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Introduction to the Analysis of Variance (ANOVA) Computing One-Way Independent Measures (Between Subjects) ANOVAs

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Page 1: Introduction to the Analysis of Variance - Rutgers Universitymmm431/quant_methods_F14/QM... · 2014-10-30 · Introduction to the Analysis of Variance (ANOVA) ... 0 1 D 5 10 0 1 1

Introduction to the Analysis of Variance

(ANOVA)

Computing One-Way Independent Measures

(Between Subjects) ANOVAs

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Analysis of Variance (ANOVA)

• The analysis of variance (ANOVA) is a statistical technique for testing for differences between the means of multiple (more than two) groups

• It is probably the most prevalent statistical technique used in psychological research.

• The ANOVA is a flexible technique that can be used with a variety of different research designs.

• In today’s lecture, I will explain the logic behind the ANOVA and introduce the one-way between groups ANOVA, which is an ANOVA in which the groups are defined along only one independent (or quasi-independent) variable

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Intro to ANOVA

The Analysis of Variance

• The purpose of ANOVA is much the same as the t tests

presented in the preceding lectures

– Are the mean differences obtained for sample data sufficiently large for

us to conclude that there are mean differences between the populations

from which the samples were obtained

• The difference between ANOVA and the t tests is that ANOVA

can be used in situations where there are two or more means

being compared, whereas the t tests are limited to situations

where only two means are involved.

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Intro to ANOVA

The Problem of Multiple Comparisons

• The ANOVA is necessary to protect researchers from an

excessive experimentwise error rate in situations where a

study is comparing more than two population means.

– Experimentwise error rate: the probability of making at least one Type I

error across mutliple comparisons

• These situations would require a series of several t tests to

evaluate all of the mean differences. (Remember, a t test can

compare only two means at a time)

• So? Why not just use multiple t-tests?

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Problem of Multiple Comparisons

• Why not just use multiple t-tests?

– Although each t test can be evaluated using a specific α-level (risk of

Type I error), the α-levels accumulate over a series of tests so that the

final familywise α-level can be quite large

• Example:

– For 5 levels of the independent variable, there are 10 possible pairwise

comparisons between group means:

• {1,2},{1,3},{1,4},{1,5},{2,3},{2,4},{2,5},{3,4},{3,5},{4,5}

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Problem of Multiple Comparisons

• Assume H0 is true and α=0.05. Then the probability of accepting H0 in a single pairwise comparison is:

• However, we have to make 10 such comparisons. Using the multiplicative law of probability (remember that?), and assuming independent pairwise tests, the probability of correctly retaining the null in all 10 comparisons is:

0accept single pairwise 0.91 5P H

0

10

1 1 ... 1

0.59

accept all

9

0.95

P H

01 accept all

1 0.599 0.401

experiment P H

We now have a 40% overall

chance of making a Type I error!

Therefore,

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01:830:200:01-05 Fall 2014

Intro to ANOVA

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Intro to ANOVA

Null and Alternative Hypotheses in ANOVAs

• The omnibus null hypothesis is the null hypothesis in the

ANOVA: that the population means of all groups being

compared are equal

– i.e., for three groups, H0: μ1= μ2= μ3

• Alternative Hypothesis: at least one population mean is

different from the others.

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Intro to ANOVA

Assumptions of the ANOVA

• Normality of Scores – I.e., we assume that the scores in all of our group populations are

normally distributed

– Since this is important primarily for the sampling distribution of the mean, the ANOVA is fairly robust to violations of this assumption, especially if the sample sizes are reasonably large

• Homogeneity of variances – We assume that each population of scores has the same variance

– E.g., [error variance]

– ANOVA is fairly robust to violations of this assumption

• Independence of observations – E.g., given the population parameters, knowing one person’s score tells

you nothing about another person’s score.

– Violations of this assumption can have serious implications for an analysis.

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Populations

(µ,σ unknown)

Samples

Instructor 1 Instructor 2 Instructor 3

Alternative Hypothesis: µ1 , µ2 , and µ3 are not all equal

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Omnibus Null Hypothesis:

µ1 = µ2 = µ3

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the Analysis of Variance

• The test statistic for ANOVA is an F-ratio, which is a ratio of two estimates of the population variance.

• In the context of ANOVA, these variance estimates are called mean squares, or MS values

– The numerator, MSbetween, estimates variance using the sample means

of different treatment groups

– The denominator, MSwithin (or MSerror), estimates variance using the sample variances within each treatment group

variance including any treatment effects

variance without any treatment effects

between

within

MSF

MS

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the Analysis of Variance

Total Variance

Between

Treatments

Variance

Within

Treatments

Variance

Measures differences caused by:

• Systematic treatment effects

• Sampling & other non-

systematic errors

Measures differences caused by:

• Sampling & other non-

systematic errors

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

• Regardless of whether or not the null hypothesis is true, the

assumption of homogeneity of variances implies that all

population variances are equal

• Thus, as we did for the independent-samples t-test, we can

estimate this shared population variance by taking the

average of the sample variances (the pooled variance)

