advanced biotechwww.gnosis-bio.com

Gnosis S.p.A.Via Lavoratori Autobianchi, 120832 Desio (MB) Italy

Gnosis USA Inc.169 N. Main StreetDoylestown, PA 18901 - USA

www.quatrefolic.com

THE OPTIMAL VITAMIN K2

THE OPTIMAL VITAMIN K2

SAFE

NATURAL

VERSATILE

TAILORED FIT

i i k K7Natural vitamin k2 as MK7

®

VitaMK7® contains >99%

ALL-TRANS MENAQUINONE-7,

is >99% PURE (BY HPLC),

is totally Allergen–free and

Soy-free

VitaMK7® derives NATURALLY

from patented biofermentation

process of Bacillus subtilis ssp

natto without any chemical or

solvent

VitaMK7® provides optimal

health benefits clinically proven

Gnosis offers a large range of

vitaMK7® in powder and oil

grades (concentration from

1.000 ppm to 20.000 ppm)

for nutraceuticals and

functional foods

(concentration from

1.000ppm to 20.000ppm)

Tablets

Softgel capsules

Hard capsules

Fortified Food

VitaMK7® VitaMK7® Powder VitaMK7® Soybean Oil VitaMK7® Sunflower Oil K2&Olive®

GNOSIS OFFERS A LARGE RANGE OF VITAMK7® IN POWDER AND OIL GRADES (CONCENTRATION FROM 1.000 PPM TO 20.000 PPM) FOR NUTRACEUTICALS AND FUNCTIONAL FOODS

Natural vitamin k2 as MK7

®

Gnosis features

• Direct from fermentation of B. subtilis natto with no synthetic process

• Solvent Free

• >99% All-trans Menaquinone-7

• >99% Purity Chromatogram

• Gnosis in vitro & in vivo studies

• Kosher approved

• EU GMP manufacturing

• US Patent No. 7,718,407

• EP Patent No. 1,803,820

• JP Patent No. 5,043,425

• No additives and preservatives

• No need for crystal or other forms

• Non-GMO

• No Bacterial Residues

Natural vitamin k2 as MK7

®

THE OPTIMAL VITAMIN K2

Vitamin K2 as MK7 for Bone Health

Overwhelming research shows Vitamin K2 and especially its key isomer, Menaquinone-7, to be

very effective in bone and heart health.

Vitamin K2 is a powerful component in transporting “excess” calcium out of the arteries and blood

and depositing it into our bones.

Vitamin K2 is the essential cofactor for the enzyme γ-carboxylase, which is able to carboxylate a group

of proteins making them able to form calcium-binding groups essential for their biological activity.

CCaCaalccll ififificcatiionn in ccellular tissues iss a sisign oof tissueu

ddaad mmmmaaggeeg , ccelllluululaar aging and a rissk of aasssoco iated

innni juurrryy iinn seevev raal structures and oorganss. DDDefinitely,

tthhhee leeel vevevells oof caalclcium stored in oone’s arrtet rriries are

aannan iinnddn icccaatorr of CaCardiovascularar HHealth.

TTThhhe vvvitaaammin--k deppendent MMatrixx GGla proteeinnn

((MMM( GGPPPPG ) issss knoown as s the mmost poteenent inhibitoorrrrr ofoff

ccccalcccciififificacc tttiiono ppresenttntlyllyy kkkknon wn.

(PPPPPriiccceeec 1999883)

Vitamin K2 as MK7 for Cardiovascular Health

Undercarboxylated ( ) and carboxylated ( )osteocalcin levels during the vitaMK7® treatment

ng /

ml

Weeks

2

4

68

1012

14

0 I II III

Natural vitamin k2 as MK7

®

WITH A PROACTIVE APPROACH FROM PRODUCTCONCEPT DEVELOPMENT THROUGH TO MANUFACTURING AND DISTRIBUTION, GNOSIS OPTIMIZES FOR YOU WHAT NATURE OFFERS

