IDRaman Mini 2.0 Operating Instructions - oceanoptics.com · IDR-MINI-2-KIT – For your convenience, the package of 144 2 mL sample vials is combined with a point-and-shoot solid

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IDRaman mini 2.0 Installation and Operation Manual

For Products: IDR-MINI-2, IDR-MINI-2-KIT, IDR-MINI-VIAL, IDR-MINI-SERS-ADAPTER Document: 000-40000-011-02-201601

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www.oceanoptics.com

Copyright © 2015 Ocean Optics, Inc. All rights reserved.

Trademarks All trademarks within are the property of their respective owners.

Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible, but no warranty or fitness is implied. The information

provided is on an “as is” basis. Ocean Optics, Inc. shall have neither liability nor responsibility to any person or entity with respect to any loss or

damages arising from the information contained in this manual.

Ocean Optics, Inc. 830 Douglas Ave. Dunedin, FL 34698 USA Manufacturing & Logistics 4301 Metric Dr. Winter Park, FL 32792 USA

Ocean Optics Asia 666 Gubei Road Kirin Tower Suite 601B Changning District Shanghai PRC, 200336

Sales & Support Geograaf 24 6921 EW Duiven The Netherlands Manufacturing & Logistics Maybachstrasse 11 73760 Ostfildern Germany

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Important Safety Notices

The laser described here is safe to operate, provided the user pays attention to all safety

warnings:

1. Post warnings in the area of the laser beam to alert those present.

2. Keep all unauthorized personnel out of the area where the laser is operated.

3. Whenever the laser is running and the beam is not in use, it is good operating practice to mechanically block the path.

4. Never look directly into the laser source or scattering laser light from any reflective surface. Never sight down the beam into the source.

5. Maintain experimental setup at low heights to prevent inadvertent beam-eye contact.

6. As a precaution against accidental exposures to the output beam or its reflection, operators should wear laser safety glasses attenuated to the wavelength being generated.

For additional information:

Laser Institute of America 13501 Ingenuity Drive, Suite 128 Orlando, FL 32826

Toll Free: 1-800-345-2737 Tel: 1-407-380-1553 Fax: 1-407-380-5588


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Laser Safety

WARNING

This device is intended for use by trained personnel only. Use of controls or adjustments, or performance of procedures other than those described in this manual may result in hazardous laser radiation exposure.

This is a class 3B laser device and up to 100 mW of 785nm (NIR) laser light may be emitted during data acquisition. Only personnel trained in laser safety protocols should be allowed to use this device.

The aperture warning label indicates where laser radiation may be emitted from the device when using the point-and-shoot attachment. The laser safety goggles provided must be worn when using the point-and-shoot attachment.

Internal maintenance or repair must be performed at the factory or by authorized personnel only.


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Locations of the Warning Labels

Remote Interlock

The IDRaman mini 2.0 has remote interlock capability via the ID pin in the Micro USB port. Contact Ocean Optics for more information on the remote interlock connector.

Nominal Optical Hazard Distance Calculations

The IDRaman mini 2.0 is a Class 3B laser device, containing a 785nm, 100mW rated laser diode. These Class 3B devices are hazardous when intra-beam ocular (eye) exposure occurs, i.e., when one intentionally or unintentionally looks directly into the beam from within the Nominal Optical Hazard Distance (NOHD). Never look directly into any laser beam. The information and calculations below contain details of the intra-beam NOHD calculation for the IDRaman mini 2.0 (785) in accordance with the recognized international standard, IEC-60825-1, Safety of Laser Products, 2nd ed., 2007.

Assumptions

Safety interlock(s) failed or bypassed; Raster (motor) stalled or stopped; Laser on continuously at maximum possible component power of 140mW, CW.

General Equation

N. O. H. D. =√

4 ∗ 𝑃𝜋 ∗ 𝑀. 𝑃. 𝐸.

− 𝑎

∅


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Condition 1

Sample door open; Safety interlock defeated or failed; No auxiliary lenses present:

N.O.H.D. = 1.32 m (4 feet 4 in.)

Condition 2

Point and Shoot attachment installed:

N.O.H.D. = 2.64 m (8 feet 8 in.)

