Tab

le 5

. C

hara

cter

isti

cs o

f th

e C

omm

on H

PL

C S

epar

atio

n Sy

stem

s (c

olum

ns)

Nam

e C

hem

ical

nat

ure/

P

ore·

size

P

arti

cle

Par

ticl

e pH

·ran

ge/

Solv

ents

as

func

tion

al g

roup

s sh

ape

size

, ~m

reac

tion

el

uent

I. A

dsor

ptio

n Si

lica

gel

4-40

0 nm

ro

und

or

3-12

no

n po

lar

Ace

toni

tril

e,

Chr

omat

ogra

phy

irre

gula

r be

nzen

e, c

hlor

o-(N

orm

al p

hase

) Si

40-

Si 4

000

form

, et

hano

l,

ethy

l ac

etat

e,

dich

loro

met

hane

, he

xane

, m

etha

nol,

pe

ntan

e an

d te

tra-

hydr

ofur

an

Alu

min

ium

oxi

de

6-15

ro

und

or

3-12

ac

id/

Aqu

eous

and

non

-60

A

irre

gula

r ba

se

aque

ous

solv

ents

2.

Rev

erse

d-Si

lica

gel

with

ph

ase

buty

l(C

4),

eth

yl(C

2),

Ace

tic a

cid,

he

xyl(

C6)

, oc

tyl(

Cs)

, ac

eton

e,

octa

decy

l(C

,s)'

amin

o ac

eton

itri

le,

(NH

2)' c

yano

or

nitr

ile

buff

er s

olut

ions

(C

N),

diol

(O

H),

et

hano

l, f

orm

ic

met

hyl(

CH

3),

dim

ethy

l ro

und

or

3,4,

5,7

2-9

acid

, m

etha

nol,

am

ino

(N(C

H3)2

),tr

imet

hyl(

CH

3h,

irre

gula

r 10

and

30-

50

2-14

te

trah

ydro

fura

n n

itro

(N0

2),

phen

yl

30-5

0 an

d w

ater

(C

6HS)

' dip

heny

l (C

6HS

b su

lfon

ic

acid

(SA

), qu

arte

rnar

y am

mon

ium

(SB

), do

decy

l, do

cosy

l an

d ce

llulo

se(a

cety

l)

M. K. Ghosh, HPLC Methods on Drug Analysis© Springer-Verlag Berlin Heidelberg 1992

3.

Ion-

exch

ange

Si

lica

gel

60 A

C

hrom

atog

raph

y A

nion

(ba

se),

roun

d o

r 2.

5,3.

5 1-

13

HC

l, C

H3C

OO

H.

1000

A

irre

gula

r ca

tion

(ac

id)

irre

gula

r 10

,5-1

2,20

1.

5-7.

5 bo

ric

acid

buf

fer,

(a

min

opro

pyl,

1-

9 tr

is H

CI-

buff

er,

tetr

aalk

ylam

mon

ium

, \.

5-7

0.5

M N

aCI

acet

ate

carb

oxyl

ic a

cid

or

7-10

bu

ffer

and

NaO

H

sulf

onic

aci

d gr

oups

so

luti

on

4.

Ion-

pair

R

ever

sed-

phas

e sy

stem

s ro

und

or

3-10

1-

9 A

ceto

nitr

ile,

buf

fer

Chr

omat

ogra

phy

mod

ifie

d by

ion

-pai

r ir

regu

lar

solu

tion

s, m

etha

nol

and

reag

ents

to

anio

nic

wat

er w

ith

mod

ifie

rs,

or

mod

ifie

rs

5.

Par

titi

on

Liq

uids

; li

quid

pro

pane

, A

ceto

nitr

ile,

C

hrom

atog

raph

y di

met

hyl

sulf

oxid

e,

acet

one,

chi

oro

-ni

trom

etha

ne a

nd

form

, di

oxan

e,

CC

I 4,

coat

ed o

n in

ert

isoo

ctan

e, h

exan

e,

soli

d as

sili

ca a

nd

met

hano

l/w

ater

bo

nded

wit

h va

riou

s an

d pe

ntan

e fu

ncti

onal

gro

ups

(am

ino,

cy

ano,

dio

l an

d ni

tro)

6.

