Immuno First _ Past Years Qs

7/31/2019 Immuno First _ Past Years Qs

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Part#1Questionsandanswers:1- Calcenurin is involved in the activation of

T cells

2- Calcenurin is inhibited by Cyclosporin

3- Rheumatoid factors are directed against

Allotypic antibodies

Be sure to know the following. We had good questions about these topics but I dont remember the

m:

1- differences between primary and secondary immune responses.

2- The concept of bystander activation

3- markers of different cell types especially newly discovered cells like NK-T cells.

1)Whichofthefollowinglymphocyteshasthehighestconcentrationinblood:a- CD4+ CD8-

b- CD4- CD8+

c- CD4+ CD8+

d- CD4- CD8-

2)Whichofthefollowingvaccinesisadministeredorally:a-pollo-salk

b- polio sabin

c- smallpox

d- hepatitis B

e- a+b

3)WhichofthefollowingisnotapartoftheIggenesuperfamilya- Ig heavy chain

b- T cell receptor a chain

c- B cell receptor (signal transduction)

d- B + C

e- None of the above

4)WhichofthefollowingIgshasthelowestserumconcentration:a- A b- G c- M d-D e- E


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5)WhichofthefollowingIgscancrosstheplacenta:a- A b-G c-M d-B+Ce- all of the above

6)HyperIgMsyndromeisduetoadefectin:a- CD40L b- CD40 c- B cell receptor

d- T cell receptor e- M chain7)WhichofthefollowingisrequiredinTcellmaturatuina- IL 1 b- IL 4 c- IL 5 d- IL 7 e- IL 9

8)Hereditaryangloedemaisduetodeficiencyof:a- C4 binding protein b- C1 inhibitor c-C1

d- C4 e- None of the above

Answers:

1-a 2-e 3-e 4-e 5-b 6-a 7-d

8-b

10)Allofthefollowingplayaroleinpromotingtheprocessofapoptosisexcept: bcl2 activation

11)AmemoryBcellhasallofthefollowingmarkersonitssurfaceexcept: IgM (it has only the switched one IgG)

12)Allthefollowingcellshasanantigenreceptorontheirsurfaceexcept: Plasma cell (its function is only producing antibodies)

13)InscreeningforeffectivebonemarrowtransplantationwithhematopoieticstemcellwhichofthefollowingmarkersshouldbetargetedasthecellscanbeidentifiedbytheFACSmachine: CD34

14)WhichofthefollowingisthecrucialstepintheADCCmechanismofcellkilling? the presence of FC gamma receptor on the killer cell.

15)Whichofthefollowingimmunemechanismsutilizesmannanbindinglectinintheirkillingprocess? Complement system

16)InwesternImmunoblottingtechniquehorseradishperoxidase(HRP)enzymeisboundto

whichofthe

following

secondary antibody

17)Whichofthefollowingcellsfailtohometosecondarylymphoidorgansbecausetheylackhomingadhesionmolecules? The memory cells ( they go to blood and tissue when ----- cells go to secondary lymphoid organs)


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18)WhatisthebestimmunecenariooftwodifferentepitopesAandB,enteringthebodyforthefirsttime?


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Predetermined A specificity and predetermined B specificity alone (clonal selection theory, there

is no

receptor on B cell which has 2 specificities at the same time for the two epitopes)

1)Whichofthefollowingevasivemechanismisusedbyherpesvirusesinordernottobedet

ectedbytheimmunesystem Interfere with the synthesis of class I MHC Ag inside the cell.

2)IfanHLA-B1cellistransplantedinanHLA-B5fetusduringtheembryogenesisperiod,whichofthefollowingscenariosisgoingtohappen During embryogenesis period there is a development of T-cell, So any cell is going to be recogni

zed as

foreign, and when we transplant B1 cell at embryo at the time of embryogenesis or development then it

will be recognized as self, so the anti-B1cells in the thymus will be deleted and B1 will be accepted.

3)Allofthefollowingareconsideredasphagocyticcellsexcept the natural killer cells

4)WhichofthefollowingImmunoglobulinsfailetoagglutinatespecificantigenicdeterminntonthesurfaceofcell IgM treated with a papaine enzyme

5)Inthesynthesisofmonoclonalantibodiesthehybredomaconsistof Specific B cells fused with non specific myeloma cells

6)WhichofthefollowingImmunoglobulinshasareceptoronthesurfaceofNKcells IgG

7)Intheprimaryimmuneresponsethegenerearrangementoftheheavychainstartswithth

ebindingof

D to J

8)SomaticHypermutationinvolvestheproductionofareceptorofhighaffinitythisinvolves:

the v region of the heavy and light chains and the region of the alpha and beta chain.

9)Superantigens: Bind to B chain of the T cell receptor and to class II MHC

27)AnewbornbabyhasclassIMHChaptotypeas:A1A3 B5B10 C1C9,borntoamotherwhohasahaptotypeofA2A3 B7B10 C4C9,whichofthefollowinghaptotypescouldbethefatherofthisbaby? A5A1, B5B8, C1C5 ( the baby inherit one from the father and one from the mother, it is a codom

inant


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type of inheritance {i.e. the baby has A1A3 then A3 from the mother and A1 from the father}

28)RestrictioninAgpresentationinvolves: T cell receptor recognises non-self Ag in association with self MHC.