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Table of Contents

About This Manual ......................................................................................................... iii Document Purpose and Intended Audience .............................................................................. iii Document Summary .................................................................................................................. iii Product-Related Documentation ............................................................................................... iii

Upgrades ....................................................................................................................... iv

Warranty ........................................................................................................................ iv

Chapter 1: Introduction ..................................................................... 1

Overview ....................................................................................................................... 1

Features ........................................................................................................................ 1

Models ........................................................................................................................... 2

About Raster Orbital Scanning (ROS) ........................................................................... 2

What’s In the Box .......................................................................................................... 3 IDR-MINI-2-KIT .......................................................................................................................... 3 Accessories ............................................................................................................................... 4

Point and Shoot Adapter ................................................................................................ 4

SERS Adapter ............................................................................................................... 6

Chapter 2: Standalone Operation ..................................................... 7

Overview ....................................................................................................................... 7

Controls and Indicators .................................................................................................. 7 Acquiring a Spectrum ................................................................................................................ 11 Saving a Spectrum .................................................................................................................... 12 Managing Libraries .................................................................................................................... 13 Calibrating the Device ............................................................................................................... 13 Autosaving ................................................................................................................................. 15 Scan Delay ................................................................................................................................ 16 Setting the Laser Power ............................................................................................................ 17 Setting the Laser Raster ............................................................................................................ 18 Setting Sleep Delay Time .......................................................................................................... 19 Changing the PIN ...................................................................................................................... 20

Table of Contents

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Chapter 3: Operation with OceanView ............................................. 23

IDRaman mini 2.0 Start-up with OceanView Software ................................................... 23 Start-up with Windows 7, 8 and 10 ............................................................................................ 23

Operating with OceanView Software ............................................................................. 24 Acquiring a Spectrum ................................................................................................................ 26 Saving Data ............................................................................................................................... 28 Device Libraries ......................................................................................................................... 28

Appendix A: Specifications ............................................................... 37

Index ................................................................................................... 39

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About This Manual

Document Purpose and Intended Audience

This document provides you with information to get your IDRaman mini 2.0 set up and operating.

What’s New in this Document

This version of the IDRaman mini 2.0 Installation and Operation Manual updates the hardware and software interface information for version 2.0, including the libraries offered.

Document Summary

Chapter Description

Chapter 1: Introduction Contains a list of product features and the model types available, and offers an explanation of ROS.

Chapter 2: Standalone Operation Provides instructions for setting up and operating the IDRaman mini 2.0 in standalone mode

Chapter 3: Operation with OceanView Provides instructions for using the IDRaman mini 2.0 when connected to a computer using OceanView software.

Appendix A: Specifications Provides a list of product specifications.

Product-Related Documentation

You can access documentation for Ocean Optics products by visiting our website at http://www.oceanoptics.com. Select Support → Technical Documents, then choose the

appropriate document from the available drop-down lists.

Detailed instructions for OceanView Spectrometer Operating Software are located at: http://oceanoptics.com///wp-content/uploads/OceanViewIO.pdf

A list of the available Raman libraries is located at: http://oceanoptics.com/product/idraman-mini-software-libraries/

http://www.oceanoptics.com/

http://oceanoptics.com/product/idraman-mini-software-libraries/

About This Manual

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Upgrades Occasionally, you may find that you need Ocean Optics to make a change or an upgrade to your system. To facilitate these changes, you must first contact Customer Support and obtain a Return Merchandise Authorization (RMA) number. Please contact Ocean Optics for specific instructions when returning a product.

Warranty Our 1-Year Warranty covers Ocean Optics IDRaman products – regardless of the application – from manufacturing defects. The warranty covers parts and labor needed to repair manufacturing defects that occur during the warranty period. We also will cover the costs of shipping warranty-related repairs from our customers to Ocean Optics and from us to our customers.

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Chapter 1

Introduction

Overview The IDRaman mini 2.0 is the next generation of the IDRaman mini Handheld Raman System for Raman analysis ranging from chemical and explosive agent detection in the field to quality assurance and quality control sampling routines in the factory. The 2.0 version includes a preloaded, editable library of about 30 Raman spectra. Additional libraries of up to 9,000 spectra are also available for purchase. An adapter kit is also available for measuring SERS. Ask your Ocean Optics representative for more details.

