Fernando Luís Cônsoli

1. Exploit insect-insect interactions

2. Exploit insect-bacterial symbiont interactions

3. Exploit insect genomic/transcriptomic information

Insect Interactions LabDepartment of Entomology and AcarologyESALQ - Universidade de São Paulo

BBSRC-FAPESP JOINT PUMP–PRIMING AWARDS for AMR and

INSECT PEST RESISTANCE IN AGRICULTURE: Understanding and

managing resistance, including novel methods, for pathogen and

pest control.

1. Isolar, identificar e caracterizar

Insect-bacterial symbiont

associations

Physiology

Development

Ecology

Behavior

Holobiome

Exploiting insect-bacterial symbiont interactions

Insecticide resistance

Xenobiotic metabolism

IIL-Lc32

Enterococcus casseliflavus (AJ420804)

IIL-Dm01

IIL-Cl05

IIL-Sp06

Enterococcus gallinarum (AJ420805)

Enterococcus durans (AJ420801)

IIL-Cl25

IIL-Lc16

IIL-Luf18

IIL-Sp24

Enterococcus mundtii (AJ420806)

Lactobacillus sp. (FN667916)

Bacillus simplex (AB363738)

Staphylococcus vitulinus (AB009946)

IIL-Luf12

Staphylococcus sciuri subsp. sciuri (AJ421446)

Actinomyces sp. (X81063)

Streptomyces sp. (AJ781371)

IIL-Dm05

Arthrobacter nicotinovorans (X80743)

Arthrobacter ureafaciens (X80744)

IIL-Cl32

Microbacterium paraoxydans (AJ491806)

Microbacterium oxydans (Y17227)

Microbacterium radiodurans (GQ329713)

Microbacterium imperiale (X77442)

IIL-Luf14

Microbacterium arborescens (X77443)

IIL-Cl22

Delftia lacustris (EU888308)

Delftia acidovorans (AF078774)

Comamonas composti (EF015884)

Diaphorobacter nitroreducens (AB064317)

IIL-Cl29

Leclercia adecarboxylata (AB273740)

Citrobacter murliniae (AF025369)

Enterobacter asburiae (AB004744)

IIL-Lc09

Pseudomonas stutzeri (CP002881)

Pseudomonas otitidis (AY953147)

Pseudomonas oleovorans (D84018)

IIL-Sp19

Pseudomonas psychrotolerans (AJ575816)

99

100

73

54

100

57

100

100

63

100

99

100

60

100

96

99

96

100

100

100

57

40

100

77

100

58

100

54

100

98

97

100

100

100

88

0.02

IIL-Lc32

Enterococcus casseliflavus (AJ420804)

IIL-Dm01

IIL-Cl05

IIL-Sp06

Enterococcus gallinarum (AJ420805)

Enterococcus durans (AJ420801)

IIL-Cl25

IIL-Lc16

IIL-Luf18

IIL-Sp24

Enterococcus mundtii (AJ420806)

Lactobacillus sp. (FN667916)

Bacillus simplex (AB363738)

Staphylococcus vitulinus (AB009946)

IIL-Luf12

Staphylococcus sciuri subsp. sciuri (AJ421446)

Actinomyces sp. (X81063)

Streptomyces sp. (AJ781371)

IIL-Dm05

Arthrobacter nicotinovorans (X80743)

Arthrobacter ureafaciens (X80744)

IIL-Cl32

Microbacterium paraoxydans (AJ491806)

Microbacterium oxydans (Y17227)

Microbacterium radiodurans (GQ329713)

Microbacterium imperiale (X77442)

IIL-Luf14

Microbacterium arborescens (X77443)

IIL-Cl22

Delftia lacustris (EU888308)

Delftia acidovorans (AF078774)

Comamonas composti (EF015884)

Diaphorobacter nitroreducens (AB064317)

IIL-Cl29

Leclercia adecarboxylata (AB273740)

Citrobacter murliniae (AF025369)

Enterobacter asburiae (AB004744)

IIL-Lc09

Pseudomonas stutzeri (CP002881)

Pseudomonas otitidis (AY953147)

Pseudomonas oleovorans (D84018)

IIL-Sp19

Pseudomonas psychrotolerans (AJ575816)

99

100

73

54

100

57

100

100

63

100

99

100

60

100

96

99

96

100

100

100

57

40

100

77

100

58

100

54

100

98

97

100

100

100

88

0.02

ChlorpyriphosLambda-cyhalothrinDeltamethrinLufenuronSpinosad

IIL-Lc32

Enterococcus casseliflavus (AJ420804)

IIL-Dm01

IIL-Cl05

IIL-Sp06

Enterococcus gallinarum (AJ420805)

Enterococcus durans (AJ420801)

IIL-Cl25

IIL-Lc16

IIL-Luf18

IIL-Sp24

Enterococcus mundtii (AJ420806)

Lactobacillus sp. (FN667916)

Bacillus simplex (AB363738)

Staphylococcus vitulinus (AB009946)