Gel

chro

mat

o-Si

lica

wit

h 50

-4.1

08

3-17

C

hlor

ofor

m,

tetr

a-gr

aphy

gl

ycer

opro

pyl,

ethe

r hy

drof

uran

, bu

ffer

an

d st

yren

e-di

viny

l, so

luti

ons

and

poly

styr

ene,

pol

yacr

ylam

ide,

w

ater

m

etha

cryl

ate,

gly

ceri

n,

co-p

olym

er w

ith f

unct

iona

l gr

oups

(am

ino

prop

yl,

tetr

a alk

yl a

mm

oniu

m

or

sulf

onic

aci

d

Tab

le 5

. (C

onti

nuat

ion)

Nam

e C

hem

ical

nat

ure/

fu

ncti

onal

gro

ups

7.

Siz

e-E

xclu

sion

G

el-P

erm

eati

on

Chr

omat

ogra

phy

Chr

omat

ogra

phy

(GP

C)

(non

aqu

eous

)

Gel

-Fil

trat

ion

Chr

omat

ogra

phy

(GF

C)

(aqu

eous

)

8.

Aff

inity

P

olym

er b

ased

C

hrom

atog

raph

y

9.

Mis

cell

aneo

us

a)

Fo

r B

iopo

lym

er

Silic

a ge

l w

ith

anio

n-ex

chan

ge,

silic

a ge

l w

ith

cati

on-e

xcha

nge,

si

lica

gel

wit

h di

ffer

ent

reve

rsed

­ph

ases

Por

e-si

ze

80--

> 2

000

A

1000

A

10,3

0

33

Par

ticl

e sh

ape

Par

ticl

e si

ze, ~m

9-20

6-30

10--

15

5,8,

10

pH-r

ange

/ S

olve

nts

as

reac

tion

el

uent

acid

/ ba

sel

neut

ral

2-7

Ace

tone

, di

met

hylf

orm

amid

e,

diox

ane,

met

hyl

ethy

l ke

tone

, an

d te

trah

ydro

fura

n

Buf

fer

solu

tion

s,

salt

sol

utio

ns

and

wat

er

Ace

toni

tril

e,

buff

er s

olut

ions

, sa

lt s

olut

ions

, tr

is-b

uffe

r an

d w

ater

Ace

toni

tril

e,

buff

er s

olut

ions

, sa

lt s

olut

ions

, tr

is-b

uffe

rs a

nd

w

ater

b)

Fo

r S

ugar

s A

min

o o

r oc

tade

cyl

5,8,

10

80%

Ace

toni

tril

e ph

ase

on s

ilica

gel

, an

d w

ater

, so

dium

ca

tion

-exc

hang

e on

ph

osph

ate

and

styr

ene-

divi

nyl

sodi

um a

ceta

te

benz

ene,

so

luti

ons

cati

on-e

xcha

nge

on

styr

ene-

divi

nyl

benz

ene

and

or C

a-fo

rm o

r P

b-fo

rm

c)

Fo

r am

ino

Silic

a ge

l w

ith

amin

o,

5,9,

10

Ace

toni

tril

e,

acid

s an

d oc

tyl

and

nitr

ile

buff

er. s

olut

ions

, nu

cleo

tide

s ph

ases

, 0.

1 %

tri

fluo

ro

cati

on-e

xcha

nge

on

5,10

ac

etic

aci

d, 0

.1 M

st

yren

e-di

viny

l be

nzen

e,

trie

thyl

am

ine

cati

on-e

xcha

nge,

5,

10

and

wat

er

H-f

orm

, pr

olin

e o

r va

line

-cop

per-

com

plex

, am

ino-

phas

e w

ith

ioni

c am

ino

acid

s or

alb

umin

d)

Fo

r en

anti

omer

s C

hira

l ph

ase-

5 n-

Hex

ane,

dio

xane

, po

lyac

ryla

mid

e n-

prop

anol

, te

tra-

on s

ilica

gel

hy

drof

uran

and

var

ious

ot

her

orga

nic

solv

ents

Cel

lulo

se a

ceta

te

10,1

5-24

E

than

ol,

25-4

0 m

etha

nol,

80

% n

-hex

ane,

n-

prop

anol

, w

ater

an

d va

riou

s ot

her

orga

nic

solv

ents