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Supplementary Data
British Journal of Cancer
Inhibition of neurotensin receptor 1 induces intrinsic apoptosis
through let-7a-3p/Bcl-w axis in glioblastoma
Zhen Dong, Qian Lei, Rui Yang, Shunqin Zhu, Xiao-Xue Ke, Liqun Yang, Hongjuan
Cui, and Liang Yi
Supplemental Figures
Supplementary Figure S1 NTSR1 inhibitor SR48692 inhibited cell viability in
glioblastoma cells. (A) Real-time RT–PCR analysis of ntsr1 expression in U87,
LN229, A172 and U251 cells, and a primary glioblastoma tissue 0807T as well as a
counterpart para-carcinoma tissue 0807P. (B) Cells were treated with 0.001, 0.01, 0.1,
1, 10, 100, 1000 μM SR48692 for 48 hours, and inhibition rates were analyzed by
MTT assay. IC50 was calculated by using a GraphPad Prism 6. (C) Image of JC-1
staining in GL261, A172 and U251 glioblastoma cells after treatment of 10μM
SR48692 or DMSO for 48 hours. CCCP treatment groups was used as a positive
control. Scale bars represent 100 μm. All data are shown as the mean ± SD. Student’s
t-test was carried out. ***p < 0.001.
Supplementary Figure S2 (A) Real-time RT–PCR analysis of ntsr1 expression in
A172 and U251 glioblastoma cells after NTSR1 knockdown. (B) Representative
western blot of NTSR1 expression in A172 and U251 glioblastoma cells after NTSR1
knockdown. GAPDH expression levels served as a loading control. (C) Cell viability
assay in A172 and U251 glioblastoma cells after NTSR1 knockdown. All data are
shown as the mean ± SD. Student’s t-test was carried out. **p < 0.01, ***p < 0.001.
Supplementary Figure S3 (A) The effect of Annexin V-FITC/PI dual staining
apoptosis assays analyzed by flow cytometry in A172 and U251 cells after scramble
or NTSR1 knockdown in the presence or absence of 0.5 μM doxorubicin in cultural
medium for 48 hours. (B) The effect of relative caspase 3 activities assays in A172
and U251 cells after scramble or NTSR1 knockdown in the presence or absence of 0.5
μM doxorubicin in cultural medium for 48 hours. 20 μM Ac-DEVD-CHO, a caspase3
inhibitor, was used to perform a negative control. All data are shown as the mean ±
SD. Student’s t-test was carried out. *p < 0.05, **p < 0.01, ***p < 0.001, n.s., no
sense.
Supplementary Figure S4 (A) The effect of Annexin V-FITC/PI dual staining
apoptosis assays analyzed by flow cytometry in 10 μM SR48692 or DMSO-treated
U251 cells after transient transfected with Bcl-w for 48 hours. (B) The effect of
Annexin V-FITC/PI dual staining apoptosis assays analyzed by flow cytometry in
scramble or NTSR1-silenced U251 cells after transient transfected with Bcl-w for 48
hours. All data are shown as the mean ± SD. Student’s t-test was carried out. ***p <
0.001.
Supplementary Figure S5 Heatmap of microRNA array in U251 cells after NTSR1
knockdown in the absence or presence of actinomycin D in cultural medium for 24
hours.
Supplementary Figure S6 (A) Relative expression of let-7a-3p in A172 and U251
cells after NTSR1 knockdown. (B) Relative expression of let-7a-3p in A172 and
U251 cells after 10 μM SR48692 treatment. (C) Relative expression of let-7a-5p in
A172 and U251 cells after NTSR1 knockdown. (D) Relative expression of let-7a-5p
in A172 and U251 cells after10 μM SR48692 treatment. (E) Relative expression of
c-Myc and LIN28 in A172 and U251 cells after10 μM SR48692 treatment. All data
are shown as the mean ± SD. Student’s t-test was carried out. *p < 0.05, **p < 0.01,
***p < 0.001, n.s., no sense.
Supplementary Figure S7 (A) A172 cells were injected into the flank of BALA/c
nude mice and treated with DMSO or 10mg/kg SR48692 by subcutaneous injection
when tumors were palpable. Mice weight was measured. (B) scramble or
NTSR1-silenced A172 cells were injected into the flank of BALA/c nude mice and
treated with 5μg/kg actinomycin D by subcutaneous injection when tumors were
palpable. Mice weight was measured. All data are shown as the mean ± SD. Student’s
t-test was carried out. *p < 0.05, **p < 0.01, ***p < 0.001.
Supplementary Tables
Supplementary Table S1. RT-PCR primers
Primer
set
Forward Reverse
ntsr1 CGTGGAGCTGTACAACTTCA CAGCCAGCAGACCACAAAGG
c-myc GGGTTCACATGCCCTACACT GGAATCTGGCACTTTTGGCA
lin28 TGATGCAGAAGATCACTCCGTT GGGCTTCCCTCTCGGTTTAT
β-actin CTCTGCTCCTCCTGTTCGAC GCGCCCAATACGACCAAATC
Supplementary Table S2. RNAi target sequences
Target Sequence
shNTSR1#1 AAGAAGTTCATCAGCGCCATC
shNTSR1#2 AACACCTTCATGTCCTTCATA
scramble ATCCGTCCGAACGTAAGTCAA
Supplementary Table S3. PCR primers
Primer set Forward Reverse
Bcl-w CCGGAATTCATGGCGA
CCCCAGCCTCG
CGCGCGGCCGCTCACTTGCTAGCAA
AAAAGGCCC
Supplementary Table S4. Bcl-w 3’UTR sequences
Name Sequence
Bcl-w 3’UTR-wild GGGGGTCTCTGACTTGCTCAGGACAAACTAGGC
CAGTGGTTTTCAAACTGCTTGGCAGAGCCCTGA
AGTTTCCTAGGGGTTGCCTCAGGAGTCCTTGGG
GAGATGAAGGGGGTGGGGAGCTGAGCAGGCTG
GGCAATTTGCCCTCAAACAGAACAGCTCCCCTT
GTAGCGTGATTACATATTGGGGTTCAGGGTAAG
ATTTTATTTGCATTAAGGGGTTTGCTGCTGAAAA
AAAGTTGGAAAACCACTGACTAGACCATCGGCT
CCAAATTGG
Bcl-w 3’UTR-mutant GGGGGTCTCTGACTTGCTCAGGACAAACTAGGC
CAGTGGTTTTCAAACTGCTTGGCAGAGCCCTGA
AGTTTCCTAGGGGTTGCCTCAGGAGTCCTTGGG
GAGATGAAGGGGGTGGGGAGCTGAGCAGGCTG
GGCAATTTGCCCTCAAACAGAACAGCTCCCCTT
GTAGCTGTCTTACATATTGGGGTTCAGGGTAAG
ATTTTATTTGCATTAAGGGGTTTGCTGCTGAAAA
AAAGTTGGAAAACCACTGACTAGACCATCGGCT
CCAAATTGG
Supplementary Table S5. Signal values of RayBio® Human Apoptosis Antibody
Array applied in U251 cells after scramble or NTSR1 knockdown in the presence
or absence of actinomycin D.
