8/17/2014 First test microscope PZO, DIC Pluta

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Invisible Life " INVISIBLE LIFE. FORUM COM » INSTRUMENTAL and DIY Forum » First test microscope PZO, DIC Pluta

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First test microscope PZO, DIC

Pluta« on: July 31, 2013, 20:03:43 pm »

francisco

Hero

Posts: 752

Reputation 5Hi

I purchased a PZO microscope equipped with DIC Pluta.

's first impression is quite good, it is a sturdy and good mechanical precision

microscope.

A level on Dec optical system seems interesting and a value very favorable. I

guess the most modern systems in December; Olympus, Nikon, Zeiss ....

proporcinaran more accurate results that this system PZO.

microscope came without me lighting system which, provisionally, I improvised

a pvc pipe with 1W Led waiting to find some enlightening for adaptation.

The binocular head is apparently very few trinocular heads that were

manufactured for these microscopes so to make the video have replaced the

head by the cctv camera. It is a heavy and unpleasant task looking specimen

through a field of view as small as that provided by the camera.

The head diameter is 42mm I think the same as the Olympus BH Is it okay ?.

Buscare some head to adapt and have vision and puero to camera. Do you

know of any trinocular head (besides the Olympus) having this measure ?.

DIC My experience is nil so any suggestions, criticism, help and information will

be received with great interest and appreciation.

What is the difference between the different prisms 10X, 20X, 40X

(condenser)?

Thank

Francisco http://youtu.be/16CuJYcNhdA

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Re: First test PZO microscope, DIC RogelioMoreno

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8/17/2014 First test microscope PZO, DIC Pluta

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Pluta« Reply # 1 on: July 31, 2013,

23:27:06 pm »

Hero

Posts: 928

Reputation 2

Sex: Francisco, Very interesting, congratulations on your new DEC computer. With

practice you'll master the best settings to get the results you want. Prisms

capacitor (10x, 20x, 40x, etc) are to be used for objectives with the same

increases as specified in the prism, ie one objective should be used with 10x

10x objective and so on. , you're right in terms of diameter heads and Olympus

BH BH2 are 42mm. I'm not sure but I think Nikon Optiphot / 43mm or 42mm is

Labophot. have to ask a seller on ebay. Regarding configuration DIC: if you

use lenses designed for that microscope then I do not think you need to move

the prism goes up goals (I have understood that the Dec PZO Fits any such

objectives and allows the prism goes above objectives can move up / down to

locate the prism in the back focal plane of the objective (to get the best DIC)).

I would see pictures of the DIC system espcificamente area of the prism is

above objectives. Greetings, Rogelio

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Re: First test PZO microscope, DIC

Pluta« Reply # 2 on: July 31, 2013, 23:29:45 pm

»

Hugo

Hero

Posts: 815

Reputation 17

Sex:

Hi Francisco. I really like this goes DIC, very good resolution. The polarizer

makes the funds are colored ("tint plate" or quarter-lambda if I remember

correctly) is fixed or can be removed?

A priori, without taking action, could be a Lomo or Carl Zeiss Jena head works

for you, East microscopes were quite compatible Were you thinking of a head

BH2?

Zeiss West 43,5mm measures

42mm Olympus BH2 Regards Hugo

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 3 on: July 31, 2013, 23:37:33 pm

»

Hugo

Hero

From this comes the name ... Maksymilian Pluta

http://micro.magnet.fsu.edu/optics/timeline/people/pluta.html Hugo

8/17/2014 First test microscope PZO, DIC Pluta

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Posts: 815

Reputation 17

Sex:

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Re: First test PZO microscope, DIC

Pluta« Reply # 4 on: July 31, 2013, 23:48:02 pm

»

Jose Ricardo

User Jr

Posts: 67

Reputation 0 . Hola Francisco

Congratulations on your purchase. I do not know the quality of the most

modern in December and I have no possibility to compare. What if I think is

that the optics have come a long way and the level of the current goals is very

high. Just look at the "Chinese" objectives that offer very nearby images to

the middle range of Olympus and Nikon a few years ago, for a fraction of the

cost. Regarding the quality of this December, I can say I've seen pictures in

forums that used hybrids thereof and the result is impressive. equipment is

easy to use (at least if you limit yourself to December, which is what I have).

The imaging requires lots of light, 1W not have much. You can easily contruirte

an aluminum cylinder with a powerful LED on the end (no information on this.)