1

2 2

2

2 2

3

2 32

2 2 22 2 2 2 2

31

1 , ,3

ˆwithi pn

s s ss Avg s s s

(assuming n1 = n2 = n3)

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

• However, if all the population means are equal (under H0),

then we have a second way to estimate the population

variance

– we can estimate the population variance using the variance of the

sample means

• Recall that the Central Limit Theorem tells us how to compute

the variance of sample means from the population variance:

• We can rearrange this formula to solve for the population

variance given the variance of sample means:

22

Mn

2 2

Mn

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

• Of course, we don’t have the variance of sample means

either. However, we can estimate it by computing the variance

of our three group means

• Plugging this into the previous equation, our second estimate

of the population variance is

1 2 3

2 2 , ,ˆM Ms Var M M M

2 2ˆbetween Mns

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

We now have two estimates of the population variance:

• An estimate computed from the sample variances, which

should estimate the population variance regardless of whether

H0 is true

• A second estimate computed from the sample means, which

only estimates the population variance if H0 is true

2 3

2 2 2 2 2

1 , ,ˆwith n pi s Avg s s s

2 2

2 31ˆ , ,betwe Men ns nVar M M M

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

• The F-ratio used as the test statistic for the ANOVA is simply the

ratio between these two estimates of the population variance

• If H0 is true, then these two estimates should be equal (on average)

– In this case, the ratio should be 1.0

• However, if H0 is false, then the estimate in the numerator (which is

based on the variability of sample means) will include the treatment

effect in addition to differences in sample means expected by

chance

– In this case, the ratio should be greater than 1.0

21

2

2 3

2 3

2 2 2

1

, ,

,

ˆ

ˆ ,

between

with

between

i inwith n

nVar M MMSF

MS A

M

vg s s s

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Intro to ANOVA

The F distribution

reject H0

retain H0

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Populations

Samples

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01:830:200:01-05 Fall 2014

Intro to ANOVA

The Logic of the ANOVA

2 2

2 2 2

1 2 3

3

12.1

ˆ

36.

8 33.18 63.52

3

29

within ps

s s s

𝑴 𝑴𝟐

65.40 4277.16

70.95 5033.90

71.20 5069.44

sum 207.55 14380.50

207.55

369.18TM

M

k

n M

n

2

22 207.55

14380.503

14380.50 14359.00 2= 1.50

M

MMSS

k

2 2

215.0

21.5020

2

ˆbetween

M

M

M

ns

SSndf

2

2

2155.92

36 9ˆ .2

ˆbetween

within

F

Sample 1 Sample 2 Sample 3

n = 20 n = 20 n = 20

M = 65.4 M = 70.95 M = 71.2

s2 = 12.18 s2 = 33.18 s2 = 63.52

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computations for the ANOVA

• In computing the terms required for the F-statistic, we won’t

explicitly compute any sample variances or standard

deviations

• Instead, in all intermediate steps, we’ll deal exclusively with

sums of squared deviations (SS) and means of squared

deviations (MS)

• Computing the F-statistic using sample standard deviations or

variances gets you the same answer, but requires more

calculations

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Intro to ANOVA

Computing the F-statistic

betweenbetween

between

SSMS

df within

within

within

SSMS

df

, betweenbetween within

within

MSF df df

MS

where,

and

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computations for the ANOVA: Preliminaries

• Start by computing and for each group, then

compute:

• Grand total: The overall total, computed over all scores in all

groups (samples)

• Total sum of squared scores: The sum of squared scores

computed over all scores in all groups

k n

T ij

i j

x x

2 2k n

T ij

i j

x x

x 2x

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computations for the ANOVA: SS terms

• SStotal : The sum of squared deviations of all observations

from the grand mean

– Not strictly needed for computing the F ratio, but it makes computing the

needed SS terms much easier

2

22

total T

T

T

xxSS x M

N

or

total between withinSSSS SS

(conceptual) (computational)

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Total Variance

Between

Treatments

Variance

Within

Treatments

Variance

Computations for the ANOVA: SS terms

total between within

total between within

SS SS

d

SS

d dfff

between total within

between total within

SS SS

d

SS

d dfff

within total between

within total between

SS SS

d

SS

dfdff

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computations for the ANOVA: SS terms

• SSbetween: The sum of squared deviations of the sample

means from the grand mean multiplied by the number of

observations

• SSwithin (SSerror): The sum of squared deviations within each

sample

2

k

between i i T

i

nS M MS

within total betweenSS SSSS 1 2 ...k

within j

j

kSS SS SS SS SS or

or totb aet lween withinSS SSSS

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computations for the ANOVA: df terms

• dftotal = N-1 :

– degrees of freedom associated with SStotal

– N is the total number of scores

• dfbetween = k-1 :

– degrees of freedom associated with SSbetween

– k is the number of groups (samples)

• dfwithin (or dferror)= dftotal -dfbetween = N-k :