name scramble shNTSR1#1 scramble+actD shNTSR1#1+actD p value* p value**
BID 12 11 12 12 11.5 10 12 10 10 11 12 9 0.5072 > 0.9999
BIM 1225 1239 1231.5 1274 1248 1329 1177 1208 1140 1134 1123 1140 0.0990 0.1032
CD40 250 221 239 365.5 375.5 374.5 95 86 91.5 135 159.5 158.5 0.0001 0.0020
CD40L 798 724 776 798 828 788.5 633 632 592 780 734 712 0.1947 0.0070
Caspase3 53 58 55.5 91.5 74.5 88.5 538.5 542 457 1545.5 1524 1452 0.0057 <0.0001
DR6 1236 1247 1232 956 1127 1018 910 882 912.5 719 892 877 0.0151 0.2684
Fas 3541 3591 3552 4190 3769.5 3976 3741.5 3681 3761.5 3190 3412.5 3271.5 0.0270 0.0033
FasL 259 307 294.5 179.5 205 189 122 132.5 111.5 98.5 112 108.5 0.0041 0.0980
HSP27 734.5 821 793 659 677 679 743 688 664 542.5 413 472 0.0133 0.0073
HSP60 8532 8589.5 8567.5 17120 13892 14195 14289 13298 14708 16128 13482 15779 0.0032 0.3293
HSP70 2123 2131 2150.5 467 456 435 2328 2390.5 2401.5 3599 3682 3698.5 <0.0001 <0.0001
HTRA 8902.5 8814 8863.5 7125.5 7893 7790 7823 8220 8340.5 8340.5 8599 8498.5 0.0065 0.1127
IGF-1sR 3123 3434 3252 3654 3289 3580.5 1518 1712 1666.5 1780 1830 1907 0.1721 0.0405
IGF-I 121 201 153 120 135.5 123.5 84 88.5 83.5 88 79.5 89 0.2486 0.9633
IGF-II 588.5 634 613 890 885 840 888 812 837 745 702 703.5 0.0002 0.0082
IGFBP-1 645 534 573 633 682.5 642.5 644.5 623 651.5 480 477 476 0.1281 <0.0001
IGFBP-2 1010 835.5 944 2106 2016 2118 439 428.5 418 582.5 599 588 <0.0001 <0.0001
IGFBP-3 267.5 243.5 229.5 189.5 203 179.5 89 72 61.5 112 114 108.5 0.0125 0.0103
IGFBP-4 189 225 250 143 110.5 120.5 123 138 120.5 98.5 130 100.5 0.0087 0.2045
IGFBP-5 435.5 423 424.5 231 236 246 193.5 134 180 219.5 256 223.5 <0.0001 0.0406
IGFBP-6 578 562.5 564.5 489 558.5 504 488 472.5 465.5 433.5 491 457 0.0776 0.4554
SMAC 9345.5 9878.5 9972 15283.5 13489 14438 12378.5 13489 13709.5 10342 13458.5 10257.5 0.0011 0.1791
Survivin 26341 24319 25016 21897.5 24719 23610 2239.5 2139.3 2254.5 3200 3498 3198 0.1473 0.0005
TNF-alpha 513 548 551.5 306 284 296.5 388 372.5 399.5 430 459 441 <0.0001 0.0079
TNF-beta 881.5 905.5 903.5 633 645.5 651.5 823.5 845 824.5 634.5 598 614 <0.0001 <0.0001
TRAILR-1 569.5 496.5 475.5 533.5 502 487 329 324 347 312 379 354.5 0.8510 0.5059
TRAILR-2 134 189.5 156 124 109 105.5 9910 1020.5 9950.5 3456.5 3490 3129.5 0.0515 0.2923
TRAILR-3 10.5 11 9.5 11 8 10 31 33 32 50 52 53.5 0.5360 <0.0001
TRAILR-4 486.5 563.5 505 332 378 351 352 345.5 359.5 320.5 335.5 314 0.0035 0.0184
XIAP 19847.5 18934.5 20196.5 30123 31245.5 30920 1422 1388.5 1411.5 3289 3120 3103 <0.0001 <0.0001
Bad 32.5 31 29 11 11 10 9 8 10 7.5 10 9 <0.0001 0.8662
Bax 452.5 498 463.5 472 428 477 356 377 369 356 342.5 340.5 0.5841 0.0549
bcl-2 210.5 235 212 152 144 140 167.5 143 160.5 78 93 89.5 0.0010 0.0012
bcl-w 162.5 178 169.5 78 82 81 137 123 126 80 98 82 < 0.0001 0.0041
cIAP-2 226 238.5 217 122 136 112.5 120.5 134.5 136.5 123 129 137 0.0004 0.9036
caspase8 10.5 8 12 14 11 8 12 12 10 10 11 9 0.7102 0.2051
cytoC 145 132 138 218 228 217 66 65.5 61 74.5 89 85.5 < 0.0001 0.0155
Livin 163.5 159.5 163.5 49 53.5 50 15 13 14 10 8 9 < 0.0001 0.0036
p21 3765.5 3782 3743.5 8124 8208 8137.5 3879 3589.5 3934.5 14590 14328.5 15172 < 0.0001 < 0.0001
p27 297.5 365 330 276 300 279 92.5 87.5 95 228.5 249 250 0.0934 < 0.0001
p53 30128 29987 30074.5 24178 22454 23274 5353 5120 5313 7324.5 7274 7271.5 0.0002 < 0.0001
sTNF-R1 658.5 688 634.5 796 778 796 532 504 502 419.5 423 427 0.0014 0.0008
sTNF-R2 419 459.5 429.5 138 188.5 156 288 196 211 332 267 222.5 0.0001 0.3793
* scramble vs. shNTSR1#1
** scramble+actD vs. shNTSR1#1+actD
Supplementary Table S6. Relative signal values of human microRNA array
applied in U251 cells after scramble or NTSR1 knockdown in the presence or
absence of actinomycin D.
name scramble shNTSR1#1 scramble+actD shNTSR1#1+actD p
value*
p
value**
miR-298 0.892 0.993 1.115 0.321 0.223 0.264 4.982 5.637 5.287 0.452 0.433 0.464 0.0005 < 0.0001
miR-453 0.882 1.103 1.015 0.752 0.783 0.772 0.783 0.803 0.781 0.984 0.932 0.891 0.0236 0.0062
miR-663 0.892 1.104 1.004 0.198 0.245 0.215 7.203 7.123 6.910 0.613 0.635 0.600 0.0002 < 0.0001
miR-675-5p 1.023 0.953 1.024 1.084 0.993 1.043 5.503 5.430 5.618 0.884 0.892 0.844 0.3202 < 0.0001
miR-718 0.934 0.893 1.173 0.221 0.239 0.283 2.359 2.438 2.461 0.263 0.264 0.245 0.0011 < 0.0001
miR-762 1.078 0.945 0.977 1.309 1.189 1.290 1.104 1.128 1.186 0.683 0.694 0.673 0.0086 < 0.0001
let-7a-5p 0.894 0.955 1.151 0.789 0.845 0.820 0.443 0.508 0.456 0.791 0.789 0.746 0.0831 0.0002
let-7a-3p 0.998 0.958 1.044 14.673 18.823 16.156 2.994 3.123 3.083 9.416 10.195 8.444 0.0002 0.0002
let-7b 1.046 1.032 0.922 0.320 0.389 0.370 0.556 0.549 0.549 0.340 0.359 0.307 0.0001 0.0001
let-7c 0.94 0.994 1.066 0.508 0.455 0.447 0.837 0.884 0.868 0.683 0.664 0.681 0.0002 0.0002
let-7d 0.795 1.127 1.078 1.453 1.478 1.410 1.556 1.478 1.579 1.601 1.583 1.603 0.0132 0.1367
let-7e 0.884 0.994 1.122 0.603 0.552 0.583 0.883 0.863 0.861 1.173 1.183 1.178 0.0039 < 0.0001
let-7f 1.092 1.034 0.874 0.903 0.826 0.835 0.893 0.793 0.870 1.144 1.237 1.143 0.1049 0.0018
let-7g-5p 0.886 0.992 1.122 0.443 0.394 0.401 1.339 1.324 1.323 1.045 1.022 1.060 0.0011 < 0.0001
let-7g-3p 0.984 1.109 0.907 0.612 0.689 0.666 0.538 0.525 0.538 0.573 0.723 0.564 0.0055 0.1706
