International workshop on genotoxicity monitoring Bioassays

Piracicaba, Brazil 2006

Conducted by

Prof. Siegfried Knasmueller, Director, Institute for Cancer Research, Medical University of Vienna, Vienna, Austria

Prof. Paulo Saldiva, Department of Pathology, University of Sao Paulo, Sao Paulo, Brazil.Dr. Geraldo Stachetti-Rodrigues. Centro Nacional de Pesquisa de Monitoramento e Avaliacao

de impacio Ambiental, Brazil. Prof. Rgina Monteira, Gisela de Araga Umbuzeiro, Faculdade de Tecnologia, Universidade

Estadual de Campinas,Piracicaba, Brazil

Group photo of participants and instructors of the workshop

Prof. S. Knasmueller, Director Prof. Paulo Saldiva, Co-director

Dr. Geraldo Stachetti-Rodrigues - Instructor

Participants took a short break from the lab exercise in the hall

Professor of the University in Piracicaba giving a lecture to the participants

Instructor , S. Misik collecting inflorescences of proper stage of development for treatment

Dissecting flower bud for slide preparation

Prof. Knasmueller demonstrating the dissecting technique to a participant

Various stages of slide preparation, photographs were taken

Instructor, Miroslave Misik demonstrating Stamen hair collection for mutation studies.

Scoring stamen hair mutation events under a dissecting microscope

pink

blue

MCN

Pink mutation event in stamen-hair and microncleus (MCN) in a tetrad of Tradescantia

Scoring the frequency of micronuclei in Tradescantia tetrads

Analyzing the frequency of pink mutation events and the micronuclei frequency

Two happy participants ready for flower bud dissection with their needles.

A busy laboratory atmosphere for slide preparation

Data collection and analysis at the end of the workshop on Tradescantia assays