– degrees of freedom associated with SSwithin

– Can also be computed as:

1 2 1 2. 1 .. 1.. 1 .k kdf d nfdf n n

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Computing the F-statistic

betweenbetween

between

SSMS

df

withinwithin

within

SSMS

df

, betweenbetween within

within

MSF df df

MS

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Intro to ANOVA

The One-Way ANOVA: Steps

1. State Hypotheses

2. Compute F-ratio statistic:

– For data in which I give you raw scores, you will have to compute:

• Sample means

• SStotal, SSbetween, & SSwithin

• dftotal, dfbetween, & dfwithin

3. Use F-ratio distribution table to find critical F-value representing rejection region

4. Make a decision: does the F-statistic for your sample fall into the rejection region?

, betweenbetween within

within

MSF df df

MS

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Intro to ANOVA

A psychologist wants to determine whether having a job

interferes with student academic performance. She measures

academic performance using students’ GPAs. She selects a

sample of 30 students.

• Of these students,10 did not work, 10 worked part-time, and

10 worked full-time during the previous semester

• Conduct an ANOVA at a .05 level of significance testing the

hypothesis that having a job interferes with student

performance

The One-Way ANOVA: Textbook Example

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Intro to ANOVA

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

Source df SS MS F

Between

Within (error)

Total

Set up a summary ANOVA table:

1. Compute degrees of freedom

1

29

2

27

1total

between

within

df k

df

df N

N k

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Intro to ANOVA

Source df SS MS F

Between 2

Within (error) 27

Total 29

Set up a summary ANOVA table:

2. Compute SStotal

2

2

297.10

319.4730

319.47 5314.2 . 98 1

total

T

T

xSS x

N

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

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Intro to ANOVA

Source df SS MS F

Between 2

Within (error) 27

Total 29 5.19

Set up a summary ANOVA table:

3. Compute SSbetween (or SSwithin) directly

2

2 2 210 3.43 3.24 10 3.3 3.24 10 2.98 3.24

0.361 0.036 0.6 076 1. 7

between TSS n M M

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

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Intro to ANOVA

Source df SS MS F

Between 2 1.07

Within (error) 27

Total 29 5.19

Set up a summary ANOVA table:

4. Compute the missing SS value

(SSbetween or SSwithin) via subtraction:

5.19 11.07 4. 2

total betweenwithinSS SS SS

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

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Intro to ANOVA

Source df SS MS F

Between 2 1.07

Within (error) 27 4.12

Total 29 5.19

Set up a summary ANOVA table:

5. Compute the MS values needed to

compute the F ratio:

0.531.07

25between

between

between

SSMS

df

4.12

23

70.15within

within

within

SSMS

df

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

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Intro to ANOVA

Source df SS MS F

Between 2 1.07 0.535

Within (error) 27 4.12 0.153

Total 29 5.19

Set up a summary ANOVA table:

6. Compute the F ratio:

3.500.535

2,270

,

.153

betwerro

eenb r

er

et

ror

ween

MSF df

Mf

F

dS

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

Tx 97.10

2

Tx 319.47

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Intro to ANOVA

1 2 3 4 5 6 7 8 9 10

1 161.45 199.50 215.71 224.58 230.16 233.99 236.77 238.88 240.54 241.88

2 18.51 19.00 19.16 19.25 19.30 19.33 19.35 19.37 19.38 19.40

3 10.13 9.55 9.28 9.12 9.01 8.94 8.89 8.85 8.81 8.79

4 7.71 6.94 6.59 6.39 6.26 6.16 6.09 6.04 6.00 5.96

5 6.61 5.79 5.41 5.19 5.05 4.95 4.88 4.82 4.77 4.74

6 5.99 5.14 4.76 4.53 4.39 4.28 4.21 4.15 4.10 4.06

7 5.59 4.74 4.35 4.12 3.97 3.87 3.79 3.73 3.68 3.64

8 5.32 4.46 4.07 3.84 3.69 3.58 3.50 3.44 3.39 3.35

9 5.12 4.26 3.86 3.63 3.48 3.37 3.29 3.23 3.18 3.14

10 4.96 4.10 3.71 3.48 3.33 3.22 3.14 3.07 3.02 2.98

11 4.84 3.98 3.59 3.36 3.20 3.09 3.01 2.95 2.90 2.85

12 4.75 3.89 3.49 3.26 3.11 3.00 2.91 2.85 2.80 2.75

13 4.67 3.81 3.41 3.18 3.03 2.92 2.83 2.77 2.71 2.67

14 4.60 3.74 3.34 3.11 2.96 2.85 2.76 2.70 2.65 2.60

15 4.54 3.68 3.29 3.06 2.90 2.79 2.71 2.64 2.59 2.54

16 4.49 3.63 3.24 3.01 2.85 2.74 2.66 2.59 2.54 2.49

17 4.45 3.59 3.20 2.96 2.81 2.70 2.61 2.55 2.49 2.45

18 4.41 3.55 3.16 2.93 2.77 2.66 2.58 2.51 2.46 2.41

19 4.38 3.52 3.13 2.90 2.74 2.63 2.54 2.48 2.42 2.38

20 4.35 3.49 3.10 2.87 2.71 2.60 2.51 2.45 2.39 2.35

22 4.30 3.44 3.05 2.82 2.66 2.55 2.46 2.40 2.34 2.30

24 4.26 3.40 3.01 2.78 2.62 2.51 2.42 2.36 2.30 2.25

26 4.23 3.37 2.98 2.74 2.59 2.47 2.39 2.32 2.27 2.22

28 4.20 3.34 2.95 2.71 2.56 2.45 2.36 2.29 2.24 2.19

30 4.17 3.32 2.92 2.69 2.53 2.42 2.33 2.27 2.21 2.16

40 4.08 3.23 2.84 2.61 2.45 2.34 2.25 2.18 2.12 2.08

50 4.03 3.18 2.79 2.56 2.40 2.29 2.20 2.13 2.07 2.03

60 4.00 3.15 2.76 2.53 2.37 2.25 2.17 2.10 2.04 1.99

120 3.92 3.07 2.68 2.45 2.29 2.18 2.09 2.02 1.96 1.91

200 3.89 3.04 2.65 2.42 2.26 2.14 2.06 1.98 1.93 1.88

500 3.86 3.01 2.62 2.39 2.23 2.12 2.03 1.96 1.90 1.85

1000 3.85 3.00 2.61 2.38 2.22 2.11 2.02 1.95 1.89 1.84

dfnumerator

F table for α=0.05

reject H0

df e

rro

r

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Intro to ANOVA

1 2 3 4 5 6 7 8 9 10

1 161.45 199.50 215.71 224.58 230.16 233.99 236.77 238.88 240.54 241.88

2 18.51 19.00 19.16 19.25 19.30 19.33 19.35 19.37 19.38 19.40

3 10.13 9.55 9.28 9.12 9.01 8.94 8.89 8.85 8.81 8.79

4 7.71 6.94 6.59 6.39 6.26 6.16 6.09 6.04 6.00 5.96

5 6.61 5.79 5.41 5.19 5.05 4.95 4.88 4.82 4.77 4.74

6 5.99 5.14 4.76 4.53 4.39 4.28 4.21 4.15 4.10 4.06

7 5.59 4.74 4.35 4.12 3.97 3.87 3.79 3.73 3.68 3.64

8 5.32 4.46 4.07 3.84 3.69 3.58 3.50 3.44 3.39 3.35

9 5.12 4.26 3.86 3.63 3.48 3.37 3.29 3.23 3.18 3.14

10 4.96 4.10 3.71 3.48 3.33 3.22 3.14 3.07 3.02 2.98

11 4.84 3.98 3.59 3.36 3.20 3.09 3.01 2.95 2.90 2.85

12 4.75 3.89 3.49 3.26 3.11 3.00 2.91 2.85 2.80 2.75

13 4.67 3.81 3.41 3.18 3.03 2.92 2.83 2.77 2.71 2.67

14 4.60 3.74 3.34 3.11 2.96 2.85 2.76 2.70 2.65 2.60

15 4.54 3.68 3.29 3.06 2.90 2.79 2.71 2.64 2.59 2.54

16 4.49 3.63 3.24 3.01 2.85 2.74 2.66 2.59 2.54 2.49

17 4.45 3.59 3.20 2.96 2.81 2.70 2.61 2.55 2.49 2.45

18 4.41 3.55 3.16 2.93 2.77 2.66 2.58 2.51 2.46 2.41

19 4.38 3.52 3.13 2.90 2.74 2.63 2.54 2.48 2.42 2.38

20 4.35 3.49 3.10 2.87 2.71 2.60 2.51 2.45 2.39 2.35

22 4.30 3.44 3.05 2.82 2.66 2.55 2.46 2.40 2.34 2.30

24 4.26 3.40 3.01 2.78 2.62 2.51 2.42 2.36 2.30 2.25

26 4.23 3.37 2.98 2.74 2.59 2.47 2.39 2.32 2.27 2.22

28 4.20 3.34 2.95 2.71 2.56 2.45 2.36 2.29 2.24 2.19

30 4.17 3.32 2.92 2.69 2.53 2.42 2.33 2.27 2.21 2.16

40 4.08 3.23 2.84 2.61 2.45 2.34 2.25 2.18 2.12 2.08

50 4.03 3.18 2.79 2.56 2.40 2.29 2.20 2.13 2.07 2.03

60 4.00 3.15 2.76 2.53 2.37 2.25 2.17 2.10 2.04 1.99

120 3.92 3.07 2.68 2.45 2.29 2.18 2.09 2.02 1.96 1.91

200 3.89 3.04 2.65 2.42 2.26 2.14 2.06 1.98 1.93 1.88

500 3.86 3.01 2.62 2.39 2.23 2.12 2.03 1.96 1.90 1.85

1000 3.85 3.00 2.61 2.38 2.22 2.11 2.02 1.95 1.89 1.84

dfnumerator

F table for α=0.05

reject H0

df e

rro

r

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Intro to ANOVA

Source df SS MS F

Between 2 1.07 0.535 3.50

Within (error) 27 4.12 0.153

Total 29 5.19

Set up a summary ANOVA table:

7. Compare computed F statistic with

Fcrit and make a decision

03.5 3.37;

3.3

reject

7critF

H

Conclusion: Having a job does significantly interfere with academic performance

Work Status No Work Part-Time Full-Time 3.40 3.50 2.90

3.20 3.60 3.00

3.00 2.70 2.60

3.00 3.50 3.30

3.50 3.80 3.70

3.80 2.90 2.70

3.60 3.40 2.40

4.00 3.20 2.50

3.90 3.30 3.30

2.90 3.10 3.40

M1 =3.43 M2 =3.3 M3 =2.98 MT =3.24

n1 =10 n2 =10 n3 =10 N =30

TSS = 5.19

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Intro to ANOVA

The One-way ANOVA: Example 2

Return to our running example:

Do test scores vary as a function of the instructor?

• x1 : sample scores from Dr. M’s class

• x2 : sample scores from Dr. K’s class

• x3 : sample scores from Dr. A’s class

• Null Hypothesis H0: µ1 = µ2 = µ3

• Research Hypothesis H1: one of the population means is different

• Do we accept or reject the null hypothesis? – Assume α = 0.05

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Intro to ANOVA

Source df SS MS F

Between

Within (error)

Total 465.04

Set up a summary ANOVA table:

1. Compute degrees of freedom

1

24

2

22

1total

between

within

df k

df

df N

N k

Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

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Intro to ANOVA

Source df SS MS F

Between 2

Within (error) 22

Total 24 465.04

Set up a summary ANOVA table:

89.71 91.60 68.0

249.31

0

withinSS SS

Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

3. Compute SSwithin (or SSbetween) directly

(This time, we’ll compute SSwithin)

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Intro to ANOVA

Set up a summary ANOVA table:

465.04 249.

21 3

31

5.7

total withbetween inSSS SSS

Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

Source df SS MS F

Between 2

Within (error) 22 249.31

Total 24 465.04

4. Compute the missing SS value

(SSbetween or SSwithin) via subtraction:

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Intro to ANOVA

Set up a summary ANOVA table: Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

Source df SS MS F

Between 2 215.73

Within (error) 22 249.31

Total 24 465.04

5. Compute the MS values needed to

compute the F ratio:

215.73

2107.87between

between

between

SSMS

df

249.31

211.33

2

withinwithin

within

SSMS

df

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Intro to ANOVA

Set up a summary ANOVA table: Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