let-7i-5p 0.956 0.985 1.059 0.384 0.438 0.400 0.992 0.973 0.957 0.887 0.894 0.866 < 0.0001 0.0022
let-7i-3p 0.924 0.992 1.084 0.705 0.793 0.773 0.835 0.823 0.822 0.989 1.045 0.911 0.0105 0.0166
miR-100 1.071 0.975 0.954 0.583 0.672 0.603 2.132 2.098 2.111 0.772 0.890 0.722 0.0011 < 0.0001
miR-103 1.082 1.023 0.895 1.289 1.324 1.316 2.189 2.342 2.585 2.335 2.458 2.343 0.0053 0.9590
miR-106a-5p 1.011 1.048 0.941 1.777 1.554 1.681 2.327 2.184 2.191 2.334 2.198 2.233 0.0007 0.7514
miR-106a-3p 1.019 1.048 0.933 1.932 1.610 1.792 2.219 1.093 2.198 1.375 1.428 1.441 0.0014 0.3205
miR-106b-5p 1.061 0.932 1.007 1.427 1.623 1.576 3.004 3.126 2.924 2.384 2.295 2.249 0.0015 0.0006
miR-106b-3p 0.083 0.994 1.923 0.589 0.683 0.665 0.378 0.421 0.391 1.302 1.149 1.291 0.5418 < 0.0001
miR-107 0.998 0.98 1.022 0.465 0.423 0.439 0.618 0.643 0.631 0.458 0.448 0.442 < 0.0001 < 0.0001
miR-10a 1.034 0.979 0.987 0.772 0.734 0.742 1.004 1.008 0.933 1.097 1.038 1.086 0.0003 0.0388
miR-10b 1.041 0.993 0.966 0.774 0.765 0.775 2.192 1.428 1.751 0.694 0.683 0.688 0.0005 0.0076
miR-1197 0.984 0.893 1.123 0.284 0.342 0.303 2.231 2.321 2.179 0.629 0.518 0.514 0.0006 < 0.0001
miR-122-5p 0.972 0.895 1.133 0.507 0.449 0.474 5.221 4.668 5.219 2.893 2.945 2.944 0.0019 0.0003
miR-122-3p 0.918 1.045 1.037 0.603 0.504 0.559 1.386 1.438 1.415 0.891 0.930 0.823 0.0009 0.0001
miR-124-3p 0.91 1.082 1.008 0.012 0.008 0.010 0.443 0.349 0.424 0.081 0.043 0.014 < 0.0001 0.0005
miR-125a-3p 0.951 1.074 0.975 1.302 1.232 1.224 1.332 1.034 1.154 0.983 1.129 1.101 0.0050 0.3529
miR-125a-5p 0.955 1.098 0.947 1.205 1.258 1.232 0.775 0.746 0.782 0.836 0.983 0.865 0.0108 0.0517
miR-125b-3p 0.942 0.993 1.065 1.004 0.835 0.973 1.023 1.102 1.165 0.738 0.718 0.769 0.3763 0.0012
miR-125b-5p 0.955 0.93 1.115 0.865 0.821 0.841 1.304 1.203 1.181 1.132 1.093 1.131 0.0565 0.0505
miR-126-3p 0.957 0.945 1.098 2.109 2.543 2.359 3.320 3.043 3.029 2.549 2.451 2.658 0.0006 0.0067
miR-126-5p 1.023 0.942 1.035 0.225 0.240 0.228 1.773 1.568 1.740 0.597 0.583 0.589 < 0.0001 < 0.0001
miR-1281 1.045 0.974 0.981 0.583 0.515 0.521 11.320 10.932 11.955 2.331 2.355 2.435 0.0001 < 0.0001
miR-129-3p 1.052 0.892 1.056 0.745 0.802 0.784 0.128 0.203 0.167 0.329 0.209 0.210 0.0169 0.1399
miR-129-5p 0.993 1.034 0.973 0.887 0.914 0.904 3.134 2.894 3.030 1.218 1.097 1.101 0.0074 < 0.0001
miR-130a 1.034 0.983 0.983 0.254 0.202 0.232 0.781 0.614 0.606 0.184 1.203 0.199 < 0.0001 0.7065
miR-130b 1.041 0.979 0.98 0.134 0.112 0.129 1.818 1.893 1.863 0.242 0.136 0.142 < 0.0001 < 0.0001
miR-132 1.011 0.894 1.095 3.023 3.419 3.279 6.302 6.230 5.986 5.672 5.416 5.627 < 0.0001 0.0084
miR-135b-5p 1.047 0.887 1.066 1.225 1.378 1.338 0.892 0.833 0.822 0.719 0.732 0.726 0.0126 0.0050
miR-135b-3p 1.083 0.918 0.999 1.223 1.089 1.111 1.134 1.523 1.627 1.384 1.459 1.466 0.0894 0.9590
miR-138 1.032 0.925 1.043 1.235 1.119 1.222 1.004 0.893 1.030 0.884 0.938 0.913 0.0217 0.2264
miR-139-3p 0.991 1.038 0.971 0.342 0.315 0.322 1.034 1.009 1.179 0.418 0.443 0.439 < 0.0001 0.0003
miR-139-5p 0.999 1.092 0.909 1.359 1.132 1.290 1.112 1.231 1.280 0.908 0.892 0.909 0.0382 0.0037
miR-140 1 0.993 1.007 0.780 0.834 0.805 1.123 1.099 1.098 0.746 0.774 0.745 0.0003 < 0.0001
miR-141-3p 0.989 1.023 0.988 0.815 0.845 0.816 1.892 1.752 1.992 3.987 3.617 4.038 0.0003 0.0002
miR-141-5p 0.928 1.042 1.03 0.212 0.235 0.222 1.345 1.945 1.972 0.917 0.993 0.892 < 0.0001 0.0166
miR-142-3p 1.096 0.78 1.124 0.756 0.783 0.772 1.233 2.435 2.525 2.098 1.976 2.086 0.1064 0.9803
miR-142-5p 1.023 0.994 0.983 1.052 0.899 1.056 0.456 0.573 0.552 0.321 0.332 0.307 0.9670 0.0049
miR-143 1.103 0.982 0.915 0.583 0.702 0.689 0.432 0.342 0.321 0.295 0.328 0.294 0.0068 0.1731
miR-144 1.037 0.943 1.02 2.003 1.943 1.937 1.221 1.219 1.379 4.737 4.253 4.742 < 0.0001 < 0.0001
miR-145-5p 0.992 0.993 1.015 0.205 0.296 0.287 1.453 1.421 1.566 0.817 0.823 0.808 < 0.0001 0.0001
miR-1469 0.883 1.025 1.092 0.994 1.013 1.057 5.462 5.385 5.618 1.217 1.283 1.120 0.7570 < 0.0001
miR-146a 1.032 1.01 0.958 1.452 1.221 1.367 0.723 0.827 0.741 0.386 0.746 0.758 0.0081 0.3492
miR-146b-5p 1.045 1.024 0.931 0.203 0.225 0.195 1.002 1.045 1.067 0.185 0.203 0.183 < 0.0001 < 0.0001
miR-146b-3p 1.081 0.994 0.925 2.044 2.055 2.038 1.213 1.093 1.174 2.974 3.041 2.749 < 0.0001 < 0.0001
miR-147 0.892 1.061 1.047 2.504 2.336 2.468 2.003 2.132 2.140 2.001 1.930 2.077 < 0.0001 0.2209
miR-148a-3p 1.045 0.98 0.975 0.678 0.784 0.768 2.138 1.985 2.034 0.993 0.732 0.908 0.0030 0.0002
miR-148a-5p 0.789 1.026 1.185 0.045 0.083 0.072 1.234 1.323 1.343 0.112 0.114 0.116 0.0013 < 0.0001
miR-148b-3p 0.873 1.092 1.035 0.488 0.535 0.513 1.004 0.934 0.907 0.538 0.554 0.524 0.0019 0.0002
miR-148b-5p 0.998 1.055 0.999 1.045 1.052 1.033 0.482 0.655 0.600 0.793 0.775 0.794 0.2561 0.0154
miR-149 0.884 1.032 1.084 0.146 0.138 0.136 1.558 1.443 1.654 0.402 0.332 0.408 0.0001 < 0.0001
miR-150-5p 0.845 1.065 1.09 0.572 0.508 0.569 2.132 1.221 2.192 1.208 1.184 1.201 0.005 0.1071
miR-150-3p 0.877 1.023 1.1 0.671 0.655 0.666 1.893 2.345 2.704 0.784 0.775 0.796 0.0069 0.0029
miR-151-3p 1.077 1.022 0.901 0.673 0.653 0.676 2.783 2.226 2.866 1.428 1.482 1.550 0.0032 0.0051
miR-151-5p 1.099 0.99 0.911 0.702 0.640 0.684 0.892 1.032 0.920 0.583 0.593 0.596 0.0049 0.0011
