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Image-Pro Premier Basic Training Course. Part 7 - Image Enhancements. Image Enhancements. Enhancements can be made for several reasons: Aesthetics Functionality Practicality If the image is modified, the process must be: Systematic Justifiable. - PowerPoint PPT Presentation
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Image-Pro PremierBasic Training Course
Part 7 - Image Enhancements
Image Enhancements
Enhancements can be made for several reasons:AestheticsFunctionalityPracticality
If the image is modified, the process must be:SystematicJustifiable
Always make a copy of the image before
processing!
Display Enhancement
Use Look-Up Tables to adjust how pixels are displayed on the screenNon-destructive unless appliedDoesn’t generally improve ability to analyse images
Filters
Process individual pixels based on their environmentUsed to:
enhance or reduce noisePull out EdgesEnhance morphological feature
Masks
Create binary images for processingCan be used to extract further information from objectsUse from Count/Size to fill holes or add smoothing
Colour Extraction
Colour increases the complexity of analysis
Colour isn’t always necessary or useful
Colour images can be converted to mono from the Adjust ribbon by either:
Extracting colour channels
Averaging information from all channels
Colour Extraction
Mono Average
Colour Separation
Background Correction
Uneven background can be aesthetically jarring and render analysis difficult
Use background Correction to flatten backgrounds by:
Subtracting background image
Estimating background
Colour Composites
Fluorescence imaging is typically monochromeMultiple images are taken separately from the same sampleColour composites recombine multiple monochrome images into a single workspace