The ID Raman mini 2.0 is the smallest, most powerful handheld Raman device available today. Designed to make rapid and accurate measurements that identify, authenticate and verify samples, the IDRaman mini 2.0 is ideal for:

Authentication analysis

Counterfeit detection

Rapid material identification

Verification of incoming materials

Harsh and demanding environments

In-line or at-line testing

From rugged field measurements of chemical and explosive agents to quality assurance and quality control in the laboratory, the IDRaman mini 2.0 is a truly compact choice for fast and accurate measurements.

IDRaman mini 2.0 includes an easy to use calibration routine to ensure the most accurate Raman shift data is acquired. Measuring most samples in less than 9 seconds, the IDRaman provides visual confirmation of results and displays both the sample and library spectrum with a confidence factor. You can store a library of thousands of compounds in the IDRaman. Using a proprietary fluorescence rejection algorithm, the IDRaman mini 2.0 minimizes both false positive and false negative results. With IDRaman’s Raster Orbital Scanning technology, the limitation of weak signals from unknown and non-uniform samples is overcome.

Features

Long battery life - >11 hours from 2 Lithium AA batteries

Light weight – 330 grams (11.75 oz.)

Compact size - 9.4 x 7.4 x 3.8 cm (3.7 x 2.9 x 1.5 in)

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Great flexibility – measures both liquids (vials) and solid samples (with accessory kit)

Rugged design – made from solid aluminum (IP67-rated housing)

High visibility display – 7.4 cm (2.9”) resistive touch display

Easy to use – intuitive user interface via touch screen

High speed acquisition – visual confirmation in <9.0 seconds

Self-calibration – includes onboard software driven calibration

Raster Orbital Scanning (ROS) function -- allows detection of inhomogeneous samples without sample degradation

Library recognition - use of proprietary data analysis algorithm for high accuracy identification without low false positives and false negatives

Models The following products are available:

IDR-MINI-VIAL – A package of 144 (2 mL) sample vials

IDR-MINI-2-KIT – For your convenience, the package of 144 2 mL sample vials is combined with a point-and-shoot solid sampling attachment plus laser safety glasses in a handy storage case.

IDR-MINI-SERS-ADAPTER – A sampling accessory for SERS

About Raster Orbital Scanning (ROS) The Ocean Optics IDRaman mini 2.0 uses ROS to rapidly scan a tightly focused beam in an orbital pattern, allowing lower average power to produce high-integrity data from a larger area of the sample without sample ignition or damage.

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A tightly focused beam may result in noisy signals or missing the Raman active target completely, leading to false negative readings from unidentified samples.

Increasing the spot size of the laser dilutes the valuable information about the material, leading to inconclusive matches or false identification of samples.

ROS increases the effective size of a tightly focused beam. Information is obtained with complete integrity while sampling the large area needed for complex mixtures or irregularly shaped samples, leading to confident identification.

What’s In the Box

IDR-MINI-2-KIT

IDRaman mini Device

The IDRaman mini 2.0 device uses two AA batteries.

Box of 144 2 mL Round Vials

Use these empty vials to collect your samples.

One Calibration Standard

Use this standard vial to calibrate your instrument.

Laser Safety Goggles

Wear the goggles while operating the laser.

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Micro-USB Cable

Use this cable to connect the IDRaman mini 2.0 to a computer for power (USB Type A to Micro-B).

Pelican Case

Use the case to store the instrument and vials.

Accessories

IDR-MINI-VIAL

These are 144 additional 2 mL vials for collecting your own samples.

Ocean Optics offers the following additional products to work with your IDRaman mini 2.0 (available at an extra cost). Visit us at www.oceanoptics.com for a complete list of products available for all of your spectroscopy needs.

OceanView Software

OceanView is the latest generation of operating software for Ocean Optics devices. It is a Java-based spectroscopy software platform that operates on Windows, Macintosh and Linux operating systems. This software can be downloaded from Ocean Optics is used to control your IDRaman mini 2.0 device and provides additional functionality for managing the sample libraries.

SERS Adapter

The SERS Adapter (IDR-MINI-SERS-ADAPTER) is available for Surface Enhanced Raman Spectroscopy (SERS) where analytes (your sample) are absorbed onto a noble metal (in this case either Au or Ag nanoparticles) surface prior to analysis in order to potentially enhance the Raman signal. SERS enables ppb and even ppt-level detection of chemical and biological materials quickly and easily in the field. It also has many applications for pharmaceuticals, explosives, and tags for anti-counterfeiting.

