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List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 1
List of control strains for performance testing of culture media and reagents from
published standards from ISO/TC 34/SC 9 & SC 5 (food microbiology)
and ISO/TC 147/SC 4 (water microbiology)
Content
Table 1: Change history of the document editions page 1
Table 2: Footnotes page 2
Table 3: Control strains for culture media and reagents
from ISO/TC 34/SC 9 & SC 5 standards (food microbiology) page 3 - 26
Table 4: Control strains for culture media and reagents
from ISO/TC 147/SC 4 standards (water microbiology) page 27 – 35
Table 1 — Change history of the document
Edition Changes Published by First Initiation 2020-10-12
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 2
Table 2 — Footnotes used in Table 3 and Table 4
a Make reference to the reference strain catalogue available on http://www.wfcc.info for information
on culture collection strain numbers and contact details. b Strain to be used as a minimum. c Some national restrictions and directions may require the use of a different serovar. Make
reference to national requirements relating to the choice of Salmonella serovars. d Strain free of choice; one of the strains has to be used as a minimum. e Strain free of choice; one of the positive strains has to be used as a minimum for a positive reaction. f Strain free of choice; one of the negative strains has to be used as a minimum for a negative
reactions. g Strain optional. h Non-toxigenic strain of E. coli serotype O157. i If nutrient agar is used for two or three of these different applications: perform the Salmonella
growth test as a minimum (if laboratory tests for this organism). j If BPW is used for more than one application, perform at least one enrichment test as a minimum
using a pathogenic organism appropriate to the laboratory’s range of test methods, e.g. Salmonella. k Weak coagulaseproducing strain of Staphylococcus aureus. l Escherichia coli WDCM 00013 is a strong β-d-glucuronidase producer and WDCM 00202 is a weak
β-d-glucuronidase producer. m Escherichia coli WDCM 00013 is given by the specific standard. n Some national restrictions and directions may require the use of a different E. coli serovar. Make
reference to national requirements relating to the choice of E. coli serovars. o Choose the strain(s) according to the method for which TSA is used as a reference medium.
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 3
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Agar Listeria according to Ottaviani and Agosti
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11290-1:2017 ISO 11290-2:2017
Listeria monocytogenes serovar 4b
00021b
Listeria monocytogenes serovar 1/2a
00109g
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Listeria innocua 00017b
α-Glucosidase enzymatic assay solution
ISO 22964:2017 - - -
Alkaline saline peptone water (ASPW)
ISO 21872-1:2017 - - -
Arginine dihydrolase saline medium (ADH)
ISO 21872-1:2017 ISO 11133:2014 / Amd 2:2020 Vibrio fluvialis 00137e
Vibrio parahaemolyticus 00037f / 00185f
Baird-Parker agar medium (Baird-Parker)
ISO 6888-1:1999 ISO 11133:2014
Staphylococcus aureus 00034b / 00032g
Escherichia coli 00012d / 00013d
Staphylococcus saprophyticus 00159b
Staphylococcus epidermidis 00036g
BCP (Bromocresol purple) plate count skim milk agar with 0,2 % mass fraction starch
ISO/TS 27265:2009 - - -
Bile and esculin agar ISO 10273:2017 ISO 10273:2017 Yersinia intermedia 00217e
Yersinia enterocolitica 00216f / 00160f
Bismuth sulfite (BS) agar ISO 6579-1:2017 ISO 6579-1:2017 / Amd 1:2020
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 4
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Blood agar (sheep, calf or bovine) for haemolysis reaction
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11133:2014 / Amd 2:2020 ISO 11290-1:2017 ISO 11290-2:2017
Listeria monocytogenes 00021e / 00109e
Listeria innocua 00017f
Blood agar (reference medium)
ISO 10272-2:2017
ISO 11133:2014 Campylobacter jejuni 00156d / 00005d
Campylobacter coli 00004d
Bolton broth ISO 10272-1:2017 ISO 10272-1:2017
Camplyobacter jejuni 00156d / 00005d
Campylobacter coli 00004g
Escherichia coli 00012d / 00013d
Proteus mirabilis 00023b
Brain heart infusion (BHI) broth
ISO 6888-1:1999 ISO 6888-3:2003
ISO 11133:2014 Staphylococcus aureus 00034b
Brain heart infusion broth ISO 8870:2006 - - -
Brillant green lactose bile (BGBLB) broth
ISO 4831:2006 ISO 4832:2006
ISO 11133:2014 / Amd 2:2020 Escherichia coli
00012d / 00013d / 00090d / 00179d
Enterococcus faecalis 00009d / 00087d /00176d
Buffered peptone water (BPW) (pre-enrichment)i
ISO 6579-1:2017 ISO 6579-1:2017 Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Buffered peptone water (BPW) (pre-enrichment)i
ISO/TS 6579-2:2012 ISO 11133:2014 Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 5
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Buffered peptone water (BPW) (pre-enrichment)i
ISO 21528-1:2017 ISO 21528-1:2017 Escherichia coli 00012b / 00013g
