Investigation of a Unilateral Pleural Effusion in Adults 2

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Investigation of a unilateral pleural effusion in adults:British Thoracic Society pleural diseaseguideline 2010

Clare Hooper,1 Y C Gary Lee,2 Nick Maskell,3 on behalf of the BTS Pleural Guideline

Group

INTRODUCTIONPleural effusions are a common medical problemwith more than 50 recognised causes includingdisease local to the pleura or underlying lung,systemic conditions, organ dysfunction and drugs.1

Pleural effusions occur as a result of increaseduid formation and/or reduced uid resorption.The precise pathophysiology of uid accumulationvaries according to underlying aetiologies. As thedifferential diagnosis for a unilateral pleural effu-sion is wide, a systematic approach to investigationis necessary. The aim is to establish a diagnosisswiftly while minimising unnecessary invasiveinvestigations and facilitating treatment, avoidingthe need for repeated therapeutic aspirations whenpossible.

Since the 2003 guideline, several clinically rele-vant studies have been published, allowing newrecommendations regarding image guidance of pleural procedures with clear benets to patientcomfort and safety, optimum pleural uid samplingand processing and the particular value of thor-acoscopic pleural biopsies. This guideline alsoincludes a review of recent evidence for the use of

new biomarkers including N-terminal pro-brainnatriuretic peptide (NT-proBNP), mesothelin andsurrogate markers of tuberculous pleuritis.

CLINICAL ASSESSMENT AND HISTORY < Aspiration should not be performed for

bilateral effusions in a clinical settingstrongly suggestive of a transudate unlessthere are atypical features or they fail torespond to therapy. (U)

< An accurate drug history should be takenduring clinical assessment. (U)

The history and physical examination of a patient

with a pleural effusion may guide the clinician as towhether the effusion is a transudate or an exudate.This critical distinction narrows the differentialdiagnosis and directs further investigation.

Clinical assessment alone is often capable of identifying transudative effusions. Therefore, in anappropriate clinical setting such as left ventricularfailure with a conrmatory chest x-ray, sucheffusions do not need to be sampled unless thereare atypical features or they fail to respond totreatment.

Approximately 75% of patients with pulmonary embolism and pleural effusion have a history of pleuritic pain. These effusions tend to occupy less

than one-third of the hemithorax and the dyspnoea

is often out of proportion to the size of the effu-sion.2 3 As tests on the pleural uid are unhelpfulin diagnosing pulmonary embolism, a high indexof suspicion is required to avoid missing thediagnosis.

The patient’s drug history is also important. Although uncommon, a number of medicationshave been reported to cause exudative pleuraleffusions (box 1). Useful resources for more detailedinformation include the British National Formularyand the web site http://www.pneumotox.com/.

An occupational history including details aboutknown or suspected asbestos exposure and poten-tial secondary exposure via parents or spousesshould be documented. An algorithm for theiinvestigation of a unilateral pleural effusion isshown in gure 1.

INITIAL DIAGNOSTIC IMAGINGPlain radiography < Posteroanterior (PA) chest x-rays should be

performed in the assessment of suspectedpleural effusion. (U)

The plain chest radiographic features of pleuraleffusion are usually characteristic. The poster-oanterior (PA) chest x-ray is abnormal in the pres-ence of about 200 ml of pleural uid. However, only 50 ml of pleural uid can produce detectableposterior costophrenic angle blunting on a lateralchest x-ray.4

In the intensive care setting, most chest x-raysare performed as AP supine examinations, resultingin free pleural uid lying posteriorly in the depen-dent portion of the chest. Consequently, effusionsare seen as an increase in hemithorax opacity withpreserved vascular shadows on the supine x-ray.Other signs include the loss of the sharp silhouette

of the ipsilateral hemidiaphragm and uid tracking

Box 1 Commonly prescribed drugs known to

cause pleural effusions (over 100 cases

reported globally)

< Methotrexate< Amiodarone< Phenytoin< Nitrofurantoin< b-blockersSource: http://www.pneumotox.com (2009)

1Southmead Hospital, Bristol,UK2Lung Institute of WesternAustralia, University Dept Med,Perth, Western Australia,Australia3Department of ClinicalSciences, Southmead Hospital,University of Bristol, Bristol, UK