miR-152 0.993 0.775 1.232 0.344 0.321 0.311 0.885 0.721 0.553 0.172 0.184 0.174 0.0070 0.0048
miR-154 0.987 0.994 1.019 0.665 0.603 0.624 0.332 0.293 0.280 0.233 0.203 0.186 < 0.0001 0.0105
miR-155 0.912 1.12 0.968 1.883 1.822 1.843 1.982 1.452 1.795 5.128 5.002 6.205 0.0002 0.0008
miR-15a-5p 0.923 1.312 0.765 2.341 2.672 2.512 1.337 1.328 1.341 0.799 0.983 0.744 0.0013 0.0024
miR-15a-3p 1.09 0.993 0.917 2.044 3.199 2.056 1.321 1.274 1.217 1.429 1.448 1.482 0.0207 0.0057
miR-15b-5p 1.003 0.83 1.167 0.224 0.402 0.383 0.884 0.714 0.712 0.592 0.438 0.461 0.0041 0.0215
miR-15b-3p 1.035 1.003 0.962 0.683 0.402 0.673 1.002 1.442 1.079 0.403 0.441 0.408 0.0118 0.0051
miR-16-1 0.984 0.83 1.186 0.442 0.459 0.443 1.034 1.321 1.284 0.628 0.647 0.676 0.0059 0.0035
miR-16-1-3p 0.996 0.956 1.048 0.506 0.634 0.447 1.821 1.784 1.841 0.884 0.982 0.887 0.0015 < 0.0001
miR-16-2-3p 0.925 1.042 1.033 1.456 1.438 1.376 1.632 1.347 1.562 1.453 1.618 1.649 0.0007 0.6007
miR-17-5p 1.008 0.987 1.005 0.447 0.700 0.482 1.283 1.221 1.273 0.423 0.214 0.485 0.0045 0.0005
miR-17-3p 1.023 1.034 0.943 2.109 1.835 2.218 0.663 0.615 0.648 1.904 1.626 1.943 0.0009 0.0003
miR-181a 1.03 1.032 0.938 0.668 0.759 0.649 1.223 1.043 1.041 0.735 0.788 0.762 0.0026 0.0054
miR-181b 1.039 0.993 0.968 0.735 0.874 0.739 0.934 1.003 0.903 0.771 0.782 0.642 0.0123 0.0162
miR-181c 0.993 0.934 1.073 2.667 2.785 2.558 3.320 3.231 3.383 3.788 3.209 3.697 < 0.0001 0.2429
miR-181d 0.984 0.927 1.089 0.243 0.229 0.225 1.232 1.221 1.285 0.997 1.138 0.952 < 0.0001 0.0217
miR-182 0.967 1.094 0.939 1.457 1.592 1.521 0.883 0.874 0.857 1.102 1.307 1.109 0.0011 0.0112
miR-183-5p 0.923 1.12 0.957 1.032 1.039 0.949 0.739 0.732 0.703 0.348 0.495 0.413 0.9259 0.0022
miR-183-3p 0.994 1.223 0.783 2.443 2.884 2.604 4.856 4.382 4.750 0.692 1.346 0.880 0.0008 0.0001
miR-184 0.782 1.094 1.124 0.020 0.018 0.005 0.456 0.536 0.428 0.038 0.119 0.011 0.0008 0.0008
miR-185 0.795 1.123 1.082 0.834 0.902 0.848 0.993 0.932 0.936 0.893 1.004 0.981 0.2580 0.8919
miR-186-5p 1.096 0.967 0.937 0.805 0.723 0.746 1.253 1.372 1.376 0.756 0.734 0.747 0.0114 0.0001
miR-186-3p 1.009 0.992 0.999 0.658 0.795 0.694 0.335 0.372 0.352 0.329 0.432 0.338 0.0023 0.7198
miR-187 1.003 0.923 1.074 0.251 0.398 0.220 3.528 3.438 3.414 0.517 0.629 0.760 0.0005 < 0.0001
miR-18a-5p 1.045 0.924 1.031 1.345 1.332 1.359 1.672 1.783 1.880 1.293 1.213 1.212 0.0009 0.0012
miR-18a-3p 0.973 1.003 1.024 0.993 0.892 0.991 0.893 1.023 0.806 0.703 0.736 0.704 0.3205 0.0393
miR-18b 0.945 1.045 1.01 3.341 3.954 3.201 1.003 1.042 1.044 2.004 2.194 3.066 0.0004 0.0131
miR-191-5p 1.094 0.892 1.014 0.882 1.092 0.896 0.892 0.873 0.837 0.783 0.776 0.778 0.6542 0.0056
miR-191-3p 1.093 0.884 1.023 0.255 0.263 0.276 1.212 1.093 1.127 0.548 0.553 0.631 0.0003 0.0002
miR-193b 0.943 1.004 1.053 0.927 1.003 0.967 1.763 1.582 1.636 1.648 1.638 1.653 0.4246 0.8076
miR-194 0.928 1.093 0.979 1.793 1.893 1.869 3.329 3.098 3.293 4.008 3.896 4.034 0.0001 0.0009
miR-195-5p 0.942 1.034 1.024 1.845 1.782 2.100 6.823 7.890 7.440 8.784 8.229 8.631 0.0009 0.0294
miR-195-3p 1.094 1.003 0.903 0.189 0.234 0.147 1.023 0.992 0.957 0.324 0.402 0.313 0.0002 < 0.0001
miR-196a 1.094 0.934 0.972 0.190 0.193 0.179 0.672 0.583 0.606 0.349 0.421 0.340 < 0.0001 0.0025
miR-196b 1.096 0.975 0.929 0.882 0.725 0.776 1.201 1.205 1.232 0.774 0.784 0.672 0.0389 0.0002
miR-198 0.934 0.983 1.083 0.672 0.683 0.690 1.572 1.882 1.747 2.086 2.381 2.059 0.0020 0.0320
miR-199b-3p 1.032 0.993 0.975 5221 4.523 4.218 6.334 6.273 6.439 7.482 6.894 7.532 0.0003 0.0106
miR-199a-3p 1.095 1.034 0.871 0.532 0.553 0.525 1.123 1.112 1.170 0.304 0.657 0.620 0.0023 0.0058
miR-199a-5p 0.883 0.993 1.124 0.255 0.265 0.260 0.893 0.735 0.790 0.783 0.658 0.636 0.0004 0.1558
miR-199b-5p 0.892 0.943 1.165 0.294 0.308 0.289 0.482 0.338 0.372 0.782 0.927 0.741 0.0011 0.0042
miR-19a 0.984 1.045 0.971 1.783 1.632 1.684 1.598 1.814 1.847 1.329 1.238 1.371 0.0001 0.0073
miR-19b 0.994 1.033 0.973 0.883 0.793 0.825 0.409 0.384 0.410 0.514 0.505 0.514 0.0063 0.0003
miR-200a-3p 0.782 1.112 1.106 0.935 0.994 0.929 2.013 2.118 2.228 3.219 3.198 3.337 0.6917 0.0001
miR-200a-5p 0.773 1.098 1.129 0.520 0.538 0.504 0.552 0.504 0.502 0.443 0.451 0.436 0.0138 0.0109
miR-200b-3p 0.68 1.201 1.119 0.993 0.932 0.940 1.012 1.230 1.116 2.148 2.098 2.152 0.7960 0.0001
miR-200b-5p 0.932 0.993 1.075 1.102 1.099 1.069 1.932 1.893 2.217 1.034 1.003 1.041 0.1030 0.0007
miR-200c-3p 0.56 1.307 1.133 2.103 1.994 1.969 1.231 1.034 1.303 1.098 1.002 1.099 0.0112 0.2285
miR-200c-5p 0.893 1.093 1.014 0.578 0.583 0.569 1.182 1.093 1.191 0.938 0.945 0.953 0.0019 0.0027
miR-202-3p 0.789 1.023 1.188 0.021 0.013 0.001 0.384 0.321 0.344 0.043 0.015 0.034 0.0010 < 0.0001
miR-202-5p 1.093 1.003 0.904 1.215 1.334 1.204 1.345 1.582 1.468 0.783 0.773 0.791 0.0216 0.0006
miR-204 1.124 0.933 0.943 0.206 0.221 0.181 0.832 0.452 0.739 0.302 0.227 0.226 0.0002 0.0226
miR-205 1.332 0.783 0.885 0.163 0.158 0.151 0.032 0.230 0.170 0.054 0.038 0.048 0.0075 0.1732
miR-206 1.093 0.955 0.952 4.203 4.321 4.238 3.321 3.582 3.388 12.309 11.237 12.056 < 0.0001 < 0.0001
miR-208b 1.212 0.821 0.967 0.394 0.455 0.451 4.592 4.772 4.700 0.803 0.784 0.865 0.0081 < 0.0001