29)PolymorphismamongtheMHCallelicformsinvolvesthestructuralareaof: The a1,a2 domains in class I (the area of Ag presentation)

30)Duringthesecondaryimmuneresponseclassswitchinginvolvestheformationofanyoftheseimmunoglobulinsexcept:

IgM, IgD


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31)ITAMS(immunereceptorTyrosin-basedactivationmotif)arepresentinallofthefollowingchainsexcept

T-cell receptor (the B-chain of T-cell receptor)

32)CD19

:

- Is present on naieve B cell

- Could not be recognized in any T-cell subset- Can be targeted as B-cell marker for the FACS machine all of the above

19)Allofthefollowingproteinsarepartofthe(ptk)enzymesysteminvolvedinthesignaling

of

T-cellthroughITAMSphosphorylationexcept:

bcl2

20)ThecytoplasmicproteinneededfortheformationofacomplexwithcyclosporineainorderforthedrugtoworkbyfinallyinhibitingtheformationofIL2inT-helpercellis: The immunophilines (it bind to cyclosporine a to inhibit calcenurin from working)

21)Whichofthefollowinglymphokinereceptorisdefectiveinpatientwithseverecombinedimmunodeficiencysyndrome:

IL7, IL2, IL4

22)Amemorycellhasallthefollowingmarkersonitssurfaceexcept: IgM

23)Oraltoleranceismainlymediatedbybeingexposedtolargeoraldoseofanantigen,whichofthefollowinglymphocytesisinvolvedinthisprocess:Naieve T-vell from gamma,delta type24)WhichofthefollowingisorareinvolvedinreceptoreditinginordernottobindselfAgandformanautoimmunedisease?

the variable light genes in T-cell receptor

25)InnegativeselectionofT-cellsinthymusglandoccursduringcelldevelopmentofwhichofthefollowingstages the double positive stage

26)Allofthefollowingplayaroleinapoptosisexcept: Bcl2 activation (repeated question)

MATCHING:


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- Multiple sclerosis increased TH1 Activity

- Toxic shock syndrome Super antigen

- CD80 CD28

- CD40 CD154

- ICAM

LFA1- LCK CD4

TRUEAND FALSE

1)Ifanantibodyiscrossreactivewithanantigen,itimpliesthatitdoeshavealowersensitivity

comparedtoanantibodythatisnotcrossreactive. False (cuz sensitivity will not be affected by cross reaction, but the specificity will be lower, because

we

are talking about false positive)

2)Diversityinthelightchain(lambda)is4timesmorethanthatlf(kappa)chainbecausewehave4differenttypesoflambda False (the constant region has nothing to do with diversity)

3)Alteredpeptideligandvaccine,althoughitsometimescouldleadtoreceptoractivationbutit

provides

suppressionofT-helpercellsinsteadofproliferationanddifferentiation

TRUE4)Ifthethymusglandisremovedimmediatelyafterbirth,Thenthebaby'sresponsetoviralinf

ectionwill

beimpaired. False (nothing will occur after removing the thymus gland unless we ------- the baby)

5)IfTH2isdiffectedthenIsotypeswitchingIgEwillbeaffected. True ( because TH2 provide B cell with IL2,I4,I5 responsible for isotype switching)

6)AllelicexclusionphenomenaimpliestheexpressionofoneallotypeonimmunoglobulinandTcellBchainonly.


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Part#21. An example of innate immunity is:

a. Antibody production by plasma cells.b. Antigen removal by cilia in the respiratory tract.c. Complement activation by antibody bound to the surface of a bacterium.d. Memory response to influenza virus.e. Recognition and killing of virus infected cells by cytotoxic T cells.Answer:b

2. Humoral immunity can be acquired passively by:

a. Catching a virus from a friend by shaking hands.b. Receiving a vaccine of influenza virus grown in eggs.c. Receiving serum from someone who recovered from an infection.d. Receiving leukocytes from an immune family member.e. Sharing a soda with someone who has a cold.Answer:c

3. The antibiotic penicillin is small molecules that dose not induce antibod

y

formation. However, penicillin binds toserum proteins and forms a complex that in some people induces antibodyformation, resulting in an allergicreaction. Penicillin is therefore:a. An antigen.b. A hapten.c. An immunogen.

d. Both a and be. Both a and canswer:d

4. For specific antigen recognition by T cells:

a. Antigen is bound a T cell membrane antibody.b. Denaturation of antigen dose not reduces epitope recognition.c. Developing T cells must be exposed to the antigen.d. MHC molecules are not required.

e. Soluble antigen is bound directly without processing


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answer:a

5. Effector functions of complement include all of the following except:

a. Attracting phagocytes to the site of infection.

b. Facilitating phagocytosis of complement coated

bacteria.c. Increasing blood vessels permeability to plasma proteins.d. Lysing bacterial cells.e. Presenting antigen to B cells.Answer:e

6. Antigen epitopes recognized by ______________ tend to be

____________.a. B cells; highly accessible sites on the exposed surface of antigen.

b. Cytotoxic T cells; highly accessible sites on the exposed surface of theantigen.c. Complement; highly accessible sites on the surface of APC.d. Helper T cells; associated with MHC class I on infected cells.e. Macrophages; associated with MHC class II on APCs.