I have a box of 100w halogen lamps and the 40x objective notice that I need

light. Regarding the head, I use a trinocular the BH2. It is a little loose and

therefore misaligned with the optical axis (although this is fixable with a bit of

DIY). Finally, with respect to the difference of the prisms, do not quite

understand what you're asking. But you commented elocubración a mine on

them: because there prisms for each of the increases (causing diffractions on

demand for each of them) and one common to reassemble the scattered light,

I understand that the result of the prism assembly homologous target and the

image size / diffraction corrected for the common prism. I'll be happy if I can

answer to the extent of my limited knowledge, to some concerns over this

element. Glad there is another forero with this team and to share knowledge

and experience. Greetings. R. Jose

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Re: First test PZO microscope, DIC

Pluta Hugo

8/17/2014 First test microscope PZO, DIC Pluta

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« Reply # 5 on: August 1, 2013, 00:01:02

am »

Hero

Posts: 815

Reputation 17

Sex:

If the head is loose then BH2 should be tailored to the Lomo Zeiss and they

are bigger 1.5mm. And better too because there are many of these. Hugo

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 6 on: August 1, 2013, 00:22:45

am »

francisco

Hero

Posts: 752

Reputation 5 Quote: Ricardo Jose on July 31, 2013, 23:48:02 pm

. Hola Francisco

Congratulations on your purchase. I do not know the quality of the most modern in

December and I have no possibility to compare. What if I think is that the optics

have come a long way and the level of the current goals is very high. Just look at

the "Chinese" objectives that offer very nearby images to the middle range of

Olympus and Nikon a few years ago, for a fraction of the cost. Regarding the quality

of this December, I can say I've seen pictures in forums that used hybrids thereof

and the result is impressive. equipment is easy to use (at least if you limit yourself

to December, which is what I have). The imaging requires lots of light, 1W not have

much. You can easily contruirte an aluminum cylinder with a powerful LED on the

end (no information on this.) I have a box of 100w halogen lamps and the 40x

objective notice that I need light. Regarding the head, I use a trinocular the BH2. It

is a little loose and therefore misaligned with the optical axis (although this is

fixable with a bit of DIY). Finally, with respect to the difference of the prisms, do not

quite understand what you're asking. But you commented elocubración a mine on

them: because there prisms for each of the increases (causing diffractions on

demand for each of them) and one common to reassemble the scattered light, I

understand that the result of the prism assembly homologous target and the image

size / diffraction corrected for the common prism. I'll be happy if I can answer to

the extent of my limited knowledge, to some concerns over this element. Glad

there is another forero with this team and to share knowledge and experience.

Greetings. R. Jose

Jose Ricardo Hi

Thanks for the reply and offer. Your knowledge and experience in this system

will be of great help.

Yeah, 1W is poor lighting. I have received a specific illuminator for these

microscopes, in this case the PZO Studar tomorrow for themselves to adapt. I

hope I have the problem that the field of view of the eyepiece is not full

8/17/2014 First test microscope PZO, DIC Pluta

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disappear even think this will be because the previous owner has tampered

with the internal lenses of the lighting system.

Personally despite the targets used in video "standard "(The 20X is a phase

contrast Nikon because I do not have another 20X) the outcome seemed

pretty interesting.

Greetings

Francisco

In line

Re: First test PZO microscope, DIC

Pluta

« Reply # 7 on: August 1, 2013, 00:37:29

am »

francisco

Hero

Posts: 752

Reputation 5 Quote: RogelioMoreno on July 31, 2013, 23:27:06 pm

Francisco, Very interesting, congratulations on your new DEC computer. With practice

you'll master the best settings to get the results you want. Prisms capacitor (10x,

20x, 40x, etc) are to be used for objectives with the same increases as specified in

the prism, ie one objective should be used with 10x 10x objective and so on. ,

you're right in terms of diameter heads and Olympus BH BH2 are 42mm. I'm not

sure but I think Nikon Optiphot / 43mm or 42mm is Labophot. have to ask a seller

on ebay. Regarding configuration DIC: if you use lenses designed for that

microscope then I do not think you need to move the prism goes up goals (I have

understood that the Dec PZO Fits any such objectives and allows the prism goes

above objectives can move up / down to locate the prism in the back focal plane of

the objective (to get the best DIC)). I would see pictures of the DIC system

espcificamente area of the prism is above objectives. Greetings, Rogelio

Hi Rogelio

Regarding the prisms was asking because apparently any combination of

condenser and objective prism gives me the same effect, ie ejmplo; condenser

prism 10X or 10X objective and 20X or 40X offer me the same effect, same

with condenser prism 20X or 40X and 10X objective ..... I repeat it yet

apparently not well know the DIC sitemas.

I think This system is used for both DIC (position 1 of the "head") and also

interference microscope (positions 2 and 3 of "head"). The particular aims of

this system are used for the band system for determining the refractive index

,. thickness of the sample ... etc but they are not valid for "normal" image for

only double or so separate images are observed.

Tomorrow make a few photographs for publication system

Regards

Francisco

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 8 on: August 1, 2013, 00:50:17

am »

francisco

Hero

Posts: 752

8/17/2014 First test microscope PZO, DIC Pluta

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Reputation 5

Quote: Hugo on July 31, 2013, 23:29:45 pm

Hi Francisco. I really like this goes DIC, very good resolution. The polarizer makes

the funds are colored ("tint plate" or quarter-lambda if I remember correctly) is

fixed or can be removed?