The SERS Adapter attaches to the Point and Shoot (PS) Adapter (see SERS Adapter).

SERS Substrates

These gold (RAM-SERS-Au-5) SERS substrates allow you to make fast, repeatable SERS measurements for SERS-active analytes.

Point and Shoot Adapter The Point and Shoot (PS) adapter (comes standard in the IDR-MINI-2-KIT) is designed to be used with many different samples. These include materials on surfaces and materials in vials or bottles. With a simple twist of the PS collar it allows adjusts the position of the sample in relation to the Raman focal point to obtain the best signal to noise. The Raman focal point is 8mm from the front of the lens. By twisting the PS collar you can adjust the position of the Raman focus so that the laser shines through various thickness barriers to maintain focus on the sample.

When rotated counterclockwise (viewed from the sample end) it adjusts the sample position further away from the final lens of the PS adapter.

http://www.oceanoptics.com/

1: Introduction

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When rotated clockwise it adjusts the sample position closer to the final lens of the PS.

When the PS collar is placed against the sample the focal point of the system is adjusted to accommodate a container or surface of the sample using these steps. Be sure to remain at least 11.5 cm from the PS Adapter for safety from the laser.

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SERS Adapter The SERS Adapter enables you take measurements using the SERS gold substrates.

The SERS Adapter attaches to the Point and Shoot Adapter. Unscrew the blue threaded space from the PS Adapter and screw on the SERS Adapter as shown below:

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Chapter 2

Standalone Operation

Overview You can use the IDRaman mini 2.0 as a standalone device, or connect it to a computer and use it with OceanView software (available for an extra charge from Ocean Optics). This chapter describes the standalone operation. If you are using your IDRaman mini 2.0 with OceanView, see Chapter 3: Operating with OceanView Software.

Make sure the IDRaman mini 2.0 has power (two AA batteries are installed) before trying to use it.

Place a vial with the selected sample into the vial compartment. The door must be closed and latched before the sample analysis can occur.

Controls and Indicators The IDRaman mini 2.0 features a user-friendly touch screen display for taking and viewing measurements in autonomous operation. The screen controls and indicators are described below.

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Screen Description

The initial power-up screen. Enter your 4-digit pin (the default is 1234).

Calibrate the device.

A calibration standard is shipped with every unit. Place the standard

vial in the sample chamber and press the Calibrate icon ( ).

The screen displays either Passed or Failed. If the calibration fails, tap the vial to settle the calibration sample and retry the calibration. Contact Ocean Optics if you are unable to calibrate the device.

The system is ready for a sample. Press to begin acquisition.


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Screen Description

The system is ready to fire. Press and hold the Laser icon ( ) to acquire a spectrum.

This match screen shows a spectrum of the sample (white trace) along with the best matching spectrum from the library (blue trace). The sample identification is displayed below the graph, and the match score shows at the top of the graph.

Press to save the result to the device.

System Information. Displays information about the system such as serial number, firmware version and the last calibration date.


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Screen Description

Press the Settings icon on the Laser Ready screen to display the first of the three Tools screens.

Tool Screen 1

-- Calibration

-- Library management

-- Change the PIN code

-- System information

-- Return to Laser Ready screen

-- Go to Tool screen 2

Tool Screen 2

-- Laser Raster

-- Save

-- Scan delay

-- Laser power level

-- Return to Tool screen 1

-- Go to Tool screen 3


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Screen Description

Tool Screen 3

-- Auto sleep time

-- Dynamic Raman Scattering

-- Return to Tool screen 2

-- Go to the next screen

Acquiring a Spectrum

► Procedure

1. Power on the device using the black power button on the side of the device.

2. Enter the access PIN number. The default is 1234, but you can change it (see Changing the PIN). The Laser Ready screen appears. The blue arrow on the right is used to start an acquisition sequence.

3. Insert the sample into the vial chamber or make contact with the sample using the point-and-shoot attachment (see Point and Shoot Adapter). The following screen appears to show that the laser is armed and ready for acquisition.

4. Press and hold the blue laser icon on the right to acquire the sample spectrum.

Once the acquisition has been completed, the sample spectrum displays as a white trace along with the best matching library spectrum (blue trace). The sample identification displays below the graph, and the match score appears at the top of the graph.