7. Which statement about antigen epitopes is false?a. An epitope may be shared by two different antigens.

b. A protein molecule usually contains multiple epitopes.c. B cells bind only processed antigen epitopes.d. Epitopes may be linear or assembled.e. Some epitopes are more immunogenic that othersanswer:c

8. Phagocytosis:

a. Can be stimulated by antigen binding to complement or antibody.b. Is an antigen specific process.

c. Must be preceded by antigen processing.d. Rids the body of virus infected cells.e. Only occurs after plasma cells begin secreting antibodyanswer:a


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9. Jenner observed that milkmaids who were infected with cowpox werelater immune to smallpox infections. Thisan example of a(n):a. Acquired immunity of barriers skin cells.

b. Active immunization with a non releated

organism that causes similarsymptoms.c. Innate immunity of milkmaids to smallpox.d. Memory response to a cross reacti ve antigen.e. Passive immunization from contact with cows milk antibodies.Answer:d

10. ELISA:

a. Detect agglutination of cells by antibodies specific for cell surface

antigen.b. Uses isotope labeled antibodies to detect extremely small amounts ofantigen.c. Is used to isolate cell populations or proteins (including antibodies).d. Uses enzyme linked anti IgG to detect serum antibody to specificantigen.e. None of the above.Answer:d

11.Allspecificlymphocyteshavesurfacemoleculesthatserveasspecificreceptorsfortheantigenforwhichthatlymphocyteisspecific.InthecaseoftheB-lymphocyte,thissurfacereceptorhasamolecularconfigurationthatisvirtuallyidenticaltowhichoneofthefollowing?a. A cytokine.b. An MHC molecule.c. The antigen for which that B lymphocyte is specific (known ascomplementary configuration).

d. An antibody molecule.Answer:d

12. B cell maturation occurs in:

a. The thymus.b. The bone marrow.c. The lymph nodes.


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d. The spleen.e. The peyers patches.Answer:b

13. Antibody cross reactivity is demonstrated by antibody binding to:

a. Cell surface marker.b. A hapten.c. A hapten carrier complex.d. An antigen that is structurally similar to the immunogen.e. The immunogen.Answer:d

14. Which of the following is an example of passive acquired immunity?

a. Antibodies secreted in breast milk.b. Anti snake bite serum.c. A tetanus shot.d. Antibodies genertated by catching a cold.e. Antibodies transferred through the placenta.Answer: b

15. A component that facilitates phagocytosis when bound to amicroorganism:a. Antigen.b. KDOc. Capsuled. Opsoninse. bactoprenolanswer: d

16. immunoglobulin classes may not be distinguished by the type of:a. Light chain they possess.b. Carbohydrate on their light chain.c. Constant regions in their light chain.d. Heavy chains they posses.e. None of the above.


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Answer: a

17. The constant regions in heavy chains determine:

a. Fc receptor binding.

b. Conformation of the binding site to the epitope.c. Affinity of the complement receptors.d. Interaction of the Fab with cytokines.e. None of the above.Answer: a

18. Serum from an AB positive, Rh negative patient agglutinates red blood

cells from a patient who is Rh negative.What is likely blood type is the second patient?a. It cant be determined from the information provided.b. Type Ac. Type Bd. Type Oe. Type ABanswer: a

19. In the early stage of a primary immune response, dendritic cells prese

nt

antigen to helper T cells. In this process,to which protein are antigen fragments bound?a. Membrane IgM & IgD.b. IgG bound to plasma membrane FcR receptor.c. IgE bound to plasma membrane FcR receptor.

d. Class I MHCe. Class II MHCanswer: e

20. Breast fed infants have fewer intestinal infections than do infants fedon cows milk, because of


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protection provided by antibodies in breast milk. Of what isotype are themajority of these protectiveantibodies?a. IgA

b. IgGc. IgMd. IgDe. IgEanswer: a

21.doesn'tfixcomplementa)IgG2

b)IgG3

c)IgG4d)IgG1answer:c

22.eliminationofbacteriabyopsonizationa)neutralizationofbacterialtoxin

b)recognitionofbacteriaboundantibodiesbyFcreceptoronmacrophagec)preventionofbacteriaadhesiontoendothelialcelld)bindingantibodiesFconbacteriae)class1MHCantigen

answer:b

23.MHCIandMHCIIa)Constituvelyexpressedbymostcellsinthe

b)expressedbyMacrophageanddentriticcellc)Constituvelyassociatedhaptend)ExpressedINFgamma28)InjectingComplementCkillingantigen-AbinpatientlacksC3Answer:b

24.WhichonehasJchain?a)IgD,IgA2

b)IgG2,IgEc)IgE,IgMd)IgG3,IgA1e)IgA1,IgM


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answer:e

25.NotfoundonmaturerestingTcella)CD5

b)MHCIIc)MHCId)CD2e)CD3answer:b

26.Recognitionreceptorfunctionsassecretedreceptora)Mannanbindinglectin

b)CD14c)Tolllikereceptor

answer:a

27.Abdestructionbypepsinyieldsa)(F(ab'))2fragments

28.Whichiswrongconcerningepitopes?a)Epitopeisalwaysimmunogenic

29.AllelicexclusionEXCEPTa)OnoneBlymphocyteyoumayfindbothaandbheavychains

30.Immunogenicmeansa)Stimulatestheimmunesystem

31. In a non-emergency situation, a patient of blood type A requires

transfusion of plasma. Person ofwhich ABO types would be regarded as compatible donors?A. Either A or O.

B. Either B or O.C. Either B or AB.D. Either A or AB.E. Either AB or O.Answer:a


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32. In an immune response immunoglobulin genes undergo somaticmutation. What is the goal of thissomatic mutation?A. Increase in antibody affinity.