Correspondence toNick Maskell, AcademicRespiratory Unit, Department of

Clinical Sciences, SouthmeadHospital, University of Bristol,BS10 5NB;[email protected]

Received 12 February 2010Accepted 4 March 2010

ii4 Thorax 2010;65(Suppl 2):ii4eii17. doi:10.1136/thx.2010.136978

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down into the oblique or horizontal ssures resulting inapparent ssural thickening. The volume of pleural uid iscommonly underestimated on a supine chest x-ray and ‘normal’

appearances do not exclude the presence of an effusion. 5

Subpulmonic effusions occur when pleural uid accumulatesbetween the diaphragmatic surface of the lung and thediaphragm. They are often transudates, can be dif cult todiagnose on the PA lm and may require an ultrasound scan. ThePA lm will often show a lateral peaking of an apparently raisedhemidiaphragm which has a steep lateral slope with a gradualmedial slope (see gure 2). The lateral x-ray may have a atappearance of the posterior aspect of the hemidiaphragm witha steep downward slope at the major ssure.6

Ultrasound< Bedside ultrasound guidance signicantly increases the

likelihood of successful pleural

uid aspiration andreduces the risk of organ puncture. (B)

< Ultrasound detects pleural uid septations with greatersensitivity than CT. (C)

Ultrasound guidance improves the rate of successful pleural

aspiration. Several studies have shown that uid can besuccessfully obtained using ultrasound in up to 88% of patientsafter a failed clinical and plain chest x-ray-guided attempt.7e9

Ultrasound guidance reduces the incidence of iatrogenicpneumothorax following thoracentesis and several studies haveshown this effect to be independent of the size of theeffusion.10 11

This benet appears to be lost when the ‘X marks the spot’method is employed, presumably due to differences in patientpositioning between the ultrasound and the procedure.12

Clinical judgement with review of the chest x-ray wascompared with ultrasonography in planning the diagnosticaspiration site in a prospective study including 255 clinicianassessments of 67 patients.4 The sensitivity and specicity of

clinical judgement compared with the gold standard of ultra-sound was 76.6% and 60.3%, respectively. Ultrasound increased

Figure 1 Diagnostic algorithm for theinvestigation of a unilateral pleuraleffusion.

History, clinical examination & CXR

Does the clinical picture suggest a transudate?

e.g. LVF, hypoalbuminaemia, dialysis

Has the fluid

analysis and

clinical features

given a diagnosis?

Refer to a chest physician

Is it a

transudate?

Resolved? STOP

Diagnostic algorithm for the investigation of a unilateral pleural effusion

Treat

appropriately

YES

NO

NO

YES

NO

YES

Cause found?

NO

Re-consider treatable conditions such as PE,

TB, chronic heart failure and lymphoma.

Wa tchful waiting often appropriate.

NO

Treat

the cause

YES

Treat

the cause

YES

Pleural aspiration (with ultrasound guidance)

Send for: cytology, protein, LDH, pH

Gram stain, culture and sensitivity.

(Additional tests if warranted - see text box)

NO

Request contrast enhanced CT thorax.

Treat

appropriately

Consider LA thoracoscopy

or surgical VATS

Consider radiological guided

pleural biopsy

+/- chest tube drainage if

symptomatic

Thorax 2010;65(Suppl 2):ii4eii17. doi:10.1136/thx.2010.136978 ii5

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the number of accurate sites by 26%; 15% of clinically deter-mined sites would have resulted in the puncture of liver, spleenor lung and, although there was increasing risk with small orloculated effusions, 60% of potential organ punctures occurredin radiologically large or moderate effusions.

Ultrasound is superior to plain radiography in diagnosing andquantifying pleural effusions and distinguishes pleural uid fromthickening with high specicity, particularly when colourDoppler is employed.13e16 It is particularly useful in the diag-nosis of small effusions or in recumbent patients (eg, ventilatedand critically ill) due to the low sensitivity of plain radiography in these situations.

The diagnostic role of thoracic ultrasound in the early inves-tigation of pleural effusions extends beyond the identicationand safe aspiration of uid.