miR-20a-5p 0.987 1.034 0.979 0.663 0.672 0.694 1.132 1.324 1.341 0.989 0.938 0.920 < 0.0001 0.0107
miR-20a-3p 1.007 1.003 0.99 0.502 0.457 0.485 1.592 1.482 1.548 1.528 1.556 1.525 < 0.0001 0.9032
miR-20b-5p 1.034 0.996 0.97 0.835 0.856 0.848 1.672 1.667 1.638 1.250 1.237 1.236 0.0014 < 0.0001
miR-20b-3p 1.213 0.773 1.014 0.934 0.793 0.800 0.443 0.345 0.319 1.308 1.295 1.386 0.3084 < 0.0001
miR-21-5p 1.435 0.789 0.776 0.645 0.693 0.680 1.325 1.321 1.297 1.023 1.003 0.926 0.2076 0.0006
miR-21-3p 1.032 0.934 1.034 1.503 1.427 1.475 0.882 0.903 0.888 0.538 0.783 0.536 0.0003 0.0297
miR-210 1.127 1.003 0.87 0.245 0.266 0.280 0.382 0.345 0.373 0.303 0.285 0.297 0.0006 0.0044
miR-211 1.156 0.787 1.057 1.020 1.003 1.017 2.414 2.519 2.512 1.098 1.045 1.080 0.9097 < 0.0001
miR-212-3p 0.984 0.993 1.023 0.803 0.732 0.726 2.394 2.243 2.326 1.892 1.845 1.833 0.0008 0.0006
miR-214-5p 1.045 1.034 0.921 1.283 1.209 1.277 1.628 1.842 1.544 1.457 1.442 1.427 0.0052 0.0618
miR-214-5p 0.995 1.034 0.971 0.604 0.559 0.597 1.342 1.034 1.135 0.889 0.783 0.889 < 0.0001 0.0312
miR-218 0.995 0.993 1.012 1.512 1.603 1.532 2.349 2.043 2.186 2.378 2.428 2.395 < 0.0001 0.0814
miR-219-3p 1.038 1.034 0.928 1.284 1.327 1.311 5.987 6.592 7.478 3.128 3.228 3.106 0.0013 0.0012
miR-219-5p 0.932 0.993 1.075 1.506 1.458 1.485 1.203 1.219 1.108 2.021 2.349 2.021 0.0004 0.0011
miR-22-3p 0.895 1.104 1.001 0.998 1.023 0.976 1.782 1.432 1.899 1.687 1.765 1.694 0.9879 0.9422
miR-22-5p 0.886 1.204 0.91 1.034 0.997 1.064 1.921 1.367 1.219 1.198 1.207 1.184 0.7761 0.2255
miR-221 0.889 0.994 1.117 1.257 1.389 1.347 1.342 1.453 1.440 1.302 1.302 1.200 0.0124 0.0423
miR-222 1.191 0.893 0.916 0.893 0.884 0.983 2.132 2.093 2.061 0.893 0.804 0.799 0.4719 < 0.0001
miR-223 1.033 0.994 0.973 0.534 0.603 0.583 0.823 0.753 0.778 3.872 3.338 3.877 < 0.0001 < 0.0001
miR-23a-3p 1.112 0.887 1.001 1.045 1.120 1.075 1.382 1.232 1.332 1.984 1.804 1.787 0.3078 0.0021
miR-23a-5p 1.233 0.773 0.994 0.982 0.930 0.903 0.652 0.452 0.499 0.465 0.563 0.514 0.6712 0.7756
miR-23b-3p 1.021 0.982 0.997 0.258 0.305 0.293 0.788 0.883 0.837 0.402 0.372 0.360 < 0.0001 0.0001
miR-23b-5p 0.893 0.998 1.109 0.405 0.423 0.404 2.030 2.119 1.989 0.619 0.462 0.647 0.0007 < 0.0001
miR-24-1 0.923 0.884 1.193 0.745 0.773 0.740 0.939 0.832 0.881 0.773 0.673 0.778 0.0645 0.0364
miR-24-1-5p 0.789 1.088 1.123 1.324 1.204 1.203 0.342 0.482 0.450 0.730 0.674 0.735 0.0980 0.0035
miR-24-2-5p 1.134 1.043 0.823 0.593 0.525 0.582 0.873 0.834 0.776 0.354 0.503 0.453 0.0102 0.0017
miR-25 1.209 0.884 0.907 0.398 0.337 0.383 1.293 1.092 1.180 0.775 0.745 0.749 0.0041 0.0018
miR-26a-1 1.176 0.832 0.992 0.789 0.945 0.738 4.582 4.536 4.424 1.093 1.294 1.089 0.2078 < 0.0001
miR-26a-1-3p 1.111 1.003 0.886 0.305 0.286 0.284 0.807 0.774 0.794 0.518 0.428 0.424 0.0004 0.0005
miR-26a-2-3p 0.992 0.889 1.119 1.136 1.205 1.116 1.263 1.236 1.180 1.032 1.003 1.012 0.1011 0.0012
miR-26b-5p 0.978 0.934 1.088 0.442 0.435 0.423 1.025 0.932 1.075 0.894 0.864 0.878 0.0003 0.0367
miR-26b-3p 0.834 0.998 1.168 0.523 0.672 0.556 0.542 0.438 0.347 0.328 0.784 0.338 0.0174 0.8110
miR-27a-3p 0.845 1.084 1.071 0.568 0.479 0.463 1.672 1.682 1.546 0.764 0.548 0.544 0.0041 0.0003
miR-27a-5p 0.56 1.233 1.207 1.044 1.032 1.052 0.821 0.938 0.711 1.428 1.273 1.410 0.8558 0.0026
miR-27b-3p 0.792 1.134 1.074 0.342 0.285 0.236 1.623 1.923 1.531 1.293 0.946 1.014 0.0029 0.0187
miR-27b-5p 0.899 1.045 1.056 4.123 3.895 3.935 0.048 0.432 0.165 0.894 0.893 0.793 < 0.0001 0.0055
miR-28-3p 0.993 1.003 1.004 0.884 0.795 0.883 2.784 2.332 2.258 1.328 1.295 1.324 0.0080 0.0023
miR-28-5p 1.034 0.882 1.084 0.744 0.844 0.731 1.289 1.128 1.222 1.023 1.034 1.048 0.0322 0.0197
miR-2861 1.127 0.88 0.993 0.250 0.244 0.248 4.372 4.668 4.118 0.189 0.139 0.200 0.0005 < 0.0001
miR-296-3p 0.994 0.934 1.072 1.253 1.255 1.253 0.734 0.717 0.738 1.201 1.034 1.009 0.0032 0.0044
miR-296-5p 0.873 1.026 1.101 0.684 0.625 0.639 0.572 0.600 0.574 0.342 0.336 0.387 0.0072 0.0003
miR-297a 0.805 0.994 1.201 0.376 0.391 0.381 3.203 2.773 2.951 2.884 2.339 2.870 0.0057 0.2722
miR-29a-3p 0.904 0.893 1.203 1.578 1.489 1.510 5.093 5.226 5.070 3.747 3.294 3.744 0.0075 0.0006
miR-29a-5p 1.09 0.993 0.917 0.219 0.243 0.231 0.827 0.883 0.825 0.783 0.875 0.729 0.0001 0.3501
miR-29b-1 1.188 1.004 0.808 0.673 0.665 0.662 2.371 2.982 2.383 2.103 1.904 2.026 0.0385 0.0538
miR-29b-1-5p 1.117 0.843 1.04 0.703 0.782 0.696 4.482 4.128 4.158 1.805 1.884 1.787 0.0339 < 0.0001
miR-29c-3p 1.124 0.993 0.883 0.505 0.496 0.483 2.240 2.389 2.338 1.532 1.604 1.526 0.0019 0.0001
miR-29c-5p 1.157 1.032 0.811 1.306 1.336 1.266 2.478 3.213 3.257 0.994 0.978 0.933 0.0427 0.0014
miR-300 1.021 1.034 0.945 0.457 0.420 0.444 4.328 4.582 4.564 1.123 1.245 1.106 < 0.0001 < 0.0001
miR-301a 0.993 0.889 1.118 0.198 0.157 0.175 0.923 0.819 0.805 0.548 0.492 0.496 0.0003 0.0012
miR-301b 0.924 1.032 1.044 1.003 0.894 0.984 1.275 1.138 1.149 0.917 0.837 0.842 0.4790 0.0032
miR-302a 0.893 1.123 0.984 0.903 0.993 0.903 3.746 3.336 3.335 1.128 1.284 1.297 0.4124 0.0001
miR-302b 0.995 1.193 0.812 0.238 0.243 0.228 0.652 0.558 0.549 0.628 0.664 0.638 0.0023 0.1752
miR-302c 1.094 0.893 1.013 2.634 2.468 2.501 7.682 8.172 8.310 4.889 4.927 4.976 < 0.0001 < 0.0001
