4.6 150 x 10.0 150 x 21.2 150 x 30.0 250 x 10.0 4 x 3.0* 10 x 10‡
/10 pk /3 pkOligo-WAX 00D-4451-E0 00F-4451-E0 00F-4451-N0 00F-4451-P0 00F-4451-U0 00G-4451-N0 AJ0-8324 AJ0-8325
for ID: 3.2-8.0 mm 9-16 mm
ORDERING INFORMATION
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RP-HPLC PROtEIn/PEPtIDE CHARACtERIZAtIOn AnD PURIFICAtIOn
Jupiter® 300 300 Å columns designed to analyze and purify intact proteins (> 10,000 MW)
• C18, C5, and C4 bonded phases • 1.5 – 10 pH stability for method ruggedness and easy protein removal • Direct scale-up to preparative and bulk materials
Jupiter® Proteo 90 Å columns engineered for increased peak capacity and resolution of peptide maps/peptide separations
(< 10,000 MW)
• Novel C12 bonded phase for excellent selectivity • Identify post-translational modifications • Capillary columns available for increased sensitivity
a-Chymotrypsin Tryptic Digest on Jupiter Proteo
Column: Jupiter 4 μm Proteo 90 ÅDimensions: 150 x 0.5 mmPart No.: 00F-4396-AFMobile Phase: A: 0.01 % TFA in Water B: 0.008 % TFA in Acetonitrile
Gradient: A/B (95:5) for 5 min then A/B (55:45) in 45 min Switch to A/B (95:5) in 1 sec then hold for 10 min Flow Rate: 15 µL/minTemperature: 40 °CDetection: UV @ 210 nmSample: a - Chymotrypsin tryptic digest
App ID 14336
0 10 20 30 40 min
for ID: 2.0-3.0 mm
3 μm, 4 μm & 5 μm microbore and minibore Columns (mm) SecurityGuard™ Cartridges (mm) 50 x 1.0 150 x 1.0 250 x 1.0 50 x 2.0 150 x 2.0 250 x 2.0 4 x 2.0*Phases /10pk5 μm C4 300 Å I 00B-4167-A0 I 00F-4167-A0 I 00G-4167-A0 I 00B-4167-B0 I 00F-4167-B0 I 00G-4167-B0 AJ0-43295 μm C5 300 Å 00B-4052-A0 00F-4052-A0 00G-4052-A0 00B-4052-B0 00F-4052-B0 00G-4052-B0 AJ0-43265 μm C18 300 Å 00B-4053-A0 00F-4053-A0 00G-4053-A0 00B-4053-B0 00F-4053-B0 00G-4053-B0 AJ0-43204 μm Proteo 90 Å 00B-4396-A0 00F-4396-A0 00G-4396-A0 00B-4396-B0 00F-4396-B0 00G-4396-B0 AJO-60733 μm C18 300 Å — — — 00B-4263-B0 00F-4263-B0 — AJO-4320
4 μm & 5 μm Capillary Columns (mm) 50 x 0.30 150 x 0.30 250 x 0.30 50 x 0.50 150 x 0.50 250 x 0.50 Phases
5 μm C4 300 Å I 00B-4167-AC I 00F-4167-AC I 00G-4167-AC I 00B-4167-AF I 00F-4167-AF I —5 μm C18 300 Å 00B-4053-AC 00F-4053-AC 00G-4053-AC 00B-4053-AF 00F-4053-AF 00G-4053-AF4 μm Proteo 90 Å 00B-4396-AC 00F-4396-AC 00G-4396-AC 00B-4396-AF 00F-4396-AF 00G-4396-AF
ORDERING INFORMATION
18-29 mmfor ID: 3.2-8.0 mm 9-16 mm
*SecurityGuard™ Analytical Cartridges require holder, Part No.: KJ0-4282
‡SemiPrep SecurityGuard™ Cartridges require holder, Part No.: AJ0-7220
**PREP SecurityGuard™ Cartridges require holder, Part No.: AJ0-8223
Large Proteins on Jupiter 300 C4Column: Jupiter 300 5 µm C4Dimensions: 150 x 4.6mmPart No.: 00F-4167-E0Mobile Phase: A) 0.1% TFA in Water B) 0.1% TFA in 90% AcetonitrileGradient: A/B (95:5) to A/B (20:80) in 20 minutesFlow Rate: 1 mL/minTemperature: 35 °CDetection: UV @ 280 nmInjection Volume: 20 µLSample: Large Proteins 1. Bovine Serum Albumin (66 kDa) 2. Glutamic Dehydrogenase (56 kDa) 3. Ovalbumin (44 kDa) 4. Phosphorylase b (97 kDa)
min0 2.5 5 7.5 10 12.5 15 17.5 20
mAU
0
10
20
30
40
50
60
70
801
2
3
4 App ID 14761
10 μm and 15 μm also available-please contact your authorized Phenomenex distributor.
