I N F O R M A T I O N FOR CONTRIBUTORS (1997)

A i m s and Scope

Genes & Development welcomes high-quality research papers of general interest and biological significance in molecular bi­ology, molecular genetics, and related areas. Publication t ime from acceptance of manuscript is between two and three months. For papers accepted subject to revision, only one re­vised version will be considered; it must be submitted within 2 months of the provisional acceptance.

Submiss ion of Papers The journal accepts papers which present original research that has not previously been published. Submission to the Journal implies that a paper is not currently being considered for an­other journal or book. Closely related papers that are in press elsewhere or that have been or will be submitted elsewhere should be included with the submitted manuscript. It is also understood that researchers who submit papers to this journal are prepared to make available to qualified academic researchers materials needed to duplicate their research results (DNA, cell lines, antibodies, microbial strains, and the like). Authors should submit nucleic acid and protein sequences to the appro­priate data bank.

Contributors from North and South America, Asia, and Aus­tralia should submit their papers to the Cold Spring Harbor Laboratory office. Contributors from Europe and Africa should submit their papers to the Edinburgh office. Questions regarding papers should be directed to Judy Cuddihy, Managing Editor, at Cold Spring Harbor Laboratory (516-367-8492).

Manuscript Preparation General. Papers should be concise and conform to the follow­

ing length requirements. Accepted papers will occupy 5 up to 12 journal pages, and have a manuscript length of not more than 63,000 characters (including spaces). Authors are encouraged to submit short (5-8 pages) papers. The entire paper (including tables, figure legends, references, footnotes) should be typed double-spaced, be of letter quality, and should use a typeface of at least 11 point size. Label each page with the first author's name and page number. Submit five copies of the manuscript and at least four copies of original art. The cover letter should include: (a) the name, address, telephone number, and FAX number of corresponding author; (b) a statement that the manu­script has been seen and approved by all listed authors; (c) any specific requirements for art reproduction; and (d) the status of any permissions required. In addition to the hardcopies, ac­cepted papers should be supplied on SVi- or 5y4-inch floppy disks in IBM, Macintosh, or compatible formats as a " text" or ASCII file. Indicate on the disc: computer brand name, the soft­ware program the document is in, and the disc format.

Form. The following order should be followed: Title page, Abstract, Introduction, Results, Discussion, Methods, Ac­knowledgments, References, Tables, Figure legends. The Title page should include: (a) title; (b) all authors' full names; (c) all affiliations clearly indicated; (d) a shortened version of the title for use as a running head (maximum 45 characters); and (e) key

words (up to 6) for use in indexing. The Abstract should be about 200 words long and should summarize the aim of the report, the methodological approach, and the significance of the results. Methods will appear at the end of the paper and should be detailed enough to allow any qualified researcher to duplicate the results.

Figures and Legends. Five sets of figures should be sup­plied as high-quality glossy prints. Half-tones should be high-contrast, particularly in the case of gels, for the best reproduc­tion. Line drawings, graphs, charts, and chemical formulae should be professionally prepared and labeled. Indicate mag­nification with a bar scale. Multiple-part figures should be submitted as mounted, camera-ready composites. Authors submitting color figures as essential data for review with manuscripts undertake to pay the publication costs of four-color artwork. Price estimates are supplied upon acceptance of the paper.

The back of each figure should be labeled with the first author's name, figure number, and an indication of " top." The figures should be numbered consecutively in the order to which they are referred in the text. The size of the figures will be adjusted to fit the Joumal format, therefore please try to keep labels, symbols, and other call-out devices in proportion to the figure size and detail.

Figure legends should be brief and should not contain methods. Symbols indicated in the figure should be identified in the legend text. If figures are reprinted from another source, permission to reprint is required.

Tables. Tabular data should be presented concisely and logi­cally. Tables should be numbered consecutively according to the order cited in the text and each should have a title. Use only horizontal rules and make sure column headings are un­ambiguous in indicating columns to which they refer. Table legends and footnotes should be included where needed. If tables are reprinted from another source or if data included are from another source, permission to reprint is required.

References. References are name/date citations in text; please do not cite by number. Only those articles that are published or in press will be included in the references. Un­published data, submitted manuscripts, or personal commu­nications should appear in the text and should include first initials and last names of authors. Please observe the follow­ing reference style:

Young, P., A.M. Richman, A.S. Ketchum, and D.P. Kiehart. 1993. Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function. Genes 8k Dev. 7: 29-41.

Robinow, C.F. and J.S. Hyams. 1989. General cytology of fis­sion yeasts. In The molecular biology of the fission yeast (ed. A. Nasim, P.G. Young, and B.F. Johnson), pp. 273-331. Academic Press, New York, NY.

Proofs. Proofs are considered the final form of the paper and correction can be made only in the case of factual errors. If additional information must be added at this stage, it should be in the form of "Note added in proof," subject to the ap­proval of the editors.

Reprints. A reprint order form will be included with the proofs.

To help defray the cost of publication, a charge of $30 per page will be made for publication in Genes &. Development. Authors unable to meet these charges should include a letter of explanation upon acceptance for publication; inability to meet these charges will have no effect on acceptance and publication of submitted papers.