miR-302d 1.034 0.872 1.094 0.667 0.568 0.640 0.231 0.273 0.223 0.184 0.175 0.161 0.0067 0.0150
miR-30a-5p 0.786 1.099 1.115 0.603 0.683 0.676 1.386 1.672 1.472 0.573 0.527 0.576 0.0348 0.0004
miR-30a-3p 0.892 1.183 0.925 0.243 0.436 0.362 1.667 1.538 1.536 0.645 0.633 0.635 0.0038 < 0.0001
miR-30b-5p 0.994 0.937 1.069 3.135 3.459 3.233 2.093 2.030 1.901 4.728 8.193 4.742 < 0.0001 0.0283
miR-30b-3p 1.093 0.782 1.125 0.794 0.883 0.802 2.070 1.983 1.813 0.993 0.892 0.996 0.1992 0.0003
miR-30c-5p 0.782 1.231 0.987 0.542 0.572 0.571 1.894 1.775 1.774 0.503 0.465 0.491 0.0281 < 0.0001
miR-30c-1-3p 0.774 1.188 1.038 1.633 1.673 1.665 0.768 0.569 0.665 0.845 0.814 0.878 0.0057 0.0418
miR-30d 1.045 0.934 1.021 0.374 0.325 0.353 1.280 1.230 1.160 0.455 0.543 0.482 < 0.0001 < 0.0001
miR-30e-5p 1.213 0.788 0.999 1.178 1.098 1.146 1.750 1.736 1.568 1.132 1.302 1.307 0.3230 0.0059
miR-30e-3p 1.024 0.878 1.098 0.757 0.746 0.759 1.645 1.535 1.457 0.639 0.589 0.641 0.0191 < 0.0001
miR-31-5p 0.562 1.231 1.207 0.778 0.805 0.797 1.239 1.021 1.017 0.719 0.992 0.988 0.3993 0.1731
miR-31-3p 0.789 1.134 1.077 0.747 0.792 0.793 1.368 1.429 1.460 1.128 1.136 1.147 0.1079 0.0005
miR-311-5p 0.893 1.092 1.015 0.782 0.840 0.887 2.347 2.226 2.295 1.034 1.042 0.967 0.0666 < 0.0001
miR-32 0.652 1.231 1.117 0.403 0.394 0.349 0.783 0.875 0.894 1.112 1.136 1.104 0.0255 0.0017
miR-323-5p 0.77 1.254 0.976 0.442 0.419 0.431 3.297 3.676 3.876 0.892 0.993 0.905 0.0154 < 0.0001
miR-324-3p 0.777 1.135 1.088 1.329 1.230 1.138 0.849 0.990 0.979 0.884 0.891 0.876 0.1369 0.2882
miR-324-5p 1.034 0.874 1.092 0.895 1.005 0.969 0.634 0.754 0.635 0.773 0.873 0.773 0.589 0.0639
miR-325 1.126 1.005 0.869 0.720 0.683 0.674 10.023 9.294 9.660 2.415 2.443 2.355 0.0152 < 0.0001
miR-326 1.032 0.845 1.123 0.535 0.569 0.525 2.998 3.004 3.091 2.135 2.242 2.362 0.0053 0.0004
miR-328 1.032 0.937 1.031 0.510 0.552 0.490 1.658 1.573 1.587 0.365 0.375 0.357 0.0002 < 0.0001
miR-329 1.177 0.992 0.831 0.483 0.506 0.501 1.231 1.433 1.135 0.720 0.728 0.710 0.0074 0.0034
miR-331-3p 1.124 0.893 0.983 1.008 0.984 0.978 0.664 0.773 0.672 0.583 0.428 0.656 0.8900 0.1239
miR-335-3p 0.994 1.005 1.001 1.386 1.518 1.483 1.563 1.446 1.471 2.034 2.132 2.155 0.0003 0.0003
miR-335-5p 0.984 1.034 0.982 1.368 1.348 1.047 1.071 1.008 1.072 0.782 0.565 0.680 0.0730 0.0048
miR-337-3p 0.789 1.088 1.123 0.649 0.598 0.642 3.256 3.445 3.320 2.104 2.303 2.076 0.0259 0.0002
miR-337-5p 1.124 0.993 0.883 0.478 0.538 0.465 2.673 2.776 2.629 1.458 1.673 1.554 0.0023 0.0001
miR-338-3p 1.287 0.783 0.93 2.603 2.587 2.523 11.324 8.788 10.854 11.283 10.739 12.749 0.0005 0.2666
miR-338-5p 0.829 0.993 1.178 0.520 0.730 0.863 12.893 13.232 13.929 4.102 4.338 3.985 0.1056 < 0.0001
miR-339-3p 0.878 1.024 1.098 1.402 1.248 1.259 2.891 1.896 2.128 1.329 1.258 1.312 0.0205 0.0289
miR-339-5p 0.979 1.167 0.854 0.603 0.598 0.788 1.223 1.413 1.314 0.738 0.775 0.769 0.0379 0.0006
miR-33b-5p 0.836 0.895 1.269 1.439 1.443 1.439 0.306 0.337 0.298 0.124 0.324 0.147 0.0314 0.1473
miR-33b-3p 1.204 0.884 0.912 1.304 1.495 1.192 1.437 1.336 1.362 0.917 0.883 0.801 0.0710 0.0004
miR-340-3p 1.106 0.888 1.006 0.583 0.556 0.548 0.562 0.633 0.494 0.538 0.243 0.587 0.0024 0.4036
miR-340-5p 1.004 0.887 1.109 0.503 0.445 0.478 6.735 6.223 6.637 4.329 4.327 4.222 0.0014 0.0002
miR-342-3p 1.009 0.893 1.098 1.407 1.309 1.350 0.997 0.878 0.753 1.502 1.443 1.459 0.0057 0.0012
miR-342-5p 1.109 0.934 0.957 0.895 1.040 0.909 1.232 1.336 1.383 1.673 1.478 1.566 0.5087 0.0238
miR-345-5p 0.542 1.178 1.28 0.231 0.229 0.267 4.656 4.568 5.263 1.890 2.093 2.110 0.0306 0.0003
miR-346 0.846 1.193 0.961 0.773 0.740 0.739 0.418 0.390 0.337 0.347 0.429 0.435 0.0721 0.5842
miR-34a 1.073 0.983 0.944 0.395 0.420 0.387 0.630 0.552 0.515 0.398 0.329 0.309 0.0001 0.0070
miR-34c-5p 0.996 0.993 1.011 0.615 0.599 0.605 0.327 0.986 0.206 0.183 0.205 0.185 < 0.0001 0.2633
miR-34c-3p 1.056 1.034 0.91 0.482 0.429 0.436 1.002 0.783 0.944 0.628 0.618 0.619 0.0003 0.0118
miR-363-3p 1.038 1.003 0.959 0.298 0.373 0.319 1.738 1.308 1.662 0.329 0.336 0.340 < 0.0001 0.0007
miR-365 0.976 0.984 1.04 1.034 1.134 1.098 1.347 1.450 1.366 1.902 1.929 1.908 0.0669 < 0.0001
miR-369-3p 0.966 1.044 0.99 0.045 0.024 0.038 0.306 0.375 0.229 0.048 0.058 0.057 < 0.0001 0.0042
miR-369-5p 1.046 0.993 0.961 0.559 0.482 0.515 1.008 1.378 1.020 0.983 1.085 0.968 0.0001 0.3859
miR-370 0.892 0.935 1.173 0.840 0.867 0.865 1.109 1.326 1.109 1.293 1.283 1.350 0.1796 0.1660
miR-371-3p 0.888 1.034 1.078 0.730 0.691 0.706 4.375 5.820 5.073 1.664 1.932 1.630 0.0076 0.0014
miR-372 0.873 0.983 1.144 0.375 0.321 0.350 1.730 1.448 1.891 0.375 0.638 0.451 0.0013 0.0014
miR-373-3p 0.984 0.799 1.217 0.462 0.477 0.461 2.678 2.884 3.007 0.718 0.755 0.781 0.0116 < 0.0001
miR-373-5p 1.093 0.884 1.023 1.285 1.382 1.316 1.784 2.074 1.982 0.665 0.587 0.553 0.0084 0.0001
miR-375 1.034 0.987 0.979 1.238 1.382 1.370 5.337 4.899 5.452 0.735 0.682 0.665 0.0026 < 0.0001
miR-376a 0.762 0.873 1.365 0.471 0.449 0.461 1.114 1.420 1.550 0.783 0.628 0.696 0.0436 0.0085
miR-376b 1.074 0.887 1.039 0.379 0.345 0.375 3.418 2.337 2.982 0.671 0.732 0.742 0.0004 0.0022
miR-376c 0.872 1.034 1.094 0.288 0.213 0.232 1.242 1.194 1.286 0.238 0.339 0.321 0.0004 < 0.0001
