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2 |Biochemistry laboratory
1. Aims of Biochemistry Experiments
Experiments Rules
Instruments Introduction
GRADING
Theory EXAM : 70%Attendance(Theory): 10%Lab Performance: 20%
4 |
2. Experiment Rules
① Put on your white lab coat before the class begin
Biochemistry laboratory
5 |
2. Experiment Rules
② It is better done than said
Biochemistry laboratory
6 |
2. Experiment Rules
③ Make Compensation
For Any Damage
Biochemistry laboratory
7 |
2. Experiment Rules
④ Please shut off
the faucet and Power
Biochemistry laboratory
8 |
2. Experiment Rules
⑤ Clean the classroom before you leave!
Biochemistry laboratory
If you break any rules above, I will lower your
participation grade !
10 |
3. Instruments Introduction
① check instruments for each group by the table below
category number category number
250-ml beaker 1 Dropper 1
100-ml beaker 1 Glass stirring rod 1
Large Test tube 18 Test-tube rack 1
Small Test tube 6 Bulb for pipette 1
Biochemistry laboratory
11 |
3. Instruments Introduction
Beaker
Biochemistry laboratory
12 |
3. Instruments Introduction
Test tube
Biochemistry laboratory
Solution Mixing
Rotation
Fliping
Shaking
Biochemistry laboratory
3. Instruments Introduction
Test-tube
rack
Biochemistry laboratory
15 |
3. Instruments Introduction
Test-tube
brush
Biochemistry laboratory
Test tube Washing
1. Flush the test tube with water At least 3 times
2. Scrub it with detergent both inside & outside
3. Flush the test tube with water at least 5 times just like step1
4. Rinse it with distilled water and place
them upside down on the rack to leave it dry
Biochemistry laboratory
17 |
3. Instruments Introduction
Dropper
Biochemistry laboratory
3. Instruments Introduction
Glass-stirring rod
Biochemistry laboratory
19 |
3. Instruments Introduction
Tube clip
Biochemistry laboratory
3. Instruments Introduction
Bulb for pipette
Biochemistry laboratory
3. Instruments Introduction
Scaled pipette
Biochemistry laboratory
Scaled Pipette Pattern:
0.5 ml 、 1 ml 、 2 ml
5 ml 、 10 ml etc.
Biochemistry laboratory
• Hold Like This
Biochemistry laboratory
Usage
Hold pipette
Absorb Solution
Adjust the Volume
Drop Solution
Biochemistry laboratory
3. Instruments Introduction
Micropipette
Biochemistry laboratory
Micropipette Specifications: 10 μl 、 20 μl 、 100 μl 、 200 μl 、 1000 μl & 5000 μl
Biochemistry laboratory
THE INTRODUCTION ON PIPETTE
Biochemistry laboratory
THE INTRODUCTION ON PIPETTE
Biochemistry laboratory
Usage
1. Set the volume of solution
2. Choose paired tip ,and put on it
3. Absorb solution with 1st Lock , drop solution with 2nd Lock.
Summary
1. Be careful
2. Be patient
3. Be lively
Biochemistry laboratory
Please hand in your Report in time !
Thank youBiochemistry laboratory
Changsha Medical University
LAB REPORT
LAB REPORT
LAB REPORT