3 μm, 4 μm & 5 μm analytical and Preparative Columns (mm) SecurityGuard™ Cartridges (mm)
50 x 4.6 150 x 4.6 250 x 4.6 250 x 10 250 x 21.2 4 x 3.0* 10 x 10‡ 15 x 21.2**Phases
5 μm C4 300 Å I 00B-4167-E0 I 00F-4167-E0 I 00G-4167-E0 I 00G-4167-N0 I 00G-4167-P0 AJO-4330 I AJO-7225 I AJO-7231 5 μm C5 300 Å 00B-4052-E0 00F-4052-E0 00G-4052-E0 00G-4052-N0 00G-4052-P0 AJO-4327 AJO-7371 —5 μm C18 300 Å 00B-4053-E0 00F-4053-E0 00G-4053-E0 00G-4053-N0 00G-4053-P0 AJO-4321 AJO-7224 AJO-72304 μm Proteo 90 Å 00B-4396-E0 00F-4396-E0 00G-4396-E0 00G-4396-N0 00G-4396-P0-AX AJO-6074 AJO-7275 AJO-78423 μm C18 300 Å — 00F-4263-E0 00G-4263-E0 — — AJO-4321 — —
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PROtEIn/PEPtIDE SIZE EXCLUSIOn/FILtRAtIOn
For more information about SecurityGuard see pp. 12-13
BioSep™ SEC-SEconomical protein/peptide size exclusion/filtration
• Characterize aggregates, dimers, and enzyme activity • Purify protein/peptide mixtures with wide molecular weight distribution • Excellent stability, reproducibility, & loading capacity
BioSep™ SEC-S2000Tosoh Bioscience
TSK-GEL® 2000SW
Bio-Rad
Bio-Sil® SEC 125
Agilent Technologies
ZORBAX® GF-250
BioSep™ SEC-S3000Tosoh Bioscience
TSK-GEL® 3000SW
Bio-Rad
Bio-Sil® SEC 250
Agilent Technologies
ZORBAX® GF-450
BioSep™ SEC-S4000Tosoh Bioscience
TSK-GEL® 4000SW
Bio-Rad
Bio-Sil® SEC 400
Agilent Technologies
*Bio-Rad protein standard was reconstituted according to manufacturer's instructions.
4 6 8 10 12 14
6
2
1 3
45
7
min
Protein Standards Column: BioSep-SEC-S3000
Dimensions: 300 x 7.8 mm
Part No.: 00H-2146-K0
Mobile Phase: 50 mM PO4 buffer, pH 6.8
Flow Rate: 1 mL/min
Detection: UV @ 280 nm
Sample: Bio-Rad protein standard, *20 µL injected
1. High MW Aggregates
2. Thyroglobulin
3. IgA
4. IgG
5. Ovalbumin
6. Myoglobin
7. Cyanocobalamin
App ID 5178
ORDERING INFORMATION
Stainless Steel Columns (mm): narrow bore analytical Preparative
300 x 4.6 300 x 7.8 600 x 7.8 300 x 21.2 600 x 21.2 Phases2000 I 00H-2145-E0 00H-2145-K0 I 00K-2145-K0 00H-2145-P0 I 00K-2145-P0
3000 00H-2146-E0 00H-2146-K0 00K-2146-K0 00H-2146-P0 00K-2146-P0
4000 00H-2147-E0 00H-2147-K0 00K-2147-K0 00H-2147-P0 00K-2147-P0
Stainless Steel Guards (mm): SecurityGuard™
narrow bore Express analytical Preparative Cartridges 30 x 4.6 35 x 7.8 75 x 7.8 60 x 21.2 4 x 3.0 mm Phases
2000 I 03A-2145-E0 03Q-2145-K0 03C-2145-K0 03R-2145-P0 AJ0-4487
3000 03A-2146-E0 03Q-2146-K0 03C-2146-K0 03R-2146-P0 AJ0-4488
4000 03A-2147-E0 03Q-2147-K0 03C-2147-K0 03R-2147-P0 AJ0-4489
SecurityGuard™Cartridges require universal holder Part No.: KJO-4282
for ID: 3.2-8.0 mm
/10pk
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Acid Amino Analysis using EZ:faast™
• Analyze over 60 free amino acids and related compounds • Sample preparation and derivatization completed in 7 minutes • No plasma or urea removal required • Chromatographic analysis is complete in less than 8 minutes • Great sample purity and improved reproducibility • One of the most economical amino acid analysis products with reagents and supplies for the analysis of 384 samples • Compatible with GC/FID, GC/MS and LC/MS systems
