Index to subjects

Volume 1 Number 4 August 1989

Acetylcholine, reuptake, 737 in synaptic vesicles, 655

Acetylcholine receptor, axial section, 697 Acetylcholine receptor channels, nerve and muscle, 754 N-acetylgalactosamine, 0-glycosylation, 649 N-acetylglucosamine-l-phosphotransferase, action on cathepsin

D, 637 Actin-binding proteins, 657 Acyclovir, entry into human erythrocytes, 725

Acyl-CoA oxidase, peroxisomal pathway, of fatty acid oxidation, 630 topogenesis, 631-632

translocation, energy requirement, 631 Adaptor proteins, clathrin-coated vesicles, 618

Adenylate cyclase, stimulation and activation, 669-670 a-factor,

prepro-, in yeast, 636 translocation, mammal ER vesicles, 636

Amines, biogenic, uptake, 736 Amino acid transporters, 725

Angler&h, prosomatostatin I and II, 650 Ankyrin, MDCK cells, 664, 667 Annexins, function, 643

Antigen-antibody complex transport, 641 Antipotters,

anion exchange, 729-730 Na+/Ca*+ exchange, 731-732

Na+/H+ exchange, 730-731 others, 732

AOx, see Acyl-CoA oxidase Aplysia,

hormone packaging, 650

neuropeptides, 656 ASClasc, amino acid transporters, 725 Ascites, isoleucine/sodium system, 740 Assembly of proteins, endoplasmic reticulum, 635-637 Assembly proteins and adaptors, clathrin-coated vesicles,

685-688 ATPase,

F-, V- and P-types, 746 proton-translocating, Hansenula polymorpha, 631

Auditory transduction, ion channels, 758-759

Bacterial membranes, binding protein-dependent transport systems, 702 exported proteins, 703-705 membrane transport, 701 membrane-associated proteins, 703 membrane-bound systems, 703

phosphotransferase systems, 702-703 Bacterial sugar transporters, 704 Bacterial transporters, evolutionarily related, 703-704

Band 3 protein, capnophorin, 729 p-adrenoceptor,

desensitization, 670-671 functional domains, 669-670 truncation, 670

Bilirubin, transport, 714 Biosynthetic pathway, intracellular membrane fusion, 641 Biosynthetic protein transport,

exit from ER, 648 retention of ER-resident proteins, 648-649 role of cis-Golgi system, 648-649 secretory pathway, 648 transport to Golgi complex, 648

BiP see Heavy chain-binding protein Brefeldin A, reponse of cis and medial Golgi, 637 Bumetanide, inhibitory effect on cotransporters, 742

Calcium channels, intracellular messengers, 756-757 ion concentration changes, 767

Calcium ions, role in intracellular fusion, 643 Calmodulin-inhibited calcium-release channels, 760 Calpectin, function, 643

. Candida bordmrr, methanol oxidase, import inhibition, 631 Candida maltosa, transfection of topogenic information,

C. tropicalis, 632 Candida tropicalis, targeting AOx to peroxisomes, 632 Capnophorin, band 3 protein, 729 Carbonylcyanide-m-chlorophenylhydrazone, protonophore, 631 Catalase, see Peroxisomes

Cathepsin D, KDEL-sequence fusion, 637 CCCP, see Carbonylcyanide-m-chlorophenylhydrazone, 631 Cell surface receptors, see Plasma membrane receptors Chloroplast proteins,

protein import, 706-707 stromal protein assembly, 707-708 targeting and assembly, 706-709 targeting to chloroplast compartments, 708 . thylakoid membrane insertion, 709 transit peptides, 706

CHO cells, peroxisome assembly defects, 634 vesicles and recycling, 678

Cholera toxin, B subunits, 697 Cholesterol, binding sites, 695 Chromaffin granules,

model membrane systems, 772 transport, 656

Chromobindins, function, 643 Chromogranins, structure, 651 Clathrin,

cofactors, 685686 HA1 and HA2, 685 heavy chain, 684-685

@ Current Science Ltd ISSN 0!355+674 875

876 Index to subjects

prevention of ‘exocytosis-like’ fusion, 641 * structure, 684

Clathrin-coated vesicles,

adaptor proteins, 618 assembly proteins and adaptors, 685-688 sorting signals, 618 structure, 684-685