B. Antibody class switching (for example, from IgM to IgG).C. Allow a single b cell to produce antibody of several isotypes.D. Change in type of light chain produced (from kappa to lambda or thereverse).E. Production of anti-class IIMHC antibodies.Answer: a

33. The blood bank lab report indicates that a patient is Rh-positive. Whic

h

interpretation of thesestatements is most accurate?A.ThepatientsplasmacontainsIgGantibodiestotheRh0(D)antigen.B.ThepatientsplasmacontainsIgMantibodiestotheRh0(D)antigen.C.ThepatientserythrocytescarrytheRh0(D)antigen.D.ThepatientserythrocyteslacktheRh0(D)antigenAnswer:c

34.Inthelaboratoryaserologicalreagent,preparedfromserumofasingle

donor,wasfoundtobindamultivalentantigenwithhighaffinity,cross-linkantigentoformprecipitate,and,whenboundtoantigen,activatecomplement.Whichphrasewouldbestdescribethestructureofthisreagent?a.FabfragmentofIgG.

b.FcfragmentofIgG.c.(Fab)2fragmentofIgG.d.IntactIgG.Answer:d

35.Whatprocesswasmostresponsiblefortheincreaseinantibodyaffinityandavidity?a.RandomsplicingofV/D/Jsegmentsofheavy-chaingenesandv/jsegmentsoflightchain.

b.Excisionofconstant-regionexonsfromDNA,sothatassembledheavy-chainvariable-regionexonsbecomesadjacenttoconstant-regionexonsofdifferentDNAsequence.


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c.RandomadditionofnucleotidesatV/DandD/Jsplicejunctionsofheavy-chaingenesandV/Jsplicejunctionoflight-chaingenes.d.Mutationsofvariabledomainexonsofheavyandlightchains,followed

byselectionfortheabilitytobindepitopes.

Answer:d

36.J-chainsarenotassociatedwith:a.IgG.

b.Polymericimmunoglobulins(morethantwoFabs).c.SecretoryIgA.d.IgM.

e.Noneoftheabove.Answer:a

37.Activelyacquiredimmunityisnottheresultof:a.Transferofbonemarrowfromoneindividualtoanother.

b.Immunizationwithavaccine.c.Exposuretoanindividualwhohasaninfectiousdisease.d.Aphysicianadministeringagammaglobulinshottosomeonewhohashadaneedlestick(immunoglobulins).

e.A&Danswer:d

38.IgDantibodyfunctionsas:a.Antigenreceptorsonimmaturetcell.

b.AnADCCreceptorsofNKcells.c.AhormonereceptoronNKcells.d.Atriggerofthecomplementcascade.

e.Noneoftheabove.Answer:e

39.Antibodyaffinityisprimarilydeterminedbytheaminoacidsequencein:a.Theconstantregionsoftheimmunoglobulinmolecule.

b.Thevariableregionsoftheimmunoglobulinmolecule.c.TheFcoftheimmunoglobulinmolecule.


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d.Thej-chain.e.Noneoftheabove.Answer:b

40.Theantigenpresentingcell:a.Maybeadendriticcellintheskin.

b.IsnotaTcell.c.Producescytokineswhichinfluencetheadaptiveresponse.d.ProcessesantigenpriortopresentationtotheTcell.e.Alloftheabove.Answer:e

41.NKcellsrecognizetumorcellsusing:

a.Patternrecognitionreceptors.b.Antibody-likereceptors.c.Complementreceptors.d.Ahomingmechanism.e.Noneoftheabove.Answer:a

42.Afterisotypeswitching,IgMandIgGcanbeproducedinwhichvariabledomainsofheavyandlightchains,andconstantdomainsoflightchains,areidentical,butconstantdomainsofheavychainsdiffer.Whichstatement

belowaboutsuchpairsofantibodiesismostlikelytobetrue?a.IgMwillhaveloweraffinitythanIgG.

b.IgMwillhavehigheraffinitythanIgG.c.IgMwillbecapableofactivatingcomplement,butIgGwillnot.d.IgMmoleculeswillbesmallerthanIgGmolecules.e.IgMandIgGwillmoleculeswillbinddifferentepitopes.Answer:c

43.ManyisolatesofthebacteriumNeisseriameningitidescanproducemucosalinfectionsbutcannotsurviveinthe

bloodstream.Cellsofsuchbacteriaarekilledbycontactwithcell-freeplasmaorserum,evenfrompersonswhohaveneverpreviouslybeenexposedtoNeisseria.Whichmechanismismostlikelytoberesponsibleforthe


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killing?

a.Activationofcomplementbythealternatepathway.b.Activationofcomplementbytheclassicalpathway.c.BindingofIgG.d.BindingofIgM.e.BindingofIgA.Answer:a

44.OnceHchaingeneshavebeenproductivelyrearrangedandexpressedonthepre-Bcellmembrane,thenexteventtooccurinthecellis:a.Deathofcellsbindingselfantigen.

b.ExpressionofmembraneIgD.

c.ExpressionofmembraneIgM.d.Proliferationofthepre-bcells.e.Somaticrecombinationoflightchaingenes.Answer:e

45.PepsindigestionofanIgGantibodyagainsttetanustoxoidwill:a.Resultinlossoftheabilitytoformalatticewiththetoxoid.

b.ProducetwoFabmolecules&oneFcfragmentc.