Source df SS MS F

Between 2 215.73 107.87

Within (error) 22 249.31 11.33

Total 24 465.04

6. Compute the F ratio:

9.52107.87

2,221

,

1.33

betwerro

eenbet r

error

ween

MSF df

Mf

F

dS

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Intro to ANOVA

1 2 3 4 5 6 7 8 9 10

1 161.45 199.50 215.71 224.58 230.16 233.99 236.77 238.88 240.54 241.88

2 18.51 19.00 19.16 19.25 19.30 19.33 19.35 19.37 19.38 19.40

3 10.13 9.55 9.28 9.12 9.01 8.94 8.89 8.85 8.81 8.79

4 7.71 6.94 6.59 6.39 6.26 6.16 6.09 6.04 6.00 5.96

5 6.61 5.79 5.41 5.19 5.05 4.95 4.88 4.82 4.77 4.74

6 5.99 5.14 4.76 4.53 4.39 4.28 4.21 4.15 4.10 4.06

7 5.59 4.74 4.35 4.12 3.97 3.87 3.79 3.73 3.68 3.64

8 5.32 4.46 4.07 3.84 3.69 3.58 3.50 3.44 3.39 3.35

9 5.12 4.26 3.86 3.63 3.48 3.37 3.29 3.23 3.18 3.14

10 4.96 4.10 3.71 3.48 3.33 3.22 3.14 3.07 3.02 2.98

11 4.84 3.98 3.59 3.36 3.20 3.09 3.01 2.95 2.90 2.85

12 4.75 3.89 3.49 3.26 3.11 3.00 2.91 2.85 2.80 2.75

13 4.67 3.81 3.41 3.18 3.03 2.92 2.83 2.77 2.71 2.67

14 4.60 3.74 3.34 3.11 2.96 2.85 2.76 2.70 2.65 2.60

15 4.54 3.68 3.29 3.06 2.90 2.79 2.71 2.64 2.59 2.54

16 4.49 3.63 3.24 3.01 2.85 2.74 2.66 2.59 2.54 2.49

17 4.45 3.59 3.20 2.96 2.81 2.70 2.61 2.55 2.49 2.45

18 4.41 3.55 3.16 2.93 2.77 2.66 2.58 2.51 2.46 2.41

19 4.38 3.52 3.13 2.90 2.74 2.63 2.54 2.48 2.42 2.38

20 4.35 3.49 3.10 2.87 2.71 2.60 2.51 2.45 2.39 2.35

22 4.30 3.44 3.05 2.82 2.66 2.55 2.46 2.40 2.34 2.30

24 4.26 3.40 3.01 2.78 2.62 2.51 2.42 2.36 2.30 2.25

26 4.23 3.37 2.98 2.74 2.59 2.47 2.39 2.32 2.27 2.22

28 4.20 3.34 2.95 2.71 2.56 2.45 2.36 2.29 2.24 2.19

30 4.17 3.32 2.92 2.69 2.53 2.42 2.33 2.27 2.21 2.16

40 4.08 3.23 2.84 2.61 2.45 2.34 2.25 2.18 2.12 2.08

50 4.03 3.18 2.79 2.56 2.40 2.29 2.20 2.13 2.07 2.03

60 4.00 3.15 2.76 2.53 2.37 2.25 2.17 2.10 2.04 1.99

120 3.92 3.07 2.68 2.45 2.29 2.18 2.09 2.02 1.96 1.91

200 3.89 3.04 2.65 2.42 2.26 2.14 2.06 1.98 1.93 1.88

500 3.86 3.01 2.62 2.39 2.23 2.12 2.03 1.96 1.90 1.85

1000 3.85 3.00 2.61 2.38 2.22 2.11 2.02 1.95 1.89 1.84

dfnumerator

F table for α=0.05

reject H0

df e

rro

r

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Intro to ANOVA

1 2 3 4 5 6 7 8 9 10

1 161.45 199.50 215.71 224.58 230.16 233.99 236.77 238.88 240.54 241.88

2 18.51 19.00 19.16 19.25 19.30 19.33 19.35 19.37 19.38 19.40

3 10.13 9.55 9.28 9.12 9.01 8.94 8.89 8.85 8.81 8.79

4 7.71 6.94 6.59 6.39 6.26 6.16 6.09 6.04 6.00 5.96

5 6.61 5.79 5.41 5.19 5.05 4.95 4.88 4.82 4.77 4.74

6 5.99 5.14 4.76 4.53 4.39 4.28 4.21 4.15 4.10 4.06

7 5.59 4.74 4.35 4.12 3.97 3.87 3.79 3.73 3.68 3.64

8 5.32 4.46 4.07 3.84 3.69 3.58 3.50 3.44 3.39 3.35

9 5.12 4.26 3.86 3.63 3.48 3.37 3.29 3.23 3.18 3.14

10 4.96 4.10 3.71 3.48 3.33 3.22 3.14 3.07 3.02 2.98

11 4.84 3.98 3.59 3.36 3.20 3.09 3.01 2.95 2.90 2.85

12 4.75 3.89 3.49 3.26 3.11 3.00 2.91 2.85 2.80 2.75

13 4.67 3.81 3.41 3.18 3.03 2.92 2.83 2.77 2.71 2.67

14 4.60 3.74 3.34 3.11 2.96 2.85 2.76 2.70 2.65 2.60

15 4.54 3.68 3.29 3.06 2.90 2.79 2.71 2.64 2.59 2.54

16 4.49 3.63 3.24 3.01 2.85 2.74 2.66 2.59 2.54 2.49

17 4.45 3.59 3.20 2.96 2.81 2.70 2.61 2.55 2.49 2.45

18 4.41 3.55 3.16 2.93 2.77 2.66 2.58 2.51 2.46 2.41

19 4.38 3.52 3.13 2.90 2.74 2.63 2.54 2.48 2.42 2.38

20 4.35 3.49 3.10 2.87 2.71 2.60 2.51 2.45 2.39 2.35

22 4.30 3.44 3.05 2.82 2.66 2.55 2.46 2.40 2.34 2.30

24 4.26 3.40 3.01 2.78 2.62 2.51 2.42 2.36 2.30 2.25

26 4.23 3.37 2.98 2.74 2.59 2.47 2.39 2.32 2.27 2.22

28 4.20 3.34 2.95 2.71 2.56 2.45 2.36 2.29 2.24 2.19

30 4.17 3.32 2.92 2.69 2.53 2.42 2.33 2.27 2.21 2.16

40 4.08 3.23 2.84 2.61 2.45 2.34 2.25 2.18 2.12 2.08

50 4.03 3.18 2.79 2.56 2.40 2.29 2.20 2.13 2.07 2.03

60 4.00 3.15 2.76 2.53 2.37 2.25 2.17 2.10 2.04 1.99

120 3.92 3.07 2.68 2.45 2.29 2.18 2.09 2.02 1.96 1.91

200 3.89 3.04 2.65 2.42 2.26 2.14 2.06 1.98 1.93 1.88

500 3.86 3.01 2.62 2.39 2.23 2.12 2.03 1.96 1.90 1.85

1000 3.85 3.00 2.61 2.38 2.22 2.11 2.02 1.95 1.89 1.84

dfnumerator

F table for α=0.05

reject H0

df e

rro

r

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Intro to ANOVA

Set up a summary ANOVA table: Instructor

Dr. M Dr. K Dr. A

73 62 72

71 66 68

76 66 70

68 66 62

65 58 69

72 61 66

75 67 65

67 68

67

62

n1 =7 n2 =10 n3 =8 N =25

M1 =71.43 M2 =64.20 M3 =67.50 MT =67.28

SS1 =89.71 SS2 =91.60 SS3 =68.00 SST =465.04

Source df SS MS F

Between 2 215.73 107.87 9.52

Within (error) 22 249.31 11.33

Total 24 465.04

7. Compare computed F statistic with

Fcrit and make a decision

09.52 3.4

3.

4;

4

reject

4critF

H

