Introducing GenScript as our new supplierbionordika.no/application/files/1015/1488/3805/... · Reactor-Ready lab reactor features • Rapid, tool-free vessel exchange with quick-release

BioNordika AS • tel 23 03 58 00 • [email protected] • www.bionordika.no

January - March 2018

Issue 1

2

NEW SUPPLIER

Promo code NL-1/18 must be referenced when ordering. For terms see the back cover.

Introducing GenScript as our new supplier

BioNordika are happy to introduce GenScript as our newest supplier. Since November 2017 we have offered their catalog products to the Norwegian researchers.

Over the years, GenScript’s scientists have developed many innovative technologies that allow us to maintain our position at the cutting edge of biological and medical research while offering cost-effective solutions for customers to accelerate their research. GenScript offers a broad range of reagents, optimized kits, and system solutions to help you unravel the mysteries of biology.

ANTIBODIES• THE™ elite antibodies• IVD antibodies: Targeting human CEA, insulin, NGAL etc.• Epitope tag antibodies• Primary antibodies• Phosphotyrosine antibody• Secondary antibodies• Immunoassay products: Multiplexing allows for simultaneous analysis of IP etc.

PROTEINS, EXPRESSION, ISOLATION AND ANALYSIS• Protein purification resins: Excellent affinity chromatography resins for protein and antibody purification• Magnetic beads• Precast PAGE gels: Long shelf life, short running time, large loading volume, and high transfer efficiency• Protein staining: eStain L1 offers superior sensitivity compared to conventional methods and can detect protein with ultra-sensitivity and simplicity• Protein standards• Recombinant proteins

STABLE CELL LINES• Such as PD1, PD-L1, VISTA, Tim3 and Lag3

RECOMBINANT PROTEINS• Cytokines, chemokines, hormones, enzymes and inhibitors, growth factors, cytokine bundles, neurotrophines, virus antigens, CRISPR/Cas-9-related proteins, and other catalog proteins

MOLECULAR BIOLOGY REAGENT• PCR reagents• Plasmid purification• PCR cleaning• PCR cloning kit• Cloning vectors• DNA electrophoresis • Gel extraction

PEPTIDES & BIOCHEMICALS• PEDF, NT-4, CNTF, GMF-beta etc

Visit www.genscript.com for a complete overview.

ContentsGenScript . . . . . . . . . . . . . . . . . . . . . page 2

Melting point . . . . . . . . . . . . . . . . . . page 3

Rotavapor® R-100 . . . . . . . . . . . . . page 3

Mixer . . . . . . . . . . . . . . . . . . . . . . . . . . . page 3

Lab reactor . . . . . . . . . . . . . . . . . . . page 3

Media preparation . . . . . . . . . . . . page 4

Flameboy . . . . . . . . . . . . . . . . . . . . page 4

FlashGel™ system . . . . . . . . . . . . . . . . . page 5

Agaroses . . . . . . . . . . . . . . . . . . . . . . page 5

GelStar™ gel stain . . . . . . . . . . . . . . page 5

Monarch® miniprep kit. . . . . . . . . . . . page 6

DNA polymerases . . . . . . . . . . . . . . . page 6

Surface decontamination . . . . . . . page 6

NanoDrop . . . . . . . . . . . . . . . . . . . . . page 7

NGS . . . . . . . . . . . . . . . . . . . . . . . . . . . . page 8

Pippin Prep . . . . . . . . . . . . . . . . . . . . . . . . page 8

CLAM . . . . . . . . . . . . . . . . . . . . . . . . . . . . page 9

Mycoplasma detection . . . . . . . . page 10

CytoSMART™ 2 . . . . . . . . . . . . . . . . . page 10

Precast gels . . . . . . . . . . . . . . . . . . . . . . page 11

WB secondary antibodies . . . . . . page 11

Nitrocellulose sandwiches . . . . . . page 11

Antibody production . . . . . . . . . . . page 12

Immuno-oncology . . . . . . . . . . . . . . page 13

Autofluorescence . . . . . . . . . . . . . page 14

Imaging . . . . . . . . . . . . . . . . . . . . . . . . . . page 15

Multi-mode reader . . . . . . . . . . . . . page 15

Absorbance reader . . . . . . . . . . . . . . . page 15

Dilution system . . . . . . . . . . . . . . . . . page 16

Water purification . . . . . . . . . . . . . . . page 16

TOC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . page 17

FISH probes . . . . . . . . . . . . . . . . . . . . page 18

Cell-based assays . . . . . . . . . . . . . . page 18

Laboratory equipment . . . . . . . . . . page 19

GC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . page 20

Get -20%

on your first GenScript order

3Promo code NL-1/18 must be referenced when ordering. For terms see the back cover.

LABORATORY EQUIPMENT

Questions about equipment?

Contact Joachim Nedreklepp

at 922 84 963 [email protected]

The Reactor-Ready lab reactor Swap reaction vessels in minutes, not hours

Reactor-Ready is designed as a universal reactor work station with a range of easily inter-changeable vessels from 100 ml to 5 litres which can be configured to suit the chemistry and scale needed for each project. Easy to use, this one unique framework can replace many, saving money and fumehood space.

Reactor-Ready lab reactor features• Rapid, tool-free vessel exchange with quick-release vessel clamp and hose couplings• Accepts all leading brands of overhead stirrer and allows easy, tool-free adjustment• Triple support stand features heavy duty stainless steel support rods for stability• Self-aligning stirrer coupling engages without the need for tools• Innovative hose manifolds allow easy thermofluid drain down• Temperature range: -70°C to +230°C• Wide range of accessory glassware including condensers, dropping funnels etc.• Optional software allows you to log and control stirrers, circulators, balances, pumps, temperature sensors and other devices

Reaction vessel features• Range of single and vacuum jacketed vessels from 100 ml to 5 litres• Process vessels to mimic larger scale plant or manufacturing reactors• Precision engineered borosilicate glass vessels• DN100 5 neck glass lid fits all vessel sizes• Vessels feature V4 zero dead space drain stopcock which prevents over-tightening which can cause glass breakage

Rotavapor® R-100 Economical evaporation

The Rotavapor® R-100 is a high-quality rotary evaporator to meet the essential needs in classical laboratory applications. It is capable of a manually adjustable rotation speed of 20 to 280 rpm and equipped with an action jack lift for manual lifting of the evaporating flask. Included is the selected condenser, 1 L receiving and evaporating flasks and a vapor duct. All accessories are included for out of the box usage.

• Quick and ergonomic operation with manual lift mechanism• Easy flask handling and vapor duct removal with Combi-Clip • Easy operation by integrating interface, vacuum pump and recirculating chiller • Expandable with a wide range of accessories and various glass assemblies • Flexibility to use the independent heating bath for further applications

Melting point M-560 Easy and fast determination

• Designed for manual determination of melting and boiling points• Easy observation of the phase transition through a magnifying lens• Shortened measurement cycles thanks to fast heat up and cool down steps• First quick estimation possible using a temperature gradient up to 20°C/min• Parallel measurement of up to 3 samples• Intuitive calibration and verification procedures

Mixer B-400 Optimized sample preparation

• Grinds samples even with high contents of water, fat or fiber• Trace element analysis due to inert high quality ceramic knife• Microbiological testing using autoclavable materials• Resistant to low temperatures in case of frozen samples

52 500,-

69 500,-

23 000,-

4 Promo code NL-1/18 must be referenced when ordering. For terms see the back cover.

MICROBIOLOGY

Microbiological Analysis

Microbiological analysis is the use of biological, biochemical, molecular or chemical methods for the detection, identification or enumeration of micro-organisms in a material. It is often applied to disease causing and spoilage microorganisms.Microbiological analysis helps to keep under control the proliferation of viruses, bacteria, microorganism which may cause contamination, intoxication and disease. In order to achieve successful results in the isolation and identification of bacteria we should make sure we use the right water type.

Crucial step in microbiological analysis:• Sampling - Appropriate representative samples are the foundation for reliable and accurate results• Filtration - This step is important to enhance microorganism recovery and avoid exogenous contamination• Culturing - It is affected by the quality of the growth medium• Incubation - The final stage before enumerating microorganism

IMPACT OF WATERThis procedure uses samples of water and from these samples determines the concentration of bacteria.

SUITABLE PRODUCTS FROM ELGA: • CENTRA R60/R120• PURELAB Chorus 1 Complete• PURELAB Chorus 1• PURELAB Chorus 2• PURELAB flex 1 & 2• PURELAB flex 3 & 4

PRICES FROM ONLY 39 900,-

Questions about water purification or media preparation?

Monica Laukasat 404 40 960 or

[email protected]

We service your MediaclaveFOR YOUR SAFETY!