BRAIN MAPPING July 8 -14 (Lecture Course) John Mazziotta, UCLA School of Medicine Arthur Toga, UCLA School of Medicine

The aim of this lecture course is to describe the rapidly evolving developments in brain mapping that have been applied to the problem of mapping the structure and function of the brain, both to understand its normal function and to evaluate neurological, neurosurgical and psychiatric disease states. This course describes new methods as well as the application of traditional techniques to the study of brain structure and function. Methodologies that will be discussed include magnetic resonance imaging (including functional, spectroscopic and angiographic approaches), positron emission tomography, electrophysiological techniques, optical intrinsic signal imaging, digital approaches to conventional postmortem neuroanatomical investigations, data analysis, statistical analysis, statistical approaches, visualization and stereotaxy. The course is not designed to simply describe methods, but rather to discuss how brain mapping strategies can be employed in combination with biological models for understanding the structure and function of the brain. Findings relevant to the function of the visual, motor, language, memory and cognitive brain systems, as well as diseases that adversely affect them, will be discussed. Specific hypotheses and experimental designs will be developed by the students for mock execution, or as an actual experiment if a field trip to an imaging laboratory can be arranged. Invited speakers will include world leaders in each of the respective brain mapping subspecialties.

SUMMER - THIRD SESSION COURSES

EUKARYOTIC GENE EXPRESSION July 23 - August 12

Michael Carey, UCLA School of Medicine Grace Gill, Harvard Medical School David Gilmour, Pennsylvania State University Stephen Smale, UCLA School of Medicine

The rapid cloning of eukaryotic promoters and regulatory factors has led to a dramatic increase in studies of gene regulation. The Eukaryotic Gene Expression course was designed for students, postdocs, and professors who have recently ventured into this dynamic area of contemporary biology. The course will focus on state-of-the-art strategies and techniques that are employed to study gene expression, with a special emphasis on transcriptional regulation. Regulation of transcription by nuclear proteins will be examined by in vitro transcription in cell-free extracts and by transfection of mammalian tissue culture cells. Analytical techniques for measuring gene expression will include primer extension and nuclease protection measurements of mRNAs, and enzymatic analyses of reporter proteins.

To accomodate the needs of many researchers, a strong emphasis will be placed on biochemical studies of protein-DNA interactions. Transcription factors will be expressed in a heterologous system such as E. coli or baculovirus and purified by affinity chromatography. A detailed characterization of the DNA-binding properties of the recombinant factors will be carried out using mobility shift, DNase I footprinting, and methylation interference assays. Over the past few years, the gene regulation field has begun to emphasize the importance of in vivo approaches to studying protein-DNA interactions. Students will therefore be instructed in the most advanced in vivo footprinting and UV crosslinking methodologies. Students will also learn strategies and techniques for executing a structure-function study of a regulatory factor, including an introduction to site-directed mutagenesis, basic computer analyses, and the two-hybrid approach in S. cerevisiae for studying protein-protein interactions. Experience with basic recombinant DNA techniques is a prerequisite for admission to this course. Lectures by the instructors will cover the current status of the gene expression field, theoretical aspects of the methodology, and broader issues regarding strategies for investigating the regulation of gene expression in eukaryotes.

Guest lecturers will discuss contemporary problems in eukaryotic molecular biology and technical approaches to their solution. Last year's speakers included S. Bell, S. Burley, J. Conaway, L.Preedman, S. Ghosh, B. Graves, M. Green, C. Peterson, R. Sen, B. Stillman, K. Struhl, N. Tanese, and K. Yamamoto.

IMAGING STRUCTURE & FUNCTION IN THE NERVOUS SYSTEM July 23 - August 12 Arthur Konnerth, University of Saarland, Germany Fred Lanni, Carnegie-Mellon University Raphael Yuste, Columbia University

Advances in light microscopy, digital image processing, and the development of a variety of powerful fluorescent probes present expanding opportunities for visualizing and measuring the structure and function of neurons, synapses, and networks in the brain. This intensive laboratory and lecture course will provide participants with the theoretical and practical tools to utilize these emerging technologies. The primary emphasis of the course will be on light microscopy, including fluorescence, differential interference and phase contrast, confocal scanning, and 2-photon scanning, as well as the use of different types of electronic cameras and the application of digital processing to enhance and analyze microscope images. Students will learn the principles of light microscopy, the use of calcium-sensitive probes (e.g. Fura-2), voltage-sensitive dyes, photo-activated ("caged") compounds, exocytosis tracers, whole-cell patch-clamp methods, single-cell microinjection of fluorescent indicators, and other methods to explore neuronal function. We will use a variety of neural systems, including living animals, brain slices (e.g. hippocampus, cerebellum, and neocortex), and cultured cells. Applicants should have a strong background in the neurosciences or in cell biology.

Lecturers in the 1996 course included: G. Augustine, W. Betz, T. Bonhoeffer, M. Chalfie, H. Cline, W. Denk, P. Forscher , S. Eraser, A. Grinvald, T. Hazelrigg, T. Inoue, L. Katz, J. Lichtman, R. Malinow, E. Neher, D. O'Malley, W. Regehr, R. Tsien, W. Webb and R. Wong.