miR-377 0.998 1.034 0.968 1.592 1.402 1.461 3.897 4.014 4.018 2.318 1.984 2.065 0.0012 < 0.0001
miR-378-3p 0.689 1.067 1.244 0.472 0.433 0.455 1.183 1.094 1.188 0.482 0.583 0.480 0.0291 0.0002
miR-378-5p 0.865 1.134 1.001 0.342 0.330 0.323 2.639 2.459 2.514 1.128 1.098 1.150 0.0010 < 0.0001
miR-379 0.855 1.133 1.012 0.663 0.683 0.701 1.319 1.492 1.318 1.219 1.137 1.310 0.0174 0.1136
miR-380-3p 0.947 1.198 0.855 3.128 3.521 3.125 2.338 2.469 2.613 11.340 12.093 12.106 0.0002 < 0.0001
miR-380-5p 1.092 1.035 0.873 1.613 1.582 1.633 0.503 0.488 0.490 2.447 2.116 2.552 0.0008 0.0001
miR-381 1.032 0.893 1.075 0.301 0.289 0.263 1.765 1.738 1.776 0.475 0.775 0.548 0.0002 0.0002
miR-382 1.043 0.885 1.072 0.349 0.338 0.384 2.194 1.895 2.095 0.338 0.473 0.564 0.0004 0.0001
miR-409-3p 0.994 1.056 0.95 0.984 1.034 1.007 1.002 1.390 1.095 0.812 0.839 0.801 0.8183 0.0426
miR-409-5p 1.094 0.978 0.928 0.568 0.683 0.683 1.523 1.674 1.535 2.127 2.098 2.121 0.0047 0.0004
miR-410 0.945 1.034 1.021 0.542 0.535 0.525 0.183 0.227 0.213 0.124 0.092 0.143 < 0.0001 0.0112
miR-411 0.917 0.887 1.196 0.184 0.163 0.179 1.232 1.163 1.167 0.159 0.189 0.232 0.0011 < 0.0001
miR-412 0.925 0.778 1.297 3.192 3.002 2.999 3.046 3.333 3.319 2.123 2.461 2.244 0.0002 0.0022
miR-421 0.996 1.143 0.861 0.128 0.159 0.136 0.894 0.885 0.865 0.384 0.465 0.345 0.0005 0.0002
miR-422a 1.042 0.995 0.963 0.182 0.230 0.172 3.546 3.217 3.461 3.448 3.284 3.445 < 0.0001 0.8960
miR-422b 1.032 0.893 1.075 0.782 0.689 0.739 4.195 4.238 4.116 2.775 3.094 2.760 0.0126 0.0003
miR-423-3p 1.139 0.874 0.987 0.773 0.585 0.636 0.680 0.854 0.663 0.532 0.642 0.554 0.0243 0.0882
miR-423-5p 1.001 1.025 0.974 0.430 0.335 0.354 2.339 2.417 2.401 0.439 0.482 0.490 < 0.0001 < 0.0001
miR-424-3p 0.893 0.893 1.214 1.040 1.359 1.043 0.715 0.984 0.826 1.094 0.894 1.062 0.3830 0.1540
miR-425-5p 0.884 1.022 1.094 0.664 0.618 0.624 1.293 1.329 1.296 0.582 0.615 0.517 0.0045 < 0.0001
miR-425-3p 0.762 1.135 1.103 1.342 1.225 1.246 0.726 0.618 0.662 1.728 1.739 1.709 0.0954 < 0.0001
miR-429 0.692 1.235 1.073 0.995 0.894 0.997 0.129 0.158 0.175 1.893 1.946 1.965 0.8286 < 0.0001
miR-432 0.993 0.893 1.114 0.520 0.535 0.463 49.320 52.374 55.818 2.875 2.346 2.867 0.0019 < 0.0001
miR-433 1.044 0.886 1.07 0.430 0.410 0.382 6.334 6.873 6.706 0.503 0.547 0.539 0.0006 < 0.0001
miR-449a 1.049 0.873 1.078 0.694 0.782 0.700 0.953 0.887 0.900 1.028 1.038 1.021 0.0172 0.0051
miR-449b 1.121 0.788 1.091 1.134 1.120 1.062 1.638 1.634 1.622 0.719 0.673 0.729 0.3871 < 0.0001
miR-450a-5p 1.094 0.862 1.044 0.415 0.473 0.425 4.653 4.286 4.527 0.384 0.382 0.362 0.0015 < 0.0001
miR-450b-5p 0.943 0.865 1.192 0.983 0.899 0.960 2.395 2.451 2.369 1.098 1.902 1.044 0.6320 0.0192
miR-451 0.978 0.882 1.14 0.344 0.354 0.352 0.917 0.831 0.946 0.883 0.874 0.903 0.0010 0.7660
miR-452-5p 1.036 1.004 0.96 1.781 1.923 1.853 1.785 1.549 1.703 1.218 1.320 1.201 < 0.0001 0.0053
miR-454 1.032 1.135 0.833 0.395 0.420 0.371 1.329 1.354 1.345 1.372 1.985 1.737 0.0025 0.1169
miR-455-3p 0.879 1.023 1.098 0.549 0.556 0.545 0.994 0.985 0.898 1.473 1.573 1.369 0.0022 0.0015
miR-455-5p 1.049 1.099 0.852 0.083 0.092 0.079 1.000 1.328 1.087 0.984 0.643 0.565 0.0003 0.0654
miR-483-3p 1.123 1.008 0.869 0.253 0.349 0.243 8.982 7.437 8.819 0.482 0.674 0.539 0.0009 < 0.0001
miR-483-5p 1.087 1.01 0.903 0.773 0.715 0.719 6.332 6.345 6.519 1.784 1.983 1.837 0.0095 0.0001
miR-489 0.947 1.036 1.017 0.205 0.140 0.214 2.328 2.234 2.222 0.899 0.873 0.898 < 0.0001 < 0.0001
miR-492 0.977 0.779 1.244 0.291 0.234 0.240 7.430 7.519 7.356 1.784 1.938 1.954 0.0054 < 0.0001
miR-493-3p 1.043 0.945 1.012 0.567 0.549 0.576 1.049 1.038 1.050 1.232 1.438 1.178 0.0001 0.0404
miR-493-5p 0.984 0.877 1.139 2.194 2.224 2.221 1.783 1.900 1.978 4.096 3.895 4.099 < 0.0001 < 0.0001
miR-494 1.093 0.888 1.019 16.138 15.229 15.137 18.931 16.782 19.109 23.183 24.782 24.822 < 0.0001 0.0029
miR-495 0.992 1.034 0.974 0.834 0.881 0.833 0.401 0.394 0.402 0.338 0.374 0.397 0.0032 0.1662
miR-496 0.889 1.099 1.012 0.592 0.682 0.604 0.444 0.438 0.433 0.515 0.718 0.518 0.0051 0.0968
miR-497-5p 0.778 1.132 1.09 0.567 0.572 0.541 1.083 1.309 1.280 0.592 0.691 0.524 0.0172 0.0019
miR-497-3p 1.095 1.023 0.882 0.448 0.439 0.427 0.794 0.745 0.788 0.340 0.216 0.252 0.0009 0.0002
miR-498 1.128 1.004 0.868 1.020 1.003 4.000 3.228 3.574 3.565 0.972 0.874 0.979 0.3702 < 0.0001
miR-499-3p 1.093 1.05 0.857 0.238 0.281 0.259 4.291 4.457 4.540 0.917 0.703 0.712 0.0005 < 0.0001
miR-499-5p 0.983 1.004 1.013 0.293 0.301 0.289 2.105 2.136 2.156 0.805 0.872 0.805 < 0.0001 < 0.0001
miR-501-3p 0.937 0.883 1.18 1.749 1.734 1.751 1.713 1.645 1.669 1.983 1.775 1.883 0.0012 0.0318
miR-501-5p 1.134 1.12 0.746 1.213 0.834 1.237 1.849 1.845 1.854 1.836 1.884 1.929 0.6307 0.2801
miR-503 1.045 0.894 1.061 4.294 3.893 4.412 1.349 1.349 1.388 1.345 1.463 1.589 < 0.0001 0.2214
miR-504 1.031 0.883 1.086 0.442 0.350 0.513 0.329 0.342 0.152 0.221 0.302 0.205 0.0018 0.6667
miR-505-3p 0.983 0.892 1.125 1.023 1.342 1.296 1.291 1.228 1.265 0.573 0.632 0.691 0.1413 < 0.0001
miR-506 1.094 1.034 0.872 0.341 0.221 0.139 11.213 13.084 12.162 1.443 1.453 1.453 0.0010 < 0.0001
miR-508-3p 0.883 1.003 1.114 2.010 1.823 1.714 1.732 1.743 1.663 0.354 0.387 0.357 0.0015 < 0.0001