Contact Magnus Ravnås at [email protected]

Dan Nygård at [email protected]

Flameboy Handheld, portable and battery-free Bunsen burner • Piezoelectric ignition gives immediate ignition independent of any power source or battery• The handy pistol grip of Flameboy provides convenient holding and use• Various gas adapters for gas connections and -cartridges

3 450,-#145003

Mediajet vario with biplate

The Mediajet system offers the unique flexibility to fill petri dishes of various sizes, petri dishes with two compartments as well as test tubes of various diameters and length.

• Easy handling: The intuitive, full size graphical user interface makes it very easy to control all functions

• Reliable walk-away automation: Feed-In / Stack-Out technology guarantees a reliable and completely user independent operation. Production variations in the diameter or shape of the Petri dishes are easily handled by the unit, as they are actively guided throughout the entire filling process.

• Media cost reduction: A built-in “Agar Spread Function” ensures a homogenous distribution and an even surface of the agar. It optimize the agar level in the dish and allows a significant reduction of costs.

• Eliminating sources of contamination: The surface of the filling chamber is manufactured of a single piece of resistant PE, which allows convenient and efficient cleaning. Mediajet is also equipped with a UV lamp extending over the full length of the rotor where the dishes are opened during the dispensing process for optimal bactericidal efficiency.

• Biplate: For many applications, the amount of required agar can be reduced by using two-compartment Petri dishes. With the vario biplate option you have an efficient and reliable solution to fill two-compartment Petri dishes automatically.


ELECTROPHORESIS

FlashGel™ system The FlashGel™ System from Lonza gets straight to your results. Simply load samples, watch bands migrate and get data in as little as 2 minutes. Say goodbye to gel preparation, band excision, purification, and UV light. Complete separation, recovery and documentation safely, at the bench, in minutes.

• 5 minute separation and recovery• See bands in as little as 2 minutes• Real-time separation and documentation• Watch band migration as it happens• Outstanding sensitivity and resolution• Photograph gels at the bench, without DNA damaging UV light• Recover samples directly, without UV light, band excision or purification

Cat.no. Product Promo price57026 FlashGel Starter Pack Kit

Contains a FlashGel™ Dock, a pack of 1.2% DNA Cassettes, Loading dye and Marker.8 900,-

57064 FlashGel recovery kit Contains FlashGel™ Recovery Cassettes 1.2%, 8+1 double-tier well (9 pk), FlashGel™ Loading Dye, FlashGel™ Recovery Buffer, FlashGel™ QuantLadder, Visualization Glasses, and FlashGel™ Mask.

4 390,-

Questions about electrophoresis?

Contact Kristin Lundemoat 992 27 101 or

[email protected]™ nucleic acid gel stain GelStar™ nucleic acid gel stain is a highly sensitive fluorescent stain for detecting both DNA and RNA. GelStar™ stain can be added to your agarose solution prior to casting or after. Maximum sensitivity, detect as little as 20 pg of dsDNA or 3 ng of RNA.

GelStar Gel Stain, 10 000X (2 x 250 ul) Kr. 2 390,- cat.no. 50535

The world’s most trusted agarose brands Lonza is the leading innovator and world’s most trusted supplier of agarose. Our well known product brands set the standard in quality, purity and performance for electrophoresis. Lonza offers a range of agarose types designed by fragment size, sample type and application. See www.lonza.com for a complete listing.

A few of the well known Lonza agarose products are:

SEAKEM® LE AGAROSE• The standard for routine analysis • Wide resolution for most any application 100 bp - 23 kb• 25 g (#50001), 100 g (#50002), 120 g (#50000), 500 g (#50004), 1 kg (#50005)

SEAPLAQUE® AGAROSE• The original low melting agarose for recovery• Ideal for DNA and RNA recovery and cloning of tissue culture cells• 25 g (#50101), 125 g (#50100), 1 kg (#50105)

NUSIEVE® 3:1 AGAROSE• Easy to use for small fragment PCR analysis• Exceptional resolution of fragments 50 bp - 1 kb• Superior gel strength for easy handling and blotting• 25 g (#50091), 125 g (#50090), 500 g (#50094), 1 kg (#50095)

METAPHOR® AGAROSE• Resolution that rivals polyacrylamide• Resolves 2% size differences in fragments 20 bp - 800 bp• 25 g (#50181), 125 g (#50180), 500 g (#50184), 1 kg (50185)


MOLECULAR BIOLOGY

Monarch® Total RNA Miniprep KitNEB is adding a new “universal” RNA purification kit to its Monarch Nucleic Acid Purification (NAP) product portfolio. This kit can be used to purify high-quality total RNA from multiple sample types, including blood, cells, tissues, and tough-to-lyse samples (bacteria, yeast, plant, etc.).

The Monarch total RNA miniprep kit enables the purification of up to 100 μg of total RNA from a wide variety of biological samples, including blood, cultured cells and mammalian tissues. Additionally, bacteria, yeast and plant samples can be processed with additional steps to enhance lysis for these tough-to-lyse samples.

The kit includes components for DNA removal (i.e., gDNA removal columns and DNase I) and Proteinase K for digestion of tissues and blood samples. The kit also provides a reagent for the storage and preservation of samples that will not be processed immediately.

Total RNA purified using this kit ranges in size from full length rRNA to small RNA (< 200 nucleotides), including micro RNA. Purified RNA is high-quality, highly-pure and suitable for downstream applications (e.g., RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.).

Questions about molecular biology?


[email protected]

Q5® high fidelity DNA polymerase

With a fidelity >280-fold higher compared to Taq DNA polymerase and >4x higher than Phusion, Q5 is ideal for cloning and can also be used for long or difficult amplicons. Supplied with an optimized buffer system that allows robust amplification regardless of GC content.

Cat.no. Product Unit size OfferM0491 S/L Q5® High-Fidelity DNA Polymerase 100/500 units 890/3690M0493 S/L Q5® Hot Start High-Fidelity DNA Polymerase 100/500 units 990/4690

OneTaq DNA Polymerase

OneTaq DNA Polymerase is an optimized blend of Taq and Deep VentR™ DNA polymerases for use with routine and difficult PCR experiments like GC- and AT-rich PCR, colony PCR and long PCR (up to ~6 kb genomic DNA).

Cat.no. Product Unit size OfferM0480 S/L OneTaq® DNA Polymerase 200/1000 units 349/1390

RNase AWAY™ surface decontaminantEliminate RNase and DNA from laboratory surfaces

Thermo Scientific™ RNase AWAY™ is ideal for decontaminating appa-ratus, bench-tops, glassware and plasticware. It reduces dependency on carcinogenic DEPC treatments and saves time needed to bake glassware.

Cat.no. Product Unit size Offer7002 Spray bottle 475 ml 219,-7005-11 Bottle 4 L 719,-

-20%Introduction

offer

Promo code NL-1/18 must be referenced when ordering. For terms see the back cover. 7

NANODROP

Identify sample contaminants and obtain corrected concentration results with Acclaro™ Sample Intelligence technologyNanoDrop One Microvolume UV-Vis Spectrophotometers

How are the Thermo Scientific™ NanoDrop™ One Spectrophotometers different from the NanoDrop 2000/1000? NanoDrop One does everything that its predecessors can and more, including: on-board instrument control via a touch screen display, a broader dynamic range (up to 27,500 ng/ μL dsDNA), expanded connectivity and data management options, and enhanced sample analysis and technical support with Acclaro Sample Intelligence technology.

What is Acclaro Sample Intelligence technology? Thermo Scientific™ Acclaro™ Sample Intelligence technology enhances user understanding of sample quality while delivering accurate quantitative measurements. There are three parts to Acclaro: (i) data analysis algorithms that provide contaminant identification and corrected concentrations, (ii) an embedded sensor and digital image analysis that monitors the sample column for bubbles to ensure measurement integrity and (iii) sample information alerts and on-demand technical support for guided troubleshooting.

Questions about NanoDrop?


[email protected]

Original: Concentration result without any Acclaro Contaminant correction applied. Blue Spectrum

Corrected: Concentration result with Acclaro Contaminant correction applied. Green Spectrum

Impurity: Contaminant that has been detected and how much 260 nm absorbance the contaminant contributed to 260 nm peak. Orange Spectrum

Which contaminants does Acclaro detect? In DNA samples, Acclaro detects the following contaminants: proteins, phenol, Guanidine HCl and Guanidine Isothiocyanate. In protein samples (direct A280 measurements) Acclaro can detect nucleic acids and phenol. More contaminants will be offered in future software updates.

How does Acclaro help you troubleshoot? When the user taps the information icon on the measurement screen, a popup box will report the sample issue detected and offer possible causes and solutions. If the user wants to “Learn More” they will be guided through a cascade of information, from fundamentals to the specifics. This is best explained with an example. When Acclaro detects a low A260/A280 result, a popup box will inform that the ratio is outside acceptable limits for pure DNA sample and that this could be due to a poor blank or a contaminant. The user will then see information on common contaminants that can affect this ratio, and other resources starting with an animation that explains “What is a purity ratio.” If protein is the more likely contaminant, you can look at spectra of DNA/ protein samples and how increasing protein levels can affect the purity ratios of a DNA sample.