YEAST GENETICS July 23 - August 12 Alison Adams, University of Arizona, Tucson Dan Gottschling, Fred Hutchinson Cancer Research Center Tim Stearns, Stanford University

The major laboratory techniques used in the genetic analysis of yeast will be studied, including the isolation and characterization of mutants, tetrad analysis, complementation, and initotic recombination. Micromanipulation used in tetrad analysis will be carried out by all students. Molecular genetic techniques, including yeast transformation, gene replacement, analysis of gene fusions, and generation of mutations in cloned genes, will be studied. Indirect immunofluorescence experiments will be done to identify the nucleus, microtubules, and other cellular components. Lectures on fundamental aspects of yeast genetics will be presented along with seminars given by outside speakers on topics of current interest.

THE BIOLOGY OF MEMORY: FROM MOLECULES TO BEHAVIOR July 18 - 31 (Lecture Course) Jack Byrne, University of Texas Medical School, Houston Howard Eichenbaum, Boston University Keir Pearson, University of Alberta, Canada Larry Squire, University of California, San Diego

This lecture course provides an introduction to cell, molecular and systems approaches to learning and memory. It is suited for graduate students in molecular biology, neurobiology, and psychology as well as research workers who are interested in an introduction to this field. The course will cover topics ranging from behavioral considerations of learning and memory to gene regulation in the nervous system. The lectures provide an intensive coverage of six selected areas: 1) an introduction to modern behavioral studies of learning and memory; 2) an overview of the cell biology of neuronal plasticity and second messenger systems; 3) the regulation of gene expression; 4) cellular and molecular mechanisms of simple forms of learning and memory in invertebrates and vertebrates; 5) cellular and molecular mechanisms of long-term potentiation and depression in various regions of the mammalian brain; and 6) systems approaches to learning in vertebrates and humans.

Past lecturers have included: B. Ball, M. Bear, A. Doupe, H. Eichenbaum, C. Gilbert, W. Greenough, P. Holland, E. Kandel, M. Mauk, P Pfaffinger, M. Raichle, C. Rankin, H. Schulman, J. Steinmetz, and T. TuUy.

NEUROBIOLOGY OF HUMAN NEUROLOGICAL DISEASE: Mechanisms of Neurodegeneration August 4 - 1 0 (Lecture Course)

Sam Gandy, Cornell University Medical College Sangram Sisodia, John Hopkins Univ. School of Medicine

How and why do neurons die in specific acute or chronic human neurological disorders? What are the molecular and biochemical manifestations of specific genetic lesions in specific neurodegenerative disorders? Do different pathological deaths share common mechanisms? What practical treatments can be contemplated? This lecture course will explore possible answers to these important questions. Recent advances in neurogenetics and in molecular and cell biology have begun to shed light on the mechanisms that underly nervous system injury in disease states such as Alzheimer's disease, amyotrophic lateral sclerosis, prion diseases, Huntington's disease, epilepsy and stroke. Taking advantage of small class size and extensive discussion, invited faculty lecturers will examine critical issues in their areas of expertise. Overview will be provided and course participants need not have familiarity with neurological diseases. The course will focus principally on the specific hypotheses and approaches driving current research. Emphasis will be placed on the highly dynamic interface between basic and clinical investigation, including the interdependence of clinical research and disease model development, and the value of disease research in understanding the function of the normal nervous system. Last year's faculty were D. Bredesen, D. Choi, V. Dawson, R. Edwards, S. Gandy, N. Heintz, J. McNamara, W. Mobley, D. Price, S. Prusiner, C. Ross, S. Sisodia, D. Selkoe and R. Tanzi.

FALL COURSES ADVANCED IN SITU HYBRIDIZATION & IMMUNOCYTOCHEMISTRY October 14 - 27 John Murray, Robert Ochs, Thomas Ried, David Spector MACROMOLECULAR CRYSTALLOGRAPHY October 14 - 27 William Furey, Gary Gilliland, Alexander McPherson, James Pflugrath

POSITIONAL CLONING: CONTIG TO CANDIDATE GENE October 14 - 27 Roger Reeves, Gary Silverman MOLECULAR AND CELL BIOLOGY OF S. POMBE & OTHER YEASTS November 4 - 17 Tom Chappell, Paul Young MOUSE BEHAVIORAL ANALYSIS November 4-17 Instructors to be announced

PHAGE DISPLAY OF COMBINATORIAL ANTIBODY LIBRARIES November 4-17 Carlos Barbas, Dennis Burton, Gregg Silverman

COMPUTATIONAL GENOMICS November 6-11 William Pearson, Randall Smith

Suiiiiner C'lntrse Appliciiihiti D-^'adtim March 15th, 1997

tall i'oiirse Applicatkni Dt'tiilliiw: July 15th, 1997

Visit our web site far details on how to apply, prices and possible scholarship support:

Meetings & Courses Office Cold Spring Harbor Laboratory

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fax 516 367 8845 * meetings@cshl.org http://wwwxshl.org/meetings/

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