Instrumentation - Separationsseparations.co.za/fileadmin/themes/default/pdf/2. PRODUCT LINES/X... · 6.2 Takara Bio Europe • • [email protected] INSTRUMENTATION Thermal Cycler

6.1Takara Bio Europe • www.takara-bio.eu • [email protected]

IINSTRUMENTATION

Table of Contents

Instrumentation

Instruments

Takara PCR Thermal Cycler Dice™ …………………… 6.2

Takara PCR Thermal Cycler Dice™ Mini ……………… 6.4

PCR Tubes …………………………………………… 6.5

Mupid® One Electrophoresis Unit …………………… 6.6

Mupid® Related products …………………………… 6.6

06_EU_cat09_INSTRU_FINAL_v00.indd 1 3/11/2009 1:30:41 PM


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TaKaRa PCR Thermal Cycler Dice™ (Gradient Model) Cat.# TP600 1 unit TaKaRa PCR Thermal Cycler Dice™ (Standard Model) Cat.# TP650 1 unit

DescriptionThe TaKaRa PCR Thermal Cycler Dice™ is a GRADIENT thermal cycler (only for TP600) which allows temperature variation up to a 20°C slope between the right and left sides of the sample block in each temperature setting step. This allows optimization of reaction conditions to be performed in a single experi-ment. In addition, the use of the variable angle wing panel which combines the control panel and display makes the Dice™ compact and functional. It can be placed anywhere in the lab.

Features • Sample Setting is Easy Through use of a "Slide and Lever" Type Cover Handle.Easy Viewing and Access in Any Bench. • The control panel can be set at a desirable angle, providing:

– Easy Programming due to an excellent graphical user interface. – Simple Operation using the pop up menu and alphanumeric key input method, data entry is similar to a cell-phone.

Easy Implementation of PCR reactions• due to the eight preinstalled protocols (PCR programs).No Heat Interference Between Pieces of Equipment • due to reduced absorp-tion from the front and sides, and back gas exhaustion. Extension Time Function• suitable for long PCRExtension Temperature Function • allows touch down PCR Gradient Function (only for TP600) • enables examination of different anneal-ing temperature

Limited Use Label License: Cat.# TP600: [P10] [L18]; Cat.# TP650: [P10]

Specifications

Dimensions 260 (W) × 345 (D) × 260 (H) mm (with panel closed)

Weight 11.5 kg (System Weight)Power requirement/consumption 100-240V, 50/60Hz of AC/ 490 WHeating cooling system peltier elementRamp rate of Heating Maximum 3.0°C/sec.Ramp rate of Cooling Maximum 2.0°C/ sec.Block temperature control range 4-99.9°CBlock temperature accuracy ±0.5°CBlock temperature uniformity ±0.5°CExaggerated shot Less than 0.5°CHeated lid 110°CTemperature extension1 maximum of ±9.9°CTime extension1 maximum of ±9.59 minutesSample capacity 96 × 0.2 mL Tube or 96 well PCR Plate

DisplayBlue/white dot matrix LCD \(240 × 60 dots)

Max. programs stored 100 programsMax. user name registrations 10 users

GRADIENT function2Range which can be set up: 40–75°C (temperature variance : 6–20°C)

1 Touch down PCR2 GRADIENT is set with the first and 12th rows (right end and left end rows) of a heat block (96 wells). GRADIENT function is available only for TP600.

Application Example1. Gradient PCR Using the TaKaRa PCR Thermal Cycler Dice (TP600) System Amplification of 8-kb fragment was performed either in shuttle PCR or conven

tional PCR using λ DNA template

Reaction Mixture TaKaRa Ex Taq™ (5 units/µL) 0.25 µL 10X Ex Taq™ Buffer 5 µL dNTP Mixture (2.5 mM each) 4 µL Primer 1 (20 µM) 0.5 µL Primer 2 (20 µM) 0.5 µL λ DNA 1 µL Sterilized water 38.75 µLTotal volume 50 µL

PCR Conditions Shuttle PCR98 °C 10 sec.52 to 72 °C 5 min. (Gradient temperature : 20°C)

Conventional PCR98 °C 10 sec.45 to 65 °C 30 sec. 30 cycles(Gradient temperature: 20 °C) 5 min. 72 °C 5 min.