NEXT GENERATION SEQUENCING

Questions about NGS?


[email protected]

NEBNext® multiplex small RNA library prep set for Illumina®

One of the fastest growing areas of biological research is regulatory small RNA structure, processing and function. Next generation sequencing (NGS) is the method of choice for studying the variety and expression of small RNAs. The novel NEBNext® Small RNA workflow has been optimized to minimize adaptor-dimers while producing high-yield, high-diversity libraries. The NEBNext® Small RNA Library Prep Set for Illumina® includes adaptors and multiplex primers with 12 indices, to enable multi-plexing. Additional index primers for multiplexing are also available.

Each kit component must pass rigorous quality control standards, and each set of reagents is functionally validated together by construction and sequencing of indexed small RNA libraries on the Illumina sequencing platform.• Minimized adaptor-dimer contamination• High yields• Input RNA can be Total RNA• Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs

A common problem in NGS methods for small RNAs is contamination from adapter-dimer artifacts, because these artifacts are very close in size to the small RNA library elements. To address this problem, the NEBNext small RNA library prep kits use specially engineered RNA ligases, optimized workflows and novel technology to dramatically reduce the formation of adapter-dimer artifacts during library construction. Since the workflow uses total RNA as the starting material, it is beneficial to perform a final size selection step on the amplified libraries. NEB has validated size selection methods using Pippin Prep 3% gel cassettes for size selection of NEBNext Small RNA libraries.

Pippin Prep

The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross contamination.

BENEFITS OF THE PIPPIN PREP SYSTEM:• Saves time and effort compared to manual gel purification• Sample-to-sample reproducibility streamlines research• Higher sample quality improves downstream analysis

KEY DOWNSTREAM TECHNOLOGIES:• Paired-end sequencing• emPCR• RNA-seq• ChIP-seq

Nanopore sequencers give optimal results with BluePippin size selection

The Oxford Nanopore team has been speaking recently about their use of the BluePippin auto-mated size selection system for optimizing the read length obtained from nanopore sequencers. As seen with PacBio, the other long-read platform, single-molecule sequencers tend to produce reads as long as the fragments fed to them. Naturally, users interested in maximizing the read lengths of these systems want to feed them only the longest possible fragments. The simplest and most effective way to do that is with high-pass sizing, or selecting all DNA fragments longer than a certain size threshold during the sample prep process.

For the MinIon and PromethIon sequencers from Oxford Nanopore, the company recommends BluePippin sizing for various protocols. BluePippin is programmed to eliminate shorter fragments; one example of outcomes shows a whopping 255 Kb read from an E. coli experiment. There’s a similar rationale for recommending BluePippin for de novo whole genome assembly with the MinIon system.

If you’re an Oxford Nanopore customer who doesn’t already have access to one of these instruments, contact us to learn how BluePippin can make a difference in your work flow.

Fully automated sample preparation module for LCMS CLAM (Clinical Laboratory Automated sample preparation Module)

The CLAM-2000 is a fully automated sample preparation module for LCMS is based on extensive blood coagulation analyzer technology that Shimadzu has cultivated over many years. Simply place blood collection or other tubes in position and the system automatically performs everything from pretreating blood or other samples for analysis to LCMS analysis. Consequently, the system is able to minimize human error and variability in sample pretreatment procedures and reduce risk of infection. Therefore, it helps achieve a high-precision workflow for clinical research that is safer, faster, and simpler.

• Max. 350 μL sample treatment• Sample dispensing, reagent dispensing, shaking, suction filtration, and incubation• Each sample processed successively in parallel• Max. 60 samples and max. 60 filteration vials and 60 collection vials• Up to 20 bottles of reagents• LCD touch panel• Handy barcode reader • Sample and reagent cooling from 4 to 15°C (room temperature 18 to 23°C)• For connecting to an LCMS-8060/8050/8040 systems

9

SAMPLE PREPARATION


Questions about CLAM?

Contact Elisabeth Bastesonat 926 43 744 or

[email protected]

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a fully automated preparative LCMS analysis system consisting of a Nexera X2 ultra high performance liquid chromatograph, LCMS-8060 triple quadrupole mass spectrometer, and CLAM-2000 fully automated sample preparation module for LCMS.

Applications examples using CLAM-2000 combined

with LC-MS/MS

Steroids

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a

fully automated preparative LCMS analysis system consisting of a Nexera X2 ultra high performance

liquid chromatograph, LCMS-8060 triple quadrupole mass spectrometer, and CLAM-2000 fully

automated sample preparation module for LCMS.

Pretreatment Conditions and Analysis Flow

CLAM-20006 min

SamplePreparation

6 min

SamplePreparation

6 min

13 min 13 min

Sample injectionSample injectionSample injection

LC-MS/MS analysis12 min


Sample Dispensing

30 μL of serum

ReagentDispensing

60 μL of ACN with IS

Shaking

150 sec

Filtration

120 sec

SamplePreparation

6 min

Traditional Sample Preparation> 60 min

Comparison with the time required for sample preparation

Protein PrecipitationAdd ACN with IS Shake for 15 min Centrifuge for 30 min Transfer the supernatants Dry for > 30 min Reconstitute

Aldosterone

Cortisol

DHEAS

Corticosterone

11-Deoxycortisol

Androstenedione

Testosterone

17-OHP

DHEA

Progesterone

Aldosterone (neg)

DHEAS (neg)

+

+

+

+

+

+

+

+

+

+

−

−

Compounds Polarity Range (ng /mL) r2

QC sample concentrations(ng/mL)

Accuracy (%) %RSD (n=3)

LLOQ Middle ULOQ LLOQ Middle ULOQ LLOQ Middle ULOQ

0.03 – 1.14

1.51 – 320

12.9 – 2750

0.29 – 62

0.08 – 18

0.08 – 18

0.03 – 7.2

0.12 – 26

0.31 – 65

0.12 – 26.5

0.03 – 1.14

12.9 – 444

0.997

0.999

0.999

0.998

0.997

0.999

0.999

0.998

0.999

0.999

0.996

0.997

0.03

1.51

12.9

0.29

0.08

0.08

0.03

0.12

0.31

0.12

0.03

12.9

0.46

20.8

179

4.03

1.17

1.17

0.47

1.69

4.22

1.72

0.46

20.8

1.14

320

2750

62

18

18

7.2

26

65

26.5

1.14

444

96.5

104.4

104.3

101.8

103.0

116.5

93.8

103.6

93.1

103.3

115.0

101.7

102.0

100.4

101.3

101.0

102.7

99.1

101.4

103.3

98.1

101.0

97.3

103.0

102.8

98.6

99.6

98.4

96.1

100.1

100.1

99.6

99.0

99.2

97.4

99.7

6.3

1.6

3.9

4.6

5.5

4.6

3.7

6.2

6.5

3.9

6.6

2.1

7.9

0.5

3.6

0.8

0.9

1.3

1.0

1.6

3.9

1.1

7.5

1.2

3.6

0.7

1.9

1.0

0.2

1.1

1.0

1.8

6.5

1.5

8.0

7.1

Quantitative Results of Drug Spiked into Serum

For Research Use Only. Not for use in diagnostic procedures.8

Applications examples using CLAM-2000 combined

with LC-MS/MS

Steroids

This analysis flow is an example of a simultaneous multicomponent analysis of steroids in serum using a

fully automated preparative LCMS analysis system consisting of a Nexera X2 ultra high performance

liquid chromatograph, LCMS-8060 triple quadrupole mass spectrometer, and CLAM-2000 fully

automated sample preparation module for LCMS.