Produce

a(Fab)2

molecule

&

loss

of

the

ability

of

the

Fc

fragment

to

bind

tomacrophages.d.LossoftheLchaine.LossoftheC1heavychainconstantdomainanswer:c

46.ThemostimportantIgprotectinga1-month-oldbabyis:a.IgM

b.IgGc.IgA

d.IgEe.IgDanswer:b

47.Anadvantageofadaptiverecognitionoverinnaterecognitionisthatit:a.Faster

b.Cannotresultintherecognitionofthebody'sowntissues


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c.Triggersaninflammatoryresponsed.Targetsspecificstrainsofpotentialpathogense.Requireslessenergytoproducethedefensiveresponseanswer:d

48.Duringheavychainsplicing,N-nucleotidesadditionoccursin:a.ThejunctionbetweentheJregion&theconstantregion.

b.ThejunctionbetweenV&D&betweenD&Jregion.c.thenucleotidesbetweentheexonscodingforthemembrane-spanningregionoftheconstantchainandtherestofthechain:d.A&Babovee.A,B&C

49.Earlyinducedimmuneresponsesarelikeadaptiveimmunityinthatthey:a.Areantigenspecific.

b.Demonstrateimmunememory.c.Involvemacrophages&complement.d.InvolveT&Blymphocytes.e.Usepresynthesizedproteinsthatcanbereleasedquicklyuponcellactivation.

Answer:e

50.Severecombinedimmunodficiencydiseaseismostlikelytoexhibitthecharacteristic(s):a.AgeneticdefectintheNADPHoxidasesystem

b.Alossintheenzymeadenosinedeaminasec.Atriadofthrombocytopenia,eczema,&recurrentinfections.d.AnabsenceofTcells,hypocalcemia,&tetany.e.RequiresthechemokinesCCR-5frothediseasetoprogress.f.Adefectinthetransitionfrompre-BcellstoBcels.

g.AselectivelossinIgAlevels.

51.Whichofthefollowingstatementsaboutasecondary(memory)antibodyresponseisFALSE?a.ThesecondaryresponsecomesfrommemoryBcells.

b.Thesecondaryresponseisfaster.c.ThesecondaryresponseisprimaryIgM


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d.Thesecondaryresponseproducesmoreimmunoglobulinsthantheprimaryresponse.Answer:c

52.A20-year-oldcollegestudentworkingpart-timeinapediatricAIDSclinicdevelopsaviralexanthemwitharash.Herbloodisdrawn&testedforspecificantibodiestovaricella-zoster(chickenpox).Anti-varicellaimmunoglobulinbelongingtowhichofthefollowingantibodyclasseswouldindicatethatsheisimmunetochickenpox?

a.IgAb.IgD

c.IgEd.IgGe.IgManswer:d

53.AnIgG2moleculeiscomposedofwhichofthefollowing?a.Onealpha,onegamma2,&twokappachains

b.Onegamma1chain&twokappachainsc.

Two

gamma1

chains

&

one

kappa

&

one

lambda

chain

d.Twogamma1chains&twokappachainse.Twogamma2chains&twokappachainsanswer:e

54.ThesmallamountsofimmunecomplexeswhichforminhealthyindividualsarenormallyremovedinaprocessinvolvingFcreceptorsexpressedby:a.Macrophages.

b.PMNs

c.Bcellsd.RBCse.Mastcells

55.Theantigen-specificreceptorsofBlymphocytescontainonlyasingle:a.Polypeptidechain

b.Hypervariableregion


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c.Siteforantigenbinding

d.Constantdomain

e.Noneoftheabove

answer:e

56.Whichofthefollowingistrue?

a.Antibodiesarecomposedof4id

enticalpolypeptidechains.

b.Hypervariableregionsareprimar

ilyfoundontheFcportionofthe

antibodyprotein.

c.Tcellreceptor&membranebou

ndimmunoglobulinshaveidentical

structures.

d.Theantigenbindingsiteisfound

ontheFabregionofthe

immunoglobulinmolecule.

e.TheFcportionoftheTcellrece

ptorconfersantigenspecificity.

Answer:d

57.Thedetectionofhigh&sustain

edlevelsofamonoclonalantibody

inthe

serumofapatientmostoften

suggestsamalignancyinvolving:

a.Bonemarrowderivedlymphocyte

s(myeloma)

b.Placentaltrophoblastcells(chorio

carcinoma)

c.Thymusderivedlymphocytes(leu

kemia)

d.Stromalcellsofthelung(mesoth

elioma)

e.Liverhepatocytes(hepatoma)

answer:a

58.Superantigensare:

a.Bacterialpolysaccharides.

b.AbletoactivateallTcells.

c.Antigensthatdonotrequireproce

ssing&presentationforTcell

activation.

d.AbletoinduceBcelldifferentiati

onwithoutTcellhelp

e.Alloftheabove.

Answer:c


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**itincludechapters8&9**

1)TheMHC-encodedantigenreceptorsfunctionby:

a-Bindingantibodytotheantigendepot.

b-Bindingcomplement

c-EnsuringthatantigenpresentingcellsinteractwithT

cells

d-Aandc

e-Noneoftheabove

ans:c

2)Intheearlystageofprimaryimmuneresponse,dendritic

cellspresentantigentohelperTcells.inthisprocess,to

whichproteinareantigenfragmentsbound:

a-MembraneIgM&IgD.

b-IgGboundtoplasmamembraneFcRyreceptor

c-IgEboundtoplasmamembraneFcRereceptor

d-Class1MHC

e-Class2MHC

ans:e

3)HLAmolecules:

a-Discriminatebetweenself&non-self

b-PresentprocessedantigentoTcellsofthesameHLA

types.