Ultrasound detects septations within pleural uid withgreater sensitivity than CT scanning.17 A septated appearancemay be observed in malignant effusions or pleural infection andoccurs with similar frequency in the two diagnoses.18

Ultrasound positively identies exudative effusions whenpleural uid is complex, septated or echogenic, although simple(anechoic) effusions can be exudates or transudates.19

Ultrasound features can distinguish malignant from benigneffusions. Qureshi et al demonstrated 95% specicity for

a malignant diagnosis, 95% for parietal pleural thickening>1 cm, 100% for visceral pleural thickening, 95% for diaphrag-matic thickening >7 mm and 100% for diaphragmatic nodulesas visualised on ultrasound examination.20 Overall sensitivity of ultrasound in the differentiation of malignant from benigneffusions was 79% (95% CI 61% to 91%) and specicity of 100%(95% CI 82% to 100%), with specicity comparing favourably with CT scanning (89%).

PLEURAL ASPIRATION< A diagnostic pleural uid sample should be aspirated

with a ne-bore (21G) needle and a 50 ml syringe. (U)< Bedside ultrasound guidance improves the success rate

and reduces complications (including pneumothorax)and is therefore recommended for diagnostic aspira-tions. (B)

< Pleural uid should always be sent for protein, lactatedehydrogenase, Gram stain, cytology and microbiolog-ical culture. (C)

This is the primary means of evaluating pleural uid and itsndings are used to guide further investigation.

Pleural ultrasound should be used at the bedside to selecta pleural aspiration site with safety. Ultrasound increases thechances of successful aspiration and minimises the need forrepeated attempts.21 Direct ultrasound-guided aspiration orultrasound at the bedside immediately before the procedure ispreferable to the ‘X marks the spot’ approach. A lateral site ispreferred, provided that adequate uid is demonstrated here onultrasound as the risk of intercostal vessel trauma increases withmore posterior or medial punctures (see gure 3).

Patient consent and further technical details of pleuralaspiration are covered in the guideline on pleural procedures.Table 1 shows sample collection guidance for specic pleuraluid tests.

A green needle (21G) and 50 ml syringe are adequate for diag-

nostic pleural aspirations. If there is diagnostic suspicion of pleural infection and a pleural uid pH is to be measured, aspi-rated uid should immediately be drawn into a heparinised bloodgas syringe which should then be capped while awaiting analysisto avoid exposure of the uid to the air. The remaining sampleshould be divided between sample pots for microbiological (5 ml),biochemical (2e5 ml) and cytological (remaining sample whichshould be 20e40 ml) analysis. Microscopic examination of Gram-stained pleural uid sediment is necessary for all pleural uidsamples. If infection is suspected, some of the pleural uid shouldbe sent in blood culture bottles which increases diagnosticaccuracy, particularly for anaerobic organisms.22

Figure 2 Chest x-ray showing a moderate left pleural effusion andsubpulmonic effusion on the right (a). Note the lateral peaking of theright hemidiaphragm. Reproduced with permission from Professor David

Milne, Auckland University.

Figure 3 CT scan (A) before and (B)2 days later after a pleural aspirationwith inappropriate medial approach andintercostal artery puncture with resultanthaemothorax requiring surgicalintervention. Note the active bleedingindicated by the arrow.


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There is conicting evidence regarding the optimum volumeof pleural uid for diagnosis of malignancy; sensitivity dependson the cellularity of the sample and processing technique as wellas volume submitted.23 24 It is sensible to send as large a volumeas possible from the 50e60 ml sample obtained following diag-nostic aspiration as other tests only require small volumes. Atroom temperature the sample for cytology should be sent to thelaboratory as quickly as possible but, if a delay is anticipated, thespecimen can be refrigerated at 48C for up to 14 days with nodeterioration in the diagnostic yield for malignancy (table 1).25

Appearance

< The appearance of the pleural uid and any odourshould be recorded. (U)

< A pleural uid haematocrit is helpful in the diagnosis of haemothorax. (U)

Table 2 summarises the appearance of pleural effusions due tospecic causes. Fluid may appear serous, blood-tinged, frankly

bloody or purulent. Centrifuging turbid or milky pleural uidwill distinguish between empyema and lipid effusions. If thesupernatant is clear, the turbid uid was due to cell debris andempyema is likely while, if it is still turbid, chylothorax orpseudochylothorax are likely.26 The unpleasant smell of anaer-obic infection may guide antibiotic choices and the smell of ammonia suggests urinothorax.