Pretreatment Conditions and Analysis Flow

CLAM-20006 min

SamplePreparation

6 min

SamplePreparation

6 min

13 min 13 min

Sample injectionSample injectionSample injection



Sample Dispensing

30 μL of serum

ReagentDispensing

60 μL of ACN with IS

Shaking

150 sec

Filtration

120 sec

SamplePreparation

6 min

Traditional Sample Preparation> 60 min

Comparison with the time required for sample preparation

Protein PrecipitationAdd ACN with IS Shake for 15 min Centrifuge for 30 min Transfer the supernatants Dry for > 30 min Reconstitute

Aldosterone

Cortisol

DHEAS

Corticosterone

11-Deoxycortisol

Androstenedione

Testosterone

17-OHP

DHEA

Progesterone

Aldosterone (neg)

DHEAS (neg)

+

+

+

+

+

+

+

+

+

+

−

−

Compounds Polarity Range (ng /mL) r2

QC sample concentrations(ng/mL)

Accuracy (%) %RSD (n=3)

LLOQ Middle ULOQ LLOQ Middle ULOQ LLOQ Middle ULOQ

0.03 – 1.14

1.51 – 320

12.9 – 2750

0.29 – 62

0.08 – 18

0.08 – 18

0.03 – 7.2

0.12 – 26

0.31 – 65

0.12 – 26.5

0.03 – 1.14

12.9 – 444

0.997

0.999

0.999

0.998

0.997

0.999

0.999

0.998

0.999

0.999

0.996

0.997

0.03

1.51

12.9

0.29

0.08

0.08

0.03

0.12

0.31

0.12

0.03

12.9

0.46

20.8

179

4.03

1.17

1.17

0.47

1.69

4.22

1.72

0.46

20.8

1.14

320

2750

62

18

18

7.2

26

65

26.5

1.14

444

96.5

104.4

104.3

101.8

103.0

116.5

93.8

103.6

93.1

103.3

115.0

101.7

102.0

100.4

101.3

101.0

102.7

99.1

101.4

103.3

98.1

101.0

97.3

103.0

102.8

98.6

99.6

98.4

96.1

100.1

100.1

99.6

99.0

99.2

97.4

99.7

6.3

1.6

3.9

4.6

5.5

4.6

3.7

6.2

6.5

3.9

6.6

2.1

7.9

0.5

3.6

0.8

0.9

1.3

1.0

1.6

3.9

1.1

7.5

1.2

3.6

0.7

1.9

1.0

0.2

1.1

1.0

1.8

6.5

1.5

8.0

7.1

Quantitative Results of Drug Spiked into Serum

For Research Use Only. Not for use in diagnostic procedures.8

10

CELL CULTURING


Cell Cultures

Cell culture is an important part in cellular and molecular biology, as well as within the pharmaceutical industry, as it is useful to study the effects of drugs and toxic compounds on cells. Cell culture also plays a vital part in the manufactureof viral vaccines drug screening and development. Studies into mutagenesis and carcinogenesis are also made pos-sible by this technique. Due to the critical requirement for accurate, reliable and reproducible results within these fields, cell cultures pose a major advantage for consistent results.

IMPACT OF WATERContaminated cultures and cell death are a major problem that can ultimately impact on downstream experiments. Water quality plays an important role in cell culture experimental outcomes and is used throughout the cell culture process. Not only is it the main component of buffers and media, but it is used for dissolution of additives and drugs etc.Apyrogenic ultrapure water (Type I) should be used for the preparation of media and buffers to ensure that cells are free from bacterial, yeast and viral con-taminants. Contamination of cell culture by metal ions, endotoxins, bacteria or fungi can impact the culture directly or indirectly (e.g. changes in pH).

SUITABLE PRODUCTS FROM ELGA: • CENTRA R200• PURELAB Chorus 1• PURELAB Chorus 2+• PURELAB flex 3 & 4


Mycoplasma detectionResults in < 20 min

One of the most common contaminants present in cell culture laboratories are mycoplasma. A conservative estimate states that between 15 - 35% of all continuous cell cultures are contaminated with mycoplasma. Mycoplas-ma grow slowly and do not kill the cells outright but affect various cellular parameters. Thus, mycoplasma contaminations can seriously impact the reliability, reproducibility, and consistency of experimental results.

Lonza provides a powerful product offering for reliable detection and successful elimination and prevention of mycoplasma contaminations:

• DETECTION Easily detect mycoplasma using the MycoAlert™ Mycoplasma Detection Kits with a luminometer• ELIMINATION Efficiently eliminate mycoplasma with the MycoZap™ Elimination Reagent• PREVENTION Reliably prevent mycoplasma with MycoZap™ Antibiotics or MycoZap™ Spray

Questions about cell culturing?


[email protected]

CytoSMART™ 2

The CytoSMART™ 2 System is designed for bright-field live cell imaging for applications, such as general cell culture monitoring, documentation and scratch/migration assays, which require a larger field of view. The field of view is 2.4 x 1.4 mm and the magnification is similar to that typically achieved with a 10X objective using a conventional microscope.

Conveniently sized to fit inside any incubator and with a more powerful camera, the new CytoSMART™ 2 System makes live cell imaging effortless. Images captured can be transmitted to The Cloud, enabling you to view your cultures at any time and from anywhere, without having to step into the laboratory.

57 500,- Introduction

offer!

on all MycoAlert products

-15%


WESTERN BLOTTING

Precast gels High resolution & Robust separation

GenScript's ExpressPlus™ gels are high quality precast polyacrylamide gels that are designed to separate a wide range of protein sizes by electro-phoresis. It's Bis-Tris formulation helps to preserve protein integrity and enhances the shelf life of the gels.

Features• Clean separation generates sharp, even-distributed protein bands• Consistent performance from gel to gel• Bis-Tris gel formula minimizes protein modifications and delays acrylamide hydrolysis• Easy-to-load well design with alternatives for 40, 60 and 80 ml• Available % acrylamide: 8%, 10%, 12%, 4-12%, 4-20%, 8-16%• Fits most major brand mini tanks• No special loading tips required• Up to 1 year shelf life at 2-8 °C• MOPS buffer are included

-20%Introduction

offer

% acrylamide Wells no. Well volume Quantity Cat.no. Pack price offer Price/gel8% 10, 12, 15 80, 60, 40 ml 20 gels M00810, M00812, M00815 1820,- 91,-10% 10, 12, 15 80, 60, 40 ml 20 gels M01010, M01012, M01015 1820,- 91,-12% 10, 12, 15 80, 60, 40 ml 20 gels M01210, M01212, M01215 1820,- 91,-4-12% 10, 12, 15 80, 60, 40 ml 20 gels M42010, M42012, M42015 1820,- 91,-4-20% 10, 12, 15 80, 60, 40 ml 20 gels M81610, M81612, M81615 1820,- 91,-8-16% 10, 12, 15 80, 60, 40 ml 20 gels M41210, M41212, M41215 1820,- 91,-

Western blotting secondary antibodies

Nitrocellulose sandwiches

Nitrocellulose sandwiches from Cell Signaling Technology® are pre-packaged sandwiches consisting of a precut nitrocellulose membrane and 2 sheets of 3 mm thick blotting paper. Intended to provide convenience and time savings, they are also validated for use in CST's Western Blot protocol and are the preferred membrane used by Cell Signaling Technology scientists.

Nitrocellulose sandwiches are ideal for binding proteins of all molecular weights; the 0.2 μm pore size ensures high retention of low molecular weight proteins, even below 20 kDa. Nitrocellulose sandwiches are suitable for most common transfer methods including tank, semi-dry, and vacuum blotting. This product is also suitable for colorimetric, fluorescent, and chemiluminescent detection. For chemiluminescent detection, CST recommends HRP based systems. Unlike PVDF, nitrocellulose does not require a methanol pre-wetting step.

Nitrocellulose sandwiches, 10 packKr. 1 984,- cat.no. 12369P2

Cat. no. Product OfferHRP CONJUGATED SECONDARY ANTIBODIES7075S Anti-biotin, HRP-linked antibody, 1 ml 1750,-5571S Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate), 1 ml 1707,-46387S Anti-Blue (2D2F11) Mouse mAb (HRP Conjugate),

100 ul2774,-

7076S Anti-mouse IgG, HRP-linked Antibody, 1 ml 1750,-7074S Anti-rabbit IgG, HRP-linked Antibody, 1 ml 1750,-7077S Anti-rat IgG, HRP-linked Antibody, 1 ml 1750,-5127S Mouse Anti-rabbit IgG (Conformation Specific)

(L27A9) mAb (HRP Conjugate), 100 ul2240,-

58802S Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate)

2240,-

BIOTINYLATED SECONDARY ANTIBODIES14709S Anti-mouse IgG (H+L), Biotinylated Antibody, 1 ml 1846,-14708S Anti-rabbit IgG (H+L), Biotinylated Antibody, 1 ml 1846,-ALKALINE PHOSPHATASE CONJUGATED SECONDARY ANTIBODIES7055S Anti-biotin, AP-linked Antibody, 1 ml 1750,-7056S Anti-mouse IgG, AP-linked Antibody, 1 ml

12

ANTIBODY PRODUCTION

Custom polyclonal antibody - Speedy 28-DayYour polyclonals in just 28 days!

Speedy 28-Day is the reference program for the production of custom polyclonal antibodies at Eurogentec. This proprietary program uses a non-Freund adjuvant that stimulates the immune response and gives mostly IgG’s, despite the short immunization time. Since 2007, their exclusive Speedy 28-Day program has allowed the production of >6000 polyclonals.