miR-508-5p 0.874 1.03 1.096 0.456 0.582 0.542 4.005 4.025 4.079 0.548 0.593 0.534 0.0033 < 0.0001
miR-512-5p 0.884 1.102 1.014 0.324 0.305 0.299 1.848 1.784 1.899 0.892 0.884 0.854 0.0004 < 0.0001
miR-518f 0.928 1.003 1.069 0.538 0.558 0.539 3.672 3.749 3.792 1.232 1.319 1.300 0.0004 < 0.0001
miR-519a-5p 0.983 1.112 0.905 0.562 0.466 0.467 3.385 3.274 3.377 1.223 1.681 0.854 0.0018 0.0016
miR-520b 1.033 1.033 0.934 3.167 3.542 2.181 31.230 32.783 31.932 46.783 48.927 51.203 0.0085 0.0002
miR-520g 1.004 0.755 1.241 0.428 0.495 0.462 2.380 2.310 2.314 1.642 1.633 1.942 0.0191 0.0046
miR-523 1.045 0.889 1.066 0.333 0.345 0.323 1.482 1.576 1.576 0.428 0.458 0.624 0.0003 0.0002
miR-524-3p 1.096 0.937 0.967 0.529 0.619 0.600 3.872 3.479 3.744 2.374 2.557 2.533 0.0017 0.0007
miR-524-5p 0.945 0.997 1.058 1.034 1.003 1.021 2.008 2.138 2.116 1.384 1.842 1.756 0.5987 0.0433
miR-526a 0.937 0.885 1.178 1.348 1.342 1.231 1.947 1.894 1.953 1.428 1.468 1.412 0.0350 < 0.0001
miR-527 1.056 0.789 1.155 1.515 1.452 1.546 2.135 2.095 2.147 1.983 1.789 1.848 0.0110 0.0133
miR-557 1.085 0.899 1.016 1.004 1.339 0.904 3.894 3.295 3.591 1.732 1.563 1.426 0.5939 0.0005
miR-572 0.994 0.782 1.224 0.345 0.420 0.377 4.339 4.782 4.915 0.342 0.439 0.331 0.0088 < 0.0001
miR-583 0.982 0.784 1.234 2.329 1.284 2.235 2.517 2.673 2.545 2.193 2.087 2.195 0.0570 0.0022
miR-584 0.847 0.883 1.27 0.402 0.443 0.399 3.226 3.872 3.812 0.894 0.884 0.894 0.0127 0.0002
miR-586 1.046 1.021 0.933 0.302 0.334 0.277 0.338 0.325 0.323 0.093 0.132 0.081 < 0.0001 0.0001
miR-602 0.983 1.223 0.794 0.883 0.520 0.832 3.229 3.418 3.275 0.993 1.093 0.915 0.2040 < 0.0001
miR-612 1.034 1.033 0.933 0.554 0.602 0.470 8.977 8.554 8.685 2.884 3.095 2.953 0.0009 < 0.0001
miR-615-3p 1.034 1.043 0.923 1.223 1.340 1.262 1.032 1.284 1.041 0.935 0.894 0.906 0.0060 0.0678
miR-615-5p 0.994 0.883 1.123 1.109 1.231 1.123 0.673 0.518 0.604 0.515 0.534 0.500 0.1236 0.1486
miR-616-3p 0.967 0.897 1.136 0.653 0.821 0.625 4.253 4.315 4.235 0.438 0.232 0.232 0.0327 < 0.0001
miR-616-5p 0.912 0.846 1.242 0.443 0.458 0.459 1.477 1.837 1.613 1.983 1.894 1.827 0.0112 0.0860
miR-623 0.919 0.876 1.205 1.023 0.834 0.835 0.983 0.684 0.694 0.745 0.774 0.689 0.4435 0.6407
miR-625 1.094 1.076 0.83 0.789 0.832 0.756 2.136 2.174 2.297 1.856 1.998 1.914 0.0776 0.0118
miR-628-3p 0.903 1.034 1.063 0.338 0.342 0.365 1.369 1.358 1.463 2.394 2.784 2.124 0.0002 0.0059
miR-628-5p 0.994 1.145 0.861 2.668 2.331 2.630 1.459 1.440 1.595 1.894 1.984 1.852 0.0003 0.0027
miR-638 1.093 1.132 0.775 0.183 0.293 0.204 3.654 3.563 3.351 0.303 0.254 0.204 0.0028 < 0.0001
miR-658 1.083 1.008 0.909 0.042 0.034 0.030 3.129 3.330 3.172 0.043 0.056 0.042 < 0.0001 < 0.0001
miR-659 1.037 1.01 0.953 0.673 0.772 0.745 4.884 4.137 4.398 1.283 1.098 1.069 0.0022 0.0001
miR-671-3p 0.903 1.005 1.092 0.203 0.320 0.199 0.340 0.371 0.355 0.321 0.232 0.289 0.0004 0.0534
miR-671-5p 1.094 0.786 1.12 0.220 0.219 0.216 0.754 0.894 0.766 0.231 0.131 0.282 0.0019 0.0007
miR-674-5p 1.034 1.13 0.836 0.440 0.482 0.467 2.385 2.481 2.340 1.319 1.273 1.039 0.0036 0.0002
miR-674-3p 0.992 1.201 0.807 1.459 1.553 1.552 45.730 42.190 47.084 40.621 38.183 43.902 0.0115 0.1369
miR-675b 1.034 1.004 0.962 0.549 0.630 0.595 5.374 5.237 5.226 0.772 0.894 0.985 0.0002 < 0.0001
miR-711 0.893 1.335 0.772 0.782 0.773 0.759 4.987 5.112 5.186 1.983 2.309 2.012 0.2527 < 0.0001
miR-758 0.782 1.112 1.106 0.384 0.556 0.284 1.444 1.528 1.438 1.239 1.113 1.152 0.0118 0.0031
miR-769-3p 1.055 1.034 0.911 1.893 1.450 1.977 4.886 3.462 4.491 1.328 1.342 1.324 0.0103 0.0023
miR-769-5p 1.066 0.878 1.056 0.814 0.773 0.800 5.447 7.226 6.111 1.714 1.775 1.723 0.0304 0.0010
miR-770-5p 1.134 0.844 1.022 1.203 1.004 1.005 1.578 1.193 1.719 0.402 0.364 0.393 0.5460 0.0021
miR-875-3p 0.811 0.84 1.349 0.308 0.225 0.284 3.427 3.385 3.357 1.643 1.917 1.982 0.0146 0.0001
miR-875-5p 0.882 0.892 1.226 0.453 0.543 0.583 0.631 0.616 0.618 0.382 0.783 0.347 0.0166 0.4478
miR-877 0.893 0.934 1.173 0.920 0.884 0.892 3.187 3.209 3.445 1.672 1.485 1.729 0.3136 0.0001
miR-9-5p 0.679 1.321 1 1.819 1.783 1.801 0.351 0.448 0.451 0.984 0.843 0.790 0.0125 0.0024
miR-9-3p 0.665 1.224 1.111 0.539 0.673 0.592 0.638 0.627 0.624 0.405 0.332 0.319 0.0850 0.0005
miR-92a 0.782 1.12 1.098 0.892 0.812 0.822 0.635 0.614 0.649 0.718 0.772 0.719 0.2313 0.0072
miR-92b 0.832 1.087 1.081 0.566 0.672 0.670 0.456 0.673 0.554 0.537 0.512 0.544 0.0161 0.6612
miR-93-5p 1.008 0.993 0.999 1.432 1.223 1.223 5.213 5.099 5.080 1.232 1.382 1.280 0.0138 < 0.0001
miR-93-3p 1.003 1.121 0.876 1.195 1.238 1.227 0.985 1.094 0.950 1.349 1.673 1.574 0.0377 0.0077
miR-95 1.104 1.036 0.86 1.332 1.320 1.291 4.883 5.209 4.956 7.873 7.674 7.695 0.0130 < 0.0001
miR-96 1.112 1.332 0.556 0.783 0.743 0.767 1.142 1.462 1.146 1.231 1.322 1.346 0.3657 0.6794
miR-98 1.093 0.892 1.015 0.784 0.772 0.731 2.438 2.459 2.431 2.336 2.873 2.856 0.0173 0.2362
miR-99a 0.776 1.232 0.992 1.805 1.743 1.701 2.113 2.094 2.188 1.923 1.883 1.927 0.0052 0.0023
miR-99b-5p 0.789 1.11 1.101 1.332 1.302 1.293 0.934 0.780 0.859 0.984 0.973 0.901 0.0437 0.1390
miR-99b-3p 0.893 1.078 1.029 1.432 1.335 1.336 0.893 0.864 0.862 1.002 1.094 1.027 0.0046 0.0045
* scramble vs. shNTSR1#1
** scramble+actD vs. shNTSR1#1+actD