TaKaRa PCR Thermal Cycler Dice™

30 cycles



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Results •ResultsforShuttlePCR 2 step PCR annealing/extension temperature 52°C 72°CM (gradient) M

•ResultsforConventional PCR

3 step PCR annealing temperature 45°C 65°CM (gradient) M

2. One Step RT-PCR

The performance of the TaKaRa PCR Thermal Cycler Dice™ (Cat.# TP600) was evaluated in One Step RNA PCR by using the TaKaRa One Step RNA PCR Kit (Cat.# RR024A) and the TaKaRa PCR Thermal Cycler SP (Cat.# TP400).RT-PCR was performed in two conditions :– In the presence of AMV RTase XL in the reaction mixture.– Without AMV RTase XL in the reaction mixture.

Reaction Mixture 10X One Step RNA PCR Buffer 5 µL 25 mM MgCl2 10 µL dNTP Mixture (10 mM each) 5 µL RNase inhibitor (40 U/µL) 1 µL AMV RTase XL (5 U/µL) or DEPC treated water 1 µL AMV Optimized Taq (5 U/µL) 1 µL Control F-1 Primer 1 (20 µM) 1 µL Control R-1 Primer 2 (20 µM) 1 µL Positive Control RNA 1 µL RNase Free H2O 24 µLTotal Volume 50 µL

PCR Conditions 50°C 15 min. (RT Reaction) 94°C 2 min. (inactivation of RTase) 94°C 30 sec. 60°C 30 sec. 30 cycles72°C 1.5 min.

Results Lane Sample 1 RTase (-) 2 RTase (+) 3 RTase (-) 4 RTase (+) M 100 bp DNA Ladder

After the reaction was completed, a portion of reaction was visualized using agarose gel electrophoresis. In both cases, using TaKaRa PCR Thermal Cycler Dice™ (Cat.# TP600) and TaKaRa PCR Thermal Cycler SP (Cat.# TP400), the control reaction 462 bp product was detected only in the presence of RTase (+).

Related ProductsTaKaRa Micro PCR Tube (0.2 mL with cap) Cat.# 9047 1000 tubesTaKaRa Micro Strip PCR Tube (8 tubes per chain) Cat.# 9148 120 stripsTaKaRa Micro Strip Caps (8 caps per chain) Cat.# 9149 120 stripsTaKaRa 96 well PCR Hi-Plate (12×8 chains/plate) Cat.# NJ111 50 platesTaKaRa PCR Hi-Caps (8 caps per chain) Cat.# NJ811 120 strip

Higher annealing/extension temperatures result in reduced low-molecular nonspecific amplification and increased amplification of the target amplicon.

M : λ Hind III marker

M : λ Hind III marker

M 1 2 M 3 4

Dice (TP600) SP (TP400)



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TaKaRa PCR Thermal Cycler Dice™ Mini

Thermal Cycler Dice™ Mini Cat.# TP100 1 unit

DescriptionTakara Bio has developed the Thermal Cycler Dice series and recently added the new Dice mini to the series. The convenient Dice Mini will become a standard among personal-type 24-well block thermal cycler systems.

FeaturesThe Program Link Function• , which runs programs in numerical order if the program names are the same and “# number” is entered at the end of the names, enables a complex array of conditions to be set. Up to 22 User Names can be Registered• and up to 200 programs can be stored. Programming is Easy • due to a graphical user interface. Operation is Simple• by utilizing the pop up menu and alphanumeric key input method, the same that are similar to a cell phone. PCR Reaction can be Started Easily. • Due to the eight types of preinstalled protocols (PCR program) . No Heat Interference Between Pieces of Equipment • due to reduced absorption from the front and sides, and back gas exhaustion. Extension Time Function:• Suitable for long PCR Extension Temperature Tunction: • Allows touch down PCR Space-saving Design for Personal Use:• The Dice mini is narrower in width compared to conventional standard systems, saving space on the benchtop (TP600/TP650: 260 mm in width ; TP100: 210 mm in width).