Grossly bloody pleural uid is usually due to malignancy,pulmonary embolus with infarction, trauma, benign asbestospleural effusions or post-cardiac injury syndrome.27 28

A haemothorax can be distinguished from other blood-stainedeffusions by performing a haematocrit on the pleural uid. A pleural uid haematocrit >50% of the patient’s peripheral bloodhaematocrit is diagnostic of a haemothorax.29

Differentiating between a pleural fluid exudate and transudate< Light’s criteria should be used to distinguish between

a pleural uid exudate and transudate (box 2). (B)< In order to apply Light’s criteria, the total protein and

lactate dehydrogenase (LDH) should be measured inboth blood and pleural uid. (B)

Categorisation of pleural effusions into transudates and

exudates is an important early step in narrowing the differentialdiagnosis and directing subsequent investigations and manage-ment (see boxes 3 and 4).

Classically, pleural uid protein >30 g/l has indicated anexudate and 0.5< Pleural fluid lactate dehydrogenase (LDH) divided by serum

LDH is >0.6

< Pleural fluid LDH >2/3 the upper limits of laboratory normalvalue for serum LDH.

Box 3 Causes of pleural transudates

Very common causes< Left ventricular failure< Liver cirrhosisLess common causes< Hypoalbuminaemia< Peritoneal dialysis< Hypothyroidism< Nephrotic syndrome< Mitral stenosisRare causes< Constrictive pericarditis< Urinothorax<

Meigs’ syndrome

Table 2 Diagnostically useful pleural fluid characteristics

Fluid Suspected disease

Putrid odour Anaerobic empyema

Food particles Oesophageal rupture

Bile stained Cholothorax (biliary fistula)

Milky Chylothorax/pseudochylothorax

‘Anchovy sauce’ like fluid Ruptured amoebic abscess


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accuracy of 93e

96%, remains a robust method.31 32 This discrim-inatory accuracy is unlikely to be surpassed as the ‘gold standard’forcomparison in clinical diagnosis which itself carries anerrorrate.

In congestive cardiac failure, diuretic therapy increases theconcentration of protein, lactate dehydrogenase (LDH) andlipids in pleural uid and, in this context, Light’s criteria arerecognised to misclassify a signicant proportion of effusions asexudates.33 34

Although the use of continuous likelihood ratios rather thana dichotomous division of transudates versus exudates has beenproposed, particularly to overcome loss of accuracy of Light’scriteria when pleural protein and LDH levels are close to cut-off values, there is probably little value in this cumbersome statis-

tical method beyond careful interpretation of test results in thelight of clinical judgement.35

N-terminal pro-brain natriuretic peptide (NT-proBNP)NT-proBNP is a sensitive marker of both systolic and diastoliccardiac failure. Levels in blood and pleural uid correlate closely and measurement of both has been shown in several series to beeffective in discriminating transudates associated with conges-tive heart failure from other transudative or exudativecauses.36 e39 The cut-off value of these studies, however, variedwidely from 600 to 4000 pg/ml (with 1500 pg/ml being mostcommonly used), and most studies excluded patients with morethan one possible aetiology for their effusion. NT-proBNP hasbeen shown to correctly diagnose congestive heart failure as

a cause of most effusions that have been misclassied asexudates by Light’s criteria. Use of this test may therefore avoidrepeated invasive investigations in patients where there isa strong clinical suspicion of cardiac failure.40e42 As results withpleural uid and blood are comparable, applying the test toblood alone is suf cient (see evidence table A available on theBTS website at www.brit-thoracic.org.uk).

Evidence for the use of measuring BNP (also known asC-terminal BNP, the active peptide from which NT-proBNP iscleaved) is relatively scarce to date.