Conclusion: Student test scores do vary across instructors

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Intro to ANOVA

Effect Size for the One-Way ANOVA

• For ANOVAs, effect sizes are usually indicated using the

R2-family measure eta-squared (η2)

• R2-family measures indicate the effect size in terms of

proportion of variance accounted for by the treatment effect(s)

For our example:

2 0215.73

465.04.46between

total

SS

SS

2 variability explained by treatment effect

total variabilityR

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Intro to ANOVA

Post-hoc Tests for Multiple Comparisons

• Rejecting H0 only tells us that the omnibus null hypothesis

(that all sample means are equal) is false

• However, we are often interested in knowing which particular

means differ from each other

• Evaluating differences (usually pairwise) beyond the omnibus

null hypothesis requires post-hoc testing

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Intro to ANOVA

Post-hoc Tests

• The challenge in constructing a post-hoc multiple comparison test is keeping the experimentwise α low while maximizing the power of the test – Power refers to the ability of a statistical test to pick up true differences

between population means

• Researchers use many different post-hoc tests tailored to particular families of comparisons. Most of these tests are based on the t-test

• We will cover two such tests: – Fisher’s LSD (protected t-test)

– The Bonferroni procedure

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Intro to ANOVA

Fisher’s Least Significant Difference (LSD) Test

• Fisher’s LSD (protected t) test was the first proposed method for post-hoc pairwise comparisons

• It is nearly identical to the independent measures t-test. The only differences are that the denominator uses MSerror in place of pooled variance and uses dferror as the degrees of freedom for the t-statistic

• The t is “protected” in that the omnibus null hypothesis must be rejected for this test to be valid – The test is fairly liberal, producing higher than intended experimentwise α

for post-hoc tests involving more than 3 pairwise comparisons

2

21

1error

error error

t dfMS MS

n

M M

n

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Intro to ANOVA

The Bonferroni Procedure

• The Bonferroni procedure simply adjusts the pairwise alpha

for a group of comparisons to ensure that, in the worst case

scenario, the experimentwise alpha will never exceed 0.05

• The worst case occurs when the rejection of H0 under

different pairwise comparisons is mutually exclusive

• In this case, via the additive rule, the probability of falsely

rejecting H0 in k comparisons is α1+…+αk = kα

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Intro to ANOVA

The Bonferroni Procedure

• Thus, the Bonferroni procedure requires that you divide the

pairwise alpha by the number of comparisons.

– For example, if you wanted to make 10 pairwise comparisons at a

desired experimentwise α of 0.05, you would choose the rejection

region using a pairwise criterion of α/10 =0.005

• The Bonferroni procedure is a very conservative test. It is

guaranteed to keep the experimentwise Type I error rate

below α but is more likely to lead to Type II errors (acceptance

of H0 when it is false).

• The formula for the Bonferroni procedure is exactly like that

for Fisher’s LSD test.

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Intro to ANOVA

Post hoc tests: Example (Fisher’s LSD)

ANOVA Summary Table

2

1

3

3.43

3.3

2.98

M

M

M

Let’s do all possible comparisons: {1,2},{1,3},{2,3}

error

error e

A B

B

rror

A

M Mt df

MS MS

n n

First, note that the denominator is the same for all

comparisons:

1 2 3 10nn n

t-statistic for Fisher’s LSD test

when comparing {A,B}:

270.1750.153 0.153 0.0306

10 10

A B A B A BM M M M Mt

M

Source df SS MS F

Between 2 1.07 0.535 3.50

Within (error) 27 4.12 0.153

Total 29 5.19

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Post hoc tests: Example (Fisher’s LSD)

Let’s do all possible comparisons: {1,2},{1,3},{2,3}

Now we simply apply this formula to all comparisons:

1 2270.175

3.3

0.175

3.43

0.

0.1750.7

134

tM M

{1,2} {1,3} {2,3}

1 3270.175

2.98

0.175

3.43

0.

0.

452.571

175

Mt

M

2 327

0.175

3.3 2.98

0.17

0

5

0.175

.32.0

5

tM M

2

1

3

3.43

3.3

2.98

M

M

M

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Post hoc tests: Example (Bonferroni)

Let’s do all possible comparisons: {1,2},{1,3},{2,3}

1 2270.175

3.3

0.175

3.43

0.