Limited Use Label License: [P10]

Specifications

Product Name TaKaRa PCR Thermal Cycler Dice Mini

Model No. TP100Temperature accuracy +/- 0.5°C (30.0°C to 99.9°C)

+/- 2.0°C (4.0°C to 15.0°C)Temperature uniformity +/- 0.5°C (30.0°C to 99.9°C)

Temperature setting range 4.0°C to 99.9°C

Time setting range 1 second to 99 minutes, 59 seconds, ∞ (up to 9999)Heating cooling system Peltier element

Ramp rate of heating Maximum 3.0°C/sec.Ramp rate of cooling Maximum 2.0°C/sec.Tubes and plates 0.2 mL × 24 tubes

8 Strip of Tubes (0.2 mL) × 3PCR reaction volume settings 5 µL to 150 µL

Program capacity User-names: max. 22 (excluding “Common” and “TaKaRa”)Number of programs: max. 200

Lid temperature Approx. 110°C for the 20.0°C to 99.9°C rangeApprox. 40°C for the 4.0°C to 19.9°C range

Added functions Slope function, Program link•History function•Diagnosis function•PC communications (RS-232)•

(PC transmission software and R5232c cable are not provided)

Display Blue/white dot matrix LCD (240 × 60 dots)Safety devices Power switch with 5 A over-current breaker

Lid Heater temperature fuse (169°C)Thermal guard for reaction block heater plate (85°C)

Power source 100 to 240V AC, 50/60 Hz, 180VADimensions 210 (W) × 370 (D) × 260 (H) mmWeight 7.5 kg



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TaKaRa Micro PCR tube (0.2 mL with caps) Cat.# 9047 1000 tubes TaKaRa PCR Micro Strip 8-Tube Cat.# 9148 120 strips TaKaRa PCR Micro Strip 8-Cap Cat.# 9149 120 strips 0.2 mL Hi-Tube Dome Cap Cat.# NJ200 1000 tubes 0.2 mL Hi-Tube Dome Cap Recovery Cat.# NJ201 1000 tubes 0.2 mL Hi-Tube Flat Cap Cat.# NJ202 1000 tubes 0.2 mL Hi-Tube Flat Cap Recovery Cat.# NJ203 1000 tubes 0.2 mL Hi-8-Tube Cat.# NJ300 125 strips 0.2 mL Hi-8-Dome Cap Cat.# NJ301 125 strips 0.2 mL Hi-8-Flat Cap Cat.# NJ302 125 strips TaKaRa 96 well PCR Hi-Plate (12 × 8 chains/plate) Cat.# NJ111 50 plates TaKaRa PCR Hi-Caps (8 caps per chain) Cat.# NJ811 120 strips

PCR tubes

DescriptionsTaKaRa Micro PCR tube (Cat.# 9047):

Tube with attached cap. •Ideal for those applications that use only a few tubes at a time. •

TaKaRa MicroStrip PCR Tube (Cat.# 9148): Eight tube strips allows multiple PCR reactions and is ideal when using an eight •channel pipettor.Packaged 120 strips per unit.•

TaKaRa Micro Strip Caps (Cat.# 9149): 8 Cap Strips. Domed caps eliminate the need for mineral overlay when used in •thermal cyclers with heated lids.Optimized to fit the TaKaRa MycroStrip PCR Tube.•Packaged 120 strips per unit. •

TaKaRa 96 well PCR Hi-Plate (Cat.# NJ111): Robust design to minimize warp.• Raised rims minimize well-to-well contamination.•Thin wall for maximizing efficient heat transfer.•Packaged 50 plates per unit. •

TaKaRa PCR Hi-Caps (Cat.# NJ811): Eight connected caps in strip.•Flat top for comfortable application.•Optimized to fit TaKaRa 96-well PCR plates.•Packaged 120 strips per unit.•

0.2 mL Hi-Tube Dome Cap (Cat.# NJ200)0.2 mL individual PCR tubes with integral domed caps.•Dome-shaped cap for good contact with the heated thermal cycler lid. •

0.2 mL Hi-Tube Dome Cap Recovery (Cat.# NJ201)0.2 mL individual PCR tubes with integral domed caps. •Dome-shaped cap for good contact with the heated thermal cycler lid.•

0.2 mL Hi-Tube Flat Cap (Cat.# NJ202)0.2mL individual PCR tubes with integral flat caps. •Flat caps for easy closure and sample labelling.•

0.2 mL Hi-Tube Flat Cap Recovery (Cat.# NJ203)Flat caps for easy closure and sample labelling.•

0.2 mL Hi-8-Tube (Cat.# NJ300)Strips of 8 attached tubes for use with 0.2 mL strip caps.•

0.2 mL Hi-8-Dome Cap (Cat.# NJ301) Strips of 8 attached tubes for use with 0.2 mL strip tubes.•

0.2 mL Hi-8 Flat Cap (Cat.# NJ302)Strips of 8 attached caps for use with 0.2 mL strip tubes.•Flat caps for easy closure and sample labelling.•

Cat. # NJ200, NJ201, NJ202, NJ203, NJ300, NJ301 and NJ302 are DNase-free, RNase-free and pyrogen free products.