Pleural fluid differential cell counts< Pleural uid cell proportions are helpful in narrowing

the differential diagnosis but none are disease-specic. (C)

< Any long-standing pleural effusion tends to becomepopulated by lymphocytes. Pleural malignancy, cardiacfailure and tuberculosis are common specic causes of

lymphocyte-predominant effusions. (C)If the pleural uid differential cell count shows a predominantlymphocytosis (>50% cells are lymphocytes), the most likely diagnoses worldwide are malignancy and tuberculosis (TB).43

Cardiac failure is also a common cause of a lymphocytic effu-sion. Very high lymphocyte proportions (>80%) occur mostfrequently in TB, lymphoma, chronic rheumatoid pleurisy,sarcoidosis and late post-coronary artery bypass grafting(CABG) effusions (see box 5).44

Neutrophil-predominant pleural effusions are associated withacute processes. They occur in parapneumonic effusions,pulmonary embolism, acute TB and benign asbestos pleuraleffusions.28 45

Pleural effusions in which $10% of cells are eosinophils aredened as eosinophilic.46 The most common cause of pleuraluid eosinophilia is air or blood in the pleural space.47 Pleuraleosinophilia is a relatively non-specic nding as it can occur inparapneumonic effusions, drug-induced pleurisy, benign asbestospleural effusions, ChurgeStrauss syndrome, lymphoma,pulmonary infarction and parasitic disease.4 8 4 9 Malignancy isalso a common cause; a malignant diagnosis was made in 37% of 60 eosinophilic effusions in one series.46

pH< In non-purulent effusions, when pleural infection is

suspected, pleural uid pH should be measuredproviding that appropriate collection technique can beobserved and a blood gas analyser is available. (B)

< Inclusion of air or local anaesthetic in samples maysignicantlyalterthepH results andshould be avoided.(B)

< In a parapneumonic effusion, a pH of


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In malignant pleural effusions low pH has been associatedwith shorter survival, more extensive disease and a lower chanceof successful pleurodesis.52 A meta-analysis including 417patients with malignant pleural effusions found that a pleuralpH 7.28.53

In clinical practice, the most important use for pleural uidpH is aiding the decision to treat pleural infection with tubedrainage. A meta-analysis of studies examining pleural pH andthe need for chest tube drainage or surgery in patients witha parapneumonic effusion found that a pH 7.2 ina patient with other clinical indicators of complicated pleuralinfection should be viewed with caution.55

The collection and analysis technique can have a clinically signicant impact on pleural uid pH results. A prospectivestudy found that exposure of uid to air in the syringe increasedthe measured pleural uid pH by $0.05 in 71% of samples andinclusion of 0.2 ml local anaesthetic produced a mean reductionin pH of 0.15 (95% CI 0.13 to 0.18).56 Pleural uid shouldtherefore be collected and transported without exposure toatmospheric air and local anaesthetic avoided for diagnosticaspirations where the pH will be used to guide management.Pleural pH does not change signicantly if processing is delayedfor up to an hour at room temperature. An arterial blood gasanalyser should be used.57 In routine clinical practice it is oftendif cult to adhere to these collection requirements and,when they cannot be achieved, overall clinical assessmentmay be preferable to reliance on a suboptimal pleural uid

pH result.

GlucoseIn the absence of pleural pathology, glucose diffuses freely acrossthe pleural membrane and the pleural uid glucose concentrationis equivalent to blood.1

A low pleural uid glucose level (50 ml did not improve thediagnostic yield.23 24 The evidence for sending large volumes of pleural uid is not strong enough to justify the increased risk of complications associated with the use of a venon and three-

way tap for initial diagnostic aspiration. As much uid aspossible should be sent for cytology from the available diag-nostic sample (likely to be 20e40 ml) and, when the initialresult is negative but malignancy is suspected, the sending of a higher volume sample following a second aspiration should beconsidered. If the initial aspiration is both therapeutic anddiagnostic, $60 ml should be sent for cytological examination.

Pleuraluid shouldbe sent in a plain container which allowsthecellular portion to separate, forming a brinous ‘clot’ which may enmesh malignant cells. These can then undergo histologicalexamination and are reported with the uid cytology. Somedepartments, however, prefer the use of bottles containing sodiumcitrate to keep the cells in free suspension. No other anticoagu-

lants or preservatives should be used as they may interferewith cellular adherence to slides and immunocytochemistry.


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The yield for malignancy increases if both cell blocks (whichare formed by centrifuging the sample and extracting the solidcellular portion) and smears are prepared from pleural uidsamples.71

Table 3 provides an interpretation of common pleural uidcytology reports seen in clinical practice.

