Upload
others
View
9
Download
0
Embed Size (px)
Citation preview
Tissue Engineering Lab/ Faculty of Medicine
Tissue & organ printing: the future in tissue engineering ?
Hybrid bioprintingBiofabrication of complex or vascularized tissue
Hybrid bioprinting
Self-assembling spheroids
Pre-processing
BiofabricationPost-
processing
• 3D in vitro models
• Organ-on-a-chip
• Regenerative medicine
Patient-specific biomimetic tissue analogues
PhDs Mendy Minne – Prof. Heidi Declercq
Contact: [email protected]
✓ Disease modeling
✓ Personalized medicine
✓ Drug screening
Maturation phase I Maturation phase II
Microtissues Smart biomaterials External stimuli
References: Mironov V. et al. Biomaterials 2009; De Moor L. et al. Biofabrication 2018; De Moor L. et al. Annals of
Biomedical Engineering 2019; Vercruysse C. et al. In preparation; Roosens A. et al. Journal of Tissue Engineering
and Regenerative Medicine 2019; Colle J. et al. Journal of Materials Science-Materials in Medicine 2020
Microtissues
• Non-adhesive agarose microwells
• High-throughput
• Pores 200-400 µm (2865-1585/microwell)
• Uniform & printable size
Microtissues
Spheroid generation
Tissue specific spheroids
Cartilage FibrocartilageBoneValvular tissue Adipose tissue
• Engineering of 3D living structures
• Precise placing of different cells, extracellular
matrix and biomolecules in 3D
• Layer-by-layer additive manufacturing
Bioprinting
Smart biomaterials & external stimuli
Ink
• Bioinks with electroactive components
• Tissue fusion permissive hydrogels
• Patient-derived bioinks
• 3D aligned guiding systems
Cell modalities
Bioink
Tissues:
• Repeating functional units
• Self-organizing systems
Vascularized spheroids
Lineage-specific differentiation of stem & primary cells
Self-assembling of endothelial & supporting cells
✓ Multi- and heterocellular spheroids
✓ Increasing complexity
✓ 3D mimick of tissue
PDMS mold PDMS mold agarose microwell
liquid agarose