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Buffered peptone water (BPW) (pre-enrichment)i
ISO 22964:2017 ISO 22964:2017 Cronobacter sakazakii 00214b
Cronobacter muytjensii 00213g
Buffered peptone water (single and double strength) (diluent)
ISO 6887-1:2017 ISO 6887-1:2017 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
CAMP mediumwith Staphylococcus aureus WDCM 00034
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11133:2014 / Amd 2:2020
Listeria monocytogenes 00020e / 00021e
Listeria ivanovii 00018e
Listeria innocua 00017f
CAMP mediumwith Rhodococcus equi WDCM 00028
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11133:2014 / Amd 2:2020
Listeria ivanovii 00018e
Listeria monocytogenes 00020f / 00021f
Listeria innocua 00017f
Carbohydrate-free agar medium for enumeration
ISO 13559:2002 - - -
Carbohydrate utilization broth (peptone water with bromocresol purple) with rhamnose
ISO 11290-1:2017 ISO 11133:2014 / Amd 2:2020
Listeria monocytogenes 00021e / 00109e
Listeria ivanovii 00018f
Carbohydrate utilization broth (peptone water with bromocresol purple) with xylose
ISO 11290-1:2017 ISO 11133:2014 / Amd 2:2020
Listeria ivanovii 00018e
Listeria monocytogenes 00021f / 00109f
Carbohydrate utilization broth (peptone water with bromocresol purple) with different carbohydrates
ISO 21567:2004 - - -
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 6
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Carbohydrate utilization broth (peptone water with phenol red) with salicin
ISO 10273:2017 ISO 10273:2017 Yersinia intermedia 00127e
Yersinia enterocolitica 00216f
Carbohydrate utilization broth (peptone water with phenol red) with trehalose
ISO 10273:2017 ISO 10273:2017
Yersinia enterocolitica 00216e
Yersinia enterocolitica 00160e
Yersinia intermedia 00217e
Carbohydrate utilization broth (peptone water with phenol red) with xylose
ISO 10273:2017 ISO 10273:2017
Yersinia enterocolitica 00160e
Yersinia intermedia 00127e
Yersinia enterocolitica 00216f
Carbohydrate utilization broth (peptone water with phenol red) with different carbohydrates
ISO 22964:2017 - - -
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 7
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Catalase reagent (3 % hydrogen peroxide solution)
ISO 9232:2003 ISO 10272-1:2017 ISO 10272-2:2017 ISO 11290-1:2017 ISO 11290-2:2017 ISO 13722:2017
ISO 10272-1:2017 ISO 10272-2:2017 ISO 11133:2014 / Amd 2:2020
Staphylococcus aureus 00032e / 00034e
Camplyobacter jejuni 00005e
Listeria monocytogenes 00020e / 00021e
Listeria innocua 00017e
Listeria ivanovii 00018e
Brochothrix thermosphacta 00071f
Enterococous faecalis 00009f / 00087f / 00176f
Enterococcus faecium 00177f / 00178f
Lactobacillus delbrueckii subsp. bulgaricus
00121f
Cefixime tellurite sorbitol MacConkey (CT-SMAC) agar
ISO 16654:2001 ISO 11133:2014
Escherichia coli O157:H7 00014b, h
Staphylococcus aureus 00032d / 00034d
Escherichia coli 00012d / 00013d
Cefsulodin, IrgasanTM (triclosan) and novobiocin (CIN) agar
ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00216b / 00160g
Escherichia coli 00012d / 00013d
Cephalothin fucidin cetrimide (CFC) agar
ISO 13720:2010 ISO 11133:2014
Pseudomonas fluorescens 00115b
Pseudomonas fragi 00116g
Escherichia coli 00012d / 00013d
Christensen’s citrate agar (citrate agar)
ISO 21567:2004 ISO 11133:2014 / Amd 2:2020
Enterobacter aerogenes 00175e
Enterobacter cloacae 00083e
Shigella sonnei 00127f
Shigella flexneri 00125f / 00126f
Chromogenic Cronobacter isolation (CCI) agar
ISO 22964:2017 ISO 22964:2017 Cronobacter sakazakii 00214b
Cronobacter muytjensii 00213g
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 8
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Staphylococcus aureus 00032d / 00034d
Enterobacter cloacae 00083b
Columbia blood agar ISO 10272-1:2017 ISO 10272-2:2017
ISO 10272-1:2017 ISO 10272-2:2017
Escherichia coli 00012d / 00013d
Camplyobacter jejuni / coli 00156d / 00005d/ 00004d
Cold enrichment broth (CEB) ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00160b
Pseudomonas aeruginosa 00025b
Congo red-magnesium oxalate (CR-MOX) agar
ISO 10273:2017 ISO 10273:2017
Campylobacter coli 00004b
Yersinia enterocolitica 00216e
Yersinia enterocolitica 00160f
Cronobacter selective broth (CSB)
ISO 22964:2017 ISO 22964:2017
Cronobacter sakazakii 00214b
Cronobacter muytjensii 00213g
Staphylococcus aureus 00032d / 00034d
Dichloran glycerol (DG 18) agar
ISO 21527-2:2008 ISO 11133:2014
Saccharomyces cerevisiae 00058b
Wallemia sebi 00182b
Aspergillus restrictus 00183g
Eurotium rubrum 00184g
Escherichia coli 00012f / 00013f, m
Bacillus subtilis subsp. spizizenii 00003f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 9
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Dichloran-rose bengal chloramphenicol (DRBC) agar
ISO 21527-1:2008 ISO 11133:2014
Saccharomyces cerevisiae 00058b
Aspergillus brasiliensis 00053b
Candida albicans 00054g
Mucor racemosus 00181g
Escherichia coli 00012f / 00013f, m
Bacillus subtilis subsp. spizizenii 00003f