Once malignancy has been conrmed morphologically,immunocytochemistry should be used to differentiate betweendifferent malignant cell types. This can be performed ona cytology sample, cell block or a clot.72 There is particularly extensive morphological overlap between malignant mesothe-lioma and metastatic adenocarcinoma cells and immunocyto-chemistry can assist in their differentiation. However, wheneverpossible, pleural tissue should be obtained to conrm a diagnosisof malignant mesothelioma.

If lymphoma is suspected on morphological examination,ideally a sample should be submitted for ow cytometry forfurther typing, but immunocytochemistry can be used if this isunavailable (table 3).73

TUMOUR MARKERS< Pleural uid and serum tumour markers do not

currently have a role in the routine investigation of pleural effusions. (C)

At a cut-off level that achieves 100% specicity for the diagnosisof malignancy, a panel of pleural uid tumour markers includingCEA, CA-125, CA 15-3 and CYFRA has been shown to reacha combined sensitivity of only 54%, such that a negative resultcannot be used to support a conservative approach to monitoringand investigation.74

Mesothelin, however, has been shown to have more promisingdiagnostic characteristics (see evidence table B available on theBTS website at www.brit-thoracic.org.uk).

MesothelinMesothelin is a glycoprotein tumour marker that is present athigher mean concentrations in the blood and pleural uid of patients with malignant mesothelioma than in patients withother causes of pleural effusion.7 5 7 6 Studies examining meso-thelin levels in serum and/or pleural uid have demonstrateda sensitivity of 48e84% and specicity of 70e100% for thediagnosis of mesothelioma.75e80 The negative predictive value of the test is limited by false negatives in sarcomatoid mesothe-lioma.79 Positive results have also been recognised in broncho-genic adenocarcinoma, metastatic pancreatic carcinoma,lymphoma and ovarian carcinoma.76 78 81

A positive serum or pleural uid mesothelin level is highly

suggestive of pleural malignancy and might be used to expeditea tissue diagnosis, but a negative result cannot be considered

reassuring. Pleural uid mesothelin has been shown to haveadditional value beyond pleural uid cytology in the diagnosis of mesothelioma and might be used for its positive predictive valueto clarify indeterminate cytology results.80 Although mesothelinhas a greater diagnostic accuracy than other tumour markers, itsreal clinical utility in the investigation of an undiagnosed pleuraleffusion, particularly in combination with routine clinical andradiological assessment, warrants further study before its use

can be routinely recommended.

FURTHER DIAGNOSTIC IMAGINGComputed tomography (CT)< CT scans for pleural effusion should be performed with

contrast enhancement of the pleura and beforecomplete drainage of pleural uid. (C)

< CT scans should be performed in the investigation of allundiagnosed exudative pleural effusions and can be useful indistinguishing malignant from benign pleural thickening. (C)

< A CT scan should be requested for complicated pleuralinfection when initial tube drainage has been unsuc-cessful and surgery is to be considered. (C)

When investigating a pleural effusion, a contrast-enhancedthoracic CT scan should be performed before full drainage of theuid as pleural abnormalities will be better visualised.82 Free-owing pleural uid is seen as a sickle-shaped opacity in themost dependent part of the thorax. Suspended air bubbleswithin the uid imply septations (gure 4), but CT does notdistinguish the internal characteristics of pleural uid with thesame sensitivity as ultrasound.17

CT is particularly helpful in the diagnosis of empyema whenthe pleura enhances intensely around the uid which usually forms a lenticular opacity (gure 4).83 84 CT also distinguishesempyemas from lung abcesses.