Clathrin-independent endocytosis, 677

Clathrin-mediated endocytosis, 675-677, 675-677 Colcemid, microtubule disruption, 666 ‘Conservative sorting’, mitochondrial proteins, 627 Constitutive receptors, internalization signal, 675 Constitutive secretory pathway, Colgi complex, 650 Cotransport system,

chemical labeling and the isolation of associated polypeptides, 742

kinetics, 739-742 molecular biology, 742-743 physiological role, 742

Crithidia fasciculata, glycosomes, phosphoglycerate kinase, 633 CV-1, monkey kidney cells, peroxisomes, 631 Cytochalasin B-binding inhibition of glucose transport, 721-722,

724 Cytochrome c,

heme lyase, mutants, 627 import pathway, 627 precursor protein, 627

Diuretics, inhibitory effect on cotransporters, 742 Domains, functional activity, identification, 669-673 Down-regulation, p-adrenoceptor, 670 Drosophila, white eye locus, and bacterial membranes, 702 Drug-induced hepatitis, 714

Endocytosis, acidic pH, 677 clathrin-independent, 677 clathrin-mediated, 675-677 dissection of pathway using cell-free sytems, 680-681 internalization, 677 intracellular membrane fusion, 639641 and intracellular transport, 617 lysosomal pathway, 678-679 pH regulatory elements, 677-678 rate, and mutation, tyrosine residues, 618 sorting and recycling 678 two models, 679680

Endoplasmic reticulum (ER), assembly of proteins, 635-637 events in the membrane, 636 hepatic, molecular pathologies, 712-716 molecular pathologies, 712-716 nascent chain-ribosome complex, 636 retention in the ER, 636-637 targeting to ER membrane, 635-636

Endosomes, tubulo-vesicular structure, 678

types, early/late, 639 5-enoylpyruvylshikimate-3-phosphate synthase (EPSP), 707 Epidermal growth factor receptor, tyrosine kinase activity, 672 Epithelial cell membranes, pores, 773

Epithelial cell polarity, domains, 660 localization of membrane proteins, table, 661-665 mechanisms, intracellular sorting of proteins, 665-667 stages in generation, 660661

EPSP, see 5-enoylpyruvylshikimate-3-phosphate synthase Erythrocytes,

anion exchanger, prototype antiporter, 729 Band 3 exchanger, 663 glucose transporters, 721-724 membrane permeability, 772-773 model membrane systems, 772-773

Escherichia co/i,

4.5 S RNA, SRP, 636 binding protein-dependent transport systems, 702

groEL, ‘heat-shock protein’, 627, 708 lactose/proton system, 740 membrane transport proteins, 723 Na+/H+ exchange, cloning, 731 signal recognition particle, 636

N-ethylmaleimide, inhibition of transport in cell-free systems, 641 and transport across mitochondrial membrane, 636

N-ethylmaleimide-sensitive factor, composition, 641 identification, 680681 induction of membrane fusion, 641

Fibroblasts, Colgi apparatus, 616 Firefly, luciferase, packaging into peroxisomes of kidney, 631 Fluorescein isothiocyanate, effect on cotransporters, 742 Furosemide, inhibitory effect on cotransporters, 742

G-protein, interaction with B-adrenoceptor, 669 intracellular second messengers, 758

CABA, see y-aminobutyric acid y-aminobutyric acid (CABA),

glycine receptor channels, 754, 754 neurotransmitter transport, 736, 737

General insertion protein, intramitochondrial sorting 627 release of signal recognition particle, 636