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c-Arehighlypolymorphic

d-Controlsusceptibilitytocertainimmunologicdisorders.

e-Arecharacterizedbyeachoftheabove.

Ans:e

4)Class2HLAmolecules:

a-presentpeptidicepitopestoCD4+Thelpercells.

b-presentpeptidicepitopestoCD8+Thelpercells.

c-AreencodedbythegeneregionsDP,DQ,DR

d-Appearonthemembranesofmostnuclestedcells

e-Arecharacterizedbyeachoftheabove.

ans:c

5)therejectionofallograftcanbeslowedorprevented

by:

a-carefulmatchingoftheMHC-class1histocompatibility

antigensusingantibodiestoothecellsurfacemolecules.b-Bygraftingaprivilegedsite

c-Bytreatingtherecipientwithimmunosuppressivedrugs

d-Bypassiveimmunizationoftherecipientwith

antibodiesagainsttheMHCclass2antigensofthedonor

e-Allornoneoftheabove

f-A+bbutnotc+d

ans:e

6)MHC1andMHC2:

a-Constituvelyexpressedbymostcellsinthebody

b-Expressedbymacrophageanddendriticcells

c-Constitutivelyassociatedhapten

d-Expressed-INFgamma


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e-InjectingcomplementCkillingantigenAbinpatient

lacksC3

ans:b

7)NotfoundinmaturerestingTcell:

a-CD5

b-MHC2

c-MHC1

d-CD2

e-CD3

ans:b

8)HLA-B35andHLA-A2REFERTO:

a-2allelesfoundin1locus

b-2allelesfoundin2locionchromosome6

ans:b

9)Autoimmunedisease

a-ShowsHLAassociation

b-Runasteadyclinicalcourse

c-Affectbothsexesequally

d-Aretriggeredbydifferentantigenindifferent

individuals

ans:a

questions of the rest of the lectures:


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1)functionsimilartostemcellfactor(SCF)

a)Il-1

b) Il-7c)Il-4

d)Il-2e)Il-5

ans:b/ch12

2)CD4molecules

a).Tyrosinekinase

b)CannotbeexpressedonsurfaceofcytotoxicTcell

c)Includecytoplasmicdomainthathastyrosinekinaseactivity

d)CaninteractwithMHCIontheBcellifCD4moleculesis

expressedon

surfaceofcytotoxicTcell

ans:b/ch12

3)SignaltransductionofBcellinvolvesallexcept

a)Zeta

b)Igalpha


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c)Igbeta

d)Syk

e)Tyrosinekinase

asanswered:a

4)CD19markerisfoundonalloftheseexcept

a)Hematopieticstemcell

b)MatureBcell

c)ProBcell

d)PreBcell

e)ImmatureBcell

ans:a/ch12

4)Hematopoieticstemcellsarepluripotent,whichmeanthat

they:

a.Areantigenspecificcells.

b.Arecapableofdevelopingintoanybloodcells.

c.Arecommittedtoproducecellsofsinglelineage.

d.Arenotselfrenewing.

e.WilldevelopintoTandBlymphocytesofmanydifferent

antigenspecificities.

Ans:b/ch12


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5)Primarylymphoidorgans:

a.AreefficientinexposingTcellstoforeignantigenpresentedondendriticcells.

b.Aretheprimarysiteofantibodysynthesisandrelease.

c.Filterbloodandtrapbloodborneantigens.

d.ProvidethemicroenvironmentformaturationofT&Bcells.

e.Linethemucosalsurfacesofthebodyforefficientantigencontact.

Ans:bch13

6)Lymphocytesareactivatedbyantigeninthe:

a.Bloodstream.

b.Bonemarrow.

c.Liver.

d.Lymphnodes.

e.Skin.

Ans:d/ch13

7)Bcellmaturationoccursin:

a.Thethymus.

b.Thebonemarrow.


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c.Thelymphnodes.

d.Thespleen.

e.Thepeyerspatches.

Ans:b/ch13

8)ApatientisdiagnosedwithBrutonsAgammaglobulinemia.

Whichaspectoftheimmunesystemhistologywould

beyouexcepttobenormal?

a.ConcentrationofsurfaceIgpositivelymphocytesin

peripheralblood.

b.Primaryfactorinlymphnodes.

c.Germinalcentersinlymphnodes.

d.Numberofplasmacellsinbonemarrow.

e.Cellularityofparacorticalareasoflymphnodes.

Ans:e/ch11

9)IgEbindingtomastcellsismediatedby:

a.Fc-dependentcellularhomingmechanisms.

b.Sensitizationofmastcellsandbasophils.

c.FcreceptorsspecificforIgE.

d.ICAMs.

e.Noneoftheabove.