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DescriptionThe Mupid®-One electrophoresis system is CE marked. It has novel features including separated power and drain, support for multipipettes, 7 output voltage settings (18, 25, 35, 50, 70, 100, and 135V) and timer function.This product is manufactured by ADVANCE. Mupid® is a registered trademark of ADVANCE.

Features• MultiChannelPipettes:canbeusedwithmultipipettes. The Mupid®-One

allows simultaneous electrophoresis of 104 samples on a single gel (26 wells 4 rows (96 samples + 8 markers)). • The Mupid®-One is wider than the conventional Mupid® series and can support 13 or 26 lanes, enabling sample loading with multipipettors (9 mm pitch for 13 wells, 4.5 mm pitch for 26 wells).

• VoltageRange: Supports seven conventional voltages (18V, 25V, 35V, 50V, 70V, 100V and 135V). Global Input Voltage Compatibility: • variable input voltage of 100 to 240V supports use anywhere in the world Heat-Resistant Materials: • A liquid gel solution up to 100 °C can be poured into the Gel Tray. Clean up can be performed using boiling water. Lengthy Electrophoresis Possible: • wide range for voltage control which improves buffer stability during the run.

ComponentsOne gel casting stand-HR (1)* Several comb setting grooves are provided in the stand. Supports a maximum of

four combs per gel with easy position changed.One-Gel Tray L-HR (1) Accommodates up to four rows of wells (running length of 2.7 cm), running up to 104 samples (26 x 4) is possible.One-Gel Tray S-HR (2) and center partition (1) Used for making 13 and 26 sample gels.One-Comb-HR (4) Four combs can be used for L tray, whereas one comb can be used for S tray.* Number in brackets indicate number of pieces supplied with the chambers

Mupid®-One Cat.# AD160 1 set

Mupid® One

Mupid Specifications

Electrophoresis Cell Overall dimensions 183 mm (W) × 59 mm (H) × 162 mm (L) Material PPHOX Solution volume Approx. 270–320 mL (includes gel tray and gel) Multi-sample Multi-channel pippette compatible Safety Lid Overall dimensions 197 mm (W) × 38 mm (H) × 1709 mm (L) Safety Interlock system Without the lid, main power cannot be operated. Power Supply Overall dimensions 75 mm (W) × 62 mm (H) × 170 mm (L) Weight 410 g Input Voltage AC100 - 240V, (Internationally compatible) 50/60Hz Output Voltage 135V, 100V, 50V, 25V, 70V, 35V, 18V Constant peak voltage of 140V, and duty-control Timer Timer operation 0–99 min, and continuous mode Temporary shutdown supported Memory Function Automatic memory (the last used V and T) One-Gel Tray Gel tray (small) 130 mm (W) × 16.5 mm (H) × 59.5 mm (L) Gel tray (large) 130 mm (W) × 24 mm (H) × 122 mm (L) Quantity small: 2, large: 1 Material Heat Resistant Material (around 100°C of agarose available) One Comb -HR Multiple number of wells 13–26 Wells (13 wells: 9 mm spacing; 26 wells: 4.5 mm spacing) Quantity 4 combs (support making 13 or 26 wells) Material Heat Resistant Material (around 100°C of agarose available) One Gel Casting Stand -HR (makes 2 small gel trays or 1 large gel tray) Overall dimension 140 mm (W) × 20 mm (H) × 125 mm (L)

Gel Maker Set HR (Mupid® One Part #) Cat. # AD240 1 setOne Gel Tray L HR (Mupid® One Part #) Cat. # AD241 2 piecesOne Gel Tray S HR (Mupid® One Part #) Cat. # AD242 4 piecesOne Gel Maker Stand HR (Mupid® One Part #) Cat. # AD243 1 pieceOne Comb-HR (Mupid® One Part #) Cat. # AD244 2 pieces

Mupid® Related Products


Documents

Instrumentation - Separationsseparations.co.za/fileadmin/themes/default/pdf/2. PRODUCT LINES/X... · 6.2 Takara Bio Europe • • [email protected] INSTRUMENTATION Thermal Cycler