Amino Acid Standards Amino Acids in Human SerumApp ID 14168 App ID 14169
Kit: EZ:faast GC/FID Free (Physiological) Amino Acid Kit Part No.: KG0-7165 Injection: Split 1:15 @ 250 °C, 2.5 µL Carrier Gas: Helium 1.5 mL/minute (60 kPa) @ 110 °CPressure Rise: 6 kPa/min Oven Program: 30 °C/min from 110 °C to 320 °C, hold at 320 °C for 1 minute Detector: FID @ 320 °C Sample: Equimolar mixture of derivatized amino acid standards (10 nmol each) 1. Alanine 18. a-Aminoadipic acid 2. Glycine 19. Cysteine 3. a-Aminobutyric acid 20. a-Aminopimelic acid 4. Valine 21. Glutamine 5. b-Aminoisobutyric acid 22. Homocysteine 6. Norvaline 23. Ornithine 7. Leucine 24. Glycine-proline (dipeptide) 8. Isoleucine 25. Lysine 9. Threonine 26. Histidine 10. Serine 27. Hydroxylysine (2 isomers) 11. Proline 28. Tyrosine 12. Asparagine 29. Proline-hydroxyproline (dipeptide) 13. Aspartic acid 30. Tryptophan 14. Methionine 31. Cystathionine 15. 4-Hydroxyproline 32. Cystine 16. Glutamic acid 33. Homocystine 17. Phenylalanine
Kit: EZ:faast GC/FID Free (Physiological) Amino Acid Kit Part No.: KG0-7165 Injection: Split 1:15 @ 250 °C, 2.5 µL Carrier Gas: Helium 1.5 mL/minute (60 kPa) @ 110 °C Pressure Rise: 6 kPa/min Oven Program: 30 °C/min from 110 °C to 320 °C, hold at 320 °C for 1 minute Detector: FID @ 320 °C Sample: Derivatized amino acids in human serum (0.1 mL). Norvaline is the internal standard added at a concentration of 200 µmol/L 1. Alanine 13. Methionine 2. Glycine 14. 4-Hydroxyproline 3. a-Aminobutyric acid 15. Glutamic acid 4. Valine 16. Phenylalanine 5. Norvaline 17. Glutamine 6. Leucine 18. Ornithine 7. Isoleucine 19. Lysine 8. Threonine 20. Histidine 9. Serine 21. Tyrosine 10. Proline 22. Tryptophan 11. Asparagine 23. Cystine 12. Aspartic acid
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15 MInUtE AMInO ACID AnALYSIS
ORDERING INFORMATION Amino Acid Analysis KitsEach kit includes: ZB-AAA GC column, or AAA LC column, sample prep and derivatization reagents, sample prep vials, AA standards, SPE pipette tips, vial rack, autosampler vials with inserts with MS kits, and microdispenser.
Part No. Description Unit Price
KG0-7165 GC/FID Free (Physiological) Amino Acid Analysis Kit ea
KG0-7166 GC/MS Free (Physiological) Amino Acid Analysis Kit ea
KG0-7167 GC/FID Protein Hydrolysate Kit ea
KG0-7168 GC/MS Protein Hydrolysate Kit ea
KH0-7337 LC/MS Free (Physiological) Amino Acids Kit with 250 x 2.0 mm column ea
KH0-7338 LC/MS Free (Physiological) Amino Acids Kit with 250 x 3.0 mm column ea
KH0-7339 LC/MS Protein Hydrolysates Kit with 250 x 2.0 mm column ea
KH0-7340 LC/MS Protein Hydrolysates Kit with 250 x 3.0 mm column ea
– EZ:faast is in compliance with European Directive 98/79/EC for In Vitro Diagnostics.