Diluent for general use with α-amylase
ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Dipotassium hydrogen phosphate solution
ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Dipotassium hydrogen phosphate solution with antifoam agent
ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
EC broth (EC) ISO 7251:2005 ISO 11133:2014 Escherichia coli 00012b / 00013g
Pseudomonas aeruginosa 00025b
Fluid thioglycollate medium (Thioglycollate)
ISO 7937:2004 ISO 11133:2014 Clostridium perfringens 00007b
Fraser broth (Fraser) ISO 11290-1:2017 ISO 11290-1:2017
Listeria monocytogenes serovar 4b
00021b
Listeria monocytogenes serovar 1/2a
00109g
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 10
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Glucose agar ISO/TS 11059:2009 ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Pseudomonas aeruginosa 00024f / 00025f / 00026f
Pseudomonas fluorescens 00115f
Glucose OF medium (O-F medium with overlay)
ISO 21528-1:2017 ISO 21528-2:2017
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Pseudomonas aeruginosa 00024f / 00025f / 00026f
Pseudomonas fluorescens 00115f
Glucose MRS broth with overlay agar
ISO 9232:2003 ISO 11133:2014 / Amd 2:2020 Lactobacillus brevis 00099e
Half Fraser broth (Half Fraser)
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11290-1:2017
Listeria monocytogenes serovar 4b
00021b
Listeria monocytogenes serovar 1/2a
00109g
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Hektoen enteric (HE) agar ISO 21567:2004 - - -
Hippurate hydrolysis reagent
ISO 10272-1:2017 ISO 10272-2:2017
ISO 10272-1:2017 ISO 10272-2:2017
Camplyobacter jejuni 00005b
Camplyobacter coli 00004b
Hydrolysate of casein tryptone (HCT) agar medium
ISO 7932:2004 / Amd 1:2020
ISO 7932:2004 / Amd 1:2020 Bacillus thuringiensis 00221b
Bacillus cereus 00001b
Indoxyl acetate discs ISO 10272-1:2017 ISO 10272-2:2017
ISO 10272-1:2017 ISO 10272-2:2017
Camplyobacter jejuni 00005b
Camplyobacter lari 00204b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 11
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Indole reagent (Kovacs reagent)
ISO 11866-1:2005 ISO 11866-2:2005
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Enterobacter aerogenes 00175f
Citrobacter freundii 00006f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
Indole reagent (Vracko and Sheries reagent)
ISO 11866-1:2005 ISO 11866-2:2005
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Enterobacter aerogenes 00175f
Citrobacter freundii 00006f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
IrgasanTM, ticarcillin and potassium chlorate (ITC) broth
ISO 10273:2017 ISO 10273:2017 ISO 11133:2014 / Amd 1:2018
Yersinia enterocolitica 00216b / 00160g
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Proteus mirabilis 00023b
Iron sulfite (IS) agar (“Tryptose sulfite agar”, TS")
ISO 15213:2003 ISO 11133:2014 / Amd 1:2018 Clostridium perfringens 00007b / 00080g
Escherichia coli 00012d / 00013d
Lactosegelatin medium ISO 7937:2004 ISO 11133:2014 / Amd 2:2020 Clostridium perfringens 00007e
Hafnia alvei 00095f
Lactose sulfite (LS) medium ISO 7937:2004 ISO 11133:2014 / Amd 2:2020 Clostridium perfringens 00007e
Clostridium sporogenes 00008f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 12
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Lauryl sulfate tryptose (LST) broth
ISO 4831:2006 ISO 11133:2014
Escherichia coli 00012b / 00013g
Citrobacter freundii 00006g
Enterococcus faecalis 00009d / 00087d
Lauryl sulfate tryptose (LST) broth
ISO 7251:2005 ISO 11133:2014 Escherichia coli 00012b / 00013g
Enterococcus faecalis 00009d / 00087d
L-Lysine decarboxylation (LDC) medium
ISO 6579-1:2017 ISO 21567:2004 ISO 22964:2017
ISO 11133:2014 / Amd 2:2020
Salmonella Enteritidis 00030c, e
Salmonella Typhimurium 00031c, e
Enterobacter aerogenes 00175e
Proteus mirabilis 00023f
Citrobacter freundii 00006f
Cronobacter sakazakii 00214f
Cronobacter muytjensis 00213f
Escherichia coli 00012f / 00013f / 00090f / 00179f
L-lysine decarboxylase (LDC) saline medium
ISO 21872-1:2017 ISO 11133:2014 / Amd 2:2020 Vibrio parahaemolyticus 00185e
M17 medium ISO 7889:2003 - - -
MacConkey agar ISO 21567:2004 - - -
Malachite green oxalate solution
ISO 21871:2006 ISO 11133:2014 / Amd 2:2020 Bacillus cereus 00001e
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 13
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Mannitol egg yolk polymyxin (MYP) agar
ISO 7932:2004 ISO 21871:2006
ISO 11133:2014
Bacillus cereus 00001b
Escherichia coli 00012d / 00013d
Bacillus subtilis subsp. spizizenii 00003b
Methyl red (MR) / Voges-Proskauer (VP) medium with reagents
ISO 22964:2017 - - -
Microscopic aspect - Gram staining
ISO 7218:2007 ISO 11290-1:2017 ISO 11290-2:2017
- - -
Microscopic aspect - wet microscopy
ISO 7218:2007 ISO 11290-1:2017 ISO 11290-2:2017
- - -
Minerals-modified glutamate agar (MMGA)
ISO 16649-1:2018 ISO 16649-1:2018
Escherichia coli 00012d / 00013d, l/ 00202b, l
Enterococcus faecalis 00009d / 00087d
Citrobacter freundii 00006b