A priori, without taking action, could be a Lomo or Carl Zeiss Jena head works for

you, East microscopes were quite compatible Were you thinking of a head BH2?

Zeiss West 43,5mm measures

42mm Olympus BH2 Regards Hugo

Hi Hugo

Thanks for your comment and information

I think is a very attractive system for performance and price

The system uses two polarizers, the head which is movable and condenser

which is movable and removable since it also is used in the condenser variable

phase contrast PZO (I think unique to this brand) that allows a single capacitor

with the same goals and contrast both positive and negative phases. The

system is based on darken polarizer by rotating either of the two rings has the

PHZ phase goals. Simple and very interesting system.

tried the Zeiss photomicroscope my head and fits well but this kind of heads

are not in trinocular version as the port for the camera is built into the

Optovar. I think Lomo only has a trinocular head and the few that have come

out on Ebay lozacilazo beloved, I guess because of its rarity.

's why I'm looking for some Po trinocular head approx. 42mm, Olympus or

other mark

Regards

Francisco

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 9 on: August 1, 2013, 1:05:39 am

»

Hugo

Hero

Posts: 815

Reputation 17

Sex:

Zeiss has used these Trinocular with looking on Ebay "Zeiss trinocular" out

quite a bit. Hugo

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 10 on: August 1, 2013, 9:52:53

am »

francisco

Hero

Posts: 752

Reputation 5 Thanks Hugo for the information

Regards

Francisco

In line

8/17/2014 First test microscope PZO, DIC Pluta

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Re: First test PZO microscope, DIC

Pluta« Reply # 11 on: August 1, 2013, 9:57:33

am »

ABEL

Technical Staff

Manager

Hero

Posts: 1774

Reputation 11

Congratulations on the DIC. The PZO not know you could have a DIC. It's an

awesome microscope.

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 12 on: August 1, 2013, 10:54:31

am »

Jose Ricardo

User Jr

Posts: 67

Reputation 0 . Hola Francisco ; Regarding what you state that "any combination of

condenser and objective prism gives me the same effect" this should not be.

The combination of prism and lens is the featuring more sharpness in the

image and is clearly the best. If it is true that the effect of December with the

other combinations are seen, but the image is significantly worse. You must

make sure that the polarizing lenses are effectively cross. With the analyzer

no problem because it takes your system fixing (if you have it in position

crossed 45 degrees). In the case of the capacitor; This position does not have

a fixed point is mounted to "eye." What I do is align the screw of the capacitor

with the reference mark of the polarizer. And I adjust the polarizer on the "X"

position. So I make sure that they are indeed crossed. acquired on Dec. When

the polarizer had no condenser, replaced it with a polarized lens I had. The

results were somewhat variable from one session to another, depending on

the position of the polarizer that day. Greetings.

In line

Re: First test PZO microscope, DIC

Pluta« Reply # 13 on: August 1, 2013, 13:01:04

pm »

francisco

Hero

Posts: 752

Reputation 5 Thanks Abel.

DIC is an old I think dates from approx. 1960 - 1970

Jose Ricardo Thanks for the info, I'm still not familiar with this system and not

doing something correctly. I appreciate your advice and information

I was fortunate to acquire joint DIC in very good condition, I think it was new

for any sign of use is observed.

arrive with the polarizer fitro condenser and this is marked, like that of "head

"with an X to indicate when they are crossed, in fact no sample in the field of

view and the two filters in the x position the image is completely black.

microscope if you had more than evident signs of having been treated with

little " dedication "

Some photographs System

8/17/2014 First test microscope PZO, DIC Pluta

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DSC00094.JPG (59.56 kB, 640x480 - viewed 220 times.) DSC00095.JPG (63.71 kB,

640x480 - viewed 218 times.) DSC00108.JPG (50.67 kB, 640x480 - viewed 207

times.) DSC00105.JPG (54.12 kB, 640x480 - seen 219 times).

8/17/2014 First test microscope PZO, DIC Pluta

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8/17/2014 First test microscope PZO, DIC Pluta

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Re: First test PZO microscope, DIC

Pluta« Reply # 14 on: August 1, 2013, 13:56:58

pm »

Jose Ricardo

User Jr

Posts: 67

Reputation 0 If indeed it is the right place if you see the dark field. What I wanted to say is

that the capacitor has no fixed position mounting and must be aligned by eye.

If it is not graduation polarizer is incorrect. Dec. My buy parts. Specifically three

Ebay sellers: head, condenser and condenser polarizer. The entire team

seemed too what they asked for. microscope also was / is in a rather poor

state. Although I have removed almost everything to clean and grease, dust

still has in the next lens diaphragm Illuminator (this cleanup is pending). With

the 10x objective this powder as well as deficiencies in lighting (gradient color

temperature of the lamp filament is seen) is appreciated. The latter hope to

correct going Led lighting and placing a diffuser directly in front (like the BH2

and the like). Salu2.

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