• VitaMK7® derives NATURALLY from patented biofermentation process of Bacillus subtilis ssp natto

cultures. The identity of Gnosis’ proprietary strain has been genetically characterized by the most

advanced tchniques

• No other type of Bacillus are used by Gnosis (i.e. Bacillus licheniformis used in other K2 derivates)

• VitaMK7® is totally NATURAL and the unique production process is patented in Europe, USA and Japan

and garantees:

VitaMK7® is the most advanced Vitamin K2 Menaquinone-7 product in the market

No chemicals

No solvents

No additives and preservatives

No synthetic process

p g g g p

Vitamin K2 Uptake

Carboxylated Osteocalcin (c-OC)

UnderCarboxylated Osteocalcin (c-OC)

Volts

Minutes10

0

20

40

60

80Retention Time

23.353

21.613

25.840

15 20 25 30 35 40

Isomeric purity identification of Menaquinone-7 by HPLC analysis

VitaMK7® is the All-trans Vitamin K2 Menaquinone-7 productin the market

Contrary to what is frequenntly beleliievedd,d, thee

vitaMK7® is not soy-based d ffermentteed.

In order to guarantee the highest qquality y profile ooof

vitaMK7®, Gnosis testes the potennntial level of f ssosoy

protein on the starting material usses d to

manufacture the ingredient.

VitaMK7® is Allergen-freeand Soy-Free

10,80,60,40,20

CVD

Low intakeHigh intake

SevereCalcific.Vessels

Deathfrom CVD

All CausesMortality

ViVittammimiiminnn K2K2 ccan exiists inn bothh ccisis– aaananannd trananns-s form.

Fromom thehe literatturee iititit iiiss kknoowwnn thaat ccccisisi -anaaalologs of

vivitataataammim n K aare biologggiicical inaactctive.

(K(K(K(((( nnannaueueueerrr rr 1919197775757 ))

Contrary to sysysysysysyntntnnn hheetiticc prprododducuctitit ono ,, GnGnoosiss’ pateenntn

grgrgrgrgrananana tet d biofermentntntatatatiioionn prprproccocesese s s can prprovvovididde thheee

highhghessese tttt cocococontent of all–trans strtrtrucucu tuuturee MK-K 7 77 ininnin

vvivitat MKMKK77®..

Effect of vitamin K2 as MK7 on osteocalcin carboxylation in healthy volunteers

ddrdrdramamamatatatiicicalallylyy rredededucucuceseseses ttttthehehehehe rrrrisissisisskkkkk StStududieiess hahaveve ppprorororoveveveveennnn n thtthththhatataa ViVitatamiminn n K2K2K2K2 MMMMK-K-KK-K-7777

mortatalilitytyy wwwhehehennn tatatakekekekekennnn ddadaadadaailililililyyyyyyfof ccarararaardidid ovovasascuculalarr dididiseseseasasaseseses ((((CVCVCVD)D)D)D) aaaandndndnd eeeearlllyyy(Th(The Rotterdam Study dy – 2– 200004) )(Thh Re Rotterdam Study 200404))

Vitamin K is essential for

the proper formation

and full activation of the

Gla proteins. The Gla

protein osteocalcin,

when fully carboxylated

by vitamin K, allows for

the binding of calcium

to the bone matrix.

V

Ca++

Ca++

Ca++

Soy-free

Allergen-free

100% non-GMO product

Gluten-free

Lactose-free

Toxins andpesticides-free

Maanyny stutudies have shown that tthe serum osteocacalcin

level isis a ssene sitive marker of boone turnover thaatt

rer flects spspeccifiificac lly bone formaation.

Ann iin vivvivov studyy:: ththe overall acctiti ivitty off cells operating

ininn bbonne e fofoormrmr ation in hhheaeaealtltlthyhyhy vvvooollolunununteteererss was

signifiifificaccantntlyly affffececteted by treatmeent already after 1 weeeekk kk

ofofofofof tttttrerereatmentntt ooooff f vivitataMKMK77®®®. ThThThee raraaatititio ofof ccOCOC/u/u/uOCOCO which

inini crc eaaseseeseddd bybyby 42%.