There are features of contrast-enhanced thoracic CT scanningwhich can help differentiate between benign and malignant

disease (gure 5). In a study of 74 patients, 39 of whom hadmalignant disease, Leung et al showed that malignant disease isfavoured by nodular pleural thickening, mediastinal pleuralthickening, parietal pleural thickening >1 cm and circumferen-tial pleural thickening. These features had specicities of 94%,94%, 88% and 100%, respectively, and sensitivities of 51%, 36%,56% and 41%.85 The accuracy of the criteria of Leung et al for thedetection of pleural malignancy has been conrmed in severalprospective studies.82 86 Differentiation of pleural mesothelioma

Table 3 Reporting of pleural fluid cytology results

Report Interpretation

Inadequate No mesothelial cells or only degeneratecells present

No mal ig nant ce lls seen Ad eq uate samp le witho ut e vidence ofmalignancy (does not excludemalignancy)

Atypical cells May be of inflammatory or malignantorigin. Sending a further sample may be

helpful

Suspicious for malignancy Occasional cells with malignant featuresbut not definitively malignant

Malignant Unequivocal malignant cells presentwhich require typing byimmunocytochemistry

Figure 4 CT scan of left empyema with pleural enhancement (a) andsuspended air bubbles (b).


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from metastatic pleural malignancy is very dif cult as the

conditions share many CT features.

Magnetic resonance imaging (MRI)MRI distinguishes accurately between benign and malignantpleural effusions via differences in signal intensity onT2-weighted images.87 Distinction of morphological features of pleural malignancy by MRI has been shown in some studies toequal CT and assessment of diaphragmatic and chest wallinvolvement is superior.88 Access to MRI is limited and it doesnot have a place in the routine investigation of pleural effusionsat this time, but may be used to accurately assess pleural diseasein patients for whom contrast is contraindicated. Dynamiccontrast-enhanced MRI has shown promise in the monitoring of response of pleural mesothelioma to chemotherapy.89

PET-CT imaging While the uptake of 18-uorodeoxyglucose (FDG) has beenshownto be greater in malignant pleural effusions, the value of PET-CTimaging in distinguishing benign and malignant disease is limitedby false positives in patients with pleural inammation includingpleural infection and following talc pleurodesis.90e92 PET-CTimaging does not currently have a role in the routine investigationof pleural effusions but, in common with dynamic contrast-enhanced MRI, there is emerging evidence suggesting a potentialrole in monitoring the response to treatment of pleuralmesothelioma.93e95

INVASIVE INVESTIGATIONSPercutaneous pleural biopsy < When investigating an undiagnosed effusion where

malignancy is suspected and areas of pleural nodularityare shown on contrast-enhanced CT, an image-guidedcutting needle is the percutaneous pleural biopsymethod of choice. (A)

< Abrams needle biopsies are only diagnostically useful inareas with a high incidence of TB, although thoraco-scopic and image-guided cutting needles have beenshown to have a higher diagnostic yield. (C)

A review of Abrams pleural biopsy yield from 2893 examinationsshowed a diagnostic rate of only 57% for malignancy.96 The

yield over pleural

uid cytology alone is increased by only 7e27% for malignancy.6 8 6 9 Complications of Abrams pleural

biopsy include site pain (1e15%), pneumothorax (3e15%),vasovagal reaction (1e5%), haemothorax (


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0.40% (95% CI 0.2% to 0.7%). It has a diagnostic sensitivity formalignant pleural disease of 92.6% (95% CI 91.0% to93.9%).101e121 It also has a higher diagnostic yield than blindpleural biopsy for tuberculous pleuritis. Talc poudrage can beadministered at the end of the procedure which achievesa successful pleurodesis in 80e90% (see BTS guideline onthoracoscopy for further detail.

Video-assisted thoracoscopic surgery (VATS)This is performed by thoracic surgeons and requires a generalanaesthetic. It is therefore not a suitable option for frail indi-viduals and those with other severe comorbidities. This proce-dure reports similarly high diagnostic sensitivity rates of approximately 95% for malignancy and is also relatively safewith a low complication rate. In one series of 566 examinations,the most common side effect was subcutaneous emphysemawith cardiac dysrhythmia and air embolism occurring in


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rheumatoid arthritis, this makes other causes for the effusionunlikely. Rheumatoid arthritis is unlikely to be the cause of a chronic effusion if the glucose level in the uid is >1.6 mmol/l,serving as a useful screening test.58 80% of rheumatoid pleuraleffusions have a pleural uid glucose to serum ratio of 1.24 mmol/l (110 mg/dl)

