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An update on research progress. Human papillomavirus and breast cancer. Giles Davies. Breast Cancer. Most prevalent type of Cancer amongst women Second leading cause of cancer related deaths Multiple risk factors associated with Breast Cancer No specific cause for up to 80% of the cases - PowerPoint PPT Presentation
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HUMAN PAPILLOMAVIRUS AND BREAST CANCER
Giles Davies
An update on research progress
BREAST CANCER Most prevalent type of Cancer amongst
women Second leading cause of cancer related
deaths Multiple risk factors associated with Breast
Cancer No specific cause for up to 80% of the cases Incidents rising, urgent prevention or cure
needed
HUMAN PAPILLOMAVIRUS (HPV) HPV is from the family of papillomaviruses
Discovered by Prof Zur-Hausen in genital warts
in 1976
High Risk HPV infection is accountable for over
90% of Cervical cancer cases
HPV also associated with other cancers such
as genital, skin, lung, oral cancers and more
recently with Cardiovascular disease!
HPV Grouped according to their physiological and
biological similarities A1 – A15 (A5, A6, A7, A9 & A11 are carcinogenic) Species A7 includes HR HPV types 18, 56, 66,
39, 45, 59, 68 and 70 Species A9 includes HR HPV types 16, 31, 33,
35, 52, 58, and 67 Further divided into high risk (HR) and low risk
(LR) HPV types HR HPV are carcinogenic – malignant lesions LR HPV associated with warts - benign lesions. Vaccines for HPV types 16, 18 (11, 6)
HPV IN BREAST TISSUE - OUR STUDY Aim: to determine the presence of HPV in normal,
benign and malignant breast tissue HPV discovered in Breast tissue from other research-
controversial results Most research carried out on paraffin embedded
samples – poor quality DNA Most research studies focused only on HPV types 16
and 18 No HPV in Breast Cancer research from UK
SAMPLES COLLECTED AND PROCESSED SO FAR 50 fresh tissue samples collected from
Kingston Hospital Fresh tissue protected in Allprotect
reagent DNA, RNA and Protein extracted from 30
samples - disruption & homogenization easier using TissueLyser
PCR and Gel Electrophoresis carried out on 30 samples
AmpliSens HPV HCR genotype-Eph PCR kit used, targets HPV 16, 31, 33, 35, 18, 39, 45, 59, 52, 56, 58, 88
HPV TYPES DETECTED IN SAMPLESSample HPV 16 HPV 31 HPV 33 HPV35 HPV 18 HPV 39 HPV45 HPV 59
50 51 5253 545556 5758 596061 62 63 64 6566 6971 72 73 75 76
GEL ELECTROPHORESIS IMAGES
340bp
475 bp395 bp
723 bp
GEL ELECTROPHORESIS IMAGES
340bp425 bp
395 bp
723 bp
PREVALENCE OF HPV TYPES
HPV 16 = 0
HPV 31 = 20%
HPV 33 = 0
HPV35 = 20%
HPV 18 = 26%
HPV 39 = 46%
HPV45 = 40%
HPV 59 = 46%
So far 62% HPV detected in 24 patients
CONCLUSIONS So far HPV discovered in a high number of patients 86% of patients with HPV have multiple infections Most prevalent HR HPV types 39, 59 and 45 which are similar to
HPV 18 Very low prevalence of vaccine HR HPV types (18 and 16)
FUTURE WORK More samples required Why HPV present in breast tissue? How HPV entered the breast tissue? Is HPV associated with Breast Cancer? Presence of HPV in protein