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Ans:c/ch12

10)CD34isamarkerfor:

a.Th1cells

b.Th2cells

c.Gamma-deltaTcellreceptor

d.Bcells

e.Stemcells

Ans:e/ch12

11)Srckinaseactivityisactivatedby:

a.Recruitmenttoareceptor

b.Phosphorylationofatyrosingroupintheactivesite

c.PhosphorylationofITAMgroup

d.PhosphorylationbyCsktocauseaproteinfoldingevent

e.De-phosphorylationofaregulatorytyrosinephosphateinthe

activesite

ans:a/ch11


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12)Lymphocyterecirculationisinvolvedin:

a.Bringingtogetherantigen&rareantigen-specific

lymphocytes.

b.HomingofeffectorTctoavirusinfectionsite.

c.HomingofnaveTcellstoT-cellareasinthelymphnodes.

d.MovementofmatureT&Bcellsfromtheprimaryto

secondarylymphoidorgans.

e.Alloftheabove.Ans:e/ch13

13)Btkgenedefectresultin:

a.X-linkedagammaglobulinemia&ischaracterizedbyBcell

maturationarrestatthepreBcellstage

b.FailureofTcellmaturation.

c.Non-expressionofthisdefectincarrierfemalesduetothe

inactivationofthedefectiveXchromosome.

d.InactivesignaltransductioninducingTcelldevelopment.

e.Alloftheabove.

Ans:a/ch11

14)WhichofthefollowingstatementsaboutCR2(CD21)is/are

correct?


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a.ItpromotesdissociationofC3convertase.

b.ItisexpressedonthesurfaceoffollicularDendriticcells.

c.ItactsasacofactorforFactorI

d.ItisexpressedonthesurfaceofmatureBcells

e.Itcontributestophagocytosisofopsonizedparticlesby

macrophages&neutrophils

ans:d/ch11

15)Allinducesmemoryexcept

a)IL-2

b)CD28andB7

c).onclassIIMHCtoBcell

d)CD40/CD40L

e)CrosslinkingofBcellwithpolymericantigen

ans:e/ch14

16)CD19markerisfoundonalloftheseexcept

a)Hematopieticstemcell

b)MatureBcell

c)ProBcell

d)PreBcell


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ans:a/ch14

17)DuringB-celldevelopmenttherearrangementinIgloci

a)DHtoJH,DJtoVH,VLtoJL

18)ActivationofmatureBcellrequirestwosignals

a)AnantigenandTcellhelp

b)TCRoccupancyandTcellhelp

c)Antigenandcytokines

d)TCRoccupancyandcytokines

Ans:c/ch14

19)Causestolerancerathertheimmunity

a)Insolubleantigen

b)Heterologousantigen

c)Particulateantigen

d)Antigeninjectedsubcutaneously

e)Injectingantigentofetusindevelopment

Ans:e/ch14

20)ProBcellsarecharacterizedby:


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a.Expressionofsurrogateimmunoglobulinlightchain.

b.ExpressionofTCRs.

c.Expressionofc

kit.

d.ExpressionofIgM.

e.Noneoftheabove.

Ans:a

21)Selectthefalse statementregardingthePreBcellreceptor:

a.ItiscomposedbyIgheavychain,surrogatelightchain,and

Ig/Ig

b.ItisexpressedonthePreBcells.

c.EnablesthePre

Bcelltoproceedtomaturation.

d.Itssurrogatechainreplacestheimmunoglobulinlight

chain.

e.ItremainsexpressedatlowlevelsinmatureBcells.

Ans:e

22)BcellsusuallyrequireTcellhelptomatureplasmacells

because:

a.Tcellspresentantigentothem.

b.Mostbcellsinthecirculationneedthymichormones

secretedbytheantigenpresentingcelltomatureto


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plasmacell.

c.Tcellsproducecytokinesthatenhancetheirresponsiveness

toantigen.

d.Alloftheabove.

e.Noneoftheabove.

Ans:c/ch14

23)OnceHchaingeneshavebeenproductivelyrearrangedandexpressedonthepre-Bcellmembrane,thenext

eventtooccurinthecellis:

a.Deathofcellsbindingselfantigen.

b.ExpressionofmembraneIgD.

c.ExpressionofmembraneIgM.

d.Proliferationofthepre-bcells.

e.Somaticrecombinationoflightchaingenes.

Ans:e

24)ThedevelopmentalstepthatcommitsacelltotheB

lineageis:

a.ExpressionofbothmembraneIgMandIgD.

b.Expressionofmembranechain.

c.Expressionofmembranerecombinaseenzyme.


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d.JoiningofaVHgenesegmenttoaDHgenesegment.

e.JoiningofaDHgenesegmenttoaJHgenesegment.

Ans:e

25)Bcelldifferentiationbeginswiththeexpressionof:

a.Membranechain+surrogateLchain.

b.MembraneIgD.

c.MembraneIgM.

d.germlineIgM.

e.RAG-1,RAG-2andTdT.

Ans:a

26)Lymphocyterecirculationisinvolvedin:

a.Bringingtogetherantigen&rareantigen-specific

lymphocytes.

b.HomingofeffectorTctoavirusinfectionsit.

c.HomingofnaveTcellstoT-cellareasinthelymphnodes.

d.MovementofmatureT&Bcellsfromtheprimaryto

secondarylymphoidorgans.

e.Alloftheabove.

Ans:e


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27)Whichofthefollo

wingstatementsabout

Bcellmaturation

(generation)isTRUE?

A.Itrequiresantigen.

B.Pro-BcellshaveIgG

&IgMontheirsurfac

e.

C.Itrequiresstromal

cells&IL-7&occursi

nthelymphnode.

D.ThematureBcells

haveIgM&IgDonits

surface.