Pseudomonas aeruginosa 00025g
Minerals-modified glutamate agar (MMGA)
ISO 11866-2:2005 - - -
Minerals-modified glutamate medium (MMG)
ISO 16649-3:2015 ISO 16649-3:2015 Escherichia coli 00012b / 00013g
Enterococcus faecalis 00009d / 00087d
Modified charcoal cefoperazone deoxycholate agar (mCCDA)
ISO 10272-1:2017 ISO 10272-2:2017
ISO 10272-1:2017 ISO 10272-2:2017
Camplyobacter jejuni 00156d / 00005d
Campylobacter coli 00004g
Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034g
Modified Giolitti and Cantoni broth (Giolitti Cantoni)
ISO 6888-3:2003 ISO 11133:2014 Staphylococcus aureus 00034b / 00032g
Escherichia coli 00012d / 00013d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 14
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Modified lauryl sulfate tryptose broth (LST + MUG)
ISO 11866-2:2005 -
- -
- -
- -
Modified Rappaport broth (MRB)
ISO 10273:2017 ISO 10273:2017
Yersinia enterocolitica 00160b
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Proteus mirabilis 00023b
Modified semi-solid Rappaport-Vassiliadis (MSRV) agar
ISO 6579-1:2017 / Amd 1:2020 ISO/TS 6579-2:2012
ISO 6579-1:2017 ISO 11133:2014
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Modified tryptone soya broth (mTSB)
ISO 16654:2001 - - -
Motility medium - non-selective liquid medium including sterile water
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11133:2014 / Amd 2:2020
Listeria monocytogenes serovar 4b
00021e
Listeria monocytogenes serovar 1/2a
00109e
Listeria innocua 00017e
Staphylococcus aureus 00032f / 00034f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 15
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Motility medium - semisolid nutrient agar for motility test
ISO 11290-1:2017 ISO 11290-2:2017 ISO 21567:2004
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Listeria monocytogenes serovar 4b
00021e
Listeria monocytogenes serovar 1/2a
00109e
Listeria innocua 00017e
Shigella sonnei 00127f
MRS (de Man, Rogosa and Sharpe) agar medium
ISO 15214:1998 ISO 11133:2014
Lactobacillus sakei 00015b
Lactococcus lactis 00016b
Pediococcus pentosaceus 00158g
Escherichia coli 00012d / 00013d
Bacillus cereus 00001g
MRS agar medium, acidified ISO 7889:2003 - - -
MRS/clindamycin/ciprofloxacin agar (MRS/CL/CIP agar)
ISO 20128:2006 - - -
MRS broth - growth at 15 °C ISO 9232:2003 ISO 11133:2014 / Amd 2:2020
Lactobacillus casei 00100e
Lactobacillus plantarum 00104e
Lactobacillus delbrueckei subsp. bulgaricus
00102f
MRS broth - growth at 45 °C ISO 9232:2003 ISO 11133:2014 / Amd 2:2020
Lactobacillus delbrueckei subsp. bulgaricus
00102e
Lactobacillus plantarum 00104f
Mucate broth ISO 21567:2004 ISO 11133:2014 / Amd 2:2020 Escherichia coli
00012e / 00013e / 00090e / 00179e
Shigella flexneri 00125f / 00126f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 16
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Muller-Kauffmann tetrathionate-novobiocin (MKTTn) broth
ISO 6579-1:2017 ISO 6579-1:2017 / Amd 1:2020
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Enterococcus faecalis 00009d / 00087d
Nickels and Leesman agar medium with X-gal
ISO 17992:2006 - - -
Nickels and Leesman agar medium with vancomycin
ISO 17992:2006 - - -
Nitrate motility medium
ISO 7937:2004 ISO 11133:2014 / Amd 2:2020
Clostridium perfringens 00007e
Clostridium sporogenes 00008f
Clostridium bifermentans 00079f
Nutrient agari ISO 6579-1:2017 ISO 6579-1:2017 Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Nutrient agari ISO/TS 6579-2:2012 ISO 11133:2014 Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Nutrient agari ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00216b / 00160g
Nutrient agari
ISO 16654:2001 ISO 21528-1:2017 ISO 21528-2:2017 ISO 21567:2004
ISO 11133:2014 ISO 21528-1:2017 ISO 21528-2:2017
Escherichia coli 00012b / 00013g
Nutrient agari ISO/TS 11059:2009 ISO/TS 11059:2009
Pseudomonas fluorescens 00115e
Pseudomonas aeruginosa 00025e
Escherichia coli 00012e
Staphylococcus aureus 00034e
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 17
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Ornithine decarboxylase medium
ISO 21567:2004 ISO 22964:2017
ISO 11133:2014 / Amd 2:2020
Proteus mirabilis 00023e
Enterobacter aerogenes 00175e
Cronobacter sakazakii 00214e
Cronobacter muytjensis 00213e
Shigella sonnei 00127e
Citrobacter freundii 00006f
Pseudomonas aeruginosa 00024f / 00025f / 00026f
Pseudomonas fluorescens 00115f
Oxidase reagent
ISO 10272-1:2017 ISO 10272-2:2017 ISO/TS 11059:2009 ISO 13720:2010 ISO 13722:2017 ISO 21528-1:2017 ISO 21528-2:2017 ISO 21872-1:2017 ISO 22964:2017
ISO 10272-1:2017 ISO 10272-2:2017 ISO 11133:2014 / Amd 2:2020
Camplyobacter jejuni 00005e
Pseudomonas aeruginosa 00024e / 00025e / 00026e
Pseudomonas fluorescens 00115e
Vibrio parahaemolyticus 00185e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Cronobacter sakazakii 00214f
Cronobacter muytjensis 00213f
Brochothrix thermosphacta 00071f
Penicillin and pimaricin agar (PPA)
ISO/TS 11059 ISO 11133:2014
Pseudomonas fluorescens 00115b
Pseudomonas aeruginosa 00025g