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Saving a Spectrum

Once you have acquired a spectrum, you can save it to the device.

► Procedure

1. Press the Save icon ( ) after acquiring a spectrum to save the spectrum to the device. A keypad screen opens.

2. Type a file name for the spectrum.

3. Press checkmark icon ( ) to save the spectrum with that file name.

4. If Auto Save is turned ON, the Save icon below the graph will be outlined in red, and each spectrum will automatically be saved with a file name of “Top Match + Date + Time”. This feature can be turned ON/OFF in the Settings screen (see Autosaving).


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Managing Libraries

This function allows you to view the libraries currently residing on the device, and disable and enable specific libraries based on your preferences. For more library functionality, see Operating with OceanView Software.

► Procedure

1. Press the Settings ( ) icon on the home screen. The following screen appears.

2. Press the Library icon ( ) to go to the following screen:

All libraries present on the device are listed with an (E) or (D) next to the library name, indicating whether the library is currently enabled (E) or disabled (D).

3. Click on a library name to display a screen similar to the following. Here you can choose to enable or disable a library. A library that has been disabled is not included in sample matching.

Calibrating the Device

Calibrate the IDRaman mini 2.0 using the calibration standard that came with the device.


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Note

The calibration standard is made from TiO2 (titanium dioxide) powder inside clear ABS plastic. The combined Raman spectra of these two materials produces a set of distinct peaks at both extremes of the IDRaman mini 2.0's wavelength range. This allows the device to be very accurately calibrated across its entire useful range. Check your calibration regularly to ensure the quality of your results.

► Procedure

1. Turn the power on for the device.

2. Press the Settings ( ) icon on the home screen. The following screen appears.

3. Press the Calibrate icon ( ) to go to the device calibration menu.

4. Place the calibration standard into the vial compartment. Once ready, press and hold

the Calibrate button ( ) to perform the calibration.

5. The Calibration results display:


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Passed – The calibration was successful. Continue with your measurements.

Failed – Make sure the calibration standard is clear of any debris and that is has been properly inserted into the vial compartment. Recalibrate the device.

Autosaving

Turning Auto Save ON allows the instrument to automatically save each acquired spectrum. Each spectrum will automatically be given a file name of “Top Match + Date + Time.”

► Procedure

1. Press the Save icon ( ) on the Home screen.

2. Select ON to turn the autosave function on. Or, select OFF to turn autosaving off.

When the Autosave function is on, the Save icon on the graph screen is outlined in red to show that it is inactive since saving occurs automatically.


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Scan Delay

Scan Delay (a delayed start) is used to protect you from potential ignition of samples. It will allow time to retreat to a safe distance from the IDRaman mini 2.0 before the acquisition begins.

► Procedure

1. Press the Settings icon ( ) and use the right arrow ( ) to advance to the second Tools screen (see Controls and Indicators for more information).

2. Press the Scan Delay icon. The Acquisition Time Delay screen appears.

3. Use the white up and down arrows to select the desired delay time (30 sec, 1 min, 1.5 min, 2 min, 2.5 min, 5 min, 10 min, or 20 min).

4. Press the left (back) arrow ( ) when finished. Laser Armed screen appears. The start delay begins after the system is armed and the laser is fired. The scan delay countdown time appears on the screen.


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You can abort the acquisition by pressing left (back) arrow ( ). The screen flashes green when the countdown and acquisition are finished. Tap the screen to view the graphical display.

Setting the Laser Power

There are 5 levels of laser power available from 10 mW to 50 mW.

► Procedure

To set the laser power,


2. Press the Laser Power icon ( ). The Laser Power Level screen appears.

3. Use the white up and down arrows to select the desired laser power level.

4. Press the left (back) arrow ( ) when finished. The laser power selected appears in the center of the laser icon on the Laser Armed screen.


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Setting the Laser Raster

Caution

Turning the laser raster OFF increases the risk of exogenous materials igniting. Use extreme caution.

► Procedure


2. Press the Laser Raster icon ( ). The following screen appears.

3. Select to turn the laser raster ON or OFF. The, press the left (back) arrow ( ).

If the laser raster is set to ON, the icon will display on the Laser Ready screen:


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Setting Sleep Delay Time

The IDRaman mini 2.0 has a built-in feature that places the device in sleep mode after a certain period of time to preserve battery life.

