Intermediate Filaments Text and image sources are included
using the notes function of this file Cytokeratin Mitochondria
DNA
Slide 2
The IMF Family TypeProteinHuman gene #Mol WeightCell type Type
I (acidic) Type II (basic)Keratins>15 40-57 kDa 53-70
kDaEpithelia Type III (vimentin-like)Vimentin Desmin GFAP
Peripherin 11111111 57 kDa 54 kDa 50 kDa 57 kDa Mesenchymal cells
Muscle Glia, astrocytes PNS neurons Type IV (neurofilaments)NF-L
NF-M NF-H [alpha]- internexin 11111111 62 kDa 102 kDa 110 kDa 66
kDa CNS neurons CNS neurons Type V (nuclear lamina)Lamins A / C
Lamins B 1, B 2 1 1 ea 70/63 kDa 67/72 kDa Nucleus - Mature cells
Nucleus - Develop.cells * Lens (Type VI)Phakinin/CP49 Filensin1 49
kDa 95 kDaLens Others:Nestin, Synemin, Paranemin, Plasticin,
Tanabin... (Prokaryotic IMF family member)(Cresecntin)(Prokaryotes)
form heterodimers
Slide 3
Assembly as simple as abcdefg central rod domain bundles of 8
tetramers
Slide 4
Atomic Model of the Dimer
Slide 5
Keratins Lamins Cytokeratins in Epithelial Cells
Slide 6
Epidermolysis Bullosa Simplex arises from keratin point
mutations Dowling-Meara - widespread blistering Koebner -
blistering confined to hands and feet Weber-Cockayne - least
severe
Slide 7
Stretching an EBS cell destroys the keratin cytoskeleton
Slide 8
Vimentin in Fibroblasts
Slide 9
Cancer detection Cytokeratins are normally expresssed in skin
but not in the underlying tissues. Antibody staining can reveal
hyperplasia and metastasis of epithelial cells into those tissues
as a cancer progresses. Normal Skin Squalous cell carcinoma
Slide 10
Neurofilaments in Neurons NF-H in neurons GFAP in glia
Slide 11
Active Remodeling (note - short clips are repeated several
times in each part of the sequence)
Slide 12
Cytokeratin- GFP dynamics 4 hour sequence
Slide 13
Cytokeratin-GFP dynamics during mitosis 40 minute sequence