Escherichia coli 00012d / 00013d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 18
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Peptone water, indole free with indole reagent (Kovacs reagent)
ISO 7251:2005 ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Enterobacter aerogenes 00175f
Citrobacter freundii 00006f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
Peptone solution ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Peptone salt solution ISO 6887-1:2017 ISO 6887-1:2017 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Peptone solution ISO 6887-1:2017 ISO 6887-1:2017 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Peptone, sorbitol and bile salts broth (PSB)
ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00216b / 00160g
Pseudomonas aeruginosa 00025b
Peptone waters with different NaCl concentration: 0 %
ISO 21871-2017 ISO 11133:2014 / Amd 2:2020
Vibrio cholerae non O1/non O139
00203e
Vibrio parahaemolyticus 00185f
Peptone waters with different NaCl concentration: 6 %
ISO 21871-2017 ISO 11133:2014 / Amd 2:2020
Vibrio parahaemolyticus 00037e / 00138e / 00185e
Vibrio vulnificus 00139e
Vibrio cholerae non O1/non O139
00203f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 19
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Peptone waters with different NaCl concentration: 10 %
ISO 21871-2017 ISO 11133:2014 / Amd 2:2020
Staphylococcus aureus 0032e / 0034e
Vibrio cholerae non O1/non O139
00203f
Vibrio parahaemolyticus 00037f / 00138f / 00185f
Vibrio vulnificus 00139f
Phosphate buffered diluent ISO 6887-1:2017 ISO 6887-1:2017 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Phosphate buffer solution ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Plate count agar (PCA) ISO 4833-1:2013 ISO 11133:2014
Bacillus subtilis subsp. spizizenii 00003b
Escherichia coli 00012b / 00013g
Staphylococcus aureus 00034g
Plate count skim milk agar (MPCA)
ISO 4833-1:2013 ISO 11133:2014
Bacillus subtilis subsp. spizizenii 00003b
Escherichia coli 00012b / 00013g
Staphylococcus aureus 00034g
Plate count agar (PCA) ISO 17410:2019 ISO 17410:2019 Pseudomonas fluorescens 00115b
Yersinia enterocolitica subsp. palaearctica
00216g
Plate count milk agar ISO 8553:2004 - - -
Plate count skim milk agar (MPCA)
ISO 17410:2019 ISO 17410:2019 Pseudomonas fluorescens 00115b
Yersinia enterocolitica subsp. palaearctica
00216g
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 20
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) agar
ISO 21871:2006 ISO 11133:2014
Bacillus cereus 00001b
Escherichia coli 00012d / 00013d
Bacillus subtilis subsp. spizizenii 00003b
Preston broth ISO 10272-1:2017 ISO 10272-1:2017
Camplyobacter jejuni 00156d / 00005d
Campylobacter coli 00004g
Escherichia coli 00012d / 00013d
Proteus mirabilis 00023b
Pyrazinamidase reaction (Casein-soya agar with pyrazin and ammonium iron (II) sulfate solution)
ISO 10273:2017 ISO 10273:2017
Yersinia enterocolitica 00160e
Yersinia intermedia 00217e
Yersinia enterocolitica 00216f
Quarter-strength Ringer's solution
ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Rabbit plasma ISO 6888-1:1999 ISO 6888-3:2003
ISO 11133:2014 / Amd 2:2020
Staphylococcus aureus 00032e / 00034e / 00035e, k
Staphylococcus epidermidis 00036f
Staphylococcus saprophyticus 00159f
Rabbit plasma fibrinogen agar (RPFA)
ISO 6888-1:1999 / Amd 1:2018 ISO 6888-2:1999 ISO 6888-3:2003
ISO 6888-1:1999 / Amd 1:2018 ISO 11133:2014
Staphylococcus aureus 00032g / 00034b
Escherichia coli 00012d / 00013d
Staphylococcus saprophyticus 00159b
Staphylococcus epidermidis 00036g
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 21
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Rappaport-Vassiliadis medium with soya (RVS broth)
ISO 6579-1:2017 ISO 6579-1:2017
Salmonella Enteritidis / Typhimurium
00030c,d / 00031c,d
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Enterococcus faecalis 00009d / 00087d
Sabouraud dextrose agar (SDA) (reference medium)
ISO 11133:2014 ISO 1133:2014 Saccharomyces cerevisiae 00058b
Aspergillus brasiliensis 00053b
Safranin solution ISO 21871:2006 ISO 11133:2014 / Amd 2:2020 Bacillus cereus 00001e
Saline nutrient agar (SNA) ISO 21872-1:2017 - - -
Saline solution with toluene and β-Galactosidase reagent (ONPG)
ISO 6579-1:2017 ISO 21567:2004 ISO 21872-1:2017
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Proteus mirabilis 00023f
Vibrio parahaemolyticus 00185f
Selenite cystine (SC) medium ISO 6579-1:2017 / Amd 1:2020
ISO 6579-1:2017 / Amd 1:2020
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Pseudomonas aeruginosa 00025b
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Sheep blood agar for haemolysis test
ISO 7932:2004 ISO 11290-1:2017 ISO 11290-2:2017 ISO 21871:2006
ISO 11133:2014 / Amd 2:2020
Bacillus cereus 00001e
Listeria monocytogenes 00021e / 00109e
Bacillus subtilis subsp. spizizenii 00003f
Bacillus subtilis 00070f
Listeria innocua 00017f
Shigella broth ISO 21567:2004 - - -
Simmon's citrate medium ISO 10273:2017 ISO 10273:2017 Yersinia intermedia 00217e