SPEEDY ANTI-PEPTIDE PROGRAMSThis program includes: peptide design and synthesis, coupling to a carrier protein, 28-Day immunization, and an ELISA guarantee. The Speedy anti-peptide program has an average yield of 10 - 20 mg specific antibody per rabbit and can be performed in rabbits, rats, guinea pigs and goats. Eurogentec has been designing peptides for immunizations for over 20 years and have developed specific algorithms for the most accurate design of good antigens. As a result their success rate is >95%.

SPEEDY DXTo increase the chance of success the Speedy-DX program uses two peptides, corresponding to spatially distinct regions of the target protein surface. This increases the opportunity of the resultant polyclonals to recognise the target protein in its natural environment.

SPEEDY POST TRANSLATIONAL MODIFICATION (PTM)The Speedy 28-Day includes a very efficient immunization protocol with frequent boosts. It is therefore well-adapted to generate Abs, specifically targeting PTMs, as it can minimize instability of the modification(s) on the peptide used as antigen. By far the most popular requested PTMs specific Abs are directed to phosphorylated residues but they can also do anti-methylation, anti-acetylation and many more PTM Abs.

www.anaspec.com | www.eurogentec.com

Speedy 28-Day is the reference program for the production of

custom polyclonal antibodies at Eurogentec.

This proprietary program uses a non-Freund adjuvant that

stimulates the immune response and gives mostly IgG’s, despite

the short immunization time.Since 2007, our exclusive Speedy 28-Day program has allowed the production of >6000 polyclonals.

To start, you can choose to use your own antigen or we can synthesize

peptide(s) for immunization.

Speedy anti-peptide programsThis program includes: peptide design and synthesis at Eurogentec, coupling to a carrier protein, 28-Day immunization, and an ELISA guarantee (please see the table for more information).The Speedy anti-peptide program has an average yield of 10-20 mg specific antibody per rabbit and can be performed in rabbits, rats, guinea-pigs and goats.We have been designing peptides for immunizations for over 20 years and have developed specific algorithms for the most accurate design of good antigens. As a result our success rate is >95%.

Speedy DxTo increase the chance of success the Speedy-DX program uses two peptides, corresponding to spatially distinct regions of the target protein surface. This increases the opportunity of the resultant polyclonals to recognise the target protein in its natural environment.

Speedy Post Translational Modification (PTM)The Speedy 28-Day includes a very efficient immunization protocol with frequent boosts. It is therefore well-adapted to generate Abs, specifically targeting PTMs, as it can minimize instability of the modification(s) on the peptide used as antigen.By far the most popular requested PTMs specific Abs are directed to phosphorylated residues but we can also do Anti-Methylation, Anti-Acetylation and many more PTM Abs.

Speedy MiniThe Speedy Mini is designed to give you quick access to a small quantity of specific custom antibody, purified and hence ready-to-use. The Speedy Mini package includes the peptide design and production by Eurogentec, the 28-Day immunization of one Rabbit, the purification of 10 mL of serum, the delivery of the remaining serum, and an ELISA guarantee (see table for more information).

SPEEDY 28-DAY PROTOCOL Pre-immune bleed Medium bleed Final bleed0 7 10 18 21 28 Day

Bleed

Injection

Life

Sci

ence

s

Custom Polyclonal Antibody

Speedy 28-Day Your Polyclonals in just 28 days!

SPEEDY 28-DAY SPEEDY PTMAntigen alternative 1 =

Peptide/Protein sent by the Customer

Antigen alternative 2 =Peptide produced by Eurogentec

1 or 2 unmodified

1 modified (PTM) and 1 unmodified

Peptide Design Peptide Synthesis >70%, 10-16 aa, 15-25 mg >70%, 10-13 aa, 15-20 mg (modified),

10-20 mg (unmodified)Peptide Coupling to carrier

Coupling of 5 mg peptide to carrier

Coupling of 5 mg peptide to carrierCarrier: KLH, BSA, OVA, or THY

Available hosts: rabbit, rat, guinea pig or goat

Affinity Purification + ELISA Optional Double Purification of 50 ml Serum, ELISA

OTHER PROGRAMS AND OPTIONSIn addition to its trusted Speedy 28-Day immunization programs, Eurogentec develops polyclonal antibodies using classical methods, such as 3 month-programs. They also generate monoclonal antibodies from hybridomas. For over a decade, Eurogentec exclusively produces mAbs in vitro with the best production scheme selected according to the requested antibody amount and the clone productivity. Additional Services including but not limited to antibody purification, labeling, coupling to magnetic beads and ELISA are available on request.

Questions about antibody production?

Contact Målfrid Røe



Prices from

13 850,-Special offer!


IMMUNO-ONCOLOGY

Immuno-oncologyAntibody tools for your research

PD-1PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) #84651

Background: The programmed cell death 1 protein (PD-1, PDCD1, CD279) is a member of the CD28 family of immunoreceptors that regulate T cell activation and immune responses. The PD-1 protein contains an extracellular Ig V domain, a transmembrane domain, and a cytoplasmic tail that includes an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an immunoreceptor tyrosine-based switch motif (ITSM). PD-1 is activated by the cell surface ligands PD-L1 and PD-L2. Upon activation, PD-1 ITIM and ITSM phosphorylation leads to the recruitment of the protein tyrosine phosphatases SHP-1 and SHP-2, which suppress TCR signaling. In addition to activated T-cells, PD-1 is expressed in activated B-cells and monocytes, although its function in these cell types has not been fully characterized (8). The PD-1 pathway plays an important role in immune tolerance; however, research studies show that cancer cells often adopt this pathway to escape immune surveillance. Consequently, blockade of PD-1 and its ligands is proving to be a sound strategy for neoplastic intervention.

Applications: W, IP, IHC-P, IF-IC, IF-FSpecies Cross-Reactivity (determined by WB): M, (R, Hm)Isotype: Rabbit IgG

OX40LOX40L (D6K7R) Rabbit mAb (IHC Specific) #59036

Background: OX40 (TNFRSF4, CD134) is a member of the tumor necrosis factor (TNF) receptor super-family that regulates T cell activity and immune responses. The OX40 protein contains four cysteine rich domains, a transmembrane domain, and a cytoplasmic tail containing a QEE motif. OX40 is primarily expressed on activated CD4+ and CD8+ T-cells, while the OX40 ligand (OX40L, TNFSF4, CD252) is predominantly expressed on activated antigen presenting cells. The engagement of OX40 with OX40L leads to the recruitment of TNF receptor-associated factors (TRAFs) and results in the formation of a TCR-independent signaling complex. One component of this complex, PKCθ, activates the NF-κB pathway. OX40 signaling through Akt can also enhance TCR signaling directly. Research studies indicate that the OX40L-OX40 pathway is associated with inflammation and auto-immune diseases. Additional research studies show that OX40 agonists augment.

Applications: IHC-PSpecies Cross-Reactivity (determined by WB): HIsotype: Rabbit IgG

PVR/CD155PVR/CD155 (D8A5G) Rabbit mAb #81254

Background: Poliovirus receptor (PVR, CD155) is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. Also known as nectin-like 5 (Necl-5), PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge ofmigrating cells. Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells. Additional research indicates that PVR (CD155, Necl-5) expression may play a role in invasiveness of lung adenocarcinoma. In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity.

Applications: W, IHC-PSpecies Cross-Reactivity (determined by WB): HIsotype: Rabbit IgG

ALSO AVAILABLE: Antibodies against Galectin-9 and F4780.

Thank you for your recent purchase. If you would like to provide a review visit cellsignal.com/comments.

www.cellsignal.com© 2017 Cell Signaling Technology, Inc.

EL4 NIH/3T3

Immunohistochemical analysis of paraffin-embedded mouse colon using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse ovary using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse lung using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Confocal immunofluorescent analysis of EL4 cells (positive, left) and NIH/3T3 cells (negative, right) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Note: PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) has been validated on freshly-prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminshes the performance of this antibody.



EL4 NIH/3T3

Immunohistochemical analysis of paraffin-embedded mouse colon using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse ovary using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse lung using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Immunohistochemical analysis of paraffin-embedded 4T1 mammary tumor using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific).

Confocal immunofluorescent analysis of EL4 cells (positive, left) and NIH/3T3 cells (negative, right) using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of mouse spleen using PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Note: PD-1 (D7D5W) XP® Rabbit mAb (Mouse Specific) has been validated on freshly-prepared frozen tissue sections that were fixed for 15 minutes in 4% formaldehyde. Over-fixation diminshes the performance of this antibody.

Confocal IF analysis of

EL4 cells using PD-1 (D7D5W)

XP® Rabbit mAb (Mouse Specific)

(green). Blue pseudocolor:

DRAQ5® #4084 (fluorescent

DNA dye).

IHC analysis of paraffin-embedded

mouse ovary using PD-1 (D7D5W) XP®

Rabbit mAb (Mouse Specific).


human coloncarcinoma using OX40L

(D6K7R) Rabbit mAb (IHC Specific).