Phenomenex | Web: www.phenomenex.com
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Column: Rezex ROA-Organic AcidDimensions: 150 x 7.8 mm
Part No.: 00F-0138-K0Mobile Phase: 0.005 N Sulfuric Acid
Flow Rate: 0.6 mL/minDetection: RI @ 40 °C
Temperature: 60 °CSystem: Shimadzu™ Prominence™ LC-20A SystemSample: 1. Dp4+ 5. Lactic Acid
2. Dp3 6. Glycerol3. Maltose 7. Acetic Acid4. Glucose 8. Ethanol
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FOOD AnD BEVERAGE AnALYSIS
Rezex™ HPLC Columns for Carbohydrate, Oligosaccharide, and Organic Acid Analysis • Analyze samples from foods, beverages, fermentation broths, and other complex matrices • Fast and easy bioethanol analysis • Recommended alternative to Bio-Rad Aminex®, Waters Sugar-Pak™, and Supelco Supelcogel™
Sugars
Baseline Separation of Critical Sample ComponentsDue to improved resolution
vs.Sugars14977 & 14978
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Rezex RCM
APP ID 14977
Great Peak Shape
vs.Sugars14977 & 14978
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Bio-Rad AminexHPX-87C
APP ID 14978
ORDERING INFORMATION Columns Guards SecurityGuard™Cartridges (mm)
4 x 3.0 Cross Ionic Description Part No. Linkage Form Size (mm) Price Part No. Size (mm) Price /10pk
RCM-Monosaccharide I 00H-0130-K0 I 8 % I Calcium I 300 x 7.8 I 03B-0130-K0 I 50 x 7.8 I AJ0-4493
RHM- Monosaccharide 00H-0132-K0 8 % Hydrogen 300 x 7.8 03B-0132-K0 50 x 7.8 AJ0-4490
RAM-Carbohydrate 00H-0131-K0 8 % Silver 300 x 7.8 — — AJ0-4491
RSO-Oligosaccharide 00P-0133-N0 4 % Silver 200 x 10.0 03R-0133-N0 60 x 10.0 AJ0-4491
RNO-Oligosaccharide 00P-0137-N0 4 % Sodium 200 x 10.0 03R-0137-N0 60 x 10.0 —
RPM-Monosaccharide 00H-0135-K0 8 % Lead 300 x 7.8 03B-0135-K0 50 x 7.8 AJ0-4492 (for USP procedure) 00D-0135-K0 8 % Lead 100 x 7.8 03B-0135-K0 50 x 7.8 AJ0-4492
RNM-Carbohydrate 00H-0136-K0 8 % Sodium 300 x 7.8 03B-0136-K0 50 x 7.8 —
ROA-Organic Acid 00H-0138-K0 8 % Hydrogen 300 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490
ROA-Organic Acid 00F-0138-K0 8 % Hydrogen 150 x 7.8 03B-0138-K0 50 x 7.8 AJ0-4490
RKP-Potassium 00H-3252-K0 8 % Potassium 300 x 7.8 — — —
RFQ-Fast Acid 00D-0223-K0 8 % Hydrogen 100 x 7.8 03B-0223-K0 50 x 7.8 AJ0-4490
RCU-USP Sugar Alcohols Column 00G-0130-D0 8 % Calcium 250 x 4.0 03A-0130-D0 30 x 4.0 AJ0-4493for ID: 3.2-8.0 mm
SecurityGuard™ Analytical Cartridges require universal holder Part No.: KJO-4282
Conditions for all columns:Dimensions: 300 x 7.8 mm
Mobile Phase: Water
Flow Rate: 0.6 mL/min
Detection: ELSD
Temperature: 80 °C
Sample: 1. Sucrose 2. Glucose 3. Galactose 4. Fructose 5. Sorbitol
Bioethanol Fermentation Monitoring
Bioethanol Analysis
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