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 22
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Yersinia enterocolitica 00216f
Yersinia enterocolitica 00160f
Sodium acetate agar (acetate agar)
ISO 21567:2004 ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Shigella sonnei 00127f
Shigella flexneri 00125f
Sodium citrate solution ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Streptomycin/thallium acetatet agar (STAA)
ISO 13722:2017 ISO 13722:2017
Brochothrix thermosphacta 00071b
Enterococcus faecalis 00009b
Lactobacillus sakei 00015b
Sudan black B solution ISO 21871:2006 ISO 11133:2014 / Amd 2:2020 Bacillus cereus 00001e
Bacillus subtilis 00070f
Sulfite cycloserine agar/ tryptose sulphite cycloserine (TSC/SC) agar without egg yolk
ISO 7937:2004 ISO 11133:2014 / Amd 1:2018 Clostridium perfringens 00007b / 00080g
Escherichia coli 00012d / 00013d
Thiosulfate citrate bile salts sucrose (TCBS) agar
ISO 21872-1:2017 ISO 21872-1:2017
Vibrio parahaemolyticus 00185b
Vibrio furnissii 00186b
Escherichia coli 00012e, n / 00013e, n / 00090e, n
Toluidine blue O-DNA agar ISO 8870:2006 - - -
TOS-MUP agar medium ISO 29981:2010 - - -
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 23
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Triple sugar/iron (TSI) agar ISO 6579-1:2017 ISO 21567:2004
ISO 11133:2014 / Amd 2:2020
Salmonella Enteritidis 00030c, e
Salmonella Typhimurium 00031c, e
Shigella sonnei 00127e
Shigella flexneri 00125e
Pseudomonas aeruginosa 00024f / 00025f / 00026f
Tripolyphosphate solution ISO 6887-5:2020 ISO 6887-5:2020 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Tryptic soy broth (TSB) (tryptone soya broth, casein soya bean digest medium)
ISO 10273:2017 - - -
Tryptone soya agar (TSA) (reference medium)
ISO 1133:2014 ISO 11133:2014
Bacillus cereus 00001o
Bacillus subtilis subsp. spizizenii 00003o
Escherichia coli 00012o
Listeria monocytogenes serovar 4b
00021o
Staphylococcus aureus 00034o
Tryptone bile X-glucuronide (TBX) agar
ISO 16649-1:2018 ISO 16649-2:2001 ISO 16649-3:2015
ISO 16649-1:2018 ISO 11133:2014 / Amd 1:2018
Escherichia coli 00012d / 00013d, l/ 00202b, l
Enterococcus faecalis 00009d / 00087d
Citrobacter freundii 00006b
Pseudomonas aeruginosa 00025g
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 24
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Tryptone soya agar (TSA) (example of non-selective medium)
ISO 22964:2017 - - -
Tryptone soya agar (TSA) (reference medium)
ISO 11133:2014 ISO 11133:2014
Bacillus cereus 00001o
Bacillus subtilis subsp. spizizenii 00003o
Escherichia coli 00012o
Listeria monocytogenes serovar 4b
00021o
Staphylococcus aureus 00034o
Tryptone soya yeast extract agar (TSYEA)
ISO 11290-1:2017 ISO 11290-2:2017
ISO 11133:2014
Listeria monocytogenes serovar 4b
00021b
Listeria monocytogenes serovar 1/2a
00109g
Tryptone soya polymyxin broth (TSPB)
ISO 21871:2006 ISO 11133:2014 Bacillus cereus 00001b
Escherichia coli 00012d / 00013d
Tryptone /tryptophan medium with indole reagent (Kovacs reagent)
ISO 6579-1:2017 ISO 16654:2001 ISO 21567:2004
ISO 11133:2014 / Amd 2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Enterobacter aerogenes 00175f
Citrobacter freundii 00006f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
Tryptone / tryptophan medium with indole reagent (Kovacs reagent)
ISO 10273:2017 ISO 10273:2017
Yersinia enterocolitica 00160e
Yersinia intermedia 00217e
Yersinia enterocolitica 00216f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 25
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Tryptone / tryptophan saline medium with indole reagent (Kovacs reagent)
ISO 21872-1:2017 ISO 11133:2014 / Amd 2:2020
Vibrio parahaemolyticus 00037e / 00138e / 00185e
Vibrio cholerae 00203e
Vibrio vulnificus 00139e
Tryptone-bile agar ISO 11866-2:2005 - - -
Tween-esterase test medium ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00160e
Yersinia enterocolitica 00216f
Urea agar (Christensen) ISO 6579-1:2017 ISO 21567:2004
ISO 11133:2014 / Amd 2:2020
Proteus mirabilis 00023e
Klebsiella pneumoniae 00097e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
Shigella sonnei 00127f
Shigella flexneri 00125f
Urea agar (Christensen) ISO 10273:2017 ISO 10273:2017 Yersinia enterocolitica 00216e / 00160e
Violet red bile glucose (VRBG) agar
ISO 21528-1:2017 ISO 21528-2:2017
ISO 21528-1:2017 ISO 21528-2:2017
Escherichia coli 00012b / 00013g
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Enterococcus faecalis 00009d / 00087d
Violet red bile lactose (VRBL) agar
ISO 4832:2006 ISO 11133:2014
Escherichia coli 00012b / 00013g
Enterococcus faecalis 00009d / 00087d
Pseudomonas aeruginosa 00025b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 26
Table 3 — Control strains for culture media and reagents from ISO/TC 34/SC 9 & SC 5 standards (food microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Voges-Proskauer medium with reagents
ISO 11290-1:2017 ISO 11290-2:2017
ISO11133:2014 / Amd 2:2020
Listeria monocytogenes serovar 4b
00021e