Note

It is recommended that the Sleep Delay not be adjusted from the factory setting to maintain optimum battery life.

► Procedure

To change the sleep delay time,

1. Press the Settings icon on the Laser Ready screen.

2. Press the Right Arrow icon ( ) twice go to the third Settings screen.

3. Press the Sleep Delay icon. The Sleep Delay Time screen appears.


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4. Use the white up and down arrow icons to adjust the sleep delay time in minutes (2, 3,

4, 5, 10, 20, 30). Press the Left Arrow icon ( ) when finished to set the sleep delay time.

Changing the PIN

The IDRaman mini 2.0 device is protected with a PIN. The default is 1234. This procedure allows you to change it.

► Procedure


2. Enter the current pin (the default is 1234) and press ( ).

3. Press the Settings icon on the Home screen to access the Settings screen.

4. Press the Lock icon on the Settings screen.

5. Type the current PIN number (the default is 1234) and press Checkmark icon ( ).


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6. Type the new PIN and press Checkmark icon ( ).

7. Repeat Step 6 to confirm the change.


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Chapter 3

Operation with OceanView

IDRaman mini 2.0 Start-up with OceanView Software You have the option of using your IDRaman mini 2.0 with OceanView operating software to provide additional functionality over operating in standalone mode. OceanView is available from Ocean Optics for an additional charge. This chapter describes how to use the IDRaman mini 2.0 with OceanView.

Start-up with Windows 7, 8 and 10

► Procedure

1. Turn on the computer.

2. Ensure that OceanView software is installed.

3. Be sure that power is supplied to the device. Turn on the IDRaman mini 2.0.

4. Connect the IDRaman mini 2.0 to the computer via a mini USB cable. Wait for the driver to automatically install (this may take several minutes). If the drivers don’t automatically install, see the Why can’t my computer locate the drivers FAQ on the Ocean Optics website.

http://oceanoptics.com/faq/drivers-not-loading-on-windows/

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5. Launch OceanView software.

Operating with OceanView Software OceanView software is available from Ocean Optics to acquire spectral measurement data from your IDRaman device. This section describes how to use the features available for your IDRaman mini 2.0. For more information on OceanView software see Product-Related Documentation.

Be sure to turn the power on to the IDRaman mini 2.0 before starting OceanView so that OceanView recognizes the device.


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The Acquisition Group Window provides the following functions for the IDRaman mini 2.0:

Control Function

Take continuous acquisitions. It is best to use small integration times (1.0 second or less) when using the ‘Continuous’ function for focusing. See Acquiring a Spectrum. This control is not recommended for systems that use a laser due to laser safety issues.

Take a single acquisition. Every time this control is activated, the laser fires and a measurement is captured.

Pause acquisitions.

The integration time is the number of seconds that the laser is firing and exposed to the sample. This number is roughly proportional to the peak intensities. Select the desired integration time and units (µm, ms, or seconds). The default is 100 ms.

Check Auto for auto integration. This setting sets integration automatically by waiting for the peak to hit 35000 counts. Maximum auto integration is 8 seconds.

Enter the number of scans to average. Click to stop averaging. See Acquiring an Average.

Select the units for the x-axis, Ramanshifts or Pixels on the detector (useful for calibration only).

Check the box to enable a reference spectrum. See Acquiring a Reference.

Check the box to enable the Clean Peaks function. See Peak Cleaning.

The instrument laser power can be adjusted by changing the value inside this box. The highest value represents full laser power, while a value of “0” means no laser power.

Adjusting laser power is particularly useful for analyzing samples that are susceptible to burning at higher powers. Select the laser power level (0 to 5), where 0 = off. Settings 1 through 5 correspond to laser power in tens of mW (5 = 50 mW, 4= 40 mW, etc). Laser power is accurate to within 3 mW.

Check this box to enable raster orbital scanning (see About Raster Orbital Scanning (ROS)). Laser rastering will cover an area of 1-2 mm on the sample

Check this box to match the sample’s spectrum to data in one or more libraries.


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Control Function

This table presents a list of libraries available on the device.

Enabled – The library is included in the sample matching

Licensed – These are libraries that have been purchased

Download samples from device – Download samples from device to specified directory.

Licensed Libraries – Unlock encrypted libraries with an access file that you purchased.

