OOOO ABSTRACTS

Volume 117, Number 2 Abstracts e211

squamous cell carcinoma (SCC) in patients with and withoutcervical metastasis. Study Design: This retrospective,descriptive study used samples from patients initially sub-mitted for surgery related to neck dissection and diagnosedat the National Institute of Cancer in 2001. Sociodemographicand clinicopathologic data were registered. Immunohisto-chemical staining for VEGF-C antibody in patients with andwithout metastasis was performed. Immunoexpression analysiswas qualitative. Results: Study population was composed of41 patientsdmen age 45 to 60 years who smoke and drinkand have tumors of the tongue. VEGF-C immunoexpress-ion was not associated with sociodemographic parameters,however, a statistically significant association (p ¼ 0.015)was found between VEGF-C immunoexpression and metasta-ses to cervical lymph nodes. Conclusion: VEGF-C mustbe studied as a possible biomarker of regional metastasis fororal SCC.

PE-406 - IMMUNOHISTOCHEMICAL ANALYSIS OFCYTOKERATINS ON CALCIFYING CYSTIC ODONTO-GENIC TUMOR. RENATA PILLI JÓIAS, ESTELAKAMINAGAKURA, PATRÍCIA LUCIANA DOMINGOSBATISTA, MARIZE RAQUEL DINIZ DA ROSA, ADRIANOMOTA LOYOLA, PAULO ROGÉRIO BONAN, PAULOROGÉRIO FARIA. INSTITUTO DE CIÊNCIA E TECNO-LOGIA DA UNIVERSIDADE ESTADUAL PAULISTA“JÚLIO DE MESQUITA FILHO”-SJC.

Calcifying cystic odontogenic tumor (CCOT) is an odon-togenic-origin benign cystic lesion characterized by an amelo-blastoma-like epithelium and the presence of ghost cells. Toclarify CCOT’s cytokeratin (Ck) expression pattern, this studyinvestigated Ck6, 13, 14, 18, and 19. Study Design: SevenCCOT cases were classified into four types. The Ck expressionwas evaluated by immunohistochemistry. For analysis, theepithelium lining was divided according to the following re-gions: basal layer, suprabasal layer, and ghost cells compart-ment. Results: Six cases (85.7%) were classified as type 1 andone (14.3%) as 4. All cases were Ck13 and 18 negative. TheCk14 and 19 positivity in all cases reinforced the possibility ofOCCT odontogenic origin, and the Ck6 restricted expression tothe ghost cells may be associated with these cells’ pathogenesis.Conclusion: Ck14 and 19 were positive in all cases and Ck6 inthe ghost cells.

PE-407 - IMMUNOHISTOCHEMICAL ANALYSISOF HYPOXIA INDUCIBLE FACTOR-1ALPHA INPERIODONTAL DISEASE. ROSEANE CARVALHOVASCONCELOS, DENISE HÉLEN IMACULADA PEREIRADE OLIVEIRA, MAIARA DE MORAES, ROSEANA DEALMEIDA FREITAS, ANTÔNIO DE LISBOA LOPESCOSTA, LÉLIA MARIA GUEDES QUEIROZ, BRUNOCÉSAR DE VASCONCELOS GURGEL. UFRN.

This study evaluated the immunohistochemical expression ofhypoxia inducible factor-1alpha (HIF-1a) and correlated it withperiodontal disease. Study Design: The expression pattern and thepercentage of cells immunostained for HIF-1a were evaluated in30 cases of chronic periodontitis, 30 cases of chronic gingivitis,and 15 samples of healthy gingiva. Results: HIF-1a exhibitedpredominantly diffuse nuclear and cytoplasmic staining in in-flammatory and endothelial cells. High expression of HIF-1a

was observed in periodontitis and gingivitis cases compared tohealthy gingiva, but the difference was not statistically significant(p ¼ 0.519). Conclusion: This study suggests that the pathwaysfor HIF-1a transcription are activated in periodontal disease,demonstrating a possible role for this protein in diseaseprogression.

PE-408 - IMMUNOHISTOCHEMICAL CHARACTERIZA-TION OF CELLULAR AND EXTRACELLULAR MATRIXCOMPONENTS OF ORAL MUCOCELES. ADNACONCEIÇÃO BARROS, JAMILE GOMES CONCEIÇÃO,FLÁVIA CALÓ XAVIER DE AQUINO, LUCIANA MARIAPEDREIRA RAMALHO, CLARISSA ARAÚJO SILVAGURGEL, EDUARDO ANTÔNIO GONÇALVES RAMOS,JEAN NUNES DOS SANTOS. UNIVERSIDADE FEDERALDA BAHIA.

Oral mucoceles are caused by damage to the excretoryducts of salivary glands. Materials and Methods: We investi-gated CD34-positive blood vessels, mast cells, macrophages,and matrix metalloproteinases (MMPse1 and -9) using immu-nohistochemical analysis on a series of 32 oral mucoceles.Morphometric and semi-quantitative analysis was done. Results:Mast cells and CD68-positive macrophages as well as MMP-1-,MMP-9-, and CD34-positive blood vessels were seen in allcases. Mast cell accumulation was observed in capsular fibrousbands. An elevated expression of MMP-1 and MMP-9 wasobserved in fibroblasts and inflammatory cells. There were nodifferences between markers (p < 0.05), although a significantassociation was seen between mast cells and MMP-1 (p ¼ 0.03)and between macrophages and MMP-1(p ¼ 0.01). Conclusion:The tissue remodeling seen in oral mucoceles mainly involvedthe migration and interaction of mast cells, macrophages, andMMP-1.

PE-409 - IMMUNOHISTOCHEMICAL COMPARATIVEANALYSIS OF EMMPRIN AND ANGIOGENIC INDEXIN DENTIGEROUS CYSTS, RADICULAR CYSTS, ANDODONTOGENIC KERATOCYSTS. KEILA MARTHAAMORIM BARROSO, CYNTIA HELENA PEREIRA DECARVALHO, LÉLIA MARIA GUEDES QUEIROZ, LÉLIABATISTA DE SOUZA. UNIVERSIDADE FEDERAL DORIO GRANDE DO NORTE.

This study assessed the immunoexpression of extracellularmatrix metalloproteinase inducer (EMMPRIN) and the angio-genic index between odontogenic lesions. Study Design: Thesample was composed of 20 cases each of radicular cysts (RCs),dentigerous cysts (DCs), and odontogenic keratocysts (OKCs).Immunoexpression of EMMPRIN was evaluated in the epithelialcomponent and connective tissue of the lesions. Angiogenic indexwas determined by microvessel count (MVC) using anti-CD105antibody. Results: Expression of EMMPRIN in the epitheliallining did not differ significantly between groups (p > 0.05). Inthe fibrous capsule, immunostaining for EMMPRIN showed asignificant difference for RCs (p < 0.001). MVC revealed asignificant difference between RCs and OKCs (p ¼ 0.018).Positive and moderate correlation was observed between MVCand immunoexpression of EMMPRIN in the fibrous capsule ofRCs (r ¼ 0.654; p ¼ 0.002). Conclusion: The results suggest thatthe expression of EMMPRIN is related to the development of theodontogenic lesions studied.