Listeria monocytogenes serovar 1/2a
00109e
Listeria innocua 00017e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Xylose Lysine deoxycholate (XLD) agar
ISO 6579-1:2017 / Amd1:2020 ISO/TS 6579-2:2012
ISO 6579-1:2017 / Amd 1:2020 ISO 11133:2014
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Xylose lysine deoxycholate (XLD) agar
ISO 21567:2004 - - -
Yeast extract dextrose oxytetracycline agar medium
ISO 6611:2004 - - -
Yeast extract dextrose chloramphenicol agar medium
ISO 6611:2004 - - -
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 27
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Acetamide broth with Nessler’s reagent
ISO 16266:2006 ISO 11133:2014 / Amd2:2020 Pseudomonas aeruginosa
00024e / 00025e / 00026e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Acid phosphatase reagent ISO 14189:2013 ISO 11133:2014 / Amd2:2020 Clostridium perfringens
00007e / 00080e / 00174e
Clostridium bifermentans 00079f
Bile aesculin azide agar ISO 7899-1:2000 ISO 11133:2014 / Amd 2:2020
Enterococcus faecalis 00009e / 00176e
Enterococcus faecium 00177e / 00178e
Aerococcus viridans 00061f
Escherichia coli 00012f / 00013f / 00090f / 00179f
Blood agar ISO 11731:2017 ISO 11731:2017 Legionella pneumophila 00107b
Legionella pneumophila 00180g
Blood agar ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
Blood agar (or other suitable base with 5% blood)
ISO 14189:2013 - - -
Bolton broth ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
Escherichia coli 00012d / 00013d
Staphylococcus aureus 00032d / 00034d
Buffered charcoal yeast extract (BCYE) agar
ISO 11731:2017 ISO 11731:2017 Legionella pneumophila 00107b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 28
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Buffered charcoal yeast extract agar without L-cysteine (BCYE–cys)
ISO 11731:2017 ISO 11731:2017 Legionella pneumophila 00107b
Legionella pneumophila 00180g
Buffered charcoal yeast extract agar with selective supplements (BCYE+AB)
ISO 11731:2017 ISO 11731:2017
Legionella pneumophila 00107b
Legionella pneumophila 00180g
Legionella anisa 00106g
Enterococcus faecalis 00009d / 00087d
Escherichia coli 00012d / 00013d
Buffered peptone water (BPW) (pre-enrichment)
ISO 19250:2010 ISO 11133:2014 Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Catalase ISO 17995:2019 ISO 17995:2019 Campylobacter jejuni 00005e
Enterococcus faecalis 00085f / 00087f
Chromogenic Coliform agar (CCA)
ISO 9308-1:2014 ISO 9308-1:2014 / Amd1:2016
Escherichia coli 00012d / 00013d
Enterobacter aerogenes 00175e
Citrobacter feundii 00006e
Enterococcus faecalis 00009d / 00087d
Pseudomonas aeruginosa 000024b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 29
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Colilert-18 medium ISO 9308-2:2012 ISO 11133:2014 / Amd 1:2018
Escherichia coli 00013b/ 00090g
Klebsiella variicola 00206b
Pseudomonas aeruginosa 00024d / 00025d
Columbia agar (with or without blood)
ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
Differential reinforced clostridial medium (DRCM)
ISO 6461-1:1986 ISO 11133:2014
Clostridium perfringens 00007b
Clostridium perfringens 00080g
Escherichia coli 00012d / 00013d
Glycine vancomycin polymyxin B cycloheximide (GVPC) agar
ISO 11731:2017 ISO 11731:2017 ISO 11133:2014 / Amd1:2018
Legionella pneumophila 00107b
Legionella pneumophila 00180g
Legionella anisa 00106g
Enterococcus faecalis 00009d / 00087d
Escherichia coli 00012d / 00013d
Hippurate hydrolysis ISO 17995:2019 ISO 17995:2019 Campylobacter jejuni 00005e
Campylobacter jejuni 00004f
Indoxyl acetate hydrolysis ISO 17995:2019 ISO 17995:2019 Campylobacter jejuni 00005e
Campylobacter lari 00204f
King’s B medium ISO 16266:2006 ISO 11133:2014 / Amd2:2020 Pseudomonas aeruginosa
00024e / 00025e / 00026e
Escherichia coli 00012f / 00013f / 00090f / 00179f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 30
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
KOH (3 % potassium hydroxide solution)
ISO 17995:2019 ISO 11133:2014 / Amd2:2020
Escherichia coli 00012e / 00013e / 00090e / 00179e
Campylobacter jejuni 00156e / 00005e
Campylobacter coli 00004e
Staphylococcus aureus 00032f / 00034f
L-Lysine decarboxylation (LDC) medium
ISO 19250:2010 ISO 11133:2014 / Amd 2:2020
Salmonella Enteritidis 00030c, e
Salmonella Typhimurium 00031c, e
Enterobacter aerogenes 00175e
Proteus mirabilis 00023f
Citrobacter freundii 00006f
Cronobacter sakazakii 00214f
Cronobacter muytjensis 00213f
Escherichia coli 00012f / 00013f / 00090f / 00179f
Modified-charcoal cefoperazone desoxycholate agar (mCCDA)
ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
Escherichia coli 00012d / 00013d
Staphylococcus aureus 00032d / 00034d
mCCDA basal medium without supplements
ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 31
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Microscopic aspect - motility and cell morphology
ISO 17995:2019 ISO 17995:2019 Campylobacter jejuni 00005e
Escherichia coli 00012f
Modified Wadowsky Yee (MWY) agar
ISO 11731:2017 ISO 11731:2017
Legionella pneumophila 00107b
Legionella pneumophila 00180g
Legionella anisa 00106g
Enterococcus faecalis 00009d / 00087d
Escherichia coli 00012d / 00013d
MUD/SF medium (4-methylumbelliferyl-α-D glucoside /SF medium)
ISO 7899-1:1998 ISO 7899-1:1998
Enterococcus faecalis 00176g
Enterococcus hirae 00089g
Enterococcus faecium 00178g
Aerococcus viridans 00061g
Lactococcus lactis 00016g
Staphylococcus epidermidis 00132g
MUG/EC medium (4-methylumbelliferyl-β-D glucuronide /EC medium)
ISO 9308-3:1998 ISO 9308-3:1998 Escherichia coli 00179b
Muller-Kauffmann tetrathionate-novobiocin (MKTTn) broth
ISO 19250:2010 ISO 11133:2014 / Amd1:2018
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Enterococcus faecalis 00009d / 00087d
Nutrient agar ISO 11731:2017 ISO 11731:2017 Legionella pneumophila 00107b
Legionella pneumophila 00180g
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 32
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Oxidase reagent ISO 9308-1:2014 ISO 16266:2006 ISO 17995:2019
ISO 11133:2014 / Amd2:2020 ISO 17995:2019
Camplyobacter jejuni 00005e
Pseudomonas aeruginosa 00024e / 00025e / 00026e
Pseudomonas fluorescens 00115e
Vibrio parahaemolyticus 00185e
Campylobacter jejuni 00005e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Cronobacter sakazakii 00214f
Cronobacter muytjensis 00213f
Brochothrix thermosphacta 00071f
Page’s saline ISO 11731:2017 - - -
Peptone diluent ISO 8199:2018 ISO 8199:2018 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Peptone saline solution ISO 8199:2018 ISO 8199:2018 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Phosphate buffer solution ISO 8199:2018 ISO 8199:2018 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Phosphate-buffered saline (PBS) [pH 7,5]
ISO 11731:2017 - - -
Preston broth ISO 17995:2019 ISO 17995:2019
Campylobacter jejuni 00005d / 00156d
Campylobacter coli 00072d / 00004d
Campylobacter lari 00204b
Escherichia coli 00012d / 00013d
Staphylococcus aureus 00032d / 00034d
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 33
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Pseudalert medium ISO 16266-2:2018 ISO 16266-2:2018 Pseudomonas aeruginosa 00024d / 00025d
Pseudomonas fluorescens 00115b
Pseudomonas cetrimide nalidixic acid (CN) agar
ISO 16266:2006 ISO 11133:2014
Pseudomonas aeruginosa 00024b / 00025g / 0026g
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Quarter-strength Ringer’s solution
ISO 8199:2018 ISO 11731:2017
ISO 8199:2018 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Rappaport-Vassiliadis medium with soya (RVS broth)
ISO 19250:2010 ISO 11133:2014
Salmonella Enteritidis / Typhimurium
00030c,d / 00031c,d
Escherichia coli 00012d / 00013d
Pseudomonas aeruginosa 00025b
Enterococcus faecalis 00009d / 00087d
Saline solution ISO 8199:2018 ISO 8199:2018 Escherichia coli 00012d / 00013d
Staphylococcus aureus 00034b
Slanetz and Bartley medium ISO 7899-2:2000 ISO 11133:2014
Enterococcus faecalis 00009b
Enterococcus faecalis 00087g
Enterococcus faecalis 00176g
Enterococcus faecium 00177d / 00178d
Escherichia coli 00012d / 00013d
Staphylococcus aureus 00032d / 00034d
Special diluent (SD) ISO 7899-1:1998 ISO 9308-3:1998
- - -
Sterile tap water ISO 11731:2017 - - -
ISO 6461-2:1986 ISO 11133:2014 / Amd1:2018 Clostridium perfringens 00007b
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 34
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Sulfite Iron [Tryptose Sulfite (TS)] agar
Clostridium perfringens 00080g
Escherichia coli 00012d / 00013d
Triple sugar/iron (TSI) agar ISO 19250:2010 ISO 11133:2014 / Amd 2:2020
Salmonella Enteritidis 00030c, e
Salmonella Typhimurium 00031c, e
Shigella sonnei 00127e
Shigella flexneri 00125e
Pseudomonas aeruginosa 00024f / 00025f / 00026f
Tryptone soy agar (TSA) ISO 11731:2017 ISO 11731:2017 Legionella pneumophila 00107b
Legionella pneumophila 00180g
Tryptose sulphite cycloserine (TSC) agar
ISO 14189:2013 ISO 11133:2014 / Amd1:2018
Clostridium perfringens 00007b
Clostridium perfringens 00080g / 00174g
Bacillus subtilis subsp. spizizenii 00003b
Urea agar (Christensen) ISO 19250:2010 ISO 11133:2014 / Amd 2:2020
Proteus mirabilis 00023e
Klebsiella pneumoniae 00097e
Escherichia coli 00012f / 00013f / 00090f / 00179f
Salmonella Enteritidis 00030c, f
Salmonella Typhimurium 00031c, f
Shigella sonnei 00127f
Shigella flexneri 00125f
List of control strains for performance testing of culture media and reagents
First edition published by 2020-10-12 35
Table 4 — Test strains for culture media and reagents from ISO/TC147/SC4 standards (water microbiology)
Medium/reagent Standard specifying
the medium/reagent Standard specifying
the performance testing Control strains
specified by the standard WDCM numbersa
Xylose Lysine deoxycholate (XLD) agar
ISO 19250:2010 ISO 11133:2014
Salmonella Enteritidis / Typhimurium
00030c, d / 00031c, d
Escherichia coli 00012d / 00013d
Enterococcus faecalis 00009d / 00087d
Yeast extract agar (YEA) ISO 6222:1999 ISO 11133:2014 Escherichia coli 00012d / 00013d
Bacillus subtilis subsp. spizizenii 00003g
