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1 essenbioscience.com/IncuCyte
IncuCyte™ Reporter Gene AssayReal-time measurement of reporter gene expression
Real-time visualization and analysis enable interrogation of cell morphology changes with signalling pathway activation.
Measure signaling pathway activation and activity of any promoter driven recombinant GFP/RFP reporter gene expression in real time.
Save cost and time – no terminal reaction substrate and defining end points a priori.
Schematic representation of the NFκB reporter gene concept. Cells are transfected or infected with a vector containing the NF-κB transcriptional response element just upstream of the GFP reporter gene. Cells are then treated with TNFα which activates the TNFα receptor followed by degradation of IκBα, and translocation of the NFκB transcription factor subunits to the nucleus where they participate in driving the expression of the GFP transgene. Green fluorescing cells can be kinetically imaged using the IncuCyte® ZOOM Instrument and the number of fluorescing cells can be quantified.
HEK293 cells were transiently transfected with an NFkB-GFP reporter gene construct, serum starved, and treated with the indicated amounts of TNFα. Images were acquired every 2 hours. Summed intensity is reported as a marker for NFkB promoter activity.
2 essenbioscience.com/IncuCyte
Essen BioScience, 300 West Morgan Road, Ann Arbor, Michigan, USA 48108 © 2016 Essen BioScience. All rights reserved. All trademarks are the property of Essen BioScience unless otherwise specified.
3D-Spheroids Angiogenesis Apoptosis Cell Culture QC
Cytotoxicity Neurite Dynamics - Label-Free
Neuronal Co-Culture - Fluorescence
Proliferation - Cell Count
Proliferation - Confluence
Reporter Gene
Stem Cell Monitoring & Reprogramming
Transfection Efficiency
Immune Cell Killing, Clustering & Proliferation
Scratch Wound Migration & Invasion
Dilution Cloning
Phagocytosis
ChemotaxisMigration & Invasion
Learn more at essenbioscience.com/applications.
Or contact us at [email protected].
Key applications using the IncuCyte® ZOOM Live-Cell Analysis System
8000-0125-D00
IncuCyte™ Reporter Gene AssayReal-time measurement of reporter gene expression