Cholesterol >5.18 mmol/l (200 mg/dl) Usually low

Cholesterol crystals Often present Absent

Chylomicrons Absent Usually present


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Benign asbestos pleural effusionBenign asbestos pleural effusions are commonly diagnosed inthe rst two decades after asbestos exposure. The prevalenceis dose-related with a shorter latency period than otherasbestos-related disorders.155 The effusion is usually small andasymptomatic, often with pleural uid which is haemor-rhagic.156 157 There is a propensity for the effusion to resolvewithin 6 months, leaving behind residual diffuse pleural thick-ening.156 157 As there are no denitive tests, the diagnosiscan only be made with certainty after a prolonged period of

follow-up and consideration should be given to early thoraco-scopy with pleural biopsy in any patient with a pleural effusionand a history of asbestos exposure, particularly in the presenceof chest pain. Table 5 summarises clinical and pleural uidcharacteristics of other important causes of unilateral pleuraleffusions.

MANAGEMENT OF PERSISTENT UNDIAGNOSED EFFUSIONSEven after a complete investigation including thoracoscopicbiopsies, a signicant number of patients with pleural exudatesare diagnosed with ‘non-specic pleuritis’ and no specic diag-nosis can be made. A retrospective study of 75 such patientsfound that only 8.3% of these turned out to be malignant overa 2-year follow-up period. The majority of patients withnon-specic pleuritis (91.7%) followed a benign course, withspontaneous resolution of the effusion in 81.8% of cases.163

In patients not t enough for thoracoscopy, it is sensible toreconsider diagnoses with a specic treatment (eg, TB, pulmo-nary embolism, lymphoma and chronic heart failure). A considerable number of undiagnosed pleural effusions in thiscategory are due to a malignant process. Watchful waiting may be the appropriate management in this setting.

Competing interests No member of the Guideline Group is aware of any competinginterests.

Provenance and peer review The draft guideline was available for online publicconsultation (July/August 2009) and presented to the BTS Winter Meeting (December2009). Feedback was invited from a range of stakeholder institutions (see

Introduction). The draft guideline was reviewed by the BTS Standards of CareCommittee (September 2009).

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Table 5 Other important causes of pleural effusions

Condition Clinical features Pleural fluid characteristics Special investigations and management

Early post-CABG pleural effusion158 Occur within 30 days of CABG.Left > right.Most small and asymptomatic.Prevalence 89% at 7 days postoperatively

Exudate.Bloody (haematocrit >5%).Often eosinophilic

Only perform diagnostic aspiration if thepatient is febrile, complains of pleuriticchest pain or the effusion is very large.Most settle spontaneously

Late post-CABG pleural effusion159 Occur >30 days post-CABG.Left > right.May be large and associated with

dyspnoea

Exudate.Clear/yellow.Lymphocytic

Diagnostic aspiration to exclude othercauses and confirm the diagnosis.Repeated therapeutic thoracentesis

usually successful for symptomaticeffusions.

Urinothorax160 Due to obstructive uropathy.Urine tracks through the retroperitoneumto the pleural space.

Pleural fluid creatinine > serumcreatinine.Transudate.Low pH

Usually resolves with relief of the renalobstruction

Ovarian hyperstimulation syndrome161 Life-threatening reaction to ovulationinduction (hCG or clomiphene).May be pleural effusion alone (usuallyright sided) or whole syndrome with:massive ascites, renal and hepatic failure,thromboemboli and ARDS

Exudate with both protein and LDH inexudative range

Repeated therapeutic aspirations oftenrequired to relieve dyspnoea

Lymphoma-related pleural effusion162 Effusion may be associated withmediastinal lymphadenopathy on CT butoften there are no clinical features todistinguish from other causes of pleuraleffusion

Exudate.Lymphocytic.Positive cytology in around 40%.Chylothorax in around 15%

Pleural fluid flow cytometry andcytogenetics may be useful.Thoracoscopic pleural biopsies are oftennegative but required to exclude othercauses if diagnosis unclear

ARDS, adult respiratory distress syndrome; CABG, coronary artery bypass graft; hCG, human chorionic gonadotrophin.


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disease guideline 2010in adults: British Thoracic Society pleuralInvestigation of a unilateral pleural effusion

Clare Hooper, Y C Gary Lee and Nick Maskell

doi: 10.1136/thx.2010.1369782010 65: ii4-ii17Thorax

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