Immunohistochemical analysis of paraffin-embedded A549 cell pellet (left, positive) and Daudi cell pellet (right, negative) using PVR/CD155 (D8A5G) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using PVR/CD155 (D8A5G) Rabbit mAb.

Applications: W—Western IP—Immunoprecipitation IHC—Immunohistochemistry ChIP—Chromatin Immunoprecipitation IF—Immunofluorescence F—Flow cytometry E-P—ELISA-Peptide Species Cross-Reactivity: H—human M—mouse R—rat Hm—hamster Mk—monkey Mi—mink C—chicken Dm—D. melanogaster X—Xenopus Z—zebrafish B—bovine Dg—dog Pg—pig Sc—S. cerevisiae Ce—C. elegans Hr—Horse All—all species expected Species enclosed in parentheses are predicted to react based on 100% homology.

For Research Use Only. Not For Use In Diagnostic Procedures.

Support: +1-978-867-2388 (U.S.) www.cellsignal.com/support

Orders: 877-616-2355 (U.S.)

[email protected]



Applications Species Cross-Reactivity* Molecular Wt. Isotype

IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.

W, IHC-P H 60-80 kDa Rabbit IgG**Endogenous

Background: Poliovirus receptor (PVR, CD155) is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. Also known as nectin-like 5 (Necl-5), PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells (1,2). Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells (3). Additional research indicates that PVR (CD155, Necl-5) expression may play a role in invasiveness of lung adenocarcinoma (4,5). In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity (6).

Specificity/Sensitivity: PVR/CD155 (D8A5G) Rabbit mAb recognizes endogenous levels of total CD155 protein.

Source/Purification: Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn188 of human CD155 protein.

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

*Species cross-reactivity is determined by western blot.

** Anti-rabbit secondary antibodies must be used to detect this antibody.

Recommended Antibody Dilutions: Western blotting 1:1000 Immunohistochemistry (Paraffin) 1:200† Unmasking buffer: SignalStain® Citrate Unmasking Solution

(10X) #14746 Antibody diluent: SignalStain® Antibody Diluent #8112 Detection reagent: SignalStain® Boost (HRP, Rabbit) #8114 †Optimal IHC dilutions determined using SignalStain® Boost IHC Detection Reagent.

For product specific protocols and a complete listing of recommended companion products please see the product web page at www.cellsignal.com

Background References: (1) Takai, Y. et al. (2008) Nat Rev Mol Cell Biol 9, 603-15.

(2) Sakisaka, T. et al. (2007) Curr Opin Cell Biol 19, 593-602.

(3) Minami, Y. et al. (2007) Biochem Biophys Res Commun 352, 856-60.

(4) Tane, S. et al. (2013) Exp Mol Pathol 94, 330-5.

(5) Nakai, R. et al. (2010) Cancer Sci 101, 1326-30.

(6) Stanietsky, N. and Mandelboim, O. (2010) FEBS Lett 584, 4895-900.

Tween is a registered trademark of ICI Americas, Inc.

PVR/CD155 (D8A5G) Rabbit mAb

rev. 06/08/17#812

54

UniProt ID #P15151Entrez-Gene ID #5817

Stor

e at

-2

0°C

Western blot analysis of extracts from various cell lines using PVR/CD155 (D8A5G) Rabbit mAb (upper) and β-actin (D6A8) Rabbit mAb #8457 (lower).

SignalStain and Cell Signaling Technology are trademarks of Cell Signaling Technology, Inc.

kDa

PVR/CD155

β-Actin

SNB19

200

140

100

80

6050

40

30

40

30

9

20

HT-108

0

HUVEC

A549

Daudi

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using PVR/CD155 (D8A5G) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using PVR/CD155 (D8A5G) Rabbit mAb.

WB analysis of extracts from

various cell lines using PVR/CD155

(D8A5G) Rabbit mAb (upper) and β-actin (D6A8) Rabbit

mAb#8457 (lower).


human prostate carcinoma using PVR/

CD155 (D8A5G) Rabbit mAb.


IMMUNOCHEMISTRY


Immunochemistry

IMMUNOCYTOCHEMISTRYImmunocytochemistry (ICC) is theprocess of visualizing specific proteins orantigens and the interaction of binding antibodies in whole and intact cells. These studies allow researchers to evaluate whether cells are expressing specific antigens. IMMUNOHISTOCHEMISTRYImmunohistochemistry (IHC) refers to the process of localizing antigens in tissue cells, exploiting the principle of antibodies binding specifically to antigens in biological tissues. It is a particularly important process in the diagnosis of malignant cells such as cancerous tumours. Water is required across the histologies labs, from water and float-ation baths, as well as being in most of the automated instruments present.

IMPACT OF WATERAs water is required across the field of immunochemistry, it is important that you have the right water type for your requirements. Hard deposits or bacterial biofilms can build up if poor water quality is used. When water comes into direct contact with the samples, it is important that there are no impurities or contaminants which could interfere with the antibodies and assays.

SUITABLE PRODUCTS FROM ELGA: • CENTRA R200• CENTRA R60/R120• PURELAB Chorus 1 Complete• PURELAB Chorus 1• PURELAB Classic• PURELAB flex 1 & 2• PURELAB flex 3 & 4


TrueVIEW™ autofluorescence quenching kitDramatically reduce tissue autofluorescence Reveal true immunofluorescence – even in challenging tissues Vector® TrueVIEW™ Autofluorescence Quenching Kit provides a novel way to remove unwanted fluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. This unique formulation binds and effectively quenches the auto-fluorescent elements in even the most problematic tissues, such as kidney, spleen and pancreas. The use of Vector® TrueVIEW™ Quenching reagent leads to significant enhancement in overall signal-to-noise in most immuno-fluorescence assays. Unmatched effectivenessMost methods for reduction of tissue autofluorescence act primarily onlipofuscin granules, and are not broadly effective against the most commonsources of autofluorescence targeted by Vector® TrueVIEW™ QuenchingReagent. Vector® TrueVIEW™ Quenching reagent is a unique approach to diminishunwanted autofluorescence from non-lipofuscin sources, that retains thespecific fluorescent antigen staining. The quenching action of the kit reagents therefore, provides the investigator with a clear, unambiguous, “true view” visualization of the intended target.

Cat.no. SP-8400: Vector® TrueVIEW™ Autofluorescence Quenching Kit

Reveal true immunofluorescence – even in challenging tissues

Vector® TrueVIEW™ Autofluorescence Quenching Kit provides a novel way to remove unwanted fluorescence in tissue sections due to aldehyde fixation, red blood cells, and structural elements such as collagen and elastin. This unique formulation binds and effectively quenches the autofluorescent elements in even the most problematic tissues, such as kidney, spleen and pancreas.

The use of Vector® TrueVIEW™ Quenching reagent leads to significant enhancement in overall signal-to-noise in most immunofluorescence assays.

Unmatched effectiveness

Most methods for reduction of tissue autofluorescence act primarily on lipofuscin granules, and are not broadly effective against the most common sources of autofluorescence targeted by Vector® TrueVIEW™ Quenching Reagent.

Vector® TrueVIEW™ Quenching reagent is a unique approach to diminish unwanted autofluorescence from non-lipofuscin sources, that retains the specific fluorescent antigen staining. The quenching action of the kit reagents therefore, provides the investigator with a clear, unambiguous, “true view” visualization of the intended target.

vectorlabs.com

Vector® TrueVIEW™ Autofluorescence Quenching KitDramatically Reduce Tissue Autofluorescence

WITH TrueVIEW™ QuencherWITHOUT Treatment

Adjacent human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor® Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section (right) with retention of well-defined, specific signal in both red and green channels.

• Specific reduction of autofluorescence from aldehyde fixation

• Improved signal-to-noise ratio

• Effective in even the most challenging tissues

• Easy-to-use, one-step method

• Quick 5 min incubation

• Compatible with a wide selection of fluorophores

• Compatible with standard epifluorescence and confocal laser microscopes

Why TrueVIEW™ Quencher?

Patent pending formulation.

WITHOUT Treatment WITH TrueVIEW™ Quencher

Adjacent human spleen sections (FFPE) stained using mouse anti-CD20 (red) and rabbit anti-Ki67 (green) primary antibodies detected with VectaFluor® Duet kit (DK-8818). Note significant reduction of autofluorescence in the treated section (right) with retention of well-defined, specific signal in both red and green channels.

Questions about Vector products?


