Illustra ™triple Prep kit: Get your DNA, RNA and protein from one sample

Market potential: In all molecular biology and protein laboratories DNA, RNA and proteins are isolated and purified routinely from the same sample using three different protocols. In larger labs, isolation and purification of samples can run up to more than 100 samples at a time using up a significant portion of the day and other laboratory resources.

GE Healthcare Life Sciences ‘Illustra ™triple Prep kit can perform all three isolation and purification from the same sample.

Benefits:

1. Saves a significant amount of time which can be devoted to other important areas of research.2. As all the isolation and purifications are done from a single sample, the data obtained for DNA,

RNA or protein correlation is consistent. 3. The amount of sample required to perform the experiment is small. Thus it utilizes hard to replace

samples more effectively or is especially valuable if the samples are in short supply.4. It also allows for better quantification of expression levels of DNA, RNA and proteins thus

making it a valuable tool for expression profile reading experiments. 5. Under similar experimental conditions, Illustra ™triple Prep kit showed comparable or better

level of yield and purity compared to conventional single analyte purification kits.

Procedure: Illustra ™triple Prep kit uses similar protocol comparable to existing spin column type purification kits. Tissue samples are disrupted and homogenized and is mixed directly to optimized lysis buffer provided with the kit. The sample at this point can be stored up to six months in -80°C if required processing at a later date.

DNA and RNA isolation: DNA is first isolated passing the lysate through a DNA binding spin column. The flow through is retained and further processed to isolate RNA by transferring it to a RNA binding spin column. The Flow through is further collected to isolate protein. The RNA sample is further purified by treating it with DNAse I provided with the kit. Both DNA and RNA binding columns are washed with provided wash buffer and eluted in separate microfuge tube. The total procedure does not take more than ten minutes each. The flow through from the RNA binding column is mixed with a precipitation agent and spun to pellet the precipitated protein. Protein sample is then washed with water and resuspended in 2D-DIGE or 7M urea.

Purity and Reliability: Compared to conventional commercially available single analyte purification kits, Illustra ™triple Prep kit returns consistently higher and pure yields. Downstream analysis showed thus purified DNA, RNA and protein samples are compatible with end-point PCR, sequencing, Real time PCR, microarray analysis, SDS-PAGE, western blotting, 2D-DIGE and LCMS.

Conclusion: Illustra ™triple Prep kit provides real advantages compared to the currently available purification kits in terms of time required performing the experiments and also the amount of sample required to perform the experiments. Furthermore it also advantageous in terms of better consistency of data obtained and higher and purer yield of the samples.