Acquiring a Spectrum

Set your integration time to the desired rate, choose your x-axis units, select the laser power,

and then click to acquire a single spectrum. Spectra can be adjusted by averaging scans, enabling reference spectra, and using the Clean Peaks function. See Acquire a Spectrum for information on acquiring a spectrum when working with libraries on the device.

Acquiring a Reference

OceanView allows you to take a reference measurement for a set integration time. The reference spectrum takes a measurement with the laser off, and then turns laser on and takes the Raman measurement. This lets you exclude ambient light and other environmental background as well as any fixed pattern noise from the CCD.

Note

Collecting a background reference will double the analysis time for any given integration time specified. Turning this this function OFF can speed up analysis times, but this should only be done when ambient light interference is not a concern.

Acquiring an Average

Averaging is used when your sample has a high background. Often samples will produce some or even large amounts of fluorescence. The quality of a spectrum is determined by the signal-to-noise ratio. The noise is determined by “shot noise” which is equal to the square root of signal. This means that samples with large backgrounds will inherently have poor signal-to-noise ratios. Increasing the integration time will improve the signal-to-noise ratio, but with high background samples this may be impossible due to the detector’s limited dynamic range.

Averaging allows you to take multiple short acquisitions and average them to improve signal to noise. Until the detector becomes dark noise limited, the signal to noise ratio will improve by the square root of the number of averages.


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Select the number of scans to average using OceanView.

Peak Cleaning

Clean Peaks is a beta correction algorithm that takes the measured Raman spectra and removes the baseline, removing the effect of fluorescence. This method is not appropriate for every sample, but when spectral backgrounds are a problem it can provide background free spectra.

Raw spectra and cleaned spectra can be displayed on the same graph using the Schematic View (Advanced Math Filtering Clean Peaks).

Example of Clean Peaks Node Used in the Schematic View


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Note

Checking the Clean Peaks Enable checkbox produces a single signal on the graph view showing just the clean peak processed spectra. Using this function in the schematic view allows you to view both the original AND the cleaned spectra.

Saving Data

Use following OceanView controls to save your data to an ASCII text or .spc file:

Control Function

Save graph to file. Saves your data as an ASCII text file unless you configure a different format using the Configure Graph Saving button described below.

Save content over time to one or multiple files (depending on how it is configured). OceanView provides the capability of saving and exporting processed data for each graph view to an ASCII file. When combined with external triggering, this feature can be used to automatically save full spectra timed to one or more external events. After this is selected once, press the spacebar for additional saves. Refer to the OceanView Installation and Operation Manual for more information (see Product-Related Documentation).

Device Libraries

The IDRaman mini 2.0 includes a preloaded, editable library of about 30 Raman spectra. Additional libraries of up to 9,000 spectra are also available for purchase. You can create new libraries, add and delete samples from a library, rename samples and send libraries to other devices using the IDRaman mini 2.0 with OceanView software.

Acquisition Set-up

In general, when you first attach the IDRaman mini 2.0 to your computer and start OceanView, a blank graph displays since no measurements have been taken yet. In the Acquisition window, every option is enabled (checked) except for Matching. Check this box to match your acquisitions to your onboard libraries.


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Next, you’ll want to select the file format and the target directory where you’ll save your sample measurements.

► Procedure

1. Select the File Writer icon ( ) to display the File Writer window.


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2. On the File Writer window, select Library (JSON) for the file format.

3. Set the Target Directory for saving your sample acquisition data.

4. Click Apply.

Acquire a Spectrum

► Procedure


2. Enter the access PIN number. The default is 1234, but you can change it (see Changing the PIN).

3. Insert the sample into the vial chamber or make contact with the sample using the point-and-shoot attachment (see Point and Shoot Adapter).

4. Click to take a single acquisition. This is the recommended control for devices

using a laser. The laser icon turns red while the laser is firing ( ).

Your spectrum graph appears. If libraries are enabled (checked), the sample match appears on the top-right corner (in the example below, the match is Propanol at 98%).


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Add Samples to a Library

You use your IDRaman mini 2.0 to scan samples to identify them based on their spectral properties. Once you save some spectra samples, you can add these samples to existing libraries or create a new library to save the samples to.

► Procedure

1. In the Saved Data panel, right-click on the sample to be saved.


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2. Select Add to Library.