[email protected]

Features• Specific reduction of autofluorescence from aldehyde fixation• Improved signal-to-noise ratio• Effective in even the most challenging tissues• Easy-to-use, one-step method• Quick 5 min incubation• Compatible with a wide selection of fluorophores• Compatible with standard epifluorescence and confocal laser microscopes

IMAGING & MICROSCOPY


WITH TrueVIEW™ Quencher

Imaging and microscopy

BioTek’s unique approach to imaging and microscopy requirements offers a range of cell imaging systems designed to suit many applications from simple, fixed cell assays and slide scanning to advanced, environmentally controlled long term live cell kinetic assays and 3D spheroid formation imaging.

Imaging & plate reader questions?

Contact Joachim Nedreklepp


800 TS absorbance reader• High quality, high performance at an affordable price• Application versatility: ELISA, protein and other endpoint assays to kinetics and cell based assays• Color touchscreen for quick, easy programming and operation• USB flash drive for convenient data export, Gen5 import for analysis• High precision and accuracy for quality results

NEW!

Cost-effective hybrid multi-mode reader Value, sensitivity, flexibility

• Patented Hybrid Technology™ combines flexible monochromator detection with high performance dichroic-based detection• Compatible with the Gas Controller for control and monitoring of CO2 and O2• Compatible with Take3™ Micro-Volume Plate: samples down to 2 µL volume can be measured. Especially useful when working with precious samples, for fast and accurate DNA/RNA quantification at 260 nm• A third generation quadruple grating monochromator for maximum flexibility and ease of use, with a 1 nm step• Dichroic-based filter optics, for best performance and advanced detection technologies such as fluorescence polarization and time resolved fluorescence• Comes with Gen5 software: reader control, advanced data analysis and flexible Excel export in one software package• Modular and upgradable, including dispenser and isstacker compatible• Detection modes: UV-Vis absorbance, fluorescence intensity, luminescence, fluorescence polarization, time-resolved fluorescence

Prices from

280 000,-w/monochromator,

incl. software

3D cell cultureApoptosisCell countingCell Migration/InvasionCell ProliferationCell Viability/ToxicityConfluenceGenotoxicityGPCR second messengersHistologyImmunofluorescenceLabel-free cell countingLive cell imagingPhenotypic assaysStem cell differentiationTransfection efficiency

16

SERIAL DILUTION • WATER PURIFICATION

Promo code NL-1/18 must be referenced when ordering. For terms see the back cover. Promo code NL-1/18 must be referenced when ordering. For terms see the back cover.

Questions?

Contact Monica Laukas


Dilucup™ serial dilution system Saves time and money spent on preparing your own dilution vials

Easy as 1, 2, 3!1. Simply add 1 ml sample to the first Dilucup2. Start the shaker by waving your hand in front of the motion sensor. When the dilushaker stops, the next diltuion is ready3. With a new pipet tip, collect 1 ml from the first cup and release it into the second cup and start the shaker again. Continue this until the final dilution is reached.

Dilucup Elegance MRD (Maximum Recovery Diluent)40DEMRD3 Max Recovery Diluent, dilution blank, 9 ml fill, ready-to-

use cups, 672 (2 x 16 x 21) total cups with 3 cups/row40DEMRD6 Max Recovery Diluent, dilution blank, 9 ml fill, ready-to-

use cups, 672 (2 x 8 x 42) total cups with 6 cups/rowDilucup Elegance BPW (Buffered Peptone Water)40DEBPW3 Buffered Peptone Water, dilution blank, 9 ml fill, ready-

to-use cups, 672 (2 x 16 x 21) total cups with 3 cups/row40DEBPW6 Buffered Peptone Water, dilution blank, 9 ml fill, ready-

to-use cups, 672 (2 x 8 x 42) total cups with 6 cups/rowDilucup Elegance NaCl, 0.85%40DENACL3 NaCl with buffer, dilution blank, 9 ml fill, ready-to-use

cups, 672 (2 x 16 x 21) total cups with 3 cups/row40DENACL6 NaCl with buffer, dilution blank, 9 ml fill, ready-to-use

cups, 672 (2 x 8 x 42) total cups with 6 cups/row

Reliable and efficient water purification When you need to ensure that your clinical labs run to their full potential with no downtime, we know that you need ultrapure water 24/7. A constant and reliable supply of compliant water is not only needed to attain accurate and reproducible results, it is critical.

Working in partnership with major clinical diagnostics companies, we have designed our unrivalled MEDICA range. The technology used, produces the highest CLRW standard water and results in predictable consumable and running costs.

The MEDICA Pro range is specifically designed to meet the quality needs of high performance, high volume single or multiple Clinical Diagnostic Analyzers to Clinical Laboratory Reagent Water (CLRW) standard. All the systems deliver 4 l/min CLRW with a choice of make-up rates to match your analyzer requirements. The single system design incorporates all the water purification components plus a 50 liter storage reservoir to ensure a quick and cost-effective installation.

Product Specifications MEDICA Pro-30 MEDICA Pro-60 MEDICA Pro-120Inorganics (resistivity at 25°C) >10 MΩ.cm >10 MΩ.cm >10 MΩ.cmOrganics (TOC) <30 ppb <30 ppb <30 ppbBacteria <1 CFU/ml <1 CFU/ml <1 CFU/ml Particles 0.05 μm filter 0.05 μm filter 0.05 μm filterSilica <0.05 mg/l <0.05 mg/l <0.05 mg/lDelivery flow rate Up to 4 l/min @

14psi (1bar)Up to 4 l/min @ 14psi (1bar)

Up to 4 l/min @ 14psi (1bar)

Sterilized by irradiation

Compact for efficient storage

Easy peel-off film Time saving, sterile, pre-filled diluent

Long shelf life Buffers for every industry

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TOC


Questions about TOC?


[email protected]

Product Specifications MEDICA Pro-30 MEDICA Pro-60 MEDICA Pro-120Inorganics (resistivity at 25°C) >10 MΩ.cm >10 MΩ.cm >10 MΩ.cmOrganics (TOC) <30 ppb <30 ppb <30 ppbBacteria <1 CFU/ml <1 CFU/ml <1 CFU/ml Particles 0.05 μm filter 0.05 μm filter 0.05 μm filterSilica <0.05 mg/l <0.05 mg/l <0.05 mg/lDelivery flow rate Up to 4 l/min @

14psi (1bar)Up to 4 l/min @ 14psi (1bar)

Up to 4 l/min @ 14psi (1bar)

Total Organic Carbon - online Total Organic Carbon (TOC) is an index of the total organic matter content of water. A TOC analyzer is widely used for many applications, including raw water management for wastewater treatment; post-treatment effluent and final effluent management; and the monitoring of organic impurities in tap water, cooling water, and washing water. TOC analysis is expected to find a wider range of applications in the future. The TOC-4200 Online Total Organic Carbon Analyzer is an advanced online water quality analyzer that embodies Shimadzu's considerable experience and excellent technical expertise.

TOC analyzersThe TOC-L series analyzers boast a detection limit of 4 μg/L through coordination with NDIR. This is the highest level of detection sensitivity available with the combustion catalytic oxidation method. In addition, the combustion catalytic oxidation method makes it possible to efficiently oxidize not only easily-decomposed, low-molecular-weight organic compounds, but also hard-to-decompose insoluble and macromolecular organic compounds.

Extremely wide measurement range from 4 μg/L to 30,000 mg/L, applicable to everything from ultrapure water to highly contaminated water (TOC-LCSH/CPH)• Capable of TC, IC, TOC (= TC-IC), and NPOC measurement; options enable POC (volatile organic carbon), TOC via POC + NPOC, and even TN (total nitrogen) measurements• The blank check function evaluates system blanks by measuring ultrapure water processed automatically within the instrument• The automatic dilution function enables measurements up to 30,000 mg/L

Reliable sample injection system• Automatic sample acidification and sparging• The automatic dilution function reduces sample salinity, acidity, and alkalinity, significantly extending the period of use of catalysts and combustion tubes (The period of use depends on the sample and measurement conditions.)• Stat or priority samples can be added at anytime to the analysis schedule without interrupting operation even when an autosampler is used

Prices from

179 000,-

Phagocytosis Assay Kit IgG-DyLight™ 405 #601480IgG-DyLight™ 633 #601490

Features• Detect phagocytosis of DyLight™ 405 or 633-labeled, rabbit IgG-opsonized latex beads• Validated in human peripheral blood monocytes and human and mouse monocyte/macrophage cell lines• Phagocytosis detectable in 1-4 hours at 37°C• Assess uptake by flow cytometry or fluorescence microscopy

The Phagocytosis Assay Kit employs latex beads coated with fluorescently-labeled rabbit IgG as a probe for the measurement of the phagocytic process in vitro. The engulfed fluor-escent beads can be detected using a fluor-escence microscope, allowing kinetic studies of phagocytosis at the single-cell level. The flow cytometric readout provides the advantage of visualizing perturbations in phagocytosis on the population level and, when combined with antibody staining, of specific cell types within complex populations. This kit provides enough Latex Beads-Rabbit IgG-DyLight™ 633 or 405 Complex for up to 500 samples.