Ans:d

28)Whichofthefollo

wingstatementsabout

Bcell

developmentiscorrect?

a.HeavychainVH-DH-JHrearrangementb

eginsatthepre-B

cellstage.

b.Thesurrogatelightchainisexpressedby

immatureBcells.

c.Attheimmaturestage,BcellsexpressI

gM&IgD

d.LightchainVL-JLrearrangementbegins

atthesmallpre-B

cellstage.

e.Thelightchainlociarenotsubjecttoall

elicexclusion

ans:c

QUESTIONS TEAM


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CytokineimportantincontroldisseminationofM.leprosya) TNF-alpha

b) IL-4c) IL8

d)Th2cytokinese)IL-5

answer:d

Differencebetweenprimaryandsecondaryimmuneresponseexcepta) antig-Cneeded


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b. TH1cellshelpinduceIgEproducon

selfpeptides.

Restrictionmeansa)TherecognitionofselfMHCwithantigenbyTcells

answer:a

DevelopmentofCD8+Tcellscouldoccurnormallyintheabsenceof:

a. HLADRgenes.

b. RAG1&RAG2

c.Selfpeptides.d. Tapsainandcalnexin.

e. TAP1&TAP2

PositiveandnegativeselectionofTcells:a. InvolvedifferentMHCmolecules.b. Involvedifferentpeptides.c. Mustinvolvesignalsthatdiffereitherinamountorintype.d. Occurinthesamethymusmicroenvironment.e. ResultinTcellswhichcanneverrecognizeselfantigen.

Whichofthefollowingstatementistrue ofTH1cells:a. TH1cellssecreteIL4.

byBcells.c. TH1 cells smulate Eosinophil

acvaon.d. TH1 cells secretes interferongamma.

Whichofthefollowingstatementistrue ofTH2cells:

a. TH2cellssecreteIL4

&IL10.b. TH2cellssecrete

GMCSF.c. TH2 cells are responsible for cytotoxic T cell

(TC)activation.d. None of the above statementsaretrue.

Whichofthefollowingbest describespositiveselectionofTcells?a. SelectionforcellswhoseTcellreceptorsarecapableofbindingantigenicpeptides.b. SelectionforcellswhoseTcellreceptorsarecapableofbinding

c. Selectionforcellsabletobindimmunoglobulins.


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b. 2

d. SelectionofcellswhoseTcellreceptorsarecapableofbindingselfMHCmolecules.e. SelectionforcellscapableofrejectingforeignMHCmolecules.

WhichofthefollowingbestdescribesnegativeselectionofTcells?a. SelectionagainstcellswhoseTcellreceptorarecapableofbindingselfMHC.b. SelectionagainstcellswhoseTcellreceptorhavinghighaffinityforselfpeptideand/orselfMHCmolecules.c. SelectionagainstcellsthatdonotpresentMHCmolecules.

d. SelectionagainstwhoseTcellreceptorsarenotabletobindantigenicpeptides.e. SelectionagainstcellswhoseMHCmoleculesbindselfpeptideswithhighaffinity.

BcellsusuallyrequireTcellhelptomatureplasmacellsbecause:a. T cells present antigen tothem.

b. Mostbcellsinthecirculationneedthymichormonessecretedbytheantigenpresentingcelltomaturetoplasmacell.

c. T cells produce cytokines that enhance their responsivenesstoantigen.

d. All of theabove.

e. Noneoftheabove.

answer:c

Defenseagainstviralinfectionsrequiresabalancebetweenhumoralandcellmediatedimmunity,asreflectedintherelativeactivitiesofth1andth2cells.Overproductionof

whichcytokineshouldleadtorelativepredominanceofcell-mediatedimmunity?a. IFN- b. IL-4. c. IL-1. d. IL-8. e. IL-2

answer:a

WhichinterleukinisnecessaryfortheswitchtoIgEproduction:a. 1

c. 4d. 5

e. 6

ActivationofThelpercellsbyantigen-presentingcellsrequires:a. CD2b. CD3

c.CD4d. CD28e. Each of the above isrequired.


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AmajormarkerretainedonallperipheralTcellsis:

a. CD3

b. CD4

c. CD8

d. IL-1

e. IL-2

CD34isamarkerfor:

a. Th1cells

b. Th2cells

c. Gamma-delta T cell

receptor

d. Bcells

e. Stemcells

Whichofthefollowingstatementsaboutasecondary(memory)antibodyresponseisFALSE?

a. ThesecondaryresponsecomesfrommemoryBcells.

b. Thesecondaryresponseisfaster.

c. ThesecondaryresponseisprimaryIgM

d. Thesecondaryresponseproducesmoreimmunoglobulinsthantheprimaryresponse.

TuberculoidleprosyischaracterizedbyallEXCEPT:

a. Externisvecell-mediatedresponsetoMycobacteriumleprae

b. HighlevelsofIFNgamma

c. Extermemlyhighlevelsofantibodytothemicrobe

d. Largenumbersofactivatedmacrophagestodestroythemicrobe


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InX-linkedhyperIgMsyndrome:

a. IgG&IgAlevelsinthebloodarehighwhileIgM

levelsarelow

b. TcellscannotengageCD40onBcells

c. Bcellactivation&isotypeswitchingtakeplace

normally

d. Lymphoidtissuelackgerminalcenters

e. Alloftheabove

f. B+D

answer:f

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Immuno First _ Past Years Qs