3. Select the library to add the sample to.

or

Select New to create a new library. See Add a New Library for more information.

Rename Samples

Samples are stored as either .rmn (Raman) or .txt (text) files with numerical file names such as r_2010001_14-35-49-362 using the serial number of the device by default. You can change these file names to something more meaningful.

► Procedure

1. In the Saved Data panel, right-click on the sample name to change.


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2. Type in the new sample name in the Change Sample Name window.

3. Click OK to save it.

Delete Samples from a Library

You can delete samples from a library stored on the device.

► Procedure

1. In the Libraries panel, click on the library containing the sample to be deleted.


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2. Right-click on the sample to be deleted.

3. Select Delete.

Add a New Library

Libraries can be added to the list of existing libraries on the device. The tasks to do this include naming the library and uploading it to the IDRaman mini 2.0.

► Procedure

1. Create a new library when measuring a sample by right-clicking on New.


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2. Select Window | Library to display the Library window to view the libraries created.


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Upload a Library to Another Device

You can send a library on your IDRaman mini 2.0 to another device.

► Procedure

1. From the Library window, check the enable box of the library (or libraries) to be uploaded.

2. Select Upload to Device.

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Appendix A

Specifications

Specifications Criteria

Raman Spectrum Range 400 to 2300 cm-1

Spectral Resolution 12 to 14 cm-1 (FWHM) across spectral range

Laser (excitation wavelength) 785nm±0.5 nm, 2 cm-1

line width, stability <0.1 cm-1

Laser Output Power 100 mW, 70 mW at the sample

Collection Optics NA = 0.50, 8mm working distance; 0.2 to 2.5 mm spot size

Signal to Noise Ratio (SNR) SNR = 1000; 70 mW toluene 0.1 second integration

Exposure Automatic modes (10 ms minimum)

Battery 2 AA batteries (6 hours w/Alkaline, 11 hours w/Lithium)

External Power Supply Not applicable, replaceable batteries used, USB power available

Weight 11.75 oz. (0.33kg)

Dimensions 3.6 x 2.80 x 1.5 in. (9.1 x 7.1 x 3.8 cm)

Operating Temperature -20°C to +40°C (continuous)

Display QVGA high visibility, glove compatible, color touch screen

Enclosure Rating IP-40

Security Log-in PIN Code, 4 digit

Measurement Accessories Vial holder, Point and Shoot

Compliance CE certification (IEC 60825-1, EN 61326-1)

Collection Techniques Raster Orbital Scanning (ROS) to reduce explosive ignition risks and narcotic sample damage. Dynamic Raman Scattering (DRS) technology for low level Raman code detection.

Safety Class 1 laser safety with vials

Class 3B with Point-and-Shoot accessory

A: Specifications

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Index

A accessories, 4 acquire spectrum, 11 acquiring

average, 26 reference, 26

autosave, 15 average

acquiring, 26

B box contents, 3

C

calibrating, 8, 13 Clean Peaks, 27 controls, 7

D

document audience, iii purpose, iii summary, iii

F

features, 1

I IDR-MINI-2-KIT, 3 indicators, 7 introduction, 1

L laser armed, 9 laser power

set, 17 laser raster

set, 18 laser ready, 8 laser safety, B libraries

manage, 13 library, 28

acqiuire a spectrum, 30 acquisition set-up, 28 add new, 34 add samples, 31 delete samples, 33 rename samples, 32 upload to another device, 36

library match screen, 9

M match screen, 9 models, 2

O

OceanView, 4, 23 acquiring average, 26 acquiring reference, 26 acquiring spectrum, 26 Clean Peaks, 27 operation, 24 save data, 28 startup with Windows 7, 8 and 10, 23

overview, 1

Index

40 000-40000-011-02-201601

P pincode, 8

change, 20 Point and Shoot Adapter, 4 product-related documentation, iii PS Adapter, 4

R

Raster Orbital Scanning (ROS), 2 reference

acquiring, 26 remote interlock, C

S safety

laser, B notices, A remote interlock, C

safety notices, A save data, 28 scan delay

set, 16 SERS Adapter, 4, 6 SERS substrates, 4 sleep delay

set, 19 specifications, 37 spectrum

acquire, 11 save, 12

system information, 9

T Tool screen, 10

U upgrades, iv

W warranty, iv what's new, iii