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FISH • CELL BASED ASSAYS

Promo code NL-1/18 must be referenced when ordering. For terms see the back cover. Promo code NL-1/18 must be referenced when ordering. For terms see the back cover.

Haematology FISH probes for multiple myeloma

Multiple myeloma (MM) is a plasma cell neoplasm, characterised by the accumulation of clonal plasma cells in the bone marrow and by very complex cytogenetic and molecular genetic aberrations. The modal chromosome number in newly diagnosed symptomatic patients is usually either hyperdiploid, with multiple trisomies of chromosomes 3, 5, 7, 9, 11, 15, 19 and 21, or hypodiploid with immunoglobulin heavy chain (Ig) translocations. At disease progression, several genetic progression factors have been identified as deletions of 13q, deletions of 17p and deletion of 1p and/or amplification of 1q. Cytogenetic abnormalities are detected by conventional cytogenetics in about one third of the cases, but FISH increases the proportion of chromosomal abnormality detection to >90%.

FISH probes for Multiple Myeloma is also available as MULTIPROBE

PANEL: Eight FISH probes are hybridized on the same slide in a

spatially separated manner allowing rapid screening of a patient sample for a

number of DNA sequences in a single FISH analysis!

Contact us for more information.

Haematology Probes for

Multiple M

yeloma

Probe Name Chromosome Region Probe Type Control Probe No. Tests Cat. No.13q14.3 13q14.2-q14.3 Deletion D13S1825 5 or 10 LPH 006CKS1B/CDKN2C (P18) 1q21.3/1p32.3 Amplification/Deletion - 5 or 10 LPH 039D13S319 Plus 13q14.2-14.3 Deletion LAMP1 5 or 10 LPH 068D13S25 13q14.3 Deletion D13S1825 5 or 10 LPH 043IGH 14q32.33 Breakapart - 5 or 10 LPH 014IGH Plus 14q32.33 Breakapart - 5 or 10 LPH 070IGH/CCND3 Plus Dual Fusion 14q32.33/6p21 Translocation - 5 or 10 LPH 075IGH/FGFR3 Plus, Dual Fusion 14q32.33/4p16.3 Translocation - 5 or 10 LPH 074IGH/MAF Plus, Dual Fusion 14q32.33/16q23.1-q23.2 Translocation - 5 or 10 LPH 073IGH/MAFB Plus, Dual Fusion 14q32.33/20q12.2 Translocation - 5 or 10 LPH 077IGH/MYEOV Plus, Dual Fusion 14q32.33/11q13.3 Translocation - 5 or 10 LPH 078P53 (TP53) 17P13.1 Deletion D17Z1 5 or 10 LPH 017

NEW! Cell-based assays

ROS Detection Cell-Based Assay Kit (DCFDA) #601520

Features• Measures general ROS directly in living cells• Includes pyocyanin positive control and N-acetyl cysteine negative control• Flow cytometry and plate-based methods

2,7-Dichlorofluoroscin Diacetate (DCFDA or 2,7-dichlorodihydro-fluoroscein diacetate) is a widely used, cell-permeable redox sensitive fluorescent probe that is oxidized by ROS and certain RNS to yield the highly fluorescent product 2,7-dichlorofluoroscein. The ROS Detec-tion Cell-Based Assay Kit (DCFDA) uses DCFDA as a fluorescent probe for the detection of ROS generation directly in living cells. Pyocyanin, a cell-permeable compound capable of redox cycling, is included as a positive control for ROS generation. N-acetyl-cysteine is included as an antioxidant control.

IRF3 (human recombinant) #22811

Interferon regulatory factor 3 (IRF3) plays a crucial role in activation ofinnate immunity and inflammationin response to viral infection, functioning as a molecular switch forantiviral activity. dsRNA generated during a viral infection leads to IRF3 activation by serine/threonine phos-phorylation by TBK1 or IKBKE kinases, which induces a confor-mational change leading to its dimerization, nuclear localization, and association with CREBBP. The complex, known as DRAF1, activates transcription interferons alpha and beta, as well as other interferon-induced genes. These genes play a critical role in type 1 interferon (IFN)-dependent immune response. A recent study shows phospho-rylation of innate immune adaptor proteins MAVS, STING, and TRIF recruits and specifies IRF3 phospho-rylation and activation by the serine/threonine-protein kinase TBK1, thereby inducing the pro-duction of type-1 interferons.

Introduction offer

-20%on all three

kits

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LABORATORY EQUIPMENT


We recommend performing all liquid handling operations in DNA/RNA UV-Cleaner Cabinet to protect samples from contamination.

Additional processing of the sample is often needed before NA extraction. Select appropriate instruments for sample preparation, depending on the sample type. For mixing and resuspen-sion choose vortex V-1 Plus or shaker MPS-1, depending on the amount of samples. For sedimentation choose centrifuge Microspin 12. Multi-motion programmable rotator Multi Bio RS-24 is ideal for mixing.

Cell lysis is a destruction of cell mem-branes with the lysis buffer. Depending on the protocol it is performed at the room temperature or higher. To ensure the most comprehensive lysis of your samples choose our Dry Blocks or Thermo-Shakers that provide one of the highest temperature uniformity and accuracy on the market.

Lysis procedure is followed by binding of the nucleic acid to magnetic particles. The magnetic rack is used to immobilize magnetic beads with the bound nucleic acid and then, protein and contaminants are removed by washing with wash buffers.Biosan Magnetic rack contains a high-performance magnet allowing instantaneous and most complete bead collection from the solution and providing the most extensive NA recovery. To facilitate numerous aspiration steps we recommend using vacuum aspirator.

The nucleic acid is then eluted from the magnetic beads with the elution buffer. Often, elution step requires incubation to release nucleic acids from the magnetic beads. Choose a thermostat from our Dry Blocks and Thermo-Shakers.

Mixing and resuspension

V-1 plus, Personal Vortex

MPS-1, High–Speed Multi Plate Shaker

Multi Bio RS-24, Programmable rotator

Centrifugation Microspin 12, High-speed Mini-centrifuge

Incubation TS-100C, Thermo-Shaker

TDB-120, Dry block thermostat

Magnetic bead separation

Aspiration FTA-2i, Aspirator with trap flask

Assist Series, Pipettes

Incubation TDB-100, Dry block thermostat

TS-100, Thermo-Shaker

Sample preparation and NA extraction

UVC-T-M-AR, more DNA/RNA UV-Cleaner Cabinets at www.biosan.lv

Manual Nucleic Acid Extraction Utilizing Magnetic Bead Technology

SAM

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UTI

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MagSorb 16, Magnetic rack

Biosan SIA., Ratsupites 7, build. 2, Riga, LV-1067, Latvia, Phone: +371 674 261 37, Fax: +371 674 281 01, E-mail: [email protected], http://www.biosan.lv Medical–Biological

Research & Technologies

Biosan presents the complete line of necessary instrumentation to utilize magnetic bead extraction kits and protocols from various manu-facturers and meet the most demanding user requirements.

Different manufacturers offer wide range of magnetic NA extraction kits, but all of them are based on magnetic particles and utilize the same principles of extraction. Every step of extraction on magnetic particles is crucial, so it is important to choose the right equipment for effective NA purification.

Nucleic acid isolation and purification on magnetic beads usually consists of four basic steps: sample preparation, cell lysis, washing and elution.

BioNordika AS • tel 23 03 58 00 • [email protected] • www.bionordika.no

Promo code: NL-1/18Note! The promo code is required to obtain the special prices!

The offers are valid until 31.03.2018 and cannot be combined with other offers or agreements. The prices are listed excl. VAT and shipping charges.

We reserve the right for typos.

NEXIS GC - 2030 The Next Industry Standard

Shimadzu`s premier gas chromatograph, offers a modern approach to a classic chromatograph technique. Designed with the user in mind, new innovative features, exceptional performance and high throughput capabilities will elevate your lab to the next level.

Redefining the chromatography experience • Smart Interface• Intuitive colorful LCD display• Simple and clear touch controls• Tool free and easy maintenance• Ovenlight & ClickTek columns• World class precision • Main settings performable via a smart device

World’s highest sensitivity and reproducibility • High-sensitivity detectors support a wide variety of analyses• Includes high sensitivity detectors like the new FID and BID• Intelligent flow controller with exceptional reproducibility

Unmatched throughput • Specialized analyzers built specifically for your needs• Advanced Flow Technology (AFT) expansion• Optional hydrogen sensor for secure customers working with hydrogen gas

Questions about gas chromatography?


[email protected]

Documents

Introducing GenScript as our new supplierbionordika.no/application/files/1015/1488/3805/... · Reactor-Ready lab reactor features • Rapid, tool-free vessel exchange with quick-release