Gip, see General insertion protein Glucose transporters,

and insulin, 724 kinetics, 721 mammalian, ‘erythroid/brain’ carrier, 723 ‘insulin-regulatable’ carrier, 723 ‘liver’ carrier, 723 molecular characterization, 722-724

Clucuronides, and protein acylation, 715 Glutamate receptor channels, 754 Clutathione-S-transferases, function, 714 Clycine receptor channels, 754 Glycogen storage disease, 712-714 Glycoproteins, see also Membrane glycoproteins; Viral

glycoproteins Glycosomes, protein topogenesis, 633 Golgi apparatus, fibroblasts, 616 Colgi complex,

Index to subiects 877

biosynthetic protein transport, 648-650

constitutive secretory pathway, 650 exit:lysosomal proteins, 651-652 kinetics and regulation, 649-650 mannose-6-phosphate receptor cycling, 652 prelysosome/late endosome, 651-652 regulated secretory pathway, 650-651 secretory epithelial cells, 616

simple epithelial cells, 616 trans Colgi network CTGN),

intracellular transport, 639 M-6-P-containing proteins, sorting, 618 pathways of protein delivery, 620

Colgi region, 25 kD-proteins, 619

tram Golgi sorting, sorting signals, 618 cis Golgi system,

analogy with TGN, 649 biosynthetic protein transport, 648-649

post-translational modification, 618 CRP78, homology with heavy chain-binding protein, 636 CST, see Clutathione-5-transferases CTP-binding proteins,

biosynthetic protein transport, 649 classification, 642 in intracellular fusion, 642 role in intracellular fusion, 643

targeting label, carrier vesicles, 642-643 Guanine nucleotide regulatory protein, see G-protein Gustatory transduction, 759-760

Haemagglutinin, targeting to correct surface, 619

Haemophilus, binding protein-dependent transport systems, 702 Halobacteria, signal recognition particle, 636 Hansenula polymorpha,

over-production of alcohol, 632 proton-translocating ATPase, 631

Harmaline, inhibitory effect on cotransporters, 742 Heat-shock protein Hsp60,

‘chaperonin’, 627 homologies, 627 yeast mutants, 627

Heat-shock-related protein, GRP78, homology with heavy chain-binding protein, 636

Heavy chain-binding protein, associated with haemagglutinin, 636-637 retention in ER, 637

role, 636-637 Hepatic endoplasmic reticulum, molecular pathologies, 712-716 Hepatitis, drug-induced, mechanism, 714 Hepatocytes,

plasma-membrane receptor protein, 714 post-endocytotic sorting, 619

xenobiotics and endogenous compounds, 714-716 Histidinol, selection of mutants, 636 Histidinol dehydrogenase, HIS4 gene, 636 Hormone packaging Aplysia, 650 Hormone-binding protein (HBP25s), 651

Hsp60, see Heat-shock protein Hydrophobic plot, 719 Hydrophobic proteins, synthesis within mitochondria, 627

lmmunoglobulin receptor, polymeric fplg), 618 Immunoisolation, of organelles, 652 Influenza virus haemagglutinin,

membrane fusion proteins, 644 tyrosine mutation, 618

lnositol polyphosphates, intracellular second messengers, 757 Inositol-glycan second messenger, 672 Insulin, proinsulin mutant, 650

Insulin receptor, structure and activity, 671-672 Insulin-like growth factor, (mannose-6-phosphate receptor),

687-688 Intracellular membrane fusion,

biosynthetic pathway, 641 endocytic pathway, 639-641 identification of fusion machinery, 641 induced by proteins, 644-645

molecular mechanisms, 643 Intracellular transport, and endocytosis, 617 Ion channels of nerve and muscle,

calmodulin inhibition, 760 directly modulated, 757-758 excitation-contraction coupling 757 ligand-gated channels, 753-754 modulation, 760

nicotinic ACh receptor channels, 754 phosphorylation, 760 sensory transduction, 758-760 voltage-gated channels, 765-757

Ion pumps, introduction, 746 P-type ATPases, 747-750 structure, 747-748

Ion-coupled neurotransmitter transport, 735-737

KDEL C-terminal signal, added to cathepsin D, 637, 648 recycling, 618 recycling to the ER, 648 RER retention signal, 617 specific receptor, 637

Langmuir-Blodgett film, lipids, 693 Lanthanides, renal brush-border system, 742 Lateral diffusion, and membrane structure, 692-693 LHCP, see Light-harvesting chlorophyll-binding protein Ligand-gated channels, nerve and muscle, 753-754 ’ Ligandin, see Glutathione-5-transferase Light-harvesting chlorophyll-binding protein, insertion into

thylakoid, 709 Lipid-protein interactions, specific/non-specific, 694-697 Lipids, sensors of membrane surface potential, 692 LMP, see Lysosomal membrane glycoproteins Low density lipoprotein receptor, clathrin-mediated

endocytosis, 675-677 Luciferase, firefly, packaging into peroxisomes of kidney, 631 Lysosomal enzymes, N-linked oligosaccharides, M6P receptor,

679 Lysosomal membrane glycoproteins (LMP), 678-679 Lysosomal pathway, endocytosis, 678-679 Lysosomal proteins, Golgi complex, 651-652 Lysosomes,

878 Index to subjects

biogenesis, 679 . yeast, 652

Mannose6-phosphate, protein receptor, internal signal, 615 Mannose-gphosphate receptor,

and clathrin HAl, 687 cycling, 652 (insulin-like growth factor), 687-688 large cation-independent form, 618 lysosomal enzymes, 679

Mannose-6-phosphafe signal, cis-Colgi, 618 MDCK cells,

membrane cytoskeleton, 663-664 sorting signals, polarized cells, 619

Melittin, 696 Membrane dynamics,

membrane surfaces, 691-692 zwitterionic groups, 692

Membrane fusion, see Intracellular membrane fusion Membrane fusion proteins, 644 Membrane glycoproteins, viral, mimicking, 615 Membrane permeability, overview, 719 Membrane potentials,

ion concentration changes, 767 role of ion currents, 766-767 role in neuronal cells, 765-769

Membrane proteins, folding and oligomerization, 648 Membrane structure,

glycoproteins, 693-694 hexagonal phases, 695 and lateral diffusion, 692-693 protein structure and localization, 697-698 protein-lipid interactions, 694-697 surfaces, 691-692

Membrane transport, methodological advances, 652 Membrane transport protein, membrane-spanning helices, 774 Membranes,

model membrane systems, 771-772 see also Bacterial membranes

Methanol oxidase, Candida boidinii, import inhibition, 631 Micellar intermediate, inverted, 645 Microtubule disruption, drugs, 666 Mitochondrial membrane, porins, 772 Mitochondrial proteins,

bypass transport, 625 ‘conservative sorting’, 627 precursors, ATP requirement, 626 intramitochonrial sorting 627-628 processing and folding, 626-627 specific recognition, 624-626 targeting signals, 624-625 transport and unfolding 626 surface receptors, 625

Mono-oxygenases, intravesicular, 658 Monoclonal anti-trinitrophenol antibodies, 693 Mouse mammary tumour virus glycoprotein gp70, biosynthetic

protein transport, 650 Mycoplasma, binding protein-dependent transport systems, 702

Na+/Ca*+ exchange, antiporters, 731-732

Na+/H+ exchange, antiporters, 730-731 Nascent chain-ribosome complex, endoplasmic reticulum, 636 Neuroendocrine secretory granules, three types, 650-651 Neurospora, potassium/proton transporter, 739, 741 Neurotransmitter transport,

ion-coupled, 735-737 reuptake systems, 736 storage, 736-737 structure and function of transporters, 737 in synaptic transmission, 735-736

Neurotransmitters, see Synaptic vesicles, cell biology Nicotinic ACh receptor channels, ion channels of nerve and

muscle, 754 Nitrobenzylthioinosine, inhibition of facilitated diffusion, 724 NMDA receptors,

Alzheimer’s disease, 766 Huntington’s disease, 766

NMDA/non-NMDA receptor channels, 759 Nocodozole, microtubule disruption, 666 NSF, Golgi complex, 649 Nucleoside and nucleobase transport, 724-725 Nucleotides, intracellular second messengers, 758

0-glycosylation, N-acetylgalactosamine, 649 Olfactory transduction, 759 Oligomerization of membrane proteins, 648 Ouabain, in ATP-dependent acidification, 677

P-type ATPases, 746 Para-aminohippurate transporter, renal brush-border vesicles,

732 Parathyroid hormone, membrane transport, 742 Peptidergic secretory vesicles, specific proteins, 658 Perforated cells,

constitutive secretory pathway, 650 membrane transport studies, 652, 648

Peroxisome biogenesis, deficiency in Zellweger syndrome, 633 machinery for translating proteins, 630-631 mutations, in import machinery, 633 topogenic information, 631-633

Peroxisomes, transfection of topogenic information, 632-633

Phloridzin-sensitive glucose uptake, 742-743 Phosphoenolpyruvic acid (PEP), phosphotransferase systems,

702-703 Phosphoglycerate kinase, glycosomal/cytosolic, 633 Phospholipid vesicles, fusion, 643 Phosphotransferase systems, bacterial membranes, 702-703 Photobleaching, fluorescence recovery, 692-693 Photoreactive crosslinker, insertion into signal sequence, 636 Plasma membrane, basal and lateral domains, 660, 662-663 Plasma membrane receptors,

autophosphorylation domains, 673 g-adrenoceptor, desensitization, 670-671 functional domains, 669 identifying active domains, 669-673

insulin receptor, 671-673 protein, hepatocytes, 714

Plasmalogens, 633 Polarized cells, sorting signals, 619-621

Index to subjects 879

Polarized epithelial cells, see Epithelial cell polarity Polymeric fplg) immunoglobulin receptor,

clathrin-coated vesicles, 618 independent sorting signals, 621

targeting to correct surface, 619-621 Pores,

epithelial cell membranes, 773 model membrane systems, 771-772

permeability, 771-774 red blood cell membranes, 772-773 subcellular organelles, 772

Porins, mitochondrial membrane, 772

Potassium channels, membrane potentials, 768 voltage-gated ion channels, 756

Potassium/proton transporter, 739 Precursor protein, unfolding for membrane translocation, 626 Processing enhancing protein (PEP), inner mitochondrial

membrane, 626 Proinsulin, mutant, increased secretion, 650 Prosomatostatin I and II, anglerfish, 650 Protein,

secretion, constitutive/regulated pathways, 618-619 sorting signals, see Sorting signal in synaptic vesicles, 657-658

Protein acylation, and glucuronides, 715 Protein llla and Illb, see Synapsins Protein transport, into mitochondria, 624-628

Protein-lipid interactions, specific/non-specific, 694-697 Pyridoxal phosphate, transport inhibitor, 729

Receptors, see Plasma membrane receptors Recycling pathways, evidence, 678 Renal brush-border system,

cotransport system, 740

lanthanides, 742 Renal brush-border vesicles, para-aminohippurate transporter,

732

Ribonucleoprotein, signal recognition particle, 636 Ribulose 1,5-bisphosphate carboxylase,

‘chaperonin’, 627 and Escherichia co/i groEL gene, 708

protein assembly, 708 small subunit precursor, 706

Rotavirus, membrane-associated protein VP7, 649 Rough endoplasmic reticulum, soluble lumen proteins, 617 Rubisco, see Ribulose 1, 5-bisphosphate carboxylase

Saccharomyces carlsbefgensis, glycine/proton symport, 740 Salmonella, binding protein-dependent transport systems, 702 Schizosaccharomyces, see also Yeast Schizosaccharomyces pombe, signal recognition particle, 636 Second messengers, modulating ion channels, 757 Secretogranins, structure, 651

Secretory pathway, biosynthetic protein transport, 648-652 Secretory proteins,

aggregation-mediated sorting 651 possible sorting mechanims, 651

Secretory vesicles, peptidergic, 658 Semliki Forest virus glycoprotein, transport, calcium

requirement, 643 Sendai virus glycoprotein, lateral diffusion, 693 Sensory transduction, ion channels of nerve and muscle,

758-760 Serine protein kinase, 672 SFV, see Semliki Forest virus Shiga toxin receptor, HeLa cells, 676 Signal peptide route, exported proteins, 703-704 Signal recognition particle,

binding, 615 ribonucleoprotein, 636

Signal sequences, photoreactive crosslinker, 636 proteins entering ER, 635

Site-directed mutagenesis, 719 Sorting signals,

for clathrin-coated vesicles, 618 identification, 617 in polarized cells, 619-621 trans Golgi sorting 618

Spectrin, MDCK cells, 664, 667 Spike glycoprotein, vesicular stomatitis virus WV-G), 640 SRP, see Signal recognition particle Streptococcus, binding protein-dependent transport systems,

702 Sugar transporters, bacteria, yeast and man, 704 Symporters, see Cotransport system Synapsins, in synaptic vesicles, 657 Synaptic currents, excitatory/inhibitory, 766-767 Synaptic transmission, neurotransmitter transport 735-736 Synaptic vesicles,

cell biology, 655-656 proteins, 657-658 transport functions, 656-657

Synaptobrevin/VAMP-I, -homologue in Drosophila, 658 synaptic vesicles, 657-658

Synaptophysin, in synaptic vesicles, 657 Synaptosomes, 736 Synexin, function, 643

Tetraethylammonium transport, 732

TGN, see trans Golgi network Thylakoid, see Chloroplast proteins Tienilic acid hydroxylation, drug-induced hepatitis, 714 Tight junctions, breakdown, 666 Torpedo, synaptobrevin/VAMP-1, molecular cloning, 657-658 Transit peptides, chloroplast proteins, 706 Transporters,

overview, 719 see Glucose transporters

Transverse tubules (t-tubules), 757 Trypanosoma brucei, glycosomes, protein topogenesis, 633 Trypsin cleavage, glucose transporters, 722 Tubules, intermediates in recycling, 678 Tubulovesicular network, 640

Tyrosine domains 1, 2 and, 3, 673 Tyrosine residues, mutation, and rate of endocytosis, 618 Tyrosyl kinase,

autophosphorylation domains, 673 signal transduction, 672-673

880 Index to kbjects

Uniport carriers for metabolites, 721-726

Vesamicoi receptor, 736-737 Vesicles, possible tracks through cytoskeleton, 666 Vesicular carriers, 639640 Vesicular fusion,

GTP-binding proteins as targeting labels, 642-643 in intracellular transport, 639

Vesicular stomatitis&us, spike glycoprotein, 640

Vesicular transport, assays, principles, 615 Vesiculotubular compartment, 649 Viral glycoproteins, use as probes for membrane traffic, 615 Visual transduction, 759 Voltage-gated channels, nerve and muscle, 765-757

Yarrowia /ipo/ytica, signal recognition particle, 636 Yeast,

biogenesis of lysosomes, 652

glucose transporters, 723

glycine/proton symport, 740 HIS4 gene, 636

peroxisomes, pH, 631 secl2 and sec23 genes, 648

sec78 gene, N-ethylmaleimide-sensitive factor, 641 signal recognition particle, 636

stimulation of translocation, 636 stimulatory factors Ssalp and Ssa2p, 636-637

sugar transporters, 704 YTPI gene product, effect on vesicular transport, 643

Yeast mutants, deficiencies in ‘heat-shock protein’, 627

deficiencies of maturation, mitochondrial proteins, 626

deficiencies in PEP and processing peptidase, 626

Zellweger syndrome, 633

Zwitterionic groups, membrane dynamics, 692