0.1750.7

134

tM M

{1,2} {1,3} {2,3}

1 3270.175

2.98

0.175

3.43

0.

0.

452.571

175

Mt

M

2 327

0.175

3.3 2.98

0.17

0

5

0.175

.32.0

5

tM M

2

1

3

3.43

3.3

2.98

M

M

M

We have three comparisons, so the Bonferroni correction to α

would be 0.05

.017# 3comparisons

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Intro to ANOVA

Level of significance for one-tailed test

0.25 0.2 0.15 0.1 0.05 0.025 0.01 0.005 0.0005

Level of significance for two-tailed test

df 0.5 0.4 0.3 0.2 0.1 0.05 0.02 0.01 0.001

1 1.000 1.376 1.963 3.078 6.314 12.706 31.821 63.657 636.619

2 0.816 1.061 1.386 1.886 2.920 4.303 6.965 9.925 31.599

3 0.765 0.978 1.250 1.638 2.353 3.182 4.541 5.841 12.924

4 0.741 0.941 1.190 1.533 2.132 2.776 3.747 4.604 8.610

5 0.727 0.920 1.156 1.476 2.015 2.571 3.365 4.032 6.869

6 0.718 0.906 1.134 1.440 1.943 2.447 3.143 3.707 5.959

7 0.711 0.896 1.119 1.415 1.895 2.365 2.998 3.499 5.408

8 0.706 0.889 1.108 1.397 1.860 2.306 2.896 3.355 5.041

9 0.703 0.883 1.100 1.383 1.833 2.262 2.821 3.250 4.781

10 0.700 0.879 1.093 1.372 1.812 2.228 2.764 3.169 4.587

11 0.697 0.876 1.088 1.363 1.796 2.201 2.718 3.106 4.437

12 0.695 0.873 1.083 1.356 1.782 2.179 2.681 3.055 4.318

13 0.694 0.870 1.079 1.350 1.771 2.160 2.650 3.012 4.221

14 0.692 0.868 1.076 1.345 1.761 2.145 2.624 2.977 4.140

15 0.691 0.866 1.074 1.341 1.753 2.131 2.602 2.947 4.073

16 0.690 0.865 1.071 1.337 1.746 2.120 2.583 2.921 4.015

17 0.689 0.863 1.069 1.333 1.740 2.110 2.567 2.898 3.965

18 0.688 0.862 1.067 1.330 1.734 2.101 2.552 2.878 3.922

19 0.688 0.861 1.066 1.328 1.729 2.093 2.539 2.861 3.883

20 0.687 0.860 1.064 1.325 1.725 2.086 2.528 2.845 3.850 21 0.686 0.859 1.063 1.323 1.721 2.080 2.518 2.831 3.819

22 0.686 0.858 1.061 1.321 1.717 2.074 2.508 2.819 3.792

23 0.685 0.858 1.060 1.319 1.714 2.069 2.500 2.807 3.768

24 0.685 0.857 1.059 1.318 1.711 2.064 2.492 2.797 3.745

25 0.684 0.856 1.058 1.316 1.708 2.060 2.485 2.787 3.725 26 0.684 0.856 1.058 1.315 1.706 2.056 2.479 2.779 3.707

27 0.684 0.855 1.057 1.314 1.703 2.052 2.473 2.771 3.690

28 0.683 0.855 1.056 1.313 1.701 2.048 2.467 2.763 3.674

29 0.683 0.854 1.055 1.311 1.699 2.045 2.462 2.756 3.659

30 0.683 0.854 1.055 1.310 1.697 2.042 2.457 2.750 3.646 40 0.681 0.851 1.050 1.303 1.684 2.021 2.423 2.704 3.551

50 0.679 0.849 1.047 1.299 1.676 2.009 2.403 2.678 3.496

100 0.677 0.845 1.042 1.290 1.660 1.984 2.364 2.626 3.390

t-Distribution Table

Two-tailed test

One-tailed test

α

t

α/2 α/2

t -t

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01:830:200:01-05 Fall 2014

Intro to ANOVA

Post hoc tests: Example

Let’s do all possible comparisons: {1,2},{1,3},{2,3}

Now we simply apply this formula to all comparisons:

1 2270.175

3.3

0.175

3.43

0.

0.1750.7

134

tM M

{1,2} {1,3} {2,3}

00.74 2.052, retai n H 02.571 2.052, rejec t H0 r2.0 eta2.052, in H

1 3270.175

2.98

0.175

3.43

0.

0.

452.571

175

Mt

M

2 327

0.175

3.3 2.98

0.17

0

5

0.175

.32.0

5

tM M

2

1

3

3.43

3.3

2.98

M

M

M

Fisher’s LSD:

Bonferroni: 00.74 2.473, retai n H 02.571 2.473, rejec t H0 r2.0 eta2.473, in H