IIL-Luf12

Staphylococcus sciuri subsp. sciuri (AJ421446)

Actinomyces sp. (X81063)

Streptomyces sp. (AJ781371)

IIL-Dm05

Arthrobacter nicotinovorans (X80743)

Arthrobacter ureafaciens (X80744)

IIL-Cl32

Microbacterium paraoxydans (AJ491806)

Microbacterium oxydans (Y17227)

Microbacterium radiodurans (GQ329713)

Microbacterium imperiale (X77442)

IIL-Luf14

Microbacterium arborescens (X77443)

IIL-Cl22

Delftia lacustris (EU888308)

Delftia acidovorans (AF078774)

Comamonas composti (EF015884)

Diaphorobacter nitroreducens (AB064317)

IIL-Cl29

Leclercia adecarboxylata (AB273740)

Citrobacter murliniae (AF025369)

Enterobacter asburiae (AB004744)

IIL-Lc09

Pseudomonas stutzeri (CP002881)

Pseudomonas otitidis (AY953147)

Pseudomonas oleovorans (D84018)

IIL-Sp19

Pseudomonas psychrotolerans (AJ575816)

99

100

73

54

100

57

100

100

63

100

99

100

60

100

96

99

96

100

100

100

57

40

100

77

100

58

100

54

100

98

97

100

100

100

88

0.02

Pro

teo

bacte

ria

gamm

ab

eta

Actin

ob

acteria

Firmicu

tes

Gs1Gs2

Gs3

100%

90%

70%

20%

0%

50%

Gs4 Gs5

Pop Location

RS-09 Santo Ângelo/RS (28o17’56’’S; 54o15’46’’O)

PR-37 Sabáudia/PR (23o18’43’’S; 51o29’43’’O)

SP-14 Palmital/SP (22o46’31’’S; 50o13’46’’O)

MG-14 Uberlândia/MG (19o12’49’’S; 48o10’26’’O)

GO-18 Rio Verde/GO (17o47’52’’S; 50o55’40’’O)

BA-29 Barreiras/BA (11o40’19’’S; 36o03’40’’O)

MT-20 Campo Novo do Parecis/MT (13o24’47’’S; 57o57’51’’O)

5

3

3 2

3

SP

LUF

CHL LC

DM

Control

LC50 SUS+Ma= 25.36 µg/mL

9.2 fold increase

SUS

CTR

SUS +

Ma

LC50 SUS= 2.74 µg/mL

Exploiting insect-bacterial symbiont interactions

The role of bacterial symbionts in phenotype expression

Control

Exploiting insect-bacterial symbiont interactions

The role of bacterial symbionts in phenotype expression

Exploiting insect-bacterial symbiont interactions

The role of bacterial symbionts in phenotype expression

1. Isolar, identificar e caracterizar

Aphis citricidus – Arsenophonus – Host plant - Parasitoid

Observed parasitization (%)Ars- Ars+

Sweet orange 31.5 ± 7.43 15.0 ± 3.85

Orange jasmine 30.0 ± 9.01 12.1 ± 3.07

Mean 30.6 ± 5.7 a 13.7 ± 2.4 b

Parasitoid emergence (%)Ars- Ars+

Sweet orange 89.3 ± 7.43 -

Orange jasmine 81.3 ± 12.38 -

1. Isolar, identificar e caracterizar

Exploiting insect-bacterial symbiont interactions

Bacterial symbionts as a source of natural insecticides

2015_extrato bruto Luis_hexano

m/z200 400 600 800 1000 1200

%

0

100

%0

100

ASP 101b hex Luis 34 (0.916) Sm (Mn, 1x1.00); Cm (30:44) 1: Scan ES+ 2.66e7261.2

249.2

185.1277.2

301.1 399.3 551.4 683.5727.5 815.6

ASP 101b hex Luis 32 (0.875) Sm (Mn, 1x1.00); Cm (29:38) 2: Scan ES- 5.35e5110.7

139.6

170.7254.9

338.8 581.7

m/z200 400 600 800 1000 1200

%

0

100

%

0

100

ASP 33 (0.989) 1: Scan ES+ 6.19e71059.0136.1

261.4

261.1

250.3

1045.0

365.3

262.3 1030.9413.5

441.4 551.5 1016.9771.7

1060.0

1061.0

1076.0

ASP 33 (0.918) 4: Scan ES- 6.49e5178.7

174.7

164.7

1034.4

376.7332.7

273.8

1020.3

378.6538.7

701.0 992.3750.8

1035.5

1036.5

1056.4

m/z200 400 600 800 1000 1200

%

0

100

%

0

100

ASP 76 17 (0.975) Sm (Mn, 1x1.00); Cm (16:18) 4: Scan ES- 2.58e6160.8

144.8

96.7

404.8370.9414.9

755.9716.0

ASP 76 17 (0.975) Sm (Mn, 1x1.00); Cm (16:19) 4: Scan ES- 2.38e6160.8

144.8

96.7404.8370.9

418.8 770.0745.9

m/z200 400 600 800 1000 1200

%

0

100

%

0

100

ASP 47 25 (1.390) Sm (Mn, 1x1.00); Cm (19:26) 1: Scan ES+ 2.80e6163.0

149.0

413.3343.3

181.0

241.1522.6 550.7

551.7

563.5 732.6

ASP 47 22 (1.262) Sm (Mn, 1x1.00); Cm (20:24) 4: Scan ES- 1.86e5174.7

110.7

310.7

282.9 316.6

326.8

666.4397.5623.01163.8

1104.5736.8818.8 1290.9

m/z200 400 600 800 1000 1200

%

0

100

%

0

100

ASP 50 19 (1.046) Sm (Mn, 1x1.00); Cm (18:21) 1: Scan ES+ 1.34e8413.3147.0

149.0393.3

181.1375.3

414.4

429.3 803.7

430.3523.4708.5

804.7

805.7

ASP 50 19 (1.090) Sm (Mn, 1x1.00); Cm (15:20) 4: Scan ES- 8.21e5354.9

160.7

143.7

324.7

310.6

368.8

410.8

414.9 715.9683.8 745.9

m/z200 400 600 800 1000 1200

%0

100

%

0

100

ASP 66 17 (0.932) Sm (Mn, 1x1.00); Cm (16:19) 1: Scan ES+ 5.88e7241.2

147.1

295.2 479.3

311.2 487.5

575.5 727.6815.7 947.8

ASP 66 16 (0.918) Sm (Mn, 1x1.00); Cm (15:17) 4: Scan ES- 3.61e6228.8196.8

142.7

324.8

454.9492.8 600.9

IIL-Asp51

IIL-Asp74

IIL-Asp39

IIL-Asp64

IIL-Asp53

IIL-Asp54

IIL-Asp66

IIL-Asp70

IIL-Asp73

IIL-Asp91

IIL-Asp95

IIL-Asp97

IIL-Asp77

IIL-Asp63

IIL-Asp72

IIL-Asp67

IIL-Asp47

IIL-Asp48

IIL-Asp45

IIL-Asp68

IIL-Asp41

IIL-Asp83

IIL-Asp21

IIL-Asp82

IIL-Asp06

IIL-Asp17

IIL-Asp79

IIL-Asp20

IIL-Asp05

IIL-Asp25

IIL-Asp01

IIL-Asp36

IIL-Asp34

IIL-Asp07

IIL-Asp27

IIL-Asp32

IIL-Asp08

IIL-Asp84

IIL-Asp31

IIL-Asp88

IIL-Asp29

IIL-Asp35

IIL-Asp23

IIL-Asp87

IIL-Asp103

IIL-Asp69

IIL-Asp42

IIL-Asp09

IIL-Asp15

100

99

100

100

93

67

67

90

98

88

100

57

62

36

100

100

99

94

100

54

99

83

58

100

99

53

100

99

100

100

99

89

44

57

33

524

13

40

98

80

62

0.02

1. Isolar, identificar e caracterizar

Exploiting insect-bacterial symbiont interactions

Bacterial symbionts as a source of natural insecticides

Insecticide activity - % larval mortality

Crude

extract

Mortality

(%)

Asp38 0

Asp41 0

Asp67 0

Asp101 0

Asp82 0

Asp83 2,5 ± 1,3

Asp63 4,2 ± 6,7

Asp20 5,0 ± 2,3

Asp40 5,0 ± 1,5

Asp72 5,0 ± 1,9

Asp95 6,7 ± 3,1

Asp70 30,8 ± 5,7

Asp54 38,3 ± 7,4

Asp66 38,3 ± 8,2

Asp53 39,2 ± 9,4

Asp74 61,7 ± 6,6

Asp39 70,8 ± 8,4

Asp43 78,3 ± 9,9

Asp77 80,8 ± 7,9

3

4

5

6

7

8

9

11

12

13

14

23

10

24

18

1

25

22

17

2

21

15

16

20

19

0

0

0

0

0

0

0

0

0

0

0

5,0 ± 3,4

16,6 ± 11,2

23,3 ± 10,6

34,2 ± 10,8

35,8 ± 9,7

38,3 ± 11,3

40,0 ± 11,8

46,6 ± 8,2

54,2 ± 9,8

56,6 ± 9,8

60,8 ± 9,8

60,8 ± 10,9

75,8 ± 8,9

82,5 ± 8,4

1. Isolar, identificar e caracterizar

Exploiting insect-bacterial symbiont interactions

Bacterial symbionts as a source of natural fungicides

1. Isolar, identificar e caracterizar

Exploiting insect-bacterial symbiont interactions

Bacterial symbionts as a source of natural insecticides

1. Isolar, identificar e caracterizar Host regulation - Polydnaviridae

Bracovirus

Ichnovirus

Exploit insect-insect interaction

1. Isolar, identificar e caracterizar

Exploiting insect-insect interactions : host regulation