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Advantages of Inertsil ODS-EP
● The selectivity is completely different from those of conventional
columns such as ODS column due to its specific polar group in the
stationary phase.
● Durability can be obtained by the high density bonding.
● Superior peak shapes of both acids and bases can be obtained because of
neutral bonded phase and low residual silanol groups.
● Silica gel of high-purity is used as the support material, which
minimizes effects of any remaining metal ions.
● Specifications of Inertsil ODS-EP
Support Silica Gel Chemical Modification
Partical
diameterShape
Specific
surface area
Pore
diameterPurity Bonded group
ODS Carbon
content
Inertsil ODS-EP 5μm Spherical 450m2 /g 100Å 99.999Embededpolar
Octadecyl group9 %
(1) Naphthalene sample
~ for measurement retention and peak shape ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : Water =65 : 35
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 254 nm
Inertsil ODS-EP
Peak identification
1. Acetophenone
2. Benzene
3. Toluene
4. Naphthalene
0 2 4 6 8
Time (min)
1
2
3
4
0 2 4 6
Time (min)
Symmetry Shield RP18
0 2 4 6
Time (min)
Xterra RP18
0 2 4 6
Time (min)
Discovery RP Amide C16
0 2 4 6
Time (min)
Zorbax Bonus-RP
0 2 4 6
Time (min)
Hypersil HyPURITY Advance
Inertsil ODS-EP
Comparison of retention between Inertsil ODS-EP and commercial
embedded columns
0 2 4 6
Time (min)
0 2 4 6
Time (min)
Aqua C18(polar endcapping)
0 2 4 6 8
Time (min)
Inertsil ODS-3
Inertsil ODS-EP
0 2 4 6
Time (min)
Inertsil C8-3
0 2 4 6
Time (min)
Inertsil Ph-3
Comparison of retention between Inertsil ODS-EP and other type
commercial columns
0 2 4 6
Time (min)
(2) Selectivity test sample ~ for measurement selectivity ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : Water = 70 : 30
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 265 nm
Inertsil ODS-EP
Peak identification
1. Ethylbenzene
2. Naphthalene
3. n-Propylbenzene
4. n-Butylbenzene
5. Anthracene
(CH2)3CH3
C2H5
(CH2)2CH3
0 2 4 6 8 10
Time (min)
1
2
34
5
0 2 4 6 8 10
Time (min)
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-EP
Comparison of selectivity between Inertsil ODS-EP and commercial
embedded columns
0 2 4 6 8
Time (min)
n-Butylbenzene
Anthracene
0 2 4 6 8
Time (min)
n-Butylbenzenen-Butylbenzene
n-Butylbenzene
Anthracene
Anthracene
0 2 4 6 8
Time (min)
n-Butylbenzene
+ Anthracene
0 2 4 6 8
Time (min)
n-Butylbenzene
+ Anthracene
0 2 4 6 8
Time (min)
Anthracene
Aqua C18(polar endcapping)
Inertsil ODS-3
Inertsil ODS-EP
Inertsil C8-3
Inertsil Ph-3
Comparison of selectivity between Inertsil ODS-EP and other type
commercial columns
n-Butylbenzene
Anthracene
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8
Time (min)
0 2 4 6 8
Time (min)
n-Butylbenzenen-Butylbenzene
n-Butylbenzene
n-Butylbenzene
Anthracene
Anthracene
Anthracene
Anthracene
0 2 4 6 8 10
Time (min)
The selectivity of Inertsil ODS-EP is completely different from
those of conventional columns such as ODS column due to its
specific polar group in the bonded phase.
Comparison of retention and selectivity between Inertsil ODS-EP and commercial embedded columns
0.00
1.00
2.00
3.00
4.00
5.00
6.00
7.00
8.00
9.00
10.00
0.80 1.00 1.20 1.40
α(Anthracene/Butylbenzene)
k' (n-
But
ylbe
nzen
e)
Inertsil ODS-EP
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-3
Inertsil C8-3
Inertsil Ph-3
Aqua C18
Inertsil ODS-EP
0 10 20 30
Time (min)
(3) Tanaka-test sample
~ for measurement selectivity, hydrogen bonding capacity
and hydrophobicity ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : Water = 80 : 20
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 254 nm
Inertsil ODS-EP
Peak identification
1. Uracil
2. Caffeine
3. Phenol
4. n-Butylbenzene
5. n-Amylbenzene
6. o-Terphenyl
7. Triphenylene
1
2
3
45
67
0 10 20 30
Time (min)
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-EP
0 10 20
Time (min)
0 2 4 6 8 10
Time (min)
0 2 4 6 8 10
Time (min)
0 2 4 6 8 10
Time (min)
0 2 4 6 8 10
Time (min)
1
2
3
4 56 7
1
2
34
56
7
1
2
34 56
7
1
2
34 56
7
1+2
345+6
7
1
23
456
7
Comparison of selectivity between Inertsil ODS-EP and commercial
embedded columns
0 10 20 30
Time (min)
Aqua C18(polar endcapping)
Inertsil ODS-3
Inertsil ODS-EP
Inertsil C8-3
Inertsil Ph-3
Comparison of selectivity between Inertsil ODS-EP and other type
commercial columns
0 10 20
Time (min)
0 2 4 6 8 10 12 14
Time (min)
0 2 4 6 8 10 12 14
Time (min)
0 2 4 6 8 10
Time (min)
1
2
34 5
6 7
1
2
3 4 56
7
1
2
3
4
5+67
1
2
3 4 56
7
1
23
45
6+7
0.00
0.10
0.20
0.30
0.40
0.50
Inertsil ODS-EP Symmetry Shield
RP18
Xterra RP18 Discovery RP
Amide C16
Zorbax Bonus-RP Hypersil HyPURITY
Advance
α(caffeine/ph
enol)
Hydrogen bonding capacity
Hydrophobicity
Steric selectivity
Characterization of Inertsil ODS-EP and commercial embedded columns by Tanaka test
(1) Caffeine shows no retention
(1)
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : Water = 80 : 20
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 254 nm
Phenol
O CH 3
CH3 N
N
N
O N
CH3
( CH 2 ) 4 CH 3 ( CH 2 ) 3CH 3
OH
Triphenylene
n-Amylbenzene
o-Terphenyl
n-Butylbenzene
Caffeine
0.00
0.50
1.00
1.50
Inertsil ODS-EP Symmetry Shield
RP18
Xterra RP18 Discovery RP
Amide C16
Zorbax Bonus-RP Hypersil
HyPURITY
Advance
α(a
mylbe
nzen
e/bu
tylben
zene
)
0.00
0.50
1.00
1.50
2.00
2.50
3.00
Inertsil ODS-EP Symmetry Shield
RP18
Xterra RP18 Discovery RP
Amide C16
Zorbax Bonus-RP Hypersil
HyPURITY
Advance
α(trip
heny
lene
/o-terph
enyl)
(4) Pyridine-Phenol sample
~ for measurement of inertness to basic compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Methanol : 10mM Phosphate buffer(pH6.8)
=30 : 70
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 254 nm
Inertsil ODS-EP
Peak identification
1. Uracil
2. Pyridine
3. Phenol0 2 4 6 8 10 12 14
Time (min)
1
2
3
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-EP
Comparison of inertness to pyridine between Inertsil ODS-EP and
commercial embedded columns
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8 10 12
Time (min)
0 2 4 6 8 10 12
Time (min)
Pyridine
Phenol
Pyridine Phenol
Pyridine
Phenol
Pyridine Phenol
Pyridine
Phenol
Pyridine
Phenol
0 2 4 6 8 10 12 14
Time (min)
(5) Acid test sample
~ for measurement of inertness to acidic compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
0.1% Phosphoric acid(pH2.2)
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 210 nm
Inertsil ODS-EP
Peak identification
1. Formic acid
2. Acetic acid1.0 2.0 3.0 4.0 5.0
Time (min)
1
2
1.0 2.0 3.0 4.0 5.0
Time (min)
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-EP
1.0 2.0 3.0 4.0 5.0
Time (min)
1.0 2.0 3.0 4.0 5.0
Time (min)
1.0 2.0 3.0 4.0 5.0
Time (min)
1.0 2.0 3.0 4.0 5.0
Time (min)
1.0 2.0 3.0 4.0 5.0
Time (min)
Formic acid
Acetic acid
Formic acid
Formic acid
Formic acid
Acetic acid
Acetic acid
Acetic acid
Formic acid
+Acetic acid
Acetic acid
Comparison of inertness to acids between Inertsil ODS-EP and
commercial embedded columns
(6) Oxine-copper sample
~ for measurement of inertness to chelating compounds ~
Conditions
Column dimensions:
4.6 mm I.D x 150 mm length
Mobile phase:
Acetonitrile : 0.1% Phosphoric acid(pH2.2)
=10 : 90
Flow rate: 1 ml / min
Column temperature: 40℃Detection: UV 254 nm
Inertsil ODS-EP
Peak identification
1. Oxine-copper1.0 2.0 3.0
Time (min)
1
Symmetry Shield RP18
Xterra RP18
Discovery RP Amide C16
Zorbax Bonus-RP
Hypersil HyPURITY Advance
Inertsil ODS-EP
Comparison of inertness to oxine-copper between Inertsil ODS-EP and
commercial embedded columns
1.0 2.0 3.0
Time (min)
1.0 2.0 3.0
Time (min)
1.0 2.0 3.0
Time (min)
1.0 2.0 3.0
Time (min)
1.0 2.0
Time (min)
1.0 2.0 3.0
Time (min)
0
20
40
60
80
100
0 50 100Flow time of 0.1% Phosphoric acid (pH2) ( hours )
Rel
ativ
e re
tent
ion
tim
e of
naph
thalen
e(%
)
Inertsil ODS-EP
ODS test column
Amide test column
Comparison of durability among Inertsil ODS-EP, ODS column
and Amide column
Test condition
Column: 4.6 mm I.D. x 150 mm
Flow rate: 1 ml / min
Column temperature: 30℃
Inertsil ODS-EP
● The selectivity is completely different from those of conventional
columns such as ODS column, but the retention is close to conventional
columns.
● High durability can be obtained even if strong acid was used as mobile
phase.
● Superior peak shapes of both acids and bases can be obtained because of
neutral bonded phase and low residual silanol groups.
● Silica gel of high-purity is used as the support material, which
minimizes effects of any remaining metal ions.
Inertsil ODS-EP is useful for the samples which are not separated by
conventional columns.
Inertsil ODS-EP is useful